Rw5G026300

Indole-3-acetate O-methyltransferase 1-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_wichuraiana
Chr5
OG0008751
Physical Location & Seq
Reverse (-)
38014378 .. 38017739
3362 bp
Loading structure...
UTR
Exon/CDS
Intron
Rw5G026300.1

Sequence Viewer

Length: 1161 bp
ATGGCTCCAAAGGGAGACAATGTTGTAGTCTCTAACATAAAGCTTGAGAGGATTTTTAGCATGAAAGGAGGCAAAGGAGAAGCCAGCTATGCCAATAATTCCCAAGCGCAGGCCATACATGCAAGATCTATGCTTCACCTTCTCAAGGAAACCCTAGACAGTGTTCAGCTGAGCTCCCCCGATGTTCCCTTTGTGGTTGTGGACTTGGGGTGCTCGAGCGGAAGCAACACCATCTACATCGTCGATGTGATCATCAAGCACATTACCAAGCGCTACGAGGCCTCCGGGATTGACCCGCCGGAGTTCTCAGCGTTTTTCTCCGACCTCCCTAGCAATGATTTCAACACTCTCTTCCAGCTCCTCCCTCCTCTGGCCAACTACGGGGCCGGGAGCATGGAGGAGTGCCTCGCCGCAGATAACCACCGCTCGTACTTTGCGGCTGGGGTTCCGGGTTCCTTTTACCGGAGGCTTTTCCCGTCCAGGTCCATTGACCTCTTTCACTCGGCGTTTTCCTTGCATTGGCTTTCTCAGGTGCCGGAGAGTGTGGTGGACAAGCGATCGACGGCGTACAACAAAGGGAGGGTGTTCATCCACGGCGCGGAGGAGTGCACGGCCAAGGCTTACAAGAAGCAGTATCAAACAGACCTAGCGAGCTTCCTGCGAGCAAGATCAAAGGAGATGAAGAAAGGTGGCTCCATGTTCCTTGTTTGCCTCGGCAGGACCTCCGTCGACCCCACCGACCAGGGTGGTCCCGGCATTCTCTTCGGCACCCACTTTCAGGATGCCTGGGACGATCTTCTCCAAGAGGGGCTTATCACTAGTGACAAACGTGACACTTTCAACATTCCGGTGTATGCATCAAGTTTACAAGACTTTAAGGAGATTGTAGAAGCCGACGGCTCATTCACCATTAACAAGCTTGAGATTTTCAAAGGAGGGAGCCCCCTTGTGGTGAACCAGCCCGATGATGCAGCTGAAGTGGGTCGAGCCCTCGCCAACAGCTGCCGGAGTGTGGCTGGAGTCCTCGTCGATGCACACATCGGGGAGAAGCTCAGCGGTGAACTATTTTCACGAGTAGAAAAGAGAGGCACATCACATGCCAAAGAGCTGCTAGAGAAGCTACAATTCTATCACATAGTGGCTTCACTTTCTTTTTCATGA

Protein Analysis

386

Amino Acids

42.32

Weight (kDa)

6.14

Isoelectric Point (pI)

