Rorug05G0161200

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0004133
Physical Location & Seq
Forward (+)
15004378 .. 15007039
2662 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0161200.1

Sequence Viewer

Length: 1131 bp
ATGTGTTCCTCTAAACCAAAATTGCAGCAAAGTCCTAGTGTTACTCCAACAACACCTAAAACCAATCGCCGTTTGGTTCTTCAGCCAACTGGTAACCAATTTCCAAGCTTGGAACAAAGGAAGTTTCTCAAAAAGACCTCCCATGAATCCCTTATTCCACCTCCTTTGCTATCACCACTGCCATCACCGAAAACTAAGCCCGCAATTTCTCCTCCCATCTCTCCCAAGTTACCTTCGCCTCGACCACCAGCACTTAAGAGAGGAAAAGACCATAATGAGCTCAATTCTAGTCTTGAGAAGTTAATTCTCACACCTAAGTCAACAACCAAGTTGACTGGTTCAGTGAAAAAGTCAAAGAGATCAAGTGTTGCAGGTGCAGCTTCTGCTGAAAATGTAATGAAAAATATATCATCTTTGATAGTAGAGGCACCAGGAAGCATAGCAGCTGCAAGGAGAGAGCAAGTTGCAACAATGCAAGAACAACGAAAGCTCAGAATTGCGCATTATGGAAGAACAAAGTCTGCAAAGTTTGAAGGGAAAGTAGTGCCTCTTGATACTTCATCCACCAATGTTGAACAAAGGAGATGCAGTTTTATCACACCAAATTCAGACCCTCTGTATGTTGCTTACCATGATCAAGAATGGGGAGTTCCTGTTCATGATGACAATTTGTTGCTTGAATTGCTACTGTTGACCAGTGCTCAAGTAGGATCAGATTGGAGCTCGGTCCTGAGGAAAAGGCAGGCTTTGAGGGAGGCATTTTCAAGTTTTGATGCAGAAATTGTGGCTAAGTTTAATGAAAAGAAGATAACTTCAGTAAGTGCTGATACTGGTACTGATATAAGCTTGATCAGAGGAGTTGTTGACAATGCTAAAAGAATTCTTGAGATTAAAAGGGAAACTGGGTCATTTGACAACTACTTGTGGGGATTTGTGAATCACAAGCCAATTTCCACCCAGTACAAGTCATGCCACAAGATCCCTGTGAAGACCTCCAAATCAGAGTCCATAAGCAAAGCCATGGTGAAGAAGGGTTTCAGGTTTGTGGGCCCCACTGTGATTCACTCCTTCATGCAAGCTGCTGGCCTCACTAATGACCACTTGATCACTTGCTCGAGGCACCAACTATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

376

Amino Acids

41.5

Weight (kDa)

10.03

Isoelectric Point (pI)

