AT5G55250

SAM dependent carboxyl methyltransferase

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008751
Physical Location & Seq
Reverse (-)
22407427 .. 22410974
3548 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G55250.2

Sequence Viewer

Length: 1161 bp
ATGGGTTCTAAGGGAGACAACGTTGCTGTATGTAACATGAAGCTCGAGAGACTTCTCAGCATGAAAGGTGGCAAAGGACAAGACAGCTACGCCAATAACTCTCAAGCTCAGGCAATGCATGCACGGTCCATGCTTCACCTCCTAGAGGAAACACTTGAAAATGTTCACCTCAACTCCTCCGCCAGTCCGCCACCGTTCACGGCGGTTGATCTTGGCTGTTCCTCCGGCGCAAATACTGTCCACATAATCGATTTCATAGTCAAACATATCTCTAAGAGGTTTGATGCTGCCGGAATCGACCCTCCGGAGTTCACAGCTTTCTTCTCCGATCTCCCAAGCAACGACTTCAACACGCTTTTCCAGCTTCTTCCACCACTTGTCTCTAACACTTGCATGGAGGAGTGTCTTGCTGCCGACGGGAACCGCTCCTACTTCGTTGCTGGTGTTCCCGGATCGTTTTACCGGCGACTTTTTCCAGCGAGAACCATTGACTTTTTCCACTCTGCCTTCTCCTTGCATTGGCTCTCTCAGGTGCCGGAAAGTGTGACGGATAGGAGATCGGCGGCGTACAATAGAGGGAGAGTTTTCATACACGGTGCCGGAGAGAAGACAACAACGGCATACAAACGGCAGTTTCAGGCCGACTTAGCGGAGTTTTTGAGGGCAAGAGCGGCGGAGGTTAAGAGAGGTGGGGCCATGTTCCTGGTCTGTCTTGGTCGTACCTCTGTGGATCCCACCGACCAGGGTGGTGCCGGTCTACTCTTCGGCACTCATTTTCAGGACGCTTGGGACGACCTTGTCCGCGAGGGTTTGGTAGCAGCAGAGAAACGAGATGGATTCAATATCCCGGTGTACGCACCAAGCTTGCAAGATTTCAAGGAAGTAGTTGACGCCAACGGCTCATTTGCAATTGATAAGCTGGTAGTATACAAAGGAGGGAGTCCACTTGTGGTGAATGAGCCAGACGATGCATCAGAAGTTGGTCGTGCCTTTGCTAGTAGTTGTCGGAGTGTGGCCGGAGTGCTCGTGGAAGCTCACATAGGCGAAGAGCTAAGCAATAAACTGTTCTCACGAGTTGAAAGCCGAGCCACTAGCCATGCCAAAGATGTCTTGGTGAATTTGCAGTTCTTCCACATTGTCGCTTCTTTGTCTTTTACTTAA

Protein Analysis

386

Amino Acids

42.05

Weight (kDa)

5.95

Isoelectric Point (pI)

