Rorug04G0019000

No description available

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000004
OG0010331
Physical Location & Seq
Reverse (-)
2822198 .. 2823547
1350 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug04G0019000.1

Sequence Viewer

Length: 1350 bp
ATGTCATCTTCAGCAGAAACCGTCACGAATGTCGAAGAGCTGCTTAATCAGATCCTTATATGGGTTCCAGCTCTATCTCTGATCCGTTTCAAGTGCGTCTCCAAGCAATGGCTGTCTCTCATCTCCGACCCCGAGTTCCGTCGCCGCCACACCCTCCGAAACCCTAACTCCAAAATCTCAGCTTTCTTCTCTAGCAAAATCCAAGACGAGTCCTTCAAGTCCATCCTTCTTGGTAACCATGAAATCCCACCTGGGTGGAATCCCTTCAAAACCCTTAACAATTCCGATTCCGTCCCTGATGAATCCAAGTTGAGGATTCTTCAGTCCTGCAATGGCCTCTTTCTCTGCCATATTCCTAGATCTGGACTTGAAACAAAAAATCATCCCGTATATGTTGTCAATCCCACAACCAACGAATTCCGGGCTCTTTCTTTTCCAGTTGTCAGACATAGTAGTGATTCTTTATTTGTGCGCTATGCTTTGGCTTTTGATCCTTCCATATCGCCTCATTACAAGGTGGTCTGCGTGAATAACTTCACCTTTTATTATAATAGGGGGAAGCACAAAATAGACATATATTCATCTAAGACCGGAGAGTGGAAGCATCTCGATACTCCTTTCTTCCCCAGACCCTCCGACGAGGGCAGGCACCATCATTTCATGAACAAGGCCATGCACTTTGACTGCAGGAGCAGAGAAGGCGCCATATACTGCAACGGCGCAGTTCATTGGATAAGAGACATAGGGGAAGCAAGCTTGCCATTCTATTCTTTCCCAGATGGTAAATTCATAAGAAACGAAGCTGATGTGCTGCACTACTTCGACATAGGCCAAGAGCGTTTTCTGGTTGCTTCAGCTACCCCTCCTGTCCCCCTGGTTGCCAAGAACTTTCCACCGTTATTGACTCAGAGATATTTTGGGGAGTGTGGTGGCTGTTTGTACTTGATTGAGATTTATCAGCATTGCAACACTCAATATTTTGAGGTCATGGAGATGGAGACGGACTACTCTGGCTGGTTTGTCAAGTATCGTCACTATCGTGTTATTCATGTTGATCTTAATCCGATAGTTGCAGCTCTTCCCGGACAGGATTGGAATGCGTTTGTTGTCTTGTGCCTTTCTGGGGAGGAGGAAACCTCCCAAGAAATAGATGACAAGGTTGAGGATTCTTCAACAGATTTTTTGTTGCACATGCCTGGTAAGATCATATCTTATAATCTTAGGAATAAGACCTTCAAGACATCTATTGAGTTAGCCAATAAGGAGCTTTATCTAACTTTAGGTGATCATAATCATTGTTTGCCAGAGGATATTATTTTTCCTTACATGGAGACTTTAGCTTGTTTGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
No domains found.

Protein Analysis

449

Amino Acids

51.77

Weight (kDa)

6.2

Isoelectric Point (pI)