48.8

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Adenine_glyco PF03352 202 - 374 9.5e-64 Methyladenine glycosylase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 362
Acc16I TGCGCA 1 cut(s) 501
Acc36I ACCTGC 1 cut(s) 362
AccB1I GGYRCC 2 cut(s) 427, 1119
AciI CCGC 1 cut(s) 201
AclWI GGATC 2 cut(s) 718, 973
AcsI RAATTY 2 cut(s) 604, 879
AcuI CTGAAG 2 cut(s) 65, 798
AfaI GTAC 2 cut(s) 835, 962
AflII CTTAAG 1 cut(s) 254
AgsI TTSAA 4 cut(s) 533, 575, 680, 765
AjnI CCWGG 1 cut(s) 430
AloI GAACNNNNNNTCC 2 cut(s) 639, 671
AluBI AGCT 8 cut(s) 108, 280, 380, 446, 490, 723, 846, 1079
AluI AGCT 8 cut(s) 108, 280, 380, 446, 490, 723, 846, 1079
Alw21I GWGCWC 3 cut(s) 282, 703, 725
AlwI GGATC 2 cut(s) 718, 973
AlwNI CAGNNNCTG 1 cut(s) 383
Ama87I CYCGRG 1 cut(s) 1114
AoxI GGCC 2 cut(s) 1048, 1084
ApaI GGGCCC 1 cut(s) 1052
ApeKI GCWGC 5 cut(s) 25, 377, 443, 446, 1079
ApoI RAATTY 2 cut(s) 604, 879
Asp700I GAANNNNTTC 1 cut(s) 1034
AspLEI GCGC 1 cut(s) 502
AspS9I GGNCC 3 cut(s) 727, 1048, 1049
AsuHPI GGTGA 3 cut(s) 165, 177, 1036
AvaI CYCGRG 1 cut(s) 1114
AvaII GGWCC 1 cut(s) 727
AxyI CCTNAGG 1 cut(s) 731
BaeGI GKGCMC 1 cut(s) 1052
BanI GGYRCC 2 cut(s) 427, 1119
BanII GRGCYC 3 cut(s) 282, 725, 1052
BbsI GAAGAC 1 cut(s) 995
Bbv12I GWGCWC 3 cut(s) 282, 703, 725
BbvI GCAGC 5 cut(s) 37, 389, 433, 455, 1066
BccI CCATC 2 cut(s) 190, 224
BceAI ACGGC 1 cut(s) 54
BciT130I CCWGG 1 cut(s) 432
BclI TGATCA 3 cut(s) 634, 849, 1104
BfaI CTAG 2 cut(s) 36, 288
BfmI CTRYAG 1 cut(s) 1127
BfrI CTTAAG 1 cut(s) 254
BfuAI ACCTGC 1 cut(s) 362
BisI GCNGC 5 cut(s) 26, 378, 444, 447, 1080
BlsI GCNGC 5 cut(s) 27, 379, 445, 448, 1081
Bme1390I CCNGG 1 cut(s) 432
Bme18I GGWCC 1 cut(s) 727
BmeT110I CYCGRG 1 cut(s) 1114
BmgT120I GGNCC 3 cut(s) 727, 1048, 1049
BmiI GGNNCC 4 cut(s) 429, 1050, 1051, 1121
BmrFI CCNGG 1 cut(s) 432
BmrI ACTGGG 2 cut(s) 912, 952
BmsI GCATC 2 cut(s) 575, 763
BmuI ACTGGG 2 cut(s) 912, 952
BpiI GAAGAC 1 cut(s) 995
BpuEI CTTGAG 3 cut(s) 314, 687, 905
BsaJI CCNNGG 1 cut(s) 1020
BsaXI ACNNNNNCTCC 4 cut(s) 574, 604, 639, 669
Bse1I ACTGG 6 cut(s) 94, 340, 696, 835, 907, 958
Bse21I CCTNAGG 1 cut(s) 731
BseBI CCWGG 1 cut(s) 432
BseDI CCNNGG 1 cut(s) 1020
BseGI GGATG 1 cut(s) 560
BseMII CTCAG 2 cut(s) 505, 722
BseNI ACTGG 6 cut(s) 94, 340, 696, 835, 907, 958
