AT5G56130

Tho complex subunit

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0008473
Physical Location & Seq
Reverse (-)
22722482 .. 22725099
2618 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G56130.1

Sequence Viewer

Length: 948 bp
ATGGAGGAGACGACGATTCCTTTCAAGAGCTTACATAGCAGAGAATATCAAGGTCATAAGAAGAAGGTACATTCAGTAGCTTGGAATTCGAATGGAACAAAGCTTGCTTCGGGTTCTGTTGATCAAACGGCTCGCATTTGGAATATCGAACCACACGGTCATAGCAAGGCTAAGGACCTTGAGCTAAAGGGTCATACTGATAGTGTGGATCAGCTATGTTGGGATCCCAAACATTCAGATCTTGTTGCTACTGCCTCTGGTGATAAAAGCGTTCGTCTTTGGGATGCTCGCAGTGGAAAATGCACACAGCAGGTAGAACTTAGTGGAGAGAATATAAACATCACCTACAAACCTGACGGGACACATGTAGCTGTTGGTAATAGGGATGATGAGCTTACAATTCTGGATGTCCGTAAGTTTAAACCTCTACACAGGCGCAAATTCAATTATGAGGTTAATGAGATTGCTTGGAACATGCCTGGAGATTTTTTCTTCTTGACTACTGGACTTGGCACTGTTGAAGTTCTTTCATATCCATCTCTTAAACCACTTGACACTCTCACAGCCCACACAGCCGGGTGTTATTGTATTGCAATTGATCCTAAAGGGAGATATTTTGCTGTCGGGAGTGCGGATTCCTTAGTAAGCCTTTGGGATATCTCAGACATGCTTTGTTTGAGAACATTTACCAAACTTGAGTGGCCAGTCAGAACAATAAGCTTCAACTATTCGGGAGAGTATATTGCTTCAGCCAGTGAAGATTTATTCATTGATATTGCTAATGTCCAAACCGGGCGGACGGTGCATCAGATTCCATGTCGGGCAGCCATGAACAGTGTTGAGTGGAATCCCAAATACAATTTACTTGCATATGCAGGTGATGACAAGAATCCCAAGTATAATACTGATGAAGGTGTTTTCCGGATATTTGGTTTTGAGAGCTCGTAG

Protein Analysis

315

Amino Acids

35.37

Weight (kDa)

6.13

Isoelectric Point (pI)

