Basic Information

Type: gene

Biological Identity

Species

rosa_samantha

Chromosome

Chr5B

Orthogroup ID

OG0004133

Physical Location & Seq

Genomic Coordinates Reverse (-)

31655834 .. 31662447

Gene Structure 6614 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

Rh5BG254700.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2919 bp

ATGATAATACTACAACAAATGGGTCTTATGAGGACAAGTCGTTTCGTAGTTTTGGTTACTCTGCTTTGTGTTTCAGCTTTGATCAGCTCGGAGGAGAGAGTGATCACAGAAGAAGTGTTTCTCAGTAGACTAGTTGATCCAGCAACTGGAAAGATTGATGGAGATATGGCAGAGCTGTTATGGGTACGTTGCAAGGTAGATTTGACCCAGTTGGTGGAAACTATTCAAGATCTTCACTTATGGTTACCAGAGGAAACATCTAGTAGCGCAGATGAAATTAGTTCAAAAAGACAGTCACTGGAAGAAGAAAATGTGCAGAAACTTATTAGTGTCCTGCATCCTCAGGTGAAACAAACTCTTTCAGATTGTTTCAGAAAGAATAATCTCTTTTTCCGTGTCTCTGGAGAAGAGAATGCCTCCAAGATTTGGTATGCCAAGTATATTGAGTCCCTATTTCCCAGACCTGTTCCAAGAAGGAATATGGGCAGTGAATTGCTCCAGACCTCTGCTGAGGTACCTTCTCCTGCCCCTGCACCAGGATCTTCTAAACTCAGTCCAGCATCATCCCCTGGTCCTGCGCCATCTGCTTCTAGCATTTCTAGGTCCTCGGGCCCTACTACTCCACAAGGATCATTTTTTCCTGTGTCCCGAAATTCGAGTTCACAACCTTCAGTTAGCGAACCAGCTCCCAGCACCATTATACAGATAACTAAGAAAAAGACTGACCATAAAACAATTATCATTGCAGTTGTTGTAACTGCAACAGTGACATTTGTTGTTGCAGCAGTCTTCTTTCTATGCTGTACTAAAATCTGTAGGACACGGCGTAAAGATAGAAAAAATGATGAGAGCCCCCTTCTTTGCCTGAGTAAATCCGACTCTGGAGGTTCTTCTTACAAGTCTTATCCTTTGGAAAATTCAATAAAAGGAGAGAAGATTGAGCATCAATCATTGGAATTAGGATCCATTGGACGTGCATCCAAATCCCGGTATGAAGTGTATGAGGCATCCAGCAATGGGAACGGATGGATACCACCTCCTCCTGGAAGGCCTCCTAATGGACCGCCTCCTCTGAAGCCCCCTCCTGGCAGGGCAAATCCTCTCCCTCCTGAGCCACCTTCTTCTTTTAAGCCTCCTCCTGGCAGGGCGGGTCCTCCTCCTGGCAGGGCGGGTCCTCCTCCAACCCCTCCGCCTCCTGTACCACCTCCCACTTTGAAACCATCTGGTGGTGCAGCCCCTCCCCCACCACCTCCAGCTCCTGGTCCTCGGCCCCCACCACCTCCAGCTCCTGGTCCTCGGCCCCCGCCACCTTCATCTCCTGGTCCTCCTCCCCCACCACTTCCAAAGATTGGTGTACCTCCTCCTAGGCCACCTCCATCAATGCCTATAGGTTCTAAGCTAGCTCGACCTCCACCATTTGTATCAAAGCGTCCATCAAATGCTGCTTCGAGTGATGGCTCAGAGGCTGATGGTGATGCTCCTAAAACCAAGCTGAAGCCCTTTTTTTGGGATAAGGTTCTAGCAAACCCTGATCATTCAATGGTGTGGCATCAGATTAAATCAGGATCTTTCCAGTTTGATGAGAACATGATTGAATCTCTGTTTGGGTATAATGCTGGTGATAAAAACAAAAACGATCGCAAGAAGGAGTCTTCATCCCAAGATCCTACACCCCAGTTCATTCAACTTATTAATCCCAAAAAGGCACAGAATCTATCAATTCTTCTCCGAGCTTTGAATGTGACAATAGAAGAAGTCTGCGATGCAATTTGTGAAGGAAATGAGCTTCCCATTGAATTCGTTCAAACTTTGTTGAAGATGGCACCAACACAAGAAGAAGAACTAAAGCTCAGGGTGTTCAATGGTGCACTTTCTCAACTTGGGCCTGCTGAACGGTTCTTAAAAGGCTTAATCGACATCCCTTTTGCCTTTAAACGGTTGGAAGCGTTACTTTTTATGTGCACTCTTCAGGAGGAGGTCACCAACCTTAAAGAATCCTTCGCAACCTTAGAGGTTGCTTGCAAGGAACTCAGAAACAGCCGGCTTTTCCTCAAGCTTTTAGAAGCTGTTCTTAAAACTGGCAATCGCATGAATGATGGAACATTTCGTGGTGGTGCACAGGCATTTAAGCTGGACACACTCTTGAAATTGTCAGATGTAAAGGGAACAGATGGCAAGACAACTCTTTTGCACTTTGTTGTTCAGGAAATAATCCGCTCTGAGGGTGTGAGAGCTGCTCGTGCAGCAAAAGAGAACCGGAGTTTCTCTAGCGTCAAAACAGATGATCTCCTTGAGGAAACTTCCCATGACACAGAAGAGCACTATTGTAATCTTGGTCTGGAGAAAGTATCAGGTTTAAGCAATGAACTCGAGAATGTAAAAAAAGCATCAATTTTGGATGCTGATAGCTTAACAGGAACTGTTGCCAAACTTGGAAGTGCTCTGGTAAAAGCCCGAGATTTTCTGAACTCAGACATGAAGAATTCAGGGGAAGAAAGTGGTTTTCATGAAACACTGAAGAGTTTTGTGCAGAATGCTGATGTTGATATCAGGGGTCTCCGTGAGGAAGAAAAGAGAATCATGGCTCTGGTAAAGAGCACTGGAGACTATTTCCATGGGAATGCAGGGAAGGATGAGGGTTTACATTTGTTTGTTGTTGTGCGGGATTTTTTAATAATTTTAGATAAGGTATGCAAAGAAGTGAGATTGAATCCAAAAAAGTTCGCAAAGGTTCAGAAAAAGGAGGCACCTTCCTCTGATCCCCACCAGCCTCTTACAACGCCATCTGCCTCTGATCCCCACCAGCCTCCTTCTCCTGGTCTACGCCAGCCTCCTTTTCCAGATCTCCATCAGCGGCTTTTCCCGGCAATCAAGGATCGGCGCATGGACAATTCTAGCTCAGATGATGAAGGTCCATAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0000003 GO:0003006 GO:0003674 GO:0003779 GO:0005488 GO:0005515 GO:0005575 GO:0005618 GO:0005622 GO:0005623 GO:0005737 GO:0006996 GO:0007010 GO:0007015 GO:0007275 GO:0008064 GO:0008092 GO:0008150 GO:0008154 GO:0009524 GO:0009653 GO:0009791 GO:0009888 GO:0009960 GO:0009987 GO:0010154 GO:0010638 GO:0016043 GO:0022414 GO:0022607 GO:0030029 GO:0030036 GO:0030041 GO:0030312 GO:0030832 GO:0030833 GO:0030838 GO:0031334 GO:0032271 GO:0032273 GO:0032501 GO:0032502 GO:0032535 GO:0032956 GO:0032970 GO:0033043 GO:0034622 GO:0043254 GO:0043933 GO:0044085 GO:0044087 GO:0044089 GO:0044424 GO:0044444 GO:0044464 GO:0045010 GO:0048316 GO:0048317 GO:0048518 GO:0048522 GO:0048608 GO:0048731 GO:0048856 GO:0050789 GO:0050794 GO:0051128 GO:0051130 GO:0051258 GO:0051493 GO:0051495 GO:0061458 GO:0065003 GO:0065007 GO:0065008 GO:0071840 GO:0071944 GO:0090066 GO:0090698 GO:0097435 GO:0110053 GO:1902903 GO:1902905

