Rh5DG245100

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5D
OG0008350
Physical Location & Seq
Reverse (-)
28005906 .. 28013000
7095 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5DG245100.1

Sequence Viewer

Length: 1659 bp
ATGGCCGAAGATGCACAAGAAATGCGCTCTTTGGCCTTGACACCAACATGGTCTGTTGCTACTGTGTTGACAATTTTTGTGGCTGTTTCTCTGCTTGTGGAGCGCAGCATTCATCGGTTAAGCCATTGGTTGCGGAAAACTAATCGTAAGCCCTTGCTTGAAGCTGTGGAGAAAATGAAGGAAGAGTTGATGCTTCTTGGATTTATCTCTCTTCTTTTGACGGCTACCTCAAGCACGATAGCCAATATTTGCATTCCATCAAACTTCTATGATAGCAAGTTTTCTCCATGTACAAGAAAAGAGATTGATGAAGAAAATGAAACTAAGACATCTGAGGAACGCAAACTATTGATGTTTACCGTTCTGCCTCATACGTTTAGGAGAATGTTAAATGGCTTGAATGCTAATACCTGCAAAGAGGGTTATGAGCCATTCGTTTCATATCAAGGTCTTGAGCAGTTGCACCGCTTCATCTTTGTCATGGCAATAACACATGTATCCTACAGCTGCTTAACGATGTTGCTGGCAATAGTGAAGATTCACGGTTGGAGAGCATGGGAGGATGCGGCTCACATGGACAGGCATGATTCCTTGACTGAAATCACACGGCAGATGACAATGCGAAGGCAGTCTACCTTTGTAAATACATCAAATCCACTGGCCAGGAATAGTTTTTTAATCTGGGTGATATGCTTCTTCAGGCAATTCGGGCATTCAGTGGTTCGTGCTGACTACTTAACACTCCGCAAGGGTTTCATCATGAATCACAACCTTCCACCAAAGTATGATTTTCACAGCTATATGATTCGATCTATGGAAGAGGAATTCCAAAGAATAGTTGGTGTGAGCGCTCCACTCTGGGGATTTGTCGTTGCTTTCATGCTGTTTAATGTACAAGGGTCTAATCTTTATTTCTGGATTGCAATCATTCCAATTACTCTAGTCCTTCTTGTGGGAATGAAGTTGCAGCATGTTATTGCAACCCTTGCTTTGGAGAATGCTGGTATATCTGGATCCTATCCAGGAGCAAAGCTGAGGCCTCGAGATGATCTTTTCTGGTTCAAGAAGCCAGAACTTCTGTTGTCCTTGATACATTTTTGTTTGTTCCAGAATGCTTTTGAGTTGGCTTCATTCTTTTGGTTTTGGTGGCAGTTTGGGTATAGTTCTTGCTTCATTCGGAATCATACACTTGTGTATTTGAGGCTGATTTTAGGGTTTGCTGGGCAATTCCTGTGCAGCTACAGCACCTTGCCACTGTATGCCTTGGTTACTCAGATGGGAACAAATTATAAGGCCGCACTAATCCCACAAAGGATAAGAGAAACCATACATGGGTGGAAAAAAGCAGCTAGGAGGAAAAGAAGGCTAGGCACATACAATGACGATTCAACCATACACACATGTACAGACACAAGCACAGTAGTGTCGGTTGAGGACGATGATCGTGATTTTTTCGATGGCCCAGCAACTGATGCCAATACACATAATGGAGTTGAGTTGCAACCAGTTTCTAGTGTGATGAACAGTCCTTTCCCAGTTGCTAACGAAACATCAAGTAGGGCAGGTACACCCCTTCTTCGTCCATCGGCATCTGTTTCTGCATCAGTAGTATTGAATTTTCAACCAGAAACTGTTCCAAGGTCTGCATCAATGAAGTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

552

Amino Acids

63.15

Weight (kDa)

8.93

Isoelectric Point (pI)