24.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
WD40_CDC20-Fz PF24807 12 - 108 3.3e-17 CDC20/Fizzy WD40 domain
Beta-prop_WDR36-Utp21_1st PF25171 13 - 157 1.8e-06 WDR36/Utp21 first beta-propeller
WD40_Gbeta PF25391 13 - 140 9.3e-10 G protein beta WD-40 repeat protein
WD40_WDHD1_1st PF24817 13 - 158 3e-15 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 14 - 106 2.2e-18 WDR5 beta-propeller domain
Beta-prop_TEP1_2nd PF25047 14 - 104 1.4e-07 TEP-1 second beta-propeller
WD40_Prp19 PF24814 14 - 141 2.3e-20 Prp19 WD40 domain
WD40 PF00400 14 - 48 1.8e-09 WD domain, G-beta repeat
WDR55 PF24796 15 - 140 6.7e-10 WDR55
Beta-prop_WDR3_1st PF25173 15 - 135 1.8e-20 WDR3 first beta-propeller domain
Beta-prop_CAF1B_HIR1 PF24105 15 - 107 3.9e-15 CAF1B/HIR1 beta-propeller domain
Beta-prop_IFT122_1st PF23381 16 - 129 2.2e-06 IFT122 first beta-propeller
Beta-prop_THOC3 PF25174 19 - 292 1.2e-122 THOC3 beta-propeller domain
Beta-prop_Aladin PF25460 46 - 167 3.5e-06 Aladin seven-bladed propeller
WD40 PF00400 61 - 95 3e-06 WD domain, G-beta repeat
Beta-prop_WDR36-Utp21_2nd PF25168 63 - 214 1.7e-06 WDR36/Utp21 second beta-propeller domain
Beta-prop_WDR19_1st PF23389 72 - 220 5.4e-09 WDR19 first beta-propeller
Beta-prop_EML_2 PF23414 73 - 146 2e-10 Echinoderm microtubule-associated protein second beta-propeller
WD40_WDHD1_1st PF24817 113 - 292 1.7e-19 WDHD1 first WD40 domain
Beta-prop_WDR5 PF25175 114 - 287 2.3e-22 WDR5 beta-propeller domain
WD40_CDC20-Fz PF24807 114 - 291 8.4e-18 CDC20/Fizzy WD40 domain
Beta-prop_EML_2 PF23414 129 - 286 7.3e-15 Echinoderm microtubule-associated protein second beta-propeller
EIF3I PF24805 142 - 252 3e-09 EIF3I
WD40_Prp19 PF24814 150 - 295 9.2e-15 Prp19 WD40 domain
Beta-prop_WDR3_1st PF25173 151 - 296 4.2e-12 WDR3 first beta-propeller domain
WD40 PF00400 182 - 219 2.9e-08 WD domain, G-beta repeat
WD40_Gbeta PF25391 186 - 259 1.7e-08 G protein beta WD-40 repeat protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AarI CACCTGC 1 cut(s) 866
Acc36I ACCTGC 2 cut(s) 301, 866
AccIII TCCGGA 1 cut(s) 921
AciI CCGC 2 cut(s) 632, 796
AclWI GGATC 4 cut(s) 216, 218, 231, 593
AcoI YGGCCR 1 cut(s) 701
AcsI RAATTY 2 cut(s) 85, 440
AcuI CTGAAG 1 cut(s) 732
AfaI GTAC 1 cut(s) 69
AflIII ACRYGT 1 cut(s) 364
AgsI TTSAA 4 cut(s) 25, 445, 521, 724
AjnI CCWGG 1 cut(s) 478
AluBI AGCT 9 cut(s) 30, 80, 103, 184, 214, 371, 394, 720, 942
