pycom01g23790

Iaa-amino acid hydrolase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr1
OG0010280
Physical Location & Seq
Reverse (-)
21001175 .. 21002218
1044 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom01g23790.1

Sequence Viewer

Length: 627 bp
ATGCTGCAGAACCCCACAATACTGCTGATCCATCGCATACTTGCTGCCTCATTTGCAATTTTGGCGTTGCAACAGCTCATCTCAAGAGAAACTGATCCCCTCCATAGCCAAGTCCTATCTGTTACTTATGTTAGAGGTGGGACTGCATCGAATGTAATTCCATCGTATGTTGTATTTGGGGGCACTTTGAGGAGTCTTACAACTGAAGGCTTGTACTTACTCAGGAGGCGATTGAAAGAGGTTATTGAAGGACAGGCAGCCGTGCATAGATGTAACGCATATGTTGACACGAAAGATGAAGAATACCCACCATTGCCTGCTGTTGTCAACGATGAAAGCTTGCATATGCAGGCACAGAGGGTTGGAAAACTTTTGCTTGGTCCCGAGAATGTGAAGTTGTGCGAGAAAGTGATGGCAGGTGAAGACTTTGCCTTCTATCAGCAGTTTATTCCTGGAATCATGCTTAGCATTGGAATTGGAAATGAGGAAGTGGGTTCAGTTCACTCGCCGCACTCGCCTTACTTCTTTCTTGATGAGGATGTGCTTCCGATTGGGGCAGCATTACACGCTGCCTTGGCGGAGAGTTATTTGGACGAGCACCAACATTCTGTACACGCTGAAATTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009850 GO:0009987 GO:0010178 GO:0010817 GO:0016787 GO:0016810 GO:0042445 GO:0044237 GO:0065007 GO:0065008
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
M20_dimer Peptidase_M20

Protein Analysis

209

Amino Acids

22.94

Weight (kDa)

5.38

Isoelectric Point (pI)

