Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr11

Orthogroup ID

OG0004133

Physical Location & Seq

Genomic Coordinates Forward (+)

33203687 .. 33215969

Gene Structure 12283 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD11G1228300.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 3126 bp

ATGGGTCTTATGAAGACAAGTCGTTTTATTGTTTTTGTTATTCTGCTATGTGCTTCAGCATCAATGAGCTCAGAGGAGAGCAGAACCACAGAGGAAGTTTTTCTCAGTCAACTAGTTGATCCAGCAACTGGAAAGATTGATGGGGACATGGCACAGCTGTTATGGATTAGTTGCAGGGTAGATTTGATCCATATGACGGAAGCTATTGAACATCTTGACTTGTGGTTAGCAGAGGAAGCATCTAGTGGCACAGATGAAATTAATTCAAAAACACGGTTATTGGGAAAAGAAAAGTTTCAAAAGCTTATTAATGTCCTGCATCCTGAGGTGAAACATACTCTTTCAGATTGTTTAAGAAAACATAATCTCTTGTTCAGTGTGTCTGGAGAAGAGGGTGCCTCTAAGATTTGGTATGCTAAGTATATTGAGTCCCTATTTCCCAGACCTTATGTTCCAAGAAGAAATTTGGCTAGTGATTTGCCTCAGAGCATTCTTGATGTACCTTCTCCAGCCTTTGCACCAAGATATTCTCAACGTAGTCCAGCACCATCTCCTGGTCTAGCACCTTCCACTTCTCAAAGTCCTAGTCCTAGGCCTGCACGCCATTCTCCACAAGGATCATTTTTTCCTTCGGATCCCATAAATTCGATTCTTCAATCTTCAGGGCCAGCTTCCGGTTCAAATATACAGGAACATAAAGGACGAGATGAACATAAAACAATTGTTACTGCTGTTGTTATAACTGCATCAGTAACATTTATTGTTGCAGCATTGCTCTTTTTATGCTGTACTAAAATCTGTAGGAATGGGAAAAAGGGTAGACAAAATGATGAGAGGCCCCTCCTTAGCCTGAGTTTAACTGATTCTGCTGGTTCTTCATACAAGTCTTATGCTATGGGAAATTCAATGAAAGAAGAGAAGCTTGACCATCAATCATTAGAATTAGGATCCCCTGGAGGTGCTTCCAAGTTTGACGCGTCCAACAATATCAATGGACTAGTACCCCCTCCTCCTGGAATGTCTCCTCTGAAGCCCCCTCCTGGCAGAGCAAAACTTCTCCCACCTGAACCACCTTCTTCTTTTAGGCCTCCTCCCAGCAGGGCTAGTCCTCCTCCACCACCTGTACCTGCTGCTTTAAAAGCCGCAGGAAGTGCAGGCCCTCCCCCACCACCTCCTCCTGCACCACCTGGTCCTCGCCCCCCACCACCTTCTGGTCCTCATCCCCCACCACCTCCTGGCCCTCGCCCCCTACCACCTCCAAAGAGTGGCGTTCCTCCTCCTCGGCCACCTCCAGCAATGGCTATTGGTTCAAAGGTAGCTCGACCTCCACCTCTTGCACCAAAACGTCCACCGGATGCTGGATCGGAGGCTGATGGTGATGCTCCGAAAACCAAGCTGAAGCCCTTTTTTTGGGATAAGGTTCTAGCAAACCCGGATCACTCAATGGTTTGGCATCAGATTAAATCAGGATCGTTCCAGTTCGATGAAAATATGATTGAAACTCTCTTTGGGTATAACGCCGCTGATAAAAACAAAAATGAACTTAATAAAGAGTCTTCATCCCAAAATCCTACGCCCCAGCTCATTCAACTTATTAATCCCAAAAAGGCACAAAATCTATCAATTCTTTTGCGGGCATTGAATGTGACCATAGAAGAAGTCTGTGATGCAATTCGTGAAGGAAATGAGCTTCCATCAGAATTCTTAGAAACCTTGTTGAAGATGGCACCAACTCAAGAAGAAGAACTAAAGCTTAGACTGTTCAATGGTCCACTTTCTCAACTTGGGCCTGCTGAGCGGTTCCTGAAAGCCTTGATTGACATCCCATTTGCTTTTAAGCGATTGGAAGCATTACTTTTTATGTGCACTCTTCAGGAGGAGGCTACTCACCTTAAAGAGTCTTTCGAGACCTTAGAGGTTGCATGTAAGGAACTGAGAAGCAGCCGGCTTTTCCTCAAGCTTTTAGAAGCTGTTCTTAAAACTGGAAATCGCATGAATGACGGAACATTTCGTGGTGGAGCACAAGCATTCAAACTTGACACACTCTTGAAATTGTCAGATGTTAAAGGAATAGATGGCAAGACAACGCTTTTGCACTTTGTTGTTCAGGAAATAATCCGCTCTGAGGGTGTCAGAGCTGCTCGTACAGCAAAAGAGAGCAGGAGTTTCTCTAGCGTCAAAACAGATGATCTCCTTGAGGACACTTCCAACGAAACAGAAGAACAAGAACACTATCGCAGTCTTGGACTTGAGAAAGTTTCAGGTTTAAGCAATGAACTTGAGAATGTTAGAAAAGCATCTGTTTTGGATGCTGAAAGCTTAACAGGAACTGTTGCCAAACTTGGTCATTCGCTGATAAAGACCCGGGACTTCCTGAACACGGACATGAAAGATTCAGGGGAAGATAGTGAGTTCCATGAAACGCTGAAGAGTTTTGTGCAGAATGCTGAGGTTGATATCACGGGTCTCCTCGAGGAAGAAAAGAGAATCATGGCTCTGGTGAAGAGCACCGGTGACTACTTTCATGGGAATGCAGGGAAGGATGAAGGCTTACGACTGTTTATTATTGTCCGAGATTTTCTAATAATGCTAGATAAGGTTTGTAGAGAGGTAAGATTGACACCAAAAAAGTCGACAAAAGTTCAGAAAAAAGAGACATCTTCCTCAGATCCCCGCCAGCCTTCAACAATGCCATCTGCCTCCGATCTTCGGCAGCCTCCTTCTCCTGATCTTCATAAGCGGCTTTTCCCGGCAATCCAAGATCGACGAATGGATAATTCTAGTTCAGATGATGAAAGTGACCATCACTGTACAATCTCGACAACAGATGCTTTCGTGGTTCTTCTCAAGATGTTATCCATATCCACTGATCACAGAAGGATTAGTGAAAGGGAGTTAGAGTTGAGCCTGCGGACTATGCCCTTCTCTTCGCAGTGGAATTTCACTGGATTGAACCTACGGTCGCTTTGGAAGGTACCTCCGGTGATGTTTGACACCTACTCATGGCTGGCTGTGTACATCAATGACTGCTCACTGATCAGGCGGACGACAGGACTAGAGTCGCAGAACACCAATACTATACAGAACTGTGGCTGGTCATCATTCAAAAGGCGAACAATTTCTTGA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0007010 GO:0007015 GO:0007275 GO:0008064 GO:0008092 GO:0008150 GO:0008154 GO:0009524 GO:0009653 GO:0009791 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010638 GO:0016043 GO:0022414 GO:0022607 GO:0030029 GO:0030036 GO:0030041 GO:0030312 GO:0030832 GO:0030833 GO:0030838 GO:0031334 GO:0032271 GO:0032273 GO:0032501 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0033043 GO:0034622 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044424 GO:0044444 GO:0044464 GO:0045010 GO:0048316 GO:0048317 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051258 GO:0051493 GO:0051495 GO:0061458 GO:0065003 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0090066 GO:0090698 GO:0097435 GO:0110053 GO:1902903 GO:1902905

