pycom17g01410

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. Functions as Fe-S scaffold, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Essential for embryo development

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr17
OG0012080
Physical Location & Seq
Reverse (-)
956173 .. 958018
1846 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom17g01410.2

Sequence Viewer

Length: 834 bp
ATGGAGAACGGTGAAATTCCCGAAGACGCCAACGAACATTGCCCGGGTCCGCAGTCCGAATCGGCTGGAAAATCCGACGCCTGCGAAGGATGCCCGAACCAGCAGATTTGTGCTACTGCTCCCAAGGGCCCCGACCCAGACGTGGTTGCAATTGCAGAAAGGATGGCCACTGTAAAGCATAAGATACTGGTTTTATCAGGAAAAGGTGGAGTTGGCAAGAGCACATTCTCTGCCCAACTTGCATTTGCACTAGCAGCTATGGACTTCCAGGTGGGACTTCTTGATATCGATATTTGTGGCCCGAGCATCCCAAAGATGCTCGGCCTAGAAGGCCAAGAAATCCACCAGAGCAACCTTGGCTGGTCTCCTGTCTATGTTGAGCAAAACCTCGGGGTCATGTCAATTGGGTTTATGCTTCCAGATCCTGATGACGCTGTTATTTGGCGGGGGCCGCGTAAAAATGCGCTCATCAAGCAATTCTTGAAGGATGTTTATTGGGGTGAGCTTGATTTCCTGATAGTGGATGCTCCTCCTGGAACCTCGGACGAGCATATTTCAATTGTCCAATACCTTGATGCTGGCTCAATAGATGGTGCAATCATAGTCACCACTCCACAACAGGTCTCCCTGATTGATGTTCGGAAAGAAGTAAGTTTCTGCAAGAAAGTTGGAGTTCATGTCCTTGGGGTTGTTGAAAACATGAGTGGTTTGTCCCAGCCATTGATGGATTTCAAATTTTTGAGGATGACAGAGACCGGTGAGCAAGTAGACGTCACAGAATGGGCTAGGGAGCAGCTGAAAGAGAGATCGATCCAGAATTTTCAAACATTGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0042802 GO:0042803 GO:0044424 GO:0044444 GO:0044464 GO:0046983 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ParA

Protein Analysis

278

Amino Acids

30.09

Weight (kDa)

4.65

Isoelectric Point (pI)

