Prupe.3G300400_v2.0.a1

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. Functions as Fe-S scaffold, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Essential for embryo development

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
prunus_persica
Pp03
OG0012080
Physical Location & Seq
Forward (+)
26543448 .. 26546554
3107 bp
Loading structure...
UTR
Exon/CDS
Intron
Prupe.3G300400.2

Sequence Viewer

Length: 1071 bp
ATGGAGAACGGTGAAATTCCCGAAAACGCCAATGAACATTGCCCGGGTCCTCAATCCGATTCTGCTGGAAAATCTGATGCGTGCGAAGGATGCCCGAATCAACAAATTTGTGCTACTGCTCCAAAAGGCCCTGACCCAGACGTGGTTGCAATTGCAGAAAGGATGGCCACTGTAAAGCATAAGATACTGGTTTTATCAGGAAAAGGTGGAGTTGGCAAGAGCACATTCTCCGCCCAACTTGCATTTGCACTAGCAGCTATGGACTTCCAGGTGGGTCTACTTGACATTGACATTTGTGGCCCGAGCATCCCAAAGATGCTTGGCCTAGAAGGCCAAGAAATCCACCAGAGCAACCTTGGCTGGTCTCCTGTATATGTTGAGTCCAACCTTGGGGTCATGTCAATTGGATTCATGCTTCCAGAACCTGATGACGCTGTTATATGGAGGGGGCCGCGCAAAAATGCGCTCATTAAGCAATTCTTGAGGGATGTTTATTGGGGTGAGCTTGATTTTCTAGTAGTTGATGCTCCTCCTGGGACCTCAGATGAGCACATTTCAATTGTTCAATGCCTTGATGCTGGTTCAATAGACGGTGCAATCATAGTCACCACTCCACAACAGGTCTCCCTGATTGATGTTCGGAAAGAAGTAAGTTTCTGCAAGAAAGTTGGAGTTCAGGTCCTTGGGATTGTTGAGAATATGAGTGGCTTGTGCCAGCCAGTGATGGATTTCAAATTTTTGAGGATGACAGAGGCCGGTGAGCAAATAGACGTCACAGAATGGGCAAGGGAGCATCTGAAAGAGAGAGCGCCGGAATTTCTAAGCATAATAGCTTGCACTGAAGTGTTTGATAGTAGCCGTGGTGGAGCTGAAAAAATGTGCAGGGAAATGGGGGTACCTTTTCTGGGGAAGGTACCATTGGACCCCCAGCTTTGCAAGGCAGCTGAAGAAGGTAGATCTTGTTTTGTTGATCAGAAGTGCGGGGTGAGTGCACCTGCACTGAAGAAAGTCATAGATAAAATGATGGAAAATCAGGGATTATCTAGAATGTTGGTAGACAACAATGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0042802 GO:0042803 GO:0044424 GO:0044444 GO:0044464 GO:0046983 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ParA

Protein Analysis

357

Amino Acids

38.46

Weight (kDa)

4.81

Isoelectric Point (pI)