KEGG Pathways

Metabolic & Signaling

ko04320 map04320

Pfam Domains

Protein Families

FH2

Protein Analysis

1042

Amino Acids

114.69

Weight (kDa)

8.07

Isoelectric Point (pI)

61.05

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FH2	PF02181	460 - 864	3.5e-107	Formin Homology 2 Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

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Orthologous Genes (Group: OG0004133)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54650 AT5G54650
fragaria_vesca	FvH4_3g21670 FvH4_3g21670 FvH4_3g21670 FvH4_3g21670 FvH4_3g21670 FvH4_3g21670
malus_domestica	MD03G1213400.v1.1 MD11G1228300.v1.1
prunus_persica	Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1
pyrus_communis	pycom03g16200 pycom11g20090
rosa_chinensis	RchiOBHm_Chr5g0037151
rosa_laevigata	RLG00000033762
rosa_multiflora	Rmu_ssc0000448.1_g000003
rosa_roxburghii	Rroxscaffold_1G00043830
rosa_rugosa	Rorug05G0161100 Rorug05G0161100 Rorug05G0161200
rosa_samantha	Rh5AG252800 Rh5BG254700 Rh5CG287200 Rh5DG263200 Rh6CG204900
rosa_wichuraiana	Rw5G023330

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AanI	TTATAA	1 cut(s)	740
AbsI	CCTCGAGG	1 cut(s)	2471
Acc36I	ACCTGC	1 cut(s)	1135
Acc65I	GGTACC	1 cut(s)	2974
AccB1I	GGYRCC	3 cut(s)	395, 1726, 2974
AccB7I	CCANNNNNTGG	5 cut(s)	128, 554, 1211, 1235, 1265
AccBSI	CCGCTC	2 cut(s)	1798, 2121
AccI	GTMKAC	2 cut(s)	820, 2633
AccII	CGCG	1 cut(s)	977
AciI	CCGC	9 cut(s)	1143, 1521, 1633, 1798, 2119, 2674, 2740, 2911, 3043
AcoI	YGGCCR	1 cut(s)	1283
AcsI	RAATTY	5 cut(s)	463, 643, 901, 1700, 2938
AcuI	CTGAAG	6 cut(s)	39, 645, 1049, 1418, 1856, 2447
AfaI	GTAC	8 cut(s)	501, 790, 1002, 1125, 2146, 2812, 2976, 3017
AflIII	ACRYGT	1 cut(s)	975
AgeI	ACCGGT	1 cut(s)	2510
AhlI	ACTAGT	2 cut(s)	112, 997
AjnI	CCWGG	6 cut(s)	553, 952, 1012, 1039, 1186, 1234
AjuI	GAANNNNNNNTTGG	2 cut(s)	1491, 1523
Alw21I	GWGCWC	4 cut(s)	71, 1868, 2023, 2510
Alw26I	GTCTC	4 cut(s)	1026, 1901, 2471, 2648
Alw44I	GTGCAC	1 cut(s)	1864
AlwNI	CAGNNNCTG	2 cut(s)	128, 2330
Ama87I	CYCGRG	2 cut(s)	2364, 2471
AoxI	GGCC	8 cut(s)	593, 665, 836, 1085, 1156, 1237, 1283, 1787
ApaLI	GTGCAC	1 cut(s)	1864
ApeKI	GCWGC	5 cut(s)	767, 1130, 1941, 2138, 2713
ApoI	RAATTY	5 cut(s)	463, 643, 901, 1700, 2938
ArsI	GACNNNNNNTTYG	6 cut(s)	571, 603, 1811, 1843, 2074, 2106
AseI	ATTAAT	3 cut(s)	261, 309, 1596
AsiGI	ACCGGT	1 cut(s)	2510
Asp700I	GAANNNNTTC	4 cut(s)	99, 294, 1971, 3118
Asp718I	GGTACC	1 cut(s)	2974
