MD16G1249800.v1.1

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr16
OG0010331
Physical Location & Seq
Forward (+)
27952134 .. 27954697
2564 bp
Loading structure...
UTR
Exon/CDS
Intron
MD16G1249800.v1.1.491

Sequence Viewer

Length: 441 bp
ATGATCAGCCATCTACGTGCAACATCCAAGTATTATACTGGCTATCCAAAGGATCTTGGGCCATCAAGGGTCATCCATTTTACATCAGAACGCGAGTTTGTCCAGCTCCTTCATGAAGGCCATCCCGTGGTTGTTGCATTCACCCTCAGGTGTAATCTCACAAAACATCTTGATGGAGTATTGGAGGAAGCTGCAGCTGAGTTTTATCCCCATGTAAAATTTATGCGCGTCGAATGCCCAAAATATCCTGGTTTCTGTATAACACGGCAGAGGAAGGAATATCCATTTATAGAAATATTTCATAGTCCACAACAAGGAAGGGTTGCTGATGCAAATATTACAAAGTACTCTGTGAAAGTTGTTCCCTTCAATTACGACACTAGTGCCTACGGATTTAGAGAGTTTTTCAAGCGTCACGGGATACAGTCAAGTCCACAGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

147

Amino Acids

16.98

Weight (kDa)

9.15

Isoelectric Point (pI)

