FvH4_3g23570

Indole-3-acetate O-methyltransferase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0008751
Physical Location & Seq
Reverse (-)
16648344 .. 16652946
4603 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_3g23570.t2

Sequence Viewer

Length: 1101 bp
ATGAAAGGAGGCAAAGGAGAAGCCAGCTATGCCAACAATTCCCAAGCCCAGGCCATACATGCAAGATCTATGCTTCATCTTCTGAAAGAAACCCTAGACAGTGTTCAGTTGAGTTCTCCCGATGTTCCCTTTGTGGTTGTGGACTTGGGATGCTCCAGCGGAAGCAACACCATTTACATCGTTGATGTGATCATCAAGCACATTACCAAGCGTTACGAGGCCTCCGGAATTGACCCGCCGGAGTTCTCTGCGTTTTTCTCCGACCTTCCTAGCAACGATTTCAACACTCTCTTCCAGCTCCTTCCTCCTCTGGCCAACTACGGAGCTGGGAGCATGGAGGAGTGCCTCGCCGCCGATAACCACCGCTCGTACTTTGCGGCAGGAGTTCCGGGTTCCTTTTACCGGAGGCTTTTCCCGTCCAGGTCCATTGACCTCTTTCACTCGGCGTTTTCCCTGCATTGGCTTTCTCAGGTGCCGGAGAGTGTGGTGGACAAGCGATCGGCGGCGTACAACAAAGGGAGGGTGTTCATCCACGGCGCGGAGGAGTGCACGGCCAAGGCTTATAAGAAGCAGTATCAGACAGACCTAGCAAGCTTCCTGCTAGCAAGATCAAAGGAGATGAAGAAAGGTGGCTCCATGTTCCTTGTTTGCCTCGGCAGGACCTCCGTCGACCCCACCGACCAGGGTGGTCCGGGCATTCTCTTCGGGACCCACTTCCAGGATGCCTGGGACGATCTTCTCCAAGAGGGTCTTATCACTAGTGAGAAACGTGACACTTTCAACATTCCGGTGTATGCATCAAGTTTGCAAGACTTCAAGGAGGTTGTAGAAGCGGACGGGTCATTCACCATTAACAAGCTTGAGATCTTCAAAGGAGGGAGCCCCCTTGTTGTGAACCAGCCCGATGATGCAGCCGAAGTAGGTCGAGCCCTCGCCAACAGCTGCCGGAGTGTGGCTGGAGTGCTCGTTGATGCACACATCGGCGAGAAACTCAGCAATGAACTATTCCTGCGAGTAGAAAAGAGAGGCGCATCACATGCCAAAGAGCTGCTAGAGAAACTACAATTCTCTCACATCGTGGCTTCACTCTCTTTTTTATAA

Protein Analysis

367

Amino Acids

40.05

Weight (kDa)

5.89

Isoelectric Point (pI)

