Rh5AG479900

KIP1-like protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_samantha
Chr5A
OG0005248
Physical Location & Seq
Reverse (-)
82390436 .. 82394737
4302 bp
Loading structure...
UTR
Exon/CDS
Intron
Rh5AG479900.1

Sequence Viewer

Length: 1830 bp
ATGCCTTTCAAGAAAATGACAAAACGGTCCTTGACTTCAGAGATGGACAGGTGCATCAAGCGGATGCTGAAGCTGGTTGAAGAAGATGGGGATTCATTTGCAAAAAAGGCTGAAATGTATTATCAAAAGAGGCCTGAATTGATTTCTCATGTTGAAGATTTTTATCGCTTGTACCGGTCTCTAGCCGAACGTTATGATCATGTGACAGGAGAGCTAAGGAAGAATGTGACCTCAGATCTCCAATCCATGAGCTCATGCATTTCTGACGGTGGTTCAGAACTGCCATCTGCATGGTCCTCGCCTGATCTCCAATCTCAGAGGCTGAGTCGTCGTAAATCTGGCCCTCGTGCTGCTGGCTTTGATTTCTTCCTTGGCCCCAGTGGAAACAGCTCAGATAATTACCAGAAAGAAGGGGAAGAATCATCTTCGTTAACCGATTCTGAGCCAGAATCAGATGACGATGATTCTTCAGTCAACAATTACTCAGGGGTGTTGTCAAATGGAGGTGATCAAGGGCAGCAGAAGAAGATAATTCAATTGGAAATCGAGCTTCGTGAAGCGAGGGAGAAGCTTCGGCTGCAGGAAGAGAATGCAGATAGTCCATTGAGGGGGTCAAAAAATGAGAAGTCCGAGGAATTTTCTGCAAAAATTGCAGAATATGCGCAAGAGCTGACGATTGCAAATGAAAAATTACAGGCCTCAGAGGAACAGATTTTCAGGTTGAACATTGAGCTCAAACGGTATAAATCATCAGAAAATGGCAATGCCTTTGAGGCCAGTCTTGAATCATCGGAACATAAAGAGGTCAAGAGGCATGAGAGTGAGCCAGACATAGAGGTAAATGAAGTATTTGAGACACCTAGGTCAGGAGAAGTTCAAGACCCTGATAACAAGATAGAGGCATTGGTGGAAGAGCTGAGAATCACAAAAGATAGGCTTCAGCATTCACAGAAAGAGATTGCAAATTTGAGAGATGAACTTGAGAGTAATAAAGCCTCAAGATTGAATCATGAACAGGAGAGTAATAAAGCCTCTGAGAAAATTCATCTGTTGCAGGGTCAGCTTGAATCTGCTAAGAAGGACATTGCTACTTGGAAAACAAAGCTTAACTCAGAGAAAAGAGAGGTTTCAAAGCTGCAGGAACGAATCGCAAGGTTAAAAACTAGTTTATCAGACAGGGATCATGAGGTCATGGATTTGAAAATAGCGGTATCTGATGCTGAGGAGAAGATATTCCCAGAGAAGGCACAGGTAAAGGCCGAAATATCTAGACTGTATTCGGAACGGACGGAACTGGAGGAGCAGATTAGAGACTGGGAGTCTCGAGGACGCTCTTTGGAGGATGAAATCAGAATGATTAAGGCTGGGAAAGCAGAGATGGAGGAGAGACTTACTAGCGAAATTGAGCAGTTAAAGGCGGAAATCCTTGAGATAGGCAATCACATCGGCAACTTACATAAGGCCCTTGATGCTATGAAAGCAGAAGGAGATGAGCTTAGCACAAAAGTTGTAACACTTAAAGCAGAAGTTAGTTATAGAGATGATAAGATAAATGAGATGGACATTCAGCTGCAGCAGTTGCACAAGGAGCAAGTGGAACTGGTTTCCGGGGCCGAAGGGGCATGGAAATTGGTGGAGGAGCTAACAGCAAGAACAAAAGAACTGGAAGAGGAGATTCAGAGGCAAAGAGTCATGATTTTAGAAGGAGCAGAAGAAAAGAGAGAAGCTATAAGGCAGTTGTGCTTCTCACTCGAACATTATAGGAACGGTTATCACAGGCTTCGGCAGGCTTGCATGGGACACAAGAGAGTTCCGGTTTTGGCGTCTTGA

