AT5G54660

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009516
Physical Location & Seq
Forward (+)
22203717 .. 22205152
1436 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G54660.1

Sequence Viewer

Length: 579 bp
ATGACGAGTAGTAGTGGAAGCCTCAAACTAGAGATCCACACTGATGACAAGACGCCGGGAAAGTGGAGCGTACCGCTCGGCGATGATGTTTTCCGAAGGTTCTTGAGCGGGGGAGGAGGCTCGGAGAAGGCTGTGTTCGGTGAGGGGTCTCTTTTTAGCCCGTTTTTGTTCGGGAAGTACTTTGATCCGTCAGACGCCTTTCCGTTGTGGGAATTTGAAGCTGAGGTTTTGCTGGCGAGCCTTAGGAGCTTAGGACAGTGTAGAGTCGATTGGTCTCAGACGGATCAAGCTTATGTCCTTAAATCCGATATCCCCGTGGTGGGGAAAAATAACGTGCAGGTGTACGTGGACATTAACGGAAGAGTGATGGAGATTAGTGGCCAATGGAACAGCAACAAGAAAGCGGCGACCAACAGTGACTGGAGAAGCGGCCGGTGGTGGGAACATGGCTATGTCCGCCGTCTCGAGCTTCCTAGCGATGCCGACGCCAAATACTCTGAAGCTTTCCTCTCTAACAACGACGATTACTCTTTCTTAGAGATCCGAATCCCTAAGATAAATTCTAAGAACAAGTTTTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

192

Amino Acids

21.66

Weight (kDa)

5.47

Isoelectric Point (pI)

