Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr01

Orthogroup ID

OG0010280

Physical Location & Seq

Genomic Coordinates Reverse (-)

31898475 .. 31901351

Gene Structure 2877 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

MD01G1229800.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1488 bp

ATGACCGTGTACATTTTTAACTTCCTCCTACGACCGTTTGTATTTGTTTGGATACCGCGCGCCACACTTTACAAGTTAGTATATAAATTGCTGGTTGTACCCGTCCTTCCCTTATCGTCAACCCCTTCTGGTACTGCAACTGTTGACTTGCATCTCCGCAATTCCGTCAAACAAACAGAGATGCGTTCTTGTTCGATTGCCAATTTTCTCTTCGTTTTAGTACTCTTACTCACTTCTCTTTCTCTTGGTATCCCCCATGGCGCTCCTGATCAAACCCGCTACGCAAAAGAGCTTCTGAGCGCAGCCCAGCAGGATAAAAACTGGTTAATCTCCATAAGAAGGCAAATCCATGAAAACCCAGAACTCAAATTCCAAGAGCACAACACCAGCGCTCTTCTGCGCAGAGAACTGGATCGGCTCGGCATCCCTTACAACTACCCAATTGCCCAAACCGGAATTGTTGCAGAAATCGGGTCCGGTTCGAGCCCCGTTGTTGCTCTTCGAGCCGACATGGACGCCCTGCCGTTGCAAGAGCTTGTGGAGTGGGAGCACAAGAGTAAGGTTGATGGGAAAATGCACGGCTGTGGACATGATGCTCACACCACCATGCTTCTTGGCGCTGCTAAGTTGCTTAATCAACGAAAAGATAAACTTAAGGGAACTGTGAGACTTATTTTTCAACCTGGTGAGGAGGGAGGTGCTGGTGCCTCCAAAATGATAAAAGGAGGTGCTCTTGGTGAAGCTGAAGCGATATTTGGGATGCACGTTGATTATACAATACCCACAGGAACTGTAGCATCGATTGCAGGACCGCTCTTAGCTGCTGTTTCCATGTTTGAAGCCAAAATAGTCGGGATAGGCGGGCATGCTGCAGAACCCCACAATACTGCTGATCCAGTACTTGCTGCCTCATTTGCAATTTTGGCGTTGCAACAGCTCATCTCAAGAGAAACTGATCCCCTCCATAGCCAAGTCCTATCTGTTACTTATGTTAGAGGTGGGACTGCATCGAATGTAATTCCATCGTATGTTGTATTTGGGGGCACTTTGAGGAGTCTTACAACTGAAGGCTTGTACTTACTCAGGAGGCGATTGAAAGAGGTTATTGAAGGACAGGCAGCCGTGCATAGATGTAACGCATATGTTGACACGAAAGATGAAGAATACCCACCATTGCCTGCTGTTGTCAACGATGAAAGCTTGCATATGCAGGCACAGAGGGTTGGAAAACTTTTGCTTGGTCCCGAGAATGTGAAGTTGTGCAAGAAAGTGATGGCAGGTGAAGACTTTGCCTTCTATCAGCAGTTTATTCCTGGAATCATGCTTAGCATTGGAATTGGAAATGAGGAAGTGGGTTCAGTTCACTCGCCGCACTCGCCTTACTTCTTTCTTGATGAGGATGTGCTTCCGATTGGGGCAGCATTACACGCTGCCTTGGCGGAGAGTTATTTGGACGAGCACCAACATTCTGTACACGGTGAAATTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0003674 GO:0003824 GO:0008150 GO:0008152 GO:0009850 GO:0009987 GO:0010178 GO:0010817 GO:0016787 GO:0016810 GO:0042445 GO:0044237 GO:0065007 GO:0065008

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

M20_dimer Peptidase_M20

Protein Analysis

496

Amino Acids

53.98

Weight (kDa)

6.29

Isoelectric Point (pI)

