AT5G58320

Kinase interacting

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0005248
Physical Location & Seq
Forward (+)
23577128 .. 23579786
2659 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G58320.2

Sequence Viewer

Length: 1578 bp
ATGGATTATGATCTGCTTCGTTCCAAGAAGAGTATCAAACGCGTAGAATCCACCAAATCAAATCCATGGTGGTGGGATAGTCATATCGGTCTCAAAAATTCTAAGTGGCTTGAGAATAATCTCGATGAGATGGACCGGAGCGTGAAACGGATGGTGAAGTTGATAGAGGAGGATGCAGATTCTTTTGCTAAGAAGGCAGAGATGTATTACCAGTCAAGACCTGAGCTTATTGCTCTTGTGGATGAGTTTCACCGTATGTACCGTGCTTTGGCAGAGCGGTACGAGAACATCACCGGAGAGTTGAGAAAAGGCTCTCCTTTAGAGCTTCAGTCACAGGGCTCTGGTCTTTCTGACATCTCTGCTTCTGACCTCTCTGCTTTATGGACCTCTAATGAGGTTAATAGGTTAGGTCGTCCTCCTTCTGGTCGACGTGCTCCTGGTTTTGAATACTTCCTTGGCAATGGAGGACTTCCTTCTGATCTTTATCACAAAGATGGAGATGATTCTGCTTCCATTACTGATTCTGAACTTGAATCTGATGATTCTTCTGTGACTAATTACCCTGGTTATGTGAGTATAGGCTCTGACTTTCAGTCCCTAAGCAAGAGGATCATGGATCTTGAGATTGAGCTACGTGAAGCCAAAGAAAGACTTAGGATGCAACTTGAAGGGAACACTGAGAGCTTGTTGCCGAGGGTGAAAAGTGAGACTAAATTTGTTGATTTCCCGGCAAAACTTGCTGCTTGTGAGCAAGAACTGAAAGATGTAAATGAGAAGTTACAGAATTCAGAAGACCAAATCTATATTTTAAAGAGTCAGCTTGCTAGATACTTGCCATCTGGGTTGGATGATGAACAGAGCGAAGGGGCAGCTTCAACTCAAGAACTGGACATTGAGACTTTGTCTGAAGAGCTGAGAATTACAAGCCTGAGGCTTCGGGAGGCAGAAAAACAAAACGGTATCATGAGAAAAGAAGTCGAGAAAAGCAAGTCTGATGATGCAAAACTCAAGAGTCTGCAAGACATGCTTGAGTCGGCACAGAAAGAAGCTGCAGCATGGAAAAGCAAGGCTAGTGCAGATAAAAGAGAGGTGGTGAAGCTGCTTGATAGAATCTCAATGTTGAAGTCGAGTTTAGCAGGTAGAGATCATGAGATCAGGGATTTAAAGACAGCATTGTCTGACGCAGAAGAGAAGATTTTCCCGGAGAAAGCACAGGTCAAAGCCGATATAGCAAAGCTGTTGGAAGAAAAAATACACCGGGATGACCAATTCAAAGAGCTGGAAGCCAATGTGCGTTATCTTGAAGATGAGAGAAGAAAGGTGAACAATGAGAAAATAGAAGAGGAGGAAAAGCTGAAGAGCGAAATCGAGGTGCTGACACTGGAAAAGGTAGAGAAAGGGAGATGCATAGAGACGTTAAGCAGAAAGGTGAGTGAGCTAGAGTCGGAGATAAGTCGACTTGGAATGATAAGACAGCTCTGTTTTTCACTAGACTACTCCAGAGACGAATACAAGAGACTACGCATAGCCTTTTCAGGGCATCCACCAACAAGACCATCATCCATTCTTGCTTCTTAA

Protein Analysis

525

Amino Acids

60.12

Weight (kDa)

5.22

Isoelectric Point (pI)