48.06

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 62 - 383 1.9e-102 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 564, 1099
AccB1I GGYRCC 1 cut(s) 472
AccBSI CCGCTC 1 cut(s) 366
AccI GTMKAC 1 cut(s) 669
AccII CGCG 1 cut(s) 539
AccIII TCCGGA 1 cut(s) 224
AciI CCGC 8 cut(s) 159, 236, 351, 364, 377, 503, 539, 833
AcoI YGGCCR 2 cut(s) 312, 552
AdeI CACNNNGTG 1 cut(s) 1078
AfaI GTAC 2 cut(s) 371, 509
AfiI CCNNNNNNNGG 6 cut(s) 49, 402, 459, 538, 718, 952
AgsI TTSAA 4 cut(s) 283, 781, 817, 871
AhlI ACTAGT 1 cut(s) 758
AjnI CCWGG 5 cut(s) 48, 419, 681, 717, 725
AluBI AGCT 7 cut(s) 27, 298, 326, 594, 859, 942, 1048
AluI AGCT 7 cut(s) 27, 298, 326, 594, 859, 942, 1048
Alw21I GWGCWC 2 cut(s) 551, 966
Alw44I GTGCAC 1 cut(s) 547
Aor13HI TCCGGA 1 cut(s) 224
AoxI GGCC 4 cut(s) 51, 219, 312, 552
ApaLI GTGCAC 1 cut(s) 547
ApeKI GCWGC 3 cut(s) 911, 942, 1048
AspLEI GCGC 2 cut(s) 539, 1031
AspS9I GGNCC 4 cut(s) 423, 660, 689, 708
AsuC2I CCSGG 2 cut(s) 390, 693
AsuHPI GGTGA 1 cut(s) 838
AsuNHI GCTAGC 1 cut(s) 601
AvaII GGWCC 4 cut(s) 423, 660, 689, 708
BaeGI GKGCMC 1 cut(s) 551
BalI TGGCCA 1 cut(s) 314
BanI GGYRCC 1 cut(s) 472
BanII GRGCYC 2 cut(s) 884, 931
Bbv12I GWGCWC 2 cut(s) 551, 966
BbvI GCAGC 3 cut(s) 923, 929, 1035
BceAI ACGGC 2 cut(s) 550, 567
BcgI CGANNNNNNTGC 2 cut(s) 685, 719
BciT130I CCWGG 5 cut(s) 50, 421, 683, 719, 727
BclI TGATCA 1 cut(s) 189
BcnI CCSGG 2 cut(s) 390, 693
BcuI ACTAGT 1 cut(s) 758
BfaI CTAG 6 cut(s) 95, 270, 587, 602, 759, 1052
BglII AGATCT 2 cut(s) 65, 864
BisI GCNGC 6 cut(s) 351, 378, 504, 912, 943, 1049
BlsI GCNGC 6 cut(s) 352, 379, 505, 913, 944, 1050
Bme1390I CCNGG 7 cut(s) 50, 390, 421, 683, 693, 719, 727
Bme18I GGWCC 4 cut(s) 423, 660, 689, 708
BmgT120I GGNCC 4 cut(s) 423, 660, 689, 708
BmiI GGNNCC 6 cut(s) 394, 474, 634, 709, 710, 881
BmrFI CCNGG 7 cut(s) 50, 390, 421, 683, 693, 719, 727
BmsI GCATC 6 cut(s) 140, 712, 806, 898, 961, 1040
BmtI GCTAGC 1 cut(s) 605
BoxI GACNNNNGTC 1 cut(s) 665
BpmI CTGGAG 2 cut(s) 139, 978
BpuEI CTTGAG 1 cut(s) 881
BpuMI CCSGG 2 cut(s) 390, 693
BsaJI CCNNGG 6 cut(s) 48, 532, 555, 652, 682, 726
BsaWI WCCGGW 3 cut(s) 224, 402, 787
BsaXI ACNNNNNCTCC 4 cut(s) 206, 236, 951, 981
Bsc4I CCNNNNNNNGG 6 cut(s) 49, 402, 459, 538, 718, 952
Bse3DI GCAATG 1 cut(s) 1003
BseAI TCCGGA 1 cut(s) 224
BseBI CCWGG 5 cut(s) 50, 421, 683, 719, 727
BseDI CCNNGG 6 cut(s) 48, 532, 555, 652, 682, 726
BseGI GGATG 3 cut(s) 155, 528, 727
BseLI CCNNNNNNNGG 6 cut(s) 49, 402, 459, 538, 718, 952
BseMI GCAATG 1 cut(s) 1003
BseMII CTCAG 2 cut(s) 482, 1006
BseRI GAGGAG 3 cut(s) 297, 353, 557
BseSI GKGCMC 1 cut(s) 551