34.93

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Peptidase_M20	PF01546	166 - 481	6.6e-43	Peptidase family M20/M25/M40
M20_dimer	PF07687	279 - 370	6.6e-08	Peptidase dimerisation domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010280)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54140
fragaria_vesca	FvH4_7g33061
malus_domestica	MD01G1229800.v1.1
prunus_persica	Prupe.2G320500_v2.0.a1 Prupe.2G320500_v2.0.a1 Prupe.2G320500_v2.0.a1 Prupe.2G320500_v2.0.a1
pyrus_communis	pycom01g23790
rosa_chinensis	RchiOBHm_Chr1g0383081
rosa_laevigata	RLG00000026134
rosa_multiflora	Rmu_sc0000634.1_g000007 Rmu_sc0008563.1_g000015 Rmu_sc0033623.1_g000003
rosa_roxburghii	Rroxscaffold_4G00277630
rosa_rugosa	Rorug01G0438200 Rorug01G0438300
rosa_samantha	Rh1AG463900 Rh1BG421100 Rh1CG434600 Rh1DG453700
rosa_wichuraiana	Rw1G040830

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	1268
Acc16I	TGCGCA	1 cut(s)	401
Acc36I	ACCTGC	1 cut(s)	1268
AccB1I	GGYRCC	1 cut(s)	704
AccBSI	CCGCTC	1 cut(s)	814
AccII	CGCG	2 cut(s)	58, 60
AciI	CCGC	7 cut(s)	56, 157, 277, 812, 861, 1370, 1439
AclWI	GGATC	3 cut(s)	420, 887, 950
AcsI	RAATTY	2 cut(s)	368, 1482
AcuI	CTGAAG	2 cut(s)	765, 1086
AcyI	GRCGYC	1 cut(s)	516
AfaI	GTAC	7 cut(s)	11, 99, 133, 222, 900, 1076, 1473
AfeI	AGCGCT	1 cut(s)	391
AfiI	CCNNNNNNNGG	1 cut(s)	339
AflII	CTTAAG	1 cut(s)	653
AgsI	TTSAA	4 cut(s)	680, 839, 1096, 1109
AjnI	CCWGG	2 cut(s)	682, 1312
AjuI	GAANNNNNNNTTGG	4 cut(s)	194, 226, 738, 770
AleI	CACNNNNGTG	1 cut(s)	582
AloI	GAACNNNNNNTCC	2 cut(s)	354, 386
AluBI	AGCT	6 cut(s)	292, 535, 743, 821, 937, 1200
AluI	AGCT	6 cut(s)	292, 535, 743, 821, 937, 1200
Alw21I	GWGCWC	4 cut(s)	381, 552, 733, 1461
Alw26I	GTCTC	1 cut(s)	661
AlwI	GGATC	3 cut(s)	420, 887, 950
AlwNI	CAGNNNCTG	1 cut(s)	791
Ama87I	CYCGRG	1 cut(s)	1244
Aor51HI	AGCGCT	1 cut(s)	391
ApeKI	GCWGC	8 cut(s)	302, 620, 821, 869, 905, 1118, 1418, 1430
ApoI	RAATTY	2 cut(s)	368, 1482
AspLEI	GCGC	7 cut(s)	60, 62, 263, 302, 392, 402, 620
AspS9I	GGNCC	3 cut(s)	474, 809, 1241
AsuHPI	GGTGA	3 cut(s)	698, 749, 1292