Protein Analysis

609

Amino Acids

69.95

Weight (kDa)

5.22

Isoelectric Point (pI)

65.07

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
KIP1 PF07765 12 - 66 6.9e-24 KIP1-like protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 2 cut(s) 25, 862
Acc16I TGCGCA 1 cut(s) 663
AciI CCGC 3 cut(s) 61, 1208, 1418
AclI AACGTT 1 cut(s) 190
AclWI GGATC 1 cut(s) 1188
AcsI RAATTY 3 cut(s) 635, 964, 1041
AcuI CTGAAG 4 cut(s) 21, 89, 453, 923
AcyI GRCGYC 1 cut(s) 1823
AfaI GTAC 1 cut(s) 173
AfiI CCNNNNNNNGG 3 cut(s) 608, 866, 1243
AgeI ACCGGT 1 cut(s) 174
AhdI GACNNNNNGTC 1 cut(s) 1318
AhlI ACTAGT 1 cut(s) 1163
Alw21I GWGCWC 2 cut(s) 254, 735
Alw26I GTCTC 5 cut(s) 183, 848, 1305, 1326, 1381
AlwI GGATC 1 cut(s) 1188
AlwNI CAGNNNCTG 1 cut(s) 322
Ama87I CYCGRG 1 cut(s) 1323
AoxI GGCC 8 cut(s) 131, 340, 373, 696, 774, 1257, 1461, 1611
ApeKI GCWGC 6 cut(s) 350, 517, 577, 1135, 1570, 1573
ApoI RAATTY 3 cut(s) 635, 964, 1041
AsiGI ACCGGT 1 cut(s) 174
Asp700I GAANNNNTTC 1 cut(s) 1232
AspA2I CCTAGG 1 cut(s) 860
AspLEI GCGC 1 cut(s) 664
AspS9I GGNCC 6 cut(s) 27, 294, 341, 374, 1462, 1611
AsuC2I CCSGG 1 cut(s) 1609
AsuHPI GGTGA 1 cut(s) 518
AvaI CYCGRG 1 cut(s) 1323
AvaII GGWCC 2 cut(s) 27, 294
AvrII CCTAGG 1 cut(s) 860
BanII GRGCYC 2 cut(s) 254, 735
BauI CACGAG 1 cut(s) 345
Bbv12I GWGCWC 2 cut(s) 254, 735
BbvCI CCTCAGC 1 cut(s) 1221
BbvI GCAGC 6 cut(s) 337, 529, 564, 1122, 1557, 1585
BccI CCATC 5 cut(s) 37, 80, 292, 1372, 1552
BcgI CGANNNNNNTGC 4 cut(s) 279, 313, 1426, 1460
BclI TGATCA 2 cut(s) 196, 508
BcnI CCSGG 1 cut(s) 1609
BcoDI GTCTC 5 cut(s) 183, 848, 1305, 1326, 1381
BcuI ACTAGT 1 cut(s) 1163
BfaI CTAG 5 cut(s) 182, 861, 1164, 1269, 1395
BfmI CTRYAG 3 cut(s) 578, 1136, 1571
BglI GCCNNNNNGGC 2 cut(s) 773, 1619
BglII AGATCT 1 cut(s) 235
BisI GCNGC 6 cut(s) 351, 518, 578, 1136, 1571, 1574
BlnI CCTAGG 1 cut(s) 860
BlpI GCTNAGC 1 cut(s) 1496
BlsI GCNGC 6 cut(s) 352, 519, 579, 1137, 1572, 1575
Bme1390I CCNGG 1 cut(s) 1609
Bme18I GGWCC 2 cut(s) 27, 294
BmeRI GACNNNNNGTC 1 cut(s) 1318
BmeT110I CYCGRG 1 cut(s) 1323
BmgT120I GGNCC 6 cut(s) 27, 294, 341, 374, 1462, 1611
BmiI GGNNCC 2 cut(s) 376, 1612
BmrFI CCNGG 1 cut(s) 1609
BmrI ACTGGG 2 cut(s) 372, 1324
