MD00G1089600.v1.1

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr00
OG0008350
Physical Location & Seq
Reverse (-)
18492418 .. 18499429
7012 bp
Loading structure...
UTR
Exon/CDS
Intron
MD00G1089600.v1.1.491

Sequence Viewer

Length: 1608 bp
ATGGCGAAAGATGAACAAGAAATGAGATCGTTGGCCTTGATTCCAACATGGTCTGTTGCTACTGTGTTGACAATTTTGTTGGCTGCTTCTCTGCTTGTGGAGCGCGGAATCCATAGGCTAAGCTATTGGCTGCGGAAAACTAATCGTAAACCCTTGCTTGAAGCTGTGGAGAAAATGAAGGAAGAGTTGATGTTACTTGGGTTTATCTCTCTCCTTTTGACGGCTACCTCAGGCATGATATCCAGTATTTGCATTCCATCAAAGTTCTACGATAGCAATTTTTCACCATGCTCTAGGTCTGAGATTAATGAAGAAACTGAAAGTAATTCGTCCCAAGAAAACCGTATTCCCTGCAAAGAGGGTTATGAACCAATTGTTTCATATGAAGGCCTTGAGCAGTTGCACCGCTTCATCTTTATCATGGCAATAACACATATATCCTACAGCTGCTTAACGATGTTGCTGGCAATAGTGAAGATTCACAGCTGGAGAGTGTGGGAGGATGAGGCTCGAAAGGACCGACATGATTCATTGACTGAGATCACACGGCAGTTGACAATGCGAAGGCAGTCTAGCTTTGTAAATCCGTCAACTCCTGTTGTCAGGAACGACATTTTTATCTGGGTGATATGTTTCTTCCGGCAATTCGGGCATTCAGTGTTTCGTGCTGACTATTTAACACTCCGCAAGGGTTTCATCATGAATCACAACCTTTCGCCAAAGTATGACTTTCACAGCTATATGATTCGGTCTATGGAAGAGGAATTCCAAAGGATTGTTGGTGTGAGTGCCACACTCTGGGGATTTGTTGTTGCTTTCATGCTGCTTAATGTAAAAGGGTCTAATCTTTATTTCTGGATTGCAATCATTCCAATTGCGCTAGTTCTTCTTGTGGGAATGAAGCTGCAGCATATTATTGCAACCCTAGCATTGGAGAATGCTGGTATAACTGGATCTCGTCGAGAAGGAAAGCTGAGGCCTCGGGATGATCTTTTCTGGTTCAAGAAGCCTGAACTTTTGCTGTCCTTGATCCATTTTTGTTTATTCCAGAATGCATTTGAATTGGCTACATTCTTTTGGTTTTGGTGGCAGTTTGGGTATAATTCTTGCTTTATCCGAAAGCATTGGCTCGTGTATATGAGGCTGATTTTAGGGTTCGCCGGGCAGTTCTTGTGCAGCTACATCACCTTGCCACTGTATGCCTTGGTTACTCAGATGGGAACGAATTATAAGGCGGCAATAATTCCACAAAGGATAAGAGAAACAATTCATGGATGGGGAGAGTCAGCTAGGAGAAGAAGAAGGCTAGGCATGTACAACGATGGTTCAACCATTCGCACAGAAGCAAGCACAGAAGTATTGCTCGAGGAAGATGATCATCAAACGCTCGATTGCCCTGCAGATGATGCTAATACGCATGATGGAATTGAAGTGCAATCGGTTTCTAGTTCTCCGGCCAGTCCTTGCCCGGTTGATAATGAAACCTCAAGTACGCCAGCTACACCCCTTCTTCAACCATCGGCTTCTATTTCTTCTTCATCTGTTACATTGACTATTCAAAGAGAAGGTATTCAAAGATCCGCATCAATGCCAGCTAGAAGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

536

Amino Acids

61.39

Weight (kDa)

8.0

Isoelectric Point (pI)

