RchiOBHm_Chr2g0174341

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. Functions as Fe-S scaffold, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Essential for embryo development

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_chinensis
2
OG0012080
Physical Location & Seq
Forward (+)
86963506 .. 86965899
2394 bp
Loading structure...
UTR
Exon/CDS
Intron
PRQ54150

Sequence Viewer

Length: 1074 bp
ATGGAGAACGGCGAAATACCCGAAAACGCTAATGAACATTGCCCAGGTCCTCAATCCGATTCTGCTGGGAAGTCTGAATCTTGTGAAGGATGCCCGAATCAACAAATTTGTGCTACTGCTCCAAAGGGCCCTGACCCAGACTTGGTTGCAATTGCAGAAAGGATGGCCACTGTAAAGCATAAGATACTGATTTTATCAGGGAAAGGTGGAGTTGGCAAGAGCACATTCTCTGCACAACTTTCATTTGCACTAGCGGCTATGGACTTCCAGGTGGGTCTTCTGGACATCGACATTTGTGGCCCTAGCATCCCAAAGATGCTTGGCCTAGAAGGCCAAGAAATCCACCAGAGCAACCTCGGTTGGTCTCCTGTCTATGTTGAGTCCAACCTTGGGGTTATGTCAATAGGGTTTATGCTTCCAGACCCTGATGACGCAGTCATATGGAGGGGGCCACGCAAGAATGCACTCATTAAGCAATTCCTCAAGGATGTTGATTGGGGTGAGCTTGATTTTCTAATAGTTGATGCTCCTCCTGGAACCTCAGATGAACACATTTCAATTGTTCAAGATCTTGGTGCTGCTTCAATAGATGGTGCAATCATTGTCACCACTCCACAACAAGTTTCCCTTATTGATGTCAGGAAAGAAGTGAGTTTCTGCAAAAAAGTTGGAGTCCAGGTTCTTGGGGTAGTTGAAAATATGAGTGGCTTGCGCCAGCCGATGTTGGCTGCTTCCAAATTTTTGAGGATGACAGAGGCAGGTGAGGAGAAAGATGTCACAGAATGGGTTAGAGAGTACGTGAAAGAGAAAGCACCGGAATTGCTTGATATAATAGCCTTGAATGAAGTGTTTGATAATAGTGGTGGTGGAGCCACAAGAATGTGCAGTGAAATGGGGGTACCTTTCCTGGGGAAGGTGCCGTTGGATCCCGAGCTTTGCAAGGCTGCTGAAGAAGGTAGATCTTGTTTTGTTGATCAGAAAAGTGGCACGAGTGCACCTGCACTGCAGAAAATCATCGAGACATTGATGGAAAATCAAGGCATATCTAGAATGTTGGTAGACAATGATGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0042802 GO:0042803 GO:0044424 GO:0044444 GO:0044464 GO:0046983 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ParA

Protein Analysis

357

Amino Acids

38.33

Weight (kDa)

4.59

Isoelectric Point (pI)

