Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5A

Orthogroup ID

OG0004133

Physical Location & Seq

Genomic Coordinates Reverse (-)

32459306 .. 32465841

Gene Structure 6536 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5AG252800.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2901 bp

ATGATAATACTACAACAAATGGGTCTTATGAGGACAAGTCGTTTCGTAGTTTTGGTTACTCTGCTTTGTGTTTCAGCTTTGATCAGCTCGGAGGAGAGAGTGATCACAGAAGAAGTGTTTCTCAGTAGACTAGTTGATCCAGCAACTGGAAAGATTGATGGAGATATGGCAGAGCTGTTATGGGTACGTTGCAAGGTAGATTTGACCCATTTGGTGGAAACTATTCAAGATCTTCACTTATGGTTACCAGAGGAAACATCTAGTAGCGCAGATGAAATTAGTTCAAAAAGACAGTCACTGGAAGAAGAAAATGTGCAGAAACTTATTAGTGTCCTGCATCCTCAGGTGAAACAAACTCTTTCAGATTGTTTCAGAAAGAATAATCTCTTTTTCTGTGTCTCTGGAGAAGAGAATGCCTCCAAGATTTGGTATGCCAAGTATATTGAGTCCCTATTTCCCAGACCTGTTCCAAGAAGGAATATGGGCAGTGAATTACTCCAGACCTCTGCTGAGGTACCTTCTCCTGCCCCTGCACCAGGATCTTCTAAACTCAGTCCAGCATCATCCCCTGGTCCTGCGCCATCTGCTTCTAGCATTTCTAGGTCCTCGGGCCCTACTACTCCACAAGGATCATTTTTTCCTTCTGTGTCCCGAAATTCGAGTTCACAACCTTCAGTTAGCGAACCAGCTCCCAGCACCATTATACAGATAACTAAGAAAAAGACTGACCATAAAACAATTATCATTGCAGTTGTTGTAACTGCAACAGTGACATTTGTTGTTGCAGCAGTCTTCTTTCTATGCTGTACTAAAATCTGTAGGACACGGCGTAAAGATAGAAAAAATGATGAGAGCCCCCTTCTTTGCCTGAGTAAATCCGACTCTGGAGGTTCTTCTTACAAGTCTTATCCTTTGGAAAATTCAATAAAAGGAGAGAAGATTGAGCATCAATCATTGGAATTAGGATCCATTGGACGTGCATCCAAATCCCGGTATGAAGTGTATGAGGCATCCAGCAATGGGAACGGATGGATACCACCTCCTCCTGGAAGGCCTCCTAATGGACCGCCTCCTCTGAAGCCCCCTCCTGGCAGGGCAAATCCTCTCCCTCCTGAGCCACCTTCTTCTTTTAAGCCTCCTCCTGGCAGGGCGGGTCCTCCTCCAACCCCTCCGCCTCCTGTACCACCTCCCACTTTGAAACCATCTGGTGGTGCAGCCCCTCCCCCACCACCTCCAGCTCCTGGTCCTCGGCCCCCACCACCTCCAGCTCCTGGTCCTCGCCCCCCGCCACCTTCAGCTCCTGGTCCTCCTCCCCCACCACTTCCAAAGATTGGTGTACCTCCTCCTAGGCCACCTCCATCAATGCCTATAGGTTCTAAGCTAGCTCGACCTCCACCATTTGTACCAAAGCGTCCATCAAATGCTGCTTCGAGTGATGGCTCAGAGGCTGATGGTGATGCTCCTAAAACCAAGCTGAAGCCCTTTTTTTGGGATAAGGTTCTAGCAAACCCTGATCATTCAATGGTGTGGCATCAGATTAAATCAGGATCTTTCCAGTTTGATGAGAACATGATTGAATCTCTGTTTGGGTATAATGCTGGTGATAAAAACAAAAACGATCGCAAGAAGGAGTCTTCATCCCAAGATCCTACACCCCAGTTCATTCAACTTATTAATCCCAAAAAGGCACAGAATCTATCAATTCTTCTCCGAGCTTTGAATGTGACAATAGAAGAAGTCTGCGATGCAATTTGTGAAGGAAATGAGCTTCCCATTGAATTCGTTCAAACTTTGTTGAAGATGGCACCAACACAAGAAGAAGAACTAAAGCTCAGGGTGTTCGATGGTGCACTTTCTCAACTTGGGCCTGCTGAACGGTTCTTAAAAGGCTTAATCGACATCCCTTTTGCCTTTAAACGGTTGGAAGCCTTACTTTTTATGTGCACTCTTCAGGAGGAGGTCACCAACCTTAAAGAATCCTTCGCAACCTTAGAGGTTGCTTGCAAGGAACTCAGAAACAGCCGGCTTTTCCTCAAGCTTTTAGAAGCTGTTCTTAAAACTGGCAATCGCATGAATGATGGAACATTTCGTGGTGGTGCACAGGCATTTAAGCTGGACACACTCTTGAAATTGTCAGATGTAAAGGGAACAGATGGCAAGACAACTCTTTTGCACTTTGTTGTTCAGGAAATAATCCGCTCTGAGGGTGTGAGAGCTGCTCGTGCAGCAAAAGAGAACCGGAGTTTCTCTAGCGTCAAAACAGATGATCTCCTTGAGGAAACTTCCCATGACACAGAAGAGCACTATTGTAATCTTGGTCTGGAGAAAGTATCAGGTTTAAGCAATGAACTCGAGAATGTAAAAAAAGCATCAATTTTGGATGCTGATAGCTTAACAGGAACTGTTGCCAAACTTGGAAGTGCTCTGGTAAAAGCCCGAGATTTTCTGAACTCAGACATGAAGAAGTCAGGGGAAGAAAGTGGTTTTCATGAAACACTGAAGAGTTTTGTGCAGAATGCTGATGTTGATATCAGGGGTCTCCGTGAGGAAGAAAAGAGAATCATGGCTCTGGTAAAGAGCACCGGAGACTATTTCCATGGGAATGCAGGGAAGGATGAGGGTTTACATTTGTTTGTTGTTGTGCGGGATTTTTTAATAATTTTAGATAAGGTATGCAAAGAAGTGAGATTGAATCCAAAAAAGTTCGCAAAGGTTCAGAAAAAGGAGGCACCTTCCTCTGATCCCCACCAGCCTCCTACAACGCCATCTGCCTCTGATCCCCACCAGCCTCCTTCTCCTGGTCTACGCCAGCCTCCTTTTCCAGATCTCCATCAGCGGCTTTTCCCGGCAATCAAGGATCGGCGCATGGACAATTCTAGCTCAGATGATGAAGGTCCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0007010 GO:0007015 GO:0007275 GO:0008064 GO:0008092 GO:0008150 GO:0008154 GO:0009524 GO:0009653 GO:0009791 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010638 GO:0016043 GO:0022414 GO:0022607 GO:0030029 GO:0030036 GO:0030041 GO:0030312 GO:0030832 GO:0030833 GO:0030838 GO:0031334 GO:0032271 GO:0032273 GO:0032501 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0033043 GO:0034622 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044424 GO:0044444 GO:0044464 GO:0045010 GO:0048316 GO:0048317 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051258 GO:0051493 GO:0051495 GO:0061458 GO:0065003 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0090066 GO:0090698 GO:0097435 GO:0110053 GO:1902903 GO:1902905