AvaI	CYCGRG	1 cut(s)	1244
AvaII	GGWCC	3 cut(s)	474, 809, 1241
BaeGI	GKGCMC	1 cut(s)	1046
BanI	GGYRCC	1 cut(s)	704
BanII	GRGCYC	1 cut(s)	488
BarI	GAAGNNNNNNTAC	2 cut(s)	90, 122
BbsI	GAAGAC	1 cut(s)	1290
Bbv12I	GWGCWC	4 cut(s)	381, 552, 733, 1461
BbvI	GCAGC	8 cut(s)	314, 607, 808, 856, 892, 1130, 1417, 1430
BccI	CCATC	3 cut(s)	560, 1030, 1267
BceAI	ACGGC	3 cut(s)	508, 595, 1106
BciT130I	CCWGG	2 cut(s)	684, 1314
BciVI	GTATCC	2 cut(s)	45, 260
BclI	TGATCA	1 cut(s)	268
BcoDI	GTCTC	1 cut(s)	661
BfmI	CTRYAG	2 cut(s)	792, 870
BfoI	RGCGCY	3 cut(s)	264, 393, 621
BfrI	CTTAAG	1 cut(s)	653
BfuAI	ACCTGC	1 cut(s)	1268
BfuI	GTATCC	2 cut(s)	45, 260
BisI	GCNGC	9 cut(s)	303, 621, 822, 870, 906, 1119, 1370, 1419, 1431
BlpI	GCTNAGC	1 cut(s)	1325
BlsI	GCNGC	9 cut(s)	304, 622, 823, 871, 907, 1120, 1371, 1420, 1432
BmcAI	AGTACT	2 cut(s)	222, 900
Bme1390I	CCNGG	2 cut(s)	684, 1314
Bme18I	GGWCC	3 cut(s)	474, 809, 1241
BmeT110I	CYCGRG	1 cut(s)	1244
BmgT120I	GGNCC	3 cut(s)	474, 809, 1241
BmiI	GGNNCC	3 cut(s)	475, 706, 1243
BmrFI	CCNGG	2 cut(s)	684, 1314
BmsI	GCATC	7 cut(s)	160, 171, 432, 583, 750, 806, 1016
BpiI	GAAGAC	1 cut(s)	1290
Bpu1102I	GCTNAGC	1 cut(s)	1325
BpuEI	CTTGAG	1 cut(s)	928
Bsa29I	ATCGAT	1 cut(s)	800
BsaHI	GRCGYC	1 cut(s)	516
BsaJI	CCNNGG	2 cut(s)	256, 1434
BsaWI	WCCGGW	2 cut(s)	452, 476
Bsc4I	CCNNNNNNNGG	1 cut(s)	339
Bse1I	ACTGG	3 cut(s)	326, 414, 896
Bse3DI	GCAATG	1 cut(s)	1172
BseBI	CCWGG	2 cut(s)	684, 1314
BseCI	ATCGAT	1 cut(s)	800
BseDI	CCNNGG	2 cut(s)	256, 1434
BseGI	GGATG	3 cut(s)	423, 765, 1405
BseLI	CCNNNNNNNGG	1 cut(s)	339
BseMI	GCAATG	1 cut(s)	1172
BseMII	CTCAG	2 cut(s)	287, 1096
BseNI	ACTGG	3 cut(s)	326, 414, 896
BsePI	GCGCGC	1 cut(s)	58
BseRI	GAGGAG	2 cut(s)	704, 1066
BseSI	GKGCMC	1 cut(s)	1046
BseXI	GCAGC	8 cut(s)	314, 607, 808, 856, 892, 1130, 1417, 1430
BseYI	CCCAGC	1 cut(s)	306
Bsh1236I	CGCG	2 cut(s)	58, 60
Bsh1285I	CGRYCG	1 cut(s)	35
BshNI	GGYRCC	1 cut(s)	704
BshVI	ATCGAT	1 cut(s)	800
BsiEI	CGRYCG	1 cut(s)	35
BsiHKAI	GWGCWC	4 cut(s)	381, 552, 733, 1461
BsiHKCI	CYCGRG	1 cut(s)	1244
BsiSI	CCGG	2 cut(s)	453, 477
