Basic Information

Type: gene

Biological Identity

Species

malus_domestica

Chromosome

Chr03

Orthogroup ID

OG0005248

Physical Location & Seq

Genomic Coordinates Forward (+)

4023082 .. 4027499

Gene Structure 4418 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

MD03G1050500.v1.1.491

Sequence Viewer

CDS Protein Promoter (2k)

Length: 2076 bp

ATGGCTAATTCTTTGGTTGAGTCCAATAAGACTATGAAGAGGTTAGAATCAAGAAAGTCTCGTTCATGGTGGTGGGATAGTCACATCAGTCACAAGAACTCTAAGTGGCTTGCAGATAATCTCGAGGAGATGGATCGGAGCATCAAACGGATGTTGAAGCTGATCGAAGAGGATGGGGATTCATTTGCAAAAAAGGCTGAAATGTATTATCAAAAGAGGCCTGAATTGATTGCTCACGTTGAAGAGTTTTATCGCTTGTACCGGTCCCTGGCCGAACGTTATGATCATGTGACAGGAGAATTGCGGAAGAATGCACCCTCGGATCTCCAATCCCAGAGTTCATGCCTTTCTGACATTGGTTCAGAACTGCCATCTGCATTGCCATCTCCTGATGTCCAACCCGGAAAGCTAGGTCGTCATAAATCTGGCCCTCGTGCTGCTGGCTTTGATTTCTTCCTTGGCCCTGGTGGAAACAGCTCAGATCATTACCAGAAAGAGGGAGACGAATCATTTTCATTTACCGATTATTCTGAGCCAGAATCTGATGATTCTTCAGTCAACAATTATTCAACTCCCTTGTCAAATGGTCTTGATCAAGGGCAGACGAGAAAGATAATTGAGTTGGAAATTGAGCTTCGTGAAGTGAAGGAGAAGCTTCGGATGCAGCAGGAAGAGAATGTGGACAGTTCATTCATGGGTGCAAAACCTGATCATACCGAAGAATTTCCTGCAAAAATTGCAGAATATGAGCATGAGCTGACAAGCAGAAATGAAAAATTGCGCGAATCGGAAGAAGAGATTGCCAGGTTGAACATCAAGATCAAAAGGTACGAGTCTTCGCAACATAATAATGGTCTTAAGCTTGCACTTGAACCAGAGTCTGCACAACAGAATAATGGTCTTAAGCTTGCACTTGAACCAGAGTCTGCACAACATAATAATGGTCTTAAGCTTGCACTTGAACCAGAGCCTGCACAACATAATAATGGTCTTAAGCTTGCACTTGAACCAGAGCCTGCACAACATAATAATGGTCTTAAGCTTGCACTTGAATTTTCAAAACCGAAAGAGGCCAAGATACATGAGGGTGCAAGAGACAGGGAGATAAATGAACCGTCAGAGATTCATAAAAGGGTCAGTGGGTCGGGAGAAGTTCAAGACAGAGATAGCAAGATTGAGGCAATGGTGAAAGAGCTTAGAGCCGCAAAAGATAGACTTCAACATTCAGAAAAAGAGGTTGCAAGTTTGAGACAGCAACTTGAGAGTAATAAACCCTCTGAGGAAATCCAACAGTTGCAGGGTCAGCTTGAATCCGCTAAGAAGGATATTTCTATGTGGAAAACGAAGCTCAACACAGAGAAACGAGAGGTGTCCAAGCTGCAGGAACGAATTTCAAGGTTGAAAGGTAGTTTAATAGACCGAGATAATGAAGTCATGGATTTGAAAATCGCAGTATCTGATGCCGAGGAGAAGATTTTCCCTGAGAAGGCACAGGTTAAGGCTGAAATATCTCGATTGCAATCAGAACGGACACATTTAGAGGAGCAGCTTAAAGATTGGGAATCACGTGGTCGTTTGTTGGAGGATGAAATCAGACAGATGAAAGCTGGAAAAGCAGAAATGGAGGAGAGACTTAAAGGCAAAATCGAGCAGTTGAAGGCGGACATCCTTGAGAGAAGCAACCAAATGGAGAATTTAAACAAGACCCTTGATGCAATGAAAACAGAAAGAGATGAGCTTAGCACCAAAGCCGCTATGCTACAGGCAGAGGTGAGTTCAAGAGACGATCAGATCAATGAGATAAACAAGAATTTGCAGCAAATGCAGACGGAACATCAAGAACTGCTCAATGGGGCTGAAGGGGCGCGAAAACTGGTGGAGGAGCTGACAGAAAGAGCGAAGGAACTCGAGGAGGAGATTCAGAGGCAAAGAGTCGTGATCATGGAAGGAGCTGAAGAAAAACGAGAAGCTATAAGGCAGCTGTGCTTCTCGCTCGAACATTACAGGAACGGATACCATATGCTTCGACAAGCTTGTATGGGAAACAACAAAAGAGTTCCAGTTTTGGCAACTTAA

