Basic Information

Type: gene

Biological Identity

Species

arabidopsis_thaliana

Chromosome

Orthogroup ID

OG0004133

Physical Location & Seq

Genomic Coordinates Reverse (-)

22197530 .. 22202127

Gene Structure 4598 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

AT5G54650.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2703 bp

ATGGTTGGAATGATTCGAGGAGGAATGGGTGATCAGAATTGGAGTCGTTTAGTTTTCTGGTTGATTCTGTTTTCTGGGTTATTGGTAATAACTTTGGAAGAGAATCCAGAAAAAGATGAGATTTTTCTTAGTCAATTCATGGCCCCATCAACAGGGCAGGTCAATGAACATATGGAAGAAACATCATGGGCGCAGCGTTGTTGGCAAGATTCAGATTGTGTGAAGGAAGCTGTGGCAGAATTCAATTTATGTTTTCCAGGATCAAAGGATAGTAGGGAACTATTTGGTTTGAACCATACCAATCTGAAGCAGACTCTTCTTGATTGTATCCAAGAAAAAGGAAAGTTAAATGGTCATAACCCCAAGTATCTGGAGCTCTTATCATCTATGCTTGATATTCCGAGGAGAAATCTAGCTACTAAACCCGGCTCTTCCCCTTCCCCTTCACCTTCCCGCCCTCCTAAGCGTAGTCGGGGGCCTCCGCGCCCACCTACTCGACCAAAATCCCCACCTCCTCGGAAATCATCTTTTCCACCATCAAGATCTCCCCCTCCTCCTCCTGCTAAGAAAAACGCTTCTAAAAATTCAACTTCTGCACCTGTTTCACCTGCGAAAAAAAAAGAGGATCATGAGAAAACAATCATCATTGCTGTTGTTGTGACTGCAGTTTCTACGTTTTTGTTGGCTGCATTATTCTTCTTATGCTGCTCCAGGGTTTGTGGAAATGGTTCAGGAGGCAGGAAAAACGATGAAAGGCCTCTTCTCAGTTTAAGCAGTAGTGATTACTCTGTTGGTTCCTCCATCAACTATGGTGGTTCGGTCAAAGGAGATAAGCAGGGTCATCAATCTTTTAATATTTACTCAAACCAAGGAAAAATGTCATCTTTCGATGGAAGTAACTCTGACACTTCAGATTCGTTAGAAGAACGGCTATCACACGAAGGCCTGAGAAATAATAGTATTACCAATCACGGGCTTCCACCTTTGAAGCCTCCACCTGGAAGAACAGCTTCTGTACTTTCAGGGAAGTCTTTTTCTGGAAAAGTGGAGCCTCTTCCACCGGAACCACCCAAATTTCTTAAGGTATCCTCGAAAAAGGCTTCTGCTCCTCCACCGCCAGTTCCAGCACCGCAAATGCCATCTTCTGCTGGTCCTCCACGACCGCCCCCTCCAGCTCCGCCACCTGGTTCTGGTGGGCCTAAGCCACCACCTCCTCCTGGACCAAAAGGACCACGTCCGCCTCCACCAATGAGTCTGGGCCCAAAAGCACCCCGACCGCCTTCTGGGCCAGCAGATGCATTAGATGATGATGCTCCCAAAACAAAGCTGAAGCCATTTTTCTGGGATAAAGTGCAAGCAAACCCAGAACATTCAATGGTTTGGAATGATATAAGATCAGGATCCTTTCAGTTCAATGAAGAGATGATAGAGTCGTTATTTGGATATGCAGCAGCAGACAAGAATAAAAACGACAAGAAGGGGTCCTCTGGACAAGCTGCTTTACCTCAGTTTGTTCAGATCCTCGAACCAAAGAAAGGACAAAATCTATCAATTCTTTTGCGTGCTTTGAATGCGACAACTGAAGAAGTGTGTGATGCACTTCGCGAAGGAAATGAGCTTCCTGTAGAATTCATTCAGACTCTGTTAAAGATGGCACCAACACCAGAAGAAGAACTAAAACTTAGATTATACTGCGGTGAAATAGCTCAACTGGGATCCGCTGAACGATTCCTGAAAGCAGTCGTTGATATCCCTTTTGCTTTCAAGCGTCTAGAAGCACTTCTATTTATGTGTACACTCCATGAAGAAATGGCTTTTGTTAAAGAATCATTTCAGAAATTGGAGGTTGCTTGTAAAGAACTTAGGGGAAGTCGGCTGTTCCTGAAGCTCTTAGAGGCAGTTCTTAAGACCGGAAACCGAATGAATGATGGTACATTTAGGGGTGGTGCTCAAGCTTTCAAGCTGGACACTCTTTTGAAATTAGCAGACGTCAAGGGAACAGATGGGAAAACAACGCTCCTCCACTTTGTTGTTCAAGAGATAATTAGGACTGAAGGTGTAAGAGCGGCTCGTACTATCAGAGAGAGCCAAAGTTTCTCAAGTGTTAAAACAGAGGATCTCCTAGTCGAGGAAACCTCTGAAGAGTCAGAAGAGAATTACCGTAATCTTGGACTTGAGAAAGTTTCAGGCTTGAGCAGTGAGCTTGAACATGTAAAGAAATCTGCAAACATAGATGCCGATGGTTTAACAGGGACTGTTCTTAAAATGGGTCATGCTTTGTCGAAAGCTCGAGACTTTGTCAACTCAGAGATGAAAAGTTCAGGCGAGGAGAGTGGGTTTCGTGAAGCGCTTGAAGATTTTATTCAAAATGCTGAAGGAAGCATCATGTCCATACTCGAGGAAGAGAAGAGAATAATGGCTTTGGTGAAAAGTACAGGGGACTATTTCCATGGCAAAGCAGGAAAAGACGAAGGGTTGCGTTTGTTTGTGATTGTGCGCGATTTTCTGATAATCTTAGATAAATCCTGCAAAGAAGTCAGAGAAGCACGAGGAAGACCCGTAAGGATGGCCAGAAAACAAGGCTCAACAGCCTCAGCATCTTCTGAAACTCCAAGACAAACGCCTTCATTAGATCCTAGGCAGAAGCTGTTTCCAGCTATTACAGAGAGACGCGTGGATCAGTCTAGTTCAGATTCAGACTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0007010 GO:0007015 GO:0007275 GO:0008064 GO:0008092 GO:0008150 GO:0008154 GO:0009524 GO:0009653 GO:0009791 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010638 GO:0016043 GO:0022414 GO:0022607 GO:0030029 GO:0030036 GO:0030041 GO:0030312 GO:0030832 GO:0030833 GO:0030838 GO:0031334 GO:0032271 GO:0032273 GO:0032501 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0033043 GO:0034622 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044424 GO:0044444 GO:0044464 GO:0045010 GO:0048316 GO:0048317 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051258 GO:0051493 GO:0051495 GO:0061458 GO:0065003 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0090066 GO:0090698 GO:0097435 GO:0110053 GO:1902903 GO:1902905

