MD03G1224600.v1.1

May be involved in modulation of pathogen defense and leaf cell death

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0008350
Physical Location & Seq
Forward (+)
31033175 .. 31040432
7258 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1224600.v1.1.491

Sequence Viewer

Length: 1656 bp
ATGGCGGAAGACCAACAAGAAATGCGATCGTTGGCCTTGACTCCAACATGGTCTGTTGCTACTGTGTTGACAATTTTTGTGGCTGTTTCTCTGCTTGTCGAGCGCGGAATCCATAGGTTAAGCCATTGGTTGCGGAAAACTAATCGTAAGCCCTTGCTTGAAGCTGTGGAGAAAATGAAAGAAGAGTTGATGTTACTTGGGTTTATCTCTCTCCTTTTGACGGCTACCTCAAGCATGATAGCCAATATTTGCATTCCATCAAAGTTCTACAATAGCAGTTTTTCTCCATGCTCTAGGTCTGAGATTACTGAAGAAACTGAAAATAAACGTAAGCTGTGGATGTTTACTGTTCTGCCTCACTCATTCAGAAGAGTGTTAAATGAGTTGAATCGTAATTCCTGCAAAGAGGGTTATGAGCCATTTGTTTCATATCAAGGCCTTGAGCAGTTGCATCGCTTCATCTTTGTCATGGCAATAACACATATATCCTACAGCTGCTTAACGATGTTGCTGGCAATAGTGAAGATTCACAGCTGGAGAGTATGGGAGGAGGAGGCTCACATGGACCGACATGATTCATTGACTGAGATCACACGGCAGATGACAATGCGAAGGCAGTCTAGCTTTGTAAATCCATCAACTCCTGTTGTCAGGAATAACTTTTTTATCTGGGTGAGATGTTTCTTCCGGCAATTTGGGAATTCAGTGGTTCGTGCTGACTATTTAACACTCCGCATGGGCTTCATCACGAACCACAACCTTTCACCAAAGTATGATTTTCACAGCTATATGATTCGGTCTATGGAAGAGGAATTCCAAAGGATTGTTGGTGTGAGTGCCACACTCTGGGGATTTGTTGTTGCCTTCATGCTGTTTAATGTAAAAGGGTCTAATCTTTATTTCTGGATTGCAATCATTCCAATTACGCTAGTTCTTCTTGTGGGAATGAAGCTGCAGCATATTATTGCAACCCTAGCATTGGAGAATGCTGGTATAACTGGATCCCATGGAGGAGCAAAGCTGAGGCCTCGGGATGATCTTTTCTGGTTCAAGAAGCCCGAACTTTTGTTGTCCTTGATCCATTTTTGTTTATTCCAGAATGCATTTGAATTGGCTTCATTCTTTTGGTTTTGGTGGCAATTTGGGTATAATTCTTGCTTTATCCGAAGGCATTGGCTCGTGTATATAAGGCTGATTTTAGGGTTTGCCGGGCAGTTCCTGTGCAGCTACAGCACCTTGCCACTGTATGCCTTGGTTACTCAGATGGGAACGAATTATAAGGCGGCAATAATCCCACTACGGATAAGAGAAACAATTCATGGATGGGGAAAGGCAGCTAGGAGGAAAAGAAGGCTAGGCATATACACTGATGATTCAACCATACGCACAGAAACAAGCACAGTAGCATCACTTGAGGAAGATGATCATCAAATGATCGATTACCCTGCAGATGATGCTAATACGCATAATGGAATTGAGTTGCAACCGGTTTCTAGTTCTGTGTCCAGTCCTTGCCCAGTTGGTAACCAAACCTCAAGTAGGCCAGGTACACCCCTTCTTCGACCATCGGCTTCTGTTTCTTCTCAATCAGTTACATTGACTATTCAAACAGAAGGTATTCCAAGATCCGCTTCACTGCCAGCTAGAAGGGAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
Mlo

Protein Analysis

552

Amino Acids

63.38

Weight (kDa)

