AT5G58930

UPF0503 protein At3g09070

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010196
Physical Location & Seq
Reverse (-)
23794286 .. 23796461
2176 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G58930.1

Sequence Viewer

Length: 1566 bp
ATGACCCACCAAACCCATCAGCGTCGTCGCCGTCGTCACTCCGCCGTCTGTCACCGTCATCCTTCTTCCAAACCCACCACTGGTTTCTGTGCCACCTGTCTCCGTGAACGTCTCTCTACCATCGAAGCTCTATCTTCCTCTGTCTCAGCTTCCACTGAGCTCCGCCGTGTTCGCTCCTACTCTGTTCGTGACGCATCCGCTTCTGTTTTGGACCAGCCACGACGCAGATCGTGTGATGTTAGGTCTAATCACGACGACGATGATGATGATGAGCTGCTAAAGAGCTCGATTCGATTCCCCATTGTACCGGATTTGATAGAGGATGAAGAAGAAGAAGATGATGAGGGTAAGAAGCTCGTTGAAGAAGAAATCGAAGATGGAGAGCAGAAGACGATGAAGGAGTTAATAGATCTGGAATCGAGAAATCAACAACTGAAGAACAACGGCAAAGATTCGGTTTTTAGCAGAACACTCAGGAAGTTTTCACTCAAACATCATCGAAAAATTCCAGATTCCGGGAACAGTCTTGGTCGTCGTTCATGTGATGTAGATCCTAGGTTGTCTCTTGACGCAGGTCGAGTCTCTTTCGACGAGCCTAGAGCTTCATGGGATGGATGTTTGATTGGAAAGACATATCCAAAGCTGATACCTTTGTCCTCTGTGACTGAAGACGTTAAAGCCTCGCCGGAGAAGATAACCGGTGAGAAGGTAGAGGAAGATGAGAAGAACAATCCAGGTGGGACAGCTCAAACGAGGGATTACTACTTGGATTCACGAAGGAGAAGAAGCTTTGATCGATCATCGAGACATGGTTTGCTTGAAGTTGATGAATTGAAAGCTATTTCTAATGCTAAGGTATCACCTGAAACTGTTGGTTTGTTTCATGGAGCGAAGTTGCTTGTTACTGAGAGGGAACTTAGGGATTCGAATTGGTATTCTATTAAGAATTACAAACCGGAGAGCTTGGAGTTAGGTTCTAAAGGTGTTGGTTGTGTTGCTGCTGGTGAGGTGAAGAAGCAGGATGGTTTTGGGTTGAAGAAGAGCGGAAAGAACTGGTCTAAAGGATGGAACTTTTGGGGATTGATTCAGAGGAAAACTGATGTGGCGAAGAACGAAATGAAAACCGAGCAAAGTTTGAAACTTGGAGGAAATACTATGGAGGGTTCTCTTGCTGAGTCTTTGTTGAAGCTTAGGAGAGTGGCTAAAGGAGAAACCAATGGTGATGTCAGTGAGAAACTCATAAGAAGTTACAGCGTTAGTGCGAGAAAGTCTTGTGATGGTATGTTACGTGGTGCTTCTATTGTTAATGGCTTTGAAGGTGGAAGAAGCTCTTGTGATGGTCTATTTCATGGCTCTATAACTGGTGTTGAGACTGGAAGAAGAAGCTTGTGTGAAGACGGAATGTTTCATGGCGTTGAAGGCAAACGGAATCATCTTCTCCAGAGTGATGACAAGCTTGGTACTTACTCGCCTGACAACCTCAGAAATGGTATGGTTAGATTCTACTTGACGCCATTAAACAGCCATATGACAAGCAAGTCCGGTAAAAGTAGGCTGATGAATTAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF740

Protein Analysis

521

Amino Acids

58.37

Weight (kDa)

8.89

Isoelectric Point (pI)