55.59

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
F-box PF00646 12 - 48 3.7e-07 F-box domain
FBA_1 PF07734 90 - 282 2.4e-11 F-box associated beta propeller domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 549, 1215
AccB1I GGYRCC 2 cut(s) 648, 701
AccB7I CCANNNNNTGG 1 cut(s) 108
AciI CCGC 1 cut(s) 145
AclWI GGATC 3 cut(s) 46, 76, 485
AcsI RAATTY 2 cut(s) 416, 785
AcuI CTGAAG 2 cut(s) 305, 837
AcyI GRCGYC 1 cut(s) 702
AfaI GTAC 1 cut(s) 941
AfiI CCNNNNNNNGG 6 cut(s) 61, 108, 312, 362, 597, 1123
AgsI TTSAA 6 cut(s) 91, 217, 268, 371, 1173, 1237
AjnI CCWGG 3 cut(s) 250, 873, 1195
AjuI GAANNNNNNNTTGG 2 cut(s) 825, 857
AleI CACNNNNGTG 2 cut(s) 253, 1038
AluBI AGCT 9 cut(s) 40, 71, 182, 756, 803, 857, 1076, 1267, 1340
AluI AGCT 9 cut(s) 40, 71, 182, 756, 803, 857, 1076, 1267, 1340
Alw26I GTCTC 5 cut(s) 103, 120, 732, 992, 1325
AlwI GGATC 3 cut(s) 46, 76, 485
Ama87I CYCGRG 1 cut(s) 131
AoxI GGCC 3 cut(s) 334, 669, 829
ApeKI GCWGC 3 cut(s) 40, 811, 1073
ApoI RAATTY 2 cut(s) 416, 785
Asp700I GAANNNNTTC 2 cut(s) 263, 533
AspLEI GCGC 3 cut(s) 474, 704, 722
AsuC2I CCSGG 2 cut(s) 422, 1083
AsuHPI GGTGA 2 cut(s) 529, 1295
AvaI CYCGRG 1 cut(s) 131
BanI GGYRCC 2 cut(s) 648, 701
BanII GRGCYC 1 cut(s) 427
BbvI GCAGC 3 cut(s) 27, 798, 1085
BccI CCATC 4 cut(s) 230, 660, 773, 988
BceAI ACGGC 1 cut(s) 733
BciT130I CCWGG 3 cut(s) 252, 875, 1197
BclI TGATCA 1 cut(s) 1285
BcnI CCSGG 2 cut(s) 422, 1083
BcoDI GTCTC 5 cut(s) 103, 120, 732, 992, 1325
BfaI CTAG 2 cut(s) 192, 357
BfmI CTRYAG 1 cut(s) 685
BfoI RGCGCY 1 cut(s) 705
BglII AGATCT 1 cut(s) 359
BisI GCNGC 4 cut(s) 41, 145, 812, 1074
BlsI GCNGC 4 cut(s) 42, 146, 813, 1075
Bme1390I CCNGG 5 cut(s) 252, 422, 875, 1083, 1197
BmeT110I CYCGRG 1 cut(s) 131
BmiI GGNNCC 3 cut(s) 66, 650, 703
BmrFI CCNGG 5 cut(s) 252, 422, 875, 1083, 1197
BmsI GCATC 1 cut(s) 613
BplI GAGNNNNNCTC 2 cut(s) 1121, 1153
BpuMI CCSGG 2 cut(s) 422, 1083
BsaBI GATNNNNATC 2 cut(s) 1059, 1290
BsaHI GRCGYC 1 cut(s) 702
BsaJI CCNNGG 2 cut(s) 251, 873
BsaWI WCCGGW 1 cut(s) 590
BsaXI ACNNNNNCTCC 2 cut(s) 152, 182
Bsc4I CCNNNNNNNGG 6 cut(s) 61, 108, 312, 362, 597, 1123
Bse1I ACTGG 1 cut(s) 437
Bse3DI GCAATG 3 cut(s) 113, 337, 961
Bse8I GATNNNNATC 2 cut(s) 1059, 1290
BseBI CCWGG 3 cut(s) 252, 875, 1197
BseDI CCNNGG 2 cut(s) 251, 873
BseGI GGATG 2 cut(s) 222, 382
BseJI GATNNNNATC 2 cut(s) 1059, 1290
BseLI CCNNNNNNNGG 6 cut(s) 61, 108, 312, 362, 597, 1123
BseMI GCAATG 3 cut(s) 113, 337, 961