48.25

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 14 - 345 5.5e-101 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 797
AccB1I GGYRCC 3 cut(s) 532, 596, 749
AccBSI CCGCTC 2 cut(s) 426, 671
AccI GTMKAC 2 cut(s) 757, 927
AccII CGCG 1 cut(s) 804
AccIII TCCGGA 1 cut(s) 304
AciI CCGC 9 cut(s) 180, 188, 203, 424, 563, 650, 671, 674, 802
AclI AACGTT 1 cut(s) 21
AclWI GGATC 3 cut(s) 460, 725, 738
AcoI YGGCCR 1 cut(s) 1014
AcsI RAATTY 1 cut(s) 1117
AcyI GRCGYC 1 cut(s) 891
AfaI GTAC 3 cut(s) 569, 721, 854
AfiI CCNNNNNNNGG 2 cut(s) 145, 519
AgsI TTSAA 5 cut(s) 158, 349, 841, 877, 1079
AjnI CCWGG 2 cut(s) 702, 741
AluBI AGCT 9 cut(s) 43, 87, 107, 317, 364, 864, 919, 1034, 1051
AluI AGCT 9 cut(s) 43, 87, 107, 317, 364, 864, 919, 1034, 1051
Alw21I GWGCWC 1 cut(s) 1026
Alw26I GTCTC 3 cut(s) 9, 43, 385
AlwI GGATC 3 cut(s) 460, 725, 738
Ama87I CYCGRG 1 cut(s) 44
Aor13HI TCCGGA 1 cut(s) 304
AoxI GGCC 3 cut(s) 639, 693, 1014
ApeKI GCWGC 3 cut(s) 287, 410, 818
ApoI RAATTY 1 cut(s) 1117
Asp700I GAANNNNTTC 1 cut(s) 162
AspLEI GCGC 1 cut(s) 230
AspS9I GGNCC 2 cut(s) 126, 693
AsuC2I CCSGG 2 cut(s) 450, 848
AsuHPI GGTGA 4 cut(s) 128, 158, 964, 1126
AvaI CYCGRG 1 cut(s) 44
AvaII GGWCC 1 cut(s) 126
BamHI GGATCC 1 cut(s) 730
BanI GGYRCC 3 cut(s) 532, 596, 749
BauI CACGAG 2 cut(s) 1025, 1071
BbsI GAAGAC 1 cut(s) 614
Bbv12I GWGCWC 1 cut(s) 1026
BbvI GCAGC 3 cut(s) 274, 397, 830
BccI CCATC 1 cut(s) 827
BceAI ACGGC 4 cut(s) 216, 633, 644, 913
BciT130I CCWGG 2 cut(s) 704, 743
BcnI CCSGG 2 cut(s) 450, 848
BcoDI GTCTC 3 cut(s) 9, 43, 385
BfaI CTAG 3 cut(s) 143, 996, 1092
BisI GCNGC 5 cut(s) 288, 411, 564, 672, 819
BlpI GCTNAGC 1 cut(s) 1052
BlsI GCNGC 5 cut(s) 289, 412, 565, 673, 820
Bme1390I CCNGG 4 cut(s) 450, 704, 743, 848
Bme18I GGWCC 1 cut(s) 126
BmeT110I CYCGRG 1 cut(s) 44
BmgT120I GGNCC 2 cut(s) 126, 693
BmiI GGNNCC 6 cut(s) 422, 534, 598, 694, 732, 751
BmrFI CCNGG 4 cut(s) 450, 704, 743, 848
BmsI GCATC 3 cut(s) 274, 958, 980
BpiI GAAGAC 1 cut(s) 614
Bpu10I CCTNAGC 1 cut(s) 108
Bpu1102I GCTNAGC 1 cut(s) 1052
BpuEI CTTGAG 1 cut(s) 87
BpuMI CCSGG 2 cut(s) 450, 848
Bsa29I ATCGAT 1 cut(s) 249
BsaHI GRCGYC 1 cut(s) 891
BsaJI CCNNGG 1 cut(s) 742
BsaWI WCCGGW 1 cut(s) 304
BsaXI ACNNNNNCTCC 6 cut(s) 6, 36, 158, 188, 1011, 1041
Bsc4I CCNNNNNNNGG 2 cut(s) 145, 519
Bse118I RCCGGY 2 cut(s) 462, 752
Bse1I ACTGG 1 cut(s) 183
Bse3DI GCAATG 1 cut(s) 120
BseAI TCCGGA 1 cut(s) 304
BseBI CCWGG 2 cut(s) 704, 743
BseCI ATCGAT 1 cut(s) 249
BseDI CCNNGG 1 cut(s) 742
BseLI CCNNNNNNNGG 2 cut(s) 145, 519
BseMI GCAATG 1 cut(s) 120
