Rh2AG657300

Component of the cytosolic iron-sulfur (Fe-S) protein assembly (CIA) machinery. Required for maturation of extramitochondrial Fe-S proteins. Functions as Fe-S scaffold, mediating the de novo assembly of an Fe-S cluster and its transfer to target apoproteins. Essential for embryo development

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr2A
OG0012080
Physical Location & Seq
Forward (+)
87345588 .. 87348267
2680 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh2AG657300.1

Sequence Viewer

Length: 912 bp
ATGGCCACTGTAAAGCATAAGATACTGATTTTATCAGGGAAAGGTGGAGTTGGCAAGAGCACATTCTCTGCACAACTTTCATTTGCACTAGCGGCTATGGACTTCCAGGTGGGTCTTCTGGACATCGACATTTGTGGCCCTAGCATCCCAAAGATGCTTGGCCTAGAAGGCCAAGAAATCCACCAGAGCAACCTCGGTTGGTCTCCTGTCTATGTTGAGTCCAACCTTGGGGTTATGTCAATAGGGTTTATGCTTCCAGACCCTGATGACGCAGTCATATGGAGGGGGCCACGCAAGAATGCACTCATTAAGCAATTCCTCAAAGATGTTGATTGGGGTGAGCTTGATTTTCTAATAGTTGATGCTCCTCCTGGAACCTCAGATGAACACATTTCAATTGTTCAAGATCTTGGTGCTGCTTCAATAGATGGTGCAATCATTGTCACCACTCCACAACAAGTTTCCCTTATTGATGTCAGGAAAGAAGTGAGCTTCTGCAAAAAAGTTGGAGTCCAGGTTCTTGGGGTAGTTGAAAATATGAGTGGCTTGCGCCAGCCGATGTTGGCTGCTTCCAAATTTTTGAGGATGACAGAGGCGGGTGAGGAGAAAGATGTCACAGAATGGGTTAGAGAGTACGTGAAAGAGAAAGCACCGGAATTGCTTGATATAATAGCCTTGAATGAAGTGTTTGATAATAGTGGTGGTGGAGCCACAAGAATGTGCAGTGAAATGGGGGTACCTTTCCTGGGGAAGGTGCCGTTGGATCCCGAGCTTTGCAAGGCTGCTGAAGAAGGTAGATCTTGTTTTGTTGATCAGAAAAGTGGCACGAGTGCACCTGCACTGCAGAAAATCATCGAGACATTGATGGAAAATCAAGGCATATCTAGAATGTTAGTAGACAATGATGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0005488 GO:0005515 GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005829 GO:0042802 GO:0042803 GO:0044424 GO:0044444 GO:0044464 GO:0046983 GO:0048037 GO:0051536 GO:0051540
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
ParA

Protein Analysis

303

Amino Acids

32.75

Weight (kDa)

4.72

Isoelectric Point (pI)

34.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
ParA PF10609 4 - 189 1.3e-80 NUBPL iron-transfer P-loop NTPase
AAA_31 PF13614 7 - 159 1.7e-06 AAA domain
CbiA PF01656 8 - 266 6.9e-13 CobQ/CobB/MinD/ParA nucleotide binding domain
ParA PF10609 228 - 288 2.9e-07 NUBPL iron-transfer P-loop NTPase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012080)

Species Orthologous Gene IDs
arabidopsis_thaliana AT5G50960
fragaria_vesca FvH4_6g52730
malus_domestica MD09G1017000.v1.1 MD17G1017500.v1.1
prunus_persica Prupe.3G300400_v2.0.a1 Prupe.3G300400_v2.0.a1
pyrus_communis pycom111g01380 pycom17g01410
rosa_chinensis RchiOBHm_Chr2g0174341
rosa_laevigata RLG00000022259
rosa_multiflora Rmu_sc0003649.1_g000032 Rmu_sc0007150.1_g000002
rosa_roxburghii Rroxscaffold_2G00078280
rosa_rugosa Rorug02G0576400
rosa_samantha Rh2AG657300 Rh2BG668300 Rh2CG631400 Rh2DG681900
rosa_wichuraiana Rw2G053830

