AT5G52020

ethylene-responsive transcription factor

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0012077
Physical Location & Seq
Reverse (-)
21123958 .. 21125065
1108 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G52020.1

Sequence Viewer

Length: 699 bp
ATGTCGAATAATAATAATTCTCCGACCACCGTGAATCAAGAAACGACGACGTCTCGTGAAGTCTCAATCACATTGCCTACTGATCAATCTCCTCAAACCTCACCAGGATCATCTTCTTCTCCTTCACCGAGACCTTCCGGTGGATCACCGGCGAGAAGAACGGCGACTGGATTATCCGGCAAGCACTCTATTTTCAGGGGGATTCGACTACGTAACGGAAAATGGGTATCGGAGATTAGAGAGCCACGTAAAACGACAAGAATTTGGCTCGGGACTTATCCGGTACCGGAGATGGCTGCCGCCGCTTACGACGTGGCTGCGTTAGCTTTAAAAGGACCCGACGCCGTTTTGAATTTTCCTGGTTTAGCTTTGACTTACGTGGCTCCGGTTTCAAACTCTGCTGCGGATATAAGAGCGGCTGCTAGTAGAGCAGCGGAGATGAAGCAACCGGATCAGGGTGGGGATGAGAAGGTATTGGAACCGGTTCAACCCGGCAAAGAGGAAGAATTAGAAGAAGTGTCGTGTAACTCGTGTTCGTTGGAGTTTATGGATGAGGAAGCGATGTTGAATATGCCGACTTTGTTGACGGAGATGGCTGAAGGGATGTTGATGAGTCCACCGAGAATGATGATACATCCGACGATGGAAGATGATTCGCCGGAGAATCATGAAGGAGATAATCTTTGGAGTTATAAATGA

Protein Analysis

232

Amino Acids

25.11

Weight (kDa)

4.86

Isoelectric Point (pI)

68.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
AP2 PF00847 65 - 112 5.3e-10 AP2 domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Orthologous Genes (Group: OG0012077)

Species Orthologous Gene IDs
arabidopsis_thaliana AT1G12630 AT5G52020
fragaria_vesca FvH4_7g28960
malus_domestica MD01G1196300.v1.1 MD07G1263200.v1.1
prunus_persica Prupe.2G289600_v2.0.a1
pyrus_communis pycom01g20680 pycom07g24220
rosa_chinensis RchiOBHm_Chr1g0376651
rosa_laevigata RLG00000026584
rosa_multiflora Rmu_co8365147.1_g000001 Rmu_sc0005594.1_g000003
rosa_roxburghii Rroxscaffold_4G00281910
rosa_rugosa Rorug01G0397100
rosa_samantha Rh1AG414000 Rh1BG373400 Rh1CG387300 Rh1DG404400
rosa_wichuraiana Rw1G036320

Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 693
AatII GACGTC 1 cut(s) 53
Acc65I GGTACC 1 cut(s) 283
AccB1I GGYRCC 1 cut(s) 283
AccBSI CCGCTC 1 cut(s) 416
AciI CCGC 5 cut(s) 300, 303, 404, 416, 434
AclWI GGATC 3 cut(s) 115, 151, 459
AcsI RAATTY 2 cut(s) 261, 352
AcuI CTGAAG 1 cut(s) 618
AcyI GRCGYC 2 cut(s) 50, 342
AfaI GTAC 1 cut(s) 285
AfiI CCNNNNNNNGG 2 cut(s) 140, 455
AgeI ACCGGT 1 cut(s) 481
AgsI TTSAA 4 cut(s) 352, 393, 488, 568
AjiI CACGTC 1 cut(s) 313
AjnI CCWGG 2 cut(s) 103, 358
AluBI AGCT 2 cut(s) 326, 368
AluI AGCT 2 cut(s) 326, 368
Alw26I GTCTC 3 cut(s) 57, 67, 124
AlwI GGATC 3 cut(s) 115, 151, 459
Ama87I CYCGRG 1 cut(s) 269
ApeKI GCWGC 5 cut(s) 296, 317, 401, 419, 431
ApoI RAATTY 2 cut(s) 261, 352
AsiGI ACCGGT 1 cut(s) 481
Asp700I GAANNNNTTC 1 cut(s) 483
Asp718I GGTACC 1 cut(s) 283
AspS9I GGNCC 1 cut(s) 335
AsuC2I CCSGG 1 cut(s) 492
AsuHPI GGTGA 3 cut(s) 93, 117, 138
AvaI CYCGRG 1 cut(s) 269
AvaII GGWCC 1 cut(s) 335
BanI GGYRCC 1 cut(s) 283
BauI CACGAG 2 cut(s) 54, 529
BbvI GCAGC 5 cut(s) 283, 304, 388, 406, 443
BccI CCATC 3 cut(s) 286, 586, 637
BceAI ACGGC 2 cut(s) 177, 329
BcgI CGANNNNNNTGC 2 cut(s) 299, 333
BciT130I CCWGG 2 cut(s) 105, 360
BclI TGATCA 1 cut(s) 82
BcnI CCSGG 1 cut(s) 492
BcoDI GTCTC 3 cut(s) 57, 67, 124
BfaI CTAG 1 cut(s) 423
BisI GCNGC 8 cut(s) 297, 300, 303, 318, 402, 417, 420, 432
BlsI GCNGC 8 cut(s) 298, 301, 304, 319, 403, 418, 421, 433
Bme1390I CCNGG 3 cut(s) 105, 360, 492
Bme18I GGWCC 1 cut(s) 335
BmeT110I CYCGRG 1 cut(s) 269
BmgBI CACGTC 1 cut(s) 313
BmgT120I GGNCC 1 cut(s) 335
BmiI GGNNCC 4 cut(s) 285, 337, 384, 480
BmrFI CCNGG 3 cut(s) 105, 360, 492
BpuMI CCSGG 1 cut(s) 492
BsaAI YACGTR 3 cut(s) 212, 248, 379
BsaHI GRCGYC 2 cut(s) 50, 342
BsaI GGTCTC 1 cut(s) 124
BsaWI WCCGGW 6 cut(s) 137, 280, 286, 385, 448, 481
Bsc4I CCNNNNNNNGG 2 cut(s) 140, 455
Bse118I RCCGGY 2 cut(s) 148, 481
Bse1I ACTGG 1 cut(s) 172
Bse3DI GCAATG 1 cut(s) 71
BseBI CCWGG 2 cut(s) 105, 360
BseGI GGATG 4 cut(s) 469, 556, 609, 634
BseLI CCNNNNNNNGG 2 cut(s) 140, 455
BseMI GCAATG 1 cut(s) 71
BseNI ACTGG 1 cut(s) 172
BseRI GAGGAG 1 cut(s) 81
BseXI GCAGC 5 cut(s) 283, 304, 388, 406, 443
BshNI GGYRCC 1 cut(s) 283
BshTI ACCGGT 1 cut(s) 481
BsiHKCI CYCGRG 1 cut(s) 269
BslFI GGGAC 1 cut(s) 286
BslI CCNNNNNNNGG 2 cut(s) 140, 455
BsmAI GTCTC 3 cut(s) 57, 67, 124
BsmBI CGTCTC 1 cut(s) 57
BsmFI GGGAC 1 cut(s) 286
Bso31I GGTCTC 1 cut(s) 124
BsoBI CYCGRG 1 cut(s) 269
Bsp143I GATC 4 cut(s) 82, 107, 143, 451
BspACI CCGC 5 cut(s) 300, 303, 404, 416, 434
BspHI TCATGA 1 cut(s) 667
BspLI GGNNCC 4 cut(s) 285, 337, 384, 480