36.17

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ParA PF10609 58 - 243 2e-80 NUBPL iron-transfer P-loop NTPase
AAA_31 PF13614 61 - 213 2.6e-06 AAA domain
CbiA PF01656 62 - 320 1.2e-12 CobQ/CobB/MinD/ParA nucleotide binding domain
ParA PF10609 282 - 342 3.8e-07 NUBPL iron-transfer P-loop NTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50960
fragaria_vesca FvH4_6g52730
malus_domestica MD09G1017000.v1.1 MD17G1017500.v1.1
prunus_persica Prupe.3G300400_v2.0.a1 Prupe.3G300400_v2.0.a1
pyrus_communis pycom111g01380 pycom17g01410
rosa_chinensis RchiOBHm_Chr2g0174341
rosa_laevigata RLG00000022259
rosa_multiflora Rmu_sc0003649.1_g000032 Rmu_sc0007150.1_g000002
rosa_roxburghii Rroxscaffold_2G00078280
rosa_rugosa Rorug02G0576400
rosa_samantha Rh2AG657300 Rh2BG668300 Rh2CG631400 Rh2DG681900
rosa_wichuraiana Rw2G053830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 2 cut(s) 749, 1006
Acc36I ACCTGC 2 cut(s) 749, 1006
Acc65I GGTACC 1 cut(s) 898
AccB1I GGYRCC 2 cut(s) 898, 916
AccI GTMKAC 1 cut(s) 1059
AciI CCGC 1 cut(s) 254
AclWI GGATC 2 cut(s) 920, 933
AcoI YGGCCR 1 cut(s) 165
AcsI RAATTY 2 cut(s) 105, 737
AcuI CTGAAG 1 cut(s) 969
AfaI GTAC 2 cut(s) 797, 900
AfiI CCNNNNNNNGG 4 cut(s) 142, 390, 908, 913
AgsI TTSAA 5 cut(s) 558, 566, 585, 695, 841
AjnI CCWGG 5 cut(s) 43, 267, 532, 675, 906
AjuI GAANNNNNNNTTGG 2 cut(s) 905, 937
AloI GAACNNNNNNTCC 2 cut(s) 663, 695
AluBI AGCT 2 cut(s) 505, 934
AluI AGCT 2 cut(s) 505, 934
Alw21I GWGCWC 2 cut(s) 224, 997
Alw26I GTCTC 2 cut(s) 369, 1013
Alw44I GTGCAC 1 cut(s) 993
AlwI GGATC 2 cut(s) 920, 933
AlwNI CAGNNNCTG 1 cut(s) 425
Ama87I CYCGRG 1 cut(s) 929
AoxI GGCC 6 cut(s) 127, 165, 298, 322, 331, 449
ApaI GGGCCC 1 cut(s) 131
ApaLI GTGCAC 1 cut(s) 993
ApeKI GCWGC 3 cut(s) 578, 728, 944
ApoI RAATTY 2 cut(s) 105, 737
Asp718I GGTACC 1 cut(s) 898
AspLEI GCGC 1 cut(s) 714
AspS9I GGNCC 5 cut(s) 47, 127, 128, 299, 449
AsuHPI GGTGA 3 cut(s) 512, 598, 773
AvaI CYCGRG 1 cut(s) 929
AvaII GGWCC 1 cut(s) 47
BaeGI GKGCMC 2 cut(s) 131, 997
BalI TGGCCA 1 cut(s) 167
BamHI GGATCC 1 cut(s) 925
BanI GGYRCC 2 cut(s) 898, 916
BanII GRGCYC 1 cut(s) 131
BauI CACGAG 1 cut(s) 988
BbsI GAAGAC 1 cut(s) 269
Bbv12I GWGCWC 2 cut(s) 224, 997
BbvI GCAGC 3 cut(s) 565, 715, 931
BccI CCATC 3 cut(s) 157, 584, 1021
BceAI ACGGC 2 cut(s) 25, 904
BciT130I CCWGG 5 cut(s) 45, 269, 534, 677, 908
BclI TGATCA 1 cut(s) 973
BcoDI GTCTC 2 cut(s) 369, 1013
BfaI CTAG 4 cut(s) 251, 303, 326, 1047
BfmI CTRYAG 1 cut(s) 1004
BfuAI ACCTGC 2 cut(s) 749, 1006
BglI GCCNNNNNGGC 1 cut(s) 330
BglII AGATCT 2 cut(s) 568, 959
BisI GCNGC 4 cut(s) 255, 579, 729, 945
BlsI GCNGC 4 cut(s) 256, 580, 730, 946
Bme1390I CCNGG 5 cut(s) 45, 269, 534, 677, 908