37.4

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50960
fragaria_vesca FvH4_6g52730
malus_domestica MD09G1017000.v1.1 MD17G1017500.v1.1
prunus_persica Prupe.3G300400_v2.0.a1 Prupe.3G300400_v2.0.a1
pyrus_communis pycom111g01380 pycom17g01410
rosa_chinensis RchiOBHm_Chr2g0174341
rosa_laevigata RLG00000022259
rosa_multiflora Rmu_sc0003649.1_g000032 Rmu_sc0007150.1_g000002
rosa_roxburghii Rroxscaffold_2G00078280
rosa_rugosa Rorug02G0576400
rosa_samantha Rh2AG657300 Rh2BG668300 Rh2CG631400 Rh2DG681900
rosa_wichuraiana Rw2G053830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 1003
AatII GACGTC 1 cut(s) 774
Acc36I ACCTGC 1 cut(s) 1003
Acc65I GGTACC 2 cut(s) 895, 913
AccB1I GGYRCC 2 cut(s) 895, 913
AccI GTMKAC 2 cut(s) 277, 1056
AccII CGCG 1 cut(s) 454
AciI CCGC 3 cut(s) 231, 452, 981
AcoI YGGCCR 1 cut(s) 165
AcsI RAATTY 4 cut(s) 15, 105, 734, 815
AcuI CTGAAG 3 cut(s) 861, 966, 1022
AcyI GRCGYC 1 cut(s) 771
AfaI GTAC 2 cut(s) 897, 915
AfiI CCNNNNNNNGG 3 cut(s) 142, 390, 905
AgsI TTSAA 4 cut(s) 558, 566, 585, 733
AjiI CACGTC 1 cut(s) 142
AjnI CCWGG 2 cut(s) 267, 532
AjuI GAANNNNNNNTTGG 2 cut(s) 902, 934
AleI CACNNNNGTG 1 cut(s) 842
AluBI AGCT 6 cut(s) 257, 505, 833, 869, 931, 944
AluI AGCT 6 cut(s) 257, 505, 833, 869, 931, 944
Alw21I GWGCWC 3 cut(s) 224, 552, 994
Alw26I GTCTC 2 cut(s) 369, 628
Alw44I GTGCAC 1 cut(s) 990
AlwNI CAGNNNCTG 1 cut(s) 425
Ama87I CYCGRG 2 cut(s) 43, 301
AoxI GGCC 7 cut(s) 127, 165, 298, 322, 331, 449, 753
ApaLI GTGCAC 1 cut(s) 990
ApeKI GCWGC 2 cut(s) 254, 941
ApoI RAATTY 4 cut(s) 15, 105, 734, 815
Asp718I GGTACC 2 cut(s) 895, 913
AspLEI GCGC 3 cut(s) 456, 466, 811
AspS9I GGNCC 7 cut(s) 47, 128, 299, 449, 537, 679, 922
AsuC2I CCSGG 2 cut(s) 44, 45
AsuHPI GGTGA 5 cut(s) 23, 512, 598, 770, 997
AvaI CYCGRG 2 cut(s) 43, 301
AvaII GGWCC 4 cut(s) 47, 537, 679, 922
BaeGI GKGCMC 1 cut(s) 994
BalI TGGCCA 1 cut(s) 167
BanI GGYRCC 2 cut(s) 895, 913
Bbv12I GWGCWC 3 cut(s) 224, 552, 994
BbvI GCAGC 2 cut(s) 266, 953
BccI CCATC 3 cut(s) 157, 718, 1018
BceAI ACGGC 1 cut(s) 843
BciT130I CCWGG 2 cut(s) 269, 534
BclI TGATCA 1 cut(s) 970
BcnI CCSGG 2 cut(s) 44, 45
BcoDI GTCTC 2 cut(s) 369, 628
BfaI CTAG 4 cut(s) 251, 326, 515, 1044