55.15

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 127
AccII CGCG 2 cut(s) 93, 228
AclWI GGATC 1 cut(s) 60
AcsI RAATTY 1 cut(s) 218
AfaI GTAC 1 cut(s) 347
AfiI CCNNNNNNNGG 2 cut(s) 127, 314
AgsI TTSAA 2 cut(s) 370, 409
AhlI ACTAGT 1 cut(s) 380
AjnI CCWGG 1 cut(s) 247
AluBI AGCT 3 cut(s) 106, 191, 197
AluI AGCT 3 cut(s) 106, 191, 197
AlwI GGATC 1 cut(s) 60
AoxI GGCC 2 cut(s) 59, 118
ApeKI GCWGC 2 cut(s) 191, 194
ApoI RAATTY 1 cut(s) 218
Asp700I GAANNNNTTC 1 cut(s) 297
AspLEI GCGC 1 cut(s) 228
AspS9I GGNCC 1 cut(s) 59
AsuHPI GGTGA 1 cut(s) 133
AxyI CCTNAGG 1 cut(s) 146
BbvI GCAGC 2 cut(s) 178, 206
BccI CCATC 4 cut(s) 18, 70, 129, 167
BceAI ACGGC 1 cut(s) 281
BcgI CGANNNNNNTGC 2 cut(s) 365, 399
BciT130I CCWGG 1 cut(s) 249
BciVI GTATCC 1 cut(s) 414
BclI TGATCA 1 cut(s) 3
BcuI ACTAGT 1 cut(s) 380
BfaI CTAG 1 cut(s) 381
BfmI CTRYAG 1 cut(s) 192
BfuI GTATCC 1 cut(s) 414
BisI GCNGC 2 cut(s) 192, 195
BlsI GCNGC 2 cut(s) 193, 196
BmcAI AGTACT 1 cut(s) 347
Bme1390I CCNGG 1 cut(s) 249
BmgT120I GGNCC 1 cut(s) 59
BmrFI CCNGG 1 cut(s) 249
BmsI GCATC 1 cut(s) 319
BsaAI YACGTR 1 cut(s) 17
BsaJI CCNNGG 1 cut(s) 126
Bsc4I CCNNNNNNNGG 2 cut(s) 127, 314
Bse1I ACTGG 1 cut(s) 43
Bse21I CCTNAGG 1 cut(s) 146
BseBI CCWGG 1 cut(s) 249
BseDI CCNNGG 1 cut(s) 126
BseGI GGATG 3 cut(s) 23, 72, 121
BseLI CCNNNNNNNGG 2 cut(s) 127, 314
BseMII CTCAG 2 cut(s) 160, 189
BseNI ACTGG 1 cut(s) 43
BseXI GCAGC 2 cut(s) 178, 206
Bsh1236I CGCG 2 cut(s) 93, 228
BshFI GGCC 2 cut(s) 61, 120
BslI CCNNNNNNNGG 2 cut(s) 127, 314
BsmI GAATGC 2 cut(s) 137, 239
BsnI GGCC 2 cut(s) 61, 120
Bsp143I GATC 2 cut(s) 3, 52
BspANI GGCC 2 cut(s) 61, 120
BspCNI CTCAG 2 cut(s) 159, 190
BspFNI CGCG 2 cut(s) 93, 228
BspHI TCATGA 1 cut(s) 112
BspMAI CTGCAG 1 cut(s) 196
BspPI GGATC 1 cut(s) 60
BsrI ACTGG 1 cut(s) 43
BssECI CCNNGG 1 cut(s) 126
BssMI GATC 2 cut(s) 3, 52
Bst2UI CCWGG 1 cut(s) 249
Bst4CI ACNGT 2 cut(s) 426, 438
BstBAI YACGTR 1 cut(s) 17
BstDEI CTNAG 2 cut(s) 146, 198
BstDSI CCRYGG 1 cut(s) 126
BstF5I GGATG 3 cut(s) 23, 72, 121
BstFNI CGCG 2 cut(s) 93, 228
BstHHI GCGC 1 cut(s) 228
BstKTI GATC 2 cut(s) 6, 55
BstMBI GATC 2 cut(s) 3, 52
BstMWI GCNNNNNNNGC 1 cut(s) 234
BstNI CCWGG 1 cut(s) 249
BstSCI CCNGG 1 cut(s) 247
BstSFI CTRYAG 1 cut(s) 192
BstUI CGCG 2 cut(s) 93, 228
BstV1I GCAGC 2 cut(s) 178, 206
BstX2I RGATCY 1 cut(s) 52
BstYI RGATCY 1 cut(s) 52
Bsu36I CCTNAGG 1 cut(s) 146
BsuI GTATCC 1 cut(s) 414
BsuRI GGCC 2 cut(s) 61, 120
BtgI CCRYGG 1 cut(s) 126
BtsCI GGATG 3 cut(s) 23, 72, 121
CciI TCATGA 1 cut(s) 112
CfoI GCGC 1 cut(s) 228
Cfr13I GGNCC 1 cut(s) 59
CseI GACGC 2 cut(s) 217, 401
Csp6I GTAC 1 cut(s) 346
CviAII CATG 2 cut(s) 113, 212
CviJI RGCY 7 cut(s) 9, 42, 61, 106, 120, 191, 197
CviKI_1 RGCY 7 cut(s) 9, 42, 61, 106, 120, 191, 197
CviQI GTAC 1 cut(s) 346
DdeI CTNAG 2 cut(s) 146, 198
DpnI GATC 2 cut(s) 5, 54
DpnII GATC 2 cut(s) 3, 52
Eco81I CCTNAGG 1 cut(s) 146
