AT5G53760

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008350
Physical Location & Seq
Forward (+)
21822323 .. 21826488
4166 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G53760.1

Sequence Viewer

Length: 1722 bp
ATGGGAGAAGGAGAAGAAAATGGAAATGAAGCAGATTCAAATGAGAGATCTTTGGCATTGTCACCTACTTGGTCTGTTGCTATTGTCTTGACTGTTTTCGTTGTTGTTTCTTTGATCGTTGAGCGCTCCATTTATCGTCTCAGCACTTGGTTGAGAAAAACAAAGAGGAAACCTATGTTTGCTGCATTAGAGAAGATGAAAGAAGAGTTGATGCTGCTTGGCTTCATTTCACTTCTATTAACAGCTACCTCAAGTACAATAGCCAATATATGTGTCCCTTCAAGTTTCTACAATGACAGATTTCTTCCGTGTACACGATCTGAGATCCAAGAGGAGCTCGAAAGCGGTTCAACTGTCAAGCGGAATCTTTTAACAAAATCCCTCTTTTTCAACATCTTTAGGAGAAGGTTGGATGTCATAAAACGAACCACCTGCAGCGAGGGGCATGAGCCGTTTGTTTCATATGAAGGCCTTGAACAGTTGCATCGCTTCATCTTTATAATGGCAGTTACTCATGTAACTTATAGCTGCTTGACCATGCTCCTTGCAATCGTCAAGATTCATAGTTGGAGGATATGGGAAGATGTAGCTCGTCTAGATAGACACGATTGCTTAACTGCGGTGGCACGTGAAAAAATATTCCGAAGGCAAACAACGTTTGTCCAGTATCATACATCAGCACCTCTGGCCAAGAATAGAATTCTTATATGGGTGACATGTTTCTTCCGGCAATTCGGACGTTCTGTTGATCGTTCTGACTATCTTACTCTCCGCAAAGGATTCATTGTGAATCACCACCTCACATTAAAGTACGATTTTCACAGCTACATGATTCGTTCTATGGAAGAAGAGTTCCAAAGGATCGTTGGTGTGAGTGGGCCGCTTTGGGGTTTCGTAGTTGCTTTCATGCTCTTTAACATAAAAGGATCAAATCTTTACTTCTGGATAGCGATTATTCCTGTAACCCTTGTTCTTCTGGTTGGTGCTAAGTTGCAACACGTAATAGCAACTTTGGCATTGGAGAATGCCGGGTTAACCGAGTATCCTTCCGGAGTAAAGCTGAGACCTCGAGATGAACTTTTCTGGTTCAATAAACCAGAACTACTCTTGTCCCTTATCCATTTCATTCTGTTTCAGAATTCTTTTGAACTGGCTTCGTTCTTCTGGTTCTGGTGGCAGTTTGGGTACAGCTCTTGCTTCCTTAAGAACCATTACCTTGTTTACTTCAGACTTCTTTTAGGGTTTGCTGGACAGTTTCTATGCAGCTACAGTACACTGCCACTATATGCACTAGTCACTCAGATGGGAACGAACTATAAAGCGGCTCTTATACCTCAGAGAATAAGAGAGACGATTCGCGGTTGGGGTAAAGCAACGAGAAGGAAAAGAAGGCACGGGTTATATGGCGATGATTCAACAGTTAGAACAGAAACAAGCACAATTGCATCACTTGAAGAATATGATCATCAAGTGCTTGACGTTACTGAAACTTCTTTCGAACAACAACGGAAACAACAAGAACAAGGTACTACTGAGCTTGAGTTGCAACCAATCCAACCTCGTAATGATTGTGTACCCAATGATACTTCAAGTAGAGTCGGAACACCTCTTCTTCGACCTTGGCTCTCCATTTCTTCACCAACAACGACCATAGAGTTGAGATCAGAACCTATGGAAACACTCTCGAGATCGTCTTCCTTGCCAAGTGAGAAGAGAGTCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0005575 GO:0005623 GO:0005886 GO:0006950 GO:0006952 GO:0008150 GO:0016020 GO:0044464 GO:0050896 GO:0071944
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