KEGG Pathways

Metabolic & Signaling

ko04320 map04320

Pfam Domains

Protein Families

FH2

Protein Analysis

972

Amino Acids

105.92

Weight (kDa)

8.69

Isoelectric Point (pI)

56.85

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
FH2	PF02181	492 - 893	5.2e-110	Formin Homology 2 Domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

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Align Labels (Cladogram)

Image

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Orthologous Genes (Group: OG0004133)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G54650 AT5G54650
fragaria_vesca	FvH4_3g21670 FvH4_3g21670 FvH4_3g21670 FvH4_3g21670 FvH4_3g21670 FvH4_3g21670
malus_domestica	MD03G1213400.v1.1 MD11G1228300.v1.1
prunus_persica	Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1 Prupe.4G201000_v2.0.a1
pyrus_communis	pycom03g16200 pycom11g20090
rosa_chinensis	RchiOBHm_Chr5g0037151
rosa_laevigata	RLG00000033762
rosa_multiflora	Rmu_ssc0000448.1_g000003
rosa_roxburghii	Rroxscaffold_1G00043830
rosa_rugosa	Rorug05G0161100 Rorug05G0161100 Rorug05G0161200
rosa_samantha	Rh5AG252800 Rh5BG254700 Rh5CG287200 Rh5DG263200 Rh6CG204900
rosa_wichuraiana	Rw5G023330