BslFI	GGGAC	2 cut(s)	1015, 1227
BslI	CCNNNNNNNGG	1 cut(s)	339
BsmAI	GTCTC	1 cut(s)	661
BsmFI	GGGAC	2 cut(s)	1015, 1227
BsoBI	CYCGRG	1 cut(s)	1244
Bsp1286I	GDGCHC	6 cut(s)	381, 488, 552, 733, 1046, 1461
Bsp1407I	TGTACA	2 cut(s)	9, 1471
Bsp143I	GATC	4 cut(s)	268, 412, 892, 955
Bsp1720I	GCTNAGC	1 cut(s)	1325
Bsp19I	CCATGG	1 cut(s)	256
BspACI	CCGC	7 cut(s)	56, 157, 277, 812, 861, 1370, 1439
BspCNI	CTCAG	2 cut(s)	288, 1095
BspDI	ATCGAT	1 cut(s)	800
BspFNI	CGCG	2 cut(s)	58, 60
BspLI	GGNNCC	3 cut(s)	475, 706, 1243
BspMAI	CTGCAG	1 cut(s)	874
BspMI	ACCTGC	1 cut(s)	1268
BspPI	GGATC	3 cut(s)	420, 887, 950
BspQI	GCTCTTC	2 cut(s)	399, 504
BspT107I	GGYRCC	1 cut(s)	704
BspTI	CTTAAG	1 cut(s)	653
BsrBI	CCGCTC	1 cut(s)	814
BsrDI	GCAATG	1 cut(s)	1172
BsrGI	TGTACA	2 cut(s)	9, 1471
BsrI	ACTGG	3 cut(s)	326, 414, 896
BssECI	CCNNGG	2 cut(s)	256, 1434
BssHII	GCGCGC	1 cut(s)	58
BssMI	GATC	4 cut(s)	268, 412, 892, 955
BssNI	GRCGYC	1 cut(s)	516
BssT1I	CCWWGG	2 cut(s)	256, 1434
Bst2UI	CCWGG	2 cut(s)	684, 1314
Bst4CI	ACNGT	6 cut(s)	7, 36, 142, 664, 793, 1478
Bst6I	CTCTTC	3 cut(s)	215, 399, 504
BstACI	GRCGYC	1 cut(s)	516
BstAFI	CTTAAG	1 cut(s)	653
BstAPI	GCANNNNNTGC	1 cut(s)	803
BstAUI	TGTACA	2 cut(s)	9, 1471
BstC8I	GCNNGC	6 cut(s)	60, 863, 867, 1179, 1202, 1212
BstDEI	CTNAG	5 cut(s)	296, 624, 817, 1082, 1325
BstDSI	CCRYGG	1 cut(s)	256
BstF5I	GGATG	3 cut(s)	423, 765, 1405
BstFNI	CGCG	2 cut(s)	58, 60
BstH2I	RGCGCY	3 cut(s)	264, 393, 621
BstHHI	GCGC	7 cut(s)	60, 62, 263, 302, 392, 402, 620
BstKTI	GATC	4 cut(s)	271, 415, 895, 958
BstMAI	GTCTC	1 cut(s)	661
BstMBI	GATC	4 cut(s)	268, 412, 892, 955
BstMCI	CGRYCG	1 cut(s)	35
BstMWI	GCNNNNNNNGC	7 cut(s)	503, 803, 914, 923, 1375, 1427, 1436
BstNI	CCWGG	2 cut(s)	684, 1314
BstNSI	RCATGY	1 cut(s)	869
BstSCI	CCNGG	2 cut(s)	682, 1312
BstSFI	CTRYAG	2 cut(s)	792, 870
BstSLI	GKGCMC	1 cut(s)	1046
BstUI	CGCG	2 cut(s)	58, 60
BstV1I	GCAGC	8 cut(s)	314, 607, 808, 856, 892, 1130, 1417, 1430
BstV2I	GAAGAC	1 cut(s)	1290
Bsu15I	ATCGAT	1 cut(s)	800
BsuI	GTATCC	2 cut(s)	45, 260
BsuTUI	ATCGAT	1 cut(s)	800