BseMII CTCAG 3 cut(s) 70, 122, 542
BseNI ACTGG 1 cut(s) 183
BseRI GAGGAG 2 cut(s) 166, 413
BseXI GCAGC 3 cut(s) 274, 397, 830
Bsh1236I CGCG 1 cut(s) 804
BshFI GGCC 3 cut(s) 641, 695, 1016
BshNI GGYRCC 3 cut(s) 532, 596, 749
BshVI ATCGAT 1 cut(s) 249
BsiHKAI GWGCWC 1 cut(s) 1026
BsiHKCI CYCGRG 1 cut(s) 44
BslFI GGGAC 1 cut(s) 803
BslI CCNNNNNNNGG 2 cut(s) 145, 519
BsmAI GTCTC 3 cut(s) 9, 43, 385
BsmFI GGGAC 1 cut(s) 803
BsnI GGCC 3 cut(s) 641, 695, 1016
BsoBI CYCGRG 1 cut(s) 44
Bsp1286I GDGCHC 1 cut(s) 1026
Bsp13I TCCGGA 1 cut(s) 304
Bsp143I GATC 5 cut(s) 208, 328, 452, 557, 730
Bsp1720I GCTNAGC 1 cut(s) 1052
BspACI CCGC 9 cut(s) 180, 188, 203, 424, 563, 650, 671, 674, 802
BspANI GGCC 3 cut(s) 641, 695, 1016
BspCNI CTCAG 3 cut(s) 69, 121, 541
BspDI ATCGAT 1 cut(s) 249
BspEI TCCGGA 1 cut(s) 304
BspFNI CGCG 1 cut(s) 804
BspLI GGNNCC 6 cut(s) 422, 534, 598, 694, 732, 751
BspPI GGATC 3 cut(s) 460, 725, 738
BspQI GCTCTTC 1 cut(s) 1041
BspT107I GGYRCC 3 cut(s) 532, 596, 749
BsrBI CCGCTC 2 cut(s) 426, 671
BsrDI GCAATG 1 cut(s) 120
BsrFI RCCGGY 2 cut(s) 462, 752
BsrI ACTGG 1 cut(s) 183
BssAI RCCGGY 2 cut(s) 462, 752
BssECI CCNNGG 1 cut(s) 742
BssMI GATC 5 cut(s) 208, 328, 452, 557, 730
BssNAI GTATAC 1 cut(s) 928
BssNI GRCGYC 1 cut(s) 891
BssSI CACGAG 2 cut(s) 1025, 1071
Bst1107I GTATAC 1 cut(s) 928
Bst2BI CACGAG 2 cut(s) 1025, 1071
Bst2UI CCWGG 2 cut(s) 704, 743
Bst4CI ACNGT 5 cut(s) 126, 195, 238, 596, 1065
Bst6I CTCTTC 2 cut(s) 767, 1041
BstACI GRCGYC 1 cut(s) 891
BstAPI GCANNNNNTGC 1 cut(s) 119
BstC8I GCNNGC 2 cut(s) 120, 866
BstDEI CTNAG 7 cut(s) 9, 56, 108, 273, 528, 646, 1052
BstENI CCTNNNNNAGG 1 cut(s) 143
BstFNI CGCG 1 cut(s) 804
BstHHI GCGC 1 cut(s) 230
BstKTI GATC 5 cut(s) 211, 331, 455, 560, 733
BstMAI GTCTC 3 cut(s) 9, 43, 385
BstMBI GATC 5 cut(s) 208, 328, 452, 557, 730
BstMWI GCNNNNNNNGC 4 cut(s) 119, 361, 647, 671
BstNI CCWGG 2 cut(s) 704, 743
BstNSI RCATGY 1 cut(s) 122
BstSCI CCNGG 4 cut(s) 448, 702, 741, 846
BstUI CGCG 1 cut(s) 804
BstV1I GCAGC 3 cut(s) 274, 397, 830
BstV2I GAAGAC 1 cut(s) 614
BstX2I RGATCY 1 cut(s) 730
BstXI CCANNNNNNTGG 1 cut(s) 703
BstYI RGATCY 1 cut(s) 730
BstZ17I GTATAC 1 cut(s) 928
Bsu15I ATCGAT 1 cut(s) 249
BsuRI GGCC 3 cut(s) 641, 695, 1016
BsuTUI ATCGAT 1 cut(s) 249
Cac8I GCNNGC 2 cut(s) 120, 866
CfoI GCGC 1 cut(s) 230
Cfr10I RCCGGY 2 cut(s) 462, 752
Cfr13I GGNCC 2 cut(s) 126, 693
ClaI ATCGAT 1 cut(s) 249
CseI GACGC 2 cut(s) 791, 899
Csp6I GTAC 3 cut(s) 568, 720, 853
CviAII CATG 7 cut(s) 37, 61, 119, 130, 394, 697, 1097
CviQI GTAC 3 cut(s) 568, 720, 853
DdeI CTNAG 7 cut(s) 9, 56, 108, 273, 528, 646, 1052