BseMII CTCAG 2 cut(s) 192, 920
BseNI ACTGG 1 cut(s) 437
BseRI GAGGAG 1 cut(s) 1142
BseXI GCAGC 3 cut(s) 27, 798, 1085
BsgI GTGCAG 1 cut(s) 797
BshFI GGCC 3 cut(s) 336, 671, 831
BshNI GGYRCC 2 cut(s) 648, 701
BsiHKCI CYCGRG 1 cut(s) 131
BsiSI CCGG 3 cut(s) 421, 591, 1083
BslFI GGGAC 2 cut(s) 278, 854
BslI CCNNNNNNNGG 6 cut(s) 61, 108, 312, 362, 597, 1123
BsmAI GTCTC 5 cut(s) 103, 120, 732, 992, 1325
BsmBI CGTCTC 2 cut(s) 103, 992
BsmFI GGGAC 2 cut(s) 278, 854
BsmI GAATGC 1 cut(s) 1102
BsnI GGCC 3 cut(s) 336, 671, 831
BsoBI CYCGRG 1 cut(s) 131
Bsp1286I GDGCHC 1 cut(s) 427
Bsp143I GATC 7 cut(s) 51, 81, 359, 490, 1054, 1203, 1285
BspACI CCGC 1 cut(s) 145
BspANI GGCC 3 cut(s) 336, 671, 831
BspCNI CTCAG 2 cut(s) 191, 919
BspHI TCATGA 1 cut(s) 660
BspLI GGNNCC 3 cut(s) 66, 650, 703
BspMAI CTGCAG 1 cut(s) 689
BspPI GGATC 3 cut(s) 46, 76, 485
BspQI GCTCTTC 2 cut(s) 30, 1083
BspT107I GGYRCC 2 cut(s) 648, 701
BsrDI GCAATG 3 cut(s) 113, 337, 961
BsrI ACTGG 1 cut(s) 437
BssECI CCNNGG 2 cut(s) 251, 873
BssMI GATC 7 cut(s) 51, 81, 359, 490, 1054, 1203, 1285
BssNI GRCGYC 1 cut(s) 702
Bst2UI CCWGG 3 cut(s) 252, 875, 1197
Bst4CI ACNGT 2 cut(s) 22, 897
Bst6I CTCTTC 2 cut(s) 30, 1083
BstACI GRCGYC 1 cut(s) 702
BstC8I GCNNGC 3 cut(s) 647, 754, 758
BstDEI CTNAG 4 cut(s) 178, 585, 906, 1220
BstEII GGTNACC 1 cut(s) 233
BstF5I GGATG 2 cut(s) 222, 382
BstH2I RGCGCY 1 cut(s) 705
BstHHI GCGC 3 cut(s) 474, 704, 722
BstKTI GATC 7 cut(s) 54, 84, 362, 493, 1057, 1206, 1288
BstMAI GTCTC 5 cut(s) 103, 120, 732, 992, 1325
BstMBI GATC 7 cut(s) 51, 81, 359, 490, 1054, 1203, 1285
BstMWI GCNNNNNNNGC 1 cut(s) 699
BstNI CCWGG 3 cut(s) 252, 875, 1197
BstNSI RCATGY 1 cut(s) 1195
BstPI GGTNACC 1 cut(s) 233
BstSCI CCNGG 5 cut(s) 250, 420, 873, 1081, 1195
BstSFI CTRYAG 1 cut(s) 685
BstV1I GCAGC 3 cut(s) 27, 798, 1085
BstX2I RGATCY 2 cut(s) 51, 359
BstXI CCANNNNNNTGG 1 cut(s) 255
BstYI RGATCY 2 cut(s) 51, 359
BsuRI GGCC 3 cut(s) 336, 671, 831
BtsCI GGATG 2 cut(s) 222, 382
Cac8I GCNNGC 3 cut(s) 647, 754, 758
CciI TCATGA 1 cut(s) 660
CfoI GCGC 3 cut(s) 474, 704, 722
CseI GACGC 1 cut(s) 85
Csp6I GTAC 1 cut(s) 940
CspCI CAANNNNNGTGG 2 cut(s) 882, 917
CviAII CATG 7 cut(s) 239, 661, 673, 988, 1049, 1192, 1327
CviQI GTAC 1 cut(s) 940
DdeI CTNAG 4 cut(s) 178, 585, 906, 1220
DinI GGCGCC 1 cut(s) 703
DpnI GATC 7 cut(s) 53, 83, 361, 492, 1056, 1205, 1287
DpnII GATC 7 cut(s) 51, 81, 359, 490, 1054, 1203, 1285
Eam1104I CTCTTC 2 cut(s) 30, 1083