573

Amino Acids

66.35

Weight (kDa)

9.12

Isoelectric Point (pI)

51.57

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 15 - 474 1.1e-164 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 500
AarI CACCTGC 1 cut(s) 440
Acc36I ACCTGC 1 cut(s) 440
AccII CGCG 1 cut(s) 1359
AccIII TCCGGA 1 cut(s) 1049
AciI CCGC 7 cut(s) 345, 361, 620, 772, 881, 1322, 1359
AclI AACGTT 1 cut(s) 656
AclWI GGATC 3 cut(s) 319, 869, 934
AcoI YGGCCR 1 cut(s) 687
AcsI RAATTY 2 cut(s) 699, 1138
AcuI CTGAAG 1 cut(s) 1210
AcvI CACGTG 1 cut(s) 629
AfaI GTAC 7 cut(s) 256, 313, 812, 1187, 1273, 1528, 1575
AfeI AGCGCT 1 cut(s) 125
AfiI CCNNNNNNNGG 1 cut(s) 887
AflII CTTAAG 1 cut(s) 1202
AflIII ACRYGT 2 cut(s) 716, 997
AhlI ACTAGT 1 cut(s) 1291
AloI GAACNNNNNNTCC 2 cut(s) 836, 868
AluBI AGCT 9 cut(s) 245, 337, 528, 590, 825, 1060, 1191, 1266, 1537
AluI AGCT 9 cut(s) 245, 337, 528, 590, 825, 1060, 1191, 1266, 1537
Alw21I GWGCWC 1 cut(s) 339
Alw26I GTCTC 3 cut(s) 143, 1057, 1343
AlwI GGATC 3 cut(s) 319, 869, 934
Ama87I CYCGRG 2 cut(s) 1068, 1684
Aor13HI TCCGGA 1 cut(s) 1049
Aor51HI AGCGCT 1 cut(s) 125
AoxI GGCC 3 cut(s) 469, 687, 878
ApeKI GCWGC 5 cut(s) 182, 214, 435, 528, 1263
ApoI RAATTY 2 cut(s) 699, 1138
AspLEI GCGC 1 cut(s) 126
AspS9I GGNCC 1 cut(s) 878
AsuC2I CCSGG 1 cut(s) 1030
AsuHPI GGTGA 4 cut(s) 54, 724, 785, 1629
AsuII TTCGAA 1 cut(s) 1497
AvaI CYCGRG 2 cut(s) 1068, 1684
BalI TGGCCA 1 cut(s) 689
BanII GRGCYC 1 cut(s) 339
BbrPI CACGTG 1 cut(s) 629
BbsI GAAGAC 1 cut(s) 1686
Bbv12I GWGCWC 1 cut(s) 339
BbvI GCAGC 5 cut(s) 169, 201, 447, 515, 1275
BccI CCATC 1 cut(s) 1297
BceAI ACGGC 1 cut(s) 436
BcgI CGANNNNNNTGC 2 cut(s) 414, 448
BciVI GTATCC 1 cut(s) 1053
BclI TGATCA 1 cut(s) 1462
BcnI CCSGG 1 cut(s) 1030
BcoDI GTCTC 3 cut(s) 143, 1057, 1343
BcuI ACTAGT 1 cut(s) 1291
BfaI CTAG 2 cut(s) 596, 1292
BfmI CTRYAG 2 cut(s) 433, 1267
BfoI RGCGCY 1 cut(s) 127
BfrI CTTAAG 1 cut(s) 1202
BfuAI ACCTGC 1 cut(s) 440
BfuI GTATCC 1 cut(s) 1053
BglII AGATCT 1 cut(s) 47
BisI GCNGC 7 cut(s) 183, 215, 436, 529, 881, 1264, 1323
BlsI GCNGC 7 cut(s) 184, 216, 437, 530, 882, 1265, 1324
Bme1390I CCNGG 1 cut(s) 1030
BmeT110I CYCGRG 2 cut(s) 1068, 1684
