Rh5CG287400

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5C
OG0009516
Physical Location & Seq
Forward (+)
31504018 .. 31505861
1844 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5CG287400.1

Sequence Viewer

Length: 606 bp
ATGACTACCACTACTCACAAACAGCTTGAAGTTCTTACAGATGATCAAACACCACAGAAATGGTGTGTTCTATTGAGAGAAGACGTGTTCAAAAAGTTCATGTCTCAAGGCAGTCCAATAGTGCACAAGGTGTTTGGTGTTGGATCATTTTTCAGTCCTCTTTTGTTTGGGAAATTCTTTGATCCATCTGATGCTTTTCCGCTGTGGGACTTTGAGTCAGATATCTTATTGTCTAGTCTAAGAAGCTCTGGCCAAAGCACTATTGATTGGTTTCAGACAGAGCAATATTATGTGCTCAAAGCAGAACTACCTGGAGAAGGGAAAAACAGTGTTCAAGTGTGTGCTGAAAATGCGAAGGTTTTGGAGATTAGTGGGCAGTGGAAGCAGCAGCAACAGCAAAAAGATAAGTCCAAGACAAACAGTACAAGAGATTGGAGAAGTGGGAATTGGTGGGAATCTGGGTATGTTAGGAGGCTTGAGCTCCCACAAGATGCAGATTGGAGAAAGATAGAGGCATATGTGACCAATGACCTGATTCTGGAAATCAAAATTTACAAGATCAACAACTCCTTGGATGCTGATAATAATCAGAACACTACTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

201

Amino Acids

23.24

Weight (kDa)

5.29

Isoelectric Point (pI)

