Rorug04G0018800

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0010331
Physical Location & Seq
Forward (+)
2802608 .. 2806692
4085 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0018800.1

Sequence Viewer

Length: 3648 bp
ATGGTTTCTCTGACCAATGAAGCAGCCGGTTCTTCGTCTTCTTCTCTCCCACGTTCTTCTATTGATCACCAAAATCATTACACATACGAGGTCTTCCTGAGTTTTCGAGGCGAGGATACACGCTTTAATTTTACAGATCATTTGTATACTGCTCTGTGTCAGAGGGGAATTGAGACCTTCAGAGATGATAAGCTTAGCAGAGGAGAAGACATATCACAAGAGCTCCTCAAAGCAATTGACGAGTCGAGAGTTTCAATCATAGTCTTCTCTCAAAATTATGCTGCCTCAAGGTGGTGCTTAGATGAACTGGTCAAGATACTTGAATGCAGAAAATCCAAAGGACAGGACGTTAGACCGGTGTTCTACAAGCTGGATCCTTCAGATGTACGACACCAAAGAGGTGCTTTCGGTGACTCATTTGCTACACTTGATCAATGCAAATACAAGGATAGCATGGACAAATGGAAGGCAGCTCTCAAGGAAGCAGCAGATTTGTCTGGATGGCCTTTCAAGGATGGCGAGTACGAGTCTAAATTTATCAATGACATTGTTGGGGAATTGTCTGCCCGAGTGGTAAACCCTTCATGTGAGTTGCAAGTAGCTGCACATCCTATTGGAATAGAGTCTTGTAGACAAGATGTCAACAAACTTTTGCACACCAGGGAAAATATTGTTCGCATGGTAGGTATATGGGGGCCTGGTGGAATAGGAAAGACCACAATTGCAAAAGATGTGTTTAATTCTATTCGCCAAAACTTCGAAGGTAGTTGTTTCTTGGCAGATGTTAGATCTAATTCAAATGGTCTAGCCCAACTACAAGAGACACTTTTGTCTAATATTTTAAACTCAACTTTGAAGGTGAGCAGTGTTGATGAAGGAGTGAGTTTAATAAAGACAAGGATGCGACATAAAAAAATTCTCTTAATCCTTGATGACGTGTCTCATTCCAGGCAATTACAGAACTTAGTTCCATCCCCTGATTGTTTTGGGCTGGGCAGCCGAATTCTCATAACAACAAGAGATAAACGTTTGCTAATTGCTCATCAAGTCGATGAAGTATACGAGGTCAAGATGTTAGATTATGATCAAGCTTTGGAGTTGTTTAGCTTAAATGCTTTCAAAAGAAATGGACCTCCAAGGGATTATCTGGAACTTGCACGACGTGCTATACGTTATGCCCAGGGCCTTCCATTAGCTTTGATTGTTTTAGGGTCTCATCTATTTTGTAGAAGCAAAGAGGAGTGGGAAGCTACATTAGGTAGTTGTAAAGAAGATCCCCACAGAGAGATAAGAGACGTGCTCAAAATAAGTTATGATGCTCTGGGAGTAGATTTAAAAGGATATTTTCTTGATATCGCTTGTTTCTTTAAGGGTAAGCCTGTAGACAATGTGAAACCAATACTAGAAGCTTGCTACGACCTCAAATCAGTGAATGGTATTGCACAACTCCAAGAAAAGGCCTTAATAAGAATTGATAGATTGCACTGGAGAAGTGGAATAGACAAAGGTTGTATTTGGATGCATGACTTGATAGAAGAAATGGGTAAAGACATAGTATATCAAGAGTCACCTGGTGAGCCCGGGGAACGCAGCAGAGTGTGGAGCAAAGAAGATGTCGACCACGTTCTAACAAATAATACAGGCACAGATAAAATTATAGGCATCCAGACCCATGTCATTTGGCCATATTCAAAGATATCTTTGAATGCTAAAAGCTTCTCAGGGATGAAGAATCTTAGATATATTTCAATGAGCAGAATAGAATATGAATGCTTTTCTGGAGACATTGATTATCTCTCCAACCAGTTAAGGTGGCTTGATTGGTCTAAATGTTCATTGCAATCTTTTCCATCTGATTTCCATGCAAACAAACTTGTAAATCTCGATATTTCTGAGAGTCGCGGAATCACACGGCTATGGGAGGGACGTAAGAATTTTTCAAGGCTAACATGTATGAATTTAAGAAGTTGTACATCCCTAAAGGAACTCCCAGACTTGAGTGCAATCCCCAACTTGAAAGAGTTGGATCTATCAGAGTGTACATCCCTAAAGGAACTCCCAGACTTGAGTGCAATCCCCAACTTGAAAGAGTTGAATCTATCAGAGTGTACATCCCTAAAGGAACTCCCAGACTTGAGTGCAATCCCCAATTTGAAAGAGTTGAATCTATCAGGGTGTCGCAGTTTAGTTGGGGTTCCTAATTCCGTAGGATTCCTTCGAAACCTTTTTACCTTGAATGTTGACGGCTGCTATAACCTTATTATGTTTCCAAGAAAAATCAACTGGAAATATGTAGAAACTATTTCTATCTGCTTTTGCAAGCTTGAGGAATTTTCAGAAGTTGGGAAAGAGATGGGTTCCTTGAGAAGTTTGGATCTATCACGCACTTGCATTAAAGAATTTGATGAGTCAATTGGAAATCTCATTGGGCTCGAAAAGTTGAAACTACTGGATTGTCAAAATCTTACAACTCTACCGCGTAGCATTTATGAGTTGCGTAATCTACAAGTTCTTGATGCGAGTAATTGCCCAAAACTTGCTGCATTTCCCGAAATTCTATTAAAGATGGATTCTTTGAGAGAACTTTTTCTATCAGGCAGTGACATTAGAGAATTGGGTGAGTCAATTGGAAATCTCATTGGGCTTGAAAAGTTGGAAATGCTGAATTGTCAAAATCTTACAACTCTGCCTTATAATATTTATGAGTTGTGCAATCTTCAAGTTCTTGATGCGAGTAATTGCCCAAAACTGGCTACATTTCCACAAATTCCAATAAAGATAGATTTCTTGAGAGAACTTTATTTAGGAGGCAGTGGCATTAGAGAATTGGATGAGTCAATCGTAAATCTCATTGGGCTTGAATGGTTGGATCTTGGTAATTGCAAAAATCTCACTACTCTACCGTGCAGCATTTACGGGTTGCAAAATTTAAAGAGGTTTGATCTTACTAACTGCTCGAAACTAGTCAGATTTCCAACAAATACCAAAATTTTGAATGTCGATGGTTGCTCACTATCACTTGCCAAGCTACAGGAGTTCAGTATCAGTGGATGCATGAGTTTATCAGATTGTGATTTCCTCATGACTCTTGATTGCTGGGAAACATTATACTTCCTTGATCTGTCATTTAACAATTTTGTTAGTCTTCCTGCTTGCATCACCAAGTTTGTCAACTTGCACGGGCTTGACTTGCGGGATTGCAAGAGACTCCGAGAAATTCCGGAGCTTCCACCATACGTTAGGCCAGACATAAGTGGTTGTGAATCACTGGAGAGATTCTCGAAATTGACAAATCCTAGAGCTGTGATAGAGGCGGCGTCACCGTCAATTGAAGAGCCAACTCCGTCTTCCGAAACTCCGAAACGACAACGCACTGCCACCGGGCAAGCATCGACGGTCCACTTTGACTATGAATTGACTATCTCCCCAATCCCTATGTTCCCTGACCTACACAGCTCCCATAACTCAACTACTCCTCCACAGCTTCAACACCAAGAGGAACCAACTGAGTTGTCATGGCTTTCTCTGTCTTCAGAACCAACAAAACCTGGTGGAGCAGTTAAAGGTGATCAAGACCGGTGGCTGTCTCTGTCTCTGCGACCAACACTTCAGAACTCTGAAGCATCCACTTCAGAGTTTAGACAATAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

1215

Amino Acids

137.19

Weight (kDa)

5.62

Isoelectric Point (pI)

44.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
TIR PF01582 29 - 190 1.1e-53 TIR domain
TIR_2 PF13676 31 - 126 1.9e-15 TIR domain
NB-ARC PF00931 215 - 375 9.2e-23 NB-ARC domain
WHD_ROQ1 PF23282 446 - 523 5.7e-10 Disease resistance protein Roq1-like, winged-helix domain
LRR_4 PF12799 693 - 726 9.3e-06 Leucine Rich repeats (2 copies)