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0005575 GO:0005622 GO:0005623 GO:0005737 GO:0005773 GO:0005774 GO:0016020 GO:0031090 GO:0043226 GO:0043227 GO:0043229 GO:0043231 GO:0044422 GO:0044424 GO:0044437 GO:0044444 GO:0044446 GO:0044464 GO:0098588 GO:0098805

KEGG Pathways

Metabolic & Signaling

ko04530 map04530

Pfam Domains

Protein Families

KIP1

Protein Analysis

692

Amino Acids

79.39

Weight (kDa)

5.55

Isoelectric Point (pI)

62.32

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
KIP1	PF07765	22 - 95	1.7e-36	KIP1-like protein
CCDC39	PF24161	385 - 671	2.6e-07	Coiled-coil domain-containing protein 39
PUMA_CC	PF24627	398 - 468	2.2e-07	PUMA coiled-coil

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

Style Settings

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0005248)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT2G30500 AT2G30500 AT2G30500 AT5G58320 AT5G58320 AT5G58320 AT5G58320 AT5G58320
fragaria_vesca	FvH4_3g40650 FvH4_3g40650
malus_domestica	MD03G1050500.v1.1 MD11G1052600.v1.1
prunus_persica	Prupe.6G040100_v2.0.a1 Prupe.6G040100_v2.0.a1
pyrus_communis	pycom03g03990 pycom11g04290 pycom17g24080
rosa_chinensis	RchiOBHm_Chr5g0073401
rosa_laevigata	RLG00000036383
rosa_multiflora	Rmu_co8503505.1_g000001 Rmu_sc0001997.1_g000010
rosa_roxburghii	Rroxscaffold_1G00007840
rosa_rugosa	Rorug05G0423400 Rorug05G0423400
rosa_samantha	Rh5AG479900 Rh5BG501000 Rh5CG524100 Rh5DG513000
rosa_wichuraiana	Rw5G044620

