MD11G1228200.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr11
OG0009516
Physical Location & Seq
Reverse (-)
33195768 .. 33198324
2557 bp
Loading structure...
UTR
Exon/CDS
Intron
MD11G1228200.v1.1.491

Sequence Viewer

Length: 621 bp
ATGACTACTACTACTACTAGTAGGAAGCAGCTTGAAGTCGTAAGAGACGATCAAACTCCGCAGAAATGGTGTGTTTTATTGAGGGAAGATGTGTTCAGAAGATTCATGGCTCAAGGCAGCACAAATCCAACAGTGCAGAAGGTCTTTGGTGGTGCATCTTTTTTCAGTCCTTTGCTGTTTGGGAAATTTTTTGATCCTTCTGATGCCTTCCCACTGTGGGAGTTTGAGTCGGATATCTTGCTGTCTGGTCTCAGAAGCTCTGGCCAAACCTGTACTGATTGGTTTCAAACAGATCAAGACTATGTCCTAAAAGTAGAACTACCAGAGGAAGGGAAAAATAATGTTCAAGTGTCTGCTGAAAATGGAAAAGTGGTGGAAATAAGTGGGCAGTGGAAGCAGCAGCAAAGAGGAGAGTCCAACTCCTTGAAGAGCACGAAGGATTGGAGAAGTGGGAATTGGTGGGAACATGGTTATGTTCGGAGGCTTGAGCTCCCACAAGATGCTGATTCCAGAAGGATAGAGGCTTCTCTCGCTAATGACCTACTTTTGGAACTCAAAGTTCACAAGATCAACAAACCCTTGGATTCTAATGCCAATGCAAAAGATAAAGATCCAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

207

Amino Acids

23.6

Weight (kDa)

5.54

Isoelectric Point (pI)