Restriction Enzyme Sites

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Enzyme	Recognition Site	Cut Count	Positions (bp)
Acc65I	GGTACC	1 cut(s)	514
AccB1I	GGYRCC	3 cut(s)	514, 1822, 2746
AccB7I	CCANNNNNTGG	7 cut(s)	146, 214, 426, 1226, 1259, 1289, 1349
AccBSI	CCGCTC	1 cut(s)	2217
AccI	GTMKAC	2 cut(s)	127, 2821
AciI	CCGC	8 cut(s)	1064, 1148, 1169, 1190, 1304, 2215, 2662, 2854
AcsI	RAATTY	4 cut(s)	652, 916, 1796, 2482
AcuI	CTGAAG	5 cut(s)	654, 1094, 1514, 1952, 2537
AfaI	GTAC	5 cut(s)	186, 516, 805, 1200, 1356
AhlI	ACTAGT	1 cut(s)	130
AjiI	CACGTC	1 cut(s)	974
AjuI	GAANNNNNNNTTGG	2 cut(s)	1587, 1619
Alw21I	GWGCWC	6 cut(s)	1870, 1964, 2119, 2322, 2443, 2600
Alw26I	GTCTC	3 cut(s)	403, 2561, 2598
Alw44I	GTGCAC	3 cut(s)	1866, 1960, 2115
AlwNI	CAGNNNCTG	6 cut(s)	146, 298, 1259, 1289, 1466, 2420
Ama87I	CYCGRG	3 cut(s)	607, 2369, 2454
AoxI	GGCC	6 cut(s)	610, 1049, 1268, 1298, 1367, 1883
ApaI	GGGCCC	1 cut(s)	614
ApaLI	GTGCAC	3 cut(s)	1866, 1960, 2115
ApeKI	GCWGC	5 cut(s)	782, 1232, 1442, 2234, 2243
ApoI	RAATTY	4 cut(s)	652, 916, 1796, 2482
ArsI	GACNNNNNNTTYG	6 cut(s)	278, 310, 1907, 1939, 2170, 2202
AseI	ATTAAT	1 cut(s)	1692
Asp700I	GAANNNNTTC	4 cut(s)	117, 222, 1800, 2067
Asp718I	GGTACC	1 cut(s)	514
AspA2I	CCTAGG	1 cut(s)	1364
AspLEI	GCGC	3 cut(s)	269, 580, 2883
AsuC2I	CCSGG	2 cut(s)	988, 2864
AsuHPI	GGTGA	4 cut(s)	358, 1484, 1631, 1972
AsuNHI	GCTAGC	1 cut(s)	1399
AvaI	CYCGRG	3 cut(s)	607, 2369, 2454
AvaII	GGWCC	9 cut(s)	572, 603, 1061, 1151, 1172, 1262, 1292, 1322, 2912
AvrII	CCTAGG	1 cut(s)	1364
AxyI	CCTNAGG	1 cut(s)	342
BaeGI	GKGCMC	4 cut(s)	614, 1870, 1964, 2119
BamHI	GGATCC	1 cut(s)	962
BanI	GGYRCC	3 cut(s)	514, 1822, 2746
BanII	GRGCYC	2 cut(s)	614, 854
BauI	CACGAG	1 cut(s)	2238
BbsI	GAAGAC	2 cut(s)	781, 1644
Bbv12I	GWGCWC	6 cut(s)	1870, 1964, 2119, 2322, 2443, 2600
BbvCI	CCTCAGC	1 cut(s)	510
BbvI	GCAGC	5 cut(s)	794, 1244, 1429, 2221, 2255
BceAI	ACGGC	1 cut(s)	839
BcgI	CGANNNNNNTGC	2 cut(s)	2350, 2384
BciVI	GTATCC	1 cut(s)	1023
BclI	TGATCA	3 cut(s)	81, 102, 1531
BcnI	CCSGG	2 cut(s)	988, 2864
BcoDI	GTCTC	3 cut(s)	403, 2561, 2598
BcuI	ACTAGT	1 cut(s)	130
BfaI	CTAG	9 cut(s)	131, 261, 591, 600, 1365, 1400, 1520, 2268, 2895
BfmI	CTRYAG	2 cut(s)	814, 1386
BfuI	GTATCC	1 cut(s)	1023
BglII	AGATCT	2 cut(s)	229, 2842
BisI	GCNGC	6 cut(s)	783, 1233, 1443, 2235, 2244, 2855
BlnI	CCTAGG	1 cut(s)	1364
BlsI	GCNGC	6 cut(s)	784, 1234, 1444, 2236, 2245, 2856
Bme18I	GGWCC	9 cut(s)	572, 603, 1061, 1151, 1172, 1262, 1292, 1322, 2912
BmeT110I	CYCGRG	3 cut(s)	607, 2369, 2454
BmgBI	CACGTC	1 cut(s)	974
BmiI	GGNNCC	9 cut(s)	516, 612, 964, 1152, 1173, 1271, 1301, 1824, 2748