BmsI GCATC 4 cut(s) 54, 63, 1207, 1459
BmuI ACTGGG 2 cut(s) 372, 1324
BpmI CTGGAG 1 cut(s) 1316
Bpu10I CCTNAGC 2 cut(s) 215, 1221
Bpu1102I GCTNAGC 1 cut(s) 1496
BpuEI CTTGAG 3 cut(s) 982, 1001, 1448
BpuMI CCSGG 1 cut(s) 1609
BsaBI GATNNNNATC 1 cut(s) 162
BsaHI GRCGYC 1 cut(s) 1823
BsaI GGTCTC 1 cut(s) 183
BsaJI CCNNGG 4 cut(s) 370, 630, 860, 1608
BsaWI WCCGGW 2 cut(s) 174, 1813
Bsc4I CCNNNNNNNGG 3 cut(s) 608, 866, 1243
Bse118I RCCGGY 1 cut(s) 174
Bse1I ACTGG 6 cut(s) 378, 777, 1299, 1319, 1605, 1668
Bse3DI GCAATG 2 cut(s) 769, 1083
Bse8I GATNNNNATC 1 cut(s) 162
BseDI CCNNGG 4 cut(s) 370, 630, 860, 1608
BseGI GGATG 2 cut(s) 69, 1348
BseJI GATNNNNATC 1 cut(s) 162
BseLI CCNNNNNNNGG 3 cut(s) 608, 866, 1243
BseMI GCAATG 2 cut(s) 769, 1083
BseNI ACTGG 6 cut(s) 378, 777, 1299, 1319, 1605, 1668
BseRI GAGGAG 5 cut(s) 1238, 1313, 1397, 1652, 1685
BseXI GCAGC 6 cut(s) 337, 529, 564, 1122, 1557, 1585
BseYI CCCAGC 1 cut(s) 1364
BshFI GGCC 8 cut(s) 133, 342, 375, 698, 776, 1259, 1463, 1613
BshTI ACCGGT 1 cut(s) 174
BsiHKAI GWGCWC 2 cut(s) 254, 735
BsiHKCI CYCGRG 1 cut(s) 1323
BsiSI CCGG 3 cut(s) 175, 1608, 1814
BslFI GGGAC 1 cut(s) 1812
BslI CCNNNNNNNGG 3 cut(s) 608, 866, 1243
BsmAI GTCTC 5 cut(s) 183, 848, 1305, 1326, 1381
BsmFI GGGAC 1 cut(s) 1812
BsmI GAATGC 2 cut(s) 595, 943
BsnI GGCC 8 cut(s) 133, 342, 375, 698, 776, 1259, 1463, 1613
Bso31I GGTCTC 1 cut(s) 183
BsoBI CYCGRG 1 cut(s) 1323
Bsp1286I GDGCHC 2 cut(s) 254, 735
Bsp143I GATC 5 cut(s) 196, 235, 304, 508, 1180
Bsp1720I GCTNAGC 1 cut(s) 1496
BspACI CCGC 3 cut(s) 61, 1208, 1418
BspANI GGCC 8 cut(s) 133, 342, 375, 698, 776, 1259, 1463, 1613
BspHI TCATGA 3 cut(s) 1009, 1183, 1692
BspLI GGNNCC 2 cut(s) 376, 1612
BspMAI CTGCAG 3 cut(s) 582, 1140, 1575
BspPI GGATC 1 cut(s) 1188
BspQI GCTCTTC 1 cut(s) 906
BspTNI GGTCTC 1 cut(s) 183
BsrDI GCAATG 2 cut(s) 769, 1083
BsrFI RCCGGY 1 cut(s) 174
BsrI ACTGG 6 cut(s) 378, 777, 1299, 1319, 1605, 1668
BssAI RCCGGY 1 cut(s) 174
BssECI CCNNGG 4 cut(s) 370, 630, 860, 1608
BssMI GATC 5 cut(s) 196, 235, 304, 508, 1180
BssNI GRCGYC 1 cut(s) 1823
BssSI CACGAG 1 cut(s) 345
BssT1I CCWWGG 2 cut(s) 370, 860
Bst2BI CACGAG 1 cut(s) 345