EarI CTCTTC 2 cut(s) 30, 1083
Eco24I GRGCYC 1 cut(s) 427
Eco57I CTGAAG 2 cut(s) 305, 837
Eco88I CYCGRG 1 cut(s) 131
Eco91I GGTNACC 1 cut(s) 233
EcoO65I GGTNACC 1 cut(s) 233
EcoRI GAATTC 1 cut(s) 416
EcoRII CCWGG 3 cut(s) 250, 873, 1195
EcoT38I GRGCYC 1 cut(s) 427
EgeI GGCGCC 1 cut(s) 703
EheI GGCGCC 1 cut(s) 703
Esp3I CGTCTC 2 cut(s) 103, 992
FaeI CATG 7 cut(s) 242, 664, 676, 991, 1052, 1195, 1330
FalI AAGNNNNNCTT 2 cut(s) 27, 59
FaqI GGGAC 2 cut(s) 278, 854
FatI CATG 7 cut(s) 238, 660, 672, 987, 1048, 1191, 1326
FbaI TGATCA 1 cut(s) 1285
Fnu4HI GCNGC 4 cut(s) 41, 145, 812, 1074
FokI GGATG 2 cut(s) 209, 369
FriOI GRGCYC 1 cut(s) 427
Fsp4HI GCNGC 4 cut(s) 41, 145, 812, 1074
FspBI CTAG 2 cut(s) 192, 357
GlaI GCGC 3 cut(s) 473, 703, 721
GluI GCNGC 4 cut(s) 41, 145, 812, 1074
HaeII RGCGCY 1 cut(s) 705
HaeIII GGCC 3 cut(s) 336, 671, 831
HapII CCGG 3 cut(s) 421, 591, 1083
HgaI GACGC 1 cut(s) 85
HhaI GCGC 3 cut(s) 474, 704, 722
Hin1I GRCGYC 1 cut(s) 702
Hin1II CATG 7 cut(s) 242, 664, 676, 991, 1052, 1195, 1330
Hin6I GCGC 3 cut(s) 472, 702, 720
HinP1I GCGC 3 cut(s) 472, 702, 720
HindIII AAGCTT 1 cut(s) 754
HinfI GANTC 8 cut(s) 209, 259, 287, 302, 316, 458, 904, 1166
HpaII CCGG 3 cut(s) 421, 591, 1083
HphI GGTGA 2 cut(s) 529, 1295
Hpy188I TCNGA 9 cut(s) 51, 81, 127, 158, 286, 446, 637, 909, 1065
Hpy188III TCNNGA 5 cut(s) 25, 363, 608, 661, 1237
Hpy99I CGWCG 2 cut(s) 144, 641
HpyAV CCTTC 6 cut(s) 223, 236, 274, 504, 692, 1243
HpyCH4III ACNGT 2 cut(s) 22, 897
HpyCH4V TGCA 8 cut(s) 330, 676, 687, 714, 814, 966, 1073, 1189
HpyF10VI GCNNNNNNNGC 1 cut(s) 699
HpyF3I CTNAG 4 cut(s) 178, 585, 906, 1220
Hsp92I GRCGYC 1 cut(s) 702
Hsp92II CATG 7 cut(s) 242, 664, 676, 991, 1052, 1195, 1330
HspAI GCGC 3 cut(s) 472, 702, 720
KasI GGCGCC 1 cut(s) 701
Ksp22I TGATCA 1 cut(s) 1285
Kzo9I GATC 7 cut(s) 51, 81, 359, 490, 1054, 1203, 1285
LguI GCTCTTC 2 cut(s) 30, 1083
LmnI GCTCC 2 cut(s) 690, 1264
Lsp1109I GCAGC 3 cut(s) 27, 798, 1085
LweI GCATC 1 cut(s) 613
MaeI CTAG 2 cut(s) 192, 357
MaeIII GTNAC 3 cut(s) 22, 233, 1031
MalI GATC 7 cut(s) 53, 83, 361, 492, 1056, 1205, 1287
MboI GATC 7 cut(s) 51, 81, 359, 490, 1054, 1203, 1285
MboII GAAGA 6 cut(s) 47, 178, 311, 613, 1070, 1161
MflI RGATCY 2 cut(s) 51, 359
MhlI GDGCHC 1 cut(s) 427
MluCI AATT 3 cut(s) 280, 416, 785
Mly113I GGCGCC 1 cut(s) 702
MlyI GAGTC 2 cut(s) 218, 898
MmeI TCCRAC 2 cut(s) 150, 660
MroXI GAANNNNTTC 2 cut(s) 263, 533
MseI TTAA 3 cut(s) 45, 276, 1059
