MD03G1213300.v1.1

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0009516
Physical Location & Seq
Reverse (-)
29278488 .. 29280559
2072 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1213300.v1.1.491

Sequence Viewer

Length: 627 bp
ATGACTACTAGTAGTACTACGCGGAAGCAGCTTGAAGTCGTAAGAGACGATCAAACTCCGCAGAAATGGTGTGTTTTATTGAGAGAAGACGTGTTCAGAAGATTCATGTCTCAGGGCAGTCCAAATCCAACAATCCAGAAGGTCTTTGGTGGTGCATCTTTTTTCAGTCCTTTGTTGTTTGGGAAATTTTTTGATCCTTCTGATGCCTTCCCACTGTGGGAGTTTGAGTCAGATATCTTGCTGTCTGGTCTCAGAAGCGCTGGCCAAAACACTATTGATTGGTTTCAAACAGATCGAGACTATGTCCTTAAAGTAGAGCTACCAGGAGAAGGGAAAAATAATATTCAGGTGTATGCTGAAAATGGGAAGGTGGTGGAAATTAGCGGGCAGTGGAAGCAGCAGCAGCAAGGAGGAGAGTCCAACTCCTTGAAGAGCACAAAGGATTGGAGAAGTGGGAATTGGTGGGAACATGGTTATGTTCGGAGGCTTGAGCTCCCACAAGATGCAGATTCCAAAAGGATAGAGGCTTCTCTCATTAATGACCTACTTTTGGAAATCAAAATTCACAAGATCAACAACAAACCCTTGGATTATGAGGCTAATGCAAAAGATAATGATGCGGTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

209

Amino Acids

23.86

Weight (kDa)

5.34

Isoelectric Point (pI)