43.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 9 - 470 3.9e-168 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1290
Acc36I ACCTGC 2 cut(s) 419, 1553
AccI GTMKAC 1 cut(s) 632
AciI CCGC 5 cut(s) 133, 466, 566, 745, 1296
AclWI GGATC 2 cut(s) 1008, 1021
AcoI YGGCCR 2 cut(s) 3, 660
AcsI RAATTY 2 cut(s) 824, 1615
AcuI CTGAAG 1 cut(s) 682
AfaI GTAC 4 cut(s) 292, 894, 1405, 1567
AfeI AGCGCT 1 cut(s) 850
AfiI CCNNNNNNNGG 4 cut(s) 860, 952, 991, 1332
AflIII ACRYGT 2 cut(s) 493, 1400
AgsI TTSAA 6 cut(s) 161, 400, 1063, 1389, 1615, 1622
AjnI CCWGG 2 cut(s) 662, 1021
AleI CACNNNNGTG 1 cut(s) 1421
AluBI AGCT 6 cut(s) 164, 507, 798, 1033, 1239, 1349
AluI AGCT 6 cut(s) 164, 507, 798, 1033, 1239, 1349
AlwI GGATC 2 cut(s) 1008, 1021
AlwNI CAGNNNCTG 2 cut(s) 1469, 1631
Ama87I CYCGRG 1 cut(s) 1041
Aor51HI AGCGCT 1 cut(s) 850
AoxI GGCC 6 cut(s) 3, 33, 660, 1037, 1293, 1459
ApeKI GCWGC 5 cut(s) 105, 507, 967, 1236, 1346
ApoI RAATTY 2 cut(s) 824, 1615
Asp700I GAANNNNTTC 1 cut(s) 1632
AspLEI GCGC 3 cut(s) 27, 105, 851
AspS9I GGNCC 1 cut(s) 1460
AsuHPI GGTGA 1 cut(s) 697
AvaI CYCGRG 1 cut(s) 1041
BaeI ACNNNNGTAYC 2 cut(s) 480, 513
BalI TGGCCA 1 cut(s) 662
BamHI GGATCC 1 cut(s) 1013
BarI GAAGNNNNNNTAC 2 cut(s) 616, 648
BbvCI CCTCAGC 1 cut(s) 1034
BbvI GCAGC 5 cut(s) 117, 494, 979, 1248, 1358
BccI CCATC 4 cut(s) 265, 1270, 1451, 1591
BceAI ACGGC 2 cut(s) 237, 623
BciT130I CCWGG 2 cut(s) 664, 1023
BciVI GTATCC 1 cut(s) 508
BfaI CTAG 4 cut(s) 941, 1350, 1367, 1512
BfmI CTRYAG 2 cut(s) 502, 1240
BfoI RGCGCY 1 cut(s) 852
BfuAI ACCTGC 2 cut(s) 419, 1553
BfuI GTATCC 1 cut(s) 508
BisI GCNGC 7 cut(s) 106, 508, 567, 968, 1237, 1296, 1347
BlsI GCNGC 7 cut(s) 107, 509, 568, 969, 1238, 1297, 1348
Bme1390I CCNGG 2 cut(s) 664, 1023
BmeT110I CYCGRG 1 cut(s) 1041
BmgT120I GGNCC 1 cut(s) 1460
BmiI GGNNCC 1 cut(s) 1015
BmrFI CCNGG 2 cut(s) 664, 1023
BmrI ACTGGG 1 cut(s) 1529
BmsI GCATC 6 cut(s) 180, 553, 1462, 1598, 1610, 1655
BmuI ACTGGG 1 cut(s) 1529
Bpu10I CCTNAGC 1 cut(s) 1034
BpuEI CTTGAG 2 cut(s) 214, 473
BsaBI GATNNNNATC 1 cut(s) 923
BsaJI CCNNGG 2 cut(s) 1263, 1637
Bsc4I CCNNNNNNNGG 4 cut(s) 860, 952, 991, 1332
Bse1I ACTGG 3 cut(s) 663, 1505, 1535
Bse8I GATNNNNATC 1 cut(s) 923
BseBI CCWGG 2 cut(s) 664, 1023
BseDI CCNNGG 2 cut(s) 1263, 1637
BseGI GGATG 1 cut(s) 568
BseJI GATNNNNATC 1 cut(s) 923
BseLI CCNNNNNNNGG 4 cut(s) 860, 952, 991, 1332