BmrI	ACTGGG	2 cut(s)	202, 1669
BmtI	GCTAGC	1 cut(s)	1403
BmuI	ACTGGG	2 cut(s)	202, 1669
BpiI	GAAGAC	2 cut(s)	781, 1644
BplI	GAGNNNNNCTC	4 cut(s)	401, 433, 2221, 2253
BpmI	CTGGAG	7 cut(s)	423, 482, 903, 1236, 1266, 2360, 2622
Bpu10I	CCTNAGC	3 cut(s)	510, 1110, 1850
BpuEI	CTTGAG	2 cut(s)	2036, 2312
BpuMI	CCSGG	2 cut(s)	988, 2864
BsaBI	GATNNNNATC	1 cut(s)	2847
BsaI	GGTCTC	1 cut(s)	2561
BsaJI	CCNNGG	6 cut(s)	568, 606, 1265, 1295, 1364, 2614
BsaWI	WCCGGW	1 cut(s)	2256
Bse118I	RCCGGY	1 cut(s)	2040
Bse1I	ACTGG	7 cut(s)	151, 208, 303, 1573, 1675, 2083, 2605
Bse21I	CCTNAGG	1 cut(s)	342
Bse3DI	GCAATG	3 cut(s)	741, 1021, 2368
Bse8I	GATNNNNATC	1 cut(s)	2847
BseDI	CCNNGG	6 cut(s)	568, 606, 1265, 1295, 1364, 2614
BseGI	GGATG	9 cut(s)	337, 563, 977, 1007, 1031, 1655, 1917, 2404, 2638
BseJI	GATNNNNATC	1 cut(s)	2847
BseMI	GCAATG	3 cut(s)	741, 1021, 2368
BseNI	ACTGG	7 cut(s)	151, 208, 303, 1573, 1675, 2083, 2605
BseRI	GAGGAG	9 cut(s)	107, 1029, 1059, 1125, 1146, 1167, 1317, 1350, 1988
BseSI	GKGCMC	4 cut(s)	614, 1870, 1964, 2119
BseXI	GCAGC	5 cut(s)	794, 1244, 1429, 2221, 2255
BseYI	CCCAGC	1 cut(s)	689
BsgI	GTGCAG	5 cut(s)	335, 516, 1251, 2262, 2549
Bsh1285I	CGRYCG	1 cut(s)	1639
BshFI	GGCC	6 cut(s)	612, 1051, 1270, 1300, 1369, 1885
BshNI	GGYRCC	3 cut(s)	514, 1822, 2746
BsiEI	CGRYCG	1 cut(s)	1639
BsiHKAI	GWGCWC	6 cut(s)	1870, 1964, 2119, 2322, 2443, 2600
BsiHKCI	CYCGRG	3 cut(s)	607, 2369, 2454
BsiSI	CCGG	4 cut(s)	988, 2041, 2257, 2864
BslFI	GGGAC	2 cut(s)	433, 631
BsmAI	GTCTC	3 cut(s)	403, 2561, 2598
BsmFI	GGGAC	2 cut(s)	433, 631
BsmI	GAATGC	3 cut(s)	418, 2539, 2626
BsnI	GGCC	6 cut(s)	612, 1051, 1270, 1300, 1369, 1885
Bso31I	GGTCTC	1 cut(s)	2561
BsoBI	CYCGRG	3 cut(s)	607, 2369, 2454
Bsp120I	GGGCCC	1 cut(s)	610
Bsp1286I	GDGCHC	8 cut(s)	614, 854, 1870, 1964, 2119, 2322, 2443, 2600
Bsp19I	CCATGG	1 cut(s)	2614
BspACI	CCGC	8 cut(s)	1064, 1148, 1169, 1190, 1304, 2215, 2662, 2854
BspANI	GGCC	6 cut(s)	612, 1051, 1270, 1300, 1369, 1885
BspHI	TCATGA	1 cut(s)	2506
BspLI	GGNNCC	9 cut(s)	516, 612, 964, 1152, 1173, 1271, 1301, 1824, 2748
BspOI	GCTAGC	1 cut(s)	1403
BspQI	GCTCTTC	1 cut(s)	2310
BspT107I	GGYRCC	3 cut(s)	514, 1822, 2746
BspTNI	GGTCTC	1 cut(s)	2561
BsrBI	CCGCTC	1 cut(s)	2217
BsrDI	GCAATG	3 cut(s)	741, 1021, 2368
BsrFI	RCCGGY	1 cut(s)	2040
BsrI	ACTGG	7 cut(s)	151, 208, 303, 1573, 1675, 2083, 2605
BssAI	RCCGGY	1 cut(s)	2040
BssECI	CCNNGG	6 cut(s)	568, 606, 1265, 1295, 1364, 2614
BssSI	CACGAG	1 cut(s)	2238
BssT1I	CCWWGG	2 cut(s)	1364, 2614
Bst2BI	CACGAG	1 cut(s)	2238
Bst4CI	ACNGT	5 cut(s)	294, 766, 1896, 1938, 2422
Bst6I	CTCTTC	4 cut(s)	402, 1971, 2310, 2513
BstC8I	GCNNGC	5 cut(s)	1401, 1887, 2020, 2042, 2828