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccII	CGCG	2 cut(s)	783, 1867
AciI	CCGC	5 cut(s)	304, 1203, 1314, 1661, 1752
AclI	AACGTT	1 cut(s)	277
AclWI	GGATC	2 cut(s)	141, 330
AcoI	YGGCCR	1 cut(s)	270
AcsI	RAATTY	5 cut(s)	722, 1052, 1389, 1693, 1810
AcuI	CTGAAG	3 cut(s)	537, 1878, 1974
AcvI	CACGTG	1 cut(s)	1568
AfaI	GTAC	2 cut(s)	260, 830
AfiI	CCNNNNNNNGG	3 cut(s)	268, 496, 1486
AflII	CTTAAG	5 cut(s)	857, 902, 947, 992, 1037
AgeI	ACCGGT	1 cut(s)	261
AjnI	CCWGG	3 cut(s)	267, 463, 803
Alw26I	GTCTC	6 cut(s)	63, 495, 1089, 1243, 1624, 1776
AlwI	GGATC	2 cut(s)	141, 330
AlwNI	CAGNNNCTG	6 cut(s)	542, 881, 926, 971, 1016, 1457
Ama87I	CYCGRG	2 cut(s)	122, 1907
AoxI	GGCC	5 cut(s)	218, 270, 427, 460, 1071
ApeKI	GCWGC	6 cut(s)	437, 664, 1378, 1546, 1816, 1978
ApoI	RAATTY	5 cut(s)	722, 1052, 1389, 1693, 1810
AsiGI	ACCGGT	1 cut(s)	261
Asp700I	GAANNNNTTC	2 cut(s)	723, 1475
AspLEI	GCGC	2 cut(s)	783, 1867
AspS9I	GGNCC	3 cut(s)	264, 428, 461
AsuC2I	CCSGG	1 cut(s)	402
AsuHPI	GGTGA	2 cut(s)	1198, 1783
AvaI	CYCGRG	2 cut(s)	122, 1907
AvaII	GGWCC	1 cut(s)	264
BauI	CACGAG	1 cut(s)	432
BbrPI	CACGTG	1 cut(s)	1568
BbsI	GAAGAC	1 cut(s)	828
BbvI	GCAGC	6 cut(s)	424, 676, 1365, 1558, 1828, 1990
BccI	CCATC	4 cut(s)	124, 167, 379, 391
BciT130I	CCWGG	3 cut(s)	269, 465, 805
BciVI	GTATCC	1 cut(s)	2006
BclI	TGATCA	4 cut(s)	283, 592, 709, 1938
BcnI	CCSGG	1 cut(s)	402
BcoDI	GTCTC	6 cut(s)	63, 495, 1089, 1243, 1624, 1776
BfaI	CTAG	1 cut(s)	410
BfmI	CTRYAG	2 cut(s)	1379, 1760
BfrI	CTTAAG	5 cut(s)	857, 902, 947, 992, 1037
BfuI	GTATCC	1 cut(s)	2006
BisI	GCNGC	8 cut(s)	438, 665, 1203, 1379, 1547, 1752, 1817, 1979
BlpI	GCTNAGC	1 cut(s)	1739
BlsI	GCNGC	8 cut(s)	439, 666, 1204, 1380, 1548, 1753, 1818, 1980
Bme1390I	CCNGG	4 cut(s)	269, 402, 465, 805
Bme18I	GGWCC	1 cut(s)	264
BmeT110I	CYCGRG	2 cut(s)	122, 1907
BmgT120I	GGNCC	3 cut(s)	264, 428, 461
BmiI	GGNNCC	1 cut(s)	266
BmrFI	CCNGG	4 cut(s)	269, 402, 465, 805
BmsI	GCATC	4 cut(s)	150, 651, 1450, 1702
BpiI	GAAGAC	1 cut(s)	828
Bpu1102I	GCTNAGC	1 cut(s)	1739
BpuEI	CTTGAG	2 cut(s)	1280, 1691
BpuMI	CCSGG	1 cut(s)	402
BsaAI	YACGTR	1 cut(s)	1568
