FvH4_3g21680

Belongs to the small heat shock protein (HSP20) family

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0009516
Physical Location & Seq
Forward (+)
14755636 .. 14759019
3384 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g21680.t1

Sequence Viewer

Length: 591 bp
ATGACTACCTCTACTCACAAGCAGCTTGAAGTTTTTACAGATGATCACACTCCACAGAAATGGTGTGTTCTATTGAGAGAAGAAGTGTTCAAGAAGTTCATGTCTCAAGGTAGTCCAATAGTGCACAAGGTGTTTGGTAGTGGATCATTTTTCAGTCCTCTTTTGTTTGGGAAATTCTTTGATCCTTCTGATGCTTTTCCACTGTGGGAGTTTGAGTCGGATATCTTATTGTCTAGTCTAAGAAGCTCTGGCCAGAGCACTATTGATTGGTCTCAGACAGATCAAGATTATGTGCTCAAAGCAGAACTACCAGGAGAAGGGAAAAACAGTGTTCAGGTGTATGCTGAAAATGGGAAGGTTTTGGAGATTAGTGGGCAGTGGAAGCAGCAGCAGCCCAAGACAAACAGTGCAAGAGATTGGAGATGTGGGCATTGGTGGGAATCTGGGTATGTTAGGAGGCTTGAGCTCCCACAAGATGCAGATTGGAGAAAGATAGAGGCATATGTTACAAATGACCAGATTCTGGAAATCAAAATTCACAAGATCAACAACCCCTTGGATTCTGATAATAATCAGAACACTACTGCATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko04141 map04141
Pfam Domains
Protein Families
HSP20

Protein Analysis

197

Amino Acids

22.62

Weight (kDa)

5.38

Isoelectric Point (pI)