KEGG Pathways

Metabolic & Signaling

ko04320 map04320

Pfam Domains

Protein Families

FH2

Protein Analysis

900

Amino Acids

98.36

Weight (kDa)

8.84

Isoelectric Point (pI)

54.0

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FH2	PF02181	437 - 838	1.1e-109	Formin Homology 2 Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004133)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54650 AT5G54650
fragaria_vesca	FvH4_3g21670 FvH4_3g21670 FvH4_3g21670 FvH4_3g21670 FvH4_3g21670 FvH4_3g21670
malus_domestica	MD03G1213400.v1.1 MD11G1228300.v1.1
prunus_persica	Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1
pyrus_communis	pycom03g16200 pycom11g20090
rosa_chinensis	RchiOBHm_Chr5g0037151
rosa_laevigata	RLG00000033762
rosa_multiflora	Rmu_ssc0000448.1_g000003
rosa_roxburghii	Rroxscaffold_1G00043830
rosa_rugosa	Rorug05G0161100 Rorug05G0161100 Rorug05G0161200
rosa_samantha	Rh5AG252800 Rh5BG254700 Rh5CG287200 Rh5DG263200 Rh6CG204900
rosa_wichuraiana	Rw5G023330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AarI	CACCTGC	1 cut(s)	616
AatII	GACGTC	1 cut(s)	1992
Acc36I	ACCTGC	2 cut(s)	148, 616
AccB1I	GGYRCC	1 cut(s)	1654
AccBSI	CCGCTC	1 cut(s)	2066
AccII	CGCG	4 cut(s)	484, 1605, 2499, 2673
AcoI	YGGCCR	1 cut(s)	2568
AcsI	RAATTY	4 cut(s)	239, 583, 1073, 1628
AcuI	CTGAAG	8 cut(s)	326, 894, 1349, 1602, 1904, 2073, 2160, 2394
AcyI	GRCGYC	1 cut(s)	1989
AfaI	GTAC	5 cut(s)	1017, 1795, 1933, 2074, 2434
AfeI	AGCGCT	1 cut(s)	2349
AfiI	CCNNNNNNNGG	9 cut(s)	152, 972, 998, 1184, 1190, 1217, 1283, 1535, 2128
AflII	CTTAAG	2 cut(s)	1079, 1904
AflIII	ACRYGT	2 cut(s)	2209, 2671
AjiI	CACGTC	1 cut(s)	1235
AjnI	CCWGG	5 cut(s)	256, 710, 997, 1183, 1216
Alw21I	GWGCWC	2 cut(s)	378, 1951
Alw26I	GTCTC	2 cut(s)	2286, 2662
AlwNI	CAGNNNCTG	4 cut(s)	1013, 1642, 2255, 2647
Ama87I	CYCGRG	2 cut(s)	2289, 2396
Aor51HI	AGCGCT	1 cut(s)	2349
AoxI	GGCC	8 cut(s)	141, 476, 755, 943, 1196, 1258, 1286, 2568
ApaI	GGGCCC	1 cut(s)	1262
ApeKI	GCWGC	6 cut(s)	193, 686, 705, 1448, 1451, 1496
ApoI	RAATTY	4 cut(s)	239, 583, 1073, 1628
Asp700I	GAANNNNTTC	5 cut(s)	727, 1009, 1632, 1779, 1830
AspA2I	CCTAGG	1 cut(s)	2636
AspLEI	GCGC	4 cut(s)	193, 486, 2350, 2499
AsuC2I	CCSGG	1 cut(s)	426
AsuHPI	GGTGA	5 cut(s)	41, 438, 597, 1709, 2437
AvaI	CYCGRG	2 cut(s)	2289, 2396
AvaII	GGWCC	4 cut(s)	1151, 1220, 1229, 1482
AvrII	CCTAGG	1 cut(s)	2636
BaeGI	GKGCMC	1 cut(s)	1262
BalI	TGGCCA	1 cut(s)	2570
BamHI	GGATCC	2 cut(s)	1400, 1715