9.26

Isoelectric Point (pI)

52.66

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Mlo PF03094 9 - 460 7.8e-165 Mlo family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1278
AccB7I CCANNNNNTGG 1 cut(s) 846
AccII CGCG 1 cut(s) 105
AciI CCGC 6 cut(s) 5, 105, 133, 733, 1283, 1629
AclWI GGATC 4 cut(s) 996, 1009, 1072, 1620
AcsI RAATTY 2 cut(s) 700, 812
AcuI CTGAAG 1 cut(s) 330
AfaI GTAC 1 cut(s) 1549
AfiI CCNNNNNNNGG 4 cut(s) 220, 846, 979, 1539
AgeI ACCGGT 1 cut(s) 1486
AgsI TTSAA 6 cut(s) 161, 388, 1051, 1109, 1377, 1607
AjnI CCWGG 1 cut(s) 1543
AjuI GAANNNNNNNTTGG 2 cut(s) 1615, 1647
AlwI GGATC 4 cut(s) 996, 1009, 1072, 1620
AlwNI CAGNNNCTG 1 cut(s) 1219
Ama87I CYCGRG 1 cut(s) 1029
AoxI GGCC 4 cut(s) 33, 436, 1025, 1541
ApeKI GCWGC 5 cut(s) 495, 952, 955, 1224, 1334
ApoI RAATTY 2 cut(s) 700, 812
AsiGI ACCGGT 1 cut(s) 1486
Asp700I GAANNNNTTC 2 cut(s) 1314, 1617
AspLEI GCGC 1 cut(s) 105
AspS9I GGNCC 1 cut(s) 565
AsuC2I CCSGG 1 cut(s) 1210
AsuHPI GGTGA 2 cut(s) 685, 756
AvaI CYCGRG 1 cut(s) 1029
AvaII GGWCC 1 cut(s) 565
BamHI GGATCC 1 cut(s) 1001
BauI CACGAG 1 cut(s) 1178
BbsI GAAGAC 1 cut(s) 15
BbvCI CCTCAGC 1 cut(s) 1022
BbvI GCAGC 5 cut(s) 482, 939, 967, 1236, 1346
BccI CCATC 5 cut(s) 265, 643, 1258, 1317, 1573
BceAI ACGGC 2 cut(s) 237, 611
BcgI CGANNNNNNTGC 4 cut(s) 434, 468, 1427, 1461
BciT130I CCWGG 1 cut(s) 1545
BclI TGATCA 1 cut(s) 1423
BcnI CCSGG 1 cut(s) 1210
BfaI CTAG 8 cut(s) 294, 621, 929, 974, 1338, 1355, 1494, 1644
BfmI CTRYAG 4 cut(s) 490, 953, 1228, 1446
BisI GCNGC 6 cut(s) 496, 953, 956, 1225, 1284, 1335
BlsI GCNGC 6 cut(s) 497, 954, 957, 1226, 1285, 1336
Bme1390I CCNGG 2 cut(s) 1210, 1545
Bme18I GGWCC 1 cut(s) 565
BmeT110I CYCGRG 1 cut(s) 1029
BmgT120I GGNCC 1 cut(s) 565
BmiI GGNNCC 1 cut(s) 1003
BmrFI CCNGG 2 cut(s) 1210, 1545
BmrI ACTGGG 1 cut(s) 1511
BmsI GCATC 3 cut(s) 460, 1415, 1444
BmuI ACTGGG 1 cut(s) 1511
BpiI GAAGAC 1 cut(s) 15
BpmI CTGGAG 1 cut(s) 556
Bpu10I CCTNAGC 1 cut(s) 1022
BpuEI CTTGAG 4 cut(s) 214, 461, 1433, 1519
BpuMI CCSGG 1 cut(s) 1210
Bsa29I ATCGAT 1 cut(s) 1437