48.67

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 93 - 194 1.7e-09 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 22
AciI CCGC 4 cut(s) 22, 59, 384, 620
AclWI GGATC 1 cut(s) 188
AcoI YGGCCR 1 cut(s) 262
AcsI RAATTY 2 cut(s) 185, 561
AfaI GTAC 1 cut(s) 16
AfeI AGCGCT 1 cut(s) 259
AfiI CCNNNNNNNGG 3 cut(s) 217, 329, 550
AflIII ACRYGT 1 cut(s) 90
AgsI TTSAA 3 cut(s) 35, 287, 430
AhlI ACTAGT 1 cut(s) 8
AjiI CACGTC 1 cut(s) 91
AjnI CCWGG 1 cut(s) 322
AjuI GAANNNNNNNTTGG 2 cut(s) 442, 474
AluBI AGCT 3 cut(s) 31, 319, 493
AluI AGCT 3 cut(s) 31, 319, 493
Alw21I GWGCWC 2 cut(s) 437, 495
Alw26I GTCTC 4 cut(s) 39, 114, 254, 291
AlwI GGATC 1 cut(s) 188
Aor51HI AGCGCT 1 cut(s) 259
AoxI GGCC 1 cut(s) 262
ApeKI GCWGC 4 cut(s) 28, 397, 400, 403
ApoI RAATTY 2 cut(s) 185, 561
AseI ATTAAT 1 cut(s) 537
AspLEI GCGC 1 cut(s) 260
BalI TGGCCA 1 cut(s) 264
BanII GRGCYC 1 cut(s) 495
BbsI GAAGAC 1 cut(s) 93
Bbv12I GWGCWC 2 cut(s) 437, 495
BbvI GCAGC 4 cut(s) 40, 409, 412, 415
BciT130I CCWGG 1 cut(s) 324
BcoDI GTCTC 4 cut(s) 39, 114, 254, 291
BcuI ACTAGT 1 cut(s) 8
BfaI CTAG 1 cut(s) 9
BfoI RGCGCY 1 cut(s) 261
BisI GCNGC 4 cut(s) 29, 398, 401, 404
BlsI GCNGC 4 cut(s) 30, 399, 402, 405
BmcAI AGTACT 1 cut(s) 16
Bme1390I CCNGG 1 cut(s) 324
BmgBI CACGTC 1 cut(s) 91
BmrFI CCNGG 1 cut(s) 324
BmsI GCATC 4 cut(s) 164, 193, 493, 607
BpiI GAAGAC 1 cut(s) 93
BplI GAGNNNNNCTC 2 cut(s) 407, 439
BpuEI CTTGAG 1 cut(s) 509
BsaI GGTCTC 1 cut(s) 254
BsaJI CCNNGG 1 cut(s) 585
BsaXI ACNNNNNCTCC 2 cut(s) 212, 242
Bsc4I CCNNNNNNNGG 3 cut(s) 217, 329, 550
BseBI CCWGG 1 cut(s) 324
BseDI CCNNGG 1 cut(s) 585
BseLI CCNNNNNNNGG 3 cut(s) 217, 329, 550
BseMII CTCAG 2 cut(s) 125, 265
BseRI GAGGAG 1 cut(s) 426
BseXI GCAGC 4 cut(s) 40, 409, 412, 415
Bsh1236I CGCG 1 cut(s) 22
BshFI GGCC 1 cut(s) 264
BsiHKAI GWGCWC 2 cut(s) 437, 495
BslI CCNNNNNNNGG 3 cut(s) 217, 329, 550
BsmAI GTCTC 4 cut(s) 39, 114, 254, 291
BsmBI CGTCTC 1 cut(s) 39
BsnI GGCC 1 cut(s) 264
Bso31I GGTCTC 1 cut(s) 254
Bsp1286I GDGCHC 2 cut(s) 437, 495
Bsp143I GATC 4 cut(s) 49, 193, 292, 570
BspACI CCGC 4 cut(s) 22, 59, 384, 620
BspANI GGCC 1 cut(s) 264
BspCNI CTCAG 2 cut(s) 124, 264
BspFNI CGCG 1 cut(s) 22
BspPI GGATC 1 cut(s) 188
BspQI GCTCTTC 1 cut(s) 425
BspTNI GGTCTC 1 cut(s) 254
BssECI CCNNGG 1 cut(s) 585
BssMI GATC 4 cut(s) 49, 193, 292, 570
BssT1I CCWWGG 1 cut(s) 585
Bst2UI CCWGG 1 cut(s) 324
Bst4CI ACNGT 1 cut(s) 216