KEGG Pathways

Metabolic & Signaling

ko04320 map04320

Pfam Domains

Protein Families

FH2

Protein Analysis

966

Amino Acids

105.32

Weight (kDa)

8.52

Isoelectric Point (pI)

56.97

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FH2	PF02181	486 - 887	6.1e-110	Formin Homology 2 Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Image

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Orthologous Genes (Group: OG0004133)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54650 AT5G54650
fragaria_vesca	FvH4_3g21670 FvH4_3g21670 FvH4_3g21670 FvH4_3g21670 FvH4_3g21670 FvH4_3g21670
malus_domestica	MD03G1213400.v1.1 MD11G1228300.v1.1
prunus_persica	Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1
pyrus_communis	pycom03g16200 pycom11g20090
rosa_chinensis	RchiOBHm_Chr5g0037151
rosa_laevigata	RLG00000033762
rosa_multiflora	Rmu_ssc0000448.1_g000003
rosa_roxburghii	Rroxscaffold_1G00043830
rosa_rugosa	Rorug05G0161100 Rorug05G0161100 Rorug05G0161200
rosa_samantha	Rh5AG252800 Rh5BG254700 Rh5CG287200 Rh5DG263200 Rh6CG204900
rosa_wichuraiana	Rw5G023330

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	514
AccB1I	GGYRCC	3 cut(s)	514, 1804, 2728
AccB7I	CCANNNNNTGG	7 cut(s)	146, 214, 426, 1208, 1241, 1271, 1331
AccBSI	CCGCTC	1 cut(s)	2199
AccI	GTMKAC	2 cut(s)	127, 2803
AciI	CCGC	7 cut(s)	1067, 1151, 1172, 1286, 2197, 2644, 2836
AcsI	RAATTY	3 cut(s)	655, 919, 1778
AcuI	CTGAAG	6 cut(s)	657, 1097, 1278, 1496, 1934, 2519
AfaI	GTAC	6 cut(s)	186, 516, 808, 1182, 1338, 1404
AhlI	ACTAGT	1 cut(s)	130
AjiI	CACGTC	1 cut(s)	977
AjnI	CCWGG	9 cut(s)	535, 568, 1045, 1087, 1141, 1240, 1270, 1300, 2797
AjuI	GAANNNNNNNTTGG	2 cut(s)	1569, 1601
Alw21I	GWGCWC	6 cut(s)	1852, 1946, 2101, 2304, 2425, 2582
Alw26I	GTCTC	3 cut(s)	403, 2543, 2580
Alw44I	GTGCAC	3 cut(s)	1848, 1942, 2097
AlwNI	CAGNNNCTG	7 cut(s)	146, 298, 1241, 1271, 1301, 1448, 2402
Ama87I	CYCGRG	3 cut(s)	607, 2351, 2436
AoxI	GGCC	5 cut(s)	610, 1052, 1250, 1349, 1865
ApaI	GGGCCC	1 cut(s)	614
ApaLI	GTGCAC	3 cut(s)	1848, 1942, 2097
ApeKI	GCWGC	5 cut(s)	785, 1214, 1424, 2216, 2225
ApoI	RAATTY	3 cut(s)	655, 919, 1778
ArsI	GACNNNNNNTTYG	6 cut(s)	278, 310, 1889, 1921, 2152, 2184
AseI	ATTAAT	1 cut(s)	1674
Asp700I	GAANNNNTTC	4 cut(s)	117, 222, 1782, 2049
Asp718I	GGTACC	1 cut(s)	514
AspA2I	CCTAGG	1 cut(s)	1346
AspLEI	GCGC	3 cut(s)	269, 580, 2865
AsuC2I	CCSGG	2 cut(s)	991, 2846
AsuHPI	GGTGA	4 cut(s)	358, 1466, 1613, 1954
AsuNHI	GCTAGC	1 cut(s)	1381