Bme18I GGWCC 1 cut(s) 47
BmeT110I CYCGRG 1 cut(s) 929
BmgT120I GGNCC 5 cut(s) 47, 127, 128, 299, 449
BmiI GGNNCC 7 cut(s) 129, 450, 538, 871, 900, 918, 927
BmrFI CCNGG 5 cut(s) 45, 269, 534, 677, 908
BmsI GCATC 5 cut(s) 80, 306, 315, 514, 1057
BpiI GAAGAC 1 cut(s) 269
BpuEI CTTGAG 1 cut(s) 467
BsaAI YACGTR 1 cut(s) 799
BsaI GGTCTC 1 cut(s) 369
BsaJI CCNNGG 4 cut(s) 43, 355, 388, 907
BsaWI WCCGGW 1 cut(s) 814
BsaXI ACNNNNNCTCC 2 cut(s) 663, 693
Bsc4I CCNNNNNNNGG 4 cut(s) 142, 390, 908, 913
Bse3DI GCAATG 1 cut(s) 37
BseBI CCWGG 5 cut(s) 45, 269, 534, 677, 908
BseDI CCNNGG 4 cut(s) 43, 355, 388, 907
BseGI GGATG 5 cut(s) 95, 168, 306, 493, 753
BseLI CCNNNNNNNGG 4 cut(s) 142, 390, 908, 913
BseMI GCAATG 1 cut(s) 37
BseMII CTCAG 1 cut(s) 555
BseRI GAGGAG 2 cut(s) 519, 779
BseSI GKGCMC 2 cut(s) 131, 997
BseXI GCAGC 3 cut(s) 565, 715, 931
BseYI CCCAGC 1 cut(s) 65
BsgI GTGCAG 3 cut(s) 216, 904, 984
BshFI GGCC 6 cut(s) 129, 167, 300, 324, 333, 451
BshNI GGYRCC 2 cut(s) 898, 916
BsiHKAI GWGCWC 2 cut(s) 224, 997
BsiHKCI CYCGRG 1 cut(s) 929
BsiSI CCGG 1 cut(s) 815
BslI CCNNNNNNNGG 4 cut(s) 142, 390, 908, 913
BsmAI GTCTC 2 cut(s) 369, 1013
BsmI GAATGC 1 cut(s) 466
BsnI GGCC 6 cut(s) 129, 167, 300, 324, 333, 451
Bso31I GGTCTC 1 cut(s) 369
BsoBI CYCGRG 1 cut(s) 929
Bsp120I GGGCCC 1 cut(s) 127
Bsp1286I GDGCHC 3 cut(s) 131, 224, 997
Bsp143I GATC 4 cut(s) 568, 925, 959, 973
BspACI CCGC 1 cut(s) 254
BspANI GGCC 6 cut(s) 129, 167, 300, 324, 333, 451
BspCNI CTCAG 1 cut(s) 554
BspLI GGNNCC 7 cut(s) 129, 450, 538, 871, 900, 918, 927
BspMAI CTGCAG 1 cut(s) 1008
BspMI ACCTGC 2 cut(s) 749, 1006
BspPI GGATC 2 cut(s) 920, 933
BspT107I GGYRCC 2 cut(s) 898, 916
BspTNI GGTCTC 1 cut(s) 369
BsrDI GCAATG 1 cut(s) 37
BssECI CCNNGG 4 cut(s) 43, 355, 388, 907
BssMI GATC 4 cut(s) 568, 925, 959, 973
BssSI CACGAG 1 cut(s) 988
BssT1I CCWWGG 1 cut(s) 388
Bst2BI CACGAG 1 cut(s) 988
Bst2UI CCWGG 5 cut(s) 45, 269, 534, 677, 908
Bst4CI ACNGT 1 cut(s) 172
BstBAI YACGTR 1 cut(s) 799
BstC8I GCNNGC 2 cut(s) 710, 716
BstDEI CTNAG 1 cut(s) 541
BstENI CCTNNNNNAGG 1 cut(s) 911
BstF5I GGATG 5 cut(s) 95, 168, 306, 493, 753
BstHHI GCGC 1 cut(s) 714
BstKTI GATC 4 cut(s) 571, 928, 962, 976
BstMAI GTCTC 2 cut(s) 369, 1013
BstMBI GATC 4 cut(s) 568, 925, 959, 973
BstMWI GCNNNNNNNGC 2 cut(s) 254, 330
BstNI CCWGG 5 cut(s) 45, 269, 534, 677, 908
BstSCI CCNGG 5 cut(s) 43, 267, 532, 675, 906
BstSFI CTRYAG 1 cut(s) 1004
BstSLI GKGCMC 2 cut(s) 131, 997
BstV1I GCAGC 3 cut(s) 565, 715, 931
BstV2I GAAGAC 1 cut(s) 269
BstX2I RGATCY 3 cut(s) 568, 925, 959
BstXI CCANNNNNNTGG 1 cut(s) 683
BstYI RGATCY 3 cut(s) 568, 925, 959
BsuRI GGCC 6 cut(s) 129, 167, 300, 324, 333, 451
BtsCI GGATG 5 cut(s) 95, 168, 306, 493, 753
