AT5G57230

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010331
Physical Location & Seq
Forward (+)
23189938 .. 23191714
1777 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G57230.2

Sequence Viewer

Length: 483 bp
ATGGGAGATTCAACATTTCTCGATAGAATGCTTCTCCAGCTTCGCTCCACTTGCAAGTATTACAGCGGTTACCCTAAGGATCTCGGGCCATCGCGGGTTCTCCATTTTACATCAGAAAGAGAGTTTGTCCAACTCCTTCACCAGGGCTATCCTGTGGTTGTCGCATTCACCATAAGGAGTAACTACACACAACATCTTGACCGGATGCTTGAGGAAGCTGCTGCTGAGTTCTATCCCAACATAAAATTTATGCGTGTTGAGTGTCCCAAGTATCCCGGATTCTGCATAACTCGCCAGAAAAATGAATATCCTTTCATTGAAATATTCCATAGCCCACAACATGCCGGTAATGAAGGAAAAGTACAGGATCCAAATATCACTCGGTATTCAGTGAAAGTTGTACCTTACAATTATGATATGAGTCCCTACGGATTCAGAGAATTCTTTAAGCGCCAGGGGGTTCGTACTTCGGATCCCAAATGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

160

Amino Acids

18.84

Weight (kDa)

8.93

Isoelectric Point (pI)