AspA2I	CCTAGG	1 cut(s)	590
AspS9I	GGNCC	8 cut(s)	665, 837, 1157, 1190, 1214, 1238, 1769, 1787
AsuC2I	CCSGG	4 cut(s)	1433, 2365, 2366, 2750
AsuHPI	GGTGA	6 cut(s)	340, 1388, 1880, 2512, 2525, 2995
AvaI	CYCGRG	2 cut(s)	2364, 2471
AvaII	GGWCC	3 cut(s)	1190, 1214, 1769
AvrII	CCTAGG	1 cut(s)	590
AxyI	CCTNAGG	1 cut(s)	324
BaeGI	GKGCMC	1 cut(s)	1868
BamHI	GGATCC	2 cut(s)	634, 947
BanI	GGYRCC	3 cut(s)	395, 1726, 2974
BanII	GRGCYC	1 cut(s)	71
BbsI	GAAGAC	2 cut(s)	20, 1548
Bbv12I	GWGCWC	4 cut(s)	71, 1868, 2023, 2510
BbvCI	CCTCAGC	1 cut(s)	2448
BbvI	GCAGC	5 cut(s)	779, 1117, 1953, 2125, 2725
BccI	CCATC	9 cut(s)	134, 556, 936, 1367, 1702, 1717, 2069, 2701, 2811
BciT130I	CCWGG	6 cut(s)	555, 954, 1014, 1041, 1188, 1236
BclI	TGATCA	2 cut(s)	2869, 3036
BcnI	CCSGG	4 cut(s)	1433, 2365, 2366, 2750
BcoDI	GTCTC	4 cut(s)	1026, 1901, 2471, 2648
BcuI	ACTAGT	2 cut(s)	112, 997
BfmI	CTRYAG	1 cut(s)	799
BfuAI	ACCTGC	1 cut(s)	1135
BisI	GCNGC	8 cut(s)	768, 1131, 1143, 1521, 1942, 2139, 2714, 2741
BlnI	CCTAGG	1 cut(s)	590
BlpI	GCTNAGC	1 cut(s)	1794
BlsI	GCNGC	8 cut(s)	769, 1132, 1144, 1522, 1943, 2140, 2715, 2742
Bme18I	GGWCC	3 cut(s)	1190, 1214, 1769
BmeT110I	CYCGRG	2 cut(s)	2364, 2471
BmgT120I	GGNCC	8 cut(s)	665, 837, 1157, 1190, 1214, 1238, 1769, 1787
BmiI	GGNNCC	7 cut(s)	397, 636, 839, 949, 1728, 1802, 2976
BoxI	GACNNNNGTC	1 cut(s)	3058
BpiI	GAAGAC	2 cut(s)	20, 1548
BplI	GAGNNNNNCTC	2 cut(s)	383, 415
BpmI	CTGGAG	4 cut(s)	405, 492, 975, 1275
Bpu10I	CCTNAGC	2 cut(s)	845, 2448
Bpu1102I	GCTNAGC	1 cut(s)	1794
BpuEI	CTTGAG	6 cut(s)	1719, 1940, 2216, 2270, 2300, 2831
BpuMI	CCSGG	4 cut(s)	1433, 2365, 2366, 2750
BsaBI	GATNNNNATC	1 cut(s)	1820
BsaI	GGTCTC	2 cut(s)	1901, 2471
BsaJI	CCNNGG	4 cut(s)	590, 952, 1280, 2364
BsaWI	WCCGGW	4 cut(s)	674, 1351, 2510, 2980
BsaXI	ACNNNNNCTCC	2 cut(s)	2885, 2915
Bse118I	RCCGGY	2 cut(s)	1944, 2510
Bse1I	ACTGG	4 cut(s)	133, 1477, 1987, 2950
Bse21I	CCTNAGG	1 cut(s)	324
Bse3DI	GCAATG	3 cut(s)	770, 1302, 2278
Bse8I	GATNNNNATC	1 cut(s)	1820
BseBI	CCWGG	6 cut(s)	555, 954, 1014, 1041, 1188, 1236
BseDI	CCNNGG	4 cut(s)	590, 952, 1280, 2364
BseGI	GGATG	7 cut(s)	319, 1219, 1360, 1559, 1821, 2314, 2548
BseJI	GATNNNNATC	1 cut(s)	1820
BseMI	GCAATG	3 cut(s)	770, 1302, 2278
BseNI	ACTGG	4 cut(s)	133, 1477, 1987, 2950
BseSI	GKGCMC	1 cut(s)	1868
BseXI	GCAGC	5 cut(s)	779, 1117, 1953, 2125, 2725
BseYI	CCCAGC	2 cut(s)	1094, 1578
BsgI	GTGCAG	4 cut(s)	582, 1164, 1173, 2459
Bsh1236I	CGCG	1 cut(s)	977