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 844
Acc36I ACCTGC 1 cut(s) 844
Acc65I GGTACC 1 cut(s) 736
AccB1I GGYRCC 2 cut(s) 736, 754
AccI GTMKAC 1 cut(s) 897
AciI CCGC 2 cut(s) 92, 596
AclWI GGATC 2 cut(s) 758, 771
AcoI YGGCCR 1 cut(s) 3
AcsI RAATTY 1 cut(s) 575
AcuI CTGAAG 1 cut(s) 807
AfaI GTAC 2 cut(s) 635, 738
AfiI CCNNNNNNNGG 3 cut(s) 228, 746, 751
AgsI TTSAA 5 cut(s) 396, 404, 423, 533, 679
AjnI CCWGG 4 cut(s) 105, 370, 513, 744
AjuI GAANNNNNNNTTGG 2 cut(s) 743, 775
AloI GAACNNNNNNTCC 2 cut(s) 501, 533
AluBI AGCT 3 cut(s) 343, 492, 772
AluI AGCT 3 cut(s) 343, 492, 772
Alw21I GWGCWC 2 cut(s) 62, 835
Alw26I GTCTC 2 cut(s) 207, 851
Alw44I GTGCAC 1 cut(s) 831
AlwI GGATC 2 cut(s) 758, 771
AlwNI CAGNNNCTG 1 cut(s) 263
Ama87I CYCGRG 1 cut(s) 767
AoxI GGCC 5 cut(s) 3, 136, 160, 169, 287
ApaLI GTGCAC 1 cut(s) 831
ApeKI GCWGC 3 cut(s) 416, 566, 782
ApoI RAATTY 1 cut(s) 575
Asp718I GGTACC 1 cut(s) 736
AspLEI GCGC 1 cut(s) 552
AspS9I GGNCC 2 cut(s) 137, 287
AsuHPI GGTGA 3 cut(s) 350, 436, 611
AvaI CYCGRG 1 cut(s) 767
BaeGI GKGCMC 1 cut(s) 835
BalI TGGCCA 1 cut(s) 5
BamHI GGATCC 1 cut(s) 763
BanI GGYRCC 2 cut(s) 736, 754
BauI CACGAG 1 cut(s) 826
BbsI GAAGAC 1 cut(s) 107
Bbv12I GWGCWC 2 cut(s) 62, 835
BbvI GCAGC 3 cut(s) 403, 553, 769
BccI CCATC 2 cut(s) 422, 859
BceAI ACGGC 1 cut(s) 742
BciT130I CCWGG 4 cut(s) 107, 372, 515, 746
BclI TGATCA 1 cut(s) 811
BcoDI GTCTC 2 cut(s) 207, 851
BfaI CTAG 4 cut(s) 89, 141, 164, 885
BfmI CTRYAG 1 cut(s) 842
BfuAI ACCTGC 1 cut(s) 844
BglI GCCNNNNNGGC 1 cut(s) 168
BglII AGATCT 2 cut(s) 406, 797
BisI GCNGC 4 cut(s) 93, 417, 567, 783
BlsI GCNGC 4 cut(s) 94, 418, 568, 784
Bme1390I CCNGG 4 cut(s) 107, 372, 515, 746
BmeT110I CYCGRG 1 cut(s) 767
BmgT120I GGNCC 2 cut(s) 137, 287
BmiI GGNNCC 6 cut(s) 288, 376, 709, 738, 756, 765
BmrFI CCNGG 4 cut(s) 107, 372, 515, 746
BmsI GCATC 4 cut(s) 144, 153, 352, 895
BpiI GAAGAC 1 cut(s) 107
BsaAI YACGTR 1 cut(s) 637
BsaI GGTCTC 1 cut(s) 207
BsaJI CCNNGG 3 cut(s) 193, 226, 745
BsaWI WCCGGW 1 cut(s) 652
BsaXI ACNNNNNCTCC 2 cut(s) 501, 531
Bsc4I CCNNNNNNNGG 3 cut(s) 228, 746, 751
BseBI CCWGG 4 cut(s) 107, 372, 515, 746
BseDI CCNNGG 3 cut(s) 193, 226, 745