58.4

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF740 PF05340 8 - 50 2.1e-08 Protein of unknown function (DUF740)
DUF740 PF05340 175 - 508 3e-63 Protein of unknown function (DUF740)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 1537
Acc36I ACCTGC 1 cut(s) 563
AccBSI CCGCTC 1 cut(s) 1044
AciI CCGC 4 cut(s) 42, 163, 198, 1044
AclWI GGATC 1 cut(s) 545
AcsI RAATTY 1 cut(s) 504
AcuI CTGAAG 2 cut(s) 455, 687
AcyI GRCGYC 1 cut(s) 1511
AfaI GTAC 2 cut(s) 306, 1462
AfiI CCNNNNNNNGG 2 cut(s) 80, 515
AgeI ACCGGT 1 cut(s) 698
AgsI TTSAA 8 cut(s) 362, 821, 835, 1036, 1138, 1186, 1316, 1418
AjnI CCWGG 1 cut(s) 733
Alw21I GWGCWC 2 cut(s) 162, 287
Alw26I GTCTC 7 cut(s) 104, 116, 148, 567, 586, 799, 1364
AlwI GGATC 1 cut(s) 545
ApeKI GCWGC 2 cut(s) 274, 998
ApoI RAATTY 1 cut(s) 504
AsiGI ACCGGT 1 cut(s) 698
AspA2I CCTAGG 1 cut(s) 554
AspS9I GGNCC 1 cut(s) 211
AsuC2I CCSGG 1 cut(s) 517
AsuHPI GGTGA 6 cut(s) 44, 713, 852, 1016, 1021, 1232
AsuII TTCGAA 1 cut(s) 926
AvaII GGWCC 1 cut(s) 211
AvrII CCTAGG 1 cut(s) 554
BaeI ACNNNNGTAYC 2 cut(s) 638, 671
BanII GRGCYC 2 cut(s) 162, 287
BbsI GAAGAC 3 cut(s) 395, 675, 1401
Bbv12I GWGCWC 2 cut(s) 162, 287
BbvI GCAGC 2 cut(s) 261, 985
BccI CCATC 8 cut(s) 24, 128, 371, 605, 1016, 1059, 1271, 1331
BceAI ACGGC 4 cut(s) 15, 29, 150, 460
BciT130I CCWGG 1 cut(s) 735
BcnI CCSGG 1 cut(s) 517
BcoDI GTCTC 7 cut(s) 104, 116, 148, 567, 586, 799, 1364
BfaI CTAG 2 cut(s) 555, 597
BfuAI ACCTGC 1 cut(s) 563
BglII AGATCT 1 cut(s) 409
BisI GCNGC 2 cut(s) 275, 999
BlnI CCTAGG 1 cut(s) 554
BlsI GCNGC 2 cut(s) 276, 1000
Bme1390I CCNGG 2 cut(s) 517, 735
Bme18I GGWCC 1 cut(s) 211
BmgT120I GGNCC 1 cut(s) 211
BmrFI CCNGG 2 cut(s) 517, 735
BmsI GCATC 1 cut(s) 203
BoxI GACNNNNGTC 1 cut(s) 573
BpiI GAAGAC 3 cut(s) 395, 675, 1401
BpmI CTGGAG 1 cut(s) 1424
Bpu10I CCTNAGC 2 cut(s) 852, 1190
Bpu14I TTCGAA 1 cut(s) 926
BpuMI CCSGG 1 cut(s) 517
Bsa29I ATCGAT 1 cut(s) 796
BsaAI YACGTR 1 cut(s) 1289
BsaBI GATNNNNATC 1 cut(s) 549
BsaHI GRCGYC 1 cut(s) 1511
BsaJI CCNNGG 1 cut(s) 554
BsaWI WCCGGW 4 cut(s) 307, 698, 955, 1541
Bsc4I CCNNNNNNNGG 2 cut(s) 80, 515
Bse118I RCCGGY 1 cut(s) 698
Bse1I ACTGG 4 cut(s) 85, 1058, 1366, 1378
Bse8I GATNNNNATC 1 cut(s) 549
BseBI CCWGG 1 cut(s) 735
BseCI ATCGAT 1 cut(s) 796
BseDI CCNNGG 1 cut(s) 554