LRR_13 PF23286 769 - 834 4.5e-06 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_14 PF23598 785 - 862 4.5e-09 Leucine-rich repeat region
LRR_13 PF23286 833 - 905 3.8e-08 Disease resistance protein RPS4B-like, leucine-rich repeats
LRR_13 PF23286 903 - 1098 5.7e-20 Disease resistance protein RPS4B-like, leucine-rich repeats
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 2694
AasI GACNNNNNNGTC 1 cut(s) 829
AccI GTMKAC 5 cut(s) 146, 631, 1059, 1383, 1617
AccII CGCG 2 cut(s) 1902, 2479
AccIII TCCGGA 1 cut(s) 3220
AciI CCGC 4 cut(s) 1902, 2477, 3193, 3314
AclI AACGTT 1 cut(s) 1027
AclWI GGATC 6 cut(s) 368, 381, 1268, 2034, 2382, 2877
AcoI YGGCCR 1 cut(s) 1682
AcuI CTGAAG 6 cut(s) 163, 363, 3516, 3593, 3615, 3639
AcyI GRCGYC 1 cut(s) 3317
AdeI CACNNNGTG 2 cut(s) 1163, 1574
AfaI GTAC 5 cut(s) 387, 524, 1972, 2041, 2110
AfiI CCNNNNNNNGG 3 cut(s) 291, 1456, 2749
AflIII ACRYGT 2 cut(s) 936, 1949
AgeI ACCGGT 2 cut(s) 355, 3576
AhdI GACNNNNNGTC 1 cut(s) 638
AhlI ACTAGT 1 cut(s) 2962
AjiI CACGTC 3 cut(s) 937, 1163, 1297
AjnI CCWGG 6 cut(s) 659, 697, 947, 1179, 1570, 3547
AjuI GAANNNNNNNTTGG 2 cut(s) 3331, 3363
Alw21I GWGCWC 2 cut(s) 225, 1302
Alw26I GTCTC 9 cut(s) 167, 815, 945, 1218, 1287, 1776, 3199, 3591, 3597
AlwI GGATC 6 cut(s) 368, 381, 1268, 2034, 2382, 2877
Ama87I CYCGRG 2 cut(s) 567, 1580
Aor13HI TCCGGA 1 cut(s) 3220
AoxI GGCC 6 cut(s) 503, 695, 1183, 1458, 1682, 3242
ArsI GACNNNNNNTTYG 2 cut(s) 1599, 1631
AsiGI ACCGGT 2 cut(s) 355, 3576
Asp700I GAANNNNTTC 1 cut(s) 2586
AspS9I GGNCC 4 cut(s) 695, 1130, 1183, 3397
AsuC2I CCSGG 3 cut(s) 1581, 1582, 3382
AsuHPI GGTGA 9 cut(s) 59, 422, 871, 1560, 1586, 2630, 3151, 3312, 3578
AsuII TTCGAA 2 cut(s) 759, 2218
AvaI CYCGRG 2 cut(s) 567, 1580
AvaII GGWCC 2 cut(s) 1130, 3397
BalI TGGCCA 1 cut(s) 1684
BamHI GGATCC 1 cut(s) 373
BanII GRGCYC 3 cut(s) 225, 1581, 2433
BarI GAAGNNNNNNTAC 2 cut(s) 2199, 2231
BbsI GAAGAC 7 cut(s) 30, 85, 213, 256, 3137, 3339, 3522
Bbv12I GWGCWC 2 cut(s) 225, 1302
BccI CCATC 7 cut(s) 495, 509, 979, 1858, 2347, 2560, 2996
BceAI ACGGC 2 cut(s) 1928, 2260
BciT130I CCWGG 6 cut(s) 661, 699, 949, 1181, 1572, 3549
BciVI GTATCC 1 cut(s) 109
BclI TGATCA 4 cut(s) 64, 430, 1084, 3568