BseRI GAGGAG 2 cut(s) 201, 870
BseSI GKGCMC 1 cut(s) 1052
BseXI GCAGC 5 cut(s) 37, 389, 433, 455, 1066
BsgI GTGCAG 1 cut(s) 396
BshFI GGCC 2 cut(s) 1050, 1086
BshNI GGYRCC 2 cut(s) 427, 1119
BsiHKAI GWGCWC 3 cut(s) 282, 703, 725
BsiHKCI CYCGRG 1 cut(s) 1114
BsnI GGCC 2 cut(s) 1050, 1086
BsoBI CYCGRG 1 cut(s) 1114
Bsp120I GGGCCC 1 cut(s) 1048
Bsp1286I GDGCHC 4 cut(s) 282, 703, 725, 1052
Bsp143I GATC 6 cut(s) 359, 634, 710, 849, 978, 1104
Bsp19I CCATGG 1 cut(s) 1020
BspACI CCGC 1 cut(s) 201
BspANI GGCC 2 cut(s) 1050, 1086
BspCNI CTCAG 2 cut(s) 504, 723
BspHI TCATGA 1 cut(s) 658
BspLI GGNNCC 4 cut(s) 429, 1050, 1051, 1121
BspMI ACCTGC 1 cut(s) 362
BspPI GGATC 2 cut(s) 718, 973
BspT107I GGYRCC 2 cut(s) 427, 1119
BspTI CTTAAG 1 cut(s) 254
BsrI ACTGG 6 cut(s) 94, 340, 696, 835, 907, 958
BssECI CCNNGG 1 cut(s) 1020
BssMI GATC 6 cut(s) 359, 634, 710, 849, 978, 1104
BssT1I CCWWGG 1 cut(s) 1020
Bst2UI CCWGG 1 cut(s) 432
Bst4CI ACNGT 2 cut(s) 690, 1057
BstAFI CTTAAG 1 cut(s) 254
BstAPI GCANNNNNTGC 1 cut(s) 383
BstC8I GCNNGC 4 cut(s) 201, 744, 1077, 1084
BstDEI CTNAG 5 cut(s) 195, 315, 491, 731, 789
BstDSI CCRYGG 1 cut(s) 1020
BstEII GGTNACC 1 cut(s) 92
BstF5I GGATG 1 cut(s) 560
BstHHI GCGC 1 cut(s) 502
BstKTI GATC 6 cut(s) 362, 637, 713, 852, 981, 1107
BstMBI GATC 6 cut(s) 359, 634, 710, 849, 978, 1104
BstMWI GCNNNNNNNGC 3 cut(s) 377, 383, 682
BstNI CCWGG 1 cut(s) 432
BstPI GGTNACC 1 cut(s) 92
BstSCI CCNGG 1 cut(s) 430
BstSFI CTRYAG 1 cut(s) 1127
BstSLI GKGCMC 1 cut(s) 1052
BstV1I GCAGC 5 cut(s) 37, 389, 433, 455, 1066
BstV2I GAAGAC 1 cut(s) 995
BstX2I RGATCY 1 cut(s) 978
BstYI RGATCY 1 cut(s) 978
Bsu36I CCTNAGG 1 cut(s) 731
BsuRI GGCC 2 cut(s) 1050, 1086
BtgI CCRYGG 1 cut(s) 1020
BtsCI GGATG 1 cut(s) 560
BtsI GCAGTG 1 cut(s) 176
BtsIMutI CAGTG 4 cut(s) 176, 348, 703, 1053
BveI ACCTGC 1 cut(s) 362
Cac8I GCNNGC 4 cut(s) 201, 744, 1077, 1084
CaiI CAGNNNCTG 1 cut(s) 383
CciI TCATGA 1 cut(s) 658
CfoI GCGC 1 cut(s) 502
Cfr13I GGNCC 3 cut(s) 727, 1048, 1049
Csp6I GTAC 2 cut(s) 834, 961
CspCI CAANNNNNGTGG 4 cut(s) 147, 182, 553, 588
CviAII CATG 6 cut(s) 143, 632, 659, 969, 1021, 1072
CviQI GTAC 2 cut(s) 834, 961
DdeI CTNAG 5 cut(s) 195, 315, 491, 731, 789
DpnI GATC 6 cut(s) 361, 636, 712, 851, 980, 1106
DpnII GATC 6 cut(s) 359, 634, 710, 849, 978, 1104
Ecl136II GAGCTC 2 cut(s) 280, 723