Bst4CI ACNGT 5 cut(s) 27, 269, 741, 1275, 1769
Bst6I CTCTTC 3 cut(s) 579, 906, 1662
BstACI GRCGYC 1 cut(s) 1823
BstAPI GCANNNNNTGC 3 cut(s) 650, 659, 1579
BstC8I GCNNGC 3 cut(s) 355, 1788, 1792
BstENI CCTNNNNNAGG 1 cut(s) 864
BstF5I GGATG 2 cut(s) 69, 1348
BstHHI GCGC 1 cut(s) 664
BstKTI GATC 5 cut(s) 199, 238, 307, 511, 1183
BstMAI GTCTC 5 cut(s) 183, 848, 1305, 1326, 1381
BstMBI GATC 5 cut(s) 196, 235, 304, 508, 1180
BstSCI CCNGG 1 cut(s) 1607
BstSFI CTRYAG 3 cut(s) 578, 1136, 1571
BstV1I GCAGC 6 cut(s) 337, 529, 564, 1122, 1557, 1585
BstX2I RGATCY 1 cut(s) 235
BstXI CCANNNNNNTGG 1 cut(s) 291
BstYI RGATCY 1 cut(s) 235
BsuRI GGCC 8 cut(s) 133, 342, 375, 698, 776, 1259, 1463, 1613
BtsCI GGATG 2 cut(s) 69, 1348
BtsIMutI CAGTG 1 cut(s) 385
Cac8I GCNNGC 3 cut(s) 355, 1788, 1792
CaiI CAGNNNCTG 1 cut(s) 322
CciI TCATGA 3 cut(s) 1009, 1183, 1692
CfoI GCGC 1 cut(s) 664
Cfr10I RCCGGY 1 cut(s) 174
Cfr13I GGNCC 6 cut(s) 27, 294, 341, 374, 1462, 1611
CseI GACGC 2 cut(s) 1338, 1812
Csp6I GTAC 1 cut(s) 172
CspAI ACCGGT 1 cut(s) 174
CviQI GTAC 1 cut(s) 172
DpnI GATC 5 cut(s) 198, 237, 306, 510, 1182
DpnII GATC 5 cut(s) 196, 235, 304, 508, 1180
DrdI GACNNNNNNGTC 2 cut(s) 25, 862
DriI GACNNNNNGTC 1 cut(s) 1318
DseDI GACNNNNNNGTC 2 cut(s) 25, 862
Eam1104I CTCTTC 3 cut(s) 579, 906, 1662
Eam1105I GACNNNNNGTC 1 cut(s) 1318
EarI CTCTTC 3 cut(s) 579, 906, 1662
EciI GGCGGA 1 cut(s) 1433
Ecl136II GAGCTC 2 cut(s) 252, 733
Eco130I CCWWGG 2 cut(s) 370, 860
Eco147I AGGCCT 2 cut(s) 133, 698
Eco24I GRGCYC 2 cut(s) 254, 735
Eco31I GGTCTC 1 cut(s) 183
Eco47I GGWCC 2 cut(s) 27, 294
Eco53kI GAGCTC 2 cut(s) 252, 733
Eco57I CTGAAG 4 cut(s) 21, 89, 453, 923
Eco88I CYCGRG 1 cut(s) 1323
EcoICRI GAGCTC 2 cut(s) 252, 733
EcoNI CCTNNNNNAGG 1 cut(s) 864
EcoO109I RGGNCCY 1 cut(s) 1462
EcoT14I CCWWGG 2 cut(s) 370, 860
EcoT22I ATGCAT 1 cut(s) 260
EcoT38I GRGCYC 2 cut(s) 254, 735
ErhI CCWWGG 2 cut(s) 370, 860
FalI AAGNNNNNCTT 2 cut(s) 921, 953
FaqI GGGAC 1 cut(s) 1812
FbaI TGATCA 2 cut(s) 196, 508
Fnu4HI GCNGC 6 cut(s) 351, 518, 578, 1136, 1571, 1574
FokI GGATG 2 cut(s) 76, 1355
FriOI GRGCYC 2 cut(s) 254, 735
Fsp4HI GCNGC 6 cut(s) 351, 518, 578, 1136, 1571, 1574
FspBI CTAG 5 cut(s) 182, 861, 1164, 1269, 1395
