AT5G54140

Peptidase family M20/M25/M40

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010280
Physical Location & Seq
Forward (+)
21965667 .. 21968061
2395 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G54140.1

Sequence Viewer

Length: 1287 bp
ATGGCTAATTCCTCAATCGTAGCTCTTCTTCTTCTCTTTGTTATCGCTAGTTCCGTCAATGGAGGAGATCAAGAGTATCCAAATCAATATCTAACAGAAGCTTTAGGTGATAAAGAATGGTTAGTGTCTGTAAGAAGACAAATCCATGAAAACCCAGAACTCCTTTTCGAGTTACATAAAACAAGTGCTCTGATTCGTCGCGAGCTTGATGAATTAGGGGTTTCTTATTCTTACCCGGTTGCGAAAACCGGGATCGTAGCTCAAATCGGATCCGGTTATCCTCCGGTTGTTGCTCTCCGTGCTGATATGGATGCTCTTCCCTTACAAGAACTTGTTGAGTGGGATCATAAGAGCAAAATCGATGGAAAAATGCATGCTTGTGGTCATGATTCTCATACCACTATGCTCCTTGGTGCTGCTAAATTGCTTAGCAAACGCAAACGTATGCTCAATGGAACAGTGAGGCTTCTTTTTCAACCAGCAGAAGAAGGTGGTGCAGGTGCTTTTCATATGATAAAAGAAGGTGCTTTGGGTGATTCTGAAGCAATATTTGGGATGCATGTTCATACTGGCTTACCTACAGGAGAATTAGCAACAATCTCGGGTCCTGCTTTGGCATCCACAAGCATTTTCAGTGTAAGAATGAGTGGTAAATCACCCGCTTCTTCGGAGACCTATTCTTGTGTTGATCCGGTGTTGGCTGCATCATCTACCATCTTAGCATTGCAACTTATTATCTCCAGAGAAGTAGATCCTCTCTTAAGCCATGTGCTCTCTGTTACATTTATGAAAAGTGGCGGTTCTGAGTTTGATGTGATTCCGGCATATGTAGAATTTGGGGGAACTTTGAGGAGTCTTACAACCAATGGCATCAACTGGTTGATAAAGCGGTTGAAAGAGGTGGTTGAAGGACAAGCTGAAGTACAAAGATGCAAAGCGGATATAGATATGCATGAAGACGATCATCCTATGTATCCAGCAACAGTGAACGATCACAAGCTACACGAATTCACTGAAAAGGTTTTGAAACTCTTGCTTGGACCCGAGAAGGTGAAACCGGCTAACAAGGTAATGGCGGGTGAGGATTTTGCTTTCTATCAACAAAAAATACCCGGTTACTACATAGGAATCGGTATAAGAAACGAAGAAATCGGTTCTGTGCGTTCGGTTCATTCGCCTTATTTCTTCCTCGACGAGAATGTGCTTCCCATTGGATCAGCAACGTTTGCTGCCTTAGCTGAAATGTATCTACAAGAGCATCAGAATCAAACTAAGTCTGGAGATTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009850 GO:0009987 GO:0010178 GO:0010817 GO:0016787 GO:0016810 GO:0042445 GO:0044237 GO:0065007 GO:0065008
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M20_dimer Peptidase_M20

Protein Analysis

428

Amino Acids

46.79

Weight (kDa)

5.6

Isoelectric Point (pI)