BseGI GGATG 2 cut(s) 144, 591
BseLI CCNNNNNNNGG 3 cut(s) 228, 746, 751
BseMII CTCAG 1 cut(s) 393
BseRI GAGGAG 2 cut(s) 357, 617
BseSI GKGCMC 1 cut(s) 835
BseXI GCAGC 3 cut(s) 403, 553, 769
BsgI GTGCAG 3 cut(s) 54, 742, 822
BshFI GGCC 5 cut(s) 5, 138, 162, 171, 289
BshNI GGYRCC 2 cut(s) 736, 754
BsiHKAI GWGCWC 2 cut(s) 62, 835
BsiHKCI CYCGRG 1 cut(s) 767
BsiSI CCGG 1 cut(s) 653
BslI CCNNNNNNNGG 3 cut(s) 228, 746, 751
BsmAI GTCTC 2 cut(s) 207, 851
BsmI GAATGC 1 cut(s) 304
BsnI GGCC 5 cut(s) 5, 138, 162, 171, 289
Bso31I GGTCTC 1 cut(s) 207
BsoBI CYCGRG 1 cut(s) 767
Bsp1286I GDGCHC 2 cut(s) 62, 835
Bsp143I GATC 4 cut(s) 406, 763, 797, 811
BspACI CCGC 2 cut(s) 92, 596
BspANI GGCC 5 cut(s) 5, 138, 162, 171, 289
BspCNI CTCAG 1 cut(s) 392
BspLI GGNNCC 6 cut(s) 288, 376, 709, 738, 756, 765
BspMAI CTGCAG 1 cut(s) 846
BspMI ACCTGC 1 cut(s) 844
BspPI GGATC 2 cut(s) 758, 771
BspT107I GGYRCC 2 cut(s) 736, 754
BspTNI GGTCTC 1 cut(s) 207
BssECI CCNNGG 3 cut(s) 193, 226, 745
BssMI GATC 4 cut(s) 406, 763, 797, 811
BssSI CACGAG 1 cut(s) 826
BssT1I CCWWGG 1 cut(s) 226
Bst2BI CACGAG 1 cut(s) 826
Bst2UI CCWGG 4 cut(s) 107, 372, 515, 746
Bst4CI ACNGT 1 cut(s) 10
BstBAI YACGTR 1 cut(s) 637
BstC8I GCNNGC 2 cut(s) 548, 554
BstDEI CTNAG 1 cut(s) 379
BstENI CCTNNNNNAGG 1 cut(s) 749
BstF5I GGATG 2 cut(s) 144, 591
BstHHI GCGC 1 cut(s) 552
BstKTI GATC 4 cut(s) 409, 766, 800, 814
BstMAI GTCTC 2 cut(s) 207, 851
BstMBI GATC 4 cut(s) 406, 763, 797, 811
BstMWI GCNNNNNNNGC 2 cut(s) 92, 168
BstNI CCWGG 4 cut(s) 107, 372, 515, 746
BstSCI CCNGG 4 cut(s) 105, 370, 513, 744
BstSFI CTRYAG 1 cut(s) 842
BstSLI GKGCMC 1 cut(s) 835
BstV1I GCAGC 3 cut(s) 403, 553, 769
BstV2I GAAGAC 1 cut(s) 107
BstX2I RGATCY 3 cut(s) 406, 763, 797
BstXI CCANNNNNNTGG 1 cut(s) 521
BstYI RGATCY 3 cut(s) 406, 763, 797
BsuRI GGCC 5 cut(s) 5, 138, 162, 171, 289
BtsCI GGATG 2 cut(s) 144, 591
BtsI GCAGTG 2 cut(s) 730, 839
BtsIMutI CAGTG 3 cut(s) 6, 730, 839
BveI ACCTGC 1 cut(s) 844
Cac8I GCNNGC 2 cut(s) 548, 554
CaiI CAGNNNCTG 1 cut(s) 263
CfoI GCGC 1 cut(s) 552
Cfr13I GGNCC 2 cut(s) 137, 287
CseI GACGC 1 cut(s) 278
Csp6I GTAC 2 cut(s) 634, 737
CviQI GTAC 2 cut(s) 634, 737
DdeI CTNAG 1 cut(s) 379