48.41

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Methyltransf_7 PF03492 62 - 383 1.3e-102 SAM dependent carboxyl methyltransferase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 532, 767
AccBSI CCGCTC 2 cut(s) 219, 426
AccI GTMKAC 1 cut(s) 729
AccII CGCG 1 cut(s) 599
AciI CCGC 7 cut(s) 219, 296, 411, 424, 437, 599, 1056
AcoI YGGCCR 2 cut(s) 372, 612
AcuI CTGAAG 1 cut(s) 996
AdeI CACNNNGTG 1 cut(s) 1138
AfaI GTAC 2 cut(s) 431, 569
AfeI AGCGCT 1 cut(s) 272
AgsI TTSAA 3 cut(s) 343, 841, 931
AhlI ACTAGT 1 cut(s) 818
AjnI CCWGG 3 cut(s) 479, 741, 785
Alw21I GWGCWC 3 cut(s) 176, 215, 611
Alw26I GTCTC 2 cut(s) 9, 34
Alw44I GTGCAC 1 cut(s) 607
Ama87I CYCGRG 1 cut(s) 214
Aor51HI AGCGCT 1 cut(s) 272
AoxI GGCC 5 cut(s) 111, 279, 372, 384, 612
ApaLI GTGCAC 1 cut(s) 607
ApeKI GCWGC 3 cut(s) 971, 1002, 1108
AspLEI GCGC 3 cut(s) 109, 273, 599
AspS9I GGNCC 4 cut(s) 384, 483, 720, 749
AsuC2I CCSGG 4 cut(s) 286, 388, 450, 753
AsuHPI GGTGA 4 cut(s) 128, 898, 964, 1070
AvaI CYCGRG 1 cut(s) 214
AvaII GGWCC 3 cut(s) 483, 720, 749
BaeGI GKGCMC 1 cut(s) 611
BalI TGGCCA 1 cut(s) 374
BanI GGYRCC 2 cut(s) 532, 767
BanII GRGCYC 3 cut(s) 176, 944, 991
BauI CACGAG 1 cut(s) 1071
Bbv12I GWGCWC 3 cut(s) 176, 215, 611
BbvI GCAGC 3 cut(s) 983, 989, 1095
BccI CCATC 1 cut(s) 239
BceAI ACGGC 4 cut(s) 579, 610, 627, 913
BcgI CGANNNNNNTGC 4 cut(s) 640, 674, 745, 779
BciT130I CCWGG 3 cut(s) 481, 743, 787
BclI TGATCA 1 cut(s) 249
BcnI CCSGG 4 cut(s) 286, 388, 450, 753
BcoDI GTCTC 2 cut(s) 9, 34
BcuI ACTAGT 1 cut(s) 818
BfaI CTAG 5 cut(s) 155, 330, 647, 819, 1112
BfoI RGCGCY 1 cut(s) 274
BglII AGATCT 1 cut(s) 125
BisI GCNGC 5 cut(s) 411, 438, 972, 1003, 1109
BlpI GCTNAGC 2 cut(s) 170, 1052
BlsI GCNGC 5 cut(s) 412, 439, 973, 1004, 1110
Bme1390I CCNGG 7 cut(s) 286, 388, 450, 481, 743, 753, 787
Bme18I GGWCC 3 cut(s) 483, 720, 749
BmeT110I CYCGRG 1 cut(s) 214
BmgT120I GGNCC 4 cut(s) 384, 483, 720, 749
BmiI GGNNCC 9 cut(s) 6, 385, 447, 454, 534, 694, 751, 769, 941
BmrFI CCNGG 7 cut(s) 286, 388, 450, 481, 743, 753, 787
BmsI GCATC 4 cut(s) 772, 866, 958, 1021
BoxI GACNNNNGTC 1 cut(s) 725
BpmI CTGGAG 1 cut(s) 1038
Bpu1102I GCTNAGC 2 cut(s) 170, 1052
BpuEI CTTGAG 3 cut(s) 65, 128, 941
BpuMI CCSGG 4 cut(s) 286, 388, 450, 753
BsaJI CCNNGG 5 cut(s) 592, 615, 712, 742, 786