BanI	GGYRCC	1 cut(s)	1654
BanII	GRGCYC	2 cut(s)	378, 1262
BauI	CACGAG	1 cut(s)	2547
BbsI	GAAGAC	1 cut(s)	2560
Bbv12I	GWGCWC	2 cut(s)	378, 1951
BbvCI	CCTCAGC	1 cut(s)	2593
BbvI	GCAGC	6 cut(s)	205, 673, 692, 1460, 1463, 1483
BceAI	ACGGC	1 cut(s)	944
BciT130I	CCWGG	5 cut(s)	258, 712, 999, 1185, 1218
BciVI	GTATCC	2 cut(s)	338, 1096
BclI	TGATCA	1 cut(s)	31
BcnI	CCSGG	1 cut(s)	426
BcoDI	GTCTC	2 cut(s)	2286, 2662
BfaI	CTAG	5 cut(s)	413, 1772, 2123, 2637, 2685
BfmI	CTRYAG	2 cut(s)	663, 1623
BfoI	RGCGCY	1 cut(s)	2351
BfrI	CTTAAG	2 cut(s)	1079, 1904
BfuAI	ACCTGC	2 cut(s)	148, 616
BfuI	GTATCC	2 cut(s)	338, 1096
BglI	GCCNNNNNGGC	1 cut(s)	1285
BglII	AGATCT	1 cut(s)	542
BisI	GCNGC	7 cut(s)	194, 687, 706, 1449, 1452, 1497, 2067
BlnI	CCTAGG	1 cut(s)	2636
BlsI	GCNGC	7 cut(s)	195, 688, 707, 1450, 1453, 1498, 2068
Bme1390I	CCNGG	6 cut(s)	258, 426, 712, 999, 1185, 1218
Bme18I	GGWCC	4 cut(s)	1151, 1220, 1229, 1482
BmeT110I	CYCGRG	2 cut(s)	2289, 2396
BmgBI	CACGTC	1 cut(s)	1235
BmrFI	CCNGG	6 cut(s)	258, 426, 712, 999, 1185, 1218
BmrI	ACTGGG	1 cut(s)	1721
BmsI	GCATC	6 cut(s)	1285, 1300, 1585, 2224, 2391, 2606
BmuI	ACTGGG	1 cut(s)	1721
BpiI	GAAGAC	1 cut(s)	2560
BplI	GAGNNNNNCTC	2 cut(s)	2120, 2152
BpmI	CTGGAG	3 cut(s)	392, 694, 1155
Bpu10I	CCTNAGC	3 cut(s)	462, 1200, 2593
BpuEI	CTTGAG	4 cut(s)	1935, 2083, 2195, 2212
BpuMI	CCSGG	1 cut(s)	426
BsaBI	GATNNNNATC	1 cut(s)	1399
BsaHI	GRCGYC	1 cut(s)	1989
BsaJI	CCNNGG	6 cut(s)	401, 515, 711, 868, 2449, 2636
BsaWI	WCCGGW	2 cut(s)	1060, 1910
BsaXI	ACNNNNNCTCC	2 cut(s)	2321, 2351
Bsc4I	CCNNNNNNNGG	9 cut(s)	152, 972, 998, 1184, 1190, 1217, 1283, 1535, 2128
Bse1I	ACTGG	2 cut(s)	1118, 1716
Bse3DI	GCAATG	1 cut(s)	645
Bse8I	GATNNNNATC	1 cut(s)	1399
BseBI	CCWGG	5 cut(s)	258, 712, 999, 1185, 1218
BseDI	CCNNGG	6 cut(s)	401, 515, 711, 868, 2449, 2636
BseGI	GGATG	1 cut(s)	2571
BseJI	GATNNNNATC	1 cut(s)	1399
BseLI	CCNNNNNNNGG	9 cut(s)	152, 972, 998, 1184, 1190, 1217, 1283, 1535, 2128
BseMI	GCAATG	1 cut(s)	645
BseMII	CTCAG	5 cut(s)	778, 938, 1520, 2319, 2607
BseNI	ACTGG	2 cut(s)	1118, 1716
BseRI	GAGGAG	9 cut(s)	33, 418, 504, 543, 546, 1098, 1203, 2009, 2342
BseSI	GKGCMC	1 cut(s)	1262
BseXI	GCAGC	6 cut(s)	205, 673, 692, 1460, 1463, 1483
BsgI	GTGCAG	1 cut(s)	579
Bsh1236I	CGCG	4 cut(s)	484, 1605, 2499, 2673
Bsh1285I	CGRYCG	2 cut(s)	1163, 1277
BshFI	GGCC	8 cut(s)	143, 478, 757, 945, 1198, 1260, 1288, 2570
BshNI	GGYRCC	1 cut(s)	1654
BsiEI	CGRYCG	2 cut(s)	1163, 1277