BfoI RGCGCY 1 cut(s) 812
BfuAI ACCTGC 1 cut(s) 1003
BglI GCCNNNNNGGC 1 cut(s) 330
BglII AGATCT 1 cut(s) 956
BisI GCNGC 3 cut(s) 255, 452, 942
BlsI GCNGC 3 cut(s) 256, 453, 943
Bme1390I CCNGG 4 cut(s) 44, 45, 269, 534
Bme18I GGWCC 4 cut(s) 47, 537, 679, 922
BmeT110I CYCGRG 2 cut(s) 43, 301
BmgBI CACGTC 1 cut(s) 142
BmgT120I GGNCC 7 cut(s) 47, 128, 299, 449, 537, 679, 922
BmiI GGNNCC 6 cut(s) 48, 450, 538, 897, 915, 924
BmrFI CCNGG 4 cut(s) 44, 45, 269, 534
BmsI GCATC 7 cut(s) 67, 80, 306, 315, 514, 565, 802
BpuEI CTTGAG 1 cut(s) 502
BpuMI CCSGG 2 cut(s) 44, 45
BsaHI GRCGYC 1 cut(s) 771
BsaI GGTCTC 2 cut(s) 369, 628
BsaJI CCNNGG 6 cut(s) 43, 355, 388, 533, 682, 859
BsaXI ACNNNNNCTCC 2 cut(s) 663, 693
Bsc4I CCNNNNNNNGG 3 cut(s) 142, 390, 905
Bse118I RCCGGY 1 cut(s) 755
Bse1I ACTGG 2 cut(s) 192, 719
Bse3DI GCAATG 1 cut(s) 37
BseBI CCWGG 2 cut(s) 269, 534
BseDI CCNNGG 6 cut(s) 43, 355, 388, 533, 682, 859
BseGI GGATG 5 cut(s) 95, 168, 306, 493, 750
BseLI CCNNNNNNNGG 3 cut(s) 142, 390, 905
BseMI GCAATG 1 cut(s) 37
BseMII CTCAG 1 cut(s) 555
BseNI ACTGG 2 cut(s) 192, 719
BseRI GAGGAG 1 cut(s) 519
BseSI GKGCMC 1 cut(s) 994
BseXI GCAGC 2 cut(s) 266, 953
BseYI CCCAGC 1 cut(s) 927
BsgI GTGCAG 2 cut(s) 901, 981
Bsh1236I CGCG 1 cut(s) 454
BshFI GGCC 7 cut(s) 129, 167, 300, 324, 333, 451, 755
BshNI GGYRCC 2 cut(s) 895, 913
BsiHKAI GWGCWC 3 cut(s) 224, 552, 994
BsiHKCI CYCGRG 2 cut(s) 43, 301
BsiSI CCGG 3 cut(s) 44, 756, 812
BslFI GGGAC 1 cut(s) 550
BslI CCNNNNNNNGG 3 cut(s) 142, 390, 905
BsmAI GTCTC 2 cut(s) 369, 628
BsmFI GGGAC 1 cut(s) 550
BsnI GGCC 7 cut(s) 129, 167, 300, 324, 333, 451, 755
Bso31I GGTCTC 2 cut(s) 369, 628
BsoBI CYCGRG 2 cut(s) 43, 301
Bsp1286I GDGCHC 3 cut(s) 224, 552, 994
Bsp143I GATC 2 cut(s) 956, 970
BspACI CCGC 3 cut(s) 231, 452, 981
BspANI GGCC 7 cut(s) 129, 167, 300, 324, 333, 451, 755
BspCNI CTCAG 1 cut(s) 554
BspFNI CGCG 1 cut(s) 454
BspLI GGNNCC 6 cut(s) 48, 450, 538, 897, 915, 924
BspMI ACCTGC 1 cut(s) 1003
BspT107I GGYRCC 2 cut(s) 895, 913
BspTNI GGTCTC 2 cut(s) 369, 628
BsrDI GCAATG 1 cut(s) 37
BsrFI RCCGGY 1 cut(s) 755
BsrI ACTGG 2 cut(s) 192, 719
BssAI RCCGGY 1 cut(s) 755
BssECI CCNNGG 6 cut(s) 43, 355, 388, 533, 682, 859