48.97

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
HSP20 PF00011 89 - 185 2.4e-09 Hsp20/alpha crystallin family
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 523
AclWI GGATC 2 cut(s) 151, 176
AcoI YGGCCR 1 cut(s) 250
AcsI RAATTY 2 cut(s) 173, 534
AdeI CACNNNGTG 1 cut(s) 130
AfiI CCNNNNNNNGG 2 cut(s) 317, 523
AgsI TTSAA 2 cut(s) 29, 91
AjnI CCWGG 1 cut(s) 310
AluBI AGCT 3 cut(s) 25, 246, 466
AluI AGCT 3 cut(s) 25, 246, 466
Alw21I GWGCWC 4 cut(s) 126, 260, 297, 468
Alw26I GTCTC 2 cut(s) 108, 276
Alw44I GTGCAC 1 cut(s) 122
AlwI GGATC 2 cut(s) 151, 176
AlwNI CAGNNNCTG 1 cut(s) 523
AoxI GGCC 1 cut(s) 250
ApaLI GTGCAC 1 cut(s) 122
ApeKI GCWGC 4 cut(s) 22, 385, 388, 391
ApoI RAATTY 2 cut(s) 173, 534
BaeGI GKGCMC 1 cut(s) 126
BalI TGGCCA 1 cut(s) 252
BanII GRGCYC 1 cut(s) 468
Bbv12I GWGCWC 4 cut(s) 126, 260, 297, 468
BbvI GCAGC 4 cut(s) 34, 397, 400, 403
BciT130I CCWGG 1 cut(s) 312
BclI TGATCA 1 cut(s) 43
BcoDI GTCTC 2 cut(s) 108, 276
BfaI CTAG 1 cut(s) 234
BisI GCNGC 4 cut(s) 23, 386, 389, 392
BlsI GCNGC 4 cut(s) 24, 387, 390, 393
Bme1390I CCNGG 1 cut(s) 312
BmrFI CCNGG 1 cut(s) 312
BmsI GCATC 2 cut(s) 181, 466
BpuEI CTTGAG 2 cut(s) 90, 482
BsaBI GATNNNNATC 1 cut(s) 570
BsaI GGTCTC 1 cut(s) 276
BsaJI CCNNGG 1 cut(s) 555
BsaXI ACNNNNNCTCC 2 cut(s) 200, 230
Bsc4I CCNNNNNNNGG 2 cut(s) 317, 523
Bse8I GATNNNNATC 1 cut(s) 570
BseBI CCWGG 1 cut(s) 312
BseDI CCNNGG 1 cut(s) 555
BseJI GATNNNNATC 1 cut(s) 570
BseLI CCNNNNNNNGG 2 cut(s) 317, 523
BseMII CTCAG 1 cut(s) 287
BseSI GKGCMC 1 cut(s) 126
BseXI GCAGC 4 cut(s) 34, 397, 400, 403
BshFI GGCC 1 cut(s) 252
BsiHKAI GWGCWC 4 cut(s) 126, 260, 297, 468
BslI CCNNNNNNNGG 2 cut(s) 317, 523
BsmAI GTCTC 2 cut(s) 108, 276
BsnI GGCC 1 cut(s) 252
Bso31I GGTCTC 1 cut(s) 276
Bsp1286I GDGCHC 4 cut(s) 126, 260, 297, 468
Bsp143I GATC 5 cut(s) 43, 143, 181, 280, 543
BspANI GGCC 1 cut(s) 252
BspCNI CTCAG 1 cut(s) 286
BspPI GGATC 2 cut(s) 151, 176
BspTNI GGTCTC 1 cut(s) 276
BssECI CCNNGG 1 cut(s) 555
BssMI GATC 5 cut(s) 43, 143, 181, 280, 543
BssT1I CCWWGG 1 cut(s) 555
Bst2UI CCWGG 1 cut(s) 312
Bst4CI ACNGT 3 cut(s) 204, 329, 407
BstDEI CTNAG 2 cut(s) 239, 273
BstKTI GATC 5 cut(s) 46, 146, 184, 283, 546
BstMAI GTCTC 2 cut(s) 108, 276
BstMBI GATC 5 cut(s) 43, 143, 181, 280, 543
BstMWI GCNNNNNNNGC 2 cut(s) 382, 391
BstNI CCWGG 1 cut(s) 312
BstSCI CCNGG 1 cut(s) 310
BstSLI GKGCMC 1 cut(s) 126
BstV1I GCAGC 4 cut(s) 34, 397, 400, 403
BstXI CCANNNNNNTGG 1 cut(s) 60
BsuRI GGCC 1 cut(s) 252
BtsI GCAGTG 1 cut(s) 383
BtsIMutI CAGTG 4 cut(s) 200, 334, 383, 412
CaiI CAGNNNCTG 1 cut(s) 523
CviAII CATG 1 cut(s) 100
CviJI RGCY 6 cut(s) 25, 246, 252, 394, 460, 466
CviKI_1 RGCY 6 cut(s) 25, 246, 252, 394, 460, 466
DdeI CTNAG 2 cut(s) 239, 273
DpnI GATC 5 cut(s) 45, 145, 183, 282, 545
DpnII GATC 5 cut(s) 43, 143, 181, 280, 543
DraIII CACNNNGTG 1 cut(s) 130
EaeI YGGCCR 1 cut(s) 250
Ecl136II GAGCTC 1 cut(s) 466
Eco130I CCWWGG 1 cut(s) 555
Eco24I GRGCYC 1 cut(s) 468
Eco31I GGTCTC 1 cut(s) 276
Eco32I GATATC 1 cut(s) 223