BcnI CCSGG 3 cut(s) 1581, 1582, 3382
BcoDI GTCTC 9 cut(s) 167, 815, 945, 1218, 1287, 1776, 3199, 3591, 3597
BcuI ACTAGT 1 cut(s) 2962
BfaI CTAG 4 cut(s) 806, 1403, 2963, 3297
BfmI CTRYAG 2 cut(s) 1380, 3029
BfuI GTATCC 1 cut(s) 109
BglII AGATCT 1 cut(s) 788
BlpI GCTNAGC 1 cut(s) 194
Bme1390I CCNGG 9 cut(s) 661, 699, 949, 1181, 1572, 1581, 1582, 3382, 3549
Bme18I GGWCC 2 cut(s) 1130, 3397
BmeRI GACNNNNNGTC 1 cut(s) 638
BmeT110I CYCGRG 2 cut(s) 567, 1580
BmgBI CACGTC 3 cut(s) 937, 1163, 1297
BmgT120I GGNCC 4 cut(s) 695, 1130, 1183, 3397
BmiI GGNNCC 5 cut(s) 375, 696, 2196, 2359, 3501
BmrFI CCNGG 9 cut(s) 661, 699, 949, 1181, 1572, 1581, 1582, 3382, 3549
BoxI GACNNNNGTC 1 cut(s) 1673
BpiI GAAGAC 7 cut(s) 30, 85, 213, 256, 3137, 3339, 3522
BplI GAGNNNNNCTC 4 cut(s) 1284, 1316, 3263, 3295
BpmI CTGGAG 3 cut(s) 1507, 1800, 3290
Bpu1102I GCTNAGC 1 cut(s) 194
Bpu14I TTCGAA 2 cut(s) 759, 2218
BpuEI CTTGAG 8 cut(s) 271, 461, 2017, 2086, 2155, 2345, 2383, 2809
BpuMI CCSGG 3 cut(s) 1581, 1582, 3382
BsaBI GATNNNNATC 1 cut(s) 1083
BsaHI GRCGYC 1 cut(s) 3317
BsaI GGTCTC 2 cut(s) 167, 1218
BsaJI CCNNGG 6 cut(s) 660, 1136, 1179, 1180, 1580, 1581
BsaWI WCCGGW 3 cut(s) 355, 3220, 3576
BsaXI ACNNNNNCTCC 4 cut(s) 207, 237, 3460, 3490
Bsc4I CCNNNNNNNGG 3 cut(s) 291, 1456, 2749
Bse118I RCCGGY 3 cut(s) 26, 355, 3576
Bse1I ACTGG 7 cut(s) 312, 1490, 1804, 2288, 2454, 2754, 3273
Bse3DI GCAATG 1 cut(s) 1835
Bse8I GATNNNNATC 1 cut(s) 1083
BseAI TCCGGA 1 cut(s) 3220
BseBI CCWGG 6 cut(s) 661, 699, 949, 1181, 1572, 3549
BseDI CCNNGG 6 cut(s) 660, 1136, 1179, 1180, 1580, 1581
BseJI GATNNNNATC 1 cut(s) 1083
BseLI CCNNNNNNNGG 3 cut(s) 291, 1456, 2749
BseMI GCAATG 1 cut(s) 1835
BseMII CTCAG 4 cut(s) 89, 1734, 1884, 3498
BseNI ACTGG 7 cut(s) 312, 1490, 1804, 2288, 2454, 2754, 3273
BseRI GAGGAG 4 cut(s) 215, 216, 1253, 3465
BseYI CCCAGC 2 cut(s) 991, 3096
BsgI GTGCAG 2 cut(s) 588, 2926
Bsh1236I CGCG 2 cut(s) 1902, 2479
BshFI GGCC 6 cut(s) 505, 697, 1185, 1460, 1684, 3244
BshTI ACCGGT 2 cut(s) 355, 3576
BsiHKAI GWGCWC 2 cut(s) 225, 1302
BsiHKCI CYCGRG 2 cut(s) 567, 1580
BsiSI CCGG 6 cut(s) 27, 356, 1581, 3221, 3381, 3577
BslFI GGGAC 1 cut(s) 1938