AvaI	CYCGRG	3 cut(s)	607, 2351, 2436
AvaII	GGWCC	8 cut(s)	572, 603, 1064, 1154, 1244, 1274, 1304, 2894
AvrII	CCTAGG	1 cut(s)	1346
AxyI	CCTNAGG	1 cut(s)	342
BaeGI	GKGCMC	4 cut(s)	614, 1852, 1946, 2101
BamHI	GGATCC	1 cut(s)	965
BanI	GGYRCC	3 cut(s)	514, 1804, 2728
BanII	GRGCYC	2 cut(s)	614, 857
BauI	CACGAG	1 cut(s)	2220
BbsI	GAAGAC	2 cut(s)	784, 1626
Bbv12I	GWGCWC	6 cut(s)	1852, 1946, 2101, 2304, 2425, 2582
BbvCI	CCTCAGC	1 cut(s)	510
BbvI	GCAGC	5 cut(s)	797, 1226, 1411, 2203, 2237
BceAI	ACGGC	1 cut(s)	842
BcgI	CGANNNNNNTGC	2 cut(s)	2332, 2366
BciT130I	CCWGG	9 cut(s)	537, 570, 1047, 1089, 1143, 1242, 1272, 1302, 2799
BciVI	GTATCC	1 cut(s)	1026
BclI	TGATCA	3 cut(s)	81, 102, 1513
BcnI	CCSGG	2 cut(s)	991, 2846
BcoDI	GTCTC	3 cut(s)	403, 2543, 2580
BcuI	ACTAGT	1 cut(s)	130
BfaI	CTAG	9 cut(s)	131, 261, 591, 600, 1347, 1382, 1502, 2250, 2877
BfmI	CTRYAG	2 cut(s)	817, 1368
BfuI	GTATCC	1 cut(s)	1026
BglII	AGATCT	2 cut(s)	229, 2824
BisI	GCNGC	6 cut(s)	786, 1215, 1425, 2217, 2226, 2837
BlnI	CCTAGG	1 cut(s)	1346
BlsI	GCNGC	6 cut(s)	787, 1216, 1426, 2218, 2227, 2838
Bme18I	GGWCC	8 cut(s)	572, 603, 1064, 1154, 1244, 1274, 1304, 2894
BmeT110I	CYCGRG	3 cut(s)	607, 2351, 2436
BmgBI	CACGTC	1 cut(s)	977
BmiI	GGNNCC	7 cut(s)	516, 612, 967, 1155, 1253, 1806, 2730
BmrI	ACTGGG	1 cut(s)	1651
BmtI	GCTAGC	1 cut(s)	1385
BmuI	ACTGGG	1 cut(s)	1651
BpiI	GAAGAC	2 cut(s)	784, 1626
BplI	GAGNNNNNCTC	4 cut(s)	401, 433, 2203, 2235
BpmI	CTGGAG	6 cut(s)	423, 482, 906, 1218, 1248, 2342
Bpu10I	CCTNAGC	3 cut(s)	510, 1113, 1832
BpuEI	CTTGAG	2 cut(s)	2018, 2294
BpuMI	CCSGG	2 cut(s)	991, 2846
BsaBI	GATNNNNATC	1 cut(s)	2829
BsaI	GGTCTC	1 cut(s)	2543
BsaJI	CCNNGG	5 cut(s)	568, 606, 1247, 1346, 2596
BsaWI	WCCGGW	2 cut(s)	2238, 2582
Bse118I	RCCGGY	1 cut(s)	2022
Bse1I	ACTGG	5 cut(s)	151, 303, 1555, 1657, 2065
Bse21I	CCTNAGG	1 cut(s)	342
Bse3DI	GCAATG	3 cut(s)	744, 1024, 2350
Bse8I	GATNNNNATC	1 cut(s)	2829
BseBI	CCWGG	9 cut(s)	537, 570, 1047, 1089, 1143, 1242, 1272, 1302, 2799
BseDI	CCNNGG	5 cut(s)	568, 606, 1247, 1346, 2596
BseGI	GGATG	9 cut(s)	337, 563, 980, 1010, 1034, 1637, 1899, 2386, 2620
BseJI	GATNNNNATC	1 cut(s)	2829
BseMI	GCAATG	3 cut(s)	744, 1024, 2350
BseNI	ACTGG	5 cut(s)	151, 303, 1555, 1657, 2065
BseRI	GAGGAG	8 cut(s)	107, 1032, 1062, 1128, 1149, 1299, 1332, 1970
BseSI	GKGCMC	4 cut(s)	614, 1852, 1946, 2101
BseXI	GCAGC	5 cut(s)	797, 1226, 1411, 2203, 2237
BseYI	CCCAGC	1 cut(s)	692
BsgI	GTGCAG	5 cut(s)	335, 516, 1233, 2244, 2531