BsaBI GATNNNNATC 2 cut(s) 911, 1425
BsaJI CCNNGG 3 cut(s) 1006, 1028, 1251
BsaWI WCCGGW 1 cut(s) 1486
Bsc4I CCNNNNNNNGG 4 cut(s) 220, 846, 979, 1539
Bse118I RCCGGY 1 cut(s) 1486
Bse1I ACTGG 3 cut(s) 1003, 1506, 1517
Bse8I GATNNNNATC 2 cut(s) 911, 1425
BseBI CCWGG 1 cut(s) 1545
BseCI ATCGAT 1 cut(s) 1437
BseDI CCNNGG 3 cut(s) 1006, 1028, 1251
BseGI GGATG 3 cut(s) 345, 1039, 1328
BseJI GATNNNNATC 2 cut(s) 911, 1425
BseLI CCNNNNNNNGG 4 cut(s) 220, 846, 979, 1539
BseMII CTCAG 4 cut(s) 291, 576, 1013, 1274
BseNI ACTGG 3 cut(s) 1003, 1506, 1517
BseRI GAGGAG 3 cut(s) 563, 566, 1026
BseXI GCAGC 5 cut(s) 482, 939, 967, 1236, 1346
BsgI GTGCAG 1 cut(s) 1243
Bsh1236I CGCG 1 cut(s) 105
Bsh1285I CGRYCG 1 cut(s) 29
BshFI GGCC 4 cut(s) 35, 438, 1027, 1543
BshTI ACCGGT 1 cut(s) 1486
BshVI ATCGAT 1 cut(s) 1437
BsiEI CGRYCG 1 cut(s) 29
BsiHKCI CYCGRG 1 cut(s) 1029
BsiSI CCGG 3 cut(s) 688, 1209, 1487
BslI CCNNNNNNNGG 4 cut(s) 220, 846, 979, 1539
BsmI GAATGC 3 cut(s) 252, 991, 1105
BsnI GGCC 4 cut(s) 35, 438, 1027, 1543
BsoBI CYCGRG 1 cut(s) 1029
Bsp143I GATC 8 cut(s) 26, 588, 1001, 1036, 1077, 1423, 1434, 1625
Bsp19I CCATGG 1 cut(s) 1006
BspACI CCGC 6 cut(s) 5, 105, 133, 733, 1283, 1629
BspANI GGCC 4 cut(s) 35, 438, 1027, 1543
BspCNI CTCAG 4 cut(s) 292, 577, 1014, 1273
BspDI ATCGAT 1 cut(s) 1437
BspFNI CGCG 1 cut(s) 105
BspLI GGNNCC 1 cut(s) 1003
BspMAI CTGCAG 2 cut(s) 957, 1450
BspPI GGATC 4 cut(s) 996, 1009, 1072, 1620
BsrFI RCCGGY 1 cut(s) 1486
BsrI ACTGG 3 cut(s) 1003, 1506, 1517
BssAI RCCGGY 1 cut(s) 1486
BssECI CCNNGG 3 cut(s) 1006, 1028, 1251
BssMI GATC 8 cut(s) 26, 588, 1001, 1036, 1077, 1423, 1434, 1625
BssSI CACGAG 1 cut(s) 1178
BssT1I CCWWGG 2 cut(s) 1006, 1251
Bst2BI CACGAG 1 cut(s) 1178
Bst2UI CCWGG 1 cut(s) 1545
Bst4CI ACNGT 4 cut(s) 64, 349, 1245, 1402
Bst6I CTCTTC 3 cut(s) 177, 364, 801
BstAPI GCANNNNNTGC 1 cut(s) 1454
BstC8I GCNNGC 2 cut(s) 513, 1641
BstDEI CTNAG 4 cut(s) 300, 585, 1022, 1260
BstDSI CCRYGG 1 cut(s) 1006
BstEII GGTNACC 1 cut(s) 1523
BstENI CCTNNNNNAGG 1 cut(s) 1537
BstF5I GGATG 3 cut(s) 345, 1039, 1328