46.42

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 89 - 189 6.2e-08 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 200
AclWI GGATC 2 cut(s) 151, 176
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 2 cut(s) 173, 549
AdeI CACNNNGTG 1 cut(s) 130
AfaI GTAC 1 cut(s) 424
AfiI CCNNNNNNNGG 2 cut(s) 317, 538
AflIII ACRYGT 1 cut(s) 84
AgsI TTSAA 3 cut(s) 29, 91, 335
AhdI GACNNNNNGTC 1 cut(s) 214
AjiI CACGTC 1 cut(s) 85
AjnI CCWGG 1 cut(s) 310
AjuI GAANNNNNNNTTGG 2 cut(s) 430, 462
AluBI AGCT 3 cut(s) 25, 246, 481
AluI AGCT 3 cut(s) 25, 246, 481
Alw21I GWGCWC 3 cut(s) 126, 297, 483
Alw26I GTCTC 1 cut(s) 108
Alw44I GTGCAC 1 cut(s) 122
AlwI GGATC 2 cut(s) 151, 176
AoxI GGCC 1 cut(s) 250
ApaLI GTGCAC 1 cut(s) 122
ApeKI GCWGC 2 cut(s) 385, 388
ApoI RAATTY 2 cut(s) 173, 549
BaeGI GKGCMC 1 cut(s) 126
BalI TGGCCA 1 cut(s) 252
BanII GRGCYC 1 cut(s) 483
BbsI GAAGAC 1 cut(s) 87
Bbv12I GWGCWC 3 cut(s) 126, 297, 483
BbvI GCAGC 2 cut(s) 397, 400
BccI CCATC 1 cut(s) 193
BciT130I CCWGG 1 cut(s) 312
BclI TGATCA 1 cut(s) 43
BcoDI GTCTC 1 cut(s) 108
BfaI CTAG 1 cut(s) 234
BisI GCNGC 2 cut(s) 386, 389
BlsI GCNGC 2 cut(s) 387, 390
Bme1390I CCNGG 1 cut(s) 312
BmeRI GACNNNNNGTC 1 cut(s) 214
BmgBI CACGTC 1 cut(s) 85
BmrFI CCNGG 1 cut(s) 312
BmsI GCATC 3 cut(s) 181, 481, 565
BpiI GAAGAC 1 cut(s) 87
BpmI CTGGAG 1 cut(s) 333
BpuEI CTTGAG 2 cut(s) 90, 497
BsaBI GATNNNNATC 1 cut(s) 585
BsaJI CCNNGG 1 cut(s) 570
Bsc4I CCNNNNNNNGG 2 cut(s) 317, 538
Bse8I GATNNNNATC 1 cut(s) 585
BseBI CCWGG 1 cut(s) 312
BseDI CCNNGG 1 cut(s) 570
BseGI GGATG 1 cut(s) 580
BseJI GATNNNNATC 1 cut(s) 585
BseLI CCNNNNNNNGG 2 cut(s) 317, 538
BseSI GKGCMC 1 cut(s) 126
BseXI GCAGC 2 cut(s) 397, 400
BshFI GGCC 1 cut(s) 252
BsiHKAI GWGCWC 3 cut(s) 126, 297, 483
BslFI GGGAC 1 cut(s) 221
BslI CCNNNNNNNGG 2 cut(s) 317, 538
BsmAI GTCTC 1 cut(s) 108
BsmFI GGGAC 1 cut(s) 221
BsnI GGCC 1 cut(s) 252
Bsp1286I GDGCHC 3 cut(s) 126, 297, 483
Bsp143I GATC 4 cut(s) 43, 143, 181, 558
BspACI CCGC 1 cut(s) 200
BspANI GGCC 1 cut(s) 252
BspPI GGATC 2 cut(s) 151, 176
BssECI CCNNGG 1 cut(s) 570
BssMI GATC 4 cut(s) 43, 143, 181, 558
BssT1I CCWWGG 1 cut(s) 570
Bst2UI CCWGG 1 cut(s) 312
Bst4CI ACNGT 2 cut(s) 329, 422
BstDEI CTNAG 1 cut(s) 239
BstENI CCTNNNNNAGG 1 cut(s) 315
BstF5I GGATG 1 cut(s) 580
BstKTI GATC 4 cut(s) 46, 146, 184, 561
BstMAI GTCTC 1 cut(s) 108
BstMBI GATC 4 cut(s) 43, 143, 181, 558
BstMWI GCNNNNNNNGC 3 cut(s) 350, 382, 394
BstNI CCWGG 1 cut(s) 312
BstSCI CCNGG 1 cut(s) 310
BstSLI GKGCMC 1 cut(s) 126
BstV1I GCAGC 2 cut(s) 397, 400
BstV2I GAAGAC 1 cut(s) 87
BstXI CCANNNNNNTGG 1 cut(s) 60
BsuRI GGCC 1 cut(s) 252
BtrI CACGTC 1 cut(s) 85
BtsCI GGATG 1 cut(s) 580
BtsI GCAGTG 1 cut(s) 383
BtsIMutI CAGTG 2 cut(s) 334, 383
Csp6I GTAC 1 cut(s) 423
CviAII CATG 1 cut(s) 100
CviJI RGCY 5 cut(s) 25, 246, 252, 475, 481
CviKI_1 RGCY 5 cut(s) 25, 246, 252, 475, 481
CviQI GTAC 1 cut(s) 423
DdeI CTNAG 1 cut(s) 239
DpnI GATC 4 cut(s) 45, 145, 183, 560
DpnII GATC 4 cut(s) 43, 143, 181, 558
DraIII CACNNNGTG 1 cut(s) 130
DriI GACNNNNNGTC 1 cut(s) 214
EaeI YGGCCR 1 cut(s) 250
Eam1105I GACNNNNNGTC 1 cut(s) 214
Ecl136II GAGCTC 1 cut(s) 481
Eco130I CCWWGG 1 cut(s) 570
Eco24I GRGCYC 1 cut(s) 483
Eco32I GATATC 1 cut(s) 223
Eco53kI GAGCTC 1 cut(s) 481