38.03

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 90 - 187 8.6e-06 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 328
Acc36I ACCTGC 1 cut(s) 328
AccBSI CCGCTC 2 cut(s) 76, 108
AciI CCGC 5 cut(s) 74, 108, 404, 429, 457
AclWI GGATC 4 cut(s) 28, 179, 291, 535
AcoI YGGCCR 2 cut(s) 379, 430
AcsI RAATTY 2 cut(s) 212, 559
AcuI CTGAAG 1 cut(s) 519
AcyI GRCGYC 3 cut(s) 53, 195, 486
AfaI GTAC 3 cut(s) 72, 179, 344
AfiI CCNNNNNNNGG 4 cut(s) 319, 320, 321, 439
AgsI TTSAA 1 cut(s) 218
AluBI AGCT 5 cut(s) 221, 249, 290, 469, 503
AluI AGCT 5 cut(s) 221, 249, 290, 469, 503
Alw26I GTCTC 3 cut(s) 153, 279, 467
AlwI GGATC 4 cut(s) 28, 179, 291, 535
AlwNI CAGNNNCTG 1 cut(s) 420
Ama87I CYCGRG 1 cut(s) 464
AoxI GGCC 2 cut(s) 379, 430
ApoI RAATTY 2 cut(s) 212, 559
AsuC2I CCSGG 1 cut(s) 57
AsuHPI GGTGA 1 cut(s) 152
AvaI CYCGRG 1 cut(s) 464
AxyI CCTNAGG 1 cut(s) 242
BalI TGGCCA 1 cut(s) 381
BbvCI CCTCAGC 1 cut(s) 222
BccI CCATC 1 cut(s) 361
BceAI ACGGC 1 cut(s) 444
BcnI CCSGG 1 cut(s) 57
BcoDI GTCTC 3 cut(s) 153, 279, 467
BfaI CTAG 2 cut(s) 29, 474
BfuAI ACCTGC 1 cut(s) 328
BisI GCNGC 2 cut(s) 405, 430
BlsI GCNGC 2 cut(s) 406, 431
BmcAI AGTACT 1 cut(s) 179
Bme1390I CCNGG 1 cut(s) 57
BmeT110I CYCGRG 1 cut(s) 464
BmrFI CCNGG 1 cut(s) 57
BmsI GCATC 1 cut(s) 469
BpmI CTGGAG 1 cut(s) 442
Bpu10I CCTNAGC 2 cut(s) 222, 250
BpuEI CTTGAG 1 cut(s) 124
BpuMI CCSGG 1 cut(s) 57
BsaAI YACGTR 1 cut(s) 346
BsaBI GATNNNNATC 1 cut(s) 545
BsaHI GRCGYC 3 cut(s) 53, 195, 486
BsaI GGTCTC 2 cut(s) 153, 279
BsaJI CCNNGG 1 cut(s) 315
Bsc4I CCNNNNNNNGG 4 cut(s) 319, 320, 321, 439
Bse118I RCCGGY 1 cut(s) 432
Bse1I ACTGG 1 cut(s) 425
Bse21I CCTNAGG 1 cut(s) 242
Bse8I GATNNNNATC 1 cut(s) 545
BseDI CCNNGG 1 cut(s) 315
BseJI GATNNNNATC 1 cut(s) 545
BseLI CCNNNNNNNGG 4 cut(s) 319, 320, 321, 439
BseMII CTCAG 2 cut(s) 213, 290
BseNI ACTGG 1 cut(s) 425
BseRI GAGGAG 1 cut(s) 129
BseX3I CGGCCG 1 cut(s) 430
BsgI GTGCAG 1 cut(s) 356
Bsh1285I CGRYCG 1 cut(s) 433
BshFI GGCC 2 cut(s) 381, 432
BsiEI CGRYCG 1 cut(s) 433
BsiHKCI CYCGRG 1 cut(s) 464
BsiSI CCGG 2 cut(s) 56, 433
BslI CCNNNNNNNGG 4 cut(s) 319, 320, 321, 439
BsmAI GTCTC 3 cut(s) 153, 279, 467
BsmBI CGTCTC 1 cut(s) 467
BsnI GGCC 2 cut(s) 381, 432
Bso31I GGTCTC 2 cut(s) 153, 279
BsoBI CYCGRG 1 cut(s) 464
Bsp143I GATC 4 cut(s) 33, 184, 283, 540
BspACI CCGC 5 cut(s) 74, 108, 404, 429, 457
BspANI GGCC 2 cut(s) 381, 432
BspCNI CTCAG 2 cut(s) 214, 289
BspMI ACCTGC 1 cut(s) 328
BspPI GGATC 4 cut(s) 28, 179, 291, 535