BsaBI	GATNNNNATC	1 cut(s)	1519
BsaJI	CCNNGG	5 cut(s)	267, 318, 457, 463, 1464
BsaWI	WCCGGW	1 cut(s)	261
Bsc4I	CCNNNNNNNGG	3 cut(s)	268, 496, 1486
Bse118I	RCCGGY	1 cut(s)	261
Bse1I	ACTGG	2 cut(s)	1878, 2060
Bse3DI	GCAATG	3 cut(s)	377, 1188, 1722
Bse8I	GATNNNNATC	1 cut(s)	1519
BseBI	CCWGG	3 cut(s)	269, 465, 805
BseDI	CCNNGG	5 cut(s)	267, 318, 457, 463, 1464
BseGI	GGATG	5 cut(s)	156, 178, 666, 1591, 1665
BseJI	GATNNNNATC	1 cut(s)	1519
BseLI	CCNNNNNNNGG	3 cut(s)	268, 496, 1486
BseMI	GCAATG	3 cut(s)	377, 1188, 1722
BseMII	CTCAG	4 cut(s)	492, 522, 1269, 1473
BseNI	ACTGG	2 cut(s)	1878, 2060
BseRI	GAGGAG	7 cut(s)	140, 1481, 1556, 1640, 1895, 1925, 1928
BseXI	GCAGC	6 cut(s)	424, 676, 1365, 1558, 1828, 1990
BsgI	GTGCAG	4 cut(s)	867, 912, 957, 1002
Bsh1236I	CGCG	2 cut(s)	783, 1867
BshFI	GGCC	5 cut(s)	220, 272, 429, 462, 1073
BshTI	ACCGGT	1 cut(s)	261
BsiHKCI	CYCGRG	2 cut(s)	122, 1907
BsiSI	CCGG	2 cut(s)	262, 402
BslFI	GGGAC	1 cut(s)	250
BslI	CCNNNNNNNGG	3 cut(s)	268, 496, 1486
BsmAI	GTCTC	6 cut(s)	63, 495, 1089, 1243, 1624, 1776
BsmBI	CGTCTC	2 cut(s)	495, 1776
BsmFI	GGGAC	1 cut(s)	250
BsmI	GAATGC	1 cut(s)	316
BsnI	GGCC	5 cut(s)	220, 272, 429, 462, 1073
BsoBI	CYCGRG	2 cut(s)	122, 1907
Bsp1720I	GCTNAGC	1 cut(s)	1739
BspACI	CCGC	5 cut(s)	304, 1203, 1314, 1661, 1752
BspANI	GGCC	5 cut(s)	220, 272, 429, 462, 1073
BspCNI	CTCAG	4 cut(s)	491, 523, 1270, 1474
BspFNI	CGCG	2 cut(s)	783, 1867
BspLI	GGNNCC	1 cut(s)	266
BspMAI	CTGCAG	1 cut(s)	1383
BspPI	GGATC	2 cut(s)	141, 330
BspTI	CTTAAG	5 cut(s)	857, 902, 947, 992, 1037
BsrDI	GCAATG	3 cut(s)	377, 1188, 1722
BsrFI	RCCGGY	1 cut(s)	261
BsrI	ACTGG	2 cut(s)	1878, 2060
BssAI	RCCGGY	1 cut(s)	261
BssECI	CCNNGG	5 cut(s)	267, 318, 457, 463, 1464
BssSI	CACGAG	1 cut(s)	432
BssT1I	CCWWGG	1 cut(s)	457
Bst2BI	CACGAG	1 cut(s)	432
Bst2UI	CCWGG	3 cut(s)	269, 465, 805
Bst4CI	ACNGT	3 cut(s)	686, 1116, 1293
Bst6I	CTCTTC	5 cut(s)	32, 162, 237, 666, 789
BstAFI	CTTAAG	5 cut(s)	857, 902, 947, 992, 1037
BstAPI	GCANNNNNTGC	2 cut(s)	737, 1822
BstBAI	YACGTR	1 cut(s)	1568
BstC8I	GCNNGC	9 cut(s)	111, 442, 864, 909, 954, 972, 999, 1017, 1044