BslI CCNNNNNNNGG 3 cut(s) 291, 1456, 2749
BsmAI GTCTC 9 cut(s) 167, 815, 945, 1218, 1287, 1776, 3199, 3591, 3597
BsmBI CGTCTC 1 cut(s) 1287
BsmFI GGGAC 1 cut(s) 1938
BsmI GAATGC 3 cut(s) 329, 1711, 1775
BsnI GGCC 6 cut(s) 505, 697, 1185, 1460, 1684, 3244
Bso31I GGTCTC 2 cut(s) 167, 1218
BsoBI CYCGRG 2 cut(s) 567, 1580
Bsp119I TTCGAA 2 cut(s) 759, 2218
Bsp1286I GDGCHC 4 cut(s) 225, 1302, 1581, 2433
Bsp13I TCCGGA 1 cut(s) 3220
Bsp1407I TGTACA 3 cut(s) 1970, 2039, 2108
Bsp1720I GCTNAGC 1 cut(s) 194
BspACI CCGC 4 cut(s) 1902, 2477, 3193, 3314
BspANI GGCC 6 cut(s) 505, 697, 1185, 1460, 1684, 3244
BspCNI CTCAG 4 cut(s) 90, 1733, 1885, 3499
BspEI TCCGGA 1 cut(s) 3220
BspFNI CGCG 2 cut(s) 1902, 2479
BspHI TCATGA 1 cut(s) 3081
BspLI GGNNCC 5 cut(s) 375, 696, 2196, 2359, 3501
BspPI GGATC 6 cut(s) 368, 381, 1268, 2034, 2382, 2877
BspQI GCTCTTC 1 cut(s) 3327
BspT104I TTCGAA 2 cut(s) 759, 2218
BspTNI GGTCTC 2 cut(s) 167, 1218
BsrDI GCAATG 1 cut(s) 1835
BsrFI RCCGGY 3 cut(s) 26, 355, 3576
BsrGI TGTACA 3 cut(s) 1970, 2039, 2108
BsrI ACTGG 7 cut(s) 312, 1490, 1804, 2288, 2454, 2754, 3273
BssAI RCCGGY 3 cut(s) 26, 355, 3576
BssECI CCNNGG 6 cut(s) 660, 1136, 1179, 1180, 1580, 1581
BssNAI GTATAC 2 cut(s) 147, 1060
BssNI GRCGYC 1 cut(s) 3317
BssT1I CCWWGG 1 cut(s) 1136
Bst1107I GTATAC 2 cut(s) 147, 1060
Bst2UI CCWGG 6 cut(s) 661, 699, 949, 1181, 1572, 3549
Bst4CI ACNGT 3 cut(s) 2904, 3324, 3397
Bst6I CTCTTC 1 cut(s) 3327
BstACI GRCGYC 1 cut(s) 3317
BstAPI GCANNNNNTGC 1 cut(s) 1163
BstAUI TGTACA 3 cut(s) 1970, 2039, 2108
BstBI TTCGAA 2 cut(s) 759, 2218
BstC8I GCNNGC 4 cut(s) 1411, 2321, 3154, 3387
BstDEI CTNAG 8 cut(s) 98, 194, 298, 964, 1720, 1736, 1893, 3507
BstFNI CGCG 2 cut(s) 1902, 2479
BstMAI GTCTC 9 cut(s) 167, 815, 945, 1218, 1287, 1776, 3199, 3591, 3597
BstMWI GCNNNNNNNGC 2 cut(s) 1163, 3190
BstNI CCWGG 6 cut(s) 661, 699, 949, 1181, 1572, 3549
BstNSI RCATGY 1 cut(s) 1953
BstPAI GACNNNNGTC 1 cut(s) 1673
BstSCI CCNGG 9 cut(s) 659, 697, 947, 1179, 1570, 1579, 1580, 3380, 3547
BstSFI CTRYAG 2 cut(s) 1380, 3029
BstUI CGCG 2 cut(s) 1902, 2479
BstV2I GAAGAC 7 cut(s) 30, 85, 213, 256, 3137, 3339, 3522
BstX2I RGATCY 6 cut(s) 373, 788, 1273, 2026, 2374, 2869