Bsh1285I	CGRYCG	1 cut(s)	2963
BshFI	GGCC	8 cut(s)	595, 667, 838, 1087, 1158, 1239, 1285, 1789
BshNI	GGYRCC	3 cut(s)	395, 1726, 2974
BshTI	ACCGGT	1 cut(s)	2510
BsiEI	CGRYCG	1 cut(s)	2963
BsiHKAI	GWGCWC	4 cut(s)	71, 1868, 2023, 2510
BsiHKCI	CYCGRG	2 cut(s)	2364, 2471
BsiSI	CCGG	8 cut(s)	675, 1352, 1433, 1945, 2365, 2511, 2750, 2981
BslFI	GGGAC	3 cut(s)	158, 415, 2381
BsmAI	GTCTC	4 cut(s)	1026, 1901, 2471, 2648
BsmFI	GGGAC	3 cut(s)	158, 415, 2381
BsmI	GAATGC	4 cut(s)	489, 2027, 2449, 2536
BsnI	GGCC	8 cut(s)	595, 667, 838, 1087, 1158, 1239, 1285, 1789
Bso31I	GGTCTC	2 cut(s)	1901, 2471
BsoBI	CYCGRG	2 cut(s)	2364, 2471
Bsp1286I	GDGCHC	4 cut(s)	71, 1868, 2023, 2510
Bsp1407I	TGTACA	2 cut(s)	2810, 3015
Bsp1720I	GCTNAGC	1 cut(s)	1794
BspACI	CCGC	9 cut(s)	1143, 1521, 1633, 1798, 2119, 2674, 2740, 2911, 3043
BspANI	GGCC	8 cut(s)	595, 667, 838, 1087, 1158, 1239, 1285, 1789
BspFNI	CGCG	1 cut(s)	977
BspLI	GGNNCC	7 cut(s)	397, 636, 839, 949, 1728, 1802, 2976
BspMI	ACCTGC	1 cut(s)	1135
BspQI	GCTCTTC	1 cut(s)	2498
BspT107I	GGYRCC	3 cut(s)	395, 1726, 2974
BspTNI	GGTCTC	2 cut(s)	1901, 2471
BsrBI	CCGCTC	2 cut(s)	1798, 2121
BsrDI	GCAATG	3 cut(s)	770, 1302, 2278
BsrFI	RCCGGY	2 cut(s)	1944, 2510
BsrGI	TGTACA	2 cut(s)	2810, 3015
BsrI	ACTGG	4 cut(s)	133, 1477, 1987, 2950
BssAI	RCCGGY	2 cut(s)	1944, 2510
BssECI	CCNNGG	4 cut(s)	590, 952, 1280, 2364
BssT1I	CCWWGG	1 cut(s)	590
Bst2UI	CCWGG	6 cut(s)	555, 954, 1014, 1041, 1188, 1236
Bst4CI	ACNGT	7 cut(s)	276, 1761, 2332, 2559, 2810, 2961, 3089
Bst6I	CTCTTC	6 cut(s)	384, 909, 1875, 2423, 2498, 2932
BstAPI	GCANNNNNTGC	1 cut(s)	1151
BstAUI	TGTACA	2 cut(s)	2810, 3015
BstF5I	GGATG	7 cut(s)	319, 1219, 1360, 1559, 1821, 2314, 2548
BstFNI	CGCG	1 cut(s)	977
BstMAI	GTCTC	4 cut(s)	1026, 1901, 2471, 2648
BstMCI	CGRYCG	1 cut(s)	2963
BstMWI	GCNNNNNNNGC	6 cut(s)	236, 1139, 1151, 1795, 2147, 2917
BstNI	CCWGG	6 cut(s)	555, 954, 1014, 1041, 1188, 1236
BstNSI	RCATGY	1 cut(s)	1926
BstPAI	GACNNNNGTC	1 cut(s)	3058
BstSFI	CTRYAG	1 cut(s)	799
BstSLI	GKGCMC	1 cut(s)	1868
BstUI	CGCG	1 cut(s)	977
BstV1I	GCAGC	5 cut(s)	779, 1117, 1953, 2125, 2725
BstV2I	GAAGAC	2 cut(s)	20, 1548
BstX2I	RGATCY	3 cut(s)	634, 947, 2668
BstYI	RGATCY	3 cut(s)	634, 947, 2668
Bsu36I	CCTNAGG	1 cut(s)	324
BsuRI	GGCC	8 cut(s)	595, 667, 838, 1087, 1158, 1239, 1285, 1789
BtsCI	GGATG	7 cut(s)	319, 1219, 1360, 1559, 1821, 2314, 2548
BtsI	GCAGTG	1 cut(s)	2939
BtsIMutI	CAGTG	6 cut(s)	382, 2806, 2865, 2939, 2943, 3032
BveI	ACCTGC	1 cut(s)	1135