44.73

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 98 - 414 6.2e-43 Peptidase family M20/M25/M40
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 488
Acc36I ACCTGC 1 cut(s) 488
AccII CGCG 1 cut(s) 201
AciI CCGC 5 cut(s) 660, 798, 889, 938, 1076
AclI AACGTT 1 cut(s) 1223
AclWI GGATC 7 cut(s) 260, 264, 277, 351, 683, 746, 1222
AcsI RAATTY 2 cut(s) 833, 1007
AcuI CTGAAG 2 cut(s) 561, 939
AfaI GTAC 1 cut(s) 924
AflII CTTAAG 1 cut(s) 760
AgsI TTSAA 4 cut(s) 476, 895, 908, 1027
AjuI GAANNNNNNNTTGG 2 cut(s) 534, 566
AluBI AGCT 7 cut(s) 23, 101, 205, 260, 917, 1000, 1238
AluI AGCT 7 cut(s) 23, 101, 205, 260, 917, 1000, 1238
Alw21I GWGCWC 2 cut(s) 190, 774
Alw26I GTCTC 1 cut(s) 665
AlwI GGATC 7 cut(s) 260, 264, 277, 351, 683, 746, 1222
Ama87I CYCGRG 2 cut(s) 601, 1043
ApeKI GCWGC 3 cut(s) 416, 701, 1229
ApoI RAATTY 2 cut(s) 833, 1007
AspS9I GGNCC 2 cut(s) 605, 1040
AsuC2I CCSGG 3 cut(s) 236, 250, 1113
AsuHPI GGTGA 5 cut(s) 119, 545, 648, 1063, 1091
AvaI CYCGRG 2 cut(s) 601, 1043
AvaII GGWCC 2 cut(s) 605, 1040
BamHI GGATCC 1 cut(s) 269
BarI GAAGNNNNNNTAC 2 cut(s) 12, 44
BbsI GAAGAC 2 cut(s) 142, 963
Bbv12I GWGCWC 2 cut(s) 190, 774
BbvI GCAGC 3 cut(s) 403, 688, 1216
BccI CCATC 2 cut(s) 356, 722
BcgI CGANNNNNNTGC 2 cut(s) 582, 616
BciVI GTATCC 2 cut(s) 87, 984
BcnI CCSGG 3 cut(s) 236, 250, 1113
BcoDI GTCTC 1 cut(s) 665
BfaI CTAG 1 cut(s) 48
BfmI CTRYAG 1 cut(s) 579
BfrI CTTAAG 1 cut(s) 760
BfuAI ACCTGC 1 cut(s) 488
BfuI GTATCC 2 cut(s) 87, 984
BisI GCNGC 3 cut(s) 417, 702, 1230
BlpI GCTNAGC 1 cut(s) 428
BlsI GCNGC 3 cut(s) 418, 703, 1231
Bme1390I CCNGG 3 cut(s) 236, 250, 1113
Bme18I GGWCC 2 cut(s) 605, 1040
BmeT110I CYCGRG 2 cut(s) 601, 1043
BmgT120I GGNCC 2 cut(s) 605, 1040
BmiI GGNNCC 3 cut(s) 271, 606, 1042
BmrFI CCNGG 3 cut(s) 236, 250, 1113
BmsI GCATC 7 cut(s) 301, 546, 626, 713, 879, 920, 1267
BpiI GAAGAC 2 cut(s) 142, 963
BpmI CTGGAG 1 cut(s) 724
Bpu10I CCTNAGC 1 cut(s) 1234
Bpu1102I GCTNAGC 1 cut(s) 428
BpuMI CCSGG 3 cut(s) 236, 250, 1113
Bsa29I ATCGAT 1 cut(s) 360
BsaI GGTCTC 1 cut(s) 665
BsaJI CCNNGG 1 cut(s) 409
BsaWI WCCGGW 3 cut(s) 272, 283, 691
Bse118I RCCGGY 1 cut(s) 1057
Bse1I ACTGG 2 cut(s) 574, 881
Bse3DI GCAATG 1 cut(s) 722
BseCI ATCGAT 1 cut(s) 360
BseDI CCNNGG 1 cut(s) 409
BseGI GGATG 4 cut(s) 316, 561, 617, 964
BseMI GCAATG 1 cut(s) 722
BseMII CTCAG 1 cut(s) 795
BseNI ACTGG 2 cut(s) 574, 881
BseRI GAGGAG 2 cut(s) 78, 865