BssMI GATC 2 cut(s) 956, 970
BssNI GRCGYC 1 cut(s) 771
BssT1I CCWWGG 3 cut(s) 355, 388, 682
Bst2UI CCWGG 2 cut(s) 269, 534
Bst4CI ACNGT 3 cut(s) 11, 172, 593
BstACI GRCGYC 1 cut(s) 771
BstC8I GCNNGC 3 cut(s) 82, 716, 835
BstDEI CTNAG 2 cut(s) 541, 821
BstDSI CCRYGG 1 cut(s) 859
BstF5I GGATG 5 cut(s) 95, 168, 306, 493, 750
BstFNI CGCG 1 cut(s) 454
BstH2I RGCGCY 1 cut(s) 812
BstHHI GCGC 3 cut(s) 456, 466, 811
BstKTI GATC 2 cut(s) 959, 973
BstMAI GTCTC 2 cut(s) 369, 628
BstMBI GATC 2 cut(s) 956, 970
BstMWI GCNNNNNNNGC 6 cut(s) 90, 239, 254, 330, 357, 472
BstNI CCWGG 2 cut(s) 269, 534
BstSCI CCNGG 4 cut(s) 42, 43, 267, 532
BstSLI GKGCMC 1 cut(s) 994
BstUI CGCG 1 cut(s) 454
BstV1I GCAGC 2 cut(s) 266, 953
BstX2I RGATCY 1 cut(s) 956
BstYI RGATCY 1 cut(s) 956
BsuRI GGCC 7 cut(s) 129, 167, 300, 324, 333, 451, 755
BtgI CCRYGG 1 cut(s) 859
BtrI CACGTC 1 cut(s) 142
BtsCI GGATG 5 cut(s) 95, 168, 306, 493, 750
BtsIMutI CAGTG 4 cut(s) 168, 726, 837, 998
BveI ACCTGC 1 cut(s) 1003
Cac8I GCNNGC 3 cut(s) 82, 716, 835
CaiI CAGNNNCTG 1 cut(s) 425
CfoI GCGC 3 cut(s) 456, 466, 811
Cfr10I RCCGGY 1 cut(s) 755
Cfr13I GGNCC 7 cut(s) 47, 128, 299, 449, 537, 679, 922
Cfr9I CCCGGG 1 cut(s) 43
CseI GACGC 1 cut(s) 440
Csp6I GTAC 2 cut(s) 896, 914
CviAII CATG 2 cut(s) 397, 412
CviQI GTAC 2 cut(s) 896, 914
DdeI CTNAG 2 cut(s) 541, 821
DpnI GATC 2 cut(s) 958, 972
DpnII GATC 2 cut(s) 956, 970
EaeI YGGCCR 1 cut(s) 165
EciI GGCGGA 1 cut(s) 220
Eco130I CCWWGG 3 cut(s) 355, 388, 682
Eco31I GGTCTC 2 cut(s) 369, 628
Eco47I GGWCC 4 cut(s) 47, 537, 679, 922
Eco57I CTGAAG 3 cut(s) 861, 966, 1022
Eco88I CYCGRG 2 cut(s) 43, 301
EcoO109I RGGNCCY 4 cut(s) 47, 128, 537, 679
EcoRII CCWGG 2 cut(s) 267, 532
EcoT14I CCWWGG 3 cut(s) 355, 388, 682
EcoT22I ATGCAT 1 cut(s) 1069
ErhI CCWWGG 3 cut(s) 355, 388, 682
FaeI CATG 2 cut(s) 400, 415
FalI AAGNNNNNCTT 2 cut(s) 464, 496
FaqI GGGAC 1 cut(s) 550
FatI CATG 2 cut(s) 396, 411
FauI CCCGC 1 cut(s) 974
FbaI TGATCA 1 cut(s) 970
FblI GTMKAC 2 cut(s) 277, 1056
Fnu4HI GCNGC 3 cut(s) 255, 452, 942
FokI GGATG 5 cut(s) 102, 175, 293, 500, 757
Fsp4HI GCNGC 3 cut(s) 255, 452, 942
FspBI CTAG 4 cut(s) 251, 326, 515, 1044
GlaI GCGC 3 cut(s) 455, 465, 810
GluI GCNGC 3 cut(s) 255, 452, 942