CaiI	CAGNNNCTG	2 cut(s)	128, 2330
Cfr10I	RCCGGY	2 cut(s)	1944, 2510
Cfr13I	GGNCC	8 cut(s)	665, 837, 1157, 1190, 1214, 1238, 1769, 1787
Cfr9I	CCCGGG	1 cut(s)	2364
CseI	GACGC	3 cut(s)	966, 983, 2164
CsiI	ACCWGGT	1 cut(s)	1186
Csp6I	GTAC	8 cut(s)	500, 789, 1001, 1124, 2145, 2811, 2975, 3016
CspAI	ACCGGT	1 cut(s)	2510
CviAII	CATG	8 cut(s)	148, 1923, 1993, 2386, 2417, 2491, 2525, 3003
CviQI	GTAC	8 cut(s)	500, 789, 1001, 1124, 2145, 2811, 2975, 3016
DraI	TTTAAA	1 cut(s)	1137
EaeI	YGGCCR	1 cut(s)	1283
Eam1104I	CTCTTC	6 cut(s)	384, 909, 1875, 2423, 2498, 2932
EarI	CTCTTC	6 cut(s)	384, 909, 1875, 2423, 2498, 2932
EciI	GGCGGA	1 cut(s)	3058
Ecl136II	GAGCTC	1 cut(s)	69
Eco130I	CCWWGG	1 cut(s)	590
Eco147I	AGGCCT	2 cut(s)	595, 1087
Eco24I	GRGCYC	1 cut(s)	71
Eco31I	GGTCTC	2 cut(s)	1901, 2471
Eco32I	GATATC	1 cut(s)	2458
Eco47I	GGWCC	3 cut(s)	1190, 1214, 1769
Eco53kI	GAGCTC	1 cut(s)	69
Eco57I	CTGAAG	6 cut(s)	39, 645, 1049, 1418, 1856, 2447
Eco81I	CCTNAGG	1 cut(s)	324
Eco88I	CYCGRG	2 cut(s)	2364, 2471
EcoICRI	GAGCTC	1 cut(s)	69
EcoO109I	RGGNCCY	2 cut(s)	837, 1157
EcoRI	GAATTC	1 cut(s)	1700
EcoRII	CCWGG	6 cut(s)	553, 952, 1012, 1039, 1186, 1234
EcoRV	GATATC	1 cut(s)	2458
EcoT14I	CCWWGG	1 cut(s)	590
EcoT38I	GRGCYC	1 cut(s)	71
ErhI	CCWWGG	1 cut(s)	590
FaeI	CATG	8 cut(s)	151, 1926, 1996, 2389, 2420, 2494, 2528, 3006
FalI	AAGNNNNNCTT	6 cut(s)	906, 938, 1839, 1871, 1959, 1991
FaqI	GGGAC	3 cut(s)	158, 415, 2381
FatI	CATG	8 cut(s)	147, 1922, 1992, 2385, 2416, 2490, 2524, 3002
FauI	CCCGC	2 cut(s)	1626, 2681
FauNDI	CATATG	1 cut(s)	192
FbaI	TGATCA	2 cut(s)	2869, 3036
FblI	GTMKAC	2 cut(s)	820, 2633
Fnu4HI	GCNGC	8 cut(s)	768, 1131, 1143, 1521, 1942, 2139, 2714, 2741
FokI	GGATG	7 cut(s)	306, 1206, 1367, 1546, 1808, 2321, 2555
FriOI	GRGCYC	1 cut(s)	71
Fsp4HI	GCNGC	8 cut(s)	768, 1131, 1143, 1521, 1942, 2139, 2714, 2741
GluI	GCNGC	8 cut(s)	768, 1131, 1143, 1521, 1942, 2139, 2714, 2741
GsaI	CCCAGC	2 cut(s)	1098, 1582
GsuI	CTGGAG	4 cut(s)	405, 492, 975, 1275
HaeIII	GGCC	8 cut(s)	595, 667, 838, 1087, 1158, 1239, 1285, 1789
HapII	CCGG	8 cut(s)	675, 1352, 1433, 1945, 2365, 2511, 2750, 2981
HgaI	GACGC	3 cut(s)	966, 983, 2164
Hin1II	CATG	8 cut(s)	151, 1926, 1996, 2389, 2420, 2494, 2528, 3006
HincII	GTYRAC	2 cut(s)	110, 2634
HindII	GTYRAC	2 cut(s)	110, 2634
HindIII	AAGCTT	5 cut(s)	302, 920, 1751, 1958, 2317
HinfI	GANTC	8 cut(s)	428, 649, 863, 1553, 1898, 2393, 2487, 3059
HpaII	CCGG	8 cut(s)	675, 1352, 1433, 1945, 2365, 2511, 2750, 2981
