pycom05g14380

UPF0503 protein At3g09070, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr5
OG0010196
Physical Location & Seq
Forward (+)
17926256 .. 17926696
441 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom05g14380.1

Sequence Viewer

Length: 441 bp
ATGGAGTTGATAGAGCTGGAATGGGAGAAGAAGAAGGGTGCGGGTAGGGATTTGAAGGACATTGCAGGGACATTTTGGGGGACGACGTCGTTTTTGAGCAGGAAGGTAAGGCAATGGCGGCGGAAGCAAAAGATGAAGAAGGTTGAGAATGAGGGTTTCGTGGCGGAGAAGCGGCGGGAGACCCAGTCAGATGTCAATGGACGAGCCTCAGGATTCTTGGGACGGGTATTTGGGTCCGAGAGCCGCCCAGCCGAGGCTGACCCGGATGTTTTTGGACGGTTGTGGAAGAAAACTGGGAGGGAGGTGGGGTGGGGGTGTTGCGGGGGAGGTGGGGGTGGGGTGGGAGGGGAGGTGAAGAGAGTGGACGAGAGGAGGCGGTCGGGGGGTGGGTTTCTAGGTTTTCAGTTTTTTTTTTATTATTATTATTATTTTTTTAAATAG
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF740

Protein Analysis

147

Amino Acids

16.72

Weight (kDa)

9.87

Isoelectric Point (pI)