BsaWI WCCGGW 2 cut(s) 462, 847
BsaXI ACNNNNNCTCC 6 cut(s) 6, 36, 266, 296, 1011, 1041
Bse3DI GCAATG 1 cut(s) 340
BseBI CCWGG 3 cut(s) 481, 743, 787
BseDI CCNNGG 5 cut(s) 592, 615, 712, 742, 786
BseGI GGATG 2 cut(s) 588, 787
BseMI GCAATG 1 cut(s) 340
BseMII CTCAG 4 cut(s) 161, 321, 542, 1066
BseRI GAGGAG 4 cut(s) 350, 357, 413, 617
BseSI GKGCMC 1 cut(s) 611
BseXI GCAGC 3 cut(s) 983, 989, 1095
BseYI CCCAGC 1 cut(s) 440
Bsh1236I CGCG 1 cut(s) 599
Bsh1285I CGRYCG 1 cut(s) 560
BshFI GGCC 5 cut(s) 113, 281, 374, 386, 614
BshNI GGYRCC 2 cut(s) 532, 767
BsiEI CGRYCG 1 cut(s) 560
BsiHKAI GWGCWC 3 cut(s) 176, 215, 611
BsiHKCI CYCGRG 1 cut(s) 214
BsiSI CCGG 9 cut(s) 285, 299, 387, 449, 463, 536, 753, 848, 1006
BslFI GGGAC 2 cut(s) 735, 803
BsmAI GTCTC 2 cut(s) 9, 34
BsmFI GGGAC 2 cut(s) 735, 803
BsmI GAATGC 1 cut(s) 756
BsnI GGCC 5 cut(s) 113, 281, 374, 386, 614
BsoBI CYCGRG 1 cut(s) 214
Bsp1286I GDGCHC 5 cut(s) 176, 215, 611, 944, 991
Bsp143I GATC 5 cut(s) 125, 249, 557, 668, 793
Bsp1720I GCTNAGC 2 cut(s) 170, 1052
BspACI CCGC 7 cut(s) 219, 296, 411, 424, 437, 599, 1056
BspANI GGCC 5 cut(s) 113, 281, 374, 386, 614
BspCNI CTCAG 4 cut(s) 162, 320, 541, 1065
BspFNI CGCG 1 cut(s) 599
BspHI TCATGA 1 cut(s) 1157
BspLI GGNNCC 9 cut(s) 6, 385, 447, 454, 534, 694, 751, 769, 941
BspT107I GGYRCC 2 cut(s) 532, 767
BsrBI CCGCTC 2 cut(s) 219, 426
BsrDI GCAATG 1 cut(s) 340
BssECI CCNNGG 5 cut(s) 592, 615, 712, 742, 786
BssMI GATC 5 cut(s) 125, 249, 557, 668, 793
BssSI CACGAG 1 cut(s) 1071
BssT1I CCWWGG 1 cut(s) 615
Bst2BI CACGAG 1 cut(s) 1071
Bst2UI CCWGG 3 cut(s) 481, 743, 787
Bst4CI ACNGT 1 cut(s) 161
Bst6I CTCTTC 2 cut(s) 356, 767
BstC8I GCNNGC 4 cut(s) 85, 111, 652, 663
BstDEI CTNAG 4 cut(s) 170, 307, 528, 1052
BstDSI CCRYGG 1 cut(s) 592
BstENI CCTNNNNNAGG 1 cut(s) 143
BstF5I GGATG 2 cut(s) 588, 787
BstFNI CGCG 1 cut(s) 599
BstH2I RGCGCY 1 cut(s) 274
BstHHI GCGC 3 cut(s) 109, 273, 599
BstKTI GATC 5 cut(s) 128, 252, 560, 671, 796
BstMAI GTCTC 2 cut(s) 9, 34
BstMBI GATC 5 cut(s) 125, 249, 557, 668, 793
BstMCI CGRYCG 1 cut(s) 560
BstMWI GCNNNNNNNGC 3 cut(s) 89, 119, 1117
BstNI CCWGG 3 cut(s) 481, 743, 787
BstNSI RCATGY 2 cut(s) 122, 1100
BstPAI GACNNNNGTC 1 cut(s) 725
BstSCI CCNGG 7 cut(s) 284, 386, 448, 479, 741, 751, 785