BmgT120I GGNCC 1 cut(s) 878
BmrFI CCNGG 1 cut(s) 1030
BmsI GCATC 3 cut(s) 201, 493, 1454
BpiI GAAGAC 1 cut(s) 1686
Bpu14I TTCGAA 1 cut(s) 1497
BpuEI CTTGAG 2 cut(s) 235, 1559
BpuMI CCSGG 1 cut(s) 1030
BsaAI YACGTR 2 cut(s) 629, 1000
BsaI GGTCTC 1 cut(s) 1057
BsaJI CCNNGG 1 cut(s) 1619
BsaWI WCCGGW 1 cut(s) 1049
Bsc4I CCNNNNNNNGG 1 cut(s) 887
Bse1I ACTGG 2 cut(s) 664, 1155
BseAI TCCGGA 1 cut(s) 1049
BseDI CCNNGG 1 cut(s) 1619
BseGI GGATG 1 cut(s) 418
BseLI CCNNNNNNNGG 1 cut(s) 887
BseMII CTCAG 6 cut(s) 154, 312, 1052, 1313, 1349, 1524
BseNI ACTGG 2 cut(s) 664, 1155
BseRI GAGGAG 1 cut(s) 347
BseXI GCAGC 5 cut(s) 169, 201, 447, 515, 1275
Bsh1236I CGCG 1 cut(s) 1359
BshFI GGCC 3 cut(s) 471, 689, 880
BsiHKAI GWGCWC 1 cut(s) 339
BsiHKCI CYCGRG 2 cut(s) 1068, 1684
BsiSI CCGG 3 cut(s) 727, 1029, 1050
BslFI GGGAC 2 cut(s) 260, 1096
BslI CCNNNNNNNGG 1 cut(s) 887
BsmAI GTCTC 3 cut(s) 143, 1057, 1343
BsmBI CGTCTC 2 cut(s) 143, 1343
BsmFI GGGAC 2 cut(s) 260, 1096
BsmI GAATGC 1 cut(s) 1030
BsnI GGCC 3 cut(s) 471, 689, 880
Bso31I GGTCTC 1 cut(s) 1057
BsoBI CYCGRG 2 cut(s) 1068, 1684
Bsp119I TTCGAA 1 cut(s) 1497
Bsp1286I GDGCHC 1 cut(s) 339
Bsp13I TCCGGA 1 cut(s) 1049
Bsp1407I TGTACA 1 cut(s) 311
BspACI CCGC 7 cut(s) 345, 361, 620, 772, 881, 1322, 1359
BspANI GGCC 3 cut(s) 471, 689, 880
BspCNI CTCAG 6 cut(s) 153, 313, 1053, 1312, 1348, 1525
BspEI TCCGGA 1 cut(s) 1049
BspFNI CGCG 1 cut(s) 1359
BspMAI CTGCAG 1 cut(s) 437
BspMI ACCTGC 1 cut(s) 440
BspPI GGATC 3 cut(s) 319, 869, 934
BspT104I TTCGAA 1 cut(s) 1497
BspTI CTTAAG 1 cut(s) 1202
BspTNI GGTCTC 1 cut(s) 1057
BsrGI TGTACA 1 cut(s) 311
BsrI ACTGG 2 cut(s) 664, 1155
BssECI CCNNGG 1 cut(s) 1619
BssT1I CCWWGG 1 cut(s) 1619
Bst4CI ACNGT 6 cut(s) 94, 355, 480, 1254, 1271, 1420
Bst6I CTCTTC 4 cut(s) 198, 843, 1614, 1706
BstAFI CTTAAG 1 cut(s) 1202
BstAUI TGTACA 1 cut(s) 311
BstBAI YACGTR 2 cut(s) 629, 1000
BstBI TTCGAA 1 cut(s) 1497
BstDEI CTNAG 7 cut(s) 140, 321, 987, 1061, 1299, 1335, 1533
BstF5I GGATG 1 cut(s) 418
BstFNI CGCG 1 cut(s) 1359
BstH2I RGCGCY 1 cut(s) 127
BstHHI GCGC 1 cut(s) 126
BstMAI GTCTC 3 cut(s) 143, 1057, 1343
BstMWI GCNNNNNNNGC 3 cut(s) 686, 1013, 1543