FspI TGCGCA 1 cut(s) 663
GlaI GCGC 1 cut(s) 663
GluI GCNGC 6 cut(s) 351, 518, 578, 1136, 1571, 1574
GsaI CCCAGC 1 cut(s) 1368
GsuI CTGGAG 1 cut(s) 1316
HaeIII GGCC 8 cut(s) 133, 342, 375, 698, 776, 1259, 1463, 1613
HapII CCGG 3 cut(s) 175, 1608, 1814
HgaI GACGC 2 cut(s) 1338, 1812
HhaI GCGC 1 cut(s) 664
Hin1I GRCGYC 1 cut(s) 1823
Hin6I GCGC 1 cut(s) 662
HinP1I GCGC 1 cut(s) 662
HincII GTYRAC 2 cut(s) 432, 475
HindII GTYRAC 2 cut(s) 432, 475
HindIII AAGCTT 2 cut(s) 569, 1103
HpaI GTTAAC 1 cut(s) 432
HpaII CCGG 3 cut(s) 175, 1608, 1814
HphI GGTGA 1 cut(s) 518
Hpy166II GTNNAC 2 cut(s) 432, 475
Hpy8I GTNNAC 2 cut(s) 432, 475
Hpy99I CGWCG 1 cut(s) 333
HpyAV CCTTC 6 cut(s) 404, 1072, 1237, 1478, 1610, 1697
HpyCH4III ACNGT 5 cut(s) 27, 269, 741, 1275, 1769
HpyCH4IV ACGT 1 cut(s) 190
HpySE526I ACGT 1 cut(s) 190
Hsp92I GRCGYC 1 cut(s) 1823
HspAI GCGC 1 cut(s) 662
Ksp22I TGATCA 2 cut(s) 196, 508
KspAI GTTAAC 1 cut(s) 432
Kzo9I GATC 5 cut(s) 196, 235, 304, 508, 1180
LguI GCTCTTC 1 cut(s) 906
LmnI GCTCC 4 cut(s) 1300, 1588, 1639, 1706
Lsp1109I GCAGC 6 cut(s) 337, 529, 564, 1122, 1557, 1585
LweI GCATC 4 cut(s) 54, 63, 1207, 1459
MaeI CTAG 5 cut(s) 182, 861, 1164, 1269, 1395
MaeII ACGT 1 cut(s) 190
MaeIII GTNAC 3 cut(s) 202, 226, 1510
MalI GATC 5 cut(s) 198, 237, 306, 510, 1182
MboI GATC 5 cut(s) 196, 235, 304, 508, 1180
MfeI CAATTG 1 cut(s) 536
MflI RGATCY 1 cut(s) 235
MhlI GDGCHC 2 cut(s) 254, 735
MlyI GAGTC 3 cut(s) 334, 1328, 1698
Mph1103I ATGCAT 1 cut(s) 260
MroXI GAANNNNTTC 1 cut(s) 1232
MseI TTAA 6 cut(s) 431, 1107, 1157, 1359, 1412, 1518
MslI CAYNNNNRTG 2 cut(s) 289, 819
MspA1I CMGCKG 1 cut(s) 1570
MspI CCGG 3 cut(s) 175, 1608, 1814
MspR9I CCNGG 1 cut(s) 1609
MunI CAATTG 1 cut(s) 536
Mva1269I GAATGC 2 cut(s) 595, 943
NciI CCSGG 1 cut(s) 1609
NdeII GATC 5 cut(s) 196, 235, 304, 508, 1180
NlaIV GGNNCC 2 cut(s) 376, 1612
NmuCI GTSAC 2 cut(s) 202, 226
NsbI TGCGCA 1 cut(s) 663
NsiI ATGCAT 1 cut(s) 260
PaeR7I CTCGAG 1 cut(s) 1323
PagI TCATGA 3 cut(s) 1009, 1183, 1692
PceI AGGCCT 2 cut(s) 133, 698
PciSI GCTCTTC 1 cut(s) 906
PctI GAATGC 2 cut(s) 595, 943
PdmI GAANNNNTTC 1 cut(s) 1232
PinAI ACCGGT 1 cut(s) 174