53.7

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF740 PF05340 2 - 57 7.4e-14 Protein of unknown function (DUF740)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AatII GACGTC 1 cut(s) 89
AciI CCGC 9 cut(s) 41, 118, 121, 164, 172, 175, 244, 321, 376
AcyI GRCGYC 1 cut(s) 86
AfiI CCNNNNNNNGG 1 cut(s) 253
AgsI TTSAA 1 cut(s) 55
AluBI AGCT 1 cut(s) 16
AluI AGCT 1 cut(s) 16
Alw26I GTCTC 1 cut(s) 173
ArsI GACNNNNNNTTYG 2 cut(s) 76, 108
AspS9I GGNCC 1 cut(s) 234
AsuC2I CCSGG 1 cut(s) 263
AsuHPI GGTGA 1 cut(s) 364
AvaII GGWCC 1 cut(s) 234
AxyI CCTNAGG 1 cut(s) 208
BcnI CCSGG 1 cut(s) 263
BcoDI GTCTC 1 cut(s) 173
BfaI CTAG 1 cut(s) 395
BisI GCNGC 3 cut(s) 119, 173, 244
BlsI GCNGC 3 cut(s) 120, 174, 245
Bme1390I CCNGG 1 cut(s) 263
Bme18I GGWCC 1 cut(s) 234
BmgT120I GGNCC 1 cut(s) 234
BmiI GGNNCC 1 cut(s) 235
BmrFI CCNGG 1 cut(s) 263
BmrI ACTGGG 2 cut(s) 178, 303
BmuI ACTGGG 2 cut(s) 178, 303
BpuMI CCSGG 1 cut(s) 263
BsaHI GRCGYC 1 cut(s) 86
BsaI GGTCTC 1 cut(s) 173
BsaJI CCNNGG 1 cut(s) 252
BsaXI ACNNNNNCTCC 6 cut(s) 170, 200, 293, 323, 336, 366
Bsc4I CCNNNNNNNGG 1 cut(s) 253
Bse1I ACTGG 2 cut(s) 184, 298
Bse21I CCTNAGG 1 cut(s) 208
Bse3DI GCAATG 2 cut(s) 60, 119
BseDI CCNNGG 1 cut(s) 252
BseGI GGATG 1 cut(s) 271
BseLI CCNNNNNNNGG 1 cut(s) 253
BseMI GCAATG 2 cut(s) 60, 119
BseMII CTCAG 1 cut(s) 222
BseNI ACTGG 2 cut(s) 184, 298
BseRI GAGGAG 1 cut(s) 385
BseYI CCCAGC 1 cut(s) 247
Bsh1285I CGRYCG 1 cut(s) 380
BsiEI CGRYCG 1 cut(s) 380
BsiSI CCGG 1 cut(s) 263
BslFI GGGAC 3 cut(s) 82, 94, 234
BslI CCNNNNNNNGG 1 cut(s) 253
BsmAI GTCTC 1 cut(s) 173
BsmFI GGGAC 3 cut(s) 82, 94, 234
Bso31I GGTCTC 1 cut(s) 173
BspACI CCGC 9 cut(s) 41, 118, 121, 164, 172, 175, 244, 321, 376
BspCNI CTCAG 1 cut(s) 221
BspLI GGNNCC 1 cut(s) 235
BspTNI GGTCTC 1 cut(s) 173
BsrDI GCAATG 2 cut(s) 60, 119
BsrI ACTGG 2 cut(s) 184, 298
BssECI CCNNGG 1 cut(s) 252
BssNI GRCGYC 1 cut(s) 86
Bst4CI ACNGT 1 cut(s) 279
Bst6I CTCTTC 1 cut(s) 350
BstACI GRCGYC 1 cut(s) 86
BstDEI CTNAG 1 cut(s) 208
BstF5I GGATG 1 cut(s) 271
BstMAI GTCTC 1 cut(s) 173
BstMCI CGRYCG 1 cut(s) 380
BstMWI GCNNNNNNNGC 2 cut(s) 118, 124
BstSCI CCNGG 1 cut(s) 261
Bsu36I CCTNAGG 1 cut(s) 208
BtsCI GGATG 1 cut(s) 271
Cfr13I GGNCC 1 cut(s) 234
CviJI RGCY 5 cut(s) 16, 206, 243, 251, 257
CviKI_1 RGCY 5 cut(s) 16, 206, 243, 251, 257
DdeI CTNAG 1 cut(s) 208
DraI TTTAAA 1 cut(s) 436
Eam1104I CTCTTC 1 cut(s) 350
EarI CTCTTC 1 cut(s) 350
EciI GGCGGA 2 cut(s) 136, 179
Eco31I GGTCTC 1 cut(s) 173
Eco47I GGWCC 1 cut(s) 234
Eco81I CCTNAGG 1 cut(s) 208
FaqI GGGAC 3 cut(s) 82, 94, 234
FauI CCCGC 3 cut(s) 34, 168, 314
Fnu4HI GCNGC 3 cut(s) 119, 173, 244
FokI GGATG 1 cut(s) 278
Fsp4HI GCNGC 3 cut(s) 119, 173, 244
FspBI CTAG 1 cut(s) 395
GluI GCNGC 3 cut(s) 119, 173, 244
GsaI CCCAGC 1 cut(s) 251
HapII CCGG 1 cut(s) 263
Hin1I GRCGYC 1 cut(s) 86
HinfI GANTC 1 cut(s) 213
HpaII CCGG 1 cut(s) 263
HphI GGTGA 1 cut(s) 364
Hpy166II GTNNAC 1 cut(s) 364
Hpy188I TCNGA 2 cut(s) 190, 238
Hpy188III TCNNGA 1 cut(s) 210
Hpy8I GTNNAC 1 cut(s) 364
Hpy99I CGWCG 2 cut(s) 88, 91
HpyAV CCTTC 4 cut(s) 28, 49, 97, 133
HpyCH4III ACNGT 1 cut(s) 279
HpyCH4IV ACGT 1 cut(s) 86
HpyCH4V TGCA 1 cut(s) 65
HpyF10VI GCNNNNNNNGC 2 cut(s) 118, 124
HpyF3I CTNAG 1 cut(s) 208
HpySE526I ACGT 1 cut(s) 86
Hsp92I GRCGYC 1 cut(s) 86
LpnPI CCDG 8 cut(s) 2, 51, 85, 195, 197, 261, 276, 279
MaeI CTAG 1 cut(s) 395
MaeII ACGT 1 cut(s) 86
MboII GAAGA 5 cut(s) 40, 43, 148, 298, 367
MseI TTAA 1 cut(s) 435
MspI CCGG 1 cut(s) 263
MspR9I CCNGG 1 cut(s) 263
MwoI GCNNNNNNNGC 2 cut(s) 118, 124
NciI CCSGG 1 cut(s) 263
NlaIV GGNNCC 1 cut(s) 235
NmeAIII GCCGAG 1 cut(s) 277
PfeI GAWTC 1 cut(s) 213
PflFI GACNNNGTC 2 cut(s) 85, 184
PkrI GCNGC 3 cut(s) 120, 174, 245
PspFI CCCAGC 1 cut(s) 247
PspN4I GGNNCC 1 cut(s) 235
PspPI GGNCC 1 cut(s) 234
PsyI GACNNNGTC 2 cut(s) 85, 184
SaqAI TTAA 1 cut(s) 435
SatI GCNGC 3 cut(s) 119, 173, 244
Sau96I GGNCC 1 cut(s) 234
ScrFI CCNGG 1 cut(s) 263
SetI ASST 8 cut(s) 18, 89, 108, 144, 306, 331, 354, 400
SinI GGWCC 1 cut(s) 234
SsiI CCGC 9 cut(s) 41, 118, 121, 164, 172, 175, 244, 321, 376
SspMI CTAG 1 cut(s) 395
StyD4I CCNGG 1 cut(s) 261
TaaI ACNGT 1 cut(s) 279
TaiI ACGT 1 cut(s) 89
TauI GCSGC 3 cut(s) 121, 175, 246
TfiI GAWTC 1 cut(s) 213
Tru1I TTAA 1 cut(s) 435
Tru9I TTAA 1 cut(s) 435
TspDTI ATGAA 1 cut(s) 149
Tth111I GACNNNGTC 2 cut(s) 85, 184
VpaK11BI GGWCC 1 cut(s) 234
XspI CTAG 1 cut(s) 395
ZraI GACGTC 1 cut(s) 87
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.