43.77

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Peptidase_M20 PF01546 99 - 194 1.8e-09 Peptidase family M20/M25/M40
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 407
Acc36I ACCTGC 1 cut(s) 407
AciI CCGC 2 cut(s) 509, 578
AclWI GGATC 2 cut(s) 22, 89
AcsI RAATTY 1 cut(s) 621
AcuI CTGAAG 1 cut(s) 225
AfaI GTAC 2 cut(s) 215, 612
AgsI TTSAA 2 cut(s) 235, 248
AjnI CCWGG 1 cut(s) 451
AluBI AGCT 2 cut(s) 76, 339
AluI AGCT 2 cut(s) 76, 339
Alw21I GWGCWC 1 cut(s) 600
AlwI GGATC 2 cut(s) 22, 89
Ama87I CYCGRG 1 cut(s) 383
ApeKI GCWGC 5 cut(s) 4, 44, 257, 557, 569
ApoI RAATTY 1 cut(s) 621
AspS9I GGNCC 1 cut(s) 380
AsuHPI GGTGA 1 cut(s) 431
AvaI CYCGRG 1 cut(s) 383
AvaII GGWCC 1 cut(s) 380
BaeGI GKGCMC 1 cut(s) 185
BbsI GAAGAC 1 cut(s) 429
Bbv12I GWGCWC 1 cut(s) 600
BbvI GCAGC 4 cut(s) 31, 269, 556, 569
BccI CCATC 3 cut(s) 39, 169, 406
BceAI ACGGC 1 cut(s) 245
BciT130I CCWGG 1 cut(s) 453
BfmI CTRYAG 1 cut(s) 5
BfuAI ACCTGC 1 cut(s) 407
BisI GCNGC 6 cut(s) 5, 45, 258, 509, 558, 570
BlpI GCTNAGC 1 cut(s) 464
BlsI GCNGC 6 cut(s) 6, 46, 259, 510, 559, 571
Bme1390I CCNGG 1 cut(s) 453
Bme18I GGWCC 1 cut(s) 380
BmeT110I CYCGRG 1 cut(s) 383
BmgT120I GGNCC 1 cut(s) 380
BmiI GGNNCC 1 cut(s) 382
BmrFI CCNGG 1 cut(s) 453
BmsI GCATC 1 cut(s) 155
BpiI GAAGAC 1 cut(s) 429
Bpu1102I GCTNAGC 1 cut(s) 464
BpuEI CTTGAG 1 cut(s) 67
BsaJI CCNNGG 1 cut(s) 573
Bse3DI GCAATG 1 cut(s) 311
BseBI CCWGG 1 cut(s) 453
BseDI CCNNGG 1 cut(s) 573
BseGI GGATG 1 cut(s) 544
BseMI GCAATG 1 cut(s) 311
BseMII CTCAG 1 cut(s) 235
BseRI GAGGAG 1 cut(s) 205
BseSI GKGCMC 1 cut(s) 185
BseXI GCAGC 4 cut(s) 31, 269, 556, 569
BsiHKAI GWGCWC 1 cut(s) 600
BsiHKCI CYCGRG 1 cut(s) 383
BslFI GGGAC 2 cut(s) 154, 366
BsmFI GGGAC 2 cut(s) 154, 366
BsoBI CYCGRG 1 cut(s) 383
Bsp1286I GDGCHC 2 cut(s) 185, 600
Bsp1407I TGTACA 1 cut(s) 610
Bsp143I GATC 2 cut(s) 27, 94
Bsp1720I GCTNAGC 1 cut(s) 464
BspACI CCGC 2 cut(s) 509, 578
BspCNI CTCAG 1 cut(s) 234
BspLI GGNNCC 1 cut(s) 382
BspMAI CTGCAG 1 cut(s) 9
BspMI ACCTGC 1 cut(s) 407
BspPI GGATC 2 cut(s) 22, 89
BsrDI GCAATG 1 cut(s) 311
BsrGI TGTACA 1 cut(s) 610
BssECI CCNNGG 1 cut(s) 573
BssMI GATC 2 cut(s) 27, 94
BssT1I CCWWGG 1 cut(s) 573
Bst2UI CCWGG 1 cut(s) 453
BstAUI TGTACA 1 cut(s) 610
BstC8I GCNNGC 3 cut(s) 318, 341, 351
BstDEI CTNAG 2 cut(s) 221, 464
BstF5I GGATG 1 cut(s) 544
BstKTI GATC 2 cut(s) 30, 97
BstMBI GATC 2 cut(s) 27, 94
BstMWI GCNNNNNNNGC 5 cut(s) 53, 62, 514, 566, 575
BstNI CCWGG 1 cut(s) 453
BstSCI CCNGG 1 cut(s) 451
BstSFI CTRYAG 1 cut(s) 5
BstSLI GKGCMC 1 cut(s) 185
BstV1I GCAGC 4 cut(s) 31, 269, 556, 569
BstV2I GAAGAC 1 cut(s) 429
BtgZI GCGATG 1 cut(s) 17
BtsCI GGATG 1 cut(s) 544
BveI ACCTGC 1 cut(s) 407
Cac8I GCNNGC 3 cut(s) 318, 341, 351
Cfr13I GGNCC 1 cut(s) 380
Csp6I GTAC 2 cut(s) 214, 611
CviAII CATG 1 cut(s) 460
CviJI RGCY 5 cut(s) 76, 108, 210, 260, 339
CviKI_1 RGCY 5 cut(s) 76, 108, 210, 260, 339
CviQI GTAC 2 cut(s) 214, 611
DdeI CTNAG 2 cut(s) 221, 464