BseXI GCAGC 3 cut(s) 923, 929, 1035
BseYI CCCAGC 1 cut(s) 326
Bsh1236I CGCG 1 cut(s) 539
Bsh1285I CGRYCG 1 cut(s) 500
BshFI GGCC 4 cut(s) 53, 221, 314, 554
BshNI GGYRCC 1 cut(s) 472
BsiEI CGRYCG 1 cut(s) 500
BsiHKAI GWGCWC 2 cut(s) 551, 966
BsiSI CCGG 8 cut(s) 225, 239, 389, 403, 476, 692, 788, 946
BslFI GGGAC 2 cut(s) 721, 743
BslI CCNNNNNNNGG 6 cut(s) 49, 402, 459, 538, 718, 952
BsmFI GGGAC 2 cut(s) 721, 743
BsmI GAATGC 1 cut(s) 696
BsnI GGCC 4 cut(s) 53, 221, 314, 554
Bsp1286I GDGCHC 4 cut(s) 551, 884, 931, 966
Bsp13I TCCGGA 1 cut(s) 224
Bsp143I GATC 6 cut(s) 65, 189, 497, 608, 733, 864
BspACI CCGC 8 cut(s) 159, 236, 351, 364, 377, 503, 539, 833
BspANI GGCC 4 cut(s) 53, 221, 314, 554
BspCNI CTCAG 2 cut(s) 481, 1005
BspEI TCCGGA 1 cut(s) 224
BspFNI CGCG 1 cut(s) 539
BspLI GGNNCC 6 cut(s) 394, 474, 634, 709, 710, 881
BspOI GCTAGC 1 cut(s) 605
BspT107I GGYRCC 1 cut(s) 472
BsrBI CCGCTC 1 cut(s) 366
BsrDI GCAATG 1 cut(s) 1003
BssECI CCNNGG 6 cut(s) 48, 532, 555, 652, 682, 726
BssMI GATC 6 cut(s) 65, 189, 497, 608, 733, 864
BssT1I CCWWGG 1 cut(s) 555
Bst2UI CCWGG 5 cut(s) 50, 421, 683, 719, 727
Bst4CI ACNGT 1 cut(s) 101
Bst6I CTCTTC 2 cut(s) 296, 707
BstAPI GCANNNNNTGC 1 cut(s) 1037
BstC8I GCNNGC 3 cut(s) 25, 592, 603
BstDEI CTNAG 2 cut(s) 468, 992
BstDSI CCRYGG 1 cut(s) 532
BstF5I GGATG 3 cut(s) 155, 528, 727
BstFNI CGCG 1 cut(s) 539
BstHHI GCGC 2 cut(s) 539, 1031
BstKTI GATC 6 cut(s) 68, 192, 500, 611, 736, 867
BstMBI GATC 6 cut(s) 65, 189, 497, 608, 733, 864
BstMCI CGRYCG 1 cut(s) 500
BstMWI GCNNNNNNNGC 3 cut(s) 29, 59, 1037
BstNI CCWGG 5 cut(s) 50, 421, 683, 719, 727
BstNSI RCATGY 2 cut(s) 62, 1040
BstPAI GACNNNNGTC 1 cut(s) 665
BstSCI CCNGG 7 cut(s) 48, 388, 419, 681, 691, 717, 725
BstSLI GKGCMC 1 cut(s) 551
BstUI CGCG 1 cut(s) 539
BstV1I GCAGC 3 cut(s) 923, 929, 1035
BstX2I RGATCY 2 cut(s) 65, 864
BstYI RGATCY 2 cut(s) 65, 864
BsuRI GGCC 4 cut(s) 53, 221, 314, 554
BtgI CCRYGG 1 cut(s) 532
BtsCI GGATG 3 cut(s) 155, 528, 727
BtsIMutI CAGTG 1 cut(s) 106
Cac8I GCNNGC 3 cut(s) 25, 592, 603
CfoI GCGC 2 cut(s) 539, 1031
Cfr13I GGNCC 4 cut(s) 423, 660, 689, 708
Csp6I GTAC 2 cut(s) 370, 508
CviAII CATG 4 cut(s) 59, 334, 637, 1037
CviQI GTAC 2 cut(s) 370, 508
DdeI CTNAG 2 cut(s) 468, 992
DpnI GATC 6 cut(s) 67, 191, 499, 610, 735, 866
DpnII GATC 6 cut(s) 65, 189, 497, 608, 733, 864
DraIII CACNNNGTG 1 cut(s) 1078
EaeI YGGCCR 2 cut(s) 312, 552
Eam1104I CTCTTC 2 cut(s) 296, 707
EarI CTCTTC 2 cut(s) 296, 707
Eco130I CCWWGG 1 cut(s) 555
Eco147I AGGCCT 1 cut(s) 221
Eco24I GRGCYC 2 cut(s) 884, 931
Eco47I GGWCC 4 cut(s) 423, 660, 689, 708
EcoO109I RGGNCCY 2 cut(s) 660, 708