BtgI	CCRYGG	1 cut(s)	256
BtsCI	GGATG	3 cut(s)	423, 765, 1405
BveI	ACCTGC	1 cut(s)	1268
Cac8I	GCNNGC	6 cut(s)	60, 863, 867, 1179, 1202, 1212
CaiI	CAGNNNCTG	1 cut(s)	791
CfoI	GCGC	7 cut(s)	60, 62, 263, 302, 392, 402, 620
Cfr13I	GGNCC	3 cut(s)	474, 809, 1241
ClaI	ATCGAT	1 cut(s)	800
CseI	GACGC	1 cut(s)	524
CsiI	ACCWGGT	1 cut(s)	682
Csp6I	GTAC	7 cut(s)	10, 98, 132, 221, 899, 1075, 1472
CviAII	CATG	8 cut(s)	257, 350, 511, 590, 607, 832, 866, 1321
CviQI	GTAC	7 cut(s)	10, 98, 132, 221, 899, 1075, 1472
DdeI	CTNAG	5 cut(s)	296, 624, 817, 1082, 1325
DpnI	GATC	4 cut(s)	270, 414, 894, 957
DpnII	GATC	4 cut(s)	268, 412, 892, 955
Eam1104I	CTCTTC	3 cut(s)	215, 399, 504
EarI	CTCTTC	3 cut(s)	215, 399, 504
EciI	GGCGGA	1 cut(s)	1454
Eco130I	CCWWGG	2 cut(s)	256, 1434
Eco24I	GRGCYC	1 cut(s)	488
Eco47I	GGWCC	3 cut(s)	474, 809, 1241
Eco47III	AGCGCT	1 cut(s)	391
Eco57I	CTGAAG	2 cut(s)	765, 1086
Eco88I	CYCGRG	1 cut(s)	1244
EcoRII	CCWGG	2 cut(s)	682, 1312
EcoT14I	CCWWGG	2 cut(s)	256, 1434
EcoT38I	GRGCYC	1 cut(s)	488
ErhI	CCWWGG	2 cut(s)	256, 1434
FaeI	CATG	8 cut(s)	260, 353, 514, 593, 610, 835, 869, 1324
FalI	AAGNNNNNCTT	2 cut(s)	636, 668
FaqI	GGGAC	2 cut(s)	1015, 1227
FatI	CATG	8 cut(s)	256, 349, 510, 589, 606, 831, 865, 1320
FauI	CCCGC	2 cut(s)	284, 854
FauNDI	CATATG	2 cut(s)	1141, 1206
FbaI	TGATCA	1 cut(s)	268
Fnu4HI	GCNGC	9 cut(s)	303, 621, 822, 870, 906, 1119, 1370, 1419, 1431
FokI	GGATG	3 cut(s)	410, 772, 1412
FriOI	GRGCYC	1 cut(s)	488
Fsp4HI	GCNGC	9 cut(s)	303, 621, 822, 870, 906, 1119, 1370, 1419, 1431
FspI	TGCGCA	1 cut(s)	401
GlaI	GCGC	7 cut(s)	59, 61, 262, 301, 391, 401, 619
GluI	GCNGC	9 cut(s)	303, 621, 822, 870, 906, 1119, 1370, 1419, 1431
GsaI	CCCAGC	1 cut(s)	310
HaeII	RGCGCY	3 cut(s)	264, 393, 621
HapII	CCGG	2 cut(s)	453, 477
HgaI	GACGC	1 cut(s)	524
HhaI	GCGC	7 cut(s)	60, 62, 263, 302, 392, 402, 620
Hin1I	GRCGYC	1 cut(s)	516
Hin1II	CATG	8 cut(s)	260, 353, 514, 593, 610, 835, 869, 1324
Hin6I	GCGC	7 cut(s)	58, 60, 261, 300, 390, 400, 618
HinP1I	GCGC	7 cut(s)	58, 60, 261, 300, 390, 400, 618
HincII	GTYRAC	4 cut(s)	120, 145, 1147, 1189