BstYI RGATCY 6 cut(s) 373, 788, 1273, 2026, 2374, 2869
BstZ17I GTATAC 2 cut(s) 147, 1060
BsuI GTATCC 1 cut(s) 109
BsuRI GGCC 6 cut(s) 505, 697, 1185, 1460, 1684, 3244
BtrI CACGTC 3 cut(s) 937, 1163, 1297
BtsI GCAGTG 4 cut(s) 871, 2605, 2818, 3372
BtsIMutI CAGTG 8 cut(s) 871, 1434, 1483, 2605, 2818, 3052, 3266, 3372
Cac8I GCNNGC 4 cut(s) 1411, 2321, 3154, 3387
CciI TCATGA 1 cut(s) 3081
Cfr10I RCCGGY 3 cut(s) 26, 355, 3576
Cfr13I GGNCC 4 cut(s) 695, 1130, 1183, 3397
Cfr9I CCCGGG 1 cut(s) 1580
CseI GACGC 1 cut(s) 3306
CsiI ACCWGGT 2 cut(s) 1570, 3547
Csp6I GTAC 5 cut(s) 386, 523, 1971, 2040, 2109
CspAI ACCGGT 2 cut(s) 355, 3576
CspCI CAANNNNNGTGG 2 cut(s) 3560, 3595
CviQI GTAC 5 cut(s) 386, 523, 1971, 2040, 2109
DdeI CTNAG 8 cut(s) 98, 194, 298, 964, 1720, 1736, 1893, 3507
DraI TTTAAA 3 cut(s) 843, 1335, 2931
DraIII CACNNNGTG 2 cut(s) 1163, 1574
DrdI GACNNNNNNGTC 1 cut(s) 829
DriI GACNNNNNGTC 1 cut(s) 638
DseDI GACNNNNNNGTC 1 cut(s) 829
EaeI YGGCCR 1 cut(s) 1682
Eam1104I CTCTTC 1 cut(s) 3327
Eam1105I GACNNNNNGTC 1 cut(s) 638
EarI CTCTTC 1 cut(s) 3327
Ecl136II GAGCTC 1 cut(s) 223
Eco130I CCWWGG 1 cut(s) 1136
Eco147I AGGCCT 1 cut(s) 1460
Eco24I GRGCYC 3 cut(s) 225, 1581, 2433
Eco31I GGTCTC 2 cut(s) 167, 1218
Eco32I GATATC 2 cut(s) 1354, 1698
Eco47I GGWCC 2 cut(s) 1130, 3397
Eco53kI GAGCTC 1 cut(s) 223
Eco57I CTGAAG 6 cut(s) 163, 363, 3516, 3593, 3615, 3639
Eco88I CYCGRG 2 cut(s) 567, 1580
EcoICRI GAGCTC 1 cut(s) 223
EcoO109I RGGNCCY 2 cut(s) 695, 1183
EcoRI GAATTC 1 cut(s) 1002
EcoRII CCWGG 6 cut(s) 659, 697, 947, 1179, 1570, 3547
EcoRV GATATC 2 cut(s) 1354, 1698
EcoT14I CCWWGG 1 cut(s) 1136
EcoT22I ATGCAT 2 cut(s) 1524, 3056
EcoT38I GRGCYC 3 cut(s) 225, 1581, 2433
ErhI CCWWGG 1 cut(s) 1136
Esp3I CGTCTC 1 cut(s) 1287
FalI AAGNNNNNCTT 4 cut(s) 388, 420, 810, 842
FaqI GGGAC 1 cut(s) 1938
FauI CCCGC 1 cut(s) 3186
FbaI TGATCA 4 cut(s) 64, 430, 1084, 3568
FblI GTMKAC 5 cut(s) 146, 631, 1059, 1383, 1617
FriOI GRGCYC 3 cut(s) 225, 1581, 2433
FspBI CTAG 4 cut(s) 806, 1403, 2963, 3297
GsaI CCCAGC 2 cut(s) 995, 3100
GsuI CTGGAG 3 cut(s) 1507, 1800, 3290
HaeIII GGCC 6 cut(s) 505, 697, 1185, 1460, 1684, 3244
HapII CCGG 6 cut(s) 27, 356, 1581, 3221, 3381, 3577