Eco53kI GAGCTC 1 cut(s) 466
EcoICRI GAGCTC 1 cut(s) 466
EcoRII CCWGG 1 cut(s) 310
EcoRV GATATC 1 cut(s) 223
EcoT14I CCWWGG 1 cut(s) 555
EcoT38I GRGCYC 1 cut(s) 468
ErhI CCWWGG 1 cut(s) 555
FaeI CATG 1 cut(s) 103
FaiI YATR 7 cut(s) 101, 291, 342, 450, 502, 504, 589
FatI CATG 1 cut(s) 99
FauNDI CATATG 1 cut(s) 502
FbaI TGATCA 1 cut(s) 43
Fnu4HI GCNGC 4 cut(s) 23, 386, 389, 392
FriOI GRGCYC 1 cut(s) 468
Fsp4HI GCNGC 4 cut(s) 23, 386, 389, 392
FspBI CTAG 1 cut(s) 234
GluI GCNGC 4 cut(s) 23, 386, 389, 392
HaeIII GGCC 1 cut(s) 252
Hin1II CATG 1 cut(s) 103
HinfI GANTC 4 cut(s) 215, 440, 520, 560
Hpy166II GTNNAC 1 cut(s) 124
Hpy188I TCNGA 5 cut(s) 190, 220, 276, 565, 576
Hpy188III TCNNGA 3 cut(s) 91, 284, 524
Hpy8I GTNNAC 1 cut(s) 124
HpyAV CCTTC 3 cut(s) 195, 311, 349
HpyCH4III ACNGT 3 cut(s) 204, 329, 407
HpyCH4V TGCA 4 cut(s) 124, 410, 479, 587
HpyF10VI GCNNNNNNNGC 2 cut(s) 382, 391
HpyF3I CTNAG 2 cut(s) 239, 273
Hsp92II CATG 1 cut(s) 103
Ksp22I TGATCA 1 cut(s) 43
Kzo9I GATC 5 cut(s) 43, 143, 181, 280, 543
LmnI GCTCC 1 cut(s) 471
LpnPI CCDG 8 cut(s) 234, 266, 297, 320, 324, 429, 509, 530
Lsp1109I GCAGC 4 cut(s) 34, 397, 400, 403
LweI GCATC 2 cut(s) 181, 466
MaeI CTAG 1 cut(s) 234
MaeIII GTNAC 1 cut(s) 505
MalI GATC 5 cut(s) 45, 145, 183, 282, 545
MboI GATC 5 cut(s) 43, 143, 181, 280, 543
MboII GAAGA 1 cut(s) 92
MhlI GDGCHC 4 cut(s) 126, 260, 297, 468
MlsI TGGCCA 1 cut(s) 252
MluCI AATT 2 cut(s) 173, 534
MluNI TGGCCA 1 cut(s) 252
MlyI GAGTC 1 cut(s) 224
MmeI TCCRAC 1 cut(s) 198
MnlI CCTC 4 cut(s) 19, 168, 450, 490
Mox20I TGGCCA 1 cut(s) 252
MscI TGGCCA 1 cut(s) 252
MslI CAYNNNNRTG 1 cut(s) 58
Msp20I TGGCCA 1 cut(s) 252
MspR9I CCNGG 1 cut(s) 312
MvaI CCWGG 1 cut(s) 312
MwoI GCNNNNNNNGC 2 cut(s) 382, 391
NdeI CATATG 1 cut(s) 502
NdeII GATC 5 cut(s) 43, 143, 181, 280, 543
NlaIII CATG 1 cut(s) 103
PfeI GAWTC 3 cut(s) 440, 520, 560
PflMI CCANNNNNTGG 1 cut(s) 523
PkrI GCNGC 4 cut(s) 24, 387, 390, 393
PleI GAGTC 1 cut(s) 223
PpsI GAGTC 1 cut(s) 223
Psp124BI GAGCTC 1 cut(s) 468
Psp6I CCWGG 1 cut(s) 310
PspGI CCWGG 1 cut(s) 310
PstNI CAGNNNCTG 1 cut(s) 523
RseI CAYNNNNRTG 1 cut(s) 58
SacI GAGCTC 1 cut(s) 468
SatI GCNGC 4 cut(s) 23, 386, 389, 392
Sau3AI GATC 5 cut(s) 43, 143, 181, 280, 543
SchI GAGTC 1 cut(s) 224
ScrFI CCNGG 1 cut(s) 312
SduI GDGCHC 4 cut(s) 126, 260, 297, 468
SetI ASST 8 cut(s) 11, 27, 112, 132, 248, 339, 360, 468
SfaNI GCATC 2 cut(s) 181, 466
SmiMI CAYNNNNRTG 1 cut(s) 58
SmlI CTYRAG 2 cut(s) 105, 461
SmoI CTYRAG 2 cut(s) 105, 461
Sse9I AATT 2 cut(s) 173, 534
SspMI CTAG 1 cut(s) 234
SstI GAGCTC 1 cut(s) 468
StyD4I CCNGG 1 cut(s) 310
StyI CCWWGG 1 cut(s) 555
TaaI ACNGT 3 cut(s) 204, 329, 407
TasI AATT 2 cut(s) 173, 534
TfiI GAWTC 3 cut(s) 440, 520, 560
TscAI CASTG 4 cut(s) 207, 334, 383, 412
TseI GCWGC 4 cut(s) 22, 385, 388, 391
TspDTI ATGAA 1 cut(s) 88
TspRI CASTG 4 cut(s) 207, 334, 383, 412
Van91I CCANNNNNTGG 1 cut(s) 523
VneI GTGCAC 1 cut(s) 122
XapI RAATTY 2 cut(s) 173, 534
XspI CTAG 1 cut(s) 234
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.