53.05

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 9 - 441 1e-152 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1230
AccB7I CCANNNNNTGG 1 cut(s) 798
AccII CGCG 1 cut(s) 105
AciI CCGC 6 cut(s) 105, 133, 406, 685, 1235, 1581
AclWI GGATC 3 cut(s) 961, 1024, 1572
AcoI YGGCCR 1 cut(s) 1455
AcsI RAATTY 1 cut(s) 764
AfaI GTAC 2 cut(s) 1316, 1492
AfiI CCNNNNNNNGG 3 cut(s) 220, 798, 931
AgsI TTSAA 8 cut(s) 161, 1003, 1061, 1329, 1430, 1514, 1559, 1574
AlwI GGATC 3 cut(s) 961, 1024, 1572
Ama87I CYCGRG 2 cut(s) 981, 1364
AoxI GGCC 4 cut(s) 33, 388, 977, 1455
ApeKI GCWGC 7 cut(s) 83, 130, 447, 823, 904, 907, 1176
ApoI RAATTY 1 cut(s) 764
AseI ATTAAT 1 cut(s) 306
Asp700I GAANNNNTTC 2 cut(s) 1266, 1569
AspLEI GCGC 2 cut(s) 105, 880
AspS9I GGNCC 1 cut(s) 517
AsuC2I CCSGG 2 cut(s) 1162, 1469
AsuHPI GGTGA 3 cut(s) 276, 637, 1177
AvaI CYCGRG 2 cut(s) 981, 1364
AvaII GGWCC 1 cut(s) 517
AxyI CCTNAGG 1 cut(s) 229
BauI CACGAG 1 cut(s) 1130
BbvCI CCTCAGC 1 cut(s) 974
BbvI GCAGC 7 cut(s) 70, 117, 434, 810, 891, 919, 1188
BccI CCATC 6 cut(s) 265, 1210, 1269, 1316, 1415, 1525
BceAI ACGGC 2 cut(s) 237, 563
BcgI CGANNNNNNTGC 2 cut(s) 1379, 1413
BclI TGATCA 1 cut(s) 1375
BcnI CCSGG 2 cut(s) 1162, 1469
BfaI CTAG 8 cut(s) 294, 573, 881, 926, 1290, 1307, 1446, 1596
BfmI CTRYAG 3 cut(s) 442, 905, 1398
BisI GCNGC 8 cut(s) 84, 131, 448, 824, 905, 908, 1177, 1236
BlpI GCTNAGC 1 cut(s) 119
BlsI GCNGC 8 cut(s) 85, 132, 449, 825, 906, 909, 1178, 1237
Bme1390I CCNGG 2 cut(s) 1162, 1469
Bme18I GGWCC 1 cut(s) 517
BmeT110I CYCGRG 2 cut(s) 981, 1364
BmgT120I GGNCC 1 cut(s) 517
BmrFI CCNGG 2 cut(s) 1162, 1469
BmsI GCATC 2 cut(s) 1396, 1592
BpmI CTGGAG 1 cut(s) 508
Bpu10I CCTNAGC 1 cut(s) 974
Bpu1102I GCTNAGC 1 cut(s) 119
BpuEI CTTGAG 2 cut(s) 413, 1471
BpuMI CCSGG 2 cut(s) 1162, 1469
BsaBI GATNNNNATC 3 cut(s) 863, 1377, 1582
BsaJI CCNNGG 2 cut(s) 980, 1203
Bsc4I CCNNNNNNNGG 3 cut(s) 220, 798, 931
Bse1I ACTGG 3 cut(s) 243, 955, 1458
Bse21I CCTNAGG 1 cut(s) 229
Bse8I GATNNNNATC 3 cut(s) 863, 1377, 1582
BseDI CCNNGG 2 cut(s) 980, 1203
BseGI GGATG 3 cut(s) 508, 991, 1280
BseJI GATNNNNATC 3 cut(s) 863, 1377, 1582
BseLI CCNNNNNNNGG 3 cut(s) 220, 798, 931
BseMII CTCAG 5 cut(s) 243, 291, 528, 965, 1226
BseNI ACTGG 3 cut(s) 243, 955, 1458
BseXI GCAGC 7 cut(s) 70, 117, 434, 810, 891, 919, 1188
BsgI GTGCAG 1 cut(s) 1195