HgaI GACGC 1 cut(s) 3306
Hin1I GRCGYC 1 cut(s) 3317
HincII GTYRAC 4 cut(s) 643, 1618, 2242, 3172
HindII GTYRAC 4 cut(s) 643, 1618, 2242, 3172
HindIII AAGCTT 5 cut(s) 191, 1089, 1407, 1714, 2321
HpaII CCGG 6 cut(s) 27, 356, 1581, 3221, 3381, 3577
HphI GGTGA 9 cut(s) 59, 422, 871, 1560, 1586, 2630, 3151, 3312, 3578
Hpy99I CGWCG 2 cut(s) 1164, 3397
HpyAV CCTTC 9 cut(s) 187, 387, 460, 591, 755, 850, 869, 1196, 2225
HpyCH4III ACNGT 3 cut(s) 2904, 3324, 3397
HpyF10VI GCNNNNNNNGC 2 cut(s) 1163, 3190
HpyF3I CTNAG 8 cut(s) 98, 194, 298, 964, 1720, 1736, 1893, 3507
Hsp92I GRCGYC 1 cut(s) 3317
Kpn2I TCCGGA 1 cut(s) 3220
Ksp22I TGATCA 4 cut(s) 64, 430, 1084, 3568
LguI GCTCTTC 1 cut(s) 3327
LmnI GCTCC 5 cut(s) 228, 1602, 3223, 3461, 3554
MabI ACCWGGT 2 cut(s) 1570, 3547
MaeI CTAG 4 cut(s) 806, 1403, 2963, 3297
MaeIII GTNAC 4 cut(s) 410, 1566, 2600, 3318
MfeI CAATTG 5 cut(s) 234, 720, 2412, 2625, 3327
MflI RGATCY 6 cut(s) 373, 788, 1273, 2026, 2374, 2869
MhlI GDGCHC 4 cut(s) 225, 1302, 1581, 2433
MlsI TGGCCA 1 cut(s) 1684
MluNI TGGCCA 1 cut(s) 1684
MmeI TCCRAC 5 cut(s) 1824, 2004, 2634, 2847, 2999
Mox20I TGGCCA 1 cut(s) 1684
Mph1103I ATGCAT 2 cut(s) 1524, 3056
MroI TCCGGA 1 cut(s) 3220
MroXI GAANNNNTTC 1 cut(s) 2586
MscI TGGCCA 1 cut(s) 1684
MslI CAYNNNNRTG 1 cut(s) 1915
Msp20I TGGCCA 1 cut(s) 1684
MspI CCGG 6 cut(s) 27, 356, 1581, 3221, 3381, 3577
MspR9I CCNGG 9 cut(s) 661, 699, 949, 1181, 1572, 1581, 1582, 3382, 3549
MunI CAATTG 5 cut(s) 234, 720, 2412, 2625, 3327
Mva1269I GAATGC 3 cut(s) 329, 1711, 1775
MvaI CCWGG 6 cut(s) 661, 699, 949, 1181, 1572, 3549
MvnI CGCG 2 cut(s) 1902, 2479
MwoI GCNNNNNNNGC 2 cut(s) 1163, 3190
NciI CCSGG 3 cut(s) 1581, 1582, 3382
NlaIV GGNNCC 5 cut(s) 375, 696, 2196, 2359, 3501
NmuCI GTSAC 4 cut(s) 410, 1566, 2600, 3318
NsiI ATGCAT 2 cut(s) 1524, 3056
NspI RCATGY 1 cut(s) 1953
NspV TTCGAA 2 cut(s) 759, 2218
PagI TCATGA 1 cut(s) 3081
PasI CCCWGGG 1 cut(s) 1180
PceI AGGCCT 1 cut(s) 1460
PciI ACATGT 1 cut(s) 1949
PciSI GCTCTTC 1 cut(s) 3327
PcsI WCGNNNNNNNCGW 1 cut(s) 1168
PctI GAATGC 3 cut(s) 329, 1711, 1775
PdmI GAANNNNTTC 1 cut(s) 2586
PfeI GAWTC 8 cut(s) 1732, 1905, 2095, 2164, 2211, 2570, 3263, 3276
PinAI ACCGGT 2 cut(s) 355, 3576