Bsh1285I	CGRYCG	1 cut(s)	1621
BshFI	GGCC	5 cut(s)	612, 1054, 1252, 1351, 1867
BshNI	GGYRCC	3 cut(s)	514, 1804, 2728
BsiEI	CGRYCG	1 cut(s)	1621
BsiHKAI	GWGCWC	6 cut(s)	1852, 1946, 2101, 2304, 2425, 2582
BsiHKCI	CYCGRG	3 cut(s)	607, 2351, 2436
BsiSI	CCGG	5 cut(s)	991, 2023, 2239, 2583, 2846
BslFI	GGGAC	2 cut(s)	433, 634
BsmAI	GTCTC	3 cut(s)	403, 2543, 2580
BsmFI	GGGAC	2 cut(s)	433, 634
BsmI	GAATGC	3 cut(s)	418, 2521, 2608
BsnI	GGCC	5 cut(s)	612, 1054, 1252, 1351, 1867
Bso31I	GGTCTC	1 cut(s)	2543
BsoBI	CYCGRG	3 cut(s)	607, 2351, 2436
Bsp120I	GGGCCC	1 cut(s)	610
Bsp1286I	GDGCHC	8 cut(s)	614, 857, 1852, 1946, 2101, 2304, 2425, 2582
Bsp19I	CCATGG	1 cut(s)	2596
BspACI	CCGC	7 cut(s)	1067, 1151, 1172, 1286, 2197, 2644, 2836
BspANI	GGCC	5 cut(s)	612, 1054, 1252, 1351, 1867
BspHI	TCATGA	1 cut(s)	2488
BspLI	GGNNCC	7 cut(s)	516, 612, 967, 1155, 1253, 1806, 2730
BspOI	GCTAGC	1 cut(s)	1385
BspQI	GCTCTTC	1 cut(s)	2292
BspT107I	GGYRCC	3 cut(s)	514, 1804, 2728
BspTNI	GGTCTC	1 cut(s)	2543
BsrBI	CCGCTC	1 cut(s)	2199
BsrDI	GCAATG	3 cut(s)	744, 1024, 2350
BsrFI	RCCGGY	1 cut(s)	2022
BsrI	ACTGG	5 cut(s)	151, 303, 1555, 1657, 2065
BssAI	RCCGGY	1 cut(s)	2022
BssECI	CCNNGG	5 cut(s)	568, 606, 1247, 1346, 2596
BssSI	CACGAG	1 cut(s)	2220
BssT1I	CCWWGG	2 cut(s)	1346, 2596
Bst2BI	CACGAG	1 cut(s)	2220
Bst2UI	CCWGG	9 cut(s)	537, 570, 1047, 1089, 1143, 1242, 1272, 1302, 2799
Bst4CI	ACNGT	5 cut(s)	294, 769, 1878, 1920, 2404
Bst6I	CTCTTC	4 cut(s)	402, 1953, 2292, 2495
BstC8I	GCNNGC	5 cut(s)	1383, 1869, 2002, 2024, 2810
BstDSI	CCRYGG	1 cut(s)	2596
BstEII	GGTNACC	2 cut(s)	243, 1960
BstENI	CCTNNNNNAGG	1 cut(s)	534
BstF5I	GGATG	9 cut(s)	337, 563, 980, 1010, 1034, 1637, 1899, 2386, 2620
BstHHI	GCGC	3 cut(s)	269, 580, 2865
BstMAI	GTCTC	3 cut(s)	403, 2543, 2580
BstMCI	CGRYCG	1 cut(s)	1621
BstMWI	GCNNNNNNNGC	3 cut(s)	584, 2222, 2225
BstNI	CCWGG	9 cut(s)	537, 570, 1047, 1089, 1143, 1242, 1272, 1302, 2799
BstPI	GGTNACC	2 cut(s)	243, 1960
BstSFI	CTRYAG	2 cut(s)	817, 1368
BstSLI	GKGCMC	4 cut(s)	614, 1852, 1946, 2101
BstV1I	GCAGC	5 cut(s)	797, 1226, 1411, 2203, 2237
BstV2I	GAAGAC	2 cut(s)	784, 1626
BstX2I	RGATCY	6 cut(s)	229, 539, 965, 1547, 1645, 2824
BstYI	RGATCY	6 cut(s)	229, 539, 965, 1547, 1645, 2824
Bsu36I	CCTNAGG	1 cut(s)	342
BsuI	GTATCC	1 cut(s)	1026
BsuRI	GGCC	5 cut(s)	612, 1054, 1252, 1351, 1867
BtgI	CCRYGG	1 cut(s)	2596
BtgZI	GCGATG	1 cut(s)	1758
BtrI	CACGTC	1 cut(s)	977