BstDEI	CTNAG	8 cut(s)	102, 478, 531, 1196, 1278, 1317, 1482, 1739
BstF5I	GGATG	5 cut(s)	156, 178, 666, 1591, 1665
BstFNI	CGCG	2 cut(s)	783, 1867
BstHHI	GCGC	2 cut(s)	783, 1867
BstMAI	GTCTC	6 cut(s)	63, 495, 1089, 1243, 1624, 1776
BstMWI	GCNNNNNNNGC	7 cut(s)	194, 661, 737, 1303, 1613, 1822, 1862
BstNI	CCWGG	3 cut(s)	269, 465, 805
BstSCI	CCNGG	4 cut(s)	267, 400, 463, 803
BstSFI	CTRYAG	2 cut(s)	1379, 1760
BstUI	CGCG	2 cut(s)	783, 1867
BstV1I	GCAGC	6 cut(s)	424, 676, 1365, 1558, 1828, 1990
BstV2I	GAAGAC	1 cut(s)	828
BstX2I	RGATCY	1 cut(s)	322
BstYI	RGATCY	1 cut(s)	322
BsuI	GTATCC	1 cut(s)	2006
BsuRI	GGCC	5 cut(s)	220, 272, 429, 462, 1073
BtsCI	GGATG	5 cut(s)	156, 178, 666, 1591, 1665
BtsIMutI	CAGTG	1 cut(s)	1144
Cac8I	GCNNGC	9 cut(s)	111, 442, 864, 909, 954, 972, 999, 1017, 1044
CaiI	CAGNNNCTG	6 cut(s)	542, 881, 926, 971, 1016, 1457
CfoI	GCGC	2 cut(s)	783, 1867
Cfr10I	RCCGGY	1 cut(s)	261
Cfr13I	GGNCC	3 cut(s)	264, 428, 461
Csp6I	GTAC	2 cut(s)	259, 829
CspAI	ACCGGT	1 cut(s)	261
CviAII	CATG	8 cut(s)	66, 287, 342, 694, 752, 1082, 1435, 1942
CviQI	GTAC	2 cut(s)	259, 829
DdeI	CTNAG	8 cut(s)	102, 478, 531, 1196, 1278, 1317, 1482, 1739
DraI	TTTAAA	1 cut(s)	1698
EaeI	YGGCCR	1 cut(s)	270
Eam1104I	CTCTTC	5 cut(s)	32, 162, 237, 666, 789
EarI	CTCTTC	5 cut(s)	32, 162, 237, 666, 789
EciI	GGCGGA	1 cut(s)	1676
Eco130I	CCWWGG	1 cut(s)	457
Eco147I	AGGCCT	1 cut(s)	220
Eco47I	GGWCC	1 cut(s)	264
Eco57I	CTGAAG	3 cut(s)	537, 1878, 1974
Eco72I	CACGTG	1 cut(s)	1568
Eco88I	CYCGRG	2 cut(s)	122, 1907
EcoRII	CCWGG	3 cut(s)	267, 463, 803
EcoT14I	CCWWGG	1 cut(s)	457
ErhI	CCWWGG	1 cut(s)	457
Esp3I	CGTCTC	2 cut(s)	495, 1776
FaeI	CATG	8 cut(s)	69, 290, 345, 697, 755, 1085, 1438, 1945
FalI	AAGNNNNNCTT	2 cut(s)	1200, 1232
FaqI	GGGAC	1 cut(s)	250
FatI	CATG	8 cut(s)	65, 286, 341, 693, 751, 1081, 1434, 1941
FauNDI	CATATG	1 cut(s)	2019
FbaI	TGATCA	4 cut(s)	283, 592, 709, 1938
Fnu4HI	GCNGC	8 cut(s)	438, 665, 1203, 1379, 1547, 1752, 1817, 1979
FokI	GGATG	5 cut(s)	163, 185, 673, 1598, 1652
Fsp4HI	GCNGC	8 cut(s)	438, 665, 1203, 1379, 1547, 1752, 1817, 1979
FspBI	CTAG	1 cut(s)	410
GlaI	GCGC	2 cut(s)	782, 1866