BstNSI RCATGY 1 cut(s) 720
BstSCI CCNGG 1 cut(s) 1028
BstSFI CTRYAG 2 cut(s) 433, 1267
BstUI CGCG 1 cut(s) 1359
BstV1I GCAGC 5 cut(s) 169, 201, 447, 515, 1275
BstV2I GAAGAC 1 cut(s) 1686
BstX2I RGATCY 2 cut(s) 47, 324
BstYI RGATCY 2 cut(s) 47, 324
BsuI GTATCC 1 cut(s) 1053
BsuRI GGCC 3 cut(s) 471, 689, 880
BtgZI GCGATG 2 cut(s) 470, 1422
BtsCI GGATG 1 cut(s) 418
BtsI GCAGTG 1 cut(s) 1274
BtsIMutI CAGTG 1 cut(s) 1274
BveI ACCTGC 1 cut(s) 440
CfoI GCGC 1 cut(s) 126
Cfr13I GGNCC 1 cut(s) 878
Csp6I GTAC 7 cut(s) 255, 312, 811, 1186, 1272, 1527, 1574
CviAII CATG 6 cut(s) 446, 515, 538, 717, 829, 907
CviQI GTAC 7 cut(s) 255, 312, 811, 1186, 1272, 1527, 1574
DdeI CTNAG 7 cut(s) 140, 321, 987, 1061, 1299, 1335, 1533
EaeI YGGCCR 1 cut(s) 687
Eam1104I CTCTTC 4 cut(s) 198, 843, 1614, 1706
EarI CTCTTC 4 cut(s) 198, 843, 1614, 1706
Ecl136II GAGCTC 1 cut(s) 337
Eco130I CCWWGG 1 cut(s) 1619
Eco147I AGGCCT 1 cut(s) 471
Eco24I GRGCYC 1 cut(s) 339
Eco31I GGTCTC 1 cut(s) 1057
Eco47III AGCGCT 1 cut(s) 125
Eco53kI GAGCTC 1 cut(s) 337
Eco57I CTGAAG 1 cut(s) 1210
Eco72I CACGTG 1 cut(s) 629
Eco88I CYCGRG 2 cut(s) 1068, 1684
EcoICRI GAGCTC 1 cut(s) 337
EcoRI GAATTC 2 cut(s) 699, 1138
EcoT14I CCWWGG 1 cut(s) 1619
EcoT38I GRGCYC 1 cut(s) 339
ErhI CCWWGG 1 cut(s) 1619
Esp3I CGTCTC 2 cut(s) 143, 1343
FaeI CATG 6 cut(s) 449, 518, 541, 720, 832, 910
FalI AAGNNNNNCTT 2 cut(s) 1311, 1343
FaqI GGGAC 2 cut(s) 260, 1096
FatI CATG 6 cut(s) 445, 514, 537, 716, 828, 906
FauNDI CATATG 1 cut(s) 463
FbaI TGATCA 1 cut(s) 1462
Fnu4HI GCNGC 7 cut(s) 183, 215, 436, 529, 881, 1264, 1323
FokI GGATG 1 cut(s) 425
FriOI GRGCYC 1 cut(s) 339
Fsp4HI GCNGC 7 cut(s) 183, 215, 436, 529, 881, 1264, 1323
FspBI CTAG 2 cut(s) 596, 1292
GlaI GCGC 1 cut(s) 125
GluI GCNGC 7 cut(s) 183, 215, 436, 529, 881, 1264, 1323
HaeII RGCGCY 1 cut(s) 127
HaeIII GGCC 3 cut(s) 471, 689, 880
HapII CCGG 3 cut(s) 727, 1029, 1050
HhaI GCGC 1 cut(s) 126
Hin1II CATG 6 cut(s) 449, 518, 541, 720, 832, 910
Hin6I GCGC 1 cut(s) 124
HinP1I GCGC 1 cut(s) 124
HincII GTYRAC 1 cut(s) 1035
HindII GTYRAC 1 cut(s) 1035
HpaI GTTAAC 1 cut(s) 1035
HpaII CCGG 3 cut(s) 727, 1029, 1050
HphI GGTGA 4 cut(s) 54, 724, 785, 1629