BseGI GGATG 7 cut(s) 58, 194, 328, 616, 620, 1027, 1070
BseJI GATNNNNATC 1 cut(s) 549
BseLI CCNNNNNNNGG 2 cut(s) 80, 515
BseMII CTCAG 6 cut(s) 147, 159, 487, 897, 1164, 1495
BseNI ACTGG 4 cut(s) 85, 1058, 1366, 1378
BseXI GCAGC 2 cut(s) 261, 985
BshTI ACCGGT 1 cut(s) 698
BshVI ATCGAT 1 cut(s) 796
BsiHKAI GWGCWC 2 cut(s) 162, 287
BsiSI CCGG 6 cut(s) 308, 516, 686, 699, 956, 1542
BslFI GGGAC 1 cut(s) 754
BslI CCNNNNNNNGG 2 cut(s) 80, 515
BsmAI GTCTC 7 cut(s) 104, 116, 148, 567, 586, 799, 1364
BsmBI CGTCTC 1 cut(s) 116
BsmFI GGGAC 1 cut(s) 754
Bsp119I TTCGAA 1 cut(s) 926
Bsp1286I GDGCHC 2 cut(s) 162, 287
Bsp143I GATC 5 cut(s) 227, 409, 550, 793, 797
BspACI CCGC 4 cut(s) 42, 163, 198, 1044
BspCNI CTCAG 6 cut(s) 148, 158, 486, 898, 1165, 1494
BspDI ATCGAT 1 cut(s) 796
BspMI ACCTGC 1 cut(s) 563
BspPI GGATC 1 cut(s) 545
BspQI GCTCTTC 1 cut(s) 1034
BspT104I TTCGAA 1 cut(s) 926
BsrBI CCGCTC 1 cut(s) 1044
BsrFI RCCGGY 1 cut(s) 698
BsrI ACTGG 4 cut(s) 85, 1058, 1366, 1378
BssAI RCCGGY 1 cut(s) 698
BssECI CCNNGG 1 cut(s) 554
BssMI GATC 5 cut(s) 227, 409, 550, 793, 797
BssNI GRCGYC 1 cut(s) 1511
BssT1I CCWWGG 1 cut(s) 554
Bst2UI CCWGG 1 cut(s) 735
Bst4CI ACNGT 3 cut(s) 56, 524, 871
Bst6I CTCTTC 1 cut(s) 1034
BstACI GRCGYC 1 cut(s) 1511
BstBAI YACGTR 1 cut(s) 1289
BstBI TTCGAA 1 cut(s) 926
BstDEI CTNAG 9 cut(s) 145, 156, 473, 852, 906, 917, 1173, 1190, 1481
BstF5I GGATG 7 cut(s) 58, 194, 328, 616, 620, 1027, 1070
BstKTI GATC 5 cut(s) 230, 412, 553, 796, 800
BstMAI GTCTC 7 cut(s) 104, 116, 148, 567, 586, 799, 1364
BstMBI GATC 5 cut(s) 227, 409, 550, 793, 797
BstMWI GCNNNNNNNGC 2 cut(s) 171, 1419
BstNI CCWGG 1 cut(s) 735
BstPAI GACNNNNGTC 1 cut(s) 573
BstSCI CCNGG 2 cut(s) 515, 733
BstV1I GCAGC 2 cut(s) 261, 985
BstV2I GAAGAC 3 cut(s) 395, 675, 1401
BstX2I RGATCY 2 cut(s) 409, 550
BstYI RGATCY 2 cut(s) 409, 550
Bsu15I ATCGAT 1 cut(s) 796
BsuTUI ATCGAT 1 cut(s) 796
BtsCI GGATG 7 cut(s) 58, 194, 328, 616, 620, 1027, 1070
BtsIMutI CAGTG 3 cut(s) 78, 153, 1234
BveI ACCTGC 1 cut(s) 563
Cfr10I RCCGGY 1 cut(s) 698
Cfr13I GGNCC 1 cut(s) 211
ClaI ATCGAT 1 cut(s) 796
CseI GACGC 5 cut(s) 11, 200, 231, 578, 1519
Csp6I GTAC 2 cut(s) 305, 1461
CspAI ACCGGT 1 cut(s) 698
CspCI CAANNNNNGTGG 2 cut(s) 718, 753
CviAII CATG 6 cut(s) 540, 606, 809, 884, 1349, 1409