BspPI GGATC 3 cut(s) 115, 151, 459
BspT107I GGYRCC 1 cut(s) 283
BspTNI GGTCTC 1 cut(s) 124
BsrBI CCGCTC 1 cut(s) 416
BsrDI GCAATG 1 cut(s) 71
BsrFI RCCGGY 2 cut(s) 148, 481
BsrI ACTGG 1 cut(s) 172
BssAI RCCGGY 2 cut(s) 148, 481
BssMI GATC 4 cut(s) 82, 107, 143, 451
BssNI GRCGYC 2 cut(s) 50, 342
BssSI CACGAG 2 cut(s) 54, 529
Bst2BI CACGAG 2 cut(s) 54, 529
Bst2UI CCWGG 2 cut(s) 105, 360
Bst4CI ACNGT 1 cut(s) 31
BstACI GRCGYC 2 cut(s) 50, 342
BstBAI YACGTR 3 cut(s) 212, 248, 379
BstC8I GCNNGC 1 cut(s) 182
BstF5I GGATG 4 cut(s) 469, 556, 609, 634
BstKTI GATC 4 cut(s) 85, 110, 146, 454
BstMAI GTCTC 3 cut(s) 57, 67, 124
BstMBI GATC 4 cut(s) 82, 107, 143, 451
BstMWI GCNNNNNNNGC 3 cut(s) 302, 323, 428
BstNI CCWGG 2 cut(s) 105, 360
BstSCI CCNGG 3 cut(s) 103, 358, 490
BstSNI TACGTA 1 cut(s) 212
BstV1I GCAGC 5 cut(s) 283, 304, 388, 406, 443
BtgZI GCGATG 1 cut(s) 575
BtrI CACGTC 1 cut(s) 313
BtsCI GGATG 4 cut(s) 469, 556, 609, 634
Cac8I GCNNGC 1 cut(s) 182
CciI TCATGA 1 cut(s) 667
Cfr10I RCCGGY 2 cut(s) 148, 481
Cfr13I GGNCC 1 cut(s) 335
CseI GACGC 1 cut(s) 350
Csp6I GTAC 1 cut(s) 284
CspAI ACCGGT 1 cut(s) 481
CviAII CATG 1 cut(s) 668
CviJI RGCY 9 cut(s) 244, 268, 296, 317, 326, 368, 383, 419, 596
CviKI_1 RGCY 9 cut(s) 244, 268, 296, 317, 326, 368, 383, 419, 596
CviQI GTAC 1 cut(s) 284
DpnI GATC 4 cut(s) 84, 109, 145, 453
DpnII GATC 4 cut(s) 82, 107, 143, 451
DraI TTTAAA 1 cut(s) 330
Eco105I TACGTA 1 cut(s) 212
Eco31I GGTCTC 1 cut(s) 124
Eco47I GGWCC 1 cut(s) 335
Eco57I CTGAAG 1 cut(s) 618
Eco88I CYCGRG 1 cut(s) 269
EcoO109I RGGNCCY 1 cut(s) 335
EcoRII CCWGG 2 cut(s) 103, 358
Esp3I CGTCTC 1 cut(s) 57
FaeI CATG 1 cut(s) 671
FaiI YATR 5 cut(s) 410, 548, 572, 669, 693
FaqI GGGAC 1 cut(s) 286
FatI CATG 1 cut(s) 667
FbaI TGATCA 1 cut(s) 82
Fnu4HI GCNGC 8 cut(s) 297, 300, 303, 318, 402, 417, 420, 432
FokI GGATG 4 cut(s) 476, 563, 616, 621
Fsp4HI GCNGC 8 cut(s) 297, 300, 303, 318, 402, 417, 420, 432
FspBI CTAG 1 cut(s) 423
GluI GCNGC 8 cut(s) 297, 300, 303, 318, 402, 417, 420, 432
HgaI GACGC 1 cut(s) 350
Hin1I GRCGYC 2 cut(s) 50, 342
Hin1II CATG 1 cut(s) 671
HincII GTYRAC 1 cut(s) 585
HindII GTYRAC 1 cut(s) 585
HinfI GANTC 5 cut(s) 34, 202, 613, 653, 664
HphI GGTGA 3 cut(s) 93, 117, 138
Hpy166II GTNNAC 2 cut(s) 585, 617
Hpy188I TCNGA 3 cut(s) 24, 232, 639
Hpy188III TCNNGA 4 cut(s) 38, 56, 271, 668
Hpy8I GTNNAC 2 cut(s) 585, 617
Hpy99I CGWCG 5 cut(s) 49, 52, 314, 344, 643
HpyAV CCTTC 5 cut(s) 132, 144, 463, 593, 665
HpyCH4III ACNGT 1 cut(s) 31
HpyCH4IV ACGT 5 cut(s) 50, 211, 247, 312, 378
HpyF10VI GCNNNNNNNGC 3 cut(s) 302, 323, 428
HpySE526I ACGT 5 cut(s) 50, 211, 247, 312, 378