BsiHKAI	GWGCWC	2 cut(s)	378, 1951
BsiHKCI	CYCGRG	2 cut(s)	2289, 2396
BsiSI	CCGG	3 cut(s)	426, 1061, 1911
BslFI	GGGAC	2 cut(s)	2266, 2453
BslI	CCNNNNNNNGG	9 cut(s)	152, 972, 998, 1184, 1190, 1217, 1283, 1535, 2128
BsmAI	GTCTC	2 cut(s)	2286, 2662
BsmBI	CGTCTC	1 cut(s)	2662
BsmFI	GGGAC	2 cut(s)	2266, 2453
BsmI	GAATGC	1 cut(s)	1576
BsnI	GGCC	8 cut(s)	143, 478, 757, 945, 1198, 1260, 1288, 2570
BsoBI	CYCGRG	2 cut(s)	2289, 2396
Bsp120I	GGGCCC	1 cut(s)	1258
Bsp1286I	GDGCHC	3 cut(s)	378, 1262, 1951
Bsp1407I	TGTACA	1 cut(s)	1793
Bsp19I	CCATGG	1 cut(s)	2449
Bsp68I	TCGCGA	1 cut(s)	1605
BspANI	GGCC	8 cut(s)	143, 478, 757, 945, 1198, 1260, 1288, 2570
BspCNI	CTCAG	5 cut(s)	777, 939, 1519, 2318, 2606
BspFNI	CGCG	4 cut(s)	484, 1605, 2499, 2673
BspHI	TCATGA	1 cut(s)	628
BspMAI	CTGCAG	1 cut(s)	667
BspMI	ACCTGC	2 cut(s)	148, 616
BspQI	GCTCTTC	1 cut(s)	436
BspT107I	GGYRCC	1 cut(s)	1654
BspTI	CTTAAG	2 cut(s)	1079, 1904
BsrBI	CCGCTC	1 cut(s)	2066
BsrDI	GCAATG	1 cut(s)	645
BsrGI	TGTACA	1 cut(s)	1793
BsrI	ACTGG	2 cut(s)	1118, 1716
BssECI	CCNNGG	6 cut(s)	401, 515, 711, 868, 2449, 2636
BssNI	GRCGYC	1 cut(s)	1989
BssSI	CACGAG	1 cut(s)	2547
BssT1I	CCWWGG	3 cut(s)	868, 2449, 2636
Bst2BI	CACGAG	1 cut(s)	2547
Bst2UI	CCWGG	5 cut(s)	258, 712, 999, 1185, 1218
Bst4CI	ACNGT	2 cut(s)	2162, 2257
BstACI	GRCGYC	1 cut(s)	1989
BstAFI	CTTAAG	2 cut(s)	1079, 1904
BstAUI	TGTACA	1 cut(s)	1793
BstC8I	GCNNGC	3 cut(s)	1290, 1356, 1563
BstDSI	CCRYGG	1 cut(s)	2449
BstENI	CCTNNNNNAGG	1 cut(s)	2126
BstF5I	GGATG	1 cut(s)	2571
BstFNI	CGCG	4 cut(s)	484, 1605, 2499, 2673
BstH2I	RGCGCY	1 cut(s)	2351
BstHHI	GCGC	4 cut(s)	193, 486, 2350, 2499
BstMAI	GTCTC	2 cut(s)	2286, 2662
BstMCI	CGRYCG	2 cut(s)	1163, 1277
BstMWI	GCNNNNNNNGC	3 cut(s)	202, 1285, 1571
BstNI	CCWGG	5 cut(s)	258, 712, 999, 1185, 1218
BstNSI	RCATGY	1 cut(s)	2213
BstSCI	CCNGG	6 cut(s)	256, 424, 710, 997, 1183, 1216
BstSFI	CTRYAG	2 cut(s)	663, 1623
BstSLI	GKGCMC	1 cut(s)	1262
BstUI	CGCG	4 cut(s)	484, 1605, 2499, 2673
BstV1I	GCAGC	6 cut(s)	205, 673, 692, 1460, 1463, 1483
BstV2I	GAAGAC	1 cut(s)	2560
BstX2I	RGATCY	6 cut(s)	542, 1400, 1518, 1715, 2116, 2632
BstXI	CCANNNNNNTGG	2 cut(s)	370, 1341
BstYI	RGATCY	6 cut(s)	542, 1400, 1518, 1715, 2116, 2632
BsuI	GTATCC	2 cut(s)	338, 1096
BsuRI	GGCC	8 cut(s)	143, 478, 757, 945, 1198, 1260, 1288, 2570
BtgI	CCRYGG	1 cut(s)	2449
BtrI	CACGTC	1 cut(s)	1235
BtsCI	GGATG	1 cut(s)	2571
BtsI	GCAGTG	1 cut(s)	2203