CviQI GTAC 2 cut(s) 305, 1461
DdeI CTNAG 9 cut(s) 145, 156, 473, 852, 906, 917, 1173, 1190, 1481
DpnI GATC 5 cut(s) 229, 411, 552, 795, 799
DpnII GATC 5 cut(s) 227, 409, 550, 793, 797
DrdI GACNNNNNNGTC 1 cut(s) 1537
DseDI GACNNNNNNGTC 1 cut(s) 1537
Eam1104I CTCTTC 1 cut(s) 1034
EarI CTCTTC 1 cut(s) 1034
EciI GGCGGA 2 cut(s) 31, 152
Ecl136II GAGCTC 2 cut(s) 160, 285
Eco130I CCWWGG 1 cut(s) 554
Eco24I GRGCYC 2 cut(s) 162, 287
Eco47I GGWCC 1 cut(s) 211
Eco53kI GAGCTC 2 cut(s) 160, 285
Eco57I CTGAAG 2 cut(s) 455, 687
EcoICRI GAGCTC 2 cut(s) 160, 285
EcoRII CCWGG 1 cut(s) 733
EcoT14I CCWWGG 1 cut(s) 554
EcoT38I GRGCYC 2 cut(s) 162, 287
ErhI CCWWGG 1 cut(s) 554
Esp3I CGTCTC 1 cut(s) 116
FaeI CATG 6 cut(s) 543, 609, 812, 887, 1352, 1412
FalI AAGNNNNNCTT 2 cut(s) 1315, 1347
FaqI GGGAC 1 cut(s) 754
FatI CATG 6 cut(s) 539, 605, 808, 883, 1348, 1408
FauNDI CATATG 1 cut(s) 1527
Fnu4HI GCNGC 2 cut(s) 275, 999
FokI GGATG 7 cut(s) 45, 181, 335, 623, 627, 1034, 1077
FriOI GRGCYC 2 cut(s) 162, 287
Fsp4HI GCNGC 2 cut(s) 275, 999
FspBI CTAG 2 cut(s) 555, 597
GluI GCNGC 2 cut(s) 275, 999
GsuI CTGGAG 1 cut(s) 1424
HapII CCGG 6 cut(s) 308, 516, 686, 699, 956, 1542
HgaI GACGC 5 cut(s) 11, 200, 231, 578, 1519
Hin1I GRCGYC 1 cut(s) 1511
Hin1II CATG 6 cut(s) 543, 609, 812, 887, 1352, 1412
HindIII AAGCTT 4 cut(s) 787, 1187, 1384, 1454
HpaII CCGG 6 cut(s) 308, 516, 686, 699, 956, 1542
HphI GGTGA 6 cut(s) 44, 713, 852, 1016, 1021, 1232
Hpy166II GTNNAC 1 cut(s) 107
Hpy188I TCNGA 2 cut(s) 1089, 1484
Hpy8I GTNNAC 1 cut(s) 107
Hpy99I CGWCG 8 cut(s) 27, 30, 36, 225, 257, 260, 537, 593
HpyAV CCTTC 6 cut(s) 72, 391, 700, 771, 1310, 1412
HpyCH4III ACNGT 3 cut(s) 56, 524, 871
HpyCH4IV ACGT 3 cut(s) 109, 672, 1288
HpyF10VI GCNNNNNNNGC 2 cut(s) 171, 1419
HpyF3I CTNAG 9 cut(s) 145, 156, 473, 852, 906, 917, 1173, 1190, 1481
HpySE526I ACGT 3 cut(s) 109, 672, 1288
Hsp92I GRCGYC 1 cut(s) 1511
Hsp92II CATG 6 cut(s) 543, 609, 812, 887, 1352, 1412
Kzo9I GATC 5 cut(s) 227, 409, 550, 793, 797
LguI GCTCTTC 1 cut(s) 1034
LmnI GCTCC 3 cut(s) 165, 179, 887
Lsp1109I GCAGC 2 cut(s) 261, 985
LweI GCATC 1 cut(s) 203
MaeI CTAG 2 cut(s) 555, 597
MaeII ACGT 3 cut(s) 109, 672, 1288
MaeIII GTNAC 7 cut(s) 35, 50, 188, 661, 901, 1247, 1284
MalI GATC 5 cut(s) 229, 411, 552, 795, 799