BtsCI	GGATG	9 cut(s)	337, 563, 980, 1010, 1034, 1637, 1899, 2386, 2620
BtsI	GCAGTG	1 cut(s)	493
BtsIMutI	CAGTG	4 cut(s)	296, 493, 774, 2495
Cac8I	GCNNGC	5 cut(s)	1383, 1869, 2002, 2024, 2810
CaiI	CAGNNNCTG	7 cut(s)	146, 298, 1241, 1271, 1301, 1448, 2402
CciI	TCATGA	1 cut(s)	2488
CfoI	GCGC	3 cut(s)	269, 580, 2865
Cfr10I	RCCGGY	1 cut(s)	2022
CseI	GACGC	2 cut(s)	1400, 2242
Csp6I	GTAC	6 cut(s)	185, 515, 807, 1181, 1337, 1403
CviAII	CATG	8 cut(s)	1570, 2071, 2288, 2458, 2489, 2563, 2597, 2866
CviQI	GTAC	6 cut(s)	185, 515, 807, 1181, 1337, 1403
DraI	TTTAAA	1 cut(s)	1915
Eam1104I	CTCTTC	4 cut(s)	402, 1953, 2292, 2495
EarI	CTCTTC	4 cut(s)	402, 1953, 2292, 2495
EciI	GGCGGA	1 cut(s)	1161
Eco130I	CCWWGG	2 cut(s)	1346, 2596
Eco147I	AGGCCT	1 cut(s)	1054
Eco24I	GRGCYC	2 cut(s)	614, 857
Eco31I	GGTCTC	1 cut(s)	2543
Eco32I	GATATC	1 cut(s)	2530
Eco47I	GGWCC	8 cut(s)	572, 603, 1064, 1154, 1244, 1274, 1304, 2894
Eco57I	CTGAAG	6 cut(s)	657, 1097, 1278, 1496, 1934, 2519
Eco81I	CCTNAGG	1 cut(s)	342
Eco88I	CYCGRG	3 cut(s)	607, 2351, 2436
Eco91I	GGTNACC	2 cut(s)	243, 1960
EcoNI	CCTNNNNNAGG	1 cut(s)	534
EcoO109I	RGGNCCY	3 cut(s)	603, 611, 1154
EcoO65I	GGTNACC	2 cut(s)	243, 1960
EcoRI	GAATTC	1 cut(s)	1778
EcoRII	CCWGG	9 cut(s)	535, 568, 1045, 1087, 1141, 1240, 1270, 1300, 2797
EcoRV	GATATC	1 cut(s)	2530
EcoT14I	CCWWGG	2 cut(s)	1346, 2596
EcoT38I	GRGCYC	2 cut(s)	614, 857
ErhI	CCWWGG	2 cut(s)	1346, 2596
FaeI	CATG	8 cut(s)	1573, 2074, 2291, 2461, 2492, 2566, 2600, 2869
FalI	AAGNNNNNCTT	4 cut(s)	1917, 1949, 2037, 2069
FaqI	GGGAC	2 cut(s)	433, 634
FatI	CATG	8 cut(s)	1569, 2070, 2287, 2457, 2488, 2562, 2596, 2865
FauI	CCCGC	3 cut(s)	1144, 1293, 2637
FbaI	TGATCA	3 cut(s)	81, 102, 1513
FblI	GTMKAC	2 cut(s)	127, 2803
Fnu4HI	GCNGC	6 cut(s)	786, 1215, 1425, 2217, 2226, 2837
FokI	GGATG	9 cut(s)	324, 550, 967, 997, 1041, 1624, 1886, 2393, 2627
FriOI	GRGCYC	2 cut(s)	614, 857
Fsp4HI	GCNGC	6 cut(s)	786, 1215, 1425, 2217, 2226, 2837
FspBI	CTAG	9 cut(s)	131, 261, 591, 600, 1347, 1382, 1502, 2250, 2877
GlaI	GCGC	3 cut(s)	268, 579, 2864
GluI	GCNGC	6 cut(s)	786, 1215, 1425, 2217, 2226, 2837
GsaI	CCCAGC	1 cut(s)	696
GsuI	CTGGAG	6 cut(s)	423, 482, 906, 1218, 1248, 2342
HaeIII	GGCC	5 cut(s)	612, 1054, 1252, 1351, 1867
HapII	CCGG	5 cut(s)	991, 2023, 2239, 2583, 2846
HgaI	GACGC	2 cut(s)	1400, 2242
HhaI	GCGC	3 cut(s)	269, 580, 2865
Hin1II	CATG	8 cut(s)	1573, 2074, 2291, 2461, 2492, 2566, 2600, 2869
Hin6I	GCGC	3 cut(s)	267, 578, 2863
HinP1I	GCGC	3 cut(s)	267, 578, 2863