AluI AGCT 9 cut(s) 30, 80, 103, 184, 214, 371, 394, 720, 942
Alw21I GWGCWC 1 cut(s) 944
Alw26I GTCTC 1 cut(s) 2
AlwI GGATC 4 cut(s) 216, 218, 231, 593
Aor13HI TCCGGA 1 cut(s) 921
AoxI GGCC 1 cut(s) 701
ApeKI GCWGC 1 cut(s) 824
ApoI RAATTY 2 cut(s) 85, 440
AspLEI GCGC 1 cut(s) 438
AspS9I GGNCC 1 cut(s) 175
AsuC2I CCSGG 2 cut(s) 577, 793
AsuHPI GGTGA 3 cut(s) 272, 334, 890
AsuII TTCGAA 1 cut(s) 89
AvaII GGWCC 1 cut(s) 175
BalI TGGCCA 1 cut(s) 703
BamHI GGATCC 1 cut(s) 223
BanII GRGCYC 1 cut(s) 944
Bbv12I GWGCWC 1 cut(s) 944
BbvI GCAGC 1 cut(s) 836
BccI CCATC 1 cut(s) 544
BceAI ACGGC 1 cut(s) 144
BciT130I CCWGG 1 cut(s) 480
BclI TGATCA 1 cut(s) 121
BcnI CCSGG 2 cut(s) 577, 793
BcoDI GTCTC 1 cut(s) 2
BfuAI ACCTGC 2 cut(s) 301, 866
BglII AGATCT 1 cut(s) 238
BisI GCNGC 1 cut(s) 825
BlsI GCNGC 1 cut(s) 826
Bme1390I CCNGG 3 cut(s) 480, 577, 793
Bme18I GGWCC 1 cut(s) 175
BmgT120I GGNCC 1 cut(s) 175
BmiI GGNNCC 1 cut(s) 225
BmrFI CCNGG 3 cut(s) 480, 577, 793
BmsI GCATC 2 cut(s) 274, 814
BpmI CTGGAG 1 cut(s) 501
Bpu10I CCTNAGC 1 cut(s) 171
Bpu14I TTCGAA 1 cut(s) 89
BpuEI CTTGAG 2 cut(s) 200, 716
BpuMI CCSGG 2 cut(s) 577, 793
BsaWI WCCGGW 1 cut(s) 921
Bse1I ACTGG 3 cut(s) 508, 704, 753
BseAI TCCGGA 1 cut(s) 921
BseBI CCWGG 1 cut(s) 480
BseGI GGATG 3 cut(s) 289, 391, 412
BseMII CTCAG 1 cut(s) 675
BseNI ACTGG 3 cut(s) 508, 704, 753
BseRI GAGGAG 1 cut(s) 20
BseXI GCAGC 1 cut(s) 836
BshFI GGCC 1 cut(s) 703
BsiHKAI GWGCWC 1 cut(s) 944
BsiSI CCGG 3 cut(s) 576, 792, 922
BslFI GGGAC 1 cut(s) 373
BsmAI GTCTC 1 cut(s) 2
BsmBI CGTCTC 1 cut(s) 2
BsmFI GGGAC 1 cut(s) 373
BsnI GGCC 1 cut(s) 703
Bsp119I TTCGAA 1 cut(s) 89
Bsp1286I GDGCHC 1 cut(s) 944
Bsp13I TCCGGA 1 cut(s) 921
Bsp143I GATC 5 cut(s) 121, 208, 223, 238, 598
BspACI CCGC 2 cut(s) 632, 796
BspANI GGCC 1 cut(s) 703
BspCNI CTCAG 1 cut(s) 674
BspEI TCCGGA 1 cut(s) 921
BspLI GGNNCC 1 cut(s) 225
BspMI ACCTGC 2 cut(s) 301, 866
BspPI GGATC 4 cut(s) 216, 218, 231, 593
BspT104I TTCGAA 1 cut(s) 89
BsrI ACTGG 3 cut(s) 508, 704, 753
BssMI GATC 5 cut(s) 121, 208, 223, 238, 598
Bst2UI CCWGG 1 cut(s) 480
Bst4CI ACNGT 4 cut(s) 158, 517, 802, 836
BstBI TTCGAA 1 cut(s) 89
BstC8I GCNNGC 3 cut(s) 105, 133, 289
BstDEI CTNAG 4 cut(s) 171, 320, 640, 661
BstF5I GGATG 3 cut(s) 289, 391, 412
BstHHI GCGC 1 cut(s) 438
BstKTI GATC 5 cut(s) 124, 211, 226, 241, 601
BstMAI GTCTC 1 cut(s) 2