Hpy166II GTNNAC 6 cut(s) 312, 314, 1035, 1222, 1274, 1574
Hpy188III TCNNGA 8 cut(s) 88, 556, 596, 943, 1050, 1070, 1684, 1686
Hpy8I GTNNAC 6 cut(s) 312, 314, 1035, 1222, 1274, 1574
HpyAV CCTTC 7 cut(s) 288, 399, 461, 639, 1056, 1374, 1383
HpyCH4III ACNGT 6 cut(s) 94, 355, 480, 1254, 1271, 1420
HpyCH4IV ACGT 5 cut(s) 628, 656, 739, 999, 1479
HpyCH4V TGCA 9 cut(s) 185, 435, 484, 548, 994, 1263, 1289, 1445, 1546
HpyF10VI GCNNNNNNNGC 3 cut(s) 686, 1013, 1543
HpyF3I CTNAG 7 cut(s) 140, 321, 987, 1061, 1299, 1335, 1533
HpySE526I ACGT 5 cut(s) 628, 656, 739, 999, 1479
Hsp92II CATG 6 cut(s) 449, 518, 541, 720, 832, 910
HspAI GCGC 1 cut(s) 124
Kpn2I TCCGGA 1 cut(s) 1049
Ksp22I TGATCA 1 cut(s) 1462
KspAI GTTAAC 1 cut(s) 1035
LmnI GCTCC 3 cut(s) 131, 334, 546
Lsp1109I GCAGC 5 cut(s) 169, 201, 447, 515, 1275
LweI GCATC 3 cut(s) 201, 493, 1454
MaeI CTAG 2 cut(s) 596, 1292
MaeII ACGT 5 cut(s) 628, 656, 739, 999, 1479
MaeIII GTNAC 7 cut(s) 60, 508, 517, 712, 961, 1294, 1480
MfeI CAATTG 1 cut(s) 1440
MflI RGATCY 2 cut(s) 47, 324
MhlI GDGCHC 1 cut(s) 339
MlsI TGGCCA 1 cut(s) 689
MluCI AATT 4 cut(s) 699, 731, 1138, 1440
MluNI TGGCCA 1 cut(s) 689
MlyI GAGTC 1 cut(s) 1605
MmeI TCCRAC 4 cut(s) 390, 548, 1579, 1579
Mox20I TGGCCA 1 cut(s) 689
MroI TCCGGA 1 cut(s) 1049
MscI TGGCCA 1 cut(s) 689
MseI TTAA 7 cut(s) 239, 371, 614, 806, 915, 1034, 1203
MslI CAYNNNNRTG 1 cut(s) 1301
Msp20I TGGCCA 1 cut(s) 689
MspCI CTTAAG 1 cut(s) 1202
MspI CCGG 3 cut(s) 727, 1029, 1050
MspR9I CCNGG 1 cut(s) 1030
MunI CAATTG 1 cut(s) 1440
Mva1269I GAATGC 1 cut(s) 1030
MvnI CGCG 1 cut(s) 1359
MwoI GCNNNNNNNGC 3 cut(s) 686, 1013, 1543
NciI CCSGG 1 cut(s) 1030
NdeI CATATG 1 cut(s) 463
NlaIII CATG 6 cut(s) 449, 518, 541, 720, 832, 910
NmuCI GTSAC 3 cut(s) 60, 712, 1294
NspI RCATGY 1 cut(s) 720
NspV TTCGAA 1 cut(s) 1497
PaeR7I CTCGAG 2 cut(s) 1068, 1684
PaqCI CACCTGC 1 cut(s) 440
PceI AGGCCT 1 cut(s) 471
PciI ACATGT 1 cut(s) 716
PctI GAATGC 1 cut(s) 1030
PfeI GAWTC 8 cut(s) 35, 364, 559, 780, 790, 832, 1354, 1412
PkrI GCNGC 7 cut(s) 184, 216, 437, 530, 882, 1265, 1324
PleI GAGTC 1 cut(s) 1604
PmaCI CACGTG 1 cut(s) 629
PmlI CACGTG 1 cut(s) 629
PpsI GAGTC 1 cut(s) 1604