Bst6I CTCTTC 1 cut(s) 425
BstC8I GCNNGC 2 cut(s) 262, 386
BstDEI CTNAG 2 cut(s) 111, 251
BstFNI CGCG 1 cut(s) 22
BstH2I RGCGCY 1 cut(s) 261
BstHHI GCGC 1 cut(s) 260
BstKTI GATC 4 cut(s) 52, 196, 295, 573
BstMAI GTCTC 4 cut(s) 39, 114, 254, 291
BstMBI GATC 4 cut(s) 49, 193, 292, 570
BstMWI GCNNNNNNNGC 3 cut(s) 28, 394, 403
BstNI CCWGG 1 cut(s) 324
BstSCI CCNGG 1 cut(s) 322
BstUI CGCG 1 cut(s) 22
BstV1I GCAGC 4 cut(s) 40, 409, 412, 415
BstV2I GAAGAC 1 cut(s) 93
BsuRI GGCC 1 cut(s) 264
BtrI CACGTC 1 cut(s) 91
BtsI GCAGTG 1 cut(s) 395
BtsIMutI CAGTG 2 cut(s) 212, 395
Cac8I GCNNGC 2 cut(s) 262, 386
CfoI GCGC 1 cut(s) 260
Csp6I GTAC 1 cut(s) 15
CviAII CATG 2 cut(s) 106, 470
CviJI RGCY 7 cut(s) 31, 264, 319, 487, 493, 527, 599
CviKI_1 RGCY 7 cut(s) 31, 264, 319, 487, 493, 527, 599
CviQI GTAC 1 cut(s) 15
DdeI CTNAG 2 cut(s) 111, 251
DpnI GATC 4 cut(s) 51, 195, 294, 572
DpnII GATC 4 cut(s) 49, 193, 292, 570
EaeI YGGCCR 1 cut(s) 262
Eam1104I CTCTTC 1 cut(s) 425
EarI CTCTTC 1 cut(s) 425
Ecl136II GAGCTC 1 cut(s) 493
Eco130I CCWWGG 1 cut(s) 585
Eco24I GRGCYC 1 cut(s) 495
Eco31I GGTCTC 1 cut(s) 254
Eco32I GATATC 1 cut(s) 235
Eco47III AGCGCT 1 cut(s) 259
Eco53kI GAGCTC 1 cut(s) 493
EcoICRI GAGCTC 1 cut(s) 493
EcoRII CCWGG 1 cut(s) 322
EcoRV GATATC 1 cut(s) 235
EcoT14I CCWWGG 1 cut(s) 585
EcoT38I GRGCYC 1 cut(s) 495
ErhI CCWWGG 1 cut(s) 585
Esp3I CGTCTC 1 cut(s) 39
FaeI CATG 2 cut(s) 109, 473
FaiI YATR 6 cut(s) 107, 303, 354, 471, 477, 594
FatI CATG 2 cut(s) 105, 469
FauI CCCGC 1 cut(s) 377
Fnu4HI GCNGC 4 cut(s) 29, 398, 401, 404
FriOI GRGCYC 1 cut(s) 495
Fsp4HI GCNGC 4 cut(s) 29, 398, 401, 404
FspBI CTAG 1 cut(s) 9
GlaI GCGC 1 cut(s) 259
GluI GCNGC 4 cut(s) 29, 398, 401, 404
HaeII RGCGCY 1 cut(s) 261
HaeIII GGCC 1 cut(s) 264
HhaI GCGC 1 cut(s) 260
Hin1II CATG 2 cut(s) 109, 473
Hin6I GCGC 1 cut(s) 258
HinP1I GCGC 1 cut(s) 258
HinfI GANTC 4 cut(s) 102, 227, 416, 509
Hpy188I TCNGA 5 cut(s) 98, 202, 232, 254, 483
Hpy188III TCNNGA 2 cut(s) 136, 296
HpyAV CCTTC 5 cut(s) 133, 207, 217, 323, 361
HpyCH4III ACNGT 1 cut(s) 216
HpyCH4IV ACGT 1 cut(s) 90
HpyCH4V TGCA 3 cut(s) 155, 506, 605
HpyF10VI GCNNNNNNNGC 3 cut(s) 28, 394, 403
HpyF3I CTNAG 2 cut(s) 111, 251
HpySE526I ACGT 1 cut(s) 90
Hsp92II CATG 2 cut(s) 109, 473
HspAI GCGC 1 cut(s) 258
Kzo9I GATC 4 cut(s) 49, 193, 292, 570
LguI GCTCTTC 1 cut(s) 425
LmnI GCTCC 1 cut(s) 498
LpnPI CCDG 7 cut(s) 98, 149, 231, 246, 309, 332, 336