HindIII	AAGCTT	1 cut(s)	2036
HinfI	GANTC	8 cut(s)	446, 881, 1577, 1631, 1693, 1976, 2559, 2692
HpaII	CCGG	5 cut(s)	991, 2023, 2239, 2583, 2846
HphI	GGTGA	4 cut(s)	358, 1466, 1613, 1954
Hpy166II	GTNNAC	8 cut(s)	128, 665, 1337, 1850, 1944, 2099, 2624, 2804
Hpy8I	GTNNAC	8 cut(s)	128, 665, 1337, 1850, 1944, 2099, 2624, 2804
HpyCH4III	ACNGT	5 cut(s)	294, 769, 1878, 1920, 2404
HpyCH4IV	ACGT	2 cut(s)	187, 976
HpyF10VI	GCNNNNNNNGC	3 cut(s)	584, 2222, 2225
HpySE526I	ACGT	2 cut(s)	187, 976
Hsp92II	CATG	8 cut(s)	1573, 2074, 2291, 2461, 2492, 2566, 2600, 2869
HspAI	GCGC	3 cut(s)	267, 578, 2863
KpnI	GGTACC	1 cut(s)	518
KroI	GCCGGC	1 cut(s)	2022
KroNI	GCCGGC	1 cut(s)	2024
Ksp22I	TGATCA	3 cut(s)	81, 102, 1513
LguI	GCTCTTC	1 cut(s)	2292
LmnI	GCTCC	5 cut(s)	694, 1243, 1273, 1303, 1465
Lsp1109I	GCAGC	5 cut(s)	797, 1226, 1411, 2203, 2237
MaeI	CTAG	9 cut(s)	131, 261, 591, 600, 1347, 1382, 1502, 2250, 2877
MaeII	ACGT	2 cut(s)	187, 976
MaeIII	GTNAC	7 cut(s)	55, 243, 294, 757, 769, 1723, 1960
MbiI	CCGCTC	1 cut(s)	2199
MflI	RGATCY	6 cut(s)	229, 539, 965, 1547, 1645, 2824
MhlI	GDGCHC	8 cut(s)	614, 857, 1852, 1946, 2101, 2304, 2425, 2582
MlyI	GAGTC	3 cut(s)	455, 875, 1640
MmeI	TCCRAC	3 cut(s)	903, 1187, 1902
MroNI	GCCGGC	1 cut(s)	2022
MroXI	GAANNNNTTC	4 cut(s)	117, 222, 1782, 2049
MslI	CAYNNNNRTG	1 cut(s)	2601
MspA1I	CMGCKG	1 cut(s)	2836
MspI	CCGG	5 cut(s)	991, 2023, 2239, 2583, 2846
Mva1269I	GAATGC	3 cut(s)	418, 2521, 2608
MvaI	CCWGG	9 cut(s)	537, 570, 1047, 1089, 1143, 1242, 1272, 1302, 2799
MwoI	GCNNNNNNNGC	3 cut(s)	584, 2222, 2225
NaeI	GCCGGC	1 cut(s)	2024
NciI	CCSGG	2 cut(s)	991, 2846
NcoI	CCATGG	1 cut(s)	2596
NgoMIV	GCCGGC	1 cut(s)	2022
NheI	GCTAGC	1 cut(s)	1381
NlaIII	CATG	8 cut(s)	1573, 2074, 2291, 2461, 2492, 2566, 2600, 2869
NlaIV	GGNNCC	7 cut(s)	516, 612, 967, 1155, 1253, 1806, 2730
NmeAIII	GCCGAG	1 cut(s)	1228
NmuCI	GTSAC	4 cut(s)	294, 769, 1723, 1960
PaeR7I	CTCGAG	1 cut(s)	2351
PagI	TCATGA	1 cut(s)	2488
PceI	AGGCCT	1 cut(s)	1054
PciSI	GCTCTTC	1 cut(s)	2292
PctI	GAATGC	3 cut(s)	418, 2521, 2608
PdiI	GCCGGC	1 cut(s)	2024
PdmI	GAANNNNTTC	4 cut(s)	117, 222, 1782, 2049
PfeI	GAWTC	5 cut(s)	1577, 1693, 1976, 2559, 2692
PflMI	CCANNNNNTGG	7 cut(s)	146, 214, 426, 1208, 1241, 1271, 1331
PfoI	TCCNGGA	1 cut(s)	1045
PkrI	GCNGC	6 cut(s)	787, 1216, 1426, 2218, 2227, 2838
Ple19I	CGATCG	1 cut(s)	1621
PleI	GAGTC	3 cut(s)	454, 875, 1639
PpsI	GAGTC	3 cut(s)	454, 875, 1639
PpuMI	RGGWCCY	2 cut(s)	603, 1154