29.75

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50960
fragaria_vesca FvH4_6g52730
malus_domestica MD09G1017000.v1.1 MD17G1017500.v1.1
prunus_persica Prupe.3G300400_v2.0.a1 Prupe.3G300400_v2.0.a1
pyrus_communis pycom111g01380 pycom17g01410
rosa_chinensis RchiOBHm_Chr2g0174341
rosa_laevigata RLG00000022259
rosa_multiflora Rmu_sc0003649.1_g000032 Rmu_sc0007150.1_g000002
rosa_roxburghii Rroxscaffold_2G00078280
rosa_rugosa Rorug02G0576400
rosa_samantha Rh2AG657300 Rh2BG668300 Rh2CG631400 Rh2DG681900
rosa_wichuraiana Rw2G053830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 774
AccI GTMKAC 1 cut(s) 768
AccII CGCG 1 cut(s) 454
AciI CCGC 3 cut(s) 50, 445, 452
AclWI GGATC 2 cut(s) 416, 805
AcoI YGGCCR 1 cut(s) 165
AcsI RAATTY 3 cut(s) 15, 734, 817
AcyI GRCGYC 3 cut(s) 27, 78, 771
AfiI CCNNNNNNNGG 2 cut(s) 142, 520
AgeI ACCGGT 1 cut(s) 755
AgsI TTSAA 5 cut(s) 484, 558, 695, 733, 824
AjiI CACGTC 1 cut(s) 142
AjnI CCWGG 2 cut(s) 267, 532
AluBI AGCT 3 cut(s) 257, 505, 796
AluI AGCT 3 cut(s) 257, 505, 796
Alw21I GWGCWC 1 cut(s) 224
Alw26I GTCTC 3 cut(s) 369, 628, 746
AlwI GGATC 2 cut(s) 416, 805
AlwNI CAGNNNCTG 1 cut(s) 425
Ama87I CYCGRG 3 cut(s) 43, 301, 389
AoxI GGCC 6 cut(s) 127, 165, 298, 322, 331, 449
ApaI GGGCCC 1 cut(s) 131
ApeKI GCWGC 2 cut(s) 254, 793
ApoI RAATTY 3 cut(s) 15, 734, 817
AsiGI ACCGGT 1 cut(s) 755
AspLEI GCGC 1 cut(s) 466
AspS9I GGNCC 5 cut(s) 47, 127, 128, 299, 449
AsuC2I CCSGG 2 cut(s) 44, 45
AsuHPI GGTGA 4 cut(s) 23, 512, 598, 770
AvaI CYCGRG 3 cut(s) 43, 301, 389
AvaII GGWCC 1 cut(s) 47
BaeGI GKGCMC 1 cut(s) 131
BalI TGGCCA 1 cut(s) 167
BanII GRGCYC 1 cut(s) 131
BbsI GAAGAC 1 cut(s) 30
Bbv12I GWGCWC 1 cut(s) 224
BbvI GCAGC 2 cut(s) 266, 805
BccI CCATC 3 cut(s) 157, 584, 718
BciT130I CCWGG 2 cut(s) 269, 534
BcnI CCSGG 2 cut(s) 44, 45
BcoDI GTCTC 3 cut(s) 369, 628, 746
BfaI CTAG 3 cut(s) 251, 326, 786
BglI GCCNNNNNGGC 1 cut(s) 330
BisI GCNGC 3 cut(s) 255, 452, 794
BlsI GCNGC 3 cut(s) 256, 453, 795
Bme1390I CCNGG 4 cut(s) 44, 45, 269, 534
Bme18I GGWCC 1 cut(s) 47
BmeT110I CYCGRG 3 cut(s) 43, 301, 389
BmgBI CACGTC 1 cut(s) 142
BmgT120I GGNCC 5 cut(s) 47, 127, 128, 299, 449
BmiI GGNNCC 5 cut(s) 48, 129, 130, 450, 538
BmrFI CCNGG 4 cut(s) 44, 45, 269, 534
BmsI GCATC 5 cut(s) 80, 306, 315, 514, 565
BpiI GAAGAC 1 cut(s) 30
BpuMI CCSGG 2 cut(s) 44, 45
Bsa29I ATCGAT 2 cut(s) 288, 809
BsaHI GRCGYC 3 cut(s) 27, 78, 771
BsaI GGTCTC 3 cut(s) 369, 628, 746