Bsh1236I CGCG 1 cut(s) 105
BshFI GGCC 4 cut(s) 35, 390, 979, 1457
BsiHKCI CYCGRG 2 cut(s) 981, 1364
BsiSI CCGG 4 cut(s) 640, 1161, 1454, 1469
BslFI GGGAC 1 cut(s) 316
BslI CCNNNNNNNGG 3 cut(s) 220, 798, 931
BsmFI GGGAC 1 cut(s) 316
BsmI GAATGC 4 cut(s) 252, 652, 943, 1057
BsnI GGCC 4 cut(s) 35, 390, 979, 1457
BsoBI CYCGRG 2 cut(s) 981, 1364
Bsp1407I TGTACA 1 cut(s) 1314
Bsp143I GATC 7 cut(s) 26, 540, 953, 988, 1029, 1375, 1577
Bsp1720I GCTNAGC 1 cut(s) 119
BspACI CCGC 6 cut(s) 105, 133, 406, 685, 1235, 1581
BspANI GGCC 4 cut(s) 35, 390, 979, 1457
BspCNI CTCAG 5 cut(s) 242, 292, 529, 966, 1225
BspFNI CGCG 1 cut(s) 105
BspHI TCATGA 1 cut(s) 699
BspMAI CTGCAG 2 cut(s) 909, 1402
BspPI GGATC 3 cut(s) 961, 1024, 1572
BsrGI TGTACA 1 cut(s) 1314
BsrI ACTGG 3 cut(s) 243, 955, 1458
BssECI CCNNGG 2 cut(s) 980, 1203
BssMI GATC 7 cut(s) 26, 540, 953, 988, 1029, 1375, 1577
BssSI CACGAG 1 cut(s) 1130
BssT1I CCWWGG 1 cut(s) 1203
Bst2BI CACGAG 1 cut(s) 1130
Bst4CI ACNGT 3 cut(s) 64, 344, 1197
Bst6I CTCTTC 2 cut(s) 177, 753
BstAPI GCANNNNNTGC 1 cut(s) 1406
BstAUI TGTACA 1 cut(s) 1314
BstC8I GCNNGC 4 cut(s) 465, 1348, 1497, 1593
BstDEI CTNAG 6 cut(s) 119, 229, 300, 537, 974, 1212
BstF5I GGATG 3 cut(s) 508, 991, 1280
BstFNI CGCG 1 cut(s) 105
BstHHI GCGC 2 cut(s) 105, 880
BstKTI GATC 7 cut(s) 29, 543, 956, 991, 1032, 1378, 1580
BstMBI GATC 7 cut(s) 26, 540, 953, 988, 1029, 1375, 1577
BstMWI GCNNNNNNNGC 4 cut(s) 100, 649, 926, 1406
BstNSI RCATGY 1 cut(s) 1315
BstSCI CCNGG 2 cut(s) 1160, 1467
BstSFI CTRYAG 3 cut(s) 442, 905, 1398
BstUI CGCG 1 cut(s) 105
BstV1I GCAGC 7 cut(s) 70, 117, 434, 810, 891, 919, 1188
BstX2I RGATCY 2 cut(s) 953, 1577
BstYI RGATCY 2 cut(s) 953, 1577
Bsu36I CCTNAGG 1 cut(s) 229
BsuRI GGCC 4 cut(s) 35, 390, 979, 1457
BtsCI GGATG 3 cut(s) 508, 991, 1280
BtsIMutI CAGTG 2 cut(s) 663, 1193
Cac8I GCNNGC 4 cut(s) 465, 1348, 1497, 1593
CciI TCATGA 1 cut(s) 699
CfoI GCGC 2 cut(s) 105, 880
Cfr13I GGNCC 1 cut(s) 517
Csp6I GTAC 2 cut(s) 1315, 1491
CviQI GTAC 2 cut(s) 1315, 1491
DdeI CTNAG 6 cut(s) 119, 229, 300, 537, 974, 1212
DpnI GATC 7 cut(s) 28, 542, 955, 990, 1031, 1377, 1579
DpnII GATC 7 cut(s) 26, 540, 953, 988, 1029, 1375, 1577
EaeI YGGCCR 1 cut(s) 1455
Eam1104I CTCTTC 2 cut(s) 177, 753
EarI CTCTTC 2 cut(s) 177, 753
Eco130I CCWWGG 1 cut(s) 1203
Eco147I AGGCCT 2 cut(s) 390, 979
Eco32I GATATC 1 cut(s) 240