BspTNI GGTCTC 2 cut(s) 153, 279
BsrBI CCGCTC 2 cut(s) 76, 108
BsrFI RCCGGY 1 cut(s) 432
BsrI ACTGG 1 cut(s) 425
BssAI RCCGGY 1 cut(s) 432
BssECI CCNNGG 1 cut(s) 315
BssMI GATC 4 cut(s) 33, 184, 283, 540
BssNI GRCGYC 3 cut(s) 53, 195, 486
Bst4CI ACNGT 2 cut(s) 258, 416
Bst6I CTCTTC 1 cut(s) 355
BstACI GRCGYC 3 cut(s) 53, 195, 486
BstBAI YACGTR 1 cut(s) 346
BstC8I GCNNGC 2 cut(s) 234, 238
BstDEI CTNAG 7 cut(s) 222, 242, 250, 276, 535, 552, 564
BstDSI CCRYGG 1 cut(s) 315
BstKTI GATC 4 cut(s) 36, 187, 286, 543
BstMAI GTCTC 3 cut(s) 153, 279, 467
BstMBI GATC 4 cut(s) 33, 184, 283, 540
BstMCI CGRYCG 1 cut(s) 433
BstMWI GCNNNNNNNGC 2 cut(s) 246, 456
BstSCI CCNGG 1 cut(s) 55
BstX2I RGATCY 2 cut(s) 33, 540
BstYI RGATCY 2 cut(s) 33, 540
BstZI CGGCCG 1 cut(s) 430
Bsu36I CCTNAGG 1 cut(s) 242
BsuRI GGCC 2 cut(s) 381, 432
BtgI CCRYGG 1 cut(s) 315
BtgZI GCGATG 2 cut(s) 96, 492
BtsIMutI CAGTG 3 cut(s) 39, 263, 421
BveI ACCTGC 1 cut(s) 328
Cac8I GCNNGC 2 cut(s) 234, 238
CaiI CAGNNNCTG 1 cut(s) 420
Cfr10I RCCGGY 1 cut(s) 432
CseI GACGC 3 cut(s) 61, 203, 494
Csp6I GTAC 3 cut(s) 71, 178, 343
CviAII CATG 1 cut(s) 446
CviQI GTAC 3 cut(s) 71, 178, 343
DdeI CTNAG 7 cut(s) 222, 242, 250, 276, 535, 552, 564
DpnI GATC 4 cut(s) 35, 186, 285, 542
DpnII GATC 4 cut(s) 33, 184, 283, 540
EaeI YGGCCR 2 cut(s) 379, 430
EagI CGGCCG 1 cut(s) 430
Eam1104I CTCTTC 1 cut(s) 355
EarI CTCTTC 1 cut(s) 355
EciI GGCGGA 1 cut(s) 446
EclXI CGGCCG 1 cut(s) 430
Eco31I GGTCTC 2 cut(s) 153, 279
Eco32I GATATC 1 cut(s) 310
Eco52I CGGCCG 1 cut(s) 430
Eco57I CTGAAG 1 cut(s) 519
Eco81I CCTNAGG 1 cut(s) 242
Eco88I CYCGRG 1 cut(s) 464
EcoRV GATATC 1 cut(s) 310
Esp3I CGTCTC 1 cut(s) 467
FaeI CATG 1 cut(s) 449
FaiI YATR 3 cut(s) 294, 447, 453
FatI CATG 1 cut(s) 445
FauI CCCGC 1 cut(s) 101
Fnu4HI GCNGC 2 cut(s) 405, 430
Fsp4HI GCNGC 2 cut(s) 405, 430
FspBI CTAG 2 cut(s) 29, 474
GluI GCNGC 2 cut(s) 405, 430
GsuI CTGGAG 1 cut(s) 442
HaeIII GGCC 2 cut(s) 381, 432
HapII CCGG 2 cut(s) 56, 433
HgaI GACGC 3 cut(s) 61, 203, 494
Hin1I GRCGYC 3 cut(s) 53, 195, 486
Hin1II CATG 1 cut(s) 449
HindIII AAGCTT 2 cut(s) 288, 501
HinfI GANTC 2 cut(s) 264, 546
HpaII CCGG 2 cut(s) 56, 433
HphI GGTGA 1 cut(s) 152
Hpy166II GTNNAC 2 cut(s) 343, 349
Hpy188I TCNGA 7 cut(s) 95, 124, 193, 279, 307, 499, 545
Hpy188III TCNNGA 3 cut(s) 103, 172, 464
Hpy8I GTNNAC 2 cut(s) 343, 349
Hpy99I CGWCG 2 cut(s) 488, 524
HpyAV CCTTC 2 cut(s) 90, 121
HpyCH4III ACNGT 2 cut(s) 258, 416
HpyCH4IV ACGT 2 cut(s) 333, 345
HpyCH4V TGCA 1 cut(s) 337