GsaI CCCAGC 1 cut(s) 931
HaeII RGCGCY 1 cut(s) 812
HaeIII GGCC 7 cut(s) 129, 167, 300, 324, 333, 451, 755
HapII CCGG 3 cut(s) 44, 756, 812
HgaI GACGC 1 cut(s) 440
HhaI GCGC 3 cut(s) 456, 466, 811
Hin1I GRCGYC 1 cut(s) 771
Hin1II CATG 2 cut(s) 400, 415
Hin6I GCGC 3 cut(s) 454, 464, 809
HinP1I GCGC 3 cut(s) 454, 464, 809
HinfI GANTC 4 cut(s) 59, 97, 380, 408
HpaII CCGG 3 cut(s) 44, 756, 812
HphI GGTGA 5 cut(s) 23, 512, 598, 770, 997
Hpy166II GTNNAC 3 cut(s) 278, 992, 1057
Hpy188I TCNGA 6 cut(s) 58, 76, 544, 642, 798, 975
Hpy188III TCNNGA 5 cut(s) 20, 198, 419, 481, 1044
Hpy8I GTNNAC 3 cut(s) 278, 992, 1057
HpyAV CCTTC 4 cut(s) 80, 323, 904, 944
HpyCH4III ACNGT 3 cut(s) 11, 172, 593
HpyCH4IV ACGT 2 cut(s) 141, 771
HpyF10VI GCNNNNNNNGC 6 cut(s) 90, 239, 254, 330, 357, 472
HpyF3I CTNAG 2 cut(s) 541, 821
HpySE526I ACGT 2 cut(s) 141, 771
Hsp92I GRCGYC 1 cut(s) 771
Hsp92II CATG 2 cut(s) 400, 415
HspAI GCGC 3 cut(s) 454, 464, 809
KpnI GGTACC 2 cut(s) 899, 917
Ksp22I TGATCA 1 cut(s) 970
Kzo9I GATC 2 cut(s) 956, 970
LmnI GCTCC 4 cut(s) 124, 532, 790, 866
Lsp1109I GCAGC 2 cut(s) 266, 953
LweI GCATC 7 cut(s) 67, 80, 306, 315, 514, 565, 802
MaeI CTAG 4 cut(s) 251, 326, 515, 1044
MaeII ACGT 2 cut(s) 141, 771
MaeIII GTNAC 2 cut(s) 604, 772
MalI GATC 2 cut(s) 958, 972
MboI GATC 2 cut(s) 956, 970
MboII GAAGA 2 cut(s) 959, 1015
MfeI CAATTG 3 cut(s) 150, 402, 558
MflI RGATCY 1 cut(s) 956
MhlI GDGCHC 3 cut(s) 224, 552, 994
MlsI TGGCCA 1 cut(s) 167
MluCI AATT 8 cut(s) 15, 105, 150, 402, 476, 558, 734, 815
MluNI TGGCCA 1 cut(s) 167
MlyI GAGTC 1 cut(s) 389
MmeI TCCRAC 2 cut(s) 408, 649
MnlI CCTC 7 cut(s) 60, 438, 477, 540, 550, 735, 745
Mox20I TGGCCA 1 cut(s) 167
Mph1103I ATGCAT 1 cut(s) 1069
MscI TGGCCA 1 cut(s) 167
MseI TTAA 1 cut(s) 471
MslI CAYNNNNRTG 1 cut(s) 842
Msp20I TGGCCA 1 cut(s) 167
MspA1I CMGCKG 1 cut(s) 944
MspI CCGG 3 cut(s) 44, 756, 812
MspR9I CCNGG 4 cut(s) 44, 45, 269, 534
MunI CAATTG 3 cut(s) 150, 402, 558
MvaI CCWGG 2 cut(s) 269, 534
MvnI CGCG 1 cut(s) 454
MwoI GCNNNNNNNGC 6 cut(s) 90, 239, 254, 330, 357, 472
NciI CCSGG 2 cut(s) 44, 45
NdeII GATC 2 cut(s) 956, 970
NlaIII CATG 2 cut(s) 400, 415
NlaIV GGNNCC 6 cut(s) 48, 450, 538, 897, 915, 924