DpnI GATC 2 cut(s) 29, 96
DpnII GATC 2 cut(s) 27, 94
EciI GGCGGA 1 cut(s) 593
Eco130I CCWWGG 1 cut(s) 573
Eco47I GGWCC 1 cut(s) 380
Eco57I CTGAAG 1 cut(s) 225
Eco88I CYCGRG 1 cut(s) 383
EcoRII CCWGG 1 cut(s) 451
EcoT14I CCWWGG 1 cut(s) 573
ErhI CCWWGG 1 cut(s) 573
FaeI CATG 1 cut(s) 463
FaqI GGGAC 2 cut(s) 154, 366
FatI CATG 1 cut(s) 459
FauNDI CATATG 2 cut(s) 280, 345
Fnu4HI GCNGC 6 cut(s) 5, 45, 258, 509, 558, 570
FokI GGATG 1 cut(s) 551
Fsp4HI GCNGC 6 cut(s) 5, 45, 258, 509, 558, 570
GluI GCNGC 6 cut(s) 5, 45, 258, 509, 558, 570
Hin1II CATG 1 cut(s) 463
HincII GTYRAC 2 cut(s) 286, 328
HindII GTYRAC 2 cut(s) 286, 328
HindIII AAGCTT 1 cut(s) 337
HinfI GANTC 2 cut(s) 193, 456
HphI GGTGA 1 cut(s) 431
Hpy166II GTNNAC 4 cut(s) 286, 328, 502, 613
Hpy188I TCNGA 1 cut(s) 549
Hpy188III TCNNGA 4 cut(s) 84, 223, 383, 530
Hpy8I GTNNAC 4 cut(s) 286, 328, 502, 613
HpyAV CCTTC 3 cut(s) 200, 242, 442
HpyCH4V TGCA 7 cut(s) 7, 56, 70, 146, 265, 343, 349
HpyF10VI GCNNNNNNNGC 5 cut(s) 53, 62, 514, 566, 575
HpyF3I CTNAG 2 cut(s) 221, 464
Hsp92II CATG 1 cut(s) 463
Kzo9I GATC 2 cut(s) 27, 94
LpnPI CCDG 7 cut(s) 208, 239, 330, 335, 402, 438, 465
Lsp1109I GCAGC 4 cut(s) 31, 269, 556, 569
LweI GCATC 1 cut(s) 155
MaeIII GTNAC 2 cut(s) 121, 272
MalI GATC 2 cut(s) 29, 96
MboI GATC 2 cut(s) 27, 94
MboII GAAGA 2 cut(s) 311, 434
MhlI GDGCHC 2 cut(s) 185, 600
MluCI AATT 4 cut(s) 57, 156, 474, 621
MlyI GAGTC 1 cut(s) 202
MmeI TCCRAC 1 cut(s) 343
MnlI CCTC 9 cut(s) 58, 110, 128, 183, 219, 232, 351, 478, 529
MseI TTAA 1 cut(s) 625
MspR9I CCNGG 1 cut(s) 453
MvaI CCWGG 1 cut(s) 453
MwoI GCNNNNNNNGC 5 cut(s) 53, 62, 514, 566, 575
NdeI CATATG 2 cut(s) 280, 345
NdeII GATC 2 cut(s) 27, 94
NlaIII CATG 1 cut(s) 463
NlaIV GGNNCC 1 cut(s) 382
PaqCI CACCTGC 1 cut(s) 407
PfeI GAWTC 1 cut(s) 456
PfoI TCCNGGA 1 cut(s) 451
PkrI GCNGC 6 cut(s) 6, 46, 259, 510, 559, 571
PleI GAGTC 1 cut(s) 201
PpsI GAGTC 1 cut(s) 201
Psp6I CCWGG 1 cut(s) 451
PspGI CCWGG 1 cut(s) 451
PspN4I GGNNCC 1 cut(s) 382
PspPI GGNCC 1 cut(s) 380
PstI CTGCAG 1 cut(s) 9
RsaI GTAC 2 cut(s) 215, 612
RsaNI GTAC 2 cut(s) 214, 611
SaqAI TTAA 1 cut(s) 625
SatI GCNGC 6 cut(s) 5, 45, 258, 509, 558, 570
Sau3AI GATC 2 cut(s) 27, 94
Sau96I GGNCC 1 cut(s) 380
SchI GAGTC 1 cut(s) 202
ScrFI CCNGG 1 cut(s) 453
SduI GDGCHC 2 cut(s) 185, 600
SetI ASST 5 cut(s) 78, 139, 243, 341, 421
SfaNI GCATC 1 cut(s) 155
SfcI CTRYAG 1 cut(s) 5
SinI GGWCC 1 cut(s) 380
SmlI CTYRAG 1 cut(s) 82
SmoI CTYRAG 1 cut(s) 82
Sse9I AATT 4 cut(s) 57, 156, 474, 621
SsiI CCGC 2 cut(s) 509, 578
StyD4I CCNGG 1 cut(s) 451
StyI CCWWGG 1 cut(s) 573
TaqI TCGA 1 cut(s) 149
TasI AATT 4 cut(s) 57, 156, 474, 621
TatI WGTACW 2 cut(s) 213, 610
TauI GCSGC 1 cut(s) 511
TfiI GAWTC 1 cut(s) 456
Tru1I TTAA 1 cut(s) 625
Tru9I TTAA 1 cut(s) 625
TseI GCWGC 5 cut(s) 4, 44, 257, 557, 569
TspDTI ATGAA 2 cut(s) 312, 348
VpaK11BI GGWCC 1 cut(s) 380
XapI RAATTY 1 cut(s) 621
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.