BstSLI GKGCMC 1 cut(s) 611
BstUI CGCG 1 cut(s) 599
BstV1I GCAGC 3 cut(s) 983, 989, 1095
BstX2I RGATCY 1 cut(s) 125
BstYI RGATCY 1 cut(s) 125
BsuRI GGCC 5 cut(s) 113, 281, 374, 386, 614
BtgI CCRYGG 1 cut(s) 592
BtsCI GGATG 2 cut(s) 588, 787
BtsIMutI CAGTG 1 cut(s) 166
Cac8I GCNNGC 4 cut(s) 85, 111, 652, 663
CciI TCATGA 1 cut(s) 1157
CfoI GCGC 3 cut(s) 109, 273, 599
Cfr13I GGNCC 4 cut(s) 384, 483, 720, 749
Csp6I GTAC 2 cut(s) 430, 568
CviAII CATG 6 cut(s) 61, 119, 394, 697, 1097, 1158
CviQI GTAC 2 cut(s) 430, 568
DdeI CTNAG 4 cut(s) 170, 307, 528, 1052
DpnI GATC 5 cut(s) 127, 251, 559, 670, 795
DpnII GATC 5 cut(s) 125, 249, 557, 668, 793
DraIII CACNNNGTG 1 cut(s) 1138
EaeI YGGCCR 2 cut(s) 372, 612
Eam1104I CTCTTC 2 cut(s) 356, 767
EarI CTCTTC 2 cut(s) 356, 767
Ecl136II GAGCTC 1 cut(s) 174
Eco130I CCWWGG 1 cut(s) 615
Eco147I AGGCCT 1 cut(s) 281
Eco24I GRGCYC 3 cut(s) 176, 944, 991
Eco47I GGWCC 3 cut(s) 483, 720, 749
Eco47III AGCGCT 1 cut(s) 272
Eco53kI GAGCTC 1 cut(s) 174
Eco57I CTGAAG 1 cut(s) 996
Eco88I CYCGRG 1 cut(s) 214
EcoICRI GAGCTC 1 cut(s) 174
EcoNI CCTNNNNNAGG 1 cut(s) 143
EcoO109I RGGNCCY 1 cut(s) 720
EcoRII CCWGG 3 cut(s) 479, 741, 785
EcoT14I CCWWGG 1 cut(s) 615
EcoT22I ATGCAT 1 cut(s) 859
EcoT38I GRGCYC 3 cut(s) 176, 944, 991
ErhI CCWWGG 1 cut(s) 615
FaeI CATG 6 cut(s) 64, 122, 397, 700, 1100, 1161
FalI AAGNNNNNCTT 2 cut(s) 795, 827
FaqI GGGAC 2 cut(s) 735, 803
FatI CATG 6 cut(s) 60, 118, 393, 696, 1096, 1157
FauI CCCGC 1 cut(s) 303
FbaI TGATCA 1 cut(s) 249
FblI GTMKAC 1 cut(s) 729
Fnu4HI GCNGC 5 cut(s) 411, 438, 972, 1003, 1109
FokI GGATG 2 cut(s) 575, 794
FriOI GRGCYC 3 cut(s) 176, 944, 991
Fsp4HI GCNGC 5 cut(s) 411, 438, 972, 1003, 1109
FspBI CTAG 5 cut(s) 155, 330, 647, 819, 1112
GlaI GCGC 3 cut(s) 108, 272, 598
GluI GCNGC 5 cut(s) 411, 438, 972, 1003, 1109
GsaI CCCAGC 1 cut(s) 444
GsuI CTGGAG 1 cut(s) 1038
HaeII RGCGCY 1 cut(s) 274
HaeIII GGCC 5 cut(s) 113, 281, 374, 386, 614
HapII CCGG 9 cut(s) 285, 299, 387, 449, 463, 536, 753, 848, 1006
HhaI GCGC 3 cut(s) 109, 273, 599
Hin1II CATG 6 cut(s) 64, 122, 397, 700, 1100, 1161
Hin6I GCGC 3 cut(s) 107, 271, 597
HinP1I GCGC 3 cut(s) 107, 271, 597
HincII GTYRAC 1 cut(s) 730
HindII GTYRAC 1 cut(s) 730
HindIII AAGCTT 2 cut(s) 41, 917
HinfI GANTC 1 cut(s) 1020