BsaJI CCNNGG 6 cut(s) 43, 123, 355, 388, 540, 682
BsaWI WCCGGW 1 cut(s) 755
BsaXI ACNNNNNCTCC 2 cut(s) 663, 693
Bsc4I CCNNNNNNNGG 2 cut(s) 142, 520
Bse118I RCCGGY 1 cut(s) 755
Bse1I ACTGG 1 cut(s) 192
Bse3DI GCAATG 1 cut(s) 37
BseBI CCWGG 2 cut(s) 269, 534
BseCI ATCGAT 2 cut(s) 288, 809
BseDI CCNNGG 6 cut(s) 43, 123, 355, 388, 540, 682
BseGI GGATG 6 cut(s) 95, 168, 306, 493, 529, 750
BseLI CCNNNNNNNGG 2 cut(s) 142, 520
BseMI GCAATG 1 cut(s) 37
BseNI ACTGG 1 cut(s) 192
BseRI GAGGAG 1 cut(s) 519
BseSI GKGCMC 1 cut(s) 131
BseXI GCAGC 2 cut(s) 266, 805
BseYI CCCAGC 1 cut(s) 714
Bsh1236I CGCG 1 cut(s) 454
BshFI GGCC 6 cut(s) 129, 167, 300, 324, 333, 451
BshTI ACCGGT 1 cut(s) 755
BshVI ATCGAT 2 cut(s) 288, 809
BsiHKAI GWGCWC 1 cut(s) 224
BsiHKCI CYCGRG 3 cut(s) 43, 301, 389
BsiSI CCGG 2 cut(s) 44, 756
BslFI GGGAC 2 cut(s) 288, 697
BslI CCNNNNNNNGG 2 cut(s) 142, 520
BsmAI GTCTC 3 cut(s) 369, 628, 746
BsmFI GGGAC 2 cut(s) 288, 697
BsnI GGCC 6 cut(s) 129, 167, 300, 324, 333, 451
Bso31I GGTCTC 3 cut(s) 369, 628, 746
BsoBI CYCGRG 3 cut(s) 43, 301, 389
Bsp120I GGGCCC 1 cut(s) 127
Bsp1286I GDGCHC 2 cut(s) 131, 224
Bsp143I GATC 3 cut(s) 421, 806, 810
BspACI CCGC 3 cut(s) 50, 445, 452
BspANI GGCC 6 cut(s) 129, 167, 300, 324, 333, 451
BspDI ATCGAT 2 cut(s) 288, 809
BspFNI CGCG 1 cut(s) 454
BspLI GGNNCC 5 cut(s) 48, 129, 130, 450, 538
BspPI GGATC 2 cut(s) 416, 805
BspTNI GGTCTC 3 cut(s) 369, 628, 746
BsrDI GCAATG 1 cut(s) 37
BsrFI RCCGGY 1 cut(s) 755
BsrI ACTGG 1 cut(s) 192
BssAI RCCGGY 1 cut(s) 755
BssECI CCNNGG 6 cut(s) 43, 123, 355, 388, 540, 682
BssMI GATC 3 cut(s) 421, 806, 810
BssNI GRCGYC 3 cut(s) 27, 78, 771
BssT1I CCWWGG 3 cut(s) 123, 355, 682
Bst2UI CCWGG 2 cut(s) 269, 534
Bst4CI ACNGT 2 cut(s) 11, 172
BstACI GRCGYC 3 cut(s) 27, 78, 771
BstC8I GCNNGC 2 cut(s) 82, 580
BstF5I GGATG 6 cut(s) 95, 168, 306, 493, 529, 750
BstFNI CGCG 1 cut(s) 454
BstHHI GCGC 1 cut(s) 466
BstKTI GATC 3 cut(s) 424, 809, 813
BstMAI GTCTC 3 cut(s) 369, 628, 746
BstMBI GATC 3 cut(s) 421, 806, 810
BstMWI GCNNNNNNNGC 7 cut(s) 90, 239, 254, 330, 357, 451, 472
BstNI CCWGG 2 cut(s) 269, 534
BstSCI CCNGG 4 cut(s) 42, 43, 267, 532
BstSLI GKGCMC 1 cut(s) 131
BstUI CGCG 1 cut(s) 454
BstV1I GCAGC 2 cut(s) 266, 805
BstV2I GAAGAC 1 cut(s) 30
BstX2I RGATCY 1 cut(s) 421
BstYI RGATCY 1 cut(s) 421
Bsu15I ATCGAT 2 cut(s) 288, 809
BsuRI GGCC 6 cut(s) 129, 167, 300, 324, 333, 451
BsuTUI ATCGAT 2 cut(s) 288, 809
BtrI CACGTC 1 cut(s) 142