HpyF10VI GCNNNNNNNGC 2 cut(s) 246, 456
HpyF3I CTNAG 7 cut(s) 222, 242, 250, 276, 535, 552, 564
HpySE526I ACGT 2 cut(s) 333, 345
Hsp92I GRCGYC 3 cut(s) 53, 195, 486
Hsp92II CATG 1 cut(s) 449
Kzo9I GATC 4 cut(s) 33, 184, 283, 540
LmnI GCTCC 2 cut(s) 66, 246
LpnPI CCDG 5 cut(s) 69, 218, 323, 406, 446
LweI GCATC 1 cut(s) 469
MaeI CTAG 2 cut(s) 29, 474
MaeII ACGT 2 cut(s) 333, 345
MaeIII GTNAC 1 cut(s) 416
MalI GATC 4 cut(s) 35, 186, 285, 542
MbiI CCGCTC 2 cut(s) 76, 108
MboI GATC 4 cut(s) 33, 184, 283, 540
MboII GAAGA 1 cut(s) 372
MflI RGATCY 2 cut(s) 33, 540
MlsI TGGCCA 1 cut(s) 381
MluCI AATT 2 cut(s) 212, 559
MluNI TGGCCA 1 cut(s) 381
MlyI GAGTC 1 cut(s) 273
MnlI CCTC 6 cut(s) 32, 107, 110, 136, 217, 518
Mox20I TGGCCA 1 cut(s) 381
MscI TGGCCA 1 cut(s) 381
MseI TTAA 3 cut(s) 300, 354, 577
MslI CAYNNNNRTG 2 cut(s) 42, 450
Msp20I TGGCCA 1 cut(s) 381
MspI CCGG 2 cut(s) 56, 433
MspR9I CCNGG 1 cut(s) 57
MwoI GCNNNNNNNGC 2 cut(s) 246, 456
NciI CCSGG 1 cut(s) 57
NdeII GATC 4 cut(s) 33, 184, 283, 540
NlaIII CATG 1 cut(s) 449
NmeAIII GCCGAG 1 cut(s) 57
NmuCI GTSAC 1 cut(s) 416
PaeR7I CTCGAG 1 cut(s) 464
PaqCI CACCTGC 1 cut(s) 328
PfeI GAWTC 1 cut(s) 546
PkrI GCNGC 2 cut(s) 406, 431
PleI GAGTC 1 cut(s) 272
PpsI GAGTC 1 cut(s) 272
Ppu21I YACGTR 1 cut(s) 346
PstNI CAGNNNCTG 1 cut(s) 420
PsuI RGATCY 2 cut(s) 33, 540
RsaI GTAC 3 cut(s) 72, 179, 344
RsaNI GTAC 3 cut(s) 71, 178, 343
RseI CAYNNNNRTG 2 cut(s) 42, 450
SaqAI TTAA 3 cut(s) 300, 354, 577
SatI GCNGC 2 cut(s) 405, 430
Sau3AI GATC 4 cut(s) 33, 184, 283, 540
ScaI AGTACT 1 cut(s) 179
SchI GAGTC 1 cut(s) 273
ScrFI CCNGG 1 cut(s) 57
SfaNI GCATC 1 cut(s) 469
Sfr274I CTCGAG 1 cut(s) 464
SlaI CTCGAG 1 cut(s) 464
SmiMI CAYNNNNRTG 2 cut(s) 42, 450
SmlI CTYRAG 2 cut(s) 103, 464
SmoI CTYRAG 2 cut(s) 103, 464
Sse9I AATT 2 cut(s) 212, 559
SsiI CCGC 5 cut(s) 74, 108, 404, 429, 457
SspMI CTAG 2 cut(s) 29, 474
StyD4I CCNGG 1 cut(s) 55
TaaI ACNGT 2 cut(s) 258, 416
TaiI ACGT 2 cut(s) 336, 348
TaqI TCGA 2 cut(s) 267, 465
TasI AATT 2 cut(s) 212, 559
TatI WGTACW 1 cut(s) 177
TauI GCSGC 2 cut(s) 407, 432
TfiI GAWTC 1 cut(s) 546
Tru1I TTAA 3 cut(s) 300, 354, 577
Tru9I TTAA 3 cut(s) 300, 354, 577
TscAI CASTG 3 cut(s) 46, 263, 421
TseFI GTSAC 1 cut(s) 416
Tsp45I GTSAC 1 cut(s) 416
TspGWI ACGGA 4 cut(s) 177, 192, 296, 372
TspRI CASTG 3 cut(s) 46, 263, 421
XapI RAATTY 2 cut(s) 212, 559
XhoI CTCGAG 1 cut(s) 464
XspI CTAG 2 cut(s) 29, 474
ZrmI AGTACT 1 cut(s) 179
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.