BtsIMutI	CAGTG	1 cut(s)	2203
BtuMI	TCGCGA	1 cut(s)	1605
BveI	ACCTGC	2 cut(s)	148, 616
Cac8I	GCNNGC	3 cut(s)	1290, 1356, 1563
CaiI	CAGNNNCTG	4 cut(s)	1013, 1642, 2255, 2647
CciI	TCATGA	1 cut(s)	628
CfoI	GCGC	4 cut(s)	193, 486, 2350, 2499
CseI	GACGC	2 cut(s)	1757, 2679
CsiI	ACCWGGT	1 cut(s)	1183
Csp6I	GTAC	5 cut(s)	1016, 1794, 1932, 2073, 2433
CspCI	CAANNNNNGTGG	2 cut(s)	793, 828
CviAII	CATG	8 cut(s)	139, 186, 629, 1802, 2210, 2273, 2386, 2450
CviQI	GTAC	5 cut(s)	1016, 1794, 1932, 2073, 2433
EaeI	YGGCCR	1 cut(s)	2568
EciI	GGCGGA	2 cut(s)	1167, 1227
Ecl136II	GAGCTC	1 cut(s)	376
Eco130I	CCWWGG	3 cut(s)	868, 2449, 2636
Eco147I	AGGCCT	2 cut(s)	757, 945
Eco24I	GRGCYC	2 cut(s)	378, 1262
Eco32I	GATATC	1 cut(s)	1750
Eco47I	GGWCC	4 cut(s)	1151, 1220, 1229, 1482
Eco47III	AGCGCT	1 cut(s)	2349
Eco53kI	GAGCTC	1 cut(s)	376
Eco57I	CTGAAG	8 cut(s)	326, 894, 1349, 1602, 1904, 2073, 2160, 2394
Eco88I	CYCGRG	2 cut(s)	2289, 2396
EcoICRI	GAGCTC	1 cut(s)	376
EcoNI	CCTNNNNNAGG	1 cut(s)	2126
EcoO109I	RGGNCCY	2 cut(s)	476, 1482
EcoRI	GAATTC	2 cut(s)	239, 1628
EcoRII	CCWGG	5 cut(s)	256, 710, 997, 1183, 1216
EcoRV	GATATC	1 cut(s)	1750
EcoT14I	CCWWGG	3 cut(s)	868, 2449, 2636
EcoT22I	ATGCAT	1 cut(s)	1300
EcoT38I	GRGCYC	2 cut(s)	378, 1262
ErhI	CCWWGG	3 cut(s)	868, 2449, 2636
Esp3I	CGTCTC	1 cut(s)	2662
FaeI	CATG	8 cut(s)	142, 189, 632, 1805, 2213, 2276, 2389, 2453
FalI	AAGNNNNNCTT	2 cut(s)	994, 1026
FaqI	GGGAC	2 cut(s)	2266, 2453
FatI	CATG	8 cut(s)	138, 185, 628, 1801, 2209, 2272, 2385, 2449
FauI	CCCGC	1 cut(s)	461
FauNDI	CATATG	1 cut(s)	171
FbaI	TGATCA	1 cut(s)	31
Fnu4HI	GCNGC	7 cut(s)	194, 687, 706, 1449, 1452, 1497, 2067
FokI	GGATG	1 cut(s)	2578
FriOI	GRGCYC	2 cut(s)	378, 1262
Fsp4HI	GCNGC	7 cut(s)	194, 687, 706, 1449, 1452, 1497, 2067
FspBI	CTAG	5 cut(s)	413, 1772, 2123, 2637, 2685
GlaI	GCGC	4 cut(s)	192, 485, 2349, 2498
GluI	GCNGC	7 cut(s)	194, 687, 706, 1449, 1452, 1497, 2067
GsuI	CTGGAG	3 cut(s)	392, 694, 1155
HaeII	RGCGCY	1 cut(s)	2351
HaeIII	GGCC	8 cut(s)	143, 478, 757, 945, 1198, 1260, 1288, 2570
HapII	CCGG	3 cut(s)	426, 1061, 1911
HgaI	GACGC	2 cut(s)	1757, 2679
HhaI	GCGC	4 cut(s)	193, 486, 2350, 2499
Hin1I	GRCGYC	1 cut(s)	1989
Hin1II	CATG	8 cut(s)	142, 189, 632, 1805, 2213, 2276, 2389, 2453
Hin6I	GCGC	4 cut(s)	191, 484, 2348, 2497
HinP1I	GCGC	4 cut(s)	191, 484, 2348, 2497
HincII	GTYRAC	1 cut(s)	2302
HindII	GTYRAC	1 cut(s)	2302
HindIII	AAGCTT	1 cut(s)	1953
HpaII	CCGG	3 cut(s)	426, 1061, 1911