MbiI CCGCTC 1 cut(s) 1044
MboI GATC 5 cut(s) 227, 409, 550, 793, 797
MflI RGATCY 2 cut(s) 409, 550
MhlI GDGCHC 2 cut(s) 162, 287
MluCI AATT 5 cut(s) 504, 830, 928, 946, 1561
MlyI GAGTC 2 cut(s) 588, 1184
MseI TTAA 5 cut(s) 404, 675, 942, 1305, 1517
MspI CCGG 6 cut(s) 308, 516, 686, 699, 956, 1542
MspR9I CCNGG 2 cut(s) 517, 735
MvaI CCWGG 1 cut(s) 735
MwoI GCNNNNNNNGC 2 cut(s) 171, 1419
NciI CCSGG 1 cut(s) 517
NdeI CATATG 1 cut(s) 1527
NdeII GATC 5 cut(s) 227, 409, 550, 793, 797
NlaIII CATG 6 cut(s) 543, 609, 812, 887, 1352, 1412
NmuCI GTSAC 4 cut(s) 35, 50, 188, 661
NspV TTCGAA 1 cut(s) 926
PciSI GCTCTTC 1 cut(s) 1034
PcsI WCGNNNNNNNCGW 1 cut(s) 31
PfoI TCCNGGA 1 cut(s) 515
PinAI ACCGGT 1 cut(s) 698
PkrI GCNGC 2 cut(s) 276, 1000
PleI GAGTC 2 cut(s) 587, 1183
PpsI GAGTC 2 cut(s) 587, 1183
Ppu21I YACGTR 1 cut(s) 1289
PshAI GACNNNNGTC 1 cut(s) 573
Psp124BI GAGCTC 2 cut(s) 162, 287
Psp6I CCWGG 1 cut(s) 733
PspGI CCWGG 1 cut(s) 733
PspPI GGNCC 1 cut(s) 211
PsuI RGATCY 2 cut(s) 409, 550
RsaI GTAC 2 cut(s) 306, 1462
RsaNI GTAC 2 cut(s) 305, 1461
SacI GAGCTC 2 cut(s) 162, 287
SapI GCTCTTC 1 cut(s) 1034
SaqAI TTAA 5 cut(s) 404, 675, 942, 1305, 1517
SatI GCNGC 2 cut(s) 275, 999
Sau3AI GATC 5 cut(s) 227, 409, 550, 793, 797
Sau96I GGNCC 1 cut(s) 211
SchI GAGTC 2 cut(s) 588, 1184
ScrFI CCNGG 2 cut(s) 517, 735
SduI GDGCHC 2 cut(s) 162, 287
SfaNI GCATC 1 cut(s) 203
SfuI TTCGAA 1 cut(s) 926
SinI GGWCC 1 cut(s) 211
Sse9I AATT 5 cut(s) 504, 830, 928, 946, 1561
SsiI CCGC 4 cut(s) 42, 163, 198, 1044
SspMI CTAG 2 cut(s) 555, 597
SstI GAGCTC 2 cut(s) 162, 287
StyD4I CCNGG 2 cut(s) 515, 733
StyI CCWWGG 1 cut(s) 554
TaaI ACNGT 3 cut(s) 56, 524, 871
TaiI ACGT 3 cut(s) 112, 675, 1291
TasI AATT 5 cut(s) 504, 830, 928, 946, 1561
Tru1I TTAA 5 cut(s) 404, 675, 942, 1305, 1517
Tru9I TTAA 5 cut(s) 404, 675, 942, 1305, 1517
TscAI CASTG 3 cut(s) 85, 160, 1234
TseFI GTSAC 4 cut(s) 35, 50, 188, 661
TseI GCWGC 2 cut(s) 274, 998
Tsp45I GTSAC 4 cut(s) 35, 50, 188, 661
TspDTI ATGAA 9 cut(s) 339, 410, 528, 594, 843, 872, 1133, 1337, 1397
TspGWI ACGGA 3 cut(s) 92, 1413, 1441
TspRI CASTG 3 cut(s) 85, 160, 1234
VpaK11BI GGWCC 1 cut(s) 211
XapI RAATTY 1 cut(s) 504
XmaJI CCTAGG 1 cut(s) 554
XspI CTAG 2 cut(s) 555, 597
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.