BstMBI GATC 5 cut(s) 121, 208, 223, 238, 598
BstMWI GCNNNNNNNGC 3 cut(s) 36, 572, 802
BstNI CCWGG 1 cut(s) 480
BstNSI RCATGY 3 cut(s) 368, 478, 670
BstSCI CCNGG 3 cut(s) 478, 575, 791
BstV1I GCAGC 1 cut(s) 836
BstX2I RGATCY 2 cut(s) 223, 238
BstYI RGATCY 2 cut(s) 223, 238
BsuRI GGCC 1 cut(s) 703
BtsCI GGATG 3 cut(s) 289, 391, 412
BtsI GCAGTG 1 cut(s) 298
BtsIMutI CAGTG 4 cut(s) 298, 513, 760, 841
BveI ACCTGC 2 cut(s) 301, 866
Cac8I GCNNGC 3 cut(s) 105, 133, 289
CfoI GCGC 1 cut(s) 438
Cfr13I GGNCC 1 cut(s) 175
Csp6I GTAC 1 cut(s) 68
CviAII CATG 5 cut(s) 365, 475, 667, 816, 829
CviQI GTAC 1 cut(s) 68
DdeI CTNAG 4 cut(s) 171, 320, 640, 661
DpnI GATC 5 cut(s) 123, 210, 225, 240, 600
DpnII GATC 5 cut(s) 121, 208, 223, 238, 598
DraI TTTAAA 1 cut(s) 421
EaeI YGGCCR 1 cut(s) 701
EciI GGCGGA 1 cut(s) 811
Ecl136II GAGCTC 1 cut(s) 942
Eco24I GRGCYC 1 cut(s) 944
Eco32I GATATC 1 cut(s) 658
Eco47I GGWCC 1 cut(s) 175
Eco53kI GAGCTC 1 cut(s) 942
Eco57I CTGAAG 1 cut(s) 732
EcoICRI GAGCTC 1 cut(s) 942
EcoO109I RGGNCCY 1 cut(s) 175
EcoRI GAATTC 1 cut(s) 85
EcoRII CCWGG 1 cut(s) 478
EcoRV GATATC 1 cut(s) 658
EcoT38I GRGCYC 1 cut(s) 944
Esp3I CGTCTC 1 cut(s) 2
FaeI CATG 5 cut(s) 368, 478, 670, 819, 832
FaqI GGGAC 1 cut(s) 373
FatI CATG 5 cut(s) 364, 474, 666, 815, 828
FauNDI CATATG 1 cut(s) 871
FbaI TGATCA 1 cut(s) 121
Fnu4HI GCNGC 1 cut(s) 825
FokI GGATG 3 cut(s) 296, 398, 419
FriOI GRGCYC 1 cut(s) 944
Fsp4HI GCNGC 1 cut(s) 825
GlaI GCGC 1 cut(s) 437
GluI GCNGC 1 cut(s) 825
GsuI CTGGAG 1 cut(s) 501
HaeIII GGCC 1 cut(s) 703
HapII CCGG 3 cut(s) 576, 792, 922
HhaI GCGC 1 cut(s) 438
Hin1II CATG 5 cut(s) 368, 478, 670, 819, 832
Hin6I GCGC 1 cut(s) 436
HinP1I GCGC 1 cut(s) 436
HindIII AAGCTT 2 cut(s) 101, 718
HinfI GANTC 5 cut(s) 16, 635, 811, 847, 889
HpaII CCGG 3 cut(s) 576, 792, 922
HphI GGTGA 3 cut(s) 272, 334, 890
Hpy188I TCNGA 4 cut(s) 238, 664, 710, 810
Hpy188III TCNNGA 6 cut(s) 25, 404, 496, 625, 732, 922
Hpy99I CGWCG 1 cut(s) 16
HpyAV CCTTC 2 cut(s) 58, 905
HpyCH4III ACNGT 4 cut(s) 158, 517, 802, 836
HpyCH4V TGCA 5 cut(s) 303, 593, 805, 869, 875
HpyF10VI GCNNNNNNNGC 3 cut(s) 36, 572, 802
HpyF3I CTNAG 4 cut(s) 171, 320, 640, 661
Hsp92II CATG 5 cut(s) 368, 478, 670, 819, 832
HspAI GCGC 1 cut(s) 436
Kpn2I TCCGGA 1 cut(s) 921
Ksp22I TGATCA 1 cut(s) 121
Kzo9I GATC 5 cut(s) 121, 208, 223, 238, 598
Lsp1109I GCAGC 1 cut(s) 836
LweI GCATC 2 cut(s) 274, 814