BstDSI	CCRYGG	1 cut(s)	2614
BstEII	GGTNACC	2 cut(s)	243, 1978
BstENI	CCTNNNNNAGG	1 cut(s)	534
BstF5I	GGATG	9 cut(s)	337, 563, 977, 1007, 1031, 1655, 1917, 2404, 2638
BstHHI	GCGC	3 cut(s)	269, 580, 2883
BstMAI	GTCTC	3 cut(s)	403, 2561, 2598
BstMCI	CGRYCG	1 cut(s)	1639
BstMWI	GCNNNNNNNGC	3 cut(s)	584, 2240, 2243
BstPI	GGTNACC	2 cut(s)	243, 1978
BstSFI	CTRYAG	2 cut(s)	814, 1386
BstSLI	GKGCMC	4 cut(s)	614, 1870, 1964, 2119
BstV1I	GCAGC	5 cut(s)	794, 1244, 1429, 2221, 2255
BstV2I	GAAGAC	2 cut(s)	781, 1644
BstX2I	RGATCY	6 cut(s)	229, 539, 962, 1565, 1663, 2842
BstYI	RGATCY	6 cut(s)	229, 539, 962, 1565, 1663, 2842
Bsu36I	CCTNAGG	1 cut(s)	342
BsuI	GTATCC	1 cut(s)	1023
BsuRI	GGCC	6 cut(s)	612, 1051, 1270, 1300, 1369, 1885
BtgI	CCRYGG	1 cut(s)	2614
BtgZI	GCGATG	1 cut(s)	1776
BtrI	CACGTC	1 cut(s)	974
BtsCI	GGATG	9 cut(s)	337, 563, 977, 1007, 1031, 1655, 1917, 2404, 2638
BtsI	GCAGTG	1 cut(s)	493
BtsIMutI	CAGTG	5 cut(s)	296, 493, 771, 2513, 2598
Cac8I	GCNNGC	5 cut(s)	1401, 1887, 2020, 2042, 2828
CaiI	CAGNNNCTG	6 cut(s)	146, 298, 1259, 1289, 1466, 2420
CciI	TCATGA	1 cut(s)	2506
CfoI	GCGC	3 cut(s)	269, 580, 2883
Cfr10I	RCCGGY	1 cut(s)	2040
CseI	GACGC	2 cut(s)	1418, 2260
Csp6I	GTAC	5 cut(s)	185, 515, 804, 1199, 1355
CviAII	CATG	8 cut(s)	1588, 2089, 2306, 2476, 2507, 2581, 2615, 2884
CviQI	GTAC	5 cut(s)	185, 515, 804, 1199, 1355
DraI	TTTAAA	1 cut(s)	1933
Eam1104I	CTCTTC	4 cut(s)	402, 1971, 2310, 2513
EarI	CTCTTC	4 cut(s)	402, 1971, 2310, 2513
EciI	GGCGGA	1 cut(s)	1179
Eco130I	CCWWGG	2 cut(s)	1364, 2614
Eco147I	AGGCCT	1 cut(s)	1051
Eco24I	GRGCYC	2 cut(s)	614, 854
Eco31I	GGTCTC	1 cut(s)	2561
Eco32I	GATATC	1 cut(s)	2548
Eco47I	GGWCC	9 cut(s)	572, 603, 1061, 1151, 1172, 1262, 1292, 1322, 2912
Eco57I	CTGAAG	5 cut(s)	654, 1094, 1514, 1952, 2537
Eco81I	CCTNAGG	1 cut(s)	342
Eco88I	CYCGRG	3 cut(s)	607, 2369, 2454
Eco91I	GGTNACC	2 cut(s)	243, 1978
EcoNI	CCTNNNNNAGG	1 cut(s)	534
EcoO109I	RGGNCCY	4 cut(s)	603, 611, 1151, 1172
EcoO65I	GGTNACC	2 cut(s)	243, 1978
EcoRI	GAATTC	2 cut(s)	1796, 2482
EcoRV	GATATC	1 cut(s)	2548
EcoT14I	CCWWGG	2 cut(s)	1364, 2614
EcoT38I	GRGCYC	2 cut(s)	614, 854
ErhI	CCWWGG	2 cut(s)	1364, 2614
FaeI	CATG	8 cut(s)	1591, 2092, 2309, 2479, 2510, 2584, 2618, 2887
FalI	AAGNNNNNCTT	4 cut(s)	1935, 1967, 2055, 2087
FaqI	GGGAC	2 cut(s)	433, 631
FatI	CATG	8 cut(s)	1587, 2088, 2305, 2475, 2506, 2580, 2614, 2883
FauI	CCCGC	4 cut(s)	1141, 1162, 1311, 2655
FbaI	TGATCA	3 cut(s)	81, 102, 1531
FblI	GTMKAC	2 cut(s)	127, 2821
Fnu4HI	GCNGC	6 cut(s)	783, 1233, 1443, 2235, 2244, 2855
FokI	GGATG	9 cut(s)	324, 550, 964, 994, 1038, 1642, 1904, 2411, 2645