GluI	GCNGC	8 cut(s)	438, 665, 1203, 1379, 1547, 1752, 1817, 1979
HaeIII	GGCC	5 cut(s)	220, 272, 429, 462, 1073
HapII	CCGG	2 cut(s)	262, 402
HhaI	GCGC	2 cut(s)	783, 1867
Hin1II	CATG	8 cut(s)	69, 290, 345, 697, 755, 1085, 1438, 1945
Hin6I	GCGC	2 cut(s)	781, 1865
HinP1I	GCGC	2 cut(s)	781, 1865
HincII	GTYRAC	1 cut(s)	559
HindII	GTYRAC	1 cut(s)	559
HindIII	AAGCTT	7 cut(s)	653, 860, 905, 950, 995, 1040, 2031
HpaII	CCGG	2 cut(s)	262, 402
HphI	GGTGA	2 cut(s)	1198, 1783
Hpy166II	GTNNAC	2 cut(s)	559, 682
Hpy8I	GTNNAC	2 cut(s)	559, 682
HpyAV	CCTTC	7 cut(s)	640, 1315, 1480, 1651, 1853, 1894, 1940
HpyCH4III	ACNGT	3 cut(s)	686, 1116, 1293
HpyCH4IV	ACGT	3 cut(s)	237, 277, 1567
HpyF10VI	GCNNNNNNNGC	7 cut(s)	194, 661, 737, 1303, 1613, 1822, 1862
HpyF3I	CTNAG	8 cut(s)	102, 478, 531, 1196, 1278, 1317, 1482, 1739
HpySE526I	ACGT	3 cut(s)	237, 277, 1567
Hsp92II	CATG	8 cut(s)	69, 290, 345, 697, 755, 1085, 1438, 1945
HspAI	GCGC	2 cut(s)	781, 1865
Ksp22I	TGATCA	4 cut(s)	283, 592, 709, 1938
LmnI	GCTCC	4 cut(s)	138, 1543, 1882, 1949
Lsp1109I	GCAGC	6 cut(s)	424, 676, 1365, 1558, 1828, 1990
LweI	GCATC	4 cut(s)	150, 651, 1450, 1702
MaeI	CTAG	1 cut(s)	410
MaeII	ACGT	3 cut(s)	237, 277, 1567
MaeIII	GTNAC	3 cut(s)	80, 89, 289
MflI	RGATCY	1 cut(s)	322
MlyI	GAGTC	5 cut(s)	29, 842, 887, 932, 1941
MmeI	TCCRAC	4 cut(s)	421, 603, 1312, 1560
MroXI	GAANNNNTTC	2 cut(s)	723, 1475
MslI	CAYNNNNRTG	6 cut(s)	70, 849, 939, 984, 1029, 1086
MspA1I	CMGCKG	1 cut(s)	1981
MspCI	CTTAAG	5 cut(s)	857, 902, 947, 992, 1037
MspI	CCGG	2 cut(s)	262, 402
MspR9I	CCNGG	4 cut(s)	269, 402, 465, 805
Mva1269I	GAATGC	1 cut(s)	316
MvaI	CCWGG	3 cut(s)	269, 465, 805
MvnI	CGCG	2 cut(s)	783, 1867
MwoI	GCNNNNNNNGC	7 cut(s)	194, 661, 737, 1303, 1613, 1822, 1862
NciI	CCSGG	1 cut(s)	402
NdeI	CATATG	1 cut(s)	2019
NlaIII	CATG	8 cut(s)	69, 290, 345, 697, 755, 1085, 1438, 1945
NlaIV	GGNNCC	1 cut(s)	266
NmeAIII	GCCGAG	1 cut(s)	1489
NmuCI	GTSAC	3 cut(s)	80, 89, 289
PaeR7I	CTCGAG	2 cut(s)	122, 1907
PceI	AGGCCT	1 cut(s)	220
PctI	GAATGC	1 cut(s)	316
PdmI	GAANNNNTTC	2 cut(s)	723, 1475
PinAI	ACCGGT	1 cut(s)	261