NmuCI GTSAC 2 cut(s) 604, 772
NsiI ATGCAT 1 cut(s) 1069
OliI CACNNNNGTG 1 cut(s) 842
PaqCI CACCTGC 1 cut(s) 1003
PfeI GAWTC 3 cut(s) 59, 97, 408
PkrI GCNGC 3 cut(s) 256, 453, 943
PleI GAGTC 1 cut(s) 388
PpsI GAGTC 1 cut(s) 388
PpuMI RGGWCCY 3 cut(s) 47, 537, 679
Psp5II RGGWCCY 3 cut(s) 47, 537, 679
Psp6I CCWGG 2 cut(s) 267, 532
PspFI CCCAGC 1 cut(s) 927
PspGI CCWGG 2 cut(s) 267, 532
PspN4I GGNNCC 6 cut(s) 48, 450, 538, 897, 915, 924
PspPI GGNCC 7 cut(s) 47, 128, 299, 449, 537, 679, 922
PspPPI RGGWCCY 3 cut(s) 47, 537, 679
PstNI CAGNNNCTG 1 cut(s) 425
PsuI RGATCY 1 cut(s) 956
PvuII CAGCTG 1 cut(s) 944
RsaI GTAC 2 cut(s) 897, 915
RsaNI GTAC 2 cut(s) 896, 914
RseI CAYNNNNRTG 1 cut(s) 842
SaqAI TTAA 1 cut(s) 471
SatI GCNGC 3 cut(s) 255, 452, 942
Sau3AI GATC 2 cut(s) 956, 970
Sau96I GGNCC 7 cut(s) 47, 128, 299, 449, 537, 679, 922
SchI GAGTC 1 cut(s) 389
ScrFI CCNGG 4 cut(s) 44, 45, 269, 534
SduI GDGCHC 3 cut(s) 224, 552, 994
SfaNI GCATC 7 cut(s) 67, 80, 306, 315, 514, 565, 802
SfiI GGCCNNNNNGGCC 1 cut(s) 330
SinI GGWCC 4 cut(s) 47, 537, 679, 922
SmaI CCCGGG 1 cut(s) 45
SmiMI CAYNNNNRTG 1 cut(s) 842
SmlI CTYRAG 1 cut(s) 481
SmoI CTYRAG 1 cut(s) 481
Sse9I AATT 8 cut(s) 15, 105, 150, 402, 476, 558, 734, 815
SsiI CCGC 3 cut(s) 231, 452, 981
SspMI CTAG 4 cut(s) 251, 326, 515, 1044
StyD4I CCNGG 4 cut(s) 42, 43, 267, 532
StyI CCWWGG 3 cut(s) 355, 388, 682
TaaI ACNGT 3 cut(s) 11, 172, 593
TaiI ACGT 2 cut(s) 144, 774
TasI AATT 8 cut(s) 15, 105, 150, 402, 476, 558, 734, 815
TauI GCSGC 1 cut(s) 454
TfiI GAWTC 3 cut(s) 59, 97, 408
Tru1I TTAA 1 cut(s) 471
Tru9I TTAA 1 cut(s) 471
TscAI CASTG 4 cut(s) 175, 726, 844, 1005
TseFI GTSAC 2 cut(s) 604, 772
TseI GCWGC 2 cut(s) 254, 941
Tsp45I GTSAC 2 cut(s) 604, 772
TspDTI ATGAA 2 cut(s) 48, 400
TspMI CCCGGG 1 cut(s) 43
TspRI CASTG 4 cut(s) 175, 726, 844, 1005
VneI GTGCAC 1 cut(s) 990
VpaK11BI GGWCC 4 cut(s) 47, 537, 679, 922
XapI RAATTY 4 cut(s) 15, 105, 734, 815
XbaI TCTAGA 1 cut(s) 1043
XcmI CCANNNNNNNNNTGG 1 cut(s) 353
XmaI CCCGGG 1 cut(s) 43
XmiI GTMKAC 2 cut(s) 277, 1056
XspI CTAG 4 cut(s) 251, 326, 515, 1044
ZraI GACGTC 1 cut(s) 772
Zsp2I ATGCAT 1 cut(s) 1069
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.