HphI	GGTGA	5 cut(s)	41, 438, 597, 1709, 2437
Hpy166II	GTNNAC	3 cut(s)	1794, 1796, 2302
Hpy8I	GTNNAC	3 cut(s)	1794, 1796, 2302
HpyCH4III	ACNGT	2 cut(s)	2162, 2257
HpyCH4IV	ACGT	3 cut(s)	674, 1234, 1989
HpyCH4V	TGCA	9 cut(s)	596, 665, 689, 1298, 1354, 1448, 1598, 2225, 2529
HpyF10VI	GCNNNNNNNGC	3 cut(s)	202, 1285, 1571
HpySE526I	ACGT	3 cut(s)	674, 1234, 1989
Hsp92I	GRCGYC	1 cut(s)	1989
Hsp92II	CATG	8 cut(s)	142, 189, 632, 1805, 2213, 2276, 2389, 2453
HspAI	GCGC	4 cut(s)	191, 484, 2348, 2497
Ksp22I	TGATCA	1 cut(s)	31
LguI	GCTCTTC	1 cut(s)	436
LmnI	GCTCC	7 cut(s)	373, 713, 1048, 1111, 1180, 1318, 2022
Lsp1109I	GCAGC	6 cut(s)	205, 673, 692, 1460, 1463, 1483
LweI	GCATC	6 cut(s)	1285, 1300, 1585, 2224, 2391, 2606
MabI	ACCWGGT	1 cut(s)	1183
MaeI	CTAG	5 cut(s)	413, 1772, 2123, 2637, 2685
MaeII	ACGT	3 cut(s)	674, 1234, 1989
MaeIII	GTNAC	2 cut(s)	658, 896
MbiI	CCGCTC	1 cut(s)	2066
MflI	RGATCY	6 cut(s)	542, 1400, 1518, 1715, 2116, 2632
MhlI	GDGCHC	3 cut(s)	378, 1262, 1951
MlsI	TGGCCA	1 cut(s)	2570
MluI	ACGCGT	1 cut(s)	2671
MluNI	TGGCCA	1 cut(s)	2570
MlyI	GAGTC	6 cut(s)	52, 307, 1261, 1439, 1633, 2153
Mox20I	TGGCCA	1 cut(s)	2570
Mph1103I	ATGCAT	1 cut(s)	1300
MroXI	GAANNNNTTC	5 cut(s)	727, 1009, 1632, 1779, 1830
MscI	TGGCCA	1 cut(s)	2570
Msp20I	TGGCCA	1 cut(s)	2570
MspA1I	CMGCKG	1 cut(s)	1721
MspCI	CTTAAG	2 cut(s)	1079, 1904
MspI	CCGG	3 cut(s)	426, 1061, 1911
MspR9I	CCNGG	6 cut(s)	258, 426, 712, 999, 1185, 1218
Mva1269I	GAATGC	1 cut(s)	1576
MvaI	CCWGG	5 cut(s)	258, 712, 999, 1185, 1218
MvnI	CGCG	4 cut(s)	484, 1605, 2499, 2673
MwoI	GCNNNNNNNGC	3 cut(s)	202, 1285, 1571
NciI	CCSGG	1 cut(s)	426
NcoI	CCATGG	1 cut(s)	2449
NdeI	CATATG	1 cut(s)	171
NlaIII	CATG	8 cut(s)	142, 189, 632, 1805, 2213, 2276, 2389, 2453
NmuCI	GTSAC	1 cut(s)	658
NruI	TCGCGA	1 cut(s)	1605
NsiI	ATGCAT	1 cut(s)	1300
NspI	RCATGY	1 cut(s)	2213
PaeR7I	CTCGAG	2 cut(s)	2289, 2396
PagI	TCATGA	1 cut(s)	628
PaqCI	CACCTGC	1 cut(s)	616
PceI	AGGCCT	2 cut(s)	757, 945
PciI	ACATGT	1 cut(s)	2209
PciSI	GCTCTTC	1 cut(s)	436
PctI	GAATGC	1 cut(s)	1576
PdmI	GAANNNNTTC	5 cut(s)	727, 1009, 1632, 1779, 1830
PfeI	GAWTC	8 cut(s)	13, 64, 103, 209, 914, 1728, 1826, 2693
PflFI	GACNNNGTC	2 cut(s)	1233, 2297
PfoI	TCCNGGA	2 cut(s)	256, 1216
PkrI	GCNGC	7 cut(s)	195, 688, 707, 1450, 1453, 1498, 2068
PleI	GAGTC	6 cut(s)	51, 307, 1260, 1438, 1633, 2152
PpsI	GAGTC	6 cut(s)	51, 307, 1260, 1438, 1633, 2152
PpuMI	RGGWCCY	1 cut(s)	1482
PscI	ACATGT	1 cut(s)	2209