53.65

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 23 - 95 1.6e-35 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1174
Acc36I ACCTGC 1 cut(s) 1127
AccBSI CCGCTC 1 cut(s) 277
AccI GTMKAC 2 cut(s) 427, 1456
AccII CGCG 1 cut(s) 42
AciI CCGC 1 cut(s) 277
AclWI GGATC 2 cut(s) 617, 624
AcsI RAATTY 3 cut(s) 97, 713, 784
AcuI CTGAAG 3 cut(s) 311, 927, 1376
AfaI GTAC 2 cut(s) 260, 281
AfiI CCNNNNNNNGG 3 cut(s) 268, 422, 1536
AflIII ACRYGT 1 cut(s) 40
AgsI TTSAA 7 cut(s) 446, 533, 668, 876, 1123, 1273, 1304
AhdI GACNNNNNGTC 1 cut(s) 592
AjiI CACGTC 1 cut(s) 431
AjnI CCWGG 2 cut(s) 436, 562
AjuI GAANNNNNNNTTGG 2 cut(s) 438, 470
Alw21I GWGCWC 1 cut(s) 436
Alw26I GTCTC 6 cut(s) 95, 701, 890, 1406, 1497, 1510
AlwI GGATC 2 cut(s) 617, 624
ApeKI GCWGC 5 cut(s) 740, 869, 1049, 1052, 1099
ApoI RAATTY 3 cut(s) 97, 713, 784
Asp700I GAANNNNTTC 1 cut(s) 1196
AspS9I GGNCC 2 cut(s) 133, 384
AsuC2I CCSGG 3 cut(s) 728, 1202, 1259
AsuHPI GGTGA 7 cut(s) 166, 242, 283, 709, 1105, 1333, 1441
AvaII GGWCC 2 cut(s) 133, 384
AxyI CCTNAGG 1 cut(s) 929
BanII GRGCYC 1 cut(s) 341
BarI GAAGNNNNNNTAC 2 cut(s) 1236, 1268
BbsI GAAGAC 1 cut(s) 798
Bbv12I GWGCWC 1 cut(s) 436
BbvI GCAGC 5 cut(s) 727, 881, 1036, 1064, 1086
BccI CCATC 5 cut(s) 124, 145, 488, 844, 1564
BciT130I CCWGG 2 cut(s) 438, 564
BcnI CCSGG 3 cut(s) 728, 1202, 1259
BcoDI GTCTC 6 cut(s) 95, 701, 890, 1406, 1497, 1510
BfaI CTAG 4 cut(s) 825, 1071, 1439, 1490
BfmI CTRYAG 1 cut(s) 1050
BfuAI ACCTGC 1 cut(s) 1127
BisI GCNGC 5 cut(s) 741, 870, 1050, 1053, 1100
BlsI GCNGC 5 cut(s) 742, 871, 1051, 1054, 1101
Bme1390I CCNGG 5 cut(s) 438, 564, 728, 1202, 1259
Bme18I GGWCC 2 cut(s) 133, 384
BmeRI GACNNNNNGTC 1 cut(s) 592
BmgBI CACGTC 1 cut(s) 431
BmgT120I GGNCC 2 cut(s) 133, 384
BmrFI CCNGG 5 cut(s) 438, 564, 728, 1202, 1259
BmsI GCATC 5 cut(s) 163, 648, 988, 1394, 1549
BpiI GAAGAC 1 cut(s) 798
BpmI CTGGAG 1 cut(s) 1483
Bpu10I CCTNAGC 2 cut(s) 222, 599
BpuEI CTTGAG 5 cut(s) 131, 641, 864, 992, 1049
BpuMI CCSGG 3 cut(s) 728, 1202, 1259
BsaAI YACGTR 1 cut(s) 635
BsaBI GATNNNNATC 2 cut(s) 9, 483
BsaI GGTCTC 1 cut(s) 95
BsaJI CCNNGG 4 cut(s) 65, 454, 562, 692