PscI ACATGT 1 cut(s) 1949
PshAI GACNNNNGTC 1 cut(s) 1673
PsiI TTATAA 1 cut(s) 2694
Psp124BI GAGCTC 1 cut(s) 225
Psp1406I AACGTT 1 cut(s) 1027
Psp6I CCWGG 6 cut(s) 659, 697, 947, 1179, 1570, 3547
PspFI CCCAGC 2 cut(s) 991, 3096
PspGI CCWGG 6 cut(s) 659, 697, 947, 1179, 1570, 3547
PspN4I GGNNCC 5 cut(s) 375, 696, 2196, 2359, 3501
PspPI GGNCC 4 cut(s) 695, 1130, 1183, 3397
PsuI RGATCY 6 cut(s) 373, 788, 1273, 2026, 2374, 2869
RsaI GTAC 5 cut(s) 387, 524, 1972, 2041, 2110
RsaNI GTAC 5 cut(s) 386, 523, 1971, 2040, 2109
RseI CAYNNNNRTG 1 cut(s) 1915
SacI GAGCTC 1 cut(s) 225
SalI GTCGAC 1 cut(s) 1616
SapI GCTCTTC 1 cut(s) 3327
Sau96I GGNCC 4 cut(s) 695, 1130, 1183, 3397
ScrFI CCNGG 9 cut(s) 661, 699, 949, 1181, 1572, 1581, 1582, 3382, 3549
SduI GDGCHC 4 cut(s) 225, 1302, 1581, 2433
SexAI ACCWGGT 2 cut(s) 1570, 3547
SfcI CTRYAG 2 cut(s) 1380, 3029
SfuI TTCGAA 2 cut(s) 759, 2218
SinI GGWCC 2 cut(s) 1130, 3397
SmaI CCCGGG 1 cut(s) 1582
SmiMI CAYNNNNRTG 1 cut(s) 1915
SmlI CTYRAG 8 cut(s) 286, 476, 1996, 2065, 2134, 2324, 2362, 2788
SmoI CTYRAG 8 cut(s) 286, 476, 1996, 2065, 2134, 2324, 2362, 2788
SpeI ACTAGT 1 cut(s) 2962
SseBI AGGCCT 1 cut(s) 1460
SsiI CCGC 4 cut(s) 1902, 2477, 3193, 3314
SspI AATATT 3 cut(s) 670, 838, 2698
SspMI CTAG 4 cut(s) 806, 1403, 2963, 3297
SstI GAGCTC 1 cut(s) 225
StuI AGGCCT 1 cut(s) 1460
StyD4I CCNGG 9 cut(s) 659, 697, 947, 1179, 1570, 1579, 1580, 3380, 3547
StyI CCWWGG 1 cut(s) 1136
TaaI ACNGT 3 cut(s) 2904, 3324, 3397
TatI WGTACW 3 cut(s) 1970, 2039, 2108
TauI GCSGC 1 cut(s) 3317
TfiI GAWTC 8 cut(s) 1732, 1905, 2095, 2164, 2211, 2570, 3263, 3276
TscAI CASTG 8 cut(s) 871, 1434, 1490, 2605, 2818, 3052, 3273, 3379
TseFI GTSAC 4 cut(s) 410, 1566, 2600, 3318
Tsp45I GTSAC 4 cut(s) 410, 1566, 2600, 3318
TspGWI ACGGA 2 cut(s) 2194, 3333
TspMI CCCGGG 1 cut(s) 1580
TspRI CASTG 8 cut(s) 871, 1434, 1490, 2605, 2818, 3052, 3273, 3379
VpaK11BI GGWCC 2 cut(s) 1130, 3397
XceI RCATGY 1 cut(s) 1953
XmaI CCCGGG 1 cut(s) 1580
XmiI GTMKAC 5 cut(s) 146, 631, 1059, 1383, 1617
XmnI GAANNNNTTC 1 cut(s) 2586
XspI CTAG 4 cut(s) 806, 1403, 2963, 3297
Zsp2I ATGCAT 2 cut(s) 1524, 3056
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.