HphI	GGTGA	6 cut(s)	340, 1388, 1880, 2512, 2525, 2995
Hpy166II	GTNNAC	7 cut(s)	110, 821, 1349, 1772, 1866, 2634, 3016
Hpy8I	GTNNAC	7 cut(s)	110, 821, 1349, 1772, 1866, 2634, 3016
Hpy99I	CGWCG	1 cut(s)	2769
HpyCH4III	ACNGT	7 cut(s)	276, 1761, 2332, 2559, 2810, 2961, 3089
HpyCH4IV	ACGT	2 cut(s)	535, 1345
HpyF10VI	GCNNNNNNNGC	6 cut(s)	236, 1139, 1151, 1795, 2147, 2917
HpySE526I	ACGT	2 cut(s)	535, 1345
Hsp92II	CATG	8 cut(s)	151, 1926, 1996, 2389, 2420, 2494, 2528, 3006
KpnI	GGTACC	1 cut(s)	2978
KroI	GCCGGC	1 cut(s)	1944
KroNI	GCCGGC	1 cut(s)	1946
Ksp22I	TGATCA	2 cut(s)	2869, 3036
LguI	GCTCTTC	1 cut(s)	2498
LmnI	GCTCC	2 cut(s)	1387, 2018
Lsp1109I	GCAGC	5 cut(s)	779, 1117, 1953, 2125, 2725
MabI	ACCWGGT	1 cut(s)	1186
MaeII	ACGT	2 cut(s)	535, 1345
MaeIII	GTNAC	5 cut(s)	724, 751, 1645, 2513, 2798
MbiI	CCGCTC	2 cut(s)	1798, 2121
MfeI	CAATTG	1 cut(s)	720
MflI	RGATCY	3 cut(s)	634, 947, 2668
MhlI	GDGCHC	4 cut(s)	71, 1868, 2023, 2510
MluI	ACGCGT	1 cut(s)	975
MlyI	GAGTC	4 cut(s)	437, 1562, 1907, 3068
MmeI	TCCRAC	2 cut(s)	1005, 2232
MroNI	GCCGGC	1 cut(s)	1944
MroXI	GAANNNNTTC	4 cut(s)	99, 294, 1971, 3118
MslI	CAYNNNNRTG	2 cut(s)	2384, 2529
MspA1I	CMGCKG	2 cut(s)	157, 1523
MspI	CCGG	8 cut(s)	675, 1352, 1433, 1945, 2365, 2511, 2750, 2981
MunI	CAATTG	1 cut(s)	720
Mva1269I	GAATGC	4 cut(s)	489, 2027, 2449, 2536
MvaI	CCWGG	6 cut(s)	555, 954, 1014, 1041, 1188, 1236
MvnI	CGCG	1 cut(s)	977
MwoI	GCNNNNNNNGC	6 cut(s)	236, 1139, 1151, 1795, 2147, 2917
NaeI	GCCGGC	1 cut(s)	1946
NciI	CCSGG	4 cut(s)	1433, 2365, 2366, 2750
NdeI	CATATG	1 cut(s)	192
NgoMIV	GCCGGC	1 cut(s)	1944
NlaIII	CATG	8 cut(s)	151, 1926, 1996, 2389, 2420, 2494, 2528, 3006
NlaIV	GGNNCC	7 cut(s)	397, 636, 839, 949, 1728, 1802, 2976
NmeAIII	GCCGAG	1 cut(s)	1261
NmuCI	GTSAC	3 cut(s)	1645, 2513, 2798
NspI	RCATGY	1 cut(s)	1926
PaeR7I	CTCGAG	1 cut(s)	2471
PceI	AGGCCT	2 cut(s)	595, 1087
PciSI	GCTCTTC	1 cut(s)	2498
PctI	GAATGC	4 cut(s)	489, 2027, 2449, 2536
PdiI	GCCGGC	1 cut(s)	1946
PdmI	GAANNNNTTC	4 cut(s)	99, 294, 1971, 3118
PfeI	GAWTC	4 cut(s)	649, 863, 2393, 2487
PflMI	CCANNNNNTGG	5 cut(s)	128, 554, 1211, 1235, 1265
PfoI	TCCNGGA	1 cut(s)	1012
PinAI	ACCGGT	1 cut(s)	2510
PkrI	GCNGC	8 cut(s)	769, 1132, 1144, 1522, 1943, 2140, 2715, 2742
PleI	GAGTC	4 cut(s)	436, 1561, 1906, 3067
PpsI	GAGTC	4 cut(s)	436, 1561, 1906, 3067
PshAI	GACNNNNGTC	1 cut(s)	3058
PshBI	ATTAAT	3 cut(s)	261, 309, 1596
PsiI	TTATAA	1 cut(s)	740
Psp124BI	GAGCTC	1 cut(s)	71