Eco130I CCWWGG 1 cut(s) 1020
Eco24I GRGCYC 3 cut(s) 282, 725, 1052
Eco47I GGWCC 1 cut(s) 727
Eco53kI GAGCTC 2 cut(s) 280, 723
Eco57I CTGAAG 2 cut(s) 65, 798
Eco81I CCTNAGG 1 cut(s) 731
Eco88I CYCGRG 1 cut(s) 1114
Eco91I GGTNACC 1 cut(s) 92
EcoICRI GAGCTC 2 cut(s) 280, 723
EcoO109I RGGNCCY 1 cut(s) 1049
EcoO65I GGTNACC 1 cut(s) 92
EcoRI GAATTC 1 cut(s) 879
EcoRII CCWGG 1 cut(s) 430
EcoT14I CCWWGG 1 cut(s) 1020
EcoT38I GRGCYC 3 cut(s) 282, 725, 1052
ErhI CCWWGG 1 cut(s) 1020
FaeI CATG 6 cut(s) 146, 635, 662, 972, 1024, 1075
FalI AAGNNNNNCTT 2 cut(s) 730, 762
FatI CATG 6 cut(s) 142, 631, 658, 968, 1020, 1071
FauI CCCGC 1 cut(s) 208
FbaI TGATCA 3 cut(s) 634, 849, 1104
Fnu4HI GCNGC 5 cut(s) 26, 378, 444, 447, 1080
FokI GGATG 1 cut(s) 547
FriOI GRGCYC 3 cut(s) 282, 725, 1052
Fsp4HI GCNGC 5 cut(s) 26, 378, 444, 447, 1080
FspBI CTAG 2 cut(s) 36, 288
FspI TGCGCA 1 cut(s) 501
GlaI GCGC 1 cut(s) 501
GluI GCNGC 5 cut(s) 26, 378, 444, 447, 1080
HaeIII GGCC 2 cut(s) 1050, 1086
HhaI GCGC 1 cut(s) 502
Hin1II CATG 6 cut(s) 146, 635, 662, 972, 1024, 1075
Hin6I GCGC 1 cut(s) 500
HinP1I GCGC 1 cut(s) 500
HincII GTYRAC 4 cut(s) 321, 333, 693, 865
HindII GTYRAC 4 cut(s) 321, 333, 693, 865
HindIII AAGCTT 2 cut(s) 106, 844
HinfI GANTC 4 cut(s) 146, 937, 1004, 1060
HphI GGTGA 3 cut(s) 165, 177, 1036
Hpy166II GTNNAC 4 cut(s) 321, 333, 693, 865
Hpy188I TCNGA 5 cut(s) 494, 610, 715, 854, 1003
Hpy188III TCNNGA 6 cut(s) 293, 551, 638, 659, 730, 884
Hpy8I GTNNAC 4 cut(s) 321, 333, 693, 865
HpyAV CCTTC 4 cut(s) 243, 527, 1024, 1078
HpyCH4III ACNGT 2 cut(s) 690, 1057
HpyF10VI GCNNNNNNNGC 3 cut(s) 377, 383, 682
HpyF3I CTNAG 5 cut(s) 195, 315, 491, 731, 789
Hsp92II CATG 6 cut(s) 146, 635, 662, 972, 1024, 1075
HspAI GCGC 1 cut(s) 500
Ksp22I TGATCA 3 cut(s) 634, 849, 1104
Kzo9I GATC 6 cut(s) 359, 634, 710, 849, 978, 1104
LmnI GCTCC 1 cut(s) 720
Lsp1109I GCAGC 5 cut(s) 37, 389, 433, 455, 1066
LweI GCATC 2 cut(s) 575, 763
MaeI CTAG 2 cut(s) 36, 288
MaeIII GTNAC 3 cut(s) 40, 92, 228
MalI GATC 6 cut(s) 361, 636, 712, 851, 980, 1106
MboI GATC 6 cut(s) 359, 634, 710, 849, 978, 1104
MboII GAAGA 5 cut(s) 71, 522, 817, 1000, 1039
MflI RGATCY 1 cut(s) 978
MhlI GDGCHC 4 cut(s) 282, 703, 725, 1052
MlyI GAGTC 1 cut(s) 1013
MmeI TCCRAC 1 cut(s) 71
MroXI GAANNNNTTC 1 cut(s) 1034
MseI TTAA 4 cut(s) 255, 302, 795, 891