BstFNI CGCG 1 cut(s) 105
BstHHI GCGC 1 cut(s) 105
BstKTI GATC 8 cut(s) 29, 591, 1004, 1039, 1080, 1426, 1437, 1628
BstMBI GATC 8 cut(s) 26, 588, 1001, 1036, 1077, 1423, 1434, 1625
BstMCI CGRYCG 1 cut(s) 29
BstMWI GCNNNNNNNGC 4 cut(s) 100, 974, 1230, 1454
BstNI CCWGG 1 cut(s) 1545
BstPI GGTNACC 1 cut(s) 1523
BstSCI CCNGG 2 cut(s) 1208, 1543
BstSFI CTRYAG 4 cut(s) 490, 953, 1228, 1446
BstUI CGCG 1 cut(s) 105
BstV1I GCAGC 5 cut(s) 482, 939, 967, 1236, 1346
BstV2I GAAGAC 1 cut(s) 15
BstX2I RGATCY 2 cut(s) 1001, 1625
BstYI RGATCY 2 cut(s) 1001, 1625
Bsu15I ATCGAT 1 cut(s) 1437
BsuRI GGCC 4 cut(s) 35, 438, 1027, 1543
BsuTUI ATCGAT 1 cut(s) 1437
BtgI CCRYGG 1 cut(s) 1006
BtgZI GCGATG 1 cut(s) 437
BtsCI GGATG 3 cut(s) 345, 1039, 1328
BtsI GCAGTG 1 cut(s) 1634
BtsIMutI CAGTG 4 cut(s) 711, 1241, 1365, 1634
Cac8I GCNNGC 2 cut(s) 513, 1641
CaiI CAGNNNCTG 1 cut(s) 1219
CfoI GCGC 1 cut(s) 105
Cfr10I RCCGGY 1 cut(s) 1486
Cfr13I GGNCC 1 cut(s) 565
ClaI ATCGAT 1 cut(s) 1437
Csp6I GTAC 1 cut(s) 1548
CspAI ACCGGT 1 cut(s) 1486
CspCI CAANNNNNGTGG 2 cut(s) 60, 95
CviQI GTAC 1 cut(s) 1548
DdeI CTNAG 4 cut(s) 300, 585, 1022, 1260
DpnI GATC 8 cut(s) 28, 590, 1003, 1038, 1079, 1425, 1436, 1627
DpnII GATC 8 cut(s) 26, 588, 1001, 1036, 1077, 1423, 1434, 1625
Eam1104I CTCTTC 3 cut(s) 177, 364, 801
EarI CTCTTC 3 cut(s) 177, 364, 801
EciI GGCGGA 1 cut(s) 20
Eco130I CCWWGG 2 cut(s) 1006, 1251
Eco147I AGGCCT 2 cut(s) 438, 1027
Eco47I GGWCC 1 cut(s) 565
Eco57I CTGAAG 1 cut(s) 330
Eco88I CYCGRG 1 cut(s) 1029
Eco91I GGTNACC 1 cut(s) 1523
EcoNI CCTNNNNNAGG 1 cut(s) 1537
EcoO65I GGTNACC 1 cut(s) 1523
EcoRI GAATTC 2 cut(s) 700, 812
EcoRII CCWGG 1 cut(s) 1543
EcoT14I CCWWGG 2 cut(s) 1006, 1251
EcoT22I ATGCAT 1 cut(s) 1105
ErhI CCWWGG 2 cut(s) 1006, 1251
FalI AAGNNNNNCTT 2 cut(s) 1615, 1647
FbaI TGATCA 1 cut(s) 1423
Fnu4HI GCNGC 6 cut(s) 496, 953, 956, 1225, 1284, 1335
FokI GGATG 3 cut(s) 352, 1046, 1335
Fsp4HI GCNGC 6 cut(s) 496, 953, 956, 1225, 1284, 1335
FspBI CTAG 8 cut(s) 294, 621, 929, 974, 1338, 1355, 1494, 1644
GlaI GCGC 1 cut(s) 104
GluI GCNGC 6 cut(s) 496, 953, 956, 1225, 1284, 1335