EcoICRI GAGCTC 1 cut(s) 481
EcoNI CCTNNNNNAGG 1 cut(s) 315
EcoRII CCWGG 1 cut(s) 310
EcoRV GATATC 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 570
EcoT38I GRGCYC 1 cut(s) 483
ErhI CCWWGG 1 cut(s) 570
FaeI CATG 1 cut(s) 103
FaiI YATR 6 cut(s) 101, 291, 465, 517, 519, 604
FaqI GGGAC 1 cut(s) 221
FatI CATG 1 cut(s) 99
FauNDI CATATG 1 cut(s) 517
FbaI TGATCA 1 cut(s) 43
Fnu4HI GCNGC 2 cut(s) 386, 389
FokI GGATG 1 cut(s) 587
FriOI GRGCYC 1 cut(s) 483
Fsp4HI GCNGC 2 cut(s) 386, 389
FspBI CTAG 1 cut(s) 234
GluI GCNGC 2 cut(s) 386, 389
GsuI CTGGAG 1 cut(s) 333
HaeIII GGCC 1 cut(s) 252
Hin1II CATG 1 cut(s) 103
HinfI GANTC 3 cut(s) 215, 455, 535
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 4 cut(s) 190, 220, 276, 591
Hpy188III TCNNGA 1 cut(s) 539
Hpy8I GTNNAC 1 cut(s) 124
HpyAV CCTTC 2 cut(s) 311, 349
HpyCH4III ACNGT 2 cut(s) 329, 422
HpyCH4IV ACGT 1 cut(s) 84
HpyCH4V TGCA 3 cut(s) 124, 494, 602
HpyF10VI GCNNNNNNNGC 3 cut(s) 350, 382, 394
HpyF3I CTNAG 1 cut(s) 239
HpySE526I ACGT 1 cut(s) 84
Hsp92II CATG 1 cut(s) 103
Ksp22I TGATCA 1 cut(s) 43
Kzo9I GATC 4 cut(s) 43, 143, 181, 558
LmnI GCTCC 1 cut(s) 486
LpnPI CCDG 6 cut(s) 234, 297, 324, 444, 524, 545
Lsp1109I GCAGC 2 cut(s) 397, 400
LweI GCATC 3 cut(s) 181, 481, 565
MaeI CTAG 1 cut(s) 234
MaeII ACGT 1 cut(s) 84
MaeIII GTNAC 1 cut(s) 520
MalI GATC 4 cut(s) 45, 145, 183, 560
MboI GATC 4 cut(s) 43, 143, 181, 558
MboII GAAGA 1 cut(s) 92
MhlI GDGCHC 3 cut(s) 126, 297, 483
MlsI TGGCCA 1 cut(s) 252
MluCI AATT 3 cut(s) 173, 445, 549
MluNI TGGCCA 1 cut(s) 252
MlyI GAGTC 1 cut(s) 224
MmeI TCCRAC 1 cut(s) 121
MnlI CCTC 3 cut(s) 168, 465, 505
Mox20I TGGCCA 1 cut(s) 252
MscI TGGCCA 1 cut(s) 252
MslI CAYNNNNRTG 1 cut(s) 58
Msp20I TGGCCA 1 cut(s) 252
MspA1I CMGCKG 1 cut(s) 202
MspR9I CCNGG 1 cut(s) 312
MvaI CCWGG 1 cut(s) 312
MwoI GCNNNNNNNGC 3 cut(s) 350, 382, 394
NdeI CATATG 1 cut(s) 517
NdeII GATC 4 cut(s) 43, 143, 181, 558
NlaIII CATG 1 cut(s) 103
NmuCI GTSAC 1 cut(s) 520
PfeI GAWTC 2 cut(s) 455, 535
PkrI GCNGC 2 cut(s) 387, 390
PleI GAGTC 1 cut(s) 223
PpsI GAGTC 1 cut(s) 223
Psp124BI GAGCTC 1 cut(s) 483
Psp6I CCWGG 1 cut(s) 310
PspGI CCWGG 1 cut(s) 310
RsaI GTAC 1 cut(s) 424
RsaNI GTAC 1 cut(s) 423
RseI CAYNNNNRTG 1 cut(s) 58
SacI GAGCTC 1 cut(s) 483
SatI GCNGC 2 cut(s) 386, 389
Sau3AI GATC 4 cut(s) 43, 143, 181, 558
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 1 cut(s) 312
SduI GDGCHC 3 cut(s) 126, 297, 483
SetI ASST 8 cut(s) 27, 87, 132, 248, 313, 360, 483, 534
SfaNI GCATC 3 cut(s) 181, 481, 565
SmiMI CAYNNNNRTG 1 cut(s) 58
SmlI CTYRAG 2 cut(s) 105, 476
SmoI CTYRAG 2 cut(s) 105, 476
Sse9I AATT 3 cut(s) 173, 445, 549
SsiI CCGC 1 cut(s) 200
SspI AATATT 1 cut(s) 287
SspMI CTAG 1 cut(s) 234
SstI GAGCTC 1 cut(s) 483
StyD4I CCNGG 1 cut(s) 310
StyI CCWWGG 1 cut(s) 570
TaaI ACNGT 2 cut(s) 329, 422
TaiI ACGT 1 cut(s) 87
TasI AATT 3 cut(s) 173, 445, 549
TatI WGTACW 1 cut(s) 422
TfiI GAWTC 2 cut(s) 455, 535
TscAI CASTG 2 cut(s) 334, 383
TseFI GTSAC 1 cut(s) 520
TseI GCWGC 2 cut(s) 385, 388
Tsp45I GTSAC 1 cut(s) 520
TspDTI ATGAA 1 cut(s) 88
TspRI CASTG 2 cut(s) 334, 383
VneI GTGCAC 1 cut(s) 122
XagI CCTNNNNNAGG 1 cut(s) 315
XapI RAATTY 2 cut(s) 173, 549
XspI CTAG 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.