BtsCI GGATG 6 cut(s) 95, 168, 306, 493, 529, 750
BtsIMutI CAGTG 1 cut(s) 168
Cac8I GCNNGC 2 cut(s) 82, 580
CaiI CAGNNNCTG 1 cut(s) 425
CfoI GCGC 1 cut(s) 466
Cfr10I RCCGGY 1 cut(s) 755
Cfr13I GGNCC 5 cut(s) 47, 127, 128, 299, 449
Cfr9I CCCGGG 1 cut(s) 43
ClaI ATCGAT 2 cut(s) 288, 809
CseI GACGC 3 cut(s) 35, 86, 440
CspAI ACCGGT 1 cut(s) 755
CviAII CATG 3 cut(s) 397, 677, 700
DpnI GATC 3 cut(s) 423, 808, 812
DpnII GATC 3 cut(s) 421, 806, 810
EaeI YGGCCR 1 cut(s) 165
Eco130I CCWWGG 3 cut(s) 123, 355, 682
Eco24I GRGCYC 1 cut(s) 131
Eco31I GGTCTC 3 cut(s) 369, 628, 746
Eco32I GATATC 1 cut(s) 286
Eco47I GGWCC 1 cut(s) 47
Eco88I CYCGRG 3 cut(s) 43, 301, 389
EcoO109I RGGNCCY 2 cut(s) 127, 128
EcoRII CCWGG 2 cut(s) 267, 532
EcoRV GATATC 1 cut(s) 286
EcoT14I CCWWGG 3 cut(s) 123, 355, 682
EcoT38I GRGCYC 1 cut(s) 131
ErhI CCWWGG 3 cut(s) 123, 355, 682
FaeI CATG 3 cut(s) 400, 680, 703
FaiI YATR 9 cut(s) 180, 260, 375, 398, 413, 552, 602, 678, 701
FalI AAGNNNNNCTT 2 cut(s) 464, 496
FaqI GGGAC 2 cut(s) 288, 697
FatI CATG 3 cut(s) 396, 676, 699
FauI CCCGC 1 cut(s) 438
FblI GTMKAC 1 cut(s) 768
Fnu4HI GCNGC 3 cut(s) 255, 452, 794
FokI GGATG 6 cut(s) 102, 175, 293, 500, 536, 757
FriOI GRGCYC 1 cut(s) 131
Fsp4HI GCNGC 3 cut(s) 255, 452, 794
FspBI CTAG 3 cut(s) 251, 326, 786
GlaI GCGC 1 cut(s) 465
GluI GCNGC 3 cut(s) 255, 452, 794
GsaI CCCAGC 1 cut(s) 718
HaeIII GGCC 6 cut(s) 129, 167, 300, 324, 333, 451
HapII CCGG 2 cut(s) 44, 756
HgaI GACGC 3 cut(s) 35, 86, 440
HhaI GCGC 1 cut(s) 466
Hin1I GRCGYC 3 cut(s) 27, 78, 771
Hin1II CATG 3 cut(s) 400, 680, 703
Hin6I GCGC 1 cut(s) 464
HinP1I GCGC 1 cut(s) 464
HinfI GANTC 1 cut(s) 59
HpaII CCGG 2 cut(s) 44, 756
HphI GGTGA 4 cut(s) 23, 512, 598, 770
Hpy166II GTNNAC 1 cut(s) 769
Hpy188I TCNGA 4 cut(s) 58, 76, 544, 642
Hpy188III TCNNGA 8 cut(s) 20, 198, 281, 419, 425, 481, 514, 814
Hpy8I GTNNAC 1 cut(s) 769
Hpy99I CGWCG 1 cut(s) 80
HpyAV CCTTC 3 cut(s) 80, 323, 478
HpyCH4III ACNGT 2 cut(s) 11, 172
HpyCH4IV ACGT 2 cut(s) 141, 771
HpyCH4V TGCA 6 cut(s) 149, 155, 242, 248, 596, 660
HpyF10VI GCNNNNNNNGC 7 cut(s) 90, 239, 254, 330, 357, 451, 472
HpySE526I ACGT 2 cut(s) 141, 771
Hsp92I GRCGYC 3 cut(s) 27, 78, 771
Hsp92II CATG 3 cut(s) 400, 680, 703
HspAI GCGC 1 cut(s) 464
Kzo9I GATC 3 cut(s) 421, 806, 810
LmnI GCTCC 3 cut(s) 124, 532, 790
Lsp1109I GCAGC 2 cut(s) 266, 805
LweI GCATC 5 cut(s) 80, 306, 315, 514, 565
MaeI CTAG 3 cut(s) 251, 326, 786
MaeII ACGT 2 cut(s) 141, 771