PshBI	ATTAAT	1 cut(s)	1674
Psp5II	RGGWCCY	2 cut(s)	603, 1154
Psp6I	CCWGG	9 cut(s)	535, 568, 1045, 1087, 1141, 1240, 1270, 1300, 2797
PspEI	GGTNACC	2 cut(s)	243, 1960
PspFI	CCCAGC	1 cut(s)	692
PspGI	CCWGG	9 cut(s)	535, 568, 1045, 1087, 1141, 1240, 1270, 1300, 2797
PspN4I	GGNNCC	7 cut(s)	516, 612, 967, 1155, 1253, 1806, 2730
PspOMI	GGGCCC	1 cut(s)	610
PspPPI	RGGWCCY	2 cut(s)	603, 1154
PstNI	CAGNNNCTG	7 cut(s)	146, 298, 1241, 1271, 1301, 1448, 2402
PsuI	RGATCY	6 cut(s)	229, 539, 965, 1547, 1645, 2824
PvuI	CGATCG	1 cut(s)	1621
RsaI	GTAC	6 cut(s)	186, 516, 808, 1182, 1338, 1404
RsaNI	GTAC	6 cut(s)	185, 515, 807, 1181, 1337, 1403
RseI	CAYNNNNRTG	1 cut(s)	2601
SapI	GCTCTTC	1 cut(s)	2292
SatI	GCNGC	6 cut(s)	786, 1215, 1425, 2217, 2226, 2837
SchI	GAGTC	3 cut(s)	455, 875, 1640
SduI	GDGCHC	8 cut(s)	614, 857, 1852, 1946, 2101, 2304, 2425, 2582
SfcI	CTRYAG	2 cut(s)	817, 1368
Sfr274I	CTCGAG	1 cut(s)	2351
SinI	GGWCC	8 cut(s)	572, 603, 1064, 1154, 1244, 1274, 1304, 2894
SlaI	CTCGAG	1 cut(s)	2351
SmiMI	CAYNNNNRTG	1 cut(s)	2601
SmlI	CTYRAG	3 cut(s)	2033, 2273, 2351
SmoI	CTYRAG	3 cut(s)	2033, 2273, 2351
SpeI	ACTAGT	1 cut(s)	130
SseBI	AGGCCT	1 cut(s)	1054
SsiI	CCGC	7 cut(s)	1067, 1151, 1172, 1286, 2197, 2644, 2836
SspMI	CTAG	9 cut(s)	131, 261, 591, 600, 1347, 1382, 1502, 2250, 2877
StuI	AGGCCT	1 cut(s)	1054
StyI	CCWWGG	2 cut(s)	1346, 2596
TaaI	ACNGT	5 cut(s)	294, 769, 1878, 1920, 2404
TaiI	ACGT	2 cut(s)	190, 979
TaqI	TCGA	6 cut(s)	659, 1387, 1430, 1842, 1896, 2352
TatI	WGTACW	1 cut(s)	806
TauI	GCSGC	1 cut(s)	2839
TfiI	GAWTC	5 cut(s)	1577, 1693, 1976, 2559, 2692
TscAI	CASTG	4 cut(s)	303, 493, 774, 2502
TseFI	GTSAC	4 cut(s)	294, 769, 1723, 1960
TseI	GCWGC	5 cut(s)	785, 1214, 1424, 2216, 2225
Tsp45I	GTSAC	4 cut(s)	294, 769, 1723, 1960
TspDTI	ATGAA	9 cut(s)	288, 1011, 1626, 1651, 2087, 2361, 2474, 2477, 2505
TspGWI	ACGGA	2 cut(s)	1041, 2531
TspRI	CASTG	4 cut(s)	303, 493, 774, 2502
Van91I	CCANNNNNTGG	7 cut(s)	146, 214, 426, 1208, 1241, 1271, 1331
VneI	GTGCAC	3 cut(s)	1848, 1942, 2097
VpaK11BI	GGWCC	8 cut(s)	572, 603, 1064, 1154, 1244, 1274, 1304, 2894
VspI	ATTAAT	1 cut(s)	1674
XagI	CCTNNNNNAGG	1 cut(s)	534
XapI	RAATTY	3 cut(s)	655, 919, 1778
XhoI	CTCGAG	1 cut(s)	2351
XmaJI	CCTAGG	1 cut(s)	1346
XmiI	GTMKAC	2 cut(s)	127, 2803
XmnI	GAANNNNTTC	4 cut(s)	117, 222, 1782, 2049
XspI	CTAG	9 cut(s)	131, 261, 591, 600, 1347, 1382, 1502, 2250, 2877

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.