GsuI CTGGAG 1 cut(s) 556
HaeIII GGCC 4 cut(s) 35, 438, 1027, 1543
HapII CCGG 3 cut(s) 688, 1209, 1487
HhaI GCGC 1 cut(s) 105
Hin6I GCGC 1 cut(s) 103
HinP1I GCGC 1 cut(s) 103
HincII GTYRAC 1 cut(s) 69
HindII GTYRAC 1 cut(s) 69
HinfI GANTC 7 cut(s) 40, 108, 388, 526, 575, 793, 1373
HpaII CCGG 3 cut(s) 688, 1209, 1487
HphI GGTGA 2 cut(s) 685, 756
Hpy166II GTNNAC 3 cut(s) 69, 345, 1550
Hpy188I TCNGA 4 cut(s) 301, 368, 1166, 1263
Hpy188III TCNNGA 6 cut(s) 652, 748, 904, 1031, 1051, 1096
Hpy8I GTNNAC 3 cut(s) 69, 345, 1550
HpyAV CCTTC 7 cut(s) 606, 874, 1161, 1344, 1565, 1607, 1641
HpyCH4III ACNGT 4 cut(s) 64, 349, 1245, 1402
HpyCH4IV ACGT 1 cut(s) 328
HpyF10VI GCNNNNNNNGC 4 cut(s) 100, 974, 1230, 1454
HpyF3I CTNAG 4 cut(s) 300, 585, 1022, 1260
HpySE526I ACGT 1 cut(s) 328
HspAI GCGC 1 cut(s) 103
Ksp22I TGATCA 1 cut(s) 1423
Kzo9I GATC 8 cut(s) 26, 588, 1001, 1036, 1077, 1423, 1434, 1625
LmnI GCTCC 1 cut(s) 1013
Lsp1109I GCAGC 5 cut(s) 482, 939, 967, 1236, 1346
LweI GCATC 3 cut(s) 460, 1415, 1444
MaeI CTAG 8 cut(s) 294, 621, 929, 974, 1338, 1355, 1494, 1644
MaeII ACGT 1 cut(s) 328
MaeIII GTNAC 4 cut(s) 192, 1255, 1523, 1591
MalI GATC 8 cut(s) 28, 590, 1003, 1038, 1079, 1425, 1436, 1627
MboI GATC 8 cut(s) 26, 588, 1001, 1036, 1077, 1423, 1434, 1625
MflI RGATCY 2 cut(s) 1001, 1625
MlyI GAGTC 1 cut(s) 34
MmeI TCCRAC 1 cut(s) 68
Mph1103I ATGCAT 1 cut(s) 1105
MroXI GAANNNNTTC 2 cut(s) 1314, 1617
MseI TTAA 5 cut(s) 119, 377, 500, 725, 876
MspA1I CMGCKG 2 cut(s) 495, 534
MspI CCGG 3 cut(s) 688, 1209, 1487
MspR9I CCNGG 2 cut(s) 1210, 1545
Mva1269I GAATGC 3 cut(s) 252, 991, 1105
MvaI CCWGG 1 cut(s) 1545
MvnI CGCG 1 cut(s) 105
MwoI GCNNNNNNNGC 4 cut(s) 100, 974, 1230, 1454
NciI CCSGG 1 cut(s) 1210
NcoI CCATGG 1 cut(s) 1006
NdeII GATC 8 cut(s) 26, 588, 1001, 1036, 1077, 1423, 1434, 1625
NlaIV GGNNCC 1 cut(s) 1003
NsiI ATGCAT 1 cut(s) 1105
PceI AGGCCT 2 cut(s) 438, 1027
PctI GAATGC 3 cut(s) 252, 991, 1105
PdmI GAANNNNTTC 2 cut(s) 1314, 1617
PfeI GAWTC 6 cut(s) 108, 388, 526, 575, 793, 1373
PflMI CCANNNNNTGG 1 cut(s) 846