DpnI GATC 5 cut(s) 210, 330, 454, 559, 732
DpnII GATC 5 cut(s) 208, 328, 452, 557, 730
DrdI GACNNNNNNGTC 1 cut(s) 797
DseDI GACNNNNNNGTC 1 cut(s) 797
EaeI YGGCCR 1 cut(s) 1014
Eam1104I CTCTTC 2 cut(s) 767, 1041
EarI CTCTTC 2 cut(s) 767, 1041
EciI GGCGGA 3 cut(s) 169, 177, 689
Eco47I GGWCC 1 cut(s) 126
Eco88I CYCGRG 1 cut(s) 44
EcoNI CCTNNNNNAGG 1 cut(s) 143
EcoRII CCWGG 2 cut(s) 702, 741
EcoT22I ATGCAT 2 cut(s) 120, 973
FaeI CATG 7 cut(s) 40, 64, 122, 133, 397, 700, 1100
FaqI GGGAC 1 cut(s) 803
FatI CATG 7 cut(s) 36, 60, 118, 129, 393, 696, 1096
FblI GTMKAC 2 cut(s) 757, 927
Fnu4HI GCNGC 5 cut(s) 288, 411, 564, 672, 819
Fsp4HI GCNGC 5 cut(s) 288, 411, 564, 672, 819
FspBI CTAG 3 cut(s) 143, 996, 1092
GlaI GCGC 1 cut(s) 229
GluI GCNGC 5 cut(s) 288, 411, 564, 672, 819
HaeIII GGCC 3 cut(s) 641, 695, 1016
HgaI GACGC 2 cut(s) 791, 899
HhaI GCGC 1 cut(s) 230
Hin1I GRCGYC 1 cut(s) 891
Hin1II CATG 7 cut(s) 40, 64, 122, 133, 397, 700, 1100
Hin6I GCGC 1 cut(s) 228
HinP1I GCGC 1 cut(s) 228
HincII GTYRAC 1 cut(s) 889
HindII GTYRAC 1 cut(s) 889
HindIII AAGCTT 1 cut(s) 862
HinfI GANTC 3 cut(s) 294, 837, 940
HphI GGTGA 4 cut(s) 128, 158, 964, 1126
Hpy166II GTNNAC 9 cut(s) 166, 198, 241, 312, 758, 853, 889, 928, 944
Hpy188I TCNGA 3 cut(s) 328, 976, 1008
Hpy188III TCNNGA 4 cut(s) 46, 305, 779, 1071
Hpy8I GTNNAC 9 cut(s) 166, 198, 241, 312, 758, 853, 889, 928, 944
Hpy99I CGWCG 1 cut(s) 419
HpyAV CCTTC 1 cut(s) 517
HpyCH4III ACNGT 5 cut(s) 126, 195, 238, 596, 1065
HpyCH4IV ACGT 1 cut(s) 21
HpyCH4V TGCA 8 cut(s) 118, 122, 393, 517, 868, 908, 971, 1123
HpyF10VI GCNNNNNNNGC 4 cut(s) 119, 361, 647, 671
HpyF3I CTNAG 7 cut(s) 9, 56, 108, 273, 528, 646, 1052
HpySE526I ACGT 1 cut(s) 21
Hsp92I GRCGYC 1 cut(s) 891
Hsp92II CATG 7 cut(s) 40, 64, 122, 133, 397, 700, 1100
HspAI GCGC 1 cut(s) 228
Kpn2I TCCGGA 1 cut(s) 304
Kzo9I GATC 5 cut(s) 208, 328, 452, 557, 730
LguI GCTCTTC 1 cut(s) 1041
LmnI GCTCC 1 cut(s) 431
Lsp1109I GCAGC 3 cut(s) 274, 397, 830
LweI GCATC 3 cut(s) 274, 958, 980
MaeI CTAG 3 cut(s) 143, 996, 1092
MaeII ACGT 1 cut(s) 21
MaeIII GTNAC 2 cut(s) 32, 544
MalI GATC 5 cut(s) 210, 330, 454, 559, 732
MbiI CCGCTC 2 cut(s) 426, 671
MboI GATC 5 cut(s) 208, 328, 452, 557, 730
MboII GAAGA 6 cut(s) 313, 359, 619, 754, 1058, 1120
MfeI CAATTG 1 cut(s) 909
MflI RGATCY 1 cut(s) 730
MhlI GDGCHC 1 cut(s) 1026
MluCI AATT 2 cut(s) 909, 1117
MlyI GAGTC 1 cut(s) 949
MmeI TCCRAC 1 cut(s) 986
Mph1103I ATGCAT 2 cut(s) 120, 973
MroI TCCGGA 1 cut(s) 304
MroXI GAANNNNTTC 1 cut(s) 162
MseI TTAA 2 cut(s) 681, 1159
MslI CAYNNNNRTG 1 cut(s) 392