MaeIII GTNAC 2 cut(s) 604, 772
MalI GATC 3 cut(s) 423, 808, 812
MboI GATC 3 cut(s) 421, 806, 810
MboII GAAGA 1 cut(s) 35
MfeI CAATTG 3 cut(s) 150, 402, 558
MflI RGATCY 1 cut(s) 421
MhlI GDGCHC 2 cut(s) 131, 224
MlsI TGGCCA 1 cut(s) 167
MluCI AATT 7 cut(s) 15, 150, 402, 476, 558, 734, 817
MluNI TGGCCA 1 cut(s) 167
MmeI TCCRAC 2 cut(s) 99, 649
MnlI CCTC 4 cut(s) 398, 540, 550, 735
Mox20I TGGCCA 1 cut(s) 167
MscI TGGCCA 1 cut(s) 167
Msp20I TGGCCA 1 cut(s) 167
MspA1I CMGCKG 1 cut(s) 796
MspI CCGG 2 cut(s) 44, 756
MspR9I CCNGG 4 cut(s) 44, 45, 269, 534
MunI CAATTG 3 cut(s) 150, 402, 558
MvaI CCWGG 2 cut(s) 269, 534
MvnI CGCG 1 cut(s) 454
MwoI GCNNNNNNNGC 7 cut(s) 90, 239, 254, 330, 357, 451, 472
NciI CCSGG 2 cut(s) 44, 45
NdeII GATC 3 cut(s) 421, 806, 810
NlaIII CATG 3 cut(s) 400, 680, 703
NlaIV GGNNCC 5 cut(s) 48, 129, 130, 450, 538
NmeAIII GCCGAG 1 cut(s) 300
NmuCI GTSAC 2 cut(s) 604, 772
PfeI GAWTC 1 cut(s) 59
PfoI TCCNGGA 1 cut(s) 532
PinAI ACCGGT 1 cut(s) 755
PkrI GCNGC 3 cut(s) 256, 453, 795
Psp6I CCWGG 2 cut(s) 267, 532
PspFI CCCAGC 1 cut(s) 714
PspGI CCWGG 2 cut(s) 267, 532
PspN4I GGNNCC 5 cut(s) 48, 129, 130, 450, 538
PspOMI GGGCCC 1 cut(s) 127
PspPI GGNCC 5 cut(s) 47, 127, 128, 299, 449
PstNI CAGNNNCTG 1 cut(s) 425
PsuI RGATCY 1 cut(s) 421
PvuII CAGCTG 1 cut(s) 796
SatI GCNGC 3 cut(s) 255, 452, 794
Sau3AI GATC 3 cut(s) 421, 806, 810
Sau96I GGNCC 5 cut(s) 47, 127, 128, 299, 449
ScrFI CCNGG 4 cut(s) 44, 45, 269, 534
SduI GDGCHC 2 cut(s) 131, 224
SfaNI GCATC 5 cut(s) 80, 306, 315, 514, 565
SfiI GGCCNNNNNGGCC 1 cut(s) 330
SinI GGWCC 1 cut(s) 47
SmaI CCCGGG 1 cut(s) 45
Sse9I AATT 7 cut(s) 15, 150, 402, 476, 558, 734, 817
SsiI CCGC 3 cut(s) 50, 445, 452
SspMI CTAG 3 cut(s) 251, 326, 786
StyD4I CCNGG 4 cut(s) 42, 43, 267, 532
StyI CCWWGG 3 cut(s) 123, 355, 682
TaaI ACNGT 2 cut(s) 11, 172
TaiI ACGT 2 cut(s) 144, 774
TaqI TCGA 2 cut(s) 288, 809
TasI AATT 7 cut(s) 15, 150, 402, 476, 558, 734, 817
TauI GCSGC 1 cut(s) 454
TfiI GAWTC 1 cut(s) 59
TscAI CASTG 1 cut(s) 175
TseFI GTSAC 2 cut(s) 604, 772
TseI GCWGC 2 cut(s) 254, 793
Tsp45I GTSAC 2 cut(s) 604, 772
TspDTI ATGAA 1 cut(s) 665
TspMI CCCGGG 1 cut(s) 43
TspRI CASTG 1 cut(s) 175
VpaK11BI GGWCC 1 cut(s) 47
XapI RAATTY 3 cut(s) 15, 734, 817
XcmI CCANNNNNNNNNTGG 1 cut(s) 353
XmaI CCCGGG 1 cut(s) 43
XmiI GTMKAC 1 cut(s) 768
XspI CTAG 3 cut(s) 251, 326, 786
ZraI GACGTC 1 cut(s) 772
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.