BsaWI WCCGGW 2 cut(s) 135, 293
Bsc4I CCNNNNNNNGG 3 cut(s) 268, 422, 1536
Bse1I ACTGG 3 cut(s) 211, 891, 1386
Bse21I CCTNAGG 1 cut(s) 929
Bse3DI GCAATG 1 cut(s) 466
Bse8I GATNNNNATC 2 cut(s) 9, 483
BseBI CCWGG 2 cut(s) 438, 564
BseDI CCNNGG 4 cut(s) 65, 454, 562, 692
BseGI GGATG 8 cut(s) 156, 178, 247, 663, 853, 1267, 1540, 1559
BseJI GATNNNNATC 2 cut(s) 9, 483
BseLI CCNNNNNNNGG 3 cut(s) 268, 422, 1536
BseMI GCAATG 1 cut(s) 466
BseMII CTCAG 4 cut(s) 213, 669, 905, 920
BseNI ACTGG 3 cut(s) 211, 891, 1386
BseRI GAGGAG 2 cut(s) 182, 1358
BseXI GCAGC 5 cut(s) 727, 881, 1036, 1064, 1086
BsgI GTGCAG 1 cut(s) 1095
Bsh1236I CGCG 1 cut(s) 42
BsiHKAI GWGCWC 1 cut(s) 436
BsiSI CCGG 5 cut(s) 136, 294, 728, 1202, 1258
BslFI GGGAC 1 cut(s) 580
BslI CCNNNNNNNGG 3 cut(s) 268, 422, 1536
BsmAI GTCTC 6 cut(s) 95, 701, 890, 1406, 1497, 1510
BsmBI CGTCTC 2 cut(s) 1406, 1497
BsmFI GGGAC 1 cut(s) 580
Bso31I GGTCTC 1 cut(s) 95
Bsp1286I GDGCHC 2 cut(s) 341, 436
Bsp143I GATC 6 cut(s) 10, 478, 609, 616, 1144, 1152
Bsp19I CCATGG 1 cut(s) 65
BspACI CCGC 1 cut(s) 277
BspCNI CTCAG 4 cut(s) 214, 670, 906, 921
BspFNI CGCG 1 cut(s) 42
BspHI TCATGA 2 cut(s) 963, 1147
BspMAI CTGCAG 1 cut(s) 1054
BspMI ACCTGC 1 cut(s) 1127
BspPI GGATC 2 cut(s) 617, 624
BspQI GCTCTTC 2 cut(s) 903, 1352
BspTNI GGTCTC 1 cut(s) 95
BsrBI CCGCTC 1 cut(s) 277
BsrDI GCAATG 1 cut(s) 466
BsrI ACTGG 3 cut(s) 211, 891, 1386
BssECI CCNNGG 4 cut(s) 65, 454, 562, 692
BssMI GATC 6 cut(s) 10, 478, 609, 616, 1144, 1152
BssT1I CCWWGG 2 cut(s) 65, 454
Bst2UI CCWGG 2 cut(s) 438, 564
Bst4CI ACNGT 3 cut(s) 254, 263, 959
Bst6I CTCTTC 5 cut(s) 23, 903, 1182, 1335, 1352
BstAPI GCANNNNNTGC 2 cut(s) 737, 1024
BstBAI YACGTR 1 cut(s) 635
BstC8I GCNNGC 1 cut(s) 822
BstDEI CTNAG 8 cut(s) 102, 189, 222, 599, 653, 678, 914, 929
BstDSI CCRYGG 1 cut(s) 65
BstF5I GGATG 8 cut(s) 156, 178, 247, 663, 853, 1267, 1540, 1559
BstFNI CGCG 1 cut(s) 42
BstKTI GATC 6 cut(s) 13, 481, 612, 619, 1147, 1155
BstMAI GTCTC 6 cut(s) 95, 701, 890, 1406, 1497, 1510
BstMBI GATC 6 cut(s) 10, 478, 609, 616, 1144, 1152
BstMWI GCNNNNNNNGC 4 cut(s) 194, 737, 1024, 1229
BstNI CCWGG 2 cut(s) 438, 564