HpaII CCGG 9 cut(s) 285, 299, 387, 449, 463, 536, 753, 848, 1006
HphI GGTGA 4 cut(s) 128, 898, 964, 1070
Hpy166II GTNNAC 7 cut(s) 202, 550, 609, 730, 866, 955, 1061
Hpy188I TCNGA 1 cut(s) 322
Hpy188III TCNNGA 3 cut(s) 779, 1071, 1158
Hpy8I GTNNAC 7 cut(s) 202, 550, 609, 730, 866, 955, 1061
Hpy99I CGWCG 5 cut(s) 245, 565, 731, 899, 1031
HpyAV CCTTC 1 cut(s) 149
HpyCH4III ACNGT 1 cut(s) 161
HpyCH4IV ACGT 1 cut(s) 829
HpyCH4V TGCA 6 cut(s) 122, 517, 609, 857, 971, 1034
HpyF10VI GCNNNNNNNGC 3 cut(s) 89, 119, 1117
HpyF3I CTNAG 4 cut(s) 170, 307, 528, 1052
HpySE526I ACGT 1 cut(s) 829
Hsp92II CATG 6 cut(s) 64, 122, 397, 700, 1100, 1161
HspAI GCGC 3 cut(s) 107, 271, 597
Ksp22I TGATCA 1 cut(s) 249
Kzo9I GATC 5 cut(s) 125, 249, 557, 668, 793
LmnI GCTCC 6 cut(s) 10, 179, 363, 390, 698, 939
Lsp1109I GCAGC 3 cut(s) 983, 989, 1095
LweI GCATC 4 cut(s) 772, 866, 958, 1021
MaeI CTAG 5 cut(s) 155, 330, 647, 819, 1112
MaeII ACGT 1 cut(s) 829
MaeIII GTNAC 2 cut(s) 821, 830
MalI GATC 5 cut(s) 127, 251, 559, 670, 795
MbiI CCGCTC 2 cut(s) 219, 426
MboI GATC 5 cut(s) 125, 249, 557, 668, 793
MboII GAAGA 4 cut(s) 343, 694, 754, 788
MflI RGATCY 1 cut(s) 125
MhlI GDGCHC 5 cut(s) 176, 215, 611, 944, 991
MlsI TGGCCA 1 cut(s) 374
MluCI AATT 2 cut(s) 97, 1124
MluNI TGGCCA 1 cut(s) 374
MlyI GAGTC 1 cut(s) 1029
MmeI TCCRAC 1 cut(s) 345
Mox20I TGGCCA 1 cut(s) 374
Mph1103I ATGCAT 1 cut(s) 859
MscI TGGCCA 1 cut(s) 374
MseI TTAA 2 cut(s) 876, 912
MslI CAYNNNNRTG 1 cut(s) 848
Msp20I TGGCCA 1 cut(s) 374
MspA1I CMGCKG 4 cut(s) 169, 974, 1002, 1056
MspI CCGG 9 cut(s) 285, 299, 387, 449, 463, 536, 753, 848, 1006
MspR9I CCNGG 7 cut(s) 286, 388, 450, 481, 743, 753, 787
Mva1269I GAATGC 1 cut(s) 756
MvaI CCWGG 3 cut(s) 481, 743, 787
MvnI CGCG 1 cut(s) 599
MwoI GCNNNNNNNGC 3 cut(s) 89, 119, 1117
NciI CCSGG 4 cut(s) 286, 388, 450, 753
NdeII GATC 5 cut(s) 125, 249, 557, 668, 793
NlaIII CATG 6 cut(s) 64, 122, 397, 700, 1100, 1161
NlaIV GGNNCC 9 cut(s) 6, 385, 447, 454, 534, 694, 751, 769, 941
NmeAIII GCCGAG 2 cut(s) 482, 693
NmuCI GTSAC 2 cut(s) 821, 830
NsiI ATGCAT 1 cut(s) 859
NspI RCATGY 2 cut(s) 122, 1100
PaeR7I CTCGAG 1 cut(s) 214
PagI TCATGA 1 cut(s) 1157
PceI AGGCCT 1 cut(s) 281
PcsI WCGNNNNNNNCGW 1 cut(s) 735
PctI GAATGC 1 cut(s) 756
PfoI TCCNGGA 1 cut(s) 284