57.64

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccII CGCG 1 cut(s) 94
AciI CCGC 2 cut(s) 66, 94
AclWI GGATC 5 cut(s) 87, 362, 375, 467, 480
AcsI RAATTY 2 cut(s) 245, 440
AfaI GTAC 3 cut(s) 363, 402, 466
AfiI CCNNNNNNNGG 1 cut(s) 142
AgsI TTSAA 2 cut(s) 12, 320
AjnI CCWGG 2 cut(s) 141, 453
AluBI AGCT 2 cut(s) 40, 218
AluI AGCT 2 cut(s) 40, 218
AlwI GGATC 5 cut(s) 87, 362, 375, 467, 480
Ama87I CYCGRG 1 cut(s) 83
AoxI GGCC 1 cut(s) 86
ApeKI GCWGC 2 cut(s) 218, 221
ApoI RAATTY 2 cut(s) 245, 440
AspLEI GCGC 1 cut(s) 453
AspS9I GGNCC 1 cut(s) 86
AsuC2I CCSGG 1 cut(s) 276
AsuHPI GGTGA 2 cut(s) 131, 160
AvaI CYCGRG 1 cut(s) 83
AxyI CCTNAGG 1 cut(s) 75
BamHI GGATCC 2 cut(s) 367, 472
BarI GAAGNNNNNNTAC 2 cut(s) 345, 377
BbvI GCAGC 2 cut(s) 205, 208
BccI CCATC 1 cut(s) 97
BciT130I CCWGG 2 cut(s) 143, 455
BciVI GTATCC 1 cut(s) 282
BcnI CCSGG 1 cut(s) 276
BfoI RGCGCY 1 cut(s) 454
BfuI GTATCC 1 cut(s) 282
BisI GCNGC 2 cut(s) 219, 222
BlsI GCNGC 2 cut(s) 220, 223
Bme1390I CCNGG 3 cut(s) 143, 276, 455
BmeT110I CYCGRG 1 cut(s) 83
BmgT120I GGNCC 1 cut(s) 86
BmiI GGNNCC 2 cut(s) 369, 474
BmrFI CCNGG 3 cut(s) 143, 276, 455
BmsI GCATC 1 cut(s) 195
BpmI CTGGAG 1 cut(s) 20
BpuEI CTTGAG 1 cut(s) 230
BpuMI CCSGG 1 cut(s) 276
BsaJI CCNNGG 2 cut(s) 142, 454
BsaWI WCCGGW 1 cut(s) 201
Bsc4I CCNNNNNNNGG 1 cut(s) 142
Bse118I RCCGGY 1 cut(s) 344
Bse21I CCTNAGG 1 cut(s) 75
BseBI CCWGG 2 cut(s) 143, 455
BseDI CCNNGG 2 cut(s) 142, 454
BseGI GGATG 1 cut(s) 210
BseLI CCNNNNNNNGG 1 cut(s) 142
BseMII CTCAG 1 cut(s) 216
BseXI GCAGC 2 cut(s) 205, 208
Bsh1236I CGCG 1 cut(s) 94
BshFI GGCC 1 cut(s) 88
BsiHKCI CYCGRG 1 cut(s) 83
BsiSI CCGG 3 cut(s) 202, 276, 345
BslFI GGGAC 2 cut(s) 249, 408
BslI CCNNNNNNNGG 1 cut(s) 142
BsmFI GGGAC 2 cut(s) 249, 408
BsmI GAATGC 2 cut(s) 33, 164
BsnI GGCC 1 cut(s) 88
BsoBI CYCGRG 1 cut(s) 83
Bsp143I GATC 3 cut(s) 79, 367, 472
BspACI CCGC 2 cut(s) 66, 94
BspANI GGCC 1 cut(s) 88
BspCNI CTCAG 1 cut(s) 217
BspFNI CGCG 1 cut(s) 94
BspLI GGNNCC 2 cut(s) 369, 474
BspPI GGATC 5 cut(s) 87, 362, 375, 467, 480
BsrFI RCCGGY 1 cut(s) 344
BssAI RCCGGY 1 cut(s) 344
BssECI CCNNGG 2 cut(s) 142, 454
BssMI GATC 3 cut(s) 79, 367, 472
Bst2UI CCWGG 2 cut(s) 143, 455
BstDEI CTNAG 2 cut(s) 75, 225
BstEII GGTNACC 1 cut(s) 68
BstENI CCTNNNNNAGG 1 cut(s) 140
BstF5I GGATG 1 cut(s) 210
BstFNI CGCG 1 cut(s) 94
BstH2I RGCGCY 1 cut(s) 454
BstHHI GCGC 1 cut(s) 453
BstKTI GATC 3 cut(s) 82, 370, 475
BstMBI GATC 3 cut(s) 79, 367, 472
BstMWI GCNNNNNNNGC 3 cut(s) 37, 51, 291
BstNI CCWGG 2 cut(s) 143, 455
BstNSI RCATGY 1 cut(s) 344
BstPI GGTNACC 1 cut(s) 68
BstSCI CCNGG 3 cut(s) 141, 274, 453
BstUI CGCG 1 cut(s) 94
BstV1I GCAGC 2 cut(s) 205, 208
BstX2I RGATCY 3 cut(s) 79, 367, 472
BstYI RGATCY 3 cut(s) 79, 367, 472
Bsu36I CCTNAGG 1 cut(s) 75
BsuI GTATCC 1 cut(s) 282
BsuRI GGCC 1 cut(s) 88
BtgZI GCGATG 1 cut(s) 75
BtsCI GGATG 1 cut(s) 210
BtsIMutI CAGTG 1 cut(s) 396
CfoI GCGC 1 cut(s) 453
Cfr10I RCCGGY 1 cut(s) 344
Cfr13I GGNCC 1 cut(s) 86
Csp6I GTAC 3 cut(s) 362, 401, 465
CviAII CATG 1 cut(s) 341
CviJI RGCY 5 cut(s) 40, 88, 147, 218, 333
CviKI_1 RGCY 5 cut(s) 40, 88, 147, 218, 333
CviQI GTAC 3 cut(s) 362, 401, 465