Eco47I GGWCC 1 cut(s) 517
Eco81I CCTNAGG 1 cut(s) 229
Eco88I CYCGRG 2 cut(s) 981, 1364
EcoRI GAATTC 1 cut(s) 764
EcoRV GATATC 1 cut(s) 240
EcoT14I CCWWGG 1 cut(s) 1203
EcoT22I ATGCAT 1 cut(s) 1057
ErhI CCWWGG 1 cut(s) 1203
FalI AAGNNNNNCTT 2 cut(s) 713, 745
FaqI GGGAC 1 cut(s) 316
FauNDI CATATG 1 cut(s) 382
FbaI TGATCA 1 cut(s) 1375
Fnu4HI GCNGC 8 cut(s) 84, 131, 448, 824, 905, 908, 1177, 1236
FokI GGATG 3 cut(s) 515, 998, 1287
Fsp4HI GCNGC 8 cut(s) 84, 131, 448, 824, 905, 908, 1177, 1236
FspBI CTAG 8 cut(s) 294, 573, 881, 926, 1290, 1307, 1446, 1596
GlaI GCGC 2 cut(s) 104, 879
GluI GCNGC 8 cut(s) 84, 131, 448, 824, 905, 908, 1177, 1236
GsuI CTGGAG 1 cut(s) 508
HaeIII GGCC 4 cut(s) 35, 390, 979, 1457
HapII CCGG 4 cut(s) 640, 1161, 1454, 1469
HhaI GCGC 2 cut(s) 105, 880
Hin6I GCGC 2 cut(s) 103, 878
HinP1I GCGC 2 cut(s) 103, 878
HincII GTYRAC 3 cut(s) 69, 555, 591
HindII GTYRAC 3 cut(s) 69, 555, 591
HinfI GANTC 7 cut(s) 40, 108, 478, 527, 703, 745, 1283
HpaII CCGG 4 cut(s) 640, 1161, 1454, 1469
HphI GGTGA 3 cut(s) 276, 637, 1177
Hpy166II GTNNAC 4 cut(s) 69, 149, 555, 591
Hpy188I TCNGA 3 cut(s) 301, 1118, 1215
Hpy188III TCNNGA 7 cut(s) 604, 700, 856, 962, 983, 1003, 1048
Hpy8I GTNNAC 4 cut(s) 69, 149, 555, 591
Hpy99I CGWCG 1 cut(s) 963
HpyAV CCTTC 8 cut(s) 172, 380, 558, 959, 1296, 1517, 1559, 1593
HpyCH4III ACNGT 3 cut(s) 64, 344, 1197
HpyF10VI GCNNNNNNNGC 4 cut(s) 100, 649, 926, 1406
HpyF3I CTNAG 6 cut(s) 119, 229, 300, 537, 974, 1212
HspAI GCGC 2 cut(s) 103, 878
Ksp22I TGATCA 1 cut(s) 1375
Kzo9I GATC 7 cut(s) 26, 540, 953, 988, 1029, 1375, 1577
LmnI GCTCC 1 cut(s) 100
Lsp1109I GCAGC 7 cut(s) 70, 117, 434, 810, 891, 919, 1188
LweI GCATC 2 cut(s) 1396, 1592
MaeI CTAG 8 cut(s) 294, 573, 881, 926, 1290, 1307, 1446, 1596
MaeIII GTNAC 3 cut(s) 192, 1207, 1543
MalI GATC 7 cut(s) 28, 542, 955, 990, 1031, 1377, 1579
MboI GATC 7 cut(s) 26, 540, 953, 988, 1029, 1375, 1577
MfeI CAATTG 2 cut(s) 372, 873
MflI RGATCY 2 cut(s) 953, 1577
MlyI GAGTC 1 cut(s) 1292
MmeI TCCRAC 1 cut(s) 68
Mph1103I ATGCAT 1 cut(s) 1057
MroXI GAANNNNTTC 2 cut(s) 1266, 1569
MseI TTAA 4 cut(s) 306, 452, 677, 828
MspA1I CMGCKG 2 cut(s) 447, 486
MspI CCGG 4 cut(s) 640, 1161, 1454, 1469
MspR9I CCNGG 2 cut(s) 1162, 1469
MunI CAATTG 2 cut(s) 372, 873
Mva1269I GAATGC 4 cut(s) 252, 652, 943, 1057
MvnI CGCG 1 cut(s) 105