DpnI GATC 4 cut(s) 408, 765, 799, 813
DpnII GATC 4 cut(s) 406, 763, 797, 811
EaeI YGGCCR 1 cut(s) 3
Eco130I CCWWGG 1 cut(s) 226
Eco31I GGTCTC 1 cut(s) 207
Eco57I CTGAAG 1 cut(s) 807
Eco88I CYCGRG 1 cut(s) 767
EcoNI CCTNNNNNAGG 1 cut(s) 749
EcoRII CCWGG 4 cut(s) 105, 370, 513, 744
EcoT14I CCWWGG 1 cut(s) 226
EcoT22I ATGCAT 1 cut(s) 910
ErhI CCWWGG 1 cut(s) 226
FalI AAGNNNNNCTT 2 cut(s) 450, 482
FauI CCCGC 1 cut(s) 589
FauNDI CATATG 1 cut(s) 278
FbaI TGATCA 1 cut(s) 811
FblI GTMKAC 1 cut(s) 897
Fnu4HI GCNGC 4 cut(s) 93, 417, 567, 783
FokI GGATG 2 cut(s) 131, 598
Fsp4HI GCNGC 4 cut(s) 93, 417, 567, 783
FspBI CTAG 4 cut(s) 89, 141, 164, 885
GlaI GCGC 1 cut(s) 551
GluI GCNGC 4 cut(s) 93, 417, 567, 783
HaeIII GGCC 5 cut(s) 5, 138, 162, 171, 289
HapII CCGG 1 cut(s) 653
HgaI GACGC 1 cut(s) 278
HhaI GCGC 1 cut(s) 552
Hin6I GCGC 1 cut(s) 550
HinP1I GCGC 1 cut(s) 550
HinfI GANTC 2 cut(s) 218, 510
HpaII CCGG 1 cut(s) 653
HphI GGTGA 3 cut(s) 350, 436, 611
Hpy166II GTNNAC 2 cut(s) 833, 898
Hpy188I TCNGA 2 cut(s) 382, 816
Hpy188III TCNNGA 7 cut(s) 119, 257, 404, 478, 767, 856, 885
Hpy8I GTNNAC 2 cut(s) 833, 898
HpyAV CCTTC 3 cut(s) 161, 745, 785
HpyCH4III ACNGT 1 cut(s) 10
HpyCH4IV ACGT 1 cut(s) 636
HpyF10VI GCNNNNNNNGC 2 cut(s) 92, 168
HpyF3I CTNAG 1 cut(s) 379
HpySE526I ACGT 1 cut(s) 636
HspAI GCGC 1 cut(s) 550
KpnI GGTACC 1 cut(s) 740
Ksp22I TGATCA 1 cut(s) 811
Kzo9I GATC 4 cut(s) 406, 763, 797, 811
LmnI GCTCC 2 cut(s) 370, 707
Lsp1109I GCAGC 3 cut(s) 403, 553, 769
LweI GCATC 4 cut(s) 144, 153, 352, 895
MaeI CTAG 4 cut(s) 89, 141, 164, 885
MaeII ACGT 1 cut(s) 636
MaeIII GTNAC 2 cut(s) 442, 613
MalI GATC 4 cut(s) 408, 765, 799, 813
MboI GATC 4 cut(s) 406, 763, 797, 811
MboII GAAGA 2 cut(s) 107, 800
MfeI CAATTG 1 cut(s) 396
MflI RGATCY 3 cut(s) 406, 763, 797
MhlI GDGCHC 2 cut(s) 62, 835
MlsI TGGCCA 1 cut(s) 5
MluCI AATT 4 cut(s) 314, 396, 575, 656
MluNI TGGCCA 1 cut(s) 5
MlyI GAGTC 2 cut(s) 227, 519
MmeI TCCRAC 3 cut(s) 246, 487, 741
MnlI CCTC 8 cut(s) 203, 276, 329, 378, 388, 576, 586, 595
Mox20I TGGCCA 1 cut(s) 5
Mph1103I ATGCAT 1 cut(s) 910
MscI TGGCCA 1 cut(s) 5
MseI TTAA 1 cut(s) 309
MslI CAYNNNNRTG 1 cut(s) 716