BseMII CTCAG 3 cut(s) 324, 1025, 1286
BseNI ACTGG 3 cut(s) 663, 1505, 1535
BseXI GCAGC 5 cut(s) 117, 494, 979, 1248, 1358
BseYI CCCAGC 2 cut(s) 1220, 1462
BsgI GTGCAG 1 cut(s) 1255
BshFI GGCC 6 cut(s) 5, 35, 662, 1039, 1295, 1461
BsiHKCI CYCGRG 1 cut(s) 1041
BslI CCNNNNNNNGG 4 cut(s) 860, 952, 991, 1332
BsmI GAATGC 6 cut(s) 108, 252, 406, 712, 1003, 1117
BsnI GGCC 6 cut(s) 5, 35, 662, 1039, 1295, 1461
BsoBI CYCGRG 1 cut(s) 1041
Bsp1407I TGTACA 3 cut(s) 290, 892, 1403
Bsp143I GATC 4 cut(s) 809, 1013, 1048, 1441
BspACI CCGC 5 cut(s) 133, 466, 566, 745, 1296
BspANI GGCC 6 cut(s) 5, 35, 662, 1039, 1295, 1461
BspCNI CTCAG 3 cut(s) 325, 1026, 1285
BspHI TCATGA 1 cut(s) 759
BspLI GGNNCC 1 cut(s) 1015
BspMI ACCTGC 2 cut(s) 419, 1553
BspPI GGATC 2 cut(s) 1008, 1021
BsrGI TGTACA 3 cut(s) 290, 892, 1403
BsrI ACTGG 3 cut(s) 663, 1505, 1535
BssECI CCNNGG 2 cut(s) 1263, 1637
BssMI GATC 4 cut(s) 809, 1013, 1048, 1441
BssT1I CCWWGG 2 cut(s) 1263, 1637
Bst2UI CCWGG 2 cut(s) 664, 1023
Bst4CI ACNGT 7 cut(s) 64, 361, 545, 1257, 1420, 1526, 1633
Bst6I CTCTTC 3 cut(s) 177, 216, 813
BstAPI GCANNNNNTGC 2 cut(s) 986, 1472
BstAUI TGTACA 3 cut(s) 290, 892, 1403
BstC8I GCNNGC 1 cut(s) 525
BstDEI CTNAG 4 cut(s) 324, 333, 1034, 1272
BstF5I GGATG 1 cut(s) 568
BstH2I RGCGCY 1 cut(s) 852
BstHHI GCGC 3 cut(s) 27, 105, 851
BstKTI GATC 4 cut(s) 812, 1016, 1051, 1444
BstMBI GATC 4 cut(s) 809, 1013, 1048, 1441
BstMWI GCNNNNNNNGC 6 cut(s) 11, 100, 709, 986, 1242, 1472
BstNI CCWGG 2 cut(s) 664, 1023
BstNSI RCATGY 3 cut(s) 497, 974, 1404
BstSCI CCNGG 2 cut(s) 662, 1021
BstSFI CTRYAG 2 cut(s) 502, 1240
BstV1I GCAGC 5 cut(s) 117, 494, 979, 1248, 1358
BstX2I RGATCY 1 cut(s) 1013
BstYI RGATCY 1 cut(s) 1013
BsuI GTATCC 1 cut(s) 508
BsuRI GGCC 6 cut(s) 5, 35, 662, 1039, 1295, 1461
BtsCI GGATG 1 cut(s) 568
BtsIMutI CAGTG 3 cut(s) 656, 723, 1253
BveI ACCTGC 2 cut(s) 419, 1553
Cac8I GCNNGC 1 cut(s) 525
CaiI CAGNNNCTG 2 cut(s) 1469, 1631
CciI TCATGA 1 cut(s) 759
CfoI GCGC 3 cut(s) 27, 105, 851
Cfr13I GGNCC 1 cut(s) 1460
Csp6I GTAC 4 cut(s) 291, 893, 1404, 1566
CspCI CAANNNNNGTGG 2 cut(s) 60, 95
CviQI GTAC 4 cut(s) 291, 893, 1404, 1566
DdeI CTNAG 4 cut(s) 324, 333, 1034, 1272
DpnI GATC 4 cut(s) 811, 1015, 1050, 1443
DpnII GATC 4 cut(s) 809, 1013, 1048, 1441
EaeI YGGCCR 2 cut(s) 3, 660
Eam1104I CTCTTC 3 cut(s) 177, 216, 813
EarI CTCTTC 3 cut(s) 177, 216, 813
Eco130I CCWWGG 2 cut(s) 1263, 1637