MspR9I CCNGG 4 cut(s) 450, 704, 743, 848
MunI CAATTG 1 cut(s) 909
MvaI CCWGG 2 cut(s) 704, 743
MvnI CGCG 1 cut(s) 804
MwoI GCNNNNNNNGC 4 cut(s) 119, 361, 647, 671
NciI CCSGG 2 cut(s) 450, 848
NdeII GATC 5 cut(s) 208, 328, 452, 557, 730
NlaIII CATG 7 cut(s) 40, 64, 122, 133, 397, 700, 1100
NlaIV GGNNCC 6 cut(s) 422, 534, 598, 694, 732, 751
NmeAIII GCCGAG 1 cut(s) 1109
NmuCI GTSAC 1 cut(s) 544
NsiI ATGCAT 2 cut(s) 120, 973
NspI RCATGY 1 cut(s) 122
PaeI GCATGC 1 cut(s) 122
PaeR7I CTCGAG 1 cut(s) 44
PciSI GCTCTTC 1 cut(s) 1041
PcsI WCGNNNNNNNCGW 1 cut(s) 789
PdmI GAANNNNTTC 1 cut(s) 162
PfeI GAWTC 2 cut(s) 294, 837
PflFI GACNNNGTC 1 cut(s) 797
PfoI TCCNGGA 1 cut(s) 448
PkrI GCNGC 5 cut(s) 289, 412, 565, 673, 820
PleI GAGTC 1 cut(s) 948
PpsI GAGTC 1 cut(s) 948
Psp1406I AACGTT 1 cut(s) 21
Psp6I CCWGG 2 cut(s) 702, 741
PspGI CCWGG 2 cut(s) 702, 741
PspN4I GGNNCC 6 cut(s) 422, 534, 598, 694, 732, 751
PspPI GGNCC 2 cut(s) 126, 693
PsrI GAACNNNNNNTAC 2 cut(s) 413, 445
PsuI RGATCY 1 cut(s) 730
PsyI GACNNNGTC 1 cut(s) 797
RsaI GTAC 3 cut(s) 569, 721, 854
RsaNI GTAC 3 cut(s) 568, 720, 853
RseI CAYNNNNRTG 1 cut(s) 392
SapI GCTCTTC 1 cut(s) 1041
SaqAI TTAA 2 cut(s) 681, 1159
SatI GCNGC 5 cut(s) 288, 411, 564, 672, 819
Sau3AI GATC 5 cut(s) 208, 328, 452, 557, 730
Sau96I GGNCC 2 cut(s) 126, 693
SchI GAGTC 1 cut(s) 949
ScrFI CCNGG 4 cut(s) 450, 704, 743, 848
SduI GDGCHC 1 cut(s) 1026
SfaNI GCATC 3 cut(s) 274, 958, 980
Sfr274I CTCGAG 1 cut(s) 44
SinI GGWCC 1 cut(s) 126
SlaI CTCGAG 1 cut(s) 44
SmiMI CAYNNNNRTG 1 cut(s) 392
SmlI CTYRAG 2 cut(s) 44, 102
SmoI CTYRAG 2 cut(s) 44, 102
SphI GCATGC 1 cut(s) 122
Sse9I AATT 2 cut(s) 909, 1117
SsiI CCGC 9 cut(s) 180, 188, 203, 424, 563, 650, 671, 674, 802
SspMI CTAG 3 cut(s) 143, 996, 1092
StyD4I CCNGG 4 cut(s) 448, 702, 741, 846
TaaI ACNGT 5 cut(s) 126, 195, 238, 596, 1065
TaiI ACGT 1 cut(s) 24
TaqI TCGA 3 cut(s) 45, 249, 297
TasI AATT 2 cut(s) 909, 1117
TauI GCSGC 2 cut(s) 566, 674
TfiI GAWTC 2 cut(s) 294, 837
Tru1I TTAA 2 cut(s) 681, 1159
Tru9I TTAA 2 cut(s) 681, 1159
TseFI GTSAC 1 cut(s) 544
TseI GCWGC 3 cut(s) 287, 410, 818
Tsp45I GTSAC 1 cut(s) 544
TspDTI ATGAA 4 cut(s) 53, 77, 244, 577
TspGWI ACGGA 1 cut(s) 563
Tth111I GACNNNGTC 1 cut(s) 797
VpaK11BI GGWCC 1 cut(s) 126
XagI CCTNNNNNAGG 1 cut(s) 143
XapI RAATTY 1 cut(s) 1117
XceI RCATGY 1 cut(s) 122
XcmI CCANNNNNNNNNTGG 1 cut(s) 1108
XhoI CTCGAG 1 cut(s) 44
XmiI GTMKAC 2 cut(s) 757, 927
XmnI GAANNNNTTC 1 cut(s) 162
XspI CTAG 3 cut(s) 143, 996, 1092
Zsp2I ATGCAT 2 cut(s) 120, 973
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.