MalI GATC 5 cut(s) 123, 210, 225, 240, 600
MboI GATC 5 cut(s) 121, 208, 223, 238, 598
MboII GAAGA 3 cut(s) 73, 484, 770
MfeI CAATTG 1 cut(s) 594
MflI RGATCY 2 cut(s) 223, 238
MhlI GDGCHC 1 cut(s) 944
MlsI TGGCCA 1 cut(s) 703
MluCI AATT 6 cut(s) 85, 399, 440, 445, 594, 859
MluNI TGGCCA 1 cut(s) 703
MnlI CCTC 3 cut(s) 265, 435, 445
Mox20I TGGCCA 1 cut(s) 703
MroI TCCGGA 1 cut(s) 921
MscI TGGCCA 1 cut(s) 703
MseI TTAA 3 cut(s) 420, 456, 543
Msp20I TGGCCA 1 cut(s) 703
MspI CCGG 3 cut(s) 576, 792, 922
MspR9I CCNGG 3 cut(s) 480, 577, 793
MssI GTTTAAAC 1 cut(s) 421
MunI CAATTG 1 cut(s) 594
MvaI CCWGG 1 cut(s) 480
MwoI GCNNNNNNNGC 3 cut(s) 36, 572, 802
NciI CCSGG 2 cut(s) 577, 793
NdeI CATATG 1 cut(s) 871
NdeII GATC 5 cut(s) 121, 208, 223, 238, 598
NlaIII CATG 5 cut(s) 368, 478, 670, 819, 832
NlaIV GGNNCC 1 cut(s) 225
NspI RCATGY 3 cut(s) 368, 478, 670
NspV TTCGAA 1 cut(s) 89
PaqCI CACCTGC 1 cut(s) 866
PciI ACATGT 1 cut(s) 364
PfeI GAWTC 5 cut(s) 16, 635, 811, 847, 889
PkrI GCNGC 1 cut(s) 826
PmeI GTTTAAAC 1 cut(s) 421
PpuMI RGGWCCY 1 cut(s) 175
PscI ACATGT 1 cut(s) 364
Psp124BI GAGCTC 1 cut(s) 944
Psp5II RGGWCCY 1 cut(s) 175
Psp6I CCWGG 1 cut(s) 478
PspGI CCWGG 1 cut(s) 478
PspN4I GGNNCC 1 cut(s) 225
PspPI GGNCC 1 cut(s) 175
PspPPI RGGWCCY 1 cut(s) 175
PsuI RGATCY 2 cut(s) 223, 238
RsaI GTAC 1 cut(s) 69
RsaNI GTAC 1 cut(s) 68
SacI GAGCTC 1 cut(s) 944
SaqAI TTAA 3 cut(s) 420, 456, 543
SatI GCNGC 1 cut(s) 825
Sau3AI GATC 5 cut(s) 121, 208, 223, 238, 598
Sau96I GGNCC 1 cut(s) 175
ScrFI CCNGG 3 cut(s) 480, 577, 793
SduI GDGCHC 1 cut(s) 944
SfaNI GCATC 2 cut(s) 274, 814
SfuI TTCGAA 1 cut(s) 89
SinI GGWCC 1 cut(s) 175
SmlI CTYRAG 2 cut(s) 179, 695
SmoI CTYRAG 2 cut(s) 179, 695
Sse9I AATT 6 cut(s) 85, 399, 440, 445, 594, 859
SsiI CCGC 2 cut(s) 632, 796
SstI GAGCTC 1 cut(s) 944
StyD4I CCNGG 3 cut(s) 478, 575, 791
TaaI ACNGT 4 cut(s) 158, 517, 802, 836
TaqI TCGA 2 cut(s) 89, 147
TasI AATT 6 cut(s) 85, 399, 440, 445, 594, 859
TfiI GAWTC 5 cut(s) 16, 635, 811, 847, 889
Tru1I TTAA 3 cut(s) 420, 456, 543
Tru9I TTAA 3 cut(s) 420, 456, 543
TscAI CASTG 4 cut(s) 298, 520, 760, 841
TseI GCWGC 1 cut(s) 824
TspDTI ATGAA 4 cut(s) 519, 757, 845, 924
TspGWI ACGGA 1 cut(s) 401
TspRI CASTG 4 cut(s) 298, 520, 760, 841
VpaK11BI GGWCC 1 cut(s) 175
XapI RAATTY 2 cut(s) 85, 440
XceI RCATGY 3 cut(s) 368, 478, 670
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.