47.0

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 93 - 193 2.5e-09 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 1 cut(s) 59
AclWI GGATC 2 cut(s) 188, 605
AcoI YGGCCR 1 cut(s) 262
AcsI RAATTY 1 cut(s) 185
AfaI GTAC 1 cut(s) 274
AfiI CCNNNNNNNGG 3 cut(s) 217, 329, 547
AgsI TTSAA 4 cut(s) 35, 287, 347, 427
AhlI ACTAGT 1 cut(s) 17
AjuI GAANNNNNNNTTGG 2 cut(s) 439, 471
AloI GAACNNNNNNTCC 2 cut(s) 77, 109
AluBI AGCT 3 cut(s) 31, 258, 490
AluI AGCT 3 cut(s) 31, 258, 490
Alw21I GWGCWC 2 cut(s) 434, 492
Alw26I GTCTC 2 cut(s) 39, 254
AlwI GGATC 2 cut(s) 188, 605
AoxI GGCC 1 cut(s) 262
ApeKI GCWGC 4 cut(s) 28, 117, 397, 400
ApoI RAATTY 1 cut(s) 185
BalI TGGCCA 1 cut(s) 264
BanII GRGCYC 1 cut(s) 492
Bbv12I GWGCWC 2 cut(s) 434, 492
BbvI GCAGC 4 cut(s) 40, 129, 409, 412
BcoDI GTCTC 2 cut(s) 39, 254
BcuI ACTAGT 1 cut(s) 17
BfaI CTAG 1 cut(s) 18
BisI GCNGC 4 cut(s) 29, 118, 398, 401
BlsI GCNGC 4 cut(s) 30, 119, 399, 402
BmsI GCATC 3 cut(s) 164, 193, 490
BplI GAGNNNNNCTC 2 cut(s) 404, 436
BpuEI CTTGAG 2 cut(s) 96, 506
BsaBI GATNNNNATC 1 cut(s) 609
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 1 cut(s) 579
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bsc4I CCNNNNNNNGG 3 cut(s) 217, 329, 547
Bse8I GATNNNNATC 1 cut(s) 609
BseDI CCNNGG 1 cut(s) 579
BseJI GATNNNNATC 1 cut(s) 609
BseLI CCNNNNNNNGG 3 cut(s) 217, 329, 547
BseMII CTCAG 1 cut(s) 265
BseRI GAGGAG 1 cut(s) 423
BseXI GCAGC 4 cut(s) 40, 129, 409, 412
BsgI GTGCAG 1 cut(s) 155
BshFI GGCC 1 cut(s) 264
BsiHKAI GWGCWC 2 cut(s) 434, 492
BslI CCNNNNNNNGG 3 cut(s) 217, 329, 547
BsmAI GTCTC 2 cut(s) 39, 254
BsmBI CGTCTC 1 cut(s) 39
BsnI GGCC 1 cut(s) 264
Bso31I GGTCTC 1 cut(s) 254
Bsp1286I GDGCHC 2 cut(s) 434, 492
Bsp143I GATC 5 cut(s) 49, 193, 292, 567, 610
BspACI CCGC 1 cut(s) 59
BspANI GGCC 1 cut(s) 264
BspCNI CTCAG 1 cut(s) 264
BspPI GGATC 2 cut(s) 188, 605
BspQI GCTCTTC 1 cut(s) 422
BspTNI GGTCTC 1 cut(s) 254
BssECI CCNNGG 1 cut(s) 579
BssMI GATC 5 cut(s) 49, 193, 292, 567, 610
BssT1I CCWWGG 1 cut(s) 579
Bst4CI ACNGT 2 cut(s) 133, 216
Bst6I CTCTTC 1 cut(s) 422
BstDEI CTNAG 1 cut(s) 251
BstKTI GATC 5 cut(s) 52, 196, 295, 570, 613
BstMAI GTCTC 2 cut(s) 39, 254
BstMBI GATC 5 cut(s) 49, 193, 292, 567, 610
BstMWI GCNNNNNNNGC 2 cut(s) 394, 530
BstV1I GCAGC 4 cut(s) 40, 129, 409, 412
BstX2I RGATCY 1 cut(s) 610
BstYI RGATCY 1 cut(s) 610
BsuRI GGCC 1 cut(s) 264
BtsI GCAGTG 1 cut(s) 395
BtsIMutI CAGTG 3 cut(s) 138, 212, 395
Csp6I GTAC 1 cut(s) 273
CviAII CATG 2 cut(s) 106, 467
CviJI RGCY 7 cut(s) 31, 110, 258, 264, 484, 490, 524
CviKI_1 RGCY 7 cut(s) 31, 110, 258, 264, 484, 490, 524
CviQI GTAC 1 cut(s) 273
DdeI CTNAG 1 cut(s) 251
DpnI GATC 5 cut(s) 51, 195, 294, 569, 612
DpnII GATC 5 cut(s) 49, 193, 292, 567, 610
EaeI YGGCCR 1 cut(s) 262
Eam1104I CTCTTC 1 cut(s) 422
EarI CTCTTC 1 cut(s) 422
Ecl136II GAGCTC 1 cut(s) 490
Eco130I CCWWGG 1 cut(s) 579
Eco24I GRGCYC 1 cut(s) 492
Eco31I GGTCTC 1 cut(s) 254
Eco32I GATATC 1 cut(s) 235
Eco53kI GAGCTC 1 cut(s) 490