BstNSI RCATGY 1 cut(s) 1027
BstSCI CCNGG 5 cut(s) 436, 562, 726, 1200, 1257
BstSFI CTRYAG 1 cut(s) 1050
BstUI CGCG 1 cut(s) 42
BstV1I GCAGC 5 cut(s) 727, 881, 1036, 1064, 1086
BstV2I GAAGAC 1 cut(s) 798
BstX2I RGATCY 1 cut(s) 616
BstXI CCANNNNNNTGG 1 cut(s) 72
BstYI RGATCY 1 cut(s) 616
Bsu36I CCTNAGG 1 cut(s) 929
BtgI CCRYGG 1 cut(s) 65
BtrI CACGTC 1 cut(s) 431
BtsCI GGATG 8 cut(s) 156, 178, 247, 663, 853, 1267, 1540, 1559
BtsIMutI CAGTG 2 cut(s) 675, 1379
BveI ACCTGC 1 cut(s) 1127
Cac8I GCNNGC 1 cut(s) 822
CciI TCATGA 2 cut(s) 963, 1147
Cfr13I GGNCC 2 cut(s) 133, 384
CseI GACGC 1 cut(s) 1190
Csp6I GTAC 2 cut(s) 259, 280
CviAII CATG 6 cut(s) 66, 613, 964, 1024, 1056, 1148
CviQI GTAC 2 cut(s) 259, 280
DdeI CTNAG 8 cut(s) 102, 189, 222, 599, 653, 678, 914, 929
DpnI GATC 6 cut(s) 12, 480, 611, 618, 1146, 1154
DpnII GATC 6 cut(s) 10, 478, 609, 616, 1144, 1152
DraI TTTAAA 2 cut(s) 810, 1164
DrdI GACNNNNNNGTC 1 cut(s) 1174
DriI GACNNNNNGTC 1 cut(s) 592
DseDI GACNNNNNNGTC 1 cut(s) 1174
Eam1104I CTCTTC 5 cut(s) 23, 903, 1182, 1335, 1352
Eam1105I GACNNNNNGTC 1 cut(s) 592
EarI CTCTTC 5 cut(s) 23, 903, 1182, 1335, 1352
Eco130I CCWWGG 2 cut(s) 65, 454
Eco24I GRGCYC 1 cut(s) 341
Eco31I GGTCTC 1 cut(s) 95
Eco47I GGWCC 2 cut(s) 133, 384
Eco57I CTGAAG 3 cut(s) 311, 927, 1376
Eco81I CCTNAGG 1 cut(s) 929
EcoRI GAATTC 1 cut(s) 784
EcoRII CCWGG 2 cut(s) 436, 562
EcoT14I CCWWGG 2 cut(s) 65, 454
EcoT22I ATGCAT 1 cut(s) 1409
EcoT38I GRGCYC 1 cut(s) 341
ErhI CCWWGG 2 cut(s) 65, 454
Esp3I CGTCTC 2 cut(s) 1406, 1497
FaeI CATG 6 cut(s) 69, 616, 967, 1027, 1059, 1151
FalI AAGNNNNNCTT 4 cut(s) 636, 668, 1011, 1043
FaqI GGGAC 1 cut(s) 580
FatI CATG 6 cut(s) 65, 612, 963, 1023, 1055, 1147
FblI GTMKAC 2 cut(s) 427, 1456
Fnu4HI GCNGC 5 cut(s) 741, 870, 1050, 1053, 1100
FokI GGATG 8 cut(s) 163, 185, 254, 670, 860, 1274, 1527, 1546
FriOI GRGCYC 1 cut(s) 341
Fsp4HI GCNGC 5 cut(s) 741, 870, 1050, 1053, 1100
FspBI CTAG 4 cut(s) 825, 1071, 1439, 1490
GluI GCNGC 5 cut(s) 741, 870, 1050, 1053, 1100
GsuI CTGGAG 1 cut(s) 1483
HapII CCGG 5 cut(s) 136, 294, 728, 1202, 1258
HgaI GACGC 1 cut(s) 1190