EcoRII CCWGG 5 cut(s) 48, 419, 681, 717, 725
EcoT14I CCWWGG 1 cut(s) 555
EcoT22I ATGCAT 1 cut(s) 799
EcoT38I GRGCYC 2 cut(s) 884, 931
ErhI CCWWGG 1 cut(s) 555
FaeI CATG 4 cut(s) 62, 337, 640, 1040
FalI AAGNNNNNCTT 2 cut(s) 735, 767
FaqI GGGAC 2 cut(s) 721, 743
FatI CATG 4 cut(s) 58, 333, 636, 1036
FauI CCCGC 1 cut(s) 243
FbaI TGATCA 1 cut(s) 189
FblI GTMKAC 1 cut(s) 669
Fnu4HI GCNGC 6 cut(s) 351, 378, 504, 912, 943, 1049
FokI GGATG 3 cut(s) 162, 515, 734
FriOI GRGCYC 2 cut(s) 884, 931
Fsp4HI GCNGC 6 cut(s) 351, 378, 504, 912, 943, 1049
FspBI CTAG 6 cut(s) 95, 270, 587, 602, 759, 1052
GlaI GCGC 2 cut(s) 538, 1030
GluI GCNGC 6 cut(s) 351, 378, 504, 912, 943, 1049
GsaI CCCAGC 1 cut(s) 330
GsuI CTGGAG 2 cut(s) 139, 978
HaeIII GGCC 4 cut(s) 53, 221, 314, 554
HapII CCGG 8 cut(s) 225, 239, 389, 403, 476, 692, 788, 946
HhaI GCGC 2 cut(s) 539, 1031
Hin1II CATG 4 cut(s) 62, 337, 640, 1040
Hin6I GCGC 2 cut(s) 537, 1029
HinP1I GCGC 2 cut(s) 537, 1029
HincII GTYRAC 1 cut(s) 670
HindII GTYRAC 1 cut(s) 670
HindIII AAGCTT 2 cut(s) 592, 857
HpaII CCGG 8 cut(s) 225, 239, 389, 403, 476, 692, 788, 946
HphI GGTGA 1 cut(s) 838
Hpy166II GTNNAC 5 cut(s) 142, 490, 549, 670, 895
Hpy188I TCNGA 3 cut(s) 84, 262, 579
Hpy188III TCNNGA 3 cut(s) 119, 225, 706
Hpy8I GTNNAC 5 cut(s) 142, 490, 549, 670, 895
Hpy99I CGWCG 1 cut(s) 671
HpyAV CCTTC 2 cut(s) 275, 311
HpyCH4III ACNGT 1 cut(s) 101
HpyCH4IV ACGT 1 cut(s) 769
HpyCH4V TGCA 7 cut(s) 62, 457, 549, 797, 808, 911, 974
HpyF10VI GCNNNNNNNGC 3 cut(s) 29, 59, 1037
HpyF3I CTNAG 2 cut(s) 468, 992
HpySE526I ACGT 1 cut(s) 769
Hsp92II CATG 4 cut(s) 62, 337, 640, 1040
HspAI GCGC 2 cut(s) 537, 1029
KflI GGGWCCC 1 cut(s) 708
Kpn2I TCCGGA 1 cut(s) 224
Ksp22I TGATCA 1 cut(s) 189
Kzo9I GATC 6 cut(s) 65, 189, 497, 608, 733, 864
LmnI GCTCC 6 cut(s) 158, 303, 323, 330, 638, 879
Lsp1109I GCAGC 3 cut(s) 923, 929, 1035
LweI GCATC 6 cut(s) 140, 712, 806, 898, 961, 1040
MaeI CTAG 6 cut(s) 95, 270, 587, 602, 759, 1052
MaeII ACGT 1 cut(s) 769
MaeIII GTNAC 2 cut(s) 212, 770
MalI GATC 6 cut(s) 67, 191, 499, 610, 735, 866
MbiI CCGCTC 1 cut(s) 366
MboI GATC 6 cut(s) 65, 189, 497, 608, 733, 864
MboII GAAGA 6 cut(s) 71, 283, 634, 694, 728, 859
MflI RGATCY 2 cut(s) 65, 864
MhlI GDGCHC 4 cut(s) 551, 884, 931, 966
MlsI TGGCCA 1 cut(s) 314
MluCI AATT 3 cut(s) 37, 228, 1064
MluNI TGGCCA 1 cut(s) 314
MmeI TCCRAC 1 cut(s) 285
Mox20I TGGCCA 1 cut(s) 314
Mph1103I ATGCAT 1 cut(s) 799
MroI TCCGGA 1 cut(s) 224
MscI TGGCCA 1 cut(s) 314
MseI TTAA 1 cut(s) 852
MslI CAYNNNNRTG 1 cut(s) 788
Msp20I TGGCCA 1 cut(s) 314
MspA1I CMGCKG 2 cut(s) 159, 942
MspI CCGG 8 cut(s) 225, 239, 389, 403, 476, 692, 788, 946