MwoI GCNNNNNNNGC 4 cut(s) 100, 649, 926, 1406
NciI CCSGG 2 cut(s) 1162, 1469
NdeI CATATG 1 cut(s) 382
NdeII GATC 7 cut(s) 26, 540, 953, 988, 1029, 1375, 1577
NsiI ATGCAT 1 cut(s) 1057
NspI RCATGY 1 cut(s) 1315
PaeR7I CTCGAG 1 cut(s) 1364
PagI TCATGA 1 cut(s) 699
PceI AGGCCT 2 cut(s) 390, 979
PcsI WCGNNNNNNNCGW 1 cut(s) 517
PctI GAATGC 4 cut(s) 252, 652, 943, 1057
PdmI GAANNNNTTC 2 cut(s) 1266, 1569
PfeI GAWTC 6 cut(s) 40, 108, 478, 527, 703, 745
PflMI CCANNNNNTGG 1 cut(s) 798
PkrI GCNGC 8 cut(s) 85, 132, 449, 825, 906, 909, 1178, 1237
PleI GAGTC 1 cut(s) 1291
PpsI GAGTC 1 cut(s) 1291
PshBI ATTAAT 1 cut(s) 306
PsiI TTATAA 1 cut(s) 1230
PspPI GGNCC 1 cut(s) 517
PspXI VCTCGAGB 1 cut(s) 1364
PstI CTGCAG 2 cut(s) 909, 1402
PsuI RGATCY 2 cut(s) 953, 1577
PvuII CAGCTG 2 cut(s) 447, 486
RsaI GTAC 2 cut(s) 1316, 1492
RsaNI GTAC 2 cut(s) 1315, 1491
SaqAI TTAA 4 cut(s) 306, 452, 677, 828
SatI GCNGC 8 cut(s) 84, 131, 448, 824, 905, 908, 1177, 1236
Sau3AI GATC 7 cut(s) 26, 540, 953, 988, 1029, 1375, 1577
Sau96I GGNCC 1 cut(s) 517
SchI GAGTC 1 cut(s) 1292
ScrFI CCNGG 2 cut(s) 1162, 1469
SfaNI GCATC 2 cut(s) 1396, 1592
SfcI CTRYAG 3 cut(s) 442, 905, 1398
Sfr274I CTCGAG 1 cut(s) 1364
SinI GGWCC 1 cut(s) 517
SlaI CTCGAG 1 cut(s) 1364
SmlI CTYRAG 3 cut(s) 392, 1364, 1486
SmoI CTYRAG 3 cut(s) 392, 1364, 1486
SseBI AGGCCT 2 cut(s) 390, 979
SsiI CCGC 6 cut(s) 105, 133, 406, 685, 1235, 1581
SspMI CTAG 8 cut(s) 294, 573, 881, 926, 1290, 1307, 1446, 1596
StuI AGGCCT 2 cut(s) 390, 979
StyD4I CCNGG 2 cut(s) 1160, 1467
StyI CCWWGG 1 cut(s) 1203
TaaI ACNGT 3 cut(s) 64, 344, 1197
TaqI TCGA 4 cut(s) 511, 961, 1365, 1389
TaqII GACCGA 2 cut(s) 534, 738
TatI WGTACW 1 cut(s) 1314
TauI GCSGC 1 cut(s) 1238
TfiI GAWTC 6 cut(s) 40, 108, 478, 527, 703, 745
Tru1I TTAA 4 cut(s) 306, 452, 677, 828
Tru9I TTAA 4 cut(s) 306, 452, 677, 828
TscAI CASTG 2 cut(s) 663, 1200
TseI GCWGC 7 cut(s) 83, 130, 447, 823, 904, 907, 1176
TspGWI ACGGA 1 cut(s) 576
TspRI CASTG 2 cut(s) 663, 1200
Van91I CCANNNNNTGG 1 cut(s) 798
VpaK11BI GGWCC 1 cut(s) 517
VspI ATTAAT 1 cut(s) 306
XapI RAATTY 1 cut(s) 764
XceI RCATGY 1 cut(s) 1315
XcmI CCANNNNNNNNNTGG 1 cut(s) 776
XhoI CTCGAG 1 cut(s) 1364
XmnI GAANNNNTTC 2 cut(s) 1266, 1569
XspI CTAG 8 cut(s) 294, 573, 881, 926, 1290, 1307, 1446, 1596
Zsp2I ATGCAT 1 cut(s) 1057
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.