PkrI GCNGC 6 cut(s) 352, 519, 579, 1137, 1572, 1575
PleI GAGTC 3 cut(s) 333, 1327, 1697
PpsI GAGTC 3 cut(s) 333, 1327, 1697
Psp124BI GAGCTC 2 cut(s) 254, 735
Psp1406I AACGTT 1 cut(s) 190
PspFI CCCAGC 1 cut(s) 1364
PspN4I GGNNCC 2 cut(s) 376, 1612
PspPI GGNCC 6 cut(s) 27, 294, 341, 374, 1462, 1611
PstI CTGCAG 3 cut(s) 582, 1140, 1575
PstNI CAGNNNCTG 1 cut(s) 322
PsuI RGATCY 1 cut(s) 235
PvuII CAGCTG 1 cut(s) 1570
RsaI GTAC 1 cut(s) 173
RsaNI GTAC 1 cut(s) 172
RseI CAYNNNNRTG 2 cut(s) 289, 819
SacI GAGCTC 2 cut(s) 254, 735
SapI GCTCTTC 1 cut(s) 906
SaqAI TTAA 6 cut(s) 431, 1107, 1157, 1359, 1412, 1518
SatI GCNGC 6 cut(s) 351, 518, 578, 1136, 1571, 1574
Sau3AI GATC 5 cut(s) 196, 235, 304, 508, 1180
Sau96I GGNCC 6 cut(s) 27, 294, 341, 374, 1462, 1611
SchI GAGTC 3 cut(s) 334, 1328, 1698
ScrFI CCNGG 1 cut(s) 1609
SduI GDGCHC 2 cut(s) 254, 735
SfaNI GCATC 4 cut(s) 54, 63, 1207, 1459
SfcI CTRYAG 3 cut(s) 578, 1136, 1571
Sfr274I CTCGAG 1 cut(s) 1323
SinI GGWCC 2 cut(s) 27, 294
SlaI CTCGAG 1 cut(s) 1323
SmiMI CAYNNNNRTG 2 cut(s) 289, 819
SmlI CTYRAG 4 cut(s) 980, 997, 1323, 1427
SmoI CTYRAG 4 cut(s) 980, 997, 1323, 1427
SpeI ACTAGT 1 cut(s) 1163
SseBI AGGCCT 2 cut(s) 133, 698
SsiI CCGC 3 cut(s) 61, 1208, 1418
SspMI CTAG 5 cut(s) 182, 861, 1164, 1269, 1395
SstI GAGCTC 2 cut(s) 254, 735
StuI AGGCCT 2 cut(s) 133, 698
StyD4I CCNGG 1 cut(s) 1607
StyI CCWWGG 2 cut(s) 370, 860
TaaI ACNGT 5 cut(s) 27, 269, 741, 1275, 1769
TaiI ACGT 1 cut(s) 193
TaqI TCGA 3 cut(s) 546, 1324, 1752
Tru1I TTAA 6 cut(s) 431, 1107, 1157, 1359, 1412, 1518
Tru9I TTAA 6 cut(s) 431, 1107, 1157, 1359, 1412, 1518
TscAI CASTG 1 cut(s) 385
TseFI GTSAC 2 cut(s) 202, 226
TseI GCWGC 6 cut(s) 350, 517, 577, 1135, 1570, 1573
Tsp45I GTSAC 2 cut(s) 202, 226
TspDTI ATGAA 8 cut(s) 84, 699, 858, 990, 1026, 1034, 1359, 1490
TspGWI ACGGA 2 cut(s) 1300, 1304
TspRI CASTG 1 cut(s) 385
VpaK11BI GGWCC 2 cut(s) 27, 294
XagI CCTNNNNNAGG 1 cut(s) 864
XapI RAATTY 3 cut(s) 635, 964, 1041
XbaI TCTAGA 1 cut(s) 1268
XhoI CTCGAG 1 cut(s) 1323
XmaJI CCTAGG 1 cut(s) 860
XmnI GAANNNNTTC 1 cut(s) 1232
XspI CTAG 5 cut(s) 182, 861, 1164, 1269, 1395
Zsp2I ATGCAT 1 cut(s) 260
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.