BseXI GCAGC 3 cut(s) 403, 688, 1216
BsgI GTGCAG 1 cut(s) 516
Bsh1236I CGCG 1 cut(s) 201
BshVI ATCGAT 1 cut(s) 360
BsiHKAI GWGCWC 2 cut(s) 190, 774
BsiHKCI CYCGRG 2 cut(s) 601, 1043
BsiSI CCGG 8 cut(s) 236, 249, 273, 284, 692, 821, 1058, 1113
BsmAI GTCTC 1 cut(s) 665
Bso31I GGTCTC 1 cut(s) 665
BsoBI CYCGRG 2 cut(s) 601, 1043
Bsp1286I GDGCHC 2 cut(s) 190, 774
Bsp143I GATC 9 cut(s) 67, 252, 269, 343, 688, 751, 961, 991, 1214
Bsp1720I GCTNAGC 1 cut(s) 428
Bsp68I TCGCGA 1 cut(s) 201
BspACI CCGC 5 cut(s) 660, 798, 889, 938, 1076
BspCNI CTCAG 1 cut(s) 796
BspDI ATCGAT 1 cut(s) 360
BspFNI CGCG 1 cut(s) 201
BspHI TCATGA 1 cut(s) 385
BspLI GGNNCC 3 cut(s) 271, 606, 1042
BspMI ACCTGC 1 cut(s) 488
BspPI GGATC 7 cut(s) 260, 264, 277, 351, 683, 746, 1222
BspQI GCTCTTC 2 cut(s) 30, 321
BspTI CTTAAG 1 cut(s) 760
BspTNI GGTCTC 1 cut(s) 665
BsrDI GCAATG 1 cut(s) 722
BsrFI RCCGGY 1 cut(s) 1057
BsrI ACTGG 2 cut(s) 574, 881
BssAI RCCGGY 1 cut(s) 1057
BssECI CCNNGG 1 cut(s) 409
BssMI GATC 9 cut(s) 67, 252, 269, 343, 688, 751, 961, 991, 1214
BssT1I CCWWGG 1 cut(s) 409
Bst4CI ACNGT 2 cut(s) 460, 985
Bst6I CTCTTC 2 cut(s) 30, 321
BstAFI CTTAAG 1 cut(s) 760
BstAPI GCANNNNNTGC 1 cut(s) 1226
BstC8I GCNNGC 2 cut(s) 203, 375
BstDEI CTNAG 5 cut(s) 428, 718, 804, 1234, 1272
BstF5I GGATG 4 cut(s) 316, 561, 617, 964
BstFNI CGCG 1 cut(s) 201
BstKTI GATC 9 cut(s) 70, 255, 272, 346, 691, 754, 964, 994, 1217
BstMAI GTCTC 1 cut(s) 665
BstMBI GATC 9 cut(s) 67, 252, 269, 343, 688, 751, 961, 991, 1214
BstMWI GCNNNNNNNGC 3 cut(s) 299, 1226, 1235
BstNSI RCATGY 2 cut(s) 377, 563
BstSCI CCNGG 3 cut(s) 234, 248, 1111
BstSFI CTRYAG 1 cut(s) 579
BstUI CGCG 1 cut(s) 201
BstV1I GCAGC 3 cut(s) 403, 688, 1216
BstV2I GAAGAC 2 cut(s) 142, 963
BstX2I RGATCY 2 cut(s) 269, 751
BstYI RGATCY 2 cut(s) 269, 751
Bsu15I ATCGAT 1 cut(s) 360
BsuI GTATCC 2 cut(s) 87, 984
BsuTUI ATCGAT 1 cut(s) 360
BtsCI GGATG 4 cut(s) 316, 561, 617, 964
BtsIMutI CAGTG 4 cut(s) 465, 640, 990, 1011
BtuMI TCGCGA 1 cut(s) 201
BveI ACCTGC 1 cut(s) 488
Cac8I GCNNGC 2 cut(s) 203, 375
CciI TCATGA 1 cut(s) 385
Cfr10I RCCGGY 1 cut(s) 1057
Cfr13I GGNCC 2 cut(s) 605, 1040
ClaI ATCGAT 1 cut(s) 360
Csp6I GTAC 1 cut(s) 923
CviAII CATG 6 cut(s) 146, 374, 386, 560, 767, 953
CviQI GTAC 1 cut(s) 923
DdeI CTNAG 5 cut(s) 428, 718, 804, 1234, 1272
DpnI GATC 9 cut(s) 69, 254, 271, 345, 690, 753, 963, 993, 1216
DpnII GATC 9 cut(s) 67, 252, 269, 343, 688, 751, 961, 991, 1214