PinAI ACCGGT 1 cut(s) 1486
PkrI GCNGC 6 cut(s) 497, 954, 957, 1226, 1285, 1336
Ple19I CGATCG 1 cut(s) 29
PleI GAGTC 1 cut(s) 34
PpsI GAGTC 1 cut(s) 34
PsiI TTATAA 1 cut(s) 1278
Psp6I CCWGG 1 cut(s) 1543
PspEI GGTNACC 1 cut(s) 1523
PspGI CCWGG 1 cut(s) 1543
PspN4I GGNNCC 1 cut(s) 1003
PspPI GGNCC 1 cut(s) 565
PstI CTGCAG 2 cut(s) 957, 1450
PstNI CAGNNNCTG 1 cut(s) 1219
PsuI RGATCY 2 cut(s) 1001, 1625
PvuI CGATCG 1 cut(s) 29
PvuII CAGCTG 2 cut(s) 495, 534
RsaI GTAC 1 cut(s) 1549
RsaNI GTAC 1 cut(s) 1548
SaqAI TTAA 5 cut(s) 119, 377, 500, 725, 876
SatI GCNGC 6 cut(s) 496, 953, 956, 1225, 1284, 1335
Sau3AI GATC 8 cut(s) 26, 588, 1001, 1036, 1077, 1423, 1434, 1625
Sau96I GGNCC 1 cut(s) 565
SchI GAGTC 1 cut(s) 34
ScrFI CCNGG 2 cut(s) 1210, 1545
SfaNI GCATC 3 cut(s) 460, 1415, 1444
SfcI CTRYAG 4 cut(s) 490, 953, 1228, 1446
SinI GGWCC 1 cut(s) 565
SmlI CTYRAG 4 cut(s) 229, 440, 1412, 1534
SmoI CTYRAG 4 cut(s) 229, 440, 1412, 1534
SseBI AGGCCT 2 cut(s) 438, 1027
SsiI CCGC 6 cut(s) 5, 105, 133, 733, 1283, 1629
SspI AATATT 1 cut(s) 247
SspMI CTAG 8 cut(s) 294, 621, 929, 974, 1338, 1355, 1494, 1644
StuI AGGCCT 2 cut(s) 438, 1027
StyD4I CCNGG 2 cut(s) 1208, 1543
StyI CCWWGG 2 cut(s) 1006, 1251
TaaI ACNGT 4 cut(s) 64, 349, 1245, 1402
TaiI ACGT 1 cut(s) 331
TaqI TCGA 3 cut(s) 99, 1437, 1561
TaqII GACCGA 2 cut(s) 582, 786
TauI GCSGC 1 cut(s) 1286
TfiI GAWTC 6 cut(s) 108, 388, 526, 575, 793, 1373
Tru1I TTAA 5 cut(s) 119, 377, 500, 725, 876
Tru9I TTAA 5 cut(s) 119, 377, 500, 725, 876
TscAI CASTG 4 cut(s) 711, 1248, 1372, 1641
TseI GCWGC 5 cut(s) 495, 952, 955, 1224, 1334
TspDTI ATGAA 9 cut(s) 191, 417, 448, 567, 733, 856, 962, 1107, 1307
TspGWI ACGGA 1 cut(s) 1315
TspRI CASTG 4 cut(s) 711, 1248, 1372, 1641
Van91I CCANNNNNTGG 1 cut(s) 846
VpaK11BI GGWCC 1 cut(s) 565
XagI CCTNNNNNAGG 1 cut(s) 1537
XapI RAATTY 2 cut(s) 700, 812
XcmI CCANNNNNNNNNTGG 1 cut(s) 824
XmnI GAANNNNTTC 2 cut(s) 1314, 1617
XspI CTAG 8 cut(s) 294, 621, 929, 974, 1338, 1355, 1494, 1644
Zsp2I ATGCAT 1 cut(s) 1105
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.