FriOI	GRGCYC	2 cut(s)	614, 854
Fsp4HI	GCNGC	6 cut(s)	783, 1233, 1443, 2235, 2244, 2855
FspBI	CTAG	9 cut(s)	131, 261, 591, 600, 1365, 1400, 1520, 2268, 2895
GlaI	GCGC	3 cut(s)	268, 579, 2882
GluI	GCNGC	6 cut(s)	783, 1233, 1443, 2235, 2244, 2855
GsaI	CCCAGC	1 cut(s)	693
GsuI	CTGGAG	7 cut(s)	423, 482, 903, 1236, 1266, 2360, 2622
HaeIII	GGCC	6 cut(s)	612, 1051, 1270, 1300, 1369, 1885
HapII	CCGG	4 cut(s)	988, 2041, 2257, 2864
HgaI	GACGC	2 cut(s)	1418, 2260
HhaI	GCGC	3 cut(s)	269, 580, 2883
Hin1II	CATG	8 cut(s)	1591, 2092, 2309, 2479, 2510, 2584, 2618, 2887
Hin6I	GCGC	3 cut(s)	267, 578, 2881
HinP1I	GCGC	3 cut(s)	267, 578, 2881
HindIII	AAGCTT	1 cut(s)	2054
HinfI	GANTC	8 cut(s)	446, 878, 1595, 1649, 1711, 1994, 2577, 2710
HpaII	CCGG	4 cut(s)	988, 2041, 2257, 2864
HphI	GGTGA	4 cut(s)	358, 1484, 1631, 1972
Hpy166II	GTNNAC	8 cut(s)	128, 662, 1355, 1868, 1962, 2117, 2642, 2822
Hpy8I	GTNNAC	8 cut(s)	128, 662, 1355, 1868, 1962, 2117, 2642, 2822
HpyCH4III	ACNGT	5 cut(s)	294, 766, 1896, 1938, 2422
HpyCH4IV	ACGT	2 cut(s)	187, 973
HpyF10VI	GCNNNNNNNGC	3 cut(s)	584, 2240, 2243
HpySE526I	ACGT	2 cut(s)	187, 973
Hsp92II	CATG	8 cut(s)	1591, 2092, 2309, 2479, 2510, 2584, 2618, 2887
HspAI	GCGC	3 cut(s)	267, 578, 2881
KpnI	GGTACC	1 cut(s)	518
KroI	GCCGGC	1 cut(s)	2040
KroNI	GCCGGC	1 cut(s)	2042
Ksp22I	TGATCA	3 cut(s)	81, 102, 1531
LguI	GCTCTTC	1 cut(s)	2310
LmnI	GCTCC	5 cut(s)	501, 691, 1261, 1291, 1483
Lsp1109I	GCAGC	5 cut(s)	794, 1244, 1429, 2221, 2255
MaeI	CTAG	9 cut(s)	131, 261, 591, 600, 1365, 1400, 1520, 2268, 2895
MaeII	ACGT	2 cut(s)	187, 973
MaeIII	GTNAC	8 cut(s)	55, 243, 294, 754, 766, 1741, 1947, 1978
MbiI	CCGCTC	1 cut(s)	2217
MflI	RGATCY	6 cut(s)	229, 539, 962, 1565, 1663, 2842
MhlI	GDGCHC	8 cut(s)	614, 854, 1870, 1964, 2119, 2322, 2443, 2600
MlyI	GAGTC	3 cut(s)	455, 872, 1658
MmeI	TCCRAC	3 cut(s)	900, 1205, 1920
MroNI	GCCGGC	1 cut(s)	2040
MroXI	GAANNNNTTC	4 cut(s)	117, 222, 1800, 2067
MslI	CAYNNNNRTG	1 cut(s)	2619
MspA1I	CMGCKG	1 cut(s)	2854
MspI	CCGG	4 cut(s)	988, 2041, 2257, 2864
Mva1269I	GAATGC	3 cut(s)	418, 2539, 2626
MwoI	GCNNNNNNNGC	3 cut(s)	584, 2240, 2243
NaeI	GCCGGC	1 cut(s)	2042
NciI	CCSGG	2 cut(s)	988, 2864
NcoI	CCATGG	1 cut(s)	2614
NgoMIV	GCCGGC	1 cut(s)	2040
NheI	GCTAGC	1 cut(s)	1399
NlaIII	CATG	8 cut(s)	1591, 2092, 2309, 2479, 2510, 2584, 2618, 2887
NlaIV	GGNNCC	9 cut(s)	516, 612, 964, 1152, 1173, 1271, 1301, 1824, 2748
NmeAIII	GCCGAG	2 cut(s)	1246, 1276
NmuCI	GTSAC	4 cut(s)	294, 766, 1741, 1978
PaeR7I	CTCGAG	1 cut(s)	2369
PagI	TCATGA	1 cut(s)	2506
PceI	AGGCCT	1 cut(s)	1051
PciSI	GCTCTTC	1 cut(s)	2310
PctI	GAATGC	3 cut(s)	418, 2539, 2626
PdiI	GCCGGC	1 cut(s)	2042