Eam1104I CTCTTC 2 cut(s) 30, 321
EarI CTCTTC 2 cut(s) 30, 321
Eco130I CCWWGG 1 cut(s) 409
Eco31I GGTCTC 1 cut(s) 665
Eco47I GGWCC 2 cut(s) 605, 1040
Eco57I CTGAAG 2 cut(s) 561, 939
Eco88I CYCGRG 2 cut(s) 601, 1043
EcoO109I RGGNCCY 1 cut(s) 605
EcoRI GAATTC 1 cut(s) 1007
EcoT14I CCWWGG 1 cut(s) 409
EcoT22I ATGCAT 3 cut(s) 375, 561, 954
ErhI CCWWGG 1 cut(s) 409
FaeI CATG 6 cut(s) 149, 377, 389, 563, 770, 956
FatI CATG 6 cut(s) 145, 373, 385, 559, 766, 952
FauI CCCGC 2 cut(s) 667, 1069
FauNDI CATATG 2 cut(s) 510, 826
Fnu4HI GCNGC 3 cut(s) 417, 702, 1230
FokI GGATG 4 cut(s) 323, 568, 604, 951
Fsp4HI GCNGC 3 cut(s) 417, 702, 1230
FspBI CTAG 1 cut(s) 48
GluI GCNGC 3 cut(s) 417, 702, 1230
GsuI CTGGAG 1 cut(s) 724
HapII CCGG 8 cut(s) 236, 249, 273, 284, 692, 821, 1058, 1113
Hin1II CATG 6 cut(s) 149, 377, 389, 563, 770, 956
HindIII AAGCTT 1 cut(s) 99
HinfI GANTC 7 cut(s) 193, 389, 536, 817, 853, 1128, 1264
HpaII CCGG 8 cut(s) 236, 249, 273, 284, 692, 821, 1058, 1113
HphI GGTGA 5 cut(s) 119, 545, 648, 1063, 1091
Hpy166II GTNNAC 1 cut(s) 988
Hpy188I TCNGA 6 cut(s) 192, 269, 541, 670, 805, 1263
Hpy188III TCNNGA 5 cut(s) 71, 200, 386, 741, 1278
Hpy8I GTNNAC 1 cut(s) 988
Hpy99I CGWCG 2 cut(s) 201, 1196
HpyAV CCTTC 4 cut(s) 482, 515, 902, 1042
HpyCH4III ACNGT 2 cut(s) 460, 985
HpyCH4IV ACGT 2 cut(s) 442, 1223
HpyCH4V TGCA 7 cut(s) 373, 497, 559, 704, 727, 933, 952
HpyF10VI GCNNNNNNNGC 3 cut(s) 299, 1226, 1235
HpyF3I CTNAG 5 cut(s) 428, 718, 804, 1234, 1272
HpySE526I ACGT 2 cut(s) 442, 1223
Hsp92II CATG 6 cut(s) 149, 377, 389, 563, 770, 956
Kzo9I GATC 9 cut(s) 67, 252, 269, 343, 688, 751, 961, 991, 1214
LguI GCTCTTC 2 cut(s) 30, 321
LmnI GCTCC 1 cut(s) 411
Lsp1109I GCAGC 3 cut(s) 403, 688, 1216
LweI GCATC 7 cut(s) 301, 546, 626, 713, 879, 920, 1267
MaeI CTAG 1 cut(s) 48
MaeII ACGT 2 cut(s) 442, 1223
MaeIII GTNAC 3 cut(s) 171, 778, 1115
MalI GATC 9 cut(s) 69, 254, 271, 345, 690, 753, 963, 993, 1216
MboI GATC 9 cut(s) 67, 252, 269, 343, 688, 751, 961, 991, 1214
MflI RGATCY 2 cut(s) 269, 751
MhlI GDGCHC 2 cut(s) 190, 774
MluCI AATT 6 cut(s) 7, 212, 422, 587, 833, 1007
MlyI GAGTC 1 cut(s) 862
MnlI CCTC 9 cut(s) 22, 56, 291, 456, 765, 843, 892, 1075, 1199
Mph1103I ATGCAT 3 cut(s) 375, 561, 954
MseI TTAA 1 cut(s) 761
MslI CAYNNNNRTG 1 cut(s) 378
MspCI CTTAAG 1 cut(s) 760
MspI CCGG 8 cut(s) 236, 249, 273, 284, 692, 821, 1058, 1113