DdeI CTNAG 2 cut(s) 75, 225
DpnI GATC 3 cut(s) 81, 369, 474
DpnII GATC 3 cut(s) 79, 367, 472
Eco81I CCTNAGG 1 cut(s) 75
Eco88I CYCGRG 1 cut(s) 83
Eco91I GGTNACC 1 cut(s) 68
EcoNI CCTNNNNNAGG 1 cut(s) 140
EcoO65I GGTNACC 1 cut(s) 68
EcoRI GAATTC 1 cut(s) 440
EcoRII CCWGG 2 cut(s) 141, 453
FaeI CATG 1 cut(s) 344
FaiI YATR 8 cut(s) 173, 242, 251, 287, 330, 342, 414, 419
FaqI GGGAC 2 cut(s) 249, 408
FatI CATG 1 cut(s) 340
FauI CCCGC 1 cut(s) 87
Fnu4HI GCNGC 2 cut(s) 219, 222
FokI GGATG 1 cut(s) 217
Fsp4HI GCNGC 2 cut(s) 219, 222
GlaI GCGC 1 cut(s) 452
GluI GCNGC 2 cut(s) 219, 222
GsuI CTGGAG 1 cut(s) 20
HaeII RGCGCY 1 cut(s) 454
HaeIII GGCC 1 cut(s) 88
HapII CCGG 3 cut(s) 202, 276, 345
HhaI GCGC 1 cut(s) 453
Hin1II CATG 1 cut(s) 344
Hin6I GCGC 1 cut(s) 451
HinP1I GCGC 1 cut(s) 451
HinfI GANTC 4 cut(s) 8, 279, 421, 432
HpaII CCGG 3 cut(s) 202, 276, 345
HphI GGTGA 2 cut(s) 131, 160
Hpy188I TCNGA 3 cut(s) 115, 437, 472
Hpy188III TCNNGA 2 cut(s) 20, 197
HpyAV CCTTC 2 cut(s) 146, 347
HpyCH4V TGCA 2 cut(s) 54, 285
HpyF10VI GCNNNNNNNGC 3 cut(s) 37, 51, 291
HpyF3I CTNAG 2 cut(s) 75, 225
Hsp92II CATG 1 cut(s) 344
HspAI GCGC 1 cut(s) 451
Kzo9I GATC 3 cut(s) 79, 367, 472
LmnI GCTCC 1 cut(s) 50
Lsp1109I GCAGC 2 cut(s) 205, 208
LweI GCATC 1 cut(s) 195
MaeIII GTNAC 2 cut(s) 68, 179
MalI GATC 3 cut(s) 81, 369, 474
MboI GATC 3 cut(s) 79, 367, 472
MflI RGATCY 3 cut(s) 79, 367, 472
MluCI AATT 3 cut(s) 245, 409, 440
MlyI GAGTC 1 cut(s) 430
MmeI TCCRAC 1 cut(s) 154
MnlI CCTC 1 cut(s) 205
MseI TTAA 1 cut(s) 447
MspA1I CMGCKG 1 cut(s) 66
MspI CCGG 3 cut(s) 202, 276, 345
MspR9I CCNGG 3 cut(s) 143, 276, 455
Mva1269I GAATGC 2 cut(s) 33, 164
MvaI CCWGG 2 cut(s) 143, 455
MvnI CGCG 1 cut(s) 94
MwoI GCNNNNNNNGC 3 cut(s) 37, 51, 291
NciI CCSGG 1 cut(s) 276
NdeII GATC 3 cut(s) 79, 367, 472
NlaIII CATG 1 cut(s) 344
NlaIV GGNNCC 2 cut(s) 369, 474
NspI RCATGY 1 cut(s) 344
PctI GAATGC 2 cut(s) 33, 164
PfeI GAWTC 3 cut(s) 8, 279, 432
PfoI TCCNGGA 1 cut(s) 274
PkrI GCNGC 2 cut(s) 220, 223
PleI GAGTC 1 cut(s) 429
PpsI GAGTC 1 cut(s) 429
Psp6I CCWGG 2 cut(s) 141, 453
PspEI GGTNACC 1 cut(s) 68
PspGI CCWGG 2 cut(s) 141, 453
PspN4I GGNNCC 2 cut(s) 369, 474
PspPI GGNCC 1 cut(s) 86
PsuI RGATCY 3 cut(s) 79, 367, 472
RsaI GTAC 3 cut(s) 363, 402, 466
RsaNI GTAC 3 cut(s) 362, 401, 465
SaqAI TTAA 1 cut(s) 447
SatI GCNGC 2 cut(s) 219, 222
Sau3AI GATC 3 cut(s) 79, 367, 472
Sau96I GGNCC 1 cut(s) 86
SchI GAGTC 1 cut(s) 430
ScrFI CCNGG 3 cut(s) 143, 276, 455
SetI ASST 3 cut(s) 42, 220, 406
SfaNI GCATC 1 cut(s) 195
SmlI CTYRAG 1 cut(s) 209
SmoI CTYRAG 1 cut(s) 209
Sse9I AATT 3 cut(s) 245, 409, 440
SsiI CCGC 2 cut(s) 66, 94
SspI AATATT 1 cut(s) 324
StyD4I CCNGG 3 cut(s) 141, 274, 453
TaqI TCGA 1 cut(s) 21
TasI AATT 3 cut(s) 245, 409, 440
TatI WGTACW 1 cut(s) 361
TfiI GAWTC 3 cut(s) 8, 279, 432
Tru1I TTAA 1 cut(s) 447
Tru9I TTAA 1 cut(s) 447
TscAI CASTG 1 cut(s) 396
TseI GCWGC 2 cut(s) 218, 221
TspDTI ATGAA 3 cut(s) 304, 318, 366
TspGWI ACGGA 1 cut(s) 444
TspRI CASTG 1 cut(s) 396
XagI CCTNNNNNAGG 1 cut(s) 140
XapI RAATTY 2 cut(s) 245, 440
XceI RCATGY 1 cut(s) 344
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.