Eco147I AGGCCT 1 cut(s) 1039
Eco47III AGCGCT 1 cut(s) 850
Eco57I CTGAAG 1 cut(s) 682
Eco88I CYCGRG 1 cut(s) 1041
EcoRI GAATTC 1 cut(s) 824
EcoRII CCWGG 2 cut(s) 662, 1021
EcoT14I CCWWGG 2 cut(s) 1263, 1637
ErhI CCWWGG 2 cut(s) 1263, 1637
FblI GTMKAC 1 cut(s) 632
Fnu4HI GCNGC 7 cut(s) 106, 508, 567, 968, 1237, 1296, 1347
FokI GGATG 1 cut(s) 575
Fsp4HI GCNGC 7 cut(s) 106, 508, 567, 968, 1237, 1296, 1347
FspBI CTAG 4 cut(s) 941, 1350, 1367, 1512
GlaI GCGC 3 cut(s) 26, 104, 850
GluI GCNGC 7 cut(s) 106, 508, 567, 968, 1237, 1296, 1347
GsaI CCCAGC 2 cut(s) 1224, 1466
HaeII RGCGCY 1 cut(s) 852
HaeIII GGCC 6 cut(s) 5, 35, 662, 1039, 1295, 1461
HhaI GCGC 3 cut(s) 27, 105, 851
Hin6I GCGC 3 cut(s) 25, 103, 849
HinP1I GCGC 3 cut(s) 25, 103, 849
HincII GTYRAC 1 cut(s) 69
HindII GTYRAC 1 cut(s) 69
HinfI GANTC 6 cut(s) 538, 587, 763, 805, 1180, 1385
HphI GGTGA 1 cut(s) 697
Hpy166II GTNNAC 4 cut(s) 69, 357, 633, 1568
Hpy188I TCNGA 3 cut(s) 334, 1179, 1275
Hpy188III TCNNGA 8 cut(s) 452, 760, 916, 1011, 1043, 1063, 1108, 1445
Hpy8I GTNNAC 4 cut(s) 69, 357, 633, 1568
HpyAV CCTTC 6 cut(s) 172, 618, 782, 956, 1356, 1583
HpyCH4III ACNGT 7 cut(s) 64, 361, 545, 1257, 1420, 1526, 1633
HpyCH4IV ACGT 1 cut(s) 374
HpyF10VI GCNNNNNNNGC 6 cut(s) 11, 100, 709, 986, 1242, 1472
HpyF3I CTNAG 4 cut(s) 324, 333, 1034, 1272
HpySE526I ACGT 1 cut(s) 374
HspAI GCGC 3 cut(s) 25, 103, 849
Kzo9I GATC 4 cut(s) 809, 1013, 1048, 1441
LmnI GCTCC 3 cut(s) 100, 856, 1025
Lsp1109I GCAGC 5 cut(s) 117, 494, 979, 1248, 1358
LweI GCATC 6 cut(s) 180, 553, 1462, 1598, 1610, 1655
MaeI CTAG 4 cut(s) 941, 1350, 1367, 1512
MaeII ACGT 1 cut(s) 374
MaeIII GTNAC 1 cut(s) 1267
MalI GATC 4 cut(s) 811, 1015, 1050, 1443
MboI GATC 4 cut(s) 809, 1013, 1048, 1441
MboII GAAGA 8 cut(s) 20, 194, 203, 323, 547, 688, 830, 1568
MflI RGATCY 1 cut(s) 1013
MlsI TGGCCA 1 cut(s) 662
MluCI AATT 7 cut(s) 72, 704, 824, 933, 1226, 1285, 1615
MluNI TGGCCA 1 cut(s) 662
MmeI TCCRAC 1 cut(s) 527
Mox20I TGGCCA 1 cut(s) 662
MroXI GAANNNNTTC 1 cut(s) 1632
MscI TGGCCA 1 cut(s) 662
MseI TTAA 6 cut(s) 119, 389, 512, 677, 737, 888
MslI CAYNNNNRTG 2 cut(s) 46, 1421
Msp20I TGGCCA 1 cut(s) 662
MspA1I CMGCKG 1 cut(s) 507
MspR9I CCNGG 2 cut(s) 664, 1023
Mva1269I GAATGC 6 cut(s) 108, 252, 406, 712, 1003, 1117
MvaI CCWGG 2 cut(s) 664, 1023
MwoI GCNNNNNNNGC 6 cut(s) 11, 100, 709, 986, 1242, 1472
NdeII GATC 4 cut(s) 809, 1013, 1048, 1441