MspR9I CCNGG 3 cut(s) 236, 250, 1113
MvnI CGCG 1 cut(s) 201
MwoI GCNNNNNNNGC 3 cut(s) 299, 1226, 1235
NciI CCSGG 3 cut(s) 236, 250, 1113
NdeI CATATG 2 cut(s) 510, 826
NdeII GATC 9 cut(s) 67, 252, 269, 343, 688, 751, 961, 991, 1214
NlaIII CATG 6 cut(s) 149, 377, 389, 563, 770, 956
NlaIV GGNNCC 3 cut(s) 271, 606, 1042
NruI TCGCGA 1 cut(s) 201
NsiI ATGCAT 3 cut(s) 375, 561, 954
NspI RCATGY 2 cut(s) 377, 563
PaeI GCATGC 1 cut(s) 377
PagI TCATGA 1 cut(s) 385
PaqCI CACCTGC 1 cut(s) 488
PciSI GCTCTTC 2 cut(s) 30, 321
PcsI WCGNNNNNNNCGW 1 cut(s) 51
PfeI GAWTC 6 cut(s) 193, 389, 536, 817, 1128, 1264
PkrI GCNGC 3 cut(s) 418, 703, 1231
PleI GAGTC 1 cut(s) 861
PpsI GAGTC 1 cut(s) 861
PpuMI RGGWCCY 1 cut(s) 605
Psp1406I AACGTT 1 cut(s) 1223
Psp5II RGGWCCY 1 cut(s) 605
PspN4I GGNNCC 3 cut(s) 271, 606, 1042
PspPI GGNCC 2 cut(s) 605, 1040
PspPPI RGGWCCY 1 cut(s) 605
PsuI RGATCY 2 cut(s) 269, 751
RruI TCGCGA 1 cut(s) 201
RsaI GTAC 1 cut(s) 924
RsaNI GTAC 1 cut(s) 923
RseI CAYNNNNRTG 1 cut(s) 378
SapI GCTCTTC 2 cut(s) 30, 321
SaqAI TTAA 1 cut(s) 761
SatI GCNGC 3 cut(s) 417, 702, 1230
Sau3AI GATC 9 cut(s) 67, 252, 269, 343, 688, 751, 961, 991, 1214
Sau96I GGNCC 2 cut(s) 605, 1040
SchI GAGTC 1 cut(s) 862
ScrFI CCNGG 3 cut(s) 236, 250, 1113
SduI GDGCHC 2 cut(s) 190, 774
SfaNI GCATC 7 cut(s) 301, 546, 626, 713, 879, 920, 1267
SfcI CTRYAG 1 cut(s) 579
SinI GGWCC 2 cut(s) 605, 1040
SmiMI CAYNNNNRTG 1 cut(s) 378
SmlI CTYRAG 1 cut(s) 760
SmoI CTYRAG 1 cut(s) 760
SphI GCATGC 1 cut(s) 377
Sse9I AATT 6 cut(s) 7, 212, 422, 587, 833, 1007
SsiI CCGC 5 cut(s) 660, 798, 889, 938, 1076
SspI AATATT 1 cut(s) 549
SspMI CTAG 1 cut(s) 48
StyD4I CCNGG 3 cut(s) 234, 248, 1111
StyI CCWWGG 1 cut(s) 409
TaaI ACNGT 2 cut(s) 460, 985
TaiI ACGT 2 cut(s) 445, 1226
TaqI TCGA 3 cut(s) 168, 360, 1191
TasI AATT 6 cut(s) 7, 212, 422, 587, 833, 1007
TatI WGTACW 1 cut(s) 922
TfiI GAWTC 6 cut(s) 193, 389, 536, 817, 1128, 1264
Tru1I TTAA 1 cut(s) 761
Tru9I TTAA 1 cut(s) 761
TscAI CASTG 4 cut(s) 465, 640, 990, 1018
TseI GCWGC 3 cut(s) 416, 701, 1229
TspDTI ATGAA 7 cut(s) 162, 225, 497, 554, 803, 969, 1160
TspGWI ACGGA 2 cut(s) 43, 287
TspRI CASTG 4 cut(s) 465, 640, 990, 1018
Vha464I CTTAAG 1 cut(s) 760
VpaK11BI GGWCC 2 cut(s) 605, 1040
XapI RAATTY 2 cut(s) 833, 1007
XceI RCATGY 2 cut(s) 377, 563
XspI CTAG 1 cut(s) 48
Zsp2I ATGCAT 3 cut(s) 375, 561, 954
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.