EcoRII CCWGG 1 cut(s) 247
FaeI CATG 2 cut(s) 116, 215
FaiI YATR 7 cut(s) 36, 114, 213, 224, 260, 290, 303
FatI CATG 2 cut(s) 112, 211
FbaI TGATCA 1 cut(s) 3
Fnu4HI GCNGC 2 cut(s) 192, 195
FokI GGATG 3 cut(s) 10, 59, 108
Fsp4HI GCNGC 2 cut(s) 192, 195
FspBI CTAG 1 cut(s) 381
GlaI GCGC 1 cut(s) 227
GluI GCNGC 2 cut(s) 192, 195
HaeIII GGCC 2 cut(s) 61, 120
HgaI GACGC 2 cut(s) 217, 401
HhaI GCGC 1 cut(s) 228
Hin1II CATG 2 cut(s) 116, 215
Hin6I GCGC 1 cut(s) 226
HinP1I GCGC 1 cut(s) 226
HphI GGTGA 1 cut(s) 133
Hpy166II GTNNAC 2 cut(s) 308, 434
Hpy188I TCNGA 1 cut(s) 88
Hpy188III TCNNGA 2 cut(s) 113, 170
Hpy8I GTNNAC 2 cut(s) 308, 434
Hpy99I CGWCG 1 cut(s) 233
HpyAV CCTTC 5 cut(s) 110, 119, 268, 312, 376
HpyCH4III ACNGT 2 cut(s) 426, 438
HpyCH4IV ACGT 1 cut(s) 16
HpyCH4V TGCA 4 cut(s) 20, 137, 194, 332
HpyF10VI GCNNNNNNNGC 1 cut(s) 234
HpyF3I CTNAG 2 cut(s) 146, 198
HpySE526I ACGT 1 cut(s) 16
Hsp92II CATG 2 cut(s) 116, 215
HspAI GCGC 1 cut(s) 226
Ksp22I TGATCA 1 cut(s) 3
Kzo9I GATC 2 cut(s) 3, 52
LmnI GCTCC 1 cut(s) 111
LpnPI CCDG 5 cut(s) 24, 116, 133, 234, 261
Lsp1109I GCAGC 2 cut(s) 178, 206
LweI GCATC 1 cut(s) 319
MaeI CTAG 1 cut(s) 381
MaeII ACGT 1 cut(s) 16
MaeIII GTNAC 1 cut(s) 413
MalI GATC 2 cut(s) 5, 54
MboI GATC 2 cut(s) 3, 52
MflI RGATCY 1 cut(s) 52
MluCI AATT 2 cut(s) 218, 370
MnlI CCTC 3 cut(s) 155, 178, 264
MroXI GAANNNNTTC 1 cut(s) 297
MslI CAYNNNNRTG 2 cut(s) 15, 171
MspA1I CMGCKG 1 cut(s) 197
MspR9I CCNGG 1 cut(s) 249
Mva1269I GAATGC 2 cut(s) 137, 239
MvaI CCWGG 1 cut(s) 249
MvnI CGCG 2 cut(s) 93, 228
MwoI GCNNNNNNNGC 1 cut(s) 234
NdeII GATC 2 cut(s) 3, 52
NlaIII CATG 2 cut(s) 116, 215
NmuCI GTSAC 1 cut(s) 413
PagI TCATGA 1 cut(s) 112
PctI GAATGC 2 cut(s) 137, 239
PdmI GAANNNNTTC 1 cut(s) 297
PflMI CCANNNNNTGG 1 cut(s) 127
PkrI GCNGC 2 cut(s) 193, 196
Ppu21I YACGTR 1 cut(s) 17
Psp6I CCWGG 1 cut(s) 247
PspGI CCWGG 1 cut(s) 247
PspPI GGNCC 1 cut(s) 59
PstI CTGCAG 1 cut(s) 196
PsuI RGATCY 1 cut(s) 52
PvuII CAGCTG 1 cut(s) 197
RsaI GTAC 1 cut(s) 347
RsaNI GTAC 1 cut(s) 346
RseI CAYNNNNRTG 2 cut(s) 15, 171
SatI GCNGC 2 cut(s) 192, 195
Sau3AI GATC 2 cut(s) 3, 52
Sau96I GGNCC 1 cut(s) 59
ScaI AGTACT 1 cut(s) 347
ScrFI CCNGG 1 cut(s) 249
SetI ASST 5 cut(s) 19, 108, 152, 193, 199
SfaNI GCATC 1 cut(s) 319
SfcI CTRYAG 1 cut(s) 192
SmiMI CAYNNNNRTG 2 cut(s) 15, 171
SpeI ACTAGT 1 cut(s) 380
Sse9I AATT 2 cut(s) 218, 370
SspI AATATT 2 cut(s) 297, 337
SspMI CTAG 1 cut(s) 381
StyD4I CCNGG 1 cut(s) 247
TaaI ACNGT 2 cut(s) 426, 438
TaiI ACGT 1 cut(s) 19
TaqI TCGA 1 cut(s) 231
TasI AATT 2 cut(s) 218, 370
TatI WGTACW 1 cut(s) 345
TseFI GTSAC 1 cut(s) 413
TseI GCWGC 2 cut(s) 191, 194
Tsp45I GTSAC 1 cut(s) 413
TspDTI ATGAA 3 cut(s) 101, 129, 290
TspGWI ACGGA 1 cut(s) 405
Van91I CCANNNNNTGG 1 cut(s) 127
XapI RAATTY 1 cut(s) 218
XmnI GAANNNNTTC 1 cut(s) 297
XspI CTAG 1 cut(s) 381
ZrmI AGTACT 1 cut(s) 347
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.