Hin1II CATG 6 cut(s) 69, 616, 967, 1027, 1059, 1151
HincII GTYRAC 2 cut(s) 428, 1457
HindII GTYRAC 2 cut(s) 428, 1457
HpaII CCGG 5 cut(s) 136, 294, 728, 1202, 1258
HphI GGTGA 7 cut(s) 166, 242, 283, 709, 1105, 1333, 1441
Hpy166II GTNNAC 3 cut(s) 428, 1324, 1457
Hpy8I GTNNAC 3 cut(s) 428, 1324, 1457
Hpy99I CGWCG 1 cut(s) 432
HpyAV CCTTC 5 cut(s) 187, 429, 483, 662, 857
HpyCH4III ACNGT 3 cut(s) 254, 263, 959
HpyCH4IV ACGT 3 cut(s) 430, 634, 1415
HpyCH4V TGCA 7 cut(s) 176, 661, 1001, 1018, 1052, 1076, 1407
HpyF10VI GCNNNNNNNGC 4 cut(s) 194, 737, 1024, 1229
HpyF3I CTNAG 8 cut(s) 102, 189, 222, 599, 653, 678, 914, 929
HpySE526I ACGT 3 cut(s) 430, 634, 1415
Hsp92II CATG 6 cut(s) 69, 616, 967, 1027, 1059, 1151
Kzo9I GATC 6 cut(s) 10, 478, 609, 616, 1144, 1152
LguI GCTCTTC 2 cut(s) 903, 1352
LmnI GCTCC 2 cut(s) 138, 439
Lsp1109I GCAGC 5 cut(s) 727, 881, 1036, 1064, 1086
LweI GCATC 5 cut(s) 163, 648, 988, 1394, 1549
MaeI CTAG 4 cut(s) 825, 1071, 1439, 1490
MaeII ACGT 3 cut(s) 430, 634, 1415
MaeIII GTNAC 3 cut(s) 330, 550, 777
MalI GATC 6 cut(s) 12, 480, 611, 618, 1146, 1154
MbiI CCGCTC 1 cut(s) 277
MboI GATC 6 cut(s) 10, 478, 609, 616, 1144, 1152
MflI RGATCY 1 cut(s) 616
MhlI GDGCHC 2 cut(s) 341, 436
MluCI AATT 6 cut(s) 97, 556, 713, 784, 918, 1268
MluI ACGCGT 1 cut(s) 40
MlyI GAGTC 4 cut(s) 823, 1021, 1040, 1451
MmeI TCCRAC 3 cut(s) 825, 1221, 1425
Mph1103I ATGCAT 1 cut(s) 1409
MroXI GAANNNNTTC 1 cut(s) 1196
MseI TTAA 5 cut(s) 399, 809, 1163, 1418, 1576
MslI CAYNNNNRTG 3 cut(s) 70, 492, 1260
MspI CCGG 5 cut(s) 136, 294, 728, 1202, 1258
MspR9I CCNGG 5 cut(s) 438, 564, 728, 1202, 1259
MvaI CCWGG 2 cut(s) 438, 564
MvnI CGCG 1 cut(s) 42
MwoI GCNNNNNNNGC 4 cut(s) 194, 737, 1024, 1229
NciI CCSGG 3 cut(s) 728, 1202, 1259
NcoI CCATGG 1 cut(s) 65
NdeII GATC 6 cut(s) 10, 478, 609, 616, 1144, 1152
NlaIII CATG 6 cut(s) 69, 616, 967, 1027, 1059, 1151
NmeAIII GCCGAG 1 cut(s) 717
NmuCI GTSAC 2 cut(s) 330, 550
NsiI ATGCAT 1 cut(s) 1409
NspI RCATGY 1 cut(s) 1027
PagI TCATGA 2 cut(s) 963, 1147
PciSI GCTCTTC 2 cut(s) 903, 1352
PdmI GAANNNNTTC 1 cut(s) 1196
PfeI GAWTC 7 cut(s) 47, 179, 503, 521, 533, 542, 1110
PflFI GACNNNGTC 1 cut(s) 901