PkrI GCNGC 5 cut(s) 412, 439, 973, 1004, 1110
Ple19I CGATCG 1 cut(s) 560
PleI GAGTC 1 cut(s) 1028
PpsI GAGTC 1 cut(s) 1028
PpuMI RGGWCCY 1 cut(s) 720
PshAI GACNNNNGTC 1 cut(s) 725
Psp124BI GAGCTC 1 cut(s) 176
Psp5II RGGWCCY 1 cut(s) 720
Psp6I CCWGG 3 cut(s) 479, 741, 785
PspFI CCCAGC 1 cut(s) 440
PspGI CCWGG 3 cut(s) 479, 741, 785
PspN4I GGNNCC 9 cut(s) 6, 385, 447, 454, 534, 694, 751, 769, 941
PspPI GGNCC 4 cut(s) 384, 483, 720, 749
PspPPI RGGWCCY 1 cut(s) 720
PspXI VCTCGAGB 1 cut(s) 214
PsuI RGATCY 1 cut(s) 125
PvuI CGATCG 1 cut(s) 560
PvuII CAGCTG 3 cut(s) 169, 974, 1002
RsaI GTAC 2 cut(s) 431, 569
RsaNI GTAC 2 cut(s) 430, 568
RseI CAYNNNNRTG 1 cut(s) 848
SacI GAGCTC 1 cut(s) 176
SalI GTCGAC 1 cut(s) 728
SaqAI TTAA 2 cut(s) 876, 912
SatI GCNGC 5 cut(s) 411, 438, 972, 1003, 1109
Sau3AI GATC 5 cut(s) 125, 249, 557, 668, 793
Sau96I GGNCC 4 cut(s) 384, 483, 720, 749
SchI GAGTC 1 cut(s) 1029
ScrFI CCNGG 7 cut(s) 286, 388, 450, 481, 743, 753, 787
SduI GDGCHC 5 cut(s) 176, 215, 611, 944, 991
SfaNI GCATC 4 cut(s) 772, 866, 958, 1021
Sfr274I CTCGAG 1 cut(s) 214
SinI GGWCC 3 cut(s) 483, 720, 749
SlaI CTCGAG 1 cut(s) 214
SmiMI CAYNNNNRTG 1 cut(s) 848
SmlI CTYRAG 4 cut(s) 44, 143, 214, 920
SmoI CTYRAG 4 cut(s) 44, 143, 214, 920
SpeI ACTAGT 1 cut(s) 818
Sse9I AATT 2 cut(s) 97, 1124
SseBI AGGCCT 1 cut(s) 281
SsiI CCGC 7 cut(s) 219, 296, 411, 424, 437, 599, 1056
SspMI CTAG 5 cut(s) 155, 330, 647, 819, 1112
SstI GAGCTC 1 cut(s) 176
StuI AGGCCT 1 cut(s) 281
StyD4I CCNGG 7 cut(s) 284, 386, 448, 479, 741, 751, 785
StyI CCWWGG 1 cut(s) 615
TaaI ACNGT 1 cut(s) 161
TaiI ACGT 1 cut(s) 832
TaqI TCGA 6 cut(s) 215, 243, 560, 729, 985, 1029
TasI AATT 2 cut(s) 97, 1124
TauI GCSGC 2 cut(s) 413, 440
Tru1I TTAA 2 cut(s) 876, 912
Tru9I TTAA 2 cut(s) 876, 912
TscAI CASTG 1 cut(s) 166
TseFI GTSAC 2 cut(s) 821, 830
TseI GCWGC 3 cut(s) 971, 1002, 1108
Tsp45I GTSAC 2 cut(s) 821, 830
TspDTI ATGAA 4 cut(s) 77, 577, 695, 1146
TspGWI ACGGA 1 cut(s) 715
TspRI CASTG 1 cut(s) 166
VneI GTGCAC 1 cut(s) 607
VpaK11BI GGWCC 3 cut(s) 483, 720, 749
XagI CCTNNNNNAGG 1 cut(s) 143
XceI RCATGY 2 cut(s) 122, 1100
XhoI CTCGAG 1 cut(s) 214
XmiI GTMKAC 1 cut(s) 729
XspI CTAG 5 cut(s) 155, 330, 647, 819, 1112
Zsp2I ATGCAT 1 cut(s) 859
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.