MslI CAYNNNNRTG 4 cut(s) 253, 453, 992, 1038
MspI CCGG 3 cut(s) 421, 591, 1083
MspR9I CCNGG 5 cut(s) 252, 422, 875, 1083, 1197
Mva1269I GAATGC 1 cut(s) 1102
MvaI CCWGG 3 cut(s) 252, 875, 1197
MwoI GCNNNNNNNGC 1 cut(s) 699
NarI GGCGCC 1 cut(s) 702
NciI CCSGG 2 cut(s) 422, 1083
NdeII GATC 7 cut(s) 51, 81, 359, 490, 1054, 1203, 1285
NlaIII CATG 7 cut(s) 242, 664, 676, 991, 1052, 1195, 1330
NlaIV GGNNCC 3 cut(s) 66, 650, 703
NmuCI GTSAC 2 cut(s) 22, 1031
NspI RCATGY 1 cut(s) 1195
OliI CACNNNNGTG 2 cut(s) 253, 1038
PagI TCATGA 1 cut(s) 660
PciSI GCTCTTC 2 cut(s) 30, 1083
PcsI WCGNNNNNNNCGW 1 cut(s) 1036
PctI GAATGC 1 cut(s) 1102
PdmI GAANNNNTTC 2 cut(s) 263, 533
PfeI GAWTC 6 cut(s) 259, 287, 302, 316, 458, 1166
PflMI CCANNNNNTGG 1 cut(s) 108
PfoI TCCNGGA 1 cut(s) 1081
PkrI GCNGC 4 cut(s) 42, 146, 813, 1075
PleI GAGTC 2 cut(s) 217, 898
PluTI GGCGCC 1 cut(s) 705
PpsI GAGTC 2 cut(s) 217, 898
PsiI TTATAA 2 cut(s) 549, 1215
Psp6I CCWGG 3 cut(s) 250, 873, 1195
PspEI GGTNACC 1 cut(s) 233
PspGI CCWGG 3 cut(s) 250, 873, 1195
PspN4I GGNNCC 3 cut(s) 66, 650, 703
PstI CTGCAG 1 cut(s) 689
PsuI RGATCY 2 cut(s) 51, 359
RsaI GTAC 1 cut(s) 941
RsaNI GTAC 1 cut(s) 940
RseI CAYNNNNRTG 4 cut(s) 253, 453, 992, 1038
SapI GCTCTTC 2 cut(s) 30, 1083
SaqAI TTAA 3 cut(s) 45, 276, 1059
SatI GCNGC 4 cut(s) 41, 145, 812, 1074
Sau3AI GATC 7 cut(s) 51, 81, 359, 490, 1054, 1203, 1285
SchI GAGTC 2 cut(s) 218, 898
ScrFI CCNGG 5 cut(s) 252, 422, 875, 1083, 1197
SduI GDGCHC 1 cut(s) 427
SfaNI GCATC 1 cut(s) 613
SfcI CTRYAG 1 cut(s) 685
SfoI GGCGCC 1 cut(s) 703
SmiMI CAYNNNNRTG 4 cut(s) 253, 453, 992, 1038
Sse9I AATT 3 cut(s) 280, 416, 785
SsiI CCGC 1 cut(s) 145
SspDI GGCGCC 1 cut(s) 701
SspI AATATT 1 cut(s) 977
SspMI CTAG 2 cut(s) 192, 357
StyD4I CCNGG 5 cut(s) 250, 420, 873, 1081, 1195
TaaI ACNGT 2 cut(s) 22, 897
TaqI TCGA 3 cut(s) 33, 609, 822
TasI AATT 3 cut(s) 280, 416, 785
TatI WGTACW 1 cut(s) 939
TauI GCSGC 1 cut(s) 147
TfiI GAWTC 6 cut(s) 259, 287, 302, 316, 458, 1166
Tru1I TTAA 3 cut(s) 45, 276, 1059
Tru9I TTAA 3 cut(s) 45, 276, 1059
TseFI GTSAC 2 cut(s) 22, 1031
TseI GCWGC 3 cut(s) 40, 811, 1073
Tsp45I GTSAC 2 cut(s) 22, 1031
TspDTI ATGAA 8 cut(s) 255, 315, 570, 649, 677, 716, 778, 1037
TspGWI ACGGA 4 cut(s) 74, 128, 280, 1016
Van91I CCANNNNNTGG 1 cut(s) 108
XapI RAATTY 2 cut(s) 416, 785
XceI RCATGY 1 cut(s) 1195
XmnI GAANNNNTTC 2 cut(s) 263, 533
XspI CTAG 2 cut(s) 192, 357
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.