HindII	GTYRAC	4 cut(s)	120, 145, 1147, 1189
HindIII	AAGCTT	1 cut(s)	1198
HinfI	GANTC	2 cut(s)	1054, 1317
HpaII	CCGG	2 cut(s)	453, 477
HphI	GGTGA	3 cut(s)	698, 749, 1292
Hpy166II	GTNNAC	8 cut(s)	10, 120, 145, 587, 1147, 1189, 1363, 1474
Hpy188I	TCNGA	2 cut(s)	297, 1410
Hpy188III	TCNNGA	6 cut(s)	266, 853, 945, 1084, 1244, 1391
Hpy8I	GTNNAC	8 cut(s)	10, 120, 145, 587, 1147, 1189, 1363, 1474
HpyAV	CCTTC	6 cut(s)	116, 135, 333, 1061, 1103, 1303
HpyCH4III	ACNGT	6 cut(s)	7, 36, 142, 664, 793, 1478
HpyCH4IV	ACGT	1 cut(s)	765
HpyF10VI	GCNNNNNNNGC	7 cut(s)	503, 803, 914, 923, 1375, 1427, 1436
HpyF3I	CTNAG	5 cut(s)	296, 624, 817, 1082, 1325
HpySE526I	ACGT	1 cut(s)	765
Hsp92I	GRCGYC	1 cut(s)	516
Hsp92II	CATG	8 cut(s)	260, 353, 514, 593, 610, 835, 869, 1324
HspAI	GCGC	7 cut(s)	58, 60, 261, 300, 390, 400, 618
Ksp22I	TGATCA	1 cut(s)	268
Kzo9I	GATC	4 cut(s)	268, 412, 892, 955
LguI	GCTCTTC	2 cut(s)	399, 504
LmnI	GCTCC	2 cut(s)	268, 547
Lsp1109I	GCAGC	8 cut(s)	314, 607, 808, 856, 892, 1130, 1417, 1430
LweI	GCATC	7 cut(s)	160, 171, 432, 583, 750, 806, 1016
MabI	ACCWGGT	1 cut(s)	682
MaeII	ACGT	1 cut(s)	765
MaeIII	GTNAC	2 cut(s)	982, 1133
MalI	GATC	4 cut(s)	270, 414, 894, 957
MbiI	CCGCTC	1 cut(s)	814
MboI	GATC	4 cut(s)	268, 412, 892, 955
MboII	GAAGA	5 cut(s)	202, 386, 491, 1172, 1295
MfeI	CAATTG	1 cut(s)	441
MhlI	GDGCHC	6 cut(s)	381, 488, 552, 733, 1046, 1461
MlyI	GAGTC	1 cut(s)	1063
MmeI	TCCRAC	1 cut(s)	1204
MseI	TTAA	5 cut(s)	18, 326, 633, 654, 1486
MslI	CAYNNNNRTG	2 cut(s)	582, 605
MspCI	CTTAAG	1 cut(s)	653
MspI	CCGG	2 cut(s)	453, 477
MspR9I	CCNGG	2 cut(s)	684, 1314
MunI	CAATTG	1 cut(s)	441
MvaI	CCWGG	2 cut(s)	684, 1314
MvnI	CGCG	2 cut(s)	58, 60
MwoI	GCNNNNNNNGC	7 cut(s)	503, 803, 914, 923, 1375, 1427, 1436
NcoI	CCATGG	1 cut(s)	256
NdeI	CATATG	2 cut(s)	1141, 1206
NdeII	GATC	4 cut(s)	268, 412, 892, 955
NlaIII	CATG	8 cut(s)	260, 353, 514, 593, 610, 835, 869, 1324
NlaIV	GGNNCC	3 cut(s)	475, 706, 1243
NmeAIII	GCCGAG	1 cut(s)	399
NsbI	TGCGCA	1 cut(s)	401
NspI	RCATGY	1 cut(s)	869
OliI	CACNNNNGTG	1 cut(s)	582