BtsI GCAGTG 2 cut(s) 892, 1001
BtsIMutI CAGTG 3 cut(s) 168, 892, 1001
BveI ACCTGC 2 cut(s) 749, 1006
Cac8I GCNNGC 2 cut(s) 710, 716
CaiI CAGNNNCTG 1 cut(s) 425
CfoI GCGC 1 cut(s) 714
Cfr13I GGNCC 5 cut(s) 47, 127, 128, 299, 449
CseI GACGC 1 cut(s) 440
Csp6I GTAC 2 cut(s) 796, 899
CviQI GTAC 2 cut(s) 796, 899
DdeI CTNAG 1 cut(s) 541
DpnI GATC 4 cut(s) 570, 927, 961, 975
DpnII GATC 4 cut(s) 568, 925, 959, 973
EaeI YGGCCR 1 cut(s) 165
Eco130I CCWWGG 1 cut(s) 388
Eco24I GRGCYC 1 cut(s) 131
Eco31I GGTCTC 1 cut(s) 369
Eco47I GGWCC 1 cut(s) 47
Eco57I CTGAAG 1 cut(s) 969
Eco88I CYCGRG 1 cut(s) 929
EcoNI CCTNNNNNAGG 1 cut(s) 911
EcoO109I RGGNCCY 3 cut(s) 47, 127, 128
EcoRII CCWGG 5 cut(s) 43, 267, 532, 675, 906
EcoT14I CCWWGG 1 cut(s) 388
EcoT22I ATGCAT 1 cut(s) 1072
EcoT38I GRGCYC 1 cut(s) 131
ErhI CCWWGG 1 cut(s) 388
FalI AAGNNNNNCTT 2 cut(s) 612, 644
FauNDI CATATG 1 cut(s) 440
FbaI TGATCA 1 cut(s) 973
FblI GTMKAC 1 cut(s) 1059
Fnu4HI GCNGC 4 cut(s) 255, 579, 729, 945
FokI GGATG 5 cut(s) 102, 175, 293, 500, 760
FriOI GRGCYC 1 cut(s) 131
Fsp4HI GCNGC 4 cut(s) 255, 579, 729, 945
FspBI CTAG 4 cut(s) 251, 303, 326, 1047
GlaI GCGC 1 cut(s) 713
GluI GCNGC 4 cut(s) 255, 579, 729, 945
GsaI CCCAGC 1 cut(s) 69
HaeIII GGCC 6 cut(s) 129, 167, 300, 324, 333, 451
HapII CCGG 1 cut(s) 815
HgaI GACGC 1 cut(s) 440
HhaI GCGC 1 cut(s) 714
Hin6I GCGC 1 cut(s) 712
HinP1I GCGC 1 cut(s) 712
HinfI GANTC 5 cut(s) 59, 77, 97, 380, 672
HpaII CCGG 1 cut(s) 815
HphI GGTGA 3 cut(s) 512, 598, 773
Hpy166II GTNNAC 2 cut(s) 995, 1060
Hpy188I TCNGA 4 cut(s) 58, 76, 544, 978
Hpy188III TCNNGA 7 cut(s) 281, 419, 566, 640, 929, 1018, 1047
Hpy8I GTNNAC 2 cut(s) 995, 1060
HpyAV CCTTC 4 cut(s) 80, 323, 907, 947
HpyCH4III ACNGT 1 cut(s) 172
HpyCH4IV ACGT 1 cut(s) 798
HpyF10VI GCNNNNNNNGC 2 cut(s) 254, 330
HpyF3I CTNAG 1 cut(s) 541
HpySE526I ACGT 1 cut(s) 798
HspAI GCGC 1 cut(s) 712
KpnI GGTACC 1 cut(s) 902
Ksp22I TGATCA 1 cut(s) 973
Kzo9I GATC 4 cut(s) 568, 925, 959, 973
LmnI GCTCC 3 cut(s) 124, 532, 869
Lsp1109I GCAGC 3 cut(s) 565, 715, 931
LweI GCATC 5 cut(s) 80, 306, 315, 514, 1057
MaeI CTAG 4 cut(s) 251, 303, 326, 1047
MaeII ACGT 1 cut(s) 798
MaeIII GTNAC 2 cut(s) 604, 775
MalI GATC 4 cut(s) 570, 927, 961, 975
MboI GATC 4 cut(s) 568, 925, 959, 973
MboII GAAGA 2 cut(s) 269, 962
MfeI CAATTG 2 cut(s) 150, 558
MflI RGATCY 3 cut(s) 568, 925, 959
MhlI GDGCHC 3 cut(s) 131, 224, 997
MlsI TGGCCA 1 cut(s) 167
MluCI AATT 6 cut(s) 105, 150, 476, 558, 737, 818
MluNI TGGCCA 1 cut(s) 167
MlyI GAGTC 2 cut(s) 389, 681
MmeI TCCRAC 3 cut(s) 408, 649, 903
MnlI CCTC 9 cut(s) 60, 365, 438, 491, 540, 550, 738, 748, 757
Mox20I TGGCCA 1 cut(s) 167
Mph1103I ATGCAT 1 cut(s) 1072