NlaIV GGNNCC 1 cut(s) 1015
NspI RCATGY 3 cut(s) 497, 974, 1404
OliI CACNNNNGTG 1 cut(s) 1421
PaeR7I CTCGAG 1 cut(s) 1041
PagI TCATGA 1 cut(s) 759
PceI AGGCCT 1 cut(s) 1039
PciI ACATGT 2 cut(s) 493, 1400
PctI GAATGC 6 cut(s) 108, 252, 406, 712, 1003, 1117
PdmI GAANNNNTTC 1 cut(s) 1632
PfeI GAWTC 6 cut(s) 538, 587, 763, 805, 1180, 1385
PfoI TCCNGGA 1 cut(s) 1021
PkrI GCNGC 7 cut(s) 107, 509, 568, 969, 1238, 1297, 1348
PscI ACATGT 2 cut(s) 493, 1400
PsiI TTATAA 1 cut(s) 1290
Psp6I CCWGG 2 cut(s) 662, 1021
PspFI CCCAGC 2 cut(s) 1220, 1462
PspGI CCWGG 2 cut(s) 662, 1021
PspN4I GGNNCC 1 cut(s) 1015
PspPI GGNCC 1 cut(s) 1460
PstNI CAGNNNCTG 2 cut(s) 1469, 1631
PsuI RGATCY 1 cut(s) 1013
PvuII CAGCTG 1 cut(s) 507
RsaI GTAC 4 cut(s) 292, 894, 1405, 1567
RsaNI GTAC 4 cut(s) 291, 893, 1404, 1566
RseI CAYNNNNRTG 2 cut(s) 46, 1421
SaqAI TTAA 6 cut(s) 119, 389, 512, 677, 737, 888
SatI GCNGC 7 cut(s) 106, 508, 567, 968, 1237, 1296, 1347
Sau3AI GATC 4 cut(s) 809, 1013, 1048, 1441
Sau96I GGNCC 1 cut(s) 1460
ScrFI CCNGG 2 cut(s) 664, 1023
SfaNI GCATC 6 cut(s) 180, 553, 1462, 1598, 1610, 1655
SfcI CTRYAG 2 cut(s) 502, 1240
Sfr274I CTCGAG 1 cut(s) 1041
SlaI CTCGAG 1 cut(s) 1041
SmiMI CAYNNNNRTG 2 cut(s) 46, 1421
SmlI CTYRAG 3 cut(s) 229, 452, 1041
SmoI CTYRAG 3 cut(s) 229, 452, 1041
Sse9I AATT 7 cut(s) 72, 704, 824, 933, 1226, 1285, 1615
SseBI AGGCCT 1 cut(s) 1039
SsiI CCGC 5 cut(s) 133, 466, 566, 745, 1296
SspI AATATT 1 cut(s) 247
SspMI CTAG 4 cut(s) 941, 1350, 1367, 1512
StuI AGGCCT 1 cut(s) 1039
StyD4I CCNGG 2 cut(s) 662, 1021
StyI CCWWGG 2 cut(s) 1263, 1637
TaaI ACNGT 7 cut(s) 64, 361, 545, 1257, 1420, 1526, 1633
TaiI ACGT 1 cut(s) 377
TaqI TCGA 3 cut(s) 808, 1042, 1455
TasI AATT 7 cut(s) 72, 704, 824, 933, 1226, 1285, 1615
TatI WGTACW 3 cut(s) 290, 892, 1403
TauI GCSGC 2 cut(s) 569, 1298
TfiI GAWTC 6 cut(s) 538, 587, 763, 805, 1180, 1385
Tru1I TTAA 6 cut(s) 119, 389, 512, 677, 737, 888
Tru9I TTAA 6 cut(s) 119, 389, 512, 677, 737, 888
TscAI CASTG 3 cut(s) 663, 723, 1260
TseI GCWGC 5 cut(s) 105, 507, 967, 1236, 1346
TspRI CASTG 3 cut(s) 663, 723, 1260
XapI RAATTY 2 cut(s) 824, 1615
XceI RCATGY 3 cut(s) 497, 974, 1404
XcmI CCANNNNNNNNNTGG 1 cut(s) 836
XhoI CTCGAG 1 cut(s) 1041
XmiI GTMKAC 1 cut(s) 632
XmnI GAANNNNTTC 1 cut(s) 1632
XspI CTAG 4 cut(s) 941, 1350, 1367, 1512
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.