Hsp92I GRCGYC 2 cut(s) 50, 342
Hsp92II CATG 1 cut(s) 671
KpnI GGTACC 1 cut(s) 287
Ksp22I TGATCA 1 cut(s) 82
Kzo9I GATC 4 cut(s) 82, 107, 143, 451
LmnI GCTCC 1 cut(s) 388
Lsp1109I GCAGC 5 cut(s) 283, 304, 388, 406, 443
MaeI CTAG 1 cut(s) 423
MaeII ACGT 5 cut(s) 50, 211, 247, 312, 378
MaeIII GTNAC 2 cut(s) 212, 524
MalI GATC 4 cut(s) 84, 109, 145, 453
MbiI CCGCTC 1 cut(s) 416
MboI GATC 4 cut(s) 82, 107, 143, 451
MboII GAAGA 6 cut(s) 105, 108, 168, 515, 524, 659
MluCI AATT 4 cut(s) 16, 261, 352, 506
MlyI GAGTC 1 cut(s) 622
MmeI TCCRAC 3 cut(s) 47, 519, 662
MnlI CCTC 4 cut(s) 102, 109, 493, 547
MroXI GAANNNNTTC 1 cut(s) 483
MseI TTAA 1 cut(s) 329
MspA1I CMGCKG 1 cut(s) 434
MspR9I CCNGG 3 cut(s) 105, 360, 492
MvaI CCWGG 2 cut(s) 105, 360
MwoI GCNNNNNNNGC 3 cut(s) 302, 323, 428
NciI CCSGG 1 cut(s) 492
NdeII GATC 4 cut(s) 82, 107, 143, 451
NlaIII CATG 1 cut(s) 671
NlaIV GGNNCC 4 cut(s) 285, 337, 384, 480
PagI TCATGA 1 cut(s) 667
PcsI WCGNNNNNNNCGW 1 cut(s) 527
PdmI GAANNNNTTC 1 cut(s) 483
PfeI GAWTC 4 cut(s) 34, 202, 653, 664
PflFI GACNNNGTC 1 cut(s) 49
PinAI ACCGGT 1 cut(s) 481
PkrI GCNGC 8 cut(s) 298, 301, 304, 319, 403, 418, 421, 433
PleI GAGTC 1 cut(s) 621
PpsI GAGTC 1 cut(s) 621
Ppu21I YACGTR 3 cut(s) 212, 248, 379
PpuMI RGGWCCY 1 cut(s) 335
PsiI TTATAA 1 cut(s) 693
Psp5II RGGWCCY 1 cut(s) 335
Psp6I CCWGG 2 cut(s) 103, 358
PspGI CCWGG 2 cut(s) 103, 358
PspN4I GGNNCC 4 cut(s) 285, 337, 384, 480
PspPI GGNCC 1 cut(s) 335
PspPPI RGGWCCY 1 cut(s) 335
PsrI GAACNNNNNNTAC 2 cut(s) 517, 549
PsyI GACNNNGTC 1 cut(s) 49
RsaI GTAC 1 cut(s) 285
RsaNI GTAC 1 cut(s) 284
SaqAI TTAA 1 cut(s) 329
SatI GCNGC 8 cut(s) 297, 300, 303, 318, 402, 417, 420, 432
Sau3AI GATC 4 cut(s) 82, 107, 143, 451
Sau96I GGNCC 1 cut(s) 335
SchI GAGTC 1 cut(s) 622
ScrFI CCNGG 3 cut(s) 105, 360, 492
SgrAI CRCCGGYG 1 cut(s) 148
SinI GGWCC 1 cut(s) 335
SnaBI TACGTA 1 cut(s) 212
Sse9I AATT 4 cut(s) 16, 261, 352, 506
SsiI CCGC 5 cut(s) 300, 303, 404, 416, 434
SspMI CTAG 1 cut(s) 423
StyD4I CCNGG 3 cut(s) 103, 358, 490
TaaI ACNGT 1 cut(s) 31
TaiI ACGT 5 cut(s) 53, 214, 250, 315, 381
TaqI TCGA 2 cut(s) 5, 205
TasI AATT 4 cut(s) 16, 261, 352, 506
TauI GCSGC 3 cut(s) 302, 305, 419
TfiI GAWTC 4 cut(s) 34, 202, 653, 664
Tru1I TTAA 1 cut(s) 329
Tru9I TTAA 1 cut(s) 329
TseI GCWGC 5 cut(s) 296, 317, 401, 419, 431
TspDTI ATGAA 2 cut(s) 455, 684
TspGWI ACGGA 2 cut(s) 231, 602
Tth111I GACNNNGTC 1 cut(s) 49
VpaK11BI GGWCC 1 cut(s) 335
XapI RAATTY 2 cut(s) 261, 352
XmnI GAANNNNTTC 1 cut(s) 483
XspI CTAG 1 cut(s) 423
ZraI GACGTC 1 cut(s) 51
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.