PaeI	GCATGC	1 cut(s)	869
PaqCI	CACCTGC	1 cut(s)	1268
PauI	GCGCGC	1 cut(s)	58
PciSI	GCTCTTC	2 cut(s)	399, 504
PfeI	GAWTC	1 cut(s)	1317
PfoI	TCCNGGA	1 cut(s)	1312
PkrI	GCNGC	9 cut(s)	304, 622, 823, 871, 907, 1120, 1371, 1420, 1432
PleI	GAGTC	1 cut(s)	1062
PpsI	GAGTC	1 cut(s)	1062
Psp6I	CCWGG	2 cut(s)	682, 1312
PspFI	CCCAGC	1 cut(s)	306
PspGI	CCWGG	2 cut(s)	682, 1312
PspN4I	GGNNCC	3 cut(s)	475, 706, 1243
PspPI	GGNCC	3 cut(s)	474, 809, 1241
PstI	CTGCAG	1 cut(s)	874
PstNI	CAGNNNCTG	1 cut(s)	791
PteI	GCGCGC	1 cut(s)	58
RsaI	GTAC	7 cut(s)	11, 99, 133, 222, 900, 1076, 1473
RsaNI	GTAC	7 cut(s)	10, 98, 132, 221, 899, 1075, 1472
RseI	CAYNNNNRTG	2 cut(s)	582, 605
SapI	GCTCTTC	2 cut(s)	399, 504
SaqAI	TTAA	5 cut(s)	18, 326, 633, 654, 1486
SatI	GCNGC	9 cut(s)	303, 621, 822, 870, 906, 1119, 1370, 1419, 1431
Sau3AI	GATC	4 cut(s)	268, 412, 892, 955
Sau96I	GGNCC	3 cut(s)	474, 809, 1241
ScaI	AGTACT	2 cut(s)	222, 900
SchI	GAGTC	1 cut(s)	1063
ScrFI	CCNGG	2 cut(s)	684, 1314
SduI	GDGCHC	6 cut(s)	381, 488, 552, 733, 1046, 1461
SexAI	ACCWGGT	1 cut(s)	682
SfaNI	GCATC	7 cut(s)	160, 171, 432, 583, 750, 806, 1016
SfcI	CTRYAG	2 cut(s)	792, 870
SinI	GGWCC	3 cut(s)	474, 809, 1241
SmiMI	CAYNNNNRTG	2 cut(s)	582, 605
SmlI	CTYRAG	2 cut(s)	653, 943
SmoI	CTYRAG	2 cut(s)	653, 943
SphI	GCATGC	1 cut(s)	869
SsiI	CCGC	7 cut(s)	56, 157, 277, 812, 861, 1370, 1439
StyD4I	CCNGG	2 cut(s)	682, 1312
StyI	CCWWGG	2 cut(s)	256, 1434
TaaI	ACNGT	6 cut(s)	7, 36, 142, 664, 793, 1478
TaiI	ACGT	1 cut(s)	768
TaqI	TCGA	5 cut(s)	194, 482, 502, 800, 1010
TatI	WGTACW	5 cut(s)	9, 220, 898, 1074, 1471
TauI	GCSGC	1 cut(s)	1372
TfiI	GAWTC	1 cut(s)	1317
Tru1I	TTAA	5 cut(s)	18, 326, 633, 654, 1486
Tru9I	TTAA	5 cut(s)	18, 326, 633, 654, 1486
TseI	GCWGC	8 cut(s)	302, 620, 821, 869, 905, 1118, 1418, 1430
TspDTI	ATGAA	3 cut(s)	366, 1173, 1209
TspGWI	ACGGA	1 cut(s)	154
Vha464I	CTTAAG	1 cut(s)	653
VpaK11BI	GGWCC	3 cut(s)	474, 809, 1241
XapI	RAATTY	2 cut(s)	368, 1482
XceI	RCATGY	1 cut(s)	869
ZrmI	AGTACT	2 cut(s)	222, 900

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.