MscI TGGCCA 1 cut(s) 167
MseI TTAA 1 cut(s) 471
MslI CAYNNNNRTG 1 cut(s) 878
Msp20I TGGCCA 1 cut(s) 167
MspI CCGG 1 cut(s) 815
MspR9I CCNGG 5 cut(s) 45, 269, 534, 677, 908
MunI CAATTG 2 cut(s) 150, 558
Mva1269I GAATGC 1 cut(s) 466
MvaI CCWGG 5 cut(s) 45, 269, 534, 677, 908
MwoI GCNNNNNNNGC 2 cut(s) 254, 330
NdeI CATATG 1 cut(s) 440
NdeII GATC 4 cut(s) 568, 925, 959, 973
NlaIV GGNNCC 7 cut(s) 129, 450, 538, 871, 900, 918, 927
NmuCI GTSAC 2 cut(s) 604, 775
NsiI ATGCAT 1 cut(s) 1072
PaqCI CACCTGC 2 cut(s) 749, 1006
PctI GAATGC 1 cut(s) 466
PfeI GAWTC 3 cut(s) 59, 77, 97
PflFI GACNNNGTC 1 cut(s) 434
PfoI TCCNGGA 1 cut(s) 532
PkrI GCNGC 4 cut(s) 256, 580, 730, 946
PleI GAGTC 2 cut(s) 388, 680
PpsI GAGTC 2 cut(s) 388, 680
Ppu21I YACGTR 1 cut(s) 799
PpuMI RGGWCCY 1 cut(s) 47
Psp5II RGGWCCY 1 cut(s) 47
Psp6I CCWGG 5 cut(s) 43, 267, 532, 675, 906
PspFI CCCAGC 1 cut(s) 65
PspGI CCWGG 5 cut(s) 43, 267, 532, 675, 906
PspN4I GGNNCC 7 cut(s) 129, 450, 538, 871, 900, 918, 927
PspOMI GGGCCC 1 cut(s) 127
PspPI GGNCC 5 cut(s) 47, 127, 128, 299, 449
PspPPI RGGWCCY 1 cut(s) 47
PstI CTGCAG 1 cut(s) 1008
PstNI CAGNNNCTG 1 cut(s) 425
PsuI RGATCY 3 cut(s) 568, 925, 959
PsyI GACNNNGTC 1 cut(s) 434
RsaI GTAC 2 cut(s) 797, 900
RsaNI GTAC 2 cut(s) 796, 899
RseI CAYNNNNRTG 1 cut(s) 878
SaqAI TTAA 1 cut(s) 471
SatI GCNGC 4 cut(s) 255, 579, 729, 945
Sau3AI GATC 4 cut(s) 568, 925, 959, 973
Sau96I GGNCC 5 cut(s) 47, 127, 128, 299, 449
SchI GAGTC 2 cut(s) 389, 681
ScrFI CCNGG 5 cut(s) 45, 269, 534, 677, 908
SduI GDGCHC 3 cut(s) 131, 224, 997
SfaNI GCATC 5 cut(s) 80, 306, 315, 514, 1057
SfcI CTRYAG 1 cut(s) 1004
SfiI GGCCNNNNNGGCC 1 cut(s) 330
SinI GGWCC 1 cut(s) 47
SmiMI CAYNNNNRTG 1 cut(s) 878
SmlI CTYRAG 1 cut(s) 482
SmoI CTYRAG 1 cut(s) 482
Sse9I AATT 6 cut(s) 105, 150, 476, 558, 737, 818
SsiI CCGC 1 cut(s) 254
SspMI CTAG 4 cut(s) 251, 303, 326, 1047
StyD4I CCNGG 5 cut(s) 43, 267, 532, 675, 906
StyI CCWWGG 1 cut(s) 388
TaaI ACNGT 1 cut(s) 172
TaiI ACGT 1 cut(s) 801
TaqI TCGA 2 cut(s) 288, 1017
TasI AATT 6 cut(s) 105, 150, 476, 558, 737, 818
TauI GCSGC 1 cut(s) 257
TfiI GAWTC 3 cut(s) 59, 77, 97
Tru1I TTAA 1 cut(s) 471
Tru9I TTAA 1 cut(s) 471
TscAI CASTG 3 cut(s) 175, 892, 1008
TseFI GTSAC 2 cut(s) 604, 775
TseI GCWGC 3 cut(s) 578, 728, 944
Tsp45I GTSAC 2 cut(s) 604, 775
TspDTI ATGAA 4 cut(s) 48, 231, 561, 858
TspRI CASTG 3 cut(s) 175, 892, 1008
Tth111I GACNNNGTC 1 cut(s) 434
VneI GTGCAC 1 cut(s) 993
VpaK11BI GGWCC 1 cut(s) 47
XagI CCTNNNNNAGG 1 cut(s) 911
XapI RAATTY 2 cut(s) 105, 737
XbaI TCTAGA 1 cut(s) 1046
XmiI GTMKAC 1 cut(s) 1059
XspI CTAG 4 cut(s) 251, 303, 326, 1047
Zsp2I ATGCAT 1 cut(s) 1072
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.