Ppu21I YACGTR 2 cut(s) 629, 1000
PscI ACATGT 1 cut(s) 716
PsiI TTATAA 1 cut(s) 500
Psp124BI GAGCTC 1 cut(s) 339
Psp1406I AACGTT 1 cut(s) 656
PspCI CACGTG 1 cut(s) 629
PspPI GGNCC 1 cut(s) 878
PsrI GAACNNNNNNTAC 2 cut(s) 954, 986
PstI CTGCAG 1 cut(s) 437
PsuI RGATCY 2 cut(s) 47, 324
RsaI GTAC 7 cut(s) 256, 313, 812, 1187, 1273, 1528, 1575
RsaNI GTAC 7 cut(s) 255, 312, 811, 1186, 1272, 1527, 1574
RseI CAYNNNNRTG 1 cut(s) 1301
SacI GAGCTC 1 cut(s) 339
SaqAI TTAA 7 cut(s) 239, 371, 614, 806, 915, 1034, 1203
SatI GCNGC 7 cut(s) 183, 215, 436, 529, 881, 1264, 1323
Sau96I GGNCC 1 cut(s) 878
SchI GAGTC 1 cut(s) 1605
ScrFI CCNGG 1 cut(s) 1030
SduI GDGCHC 1 cut(s) 339
SfaNI GCATC 3 cut(s) 201, 493, 1454
SfcI CTRYAG 2 cut(s) 433, 1267
Sfr274I CTCGAG 2 cut(s) 1068, 1684
SfuI TTCGAA 1 cut(s) 1497
SlaI CTCGAG 2 cut(s) 1068, 1684
SmiMI CAYNNNNRTG 1 cut(s) 1301
SmlI CTYRAG 5 cut(s) 250, 1068, 1202, 1538, 1684
SmoI CTYRAG 5 cut(s) 250, 1068, 1202, 1538, 1684
SpeI ACTAGT 1 cut(s) 1291
Sse9I AATT 4 cut(s) 699, 731, 1138, 1440
SseBI AGGCCT 1 cut(s) 471
SsiI CCGC 7 cut(s) 345, 361, 620, 772, 881, 1322, 1359
SspI AATATT 1 cut(s) 639
SspMI CTAG 2 cut(s) 596, 1292
SstI GAGCTC 1 cut(s) 339
StuI AGGCCT 1 cut(s) 471
StyD4I CCNGG 1 cut(s) 1028
StyI CCWWGG 1 cut(s) 1619
TaaI ACNGT 6 cut(s) 94, 355, 480, 1254, 1271, 1420
TaiI ACGT 5 cut(s) 631, 659, 742, 1002, 1482
TaqI TCGA 5 cut(s) 339, 1069, 1497, 1615, 1685
TasI AATT 4 cut(s) 699, 731, 1138, 1440
TatI WGTACW 3 cut(s) 254, 311, 1271
TauI GCSGC 2 cut(s) 883, 1325
TfiI GAWTC 8 cut(s) 35, 364, 559, 780, 790, 832, 1354, 1412
Tru1I TTAA 7 cut(s) 239, 371, 614, 806, 915, 1034, 1203
Tru9I TTAA 7 cut(s) 239, 371, 614, 806, 915, 1034, 1203
TscAI CASTG 1 cut(s) 1281
TseFI GTSAC 3 cut(s) 60, 712, 1294
TseI GCWGC 5 cut(s) 182, 214, 435, 528, 1263
Tsp45I GTSAC 3 cut(s) 60, 712, 1294
TspGWI ACGGA 2 cut(s) 297, 1522
TspRI CASTG 1 cut(s) 1281
Vha464I CTTAAG 1 cut(s) 1202
XapI RAATTY 2 cut(s) 699, 1138
XbaI TCTAGA 1 cut(s) 595
XceI RCATGY 1 cut(s) 720
XcmI CCANNNNNNNNNTGG 1 cut(s) 863
XhoI CTCGAG 2 cut(s) 1068, 1684
XspI CTAG 2 cut(s) 596, 1292
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.