PfoI TCCNGGA 1 cut(s) 1200
PkrI GCNGC 5 cut(s) 742, 871, 1051, 1054, 1101
PleI GAGTC 4 cut(s) 822, 1020, 1039, 1450
PpsI GAGTC 4 cut(s) 822, 1020, 1039, 1450
Ppu21I YACGTR 1 cut(s) 635
Psp6I CCWGG 2 cut(s) 436, 562
PspGI CCWGG 2 cut(s) 436, 562
PspPI GGNCC 2 cut(s) 133, 384
PstI CTGCAG 1 cut(s) 1054
PsuI RGATCY 1 cut(s) 616
PsyI GACNNNGTC 1 cut(s) 901
RsaI GTAC 2 cut(s) 260, 281
RsaNI GTAC 2 cut(s) 259, 280
RseI CAYNNNNRTG 3 cut(s) 70, 492, 1260
SalI GTCGAC 2 cut(s) 426, 1455
SapI GCTCTTC 2 cut(s) 903, 1352
SaqAI TTAA 5 cut(s) 399, 809, 1163, 1418, 1576
SatI GCNGC 5 cut(s) 741, 870, 1050, 1053, 1100
Sau3AI GATC 6 cut(s) 10, 478, 609, 616, 1144, 1152
Sau96I GGNCC 2 cut(s) 133, 384
SchI GAGTC 4 cut(s) 823, 1021, 1040, 1451
ScrFI CCNGG 5 cut(s) 438, 564, 728, 1202, 1259
SduI GDGCHC 2 cut(s) 341, 436
SfaNI GCATC 5 cut(s) 163, 648, 988, 1394, 1549
SfcI CTRYAG 1 cut(s) 1050
SinI GGWCC 2 cut(s) 133, 384
SmiMI CAYNNNNRTG 3 cut(s) 70, 492, 1260
SmlI CTYRAG 5 cut(s) 110, 620, 879, 1007, 1028
SmoI CTYRAG 5 cut(s) 110, 620, 879, 1007, 1028
Sse9I AATT 6 cut(s) 97, 556, 713, 784, 918, 1268
SsiI CCGC 1 cut(s) 277
SspMI CTAG 4 cut(s) 825, 1071, 1439, 1490
StyD4I CCNGG 5 cut(s) 436, 562, 726, 1200, 1257
StyI CCWWGG 2 cut(s) 65, 454
TaaI ACNGT 3 cut(s) 254, 263, 959
TaiI ACGT 3 cut(s) 433, 637, 1418
TaqI TCGA 6 cut(s) 123, 427, 978, 1127, 1368, 1456
TaqII GACCGA 1 cut(s) 77
TasI AATT 6 cut(s) 97, 556, 713, 784, 918, 1268
TfiI GAWTC 7 cut(s) 47, 179, 503, 521, 533, 542, 1110
Tru1I TTAA 5 cut(s) 399, 809, 1163, 1418, 1576
Tru9I TTAA 5 cut(s) 399, 809, 1163, 1418, 1576
TscAI CASTG 2 cut(s) 682, 1386
TseFI GTSAC 2 cut(s) 330, 550
TseI GCWGC 5 cut(s) 740, 869, 1049, 1052, 1099
Tsp45I GTSAC 2 cut(s) 330, 550
TspDTI ATGAA 1 cut(s) 867
TspGWI ACGGA 1 cut(s) 163
TspRI CASTG 2 cut(s) 682, 1386
Tth111I GACNNNGTC 1 cut(s) 901
VpaK11BI GGWCC 2 cut(s) 133, 384
XapI RAATTY 3 cut(s) 97, 713, 784
XceI RCATGY 1 cut(s) 1027
XmiI GTMKAC 2 cut(s) 427, 1456
XmnI GAANNNNTTC 1 cut(s) 1196
XspI CTAG 4 cut(s) 825, 1071, 1439, 1490
Zsp2I ATGCAT 1 cut(s) 1409
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.