Lsp1109I GCAGC 4 cut(s) 40, 409, 412, 415
LweI GCATC 4 cut(s) 164, 193, 493, 607
MaeI CTAG 1 cut(s) 9
MaeII ACGT 1 cut(s) 90
MalI GATC 4 cut(s) 51, 195, 294, 572
MboI GATC 4 cut(s) 49, 193, 292, 570
MboII GAAGA 3 cut(s) 98, 111, 442
MhlI GDGCHC 2 cut(s) 437, 495
MlsI TGGCCA 1 cut(s) 264
MluCI AATT 4 cut(s) 185, 378, 457, 561
MluNI TGGCCA 1 cut(s) 264
MlyI GAGTC 2 cut(s) 236, 425
MmeI TCCRAC 2 cut(s) 152, 444
MnlI CCTC 4 cut(s) 404, 477, 517, 589
Mox20I TGGCCA 1 cut(s) 264
MscI TGGCCA 1 cut(s) 264
MseI TTAA 2 cut(s) 309, 537
MslI CAYNNNNRTG 1 cut(s) 474
Msp20I TGGCCA 1 cut(s) 264
MspR9I CCNGG 1 cut(s) 324
MvaI CCWGG 1 cut(s) 324
MvnI CGCG 1 cut(s) 22
MwoI GCNNNNNNNGC 3 cut(s) 28, 394, 403
NdeII GATC 4 cut(s) 49, 193, 292, 570
NlaIII CATG 2 cut(s) 109, 473
PciSI GCTCTTC 1 cut(s) 425
PcsI WCGNNNNNNNCGW 1 cut(s) 45
PfeI GAWTC 2 cut(s) 102, 509
PflFI GACNNNGTC 1 cut(s) 302
PkrI GCNGC 4 cut(s) 30, 399, 402, 405
PleI GAGTC 2 cut(s) 235, 424
PpsI GAGTC 2 cut(s) 235, 424
PshBI ATTAAT 1 cut(s) 537
Psp124BI GAGCTC 1 cut(s) 495
Psp6I CCWGG 1 cut(s) 322
PspGI CCWGG 1 cut(s) 322
PsyI GACNNNGTC 1 cut(s) 302
RsaI GTAC 1 cut(s) 16
RsaNI GTAC 1 cut(s) 15
RseI CAYNNNNRTG 1 cut(s) 474
SacI GAGCTC 1 cut(s) 495
SapI GCTCTTC 1 cut(s) 425
SaqAI TTAA 2 cut(s) 309, 537
SatI GCNGC 4 cut(s) 29, 398, 401, 404
Sau3AI GATC 4 cut(s) 49, 193, 292, 570
ScaI AGTACT 1 cut(s) 16
SchI GAGTC 2 cut(s) 236, 425
ScrFI CCNGG 1 cut(s) 324
SduI GDGCHC 2 cut(s) 437, 495
SetI ASST 8 cut(s) 33, 93, 144, 321, 351, 372, 495, 546
SfaNI GCATC 4 cut(s) 164, 193, 493, 607
SmiMI CAYNNNNRTG 1 cut(s) 474
SmlI CTYRAG 1 cut(s) 488
SmoI CTYRAG 1 cut(s) 488
SpeI ACTAGT 1 cut(s) 8
Sse9I AATT 4 cut(s) 185, 378, 457, 561
SsiI CCGC 4 cut(s) 22, 59, 384, 620
SspI AATATT 1 cut(s) 343
SspMI CTAG 1 cut(s) 9
SstI GAGCTC 1 cut(s) 495
StyD4I CCNGG 1 cut(s) 322
StyI CCWWGG 1 cut(s) 585
TaaI ACNGT 1 cut(s) 216
TaiI ACGT 1 cut(s) 93
TaqI TCGA 1 cut(s) 295
TasI AATT 4 cut(s) 185, 378, 457, 561
TatI WGTACW 1 cut(s) 14
TfiI GAWTC 2 cut(s) 102, 509
Tru1I TTAA 2 cut(s) 309, 537
Tru9I TTAA 2 cut(s) 309, 537
TscAI CASTG 2 cut(s) 219, 395
TseI GCWGC 4 cut(s) 28, 397, 400, 403
TspDTI ATGAA 1 cut(s) 94
TspRI CASTG 2 cut(s) 219, 395
Tth111I GACNNNGTC 1 cut(s) 302
VspI ATTAAT 1 cut(s) 537
XapI RAATTY 2 cut(s) 185, 561
XcmI CCANNNNNNNNNTGG 1 cut(s) 143
XspI CTAG 1 cut(s) 9
ZrmI AGTACT 1 cut(s) 16
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.