Msp20I TGGCCA 1 cut(s) 5
MspI CCGG 1 cut(s) 653
MspR9I CCNGG 4 cut(s) 107, 372, 515, 746
MunI CAATTG 1 cut(s) 396
Mva1269I GAATGC 1 cut(s) 304
MvaI CCWGG 4 cut(s) 107, 372, 515, 746
MwoI GCNNNNNNNGC 2 cut(s) 92, 168
NdeI CATATG 1 cut(s) 278
NdeII GATC 4 cut(s) 406, 763, 797, 811
NlaIV GGNNCC 6 cut(s) 288, 376, 709, 738, 756, 765
NmuCI GTSAC 2 cut(s) 442, 613
NsiI ATGCAT 1 cut(s) 910
PaqCI CACCTGC 1 cut(s) 844
PctI GAATGC 1 cut(s) 304
PflFI GACNNNGTC 1 cut(s) 272
PfoI TCCNGGA 1 cut(s) 370
PkrI GCNGC 4 cut(s) 94, 418, 568, 784
PleI GAGTC 2 cut(s) 226, 518
PpsI GAGTC 2 cut(s) 226, 518
Ppu21I YACGTR 1 cut(s) 637
Psp6I CCWGG 4 cut(s) 105, 370, 513, 744
PspGI CCWGG 4 cut(s) 105, 370, 513, 744
PspN4I GGNNCC 6 cut(s) 288, 376, 709, 738, 756, 765
PspPI GGNCC 2 cut(s) 137, 287
PstI CTGCAG 1 cut(s) 846
PstNI CAGNNNCTG 1 cut(s) 263
PsuI RGATCY 3 cut(s) 406, 763, 797
PsyI GACNNNGTC 1 cut(s) 272
RsaI GTAC 2 cut(s) 635, 738
RsaNI GTAC 2 cut(s) 634, 737
RseI CAYNNNNRTG 1 cut(s) 716
SaqAI TTAA 1 cut(s) 309
SatI GCNGC 4 cut(s) 93, 417, 567, 783
Sau3AI GATC 4 cut(s) 406, 763, 797, 811
Sau96I GGNCC 2 cut(s) 137, 287
SchI GAGTC 2 cut(s) 227, 519
ScrFI CCNGG 4 cut(s) 107, 372, 515, 746
SduI GDGCHC 2 cut(s) 62, 835
SfaNI GCATC 4 cut(s) 144, 153, 352, 895
SfcI CTRYAG 1 cut(s) 842
SfiI GGCCNNNNNGGCC 1 cut(s) 168
SmiMI CAYNNNNRTG 1 cut(s) 716
Sse9I AATT 4 cut(s) 314, 396, 575, 656
SsiI CCGC 2 cut(s) 92, 596
SspMI CTAG 4 cut(s) 89, 141, 164, 885
StyD4I CCNGG 4 cut(s) 105, 370, 513, 744
StyI CCWWGG 1 cut(s) 226
TaaI ACNGT 1 cut(s) 10
TaiI ACGT 1 cut(s) 639
TaqI TCGA 2 cut(s) 126, 855
TasI AATT 4 cut(s) 314, 396, 575, 656
TauI GCSGC 1 cut(s) 95
Tru1I TTAA 1 cut(s) 309
Tru9I TTAA 1 cut(s) 309
TscAI CASTG 3 cut(s) 13, 730, 846
TseFI GTSAC 2 cut(s) 442, 613
TseI GCWGC 3 cut(s) 416, 566, 782
Tsp45I GTSAC 2 cut(s) 442, 613
TspDTI ATGAA 3 cut(s) 69, 399, 696
TspRI CASTG 3 cut(s) 13, 730, 846
Tth111I GACNNNGTC 1 cut(s) 272
VneI GTGCAC 1 cut(s) 831
XagI CCTNNNNNAGG 1 cut(s) 749
XapI RAATTY 1 cut(s) 575
XbaI TCTAGA 1 cut(s) 884
XmiI GTMKAC 1 cut(s) 897
XspI CTAG 4 cut(s) 89, 141, 164, 885
Zsp2I ATGCAT 1 cut(s) 910
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.