EcoICRI GAGCTC 1 cut(s) 490
EcoRV GATATC 1 cut(s) 235
EcoT14I CCWWGG 1 cut(s) 579
EcoT38I GRGCYC 1 cut(s) 492
ErhI CCWWGG 1 cut(s) 579
Esp3I CGTCTC 1 cut(s) 39
FaeI CATG 2 cut(s) 109, 470
FaiI YATR 4 cut(s) 107, 303, 468, 474
FatI CATG 2 cut(s) 105, 466
Fnu4HI GCNGC 4 cut(s) 29, 118, 398, 401
FriOI GRGCYC 1 cut(s) 492
Fsp4HI GCNGC 4 cut(s) 29, 118, 398, 401
FspBI CTAG 1 cut(s) 18
GluI GCNGC 4 cut(s) 29, 118, 398, 401
HaeIII GGCC 1 cut(s) 264
Hin1II CATG 2 cut(s) 109, 470
HinfI GANTC 5 cut(s) 102, 227, 413, 506, 584
Hpy166II GTNNAC 1 cut(s) 562
Hpy188I TCNGA 5 cut(s) 98, 202, 232, 254, 480
Hpy188III TCNNGA 2 cut(s) 296, 510
Hpy8I GTNNAC 1 cut(s) 562
HpyAV CCTTC 6 cut(s) 133, 207, 217, 323, 430, 507
HpyCH4III ACNGT 2 cut(s) 133, 216
HpyCH4V TGCA 3 cut(s) 136, 155, 599
HpyF10VI GCNNNNNNNGC 2 cut(s) 394, 530
HpyF3I CTNAG 1 cut(s) 251
Hsp92II CATG 2 cut(s) 109, 470
Kzo9I GATC 5 cut(s) 49, 193, 292, 567, 610
LguI GCTCTTC 1 cut(s) 422
LmnI GCTCC 1 cut(s) 495
LpnPI CCDG 5 cut(s) 231, 246, 283, 336, 523
Lsp1109I GCAGC 4 cut(s) 40, 129, 409, 412
LweI GCATC 3 cut(s) 164, 193, 490
MaeI CTAG 1 cut(s) 18
MalI GATC 5 cut(s) 51, 195, 294, 569, 612
MboI GATC 5 cut(s) 49, 193, 292, 567, 610
MboII GAAGA 3 cut(s) 98, 111, 439
MflI RGATCY 1 cut(s) 610
MhlI GDGCHC 2 cut(s) 434, 492
MlsI TGGCCA 1 cut(s) 264
MluCI AATT 2 cut(s) 185, 454
MluNI TGGCCA 1 cut(s) 264
MlyI GAGTC 2 cut(s) 236, 422
MmeI TCCRAC 3 cut(s) 152, 210, 441
MnlI CCTC 5 cut(s) 75, 319, 401, 474, 514
Mox20I TGGCCA 1 cut(s) 264
MscI TGGCCA 1 cut(s) 264
MslI CAYNNNNRTG 1 cut(s) 471
Msp20I TGGCCA 1 cut(s) 264
MwoI GCNNNNNNNGC 2 cut(s) 394, 530
NdeII GATC 5 cut(s) 49, 193, 292, 567, 610
NlaIII CATG 2 cut(s) 109, 470
PciSI GCTCTTC 1 cut(s) 422
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PfeI GAWTC 3 cut(s) 102, 506, 584
PflFI GACNNNGTC 1 cut(s) 302
PkrI GCNGC 4 cut(s) 30, 119, 399, 402
PleI GAGTC 2 cut(s) 235, 421
PpsI GAGTC 2 cut(s) 235, 421
Psp124BI GAGCTC 1 cut(s) 492
PsuI RGATCY 1 cut(s) 610
PsyI GACNNNGTC 1 cut(s) 302
RsaI GTAC 1 cut(s) 274
RsaNI GTAC 1 cut(s) 273
RseI CAYNNNNRTG 1 cut(s) 471
SacI GAGCTC 1 cut(s) 492
SapI GCTCTTC 1 cut(s) 422
SatI GCNGC 4 cut(s) 29, 118, 398, 401
Sau3AI GATC 5 cut(s) 49, 193, 292, 567, 610
SchI GAGTC 2 cut(s) 236, 422
SduI GDGCHC 2 cut(s) 434, 492
SetI ASST 6 cut(s) 33, 144, 260, 272, 492, 543
SfaNI GCATC 3 cut(s) 164, 193, 490
SmiMI CAYNNNNRTG 1 cut(s) 471
SmlI CTYRAG 2 cut(s) 111, 485
SmoI CTYRAG 2 cut(s) 111, 485
SpeI ACTAGT 1 cut(s) 17
Sse9I AATT 2 cut(s) 185, 454
SsiI CCGC 1 cut(s) 59
SspMI CTAG 1 cut(s) 18
SstI GAGCTC 1 cut(s) 492
StyI CCWWGG 1 cut(s) 579
TaaI ACNGT 2 cut(s) 133, 216
TasI AATT 2 cut(s) 185, 454
TatI WGTACW 1 cut(s) 272
TfiI GAWTC 3 cut(s) 102, 506, 584
TscAI CASTG 3 cut(s) 138, 219, 395
TseI GCWGC 4 cut(s) 28, 117, 397, 400
TspDTI ATGAA 1 cut(s) 94
TspRI CASTG 3 cut(s) 138, 219, 395
Tth111I GACNNNGTC 1 cut(s) 302
XapI RAATTY 1 cut(s) 185
XspI CTAG 1 cut(s) 18
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.