PdmI	GAANNNNTTC	4 cut(s)	117, 222, 1800, 2067
PfeI	GAWTC	5 cut(s)	1595, 1711, 1994, 2577, 2710
PflMI	CCANNNNNTGG	7 cut(s)	146, 214, 426, 1226, 1259, 1289, 1349
PfoI	TCCNGGA	1 cut(s)	1042
PkrI	GCNGC	6 cut(s)	784, 1234, 1444, 2236, 2245, 2856
Ple19I	CGATCG	1 cut(s)	1639
PleI	GAGTC	3 cut(s)	454, 872, 1657
PpsI	GAGTC	3 cut(s)	454, 872, 1657
PpuMI	RGGWCCY	3 cut(s)	603, 1151, 1172
PshBI	ATTAAT	1 cut(s)	1692
Psp5II	RGGWCCY	3 cut(s)	603, 1151, 1172
PspEI	GGTNACC	2 cut(s)	243, 1978
PspFI	CCCAGC	1 cut(s)	689
PspN4I	GGNNCC	9 cut(s)	516, 612, 964, 1152, 1173, 1271, 1301, 1824, 2748
PspOMI	GGGCCC	1 cut(s)	610
PspPPI	RGGWCCY	3 cut(s)	603, 1151, 1172
PstNI	CAGNNNCTG	6 cut(s)	146, 298, 1259, 1289, 1466, 2420
PsuI	RGATCY	6 cut(s)	229, 539, 962, 1565, 1663, 2842
PvuI	CGATCG	1 cut(s)	1639
RsaI	GTAC	5 cut(s)	186, 516, 805, 1200, 1356
RsaNI	GTAC	5 cut(s)	185, 515, 804, 1199, 1355
RseI	CAYNNNNRTG	1 cut(s)	2619
SapI	GCTCTTC	1 cut(s)	2310
SatI	GCNGC	6 cut(s)	783, 1233, 1443, 2235, 2244, 2855
SchI	GAGTC	3 cut(s)	455, 872, 1658
SduI	GDGCHC	8 cut(s)	614, 854, 1870, 1964, 2119, 2322, 2443, 2600
SfcI	CTRYAG	2 cut(s)	814, 1386
Sfr274I	CTCGAG	1 cut(s)	2369
SinI	GGWCC	9 cut(s)	572, 603, 1061, 1151, 1172, 1262, 1292, 1322, 2912
SlaI	CTCGAG	1 cut(s)	2369
SmiMI	CAYNNNNRTG	1 cut(s)	2619
SmlI	CTYRAG	3 cut(s)	2051, 2291, 2369
SmoI	CTYRAG	3 cut(s)	2051, 2291, 2369
SpeI	ACTAGT	1 cut(s)	130
SseBI	AGGCCT	1 cut(s)	1051
SsiI	CCGC	8 cut(s)	1064, 1148, 1169, 1190, 1304, 2215, 2662, 2854
SspMI	CTAG	9 cut(s)	131, 261, 591, 600, 1365, 1400, 1520, 2268, 2895
StuI	AGGCCT	1 cut(s)	1051
StyI	CCWWGG	2 cut(s)	1364, 2614
TaaI	ACNGT	5 cut(s)	294, 766, 1896, 1938, 2422
TaiI	ACGT	2 cut(s)	190, 976
TaqI	TCGA	5 cut(s)	656, 1405, 1448, 1914, 2370
TatI	WGTACW	1 cut(s)	803
TauI	GCSGC	1 cut(s)	2857
TfiI	GAWTC	5 cut(s)	1595, 1711, 1994, 2577, 2710
TscAI	CASTG	5 cut(s)	303, 493, 771, 2520, 2605
TseFI	GTSAC	4 cut(s)	294, 766, 1741, 1978
TseI	GCWGC	5 cut(s)	782, 1232, 1442, 2234, 2243
Tsp45I	GTSAC	4 cut(s)	294, 766, 1741, 1978
TspGWI	ACGGA	3 cut(s)	383, 1038, 2549
TspRI	CASTG	5 cut(s)	303, 493, 771, 2520, 2605
Van91I	CCANNNNNTGG	7 cut(s)	146, 214, 426, 1226, 1259, 1289, 1349
VneI	GTGCAC	3 cut(s)	1866, 1960, 2115
VpaK11BI	GGWCC	9 cut(s)	572, 603, 1061, 1151, 1172, 1262, 1292, 1322, 2912
VspI	ATTAAT	1 cut(s)	1692
XagI	CCTNNNNNAGG	1 cut(s)	534
XapI	RAATTY	4 cut(s)	652, 916, 1796, 2482
XhoI	CTCGAG	1 cut(s)	2369
XmaJI	CCTAGG	1 cut(s)	1364
XmiI	GTMKAC	2 cut(s)	127, 2821
XmnI	GAANNNNTTC	4 cut(s)	117, 222, 1800, 2067
XspI	CTAG	9 cut(s)	131, 261, 591, 600, 1365, 1400, 1520, 2268, 2895

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.