Psp6I	CCWGG	6 cut(s)	553, 952, 1012, 1039, 1186, 1234
PspFI	CCCAGC	2 cut(s)	1094, 1578
PspGI	CCWGG	6 cut(s)	553, 952, 1012, 1039, 1186, 1234
PspN4I	GGNNCC	7 cut(s)	397, 636, 839, 949, 1728, 1802, 2976
PspPI	GGNCC	8 cut(s)	665, 837, 1157, 1190, 1214, 1238, 1769, 1787
PspXI	VCTCGAGB	1 cut(s)	2471
PstNI	CAGNNNCTG	2 cut(s)	128, 2330
PsuI	RGATCY	3 cut(s)	634, 947, 2668
PvuII	CAGCTG	1 cut(s)	157
RsaI	GTAC	8 cut(s)	501, 790, 1002, 1125, 2146, 2812, 2976, 3017
RsaNI	GTAC	8 cut(s)	500, 789, 1001, 1124, 2145, 2811, 2975, 3016
RseI	CAYNNNNRTG	2 cut(s)	2384, 2529
SacI	GAGCTC	1 cut(s)	71
SalI	GTCGAC	1 cut(s)	2632
SapI	GCTCTTC	1 cut(s)	2498
SatI	GCNGC	8 cut(s)	768, 1131, 1143, 1521, 1942, 2139, 2714, 2741
Sau96I	GGNCC	8 cut(s)	665, 837, 1157, 1190, 1214, 1238, 1769, 1787
SchI	GAGTC	4 cut(s)	437, 1562, 1907, 3068
SduI	GDGCHC	4 cut(s)	71, 1868, 2023, 2510
SexAI	ACCWGGT	1 cut(s)	1186
SfcI	CTRYAG	1 cut(s)	799
Sfr274I	CTCGAG	1 cut(s)	2471
SgrAI	CRCCGGYG	1 cut(s)	2510
SinI	GGWCC	3 cut(s)	1190, 1214, 1769
SlaI	CTCGAG	1 cut(s)	2471
SmaI	CCCGGG	1 cut(s)	2366
SmiMI	CAYNNNNRTG	2 cut(s)	2384, 2529
SmlI	CTYRAG	7 cut(s)	1734, 1955, 2195, 2249, 2279, 2471, 2846
SmoI	CTYRAG	7 cut(s)	1734, 1955, 2195, 2249, 2279, 2471, 2846
SpeI	ACTAGT	2 cut(s)	112, 997
SseBI	AGGCCT	2 cut(s)	595, 1087
SsiI	CCGC	9 cut(s)	1143, 1521, 1633, 1798, 2119, 2674, 2740, 2911, 3043
SstI	GAGCTC	1 cut(s)	71
StuI	AGGCCT	2 cut(s)	595, 1087
StyI	CCWWGG	1 cut(s)	590
TaaI	ACNGT	7 cut(s)	276, 1761, 2332, 2559, 2810, 2961, 3089
TaiI	ACGT	2 cut(s)	538, 1348
TaqI	TCGA	8 cut(s)	647, 1321, 1482, 1905, 2472, 2633, 2764, 2819
TatI	WGTACW	3 cut(s)	788, 2810, 3015
TauI	GCSGC	3 cut(s)	1145, 1523, 2743
TfiI	GAWTC	4 cut(s)	649, 863, 2393, 2487
TscAI	CASTG	6 cut(s)	382, 2813, 2872, 2939, 2950, 3039
TseFI	GTSAC	3 cut(s)	1645, 2513, 2798
TseI	GCWGC	5 cut(s)	767, 1130, 1941, 2138, 2713
Tsp45I	GTSAC	3 cut(s)	1645, 2513, 2798
TspGWI	ACGGA	3 cut(s)	212, 2016, 2396
TspMI	CCCGGG	1 cut(s)	2364
TspRI	CASTG	6 cut(s)	382, 2813, 2872, 2939, 2950, 3039
Van91I	CCANNNNNTGG	5 cut(s)	128, 554, 1211, 1235, 1265
VneI	GTGCAC	1 cut(s)	1864
VpaK11BI	GGWCC	3 cut(s)	1190, 1214, 1769
VspI	ATTAAT	3 cut(s)	261, 309, 1596
XapI	RAATTY	5 cut(s)	463, 643, 901, 1700, 2938
XceI	RCATGY	1 cut(s)	1926
XhoI	CTCGAG	1 cut(s)	2471
XmaI	CCCGGG	1 cut(s)	2364
XmaJI	CCTAGG	1 cut(s)	590
XmiI	GTMKAC	2 cut(s)	820, 2633
XmnI	GAANNNNTTC	4 cut(s)	99, 294, 1971, 3118

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.