PkrI	GCNGC	8 cut(s)	439, 666, 1204, 1380, 1548, 1753, 1818, 1980
PleI	GAGTC	5 cut(s)	28, 841, 886, 931, 1940
PmaCI	CACGTG	1 cut(s)	1568
PmlI	CACGTG	1 cut(s)	1568
PpsI	GAGTC	5 cut(s)	28, 841, 886, 931, 1940
Ppu21I	YACGTR	1 cut(s)	1568
Psp1406I	AACGTT	1 cut(s)	277
Psp6I	CCWGG	3 cut(s)	267, 463, 803
PspCI	CACGTG	1 cut(s)	1568
PspGI	CCWGG	3 cut(s)	267, 463, 803
PspN4I	GGNNCC	1 cut(s)	266
PspPI	GGNCC	3 cut(s)	264, 428, 461
PspXI	VCTCGAGB	1 cut(s)	1907
PstI	CTGCAG	1 cut(s)	1383
PstNI	CAGNNNCTG	6 cut(s)	542, 881, 926, 971, 1016, 1457
PsuI	RGATCY	1 cut(s)	322
PvuII	CAGCTG	1 cut(s)	1981
RsaI	GTAC	2 cut(s)	260, 830
RsaNI	GTAC	2 cut(s)	259, 829
RseI	CAYNNNNRTG	6 cut(s)	70, 849, 939, 984, 1029, 1086
SatI	GCNGC	8 cut(s)	438, 665, 1203, 1379, 1547, 1752, 1817, 1979
Sau96I	GGNCC	3 cut(s)	264, 428, 461
SchI	GAGTC	5 cut(s)	29, 842, 887, 932, 1941
ScrFI	CCNGG	4 cut(s)	269, 402, 465, 805
SfaNI	GCATC	4 cut(s)	150, 651, 1450, 1702
SfcI	CTRYAG	2 cut(s)	1379, 1760
Sfr274I	CTCGAG	2 cut(s)	122, 1907
SinI	GGWCC	1 cut(s)	264
SlaI	CTCGAG	2 cut(s)	122, 1907
SmiMI	CAYNNNNRTG	6 cut(s)	70, 849, 939, 984, 1029, 1086
SmlI	CTYRAG	9 cut(s)	122, 857, 902, 947, 992, 1037, 1259, 1670, 1907
SmoI	CTYRAG	9 cut(s)	122, 857, 902, 947, 992, 1037, 1259, 1670, 1907
SseBI	AGGCCT	1 cut(s)	220
SsiI	CCGC	5 cut(s)	304, 1203, 1314, 1661, 1752
SspMI	CTAG	1 cut(s)	410
StuI	AGGCCT	1 cut(s)	220
StyD4I	CCNGG	4 cut(s)	267, 400, 463, 803
StyI	CCWWGG	1 cut(s)	457
TaaI	ACNGT	3 cut(s)	686, 1116, 1293
TaiI	ACGT	3 cut(s)	240, 280, 1570
TaqI	TCGA	7 cut(s)	123, 165, 1513, 1647, 1908, 1995, 2026
TaqII	GACCGA	1 cut(s)	1434
TauI	GCSGC	2 cut(s)	1205, 1754
TscAI	CASTG	1 cut(s)	1144
TseFI	GTSAC	3 cut(s)	80, 89, 289
TseI	GCWGC	6 cut(s)	437, 664, 1378, 1546, 1816, 1978
Tsp45I	GTSAC	3 cut(s)	80, 89, 289
TspGWI	ACGGA	4 cut(s)	163, 1543, 1844, 2025
TspRI	CASTG	1 cut(s)	1144
Vha464I	CTTAAG	5 cut(s)	857, 902, 947, 992, 1037
VpaK11BI	GGWCC	1 cut(s)	264
XapI	RAATTY	5 cut(s)	722, 1052, 1389, 1693, 1810
XhoI	CTCGAG	2 cut(s)	122, 1907
XmnI	GAANNNNTTC	2 cut(s)	723, 1475
XspI	CTAG	1 cut(s)	410

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.