MspR9I CCNGG 7 cut(s) 50, 390, 421, 683, 693, 719, 727
Mva1269I GAATGC 1 cut(s) 696
MvaI CCWGG 5 cut(s) 50, 421, 683, 719, 727
MvnI CGCG 1 cut(s) 539
MwoI GCNNNNNNNGC 3 cut(s) 29, 59, 1037
NciI CCSGG 2 cut(s) 390, 693
NdeII GATC 6 cut(s) 65, 189, 497, 608, 733, 864
NheI GCTAGC 1 cut(s) 601
NlaIII CATG 4 cut(s) 62, 337, 640, 1040
NlaIV GGNNCC 6 cut(s) 394, 474, 634, 709, 710, 881
NmeAIII GCCGAG 2 cut(s) 422, 633
NmuCI GTSAC 1 cut(s) 770
NsiI ATGCAT 1 cut(s) 799
NspI RCATGY 2 cut(s) 62, 1040
PceI AGGCCT 1 cut(s) 221
PcsI WCGNNNNNNNCGW 1 cut(s) 675
PctI GAATGC 1 cut(s) 696
PfoI TCCNGGA 1 cut(s) 717
PkrI GCNGC 6 cut(s) 352, 379, 505, 913, 944, 1050
Ple19I CGATCG 1 cut(s) 500
PpuMI RGGWCCY 2 cut(s) 660, 708
PshAI GACNNNNGTC 1 cut(s) 665
PsiI TTATAA 2 cut(s) 564, 1099
Psp5II RGGWCCY 2 cut(s) 660, 708
Psp6I CCWGG 5 cut(s) 48, 419, 681, 717, 725
PspFI CCCAGC 1 cut(s) 326
PspGI CCWGG 5 cut(s) 48, 419, 681, 717, 725
PspN4I GGNNCC 6 cut(s) 394, 474, 634, 709, 710, 881
PspPI GGNCC 4 cut(s) 423, 660, 689, 708
PspPPI RGGWCCY 2 cut(s) 660, 708
PsuI RGATCY 2 cut(s) 65, 864
PvuI CGATCG 1 cut(s) 500
PvuII CAGCTG 1 cut(s) 942
RsaI GTAC 2 cut(s) 371, 509
RsaNI GTAC 2 cut(s) 370, 508
RseI CAYNNNNRTG 1 cut(s) 788
SalI GTCGAC 1 cut(s) 668
SaqAI TTAA 1 cut(s) 852
SatI GCNGC 6 cut(s) 351, 378, 504, 912, 943, 1049
Sau3AI GATC 6 cut(s) 65, 189, 497, 608, 733, 864
Sau96I GGNCC 4 cut(s) 423, 660, 689, 708
ScrFI CCNGG 7 cut(s) 50, 390, 421, 683, 693, 719, 727
SduI GDGCHC 4 cut(s) 551, 884, 931, 966
SfaNI GCATC 6 cut(s) 140, 712, 806, 898, 961, 1040
SinI GGWCC 4 cut(s) 423, 660, 689, 708
SmiMI CAYNNNNRTG 1 cut(s) 788
SmlI CTYRAG 1 cut(s) 860
SmoI CTYRAG 1 cut(s) 860
SpeI ACTAGT 1 cut(s) 758
Sse9I AATT 3 cut(s) 37, 228, 1064
SseBI AGGCCT 1 cut(s) 221
SsiI CCGC 8 cut(s) 159, 236, 351, 364, 377, 503, 539, 833
SspMI CTAG 6 cut(s) 95, 270, 587, 602, 759, 1052
StuI AGGCCT 1 cut(s) 221
StyD4I CCNGG 7 cut(s) 48, 388, 419, 681, 691, 717, 725
StyI CCWWGG 1 cut(s) 555
TaaI ACNGT 1 cut(s) 101
TaiI ACGT 1 cut(s) 772
TaqI TCGA 2 cut(s) 669, 925
TasI AATT 3 cut(s) 37, 228, 1064
TauI GCSGC 3 cut(s) 353, 380, 506
Tru1I TTAA 1 cut(s) 852
Tru9I TTAA 1 cut(s) 852
TscAI CASTG 1 cut(s) 106
TseFI GTSAC 1 cut(s) 770
TseI GCWGC 3 cut(s) 911, 942, 1048
Tsp45I GTSAC 1 cut(s) 770
TspDTI ATGAA 5 cut(s) 17, 65, 517, 635, 1014
TspGWI ACGGA 2 cut(s) 336, 655
TspRI CASTG 1 cut(s) 106
VneI GTGCAC 1 cut(s) 547
VpaK11BI GGWCC 4 cut(s) 423, 660, 689, 708
XceI RCATGY 2 cut(s) 62, 1040
XmiI GTMKAC 1 cut(s) 669
XspI CTAG 6 cut(s) 95, 270, 587, 602, 759, 1052
Zsp2I ATGCAT 1 cut(s) 799
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.