MspA1I CMGCKG 1 cut(s) 446
MspCI CTTAAG 1 cut(s) 254
MspR9I CCNGG 1 cut(s) 432
MvaI CCWGG 1 cut(s) 432
MwoI GCNNNNNNNGC 3 cut(s) 377, 383, 682
NcoI CCATGG 1 cut(s) 1020
NdeII GATC 6 cut(s) 359, 634, 710, 849, 978, 1104
NlaIII CATG 6 cut(s) 146, 635, 662, 972, 1024, 1075
NlaIV GGNNCC 4 cut(s) 429, 1050, 1051, 1121
NsbI TGCGCA 1 cut(s) 501
PaeR7I CTCGAG 1 cut(s) 1114
PagI TCATGA 1 cut(s) 658
PaqCI CACCTGC 1 cut(s) 362
PdmI GAANNNNTTC 1 cut(s) 1034
PfeI GAWTC 3 cut(s) 146, 937, 1060
PkrI GCNGC 5 cut(s) 27, 379, 445, 448, 1081
PleI GAGTC 1 cut(s) 1012
PpsI GAGTC 1 cut(s) 1012
Psp124BI GAGCTC 2 cut(s) 282, 725
Psp6I CCWGG 1 cut(s) 430
PspEI GGTNACC 1 cut(s) 92
PspGI CCWGG 1 cut(s) 430
PspN4I GGNNCC 4 cut(s) 429, 1050, 1051, 1121
PspOMI GGGCCC 1 cut(s) 1048
PspPI GGNCC 3 cut(s) 727, 1048, 1049
PspXI VCTCGAGB 1 cut(s) 1114
PstNI CAGNNNCTG 1 cut(s) 383
PsuI RGATCY 1 cut(s) 978
PvuII CAGCTG 1 cut(s) 446
RsaI GTAC 2 cut(s) 835, 962
RsaNI GTAC 2 cut(s) 834, 961
SacI GAGCTC 2 cut(s) 282, 725
SaqAI TTAA 4 cut(s) 255, 302, 795, 891
SatI GCNGC 5 cut(s) 26, 378, 444, 447, 1080
Sau3AI GATC 6 cut(s) 359, 634, 710, 849, 978, 1104
Sau96I GGNCC 3 cut(s) 727, 1048, 1049
SchI GAGTC 1 cut(s) 1013
ScrFI CCNGG 1 cut(s) 432
SduI GDGCHC 4 cut(s) 282, 703, 725, 1052
SfaNI GCATC 2 cut(s) 575, 763
SfcI CTRYAG 1 cut(s) 1127
Sfr274I CTCGAG 1 cut(s) 1114
SinI GGWCC 1 cut(s) 727
SlaI CTCGAG 1 cut(s) 1114
SmlI CTYRAG 5 cut(s) 254, 293, 702, 884, 1114
SmoI CTYRAG 5 cut(s) 254, 293, 702, 884, 1114
SsiI CCGC 1 cut(s) 201
SspMI CTAG 2 cut(s) 36, 288
SstI GAGCTC 2 cut(s) 282, 725
StyD4I CCNGG 1 cut(s) 430
StyI CCWWGG 1 cut(s) 1020
TaaI ACNGT 2 cut(s) 690, 1057
TaqI TCGA 2 cut(s) 241, 1115
TaqII GACCGA 1 cut(s) 715
TatI WGTACW 1 cut(s) 960
TfiI GAWTC 3 cut(s) 146, 937, 1060
Tru1I TTAA 4 cut(s) 255, 302, 795, 891
Tru9I TTAA 4 cut(s) 255, 302, 795, 891
TscAI CASTG 4 cut(s) 183, 348, 703, 1060
TseI GCWGC 5 cut(s) 25, 377, 443, 446, 1079
TspDTI ATGAA 6 cut(s) 159, 413, 549, 647, 813, 1060
TspRI CASTG 4 cut(s) 183, 348, 703, 1060
Vha464I CTTAAG 1 cut(s) 254
VpaK11BI GGWCC 1 cut(s) 727
XapI RAATTY 2 cut(s) 604, 879
XcmI CCANNNNNNNNNTGG 1 cut(s) 70
XhoI CTCGAG 1 cut(s) 1114
XmnI GAANNNNTTC 1 cut(s) 1034
XspI CTAG 2 cut(s) 36, 288
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.