Psp124BI	GAGCTC	1 cut(s)	378
Psp5II	RGGWCCY	1 cut(s)	1482
Psp6I	CCWGG	5 cut(s)	256, 710, 997, 1183, 1216
PspGI	CCWGG	5 cut(s)	256, 710, 997, 1183, 1216
PspOMI	GGGCCC	1 cut(s)	1258
PspPPI	RGGWCCY	1 cut(s)	1482
PspXI	VCTCGAGB	1 cut(s)	2396
PstI	CTGCAG	1 cut(s)	667
PstNI	CAGNNNCTG	4 cut(s)	1013, 1642, 2255, 2647
PsuI	RGATCY	6 cut(s)	542, 1400, 1518, 1715, 2116, 2632
PsyI	GACNNNGTC	2 cut(s)	1233, 2297
RruI	TCGCGA	1 cut(s)	1605
RsaI	GTAC	5 cut(s)	1017, 1795, 1933, 2074, 2434
RsaNI	GTAC	5 cut(s)	1016, 1794, 1932, 2073, 2433
SacI	GAGCTC	1 cut(s)	378
SapI	GCTCTTC	1 cut(s)	436
SatI	GCNGC	7 cut(s)	194, 687, 706, 1449, 1452, 1497, 2067
SchI	GAGTC	6 cut(s)	52, 307, 1261, 1439, 1633, 2153
ScrFI	CCNGG	6 cut(s)	258, 426, 712, 999, 1185, 1218
SduI	GDGCHC	3 cut(s)	378, 1262, 1951
SexAI	ACCWGGT	1 cut(s)	1183
SfaNI	GCATC	6 cut(s)	1285, 1300, 1585, 2224, 2391, 2606
SfcI	CTRYAG	2 cut(s)	663, 1623
Sfr274I	CTCGAG	2 cut(s)	2289, 2396
SinI	GGWCC	4 cut(s)	1151, 1220, 1229, 1482
SlaI	CTCGAG	2 cut(s)	2289, 2396
SmlI	CTYRAG	8 cut(s)	1079, 1904, 1950, 2098, 2174, 2191, 2289, 2396
SmoI	CTYRAG	8 cut(s)	1079, 1904, 1950, 2098, 2174, 2191, 2289, 2396
SseBI	AGGCCT	2 cut(s)	757, 945
SspI	AATATT	1 cut(s)	856
SspMI	CTAG	5 cut(s)	413, 1772, 2123, 2637, 2685
SstI	GAGCTC	1 cut(s)	378
StuI	AGGCCT	2 cut(s)	757, 945
StyD4I	CCNGG	6 cut(s)	256, 424, 710, 997, 1183, 1216
StyI	CCWWGG	3 cut(s)	868, 2449, 2636
TaaI	ACNGT	2 cut(s)	2162, 2257
TaiI	ACGT	3 cut(s)	677, 1237, 1992
TaqI	TCGA	9 cut(s)	16, 496, 888, 1091, 1524, 2127, 2282, 2290, 2397
TaqII	GACCGA	1 cut(s)	808
TatI	WGTACW	3 cut(s)	1015, 1793, 2432
TauI	GCSGC	1 cut(s)	2069
TfiI	GAWTC	8 cut(s)	13, 64, 103, 209, 914, 1728, 1826, 2693
TscAI	CASTG	1 cut(s)	2203
TseFI	GTSAC	1 cut(s)	658
TseI	GCWGC	6 cut(s)	193, 686, 705, 1448, 1451, 1496
Tsp45I	GTSAC	1 cut(s)	658
TspDTI	ATGAA	9 cut(s)	127, 180, 765, 1431, 1621, 1818, 1937, 2327, 2616
TspRI	CASTG	1 cut(s)	2203
Tth111I	GACNNNGTC	2 cut(s)	1233, 2297
Vha464I	CTTAAG	2 cut(s)	1079, 1904
VpaK11BI	GGWCC	4 cut(s)	1151, 1220, 1229, 1482
XagI	CCTNNNNNAGG	1 cut(s)	2126
XapI	RAATTY	4 cut(s)	239, 583, 1073, 1628
XbaI	TCTAGA	1 cut(s)	1771
XceI	RCATGY	1 cut(s)	2213
XhoI	CTCGAG	2 cut(s)	2289, 2396
XmaJI	CCTAGG	1 cut(s)	2636
XmnI	GAANNNNTTC	5 cut(s)	727, 1009, 1632, 1779, 1830
XspI	CTAG	5 cut(s)	413, 1772, 2123, 2637, 2685
ZraI	GACGTC	1 cut(s)	1990
Zsp2I	ATGCAT	1 cut(s)	1300

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.