Rroxscaffold_1G00012330

UPF0503 protein At3g09070, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0010196
Physical Location & Seq
Reverse (-)
15210294 .. 15210836
543 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00012330.1

Sequence Viewer

Length: 543 bp
ATGGAGTCGAGTTCGTCGAATTCAAAGTCTGTGGAAGAGGATGGTGTGGTGGGGGGTGGTGGTGTTGGCAGCATTGAAGGTGTTTGTGGAAATGTAGCTGGTGTTTTGTCGAATGGGGTTGATCAGAAGCCGCAGAAATGGCACAAGAGGTGGAAGTTCTGGGGTTTGATGCAGAGGAAAAGCCAGGCGAAACATGGTGGTGGTGATCAAGAAGAGTCTTGGAAAAAGCTGAGGAGGGTTGCTAATGGAGAAGCCAATAATGCCTCTGTGAGCCAGAAGCTGATTCGAAGCTACAGTGTCAGCTGCAGAAACTCCTGCAAAATGGTGGGTTTGTTTAGTAATGTGAGCACTGTAGAGAGCAAAGGCAATGGCTTGAAGACGTTGCATAGGAACCGGAGCGCACGTTACTCACCGAGTAATCTTGATCACGGCCTCTTAAGGTTTTACTTGACACCATTGAGGAGTTATAGGAGGAGTGAATCGTGGAAGAGTAGGCTGCCAAACAACAACTCCCCGCAGACCTTGGCTAAGAATGTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF740

Protein Analysis

180

Amino Acids

19.87

Weight (kDa)

10.23

Isoelectric Point (pI)

66.16

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF740 PF05340 5 - 158 3.8e-21 Protein of unknown function (DUF740)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 2 cut(s) 131, 515
AcsI RAATTY 1 cut(s) 19
AflII CTTAAG 1 cut(s) 436
AgsI TTSAA 3 cut(s) 24, 77, 376
AjnI CCWGG 1 cut(s) 183
AluBI AGCT 5 cut(s) 98, 229, 280, 291, 303
AluI AGCT 5 cut(s) 98, 229, 280, 291, 303
Alw21I GWGCWC 1 cut(s) 350
AlwNI CAGNNNCTG 1 cut(s) 280
AoxI GGCC 1 cut(s) 430
ApeKI GCWGC 3 cut(s) 69, 303, 496
ApoI RAATTY 1 cut(s) 19
ArsI GACNNNNNNTTYG 2 cut(s) 11, 43
AspLEI GCGC 1 cut(s) 401
AsuHPI GGTGA 2 cut(s) 215, 402
AsuII TTCGAA 1 cut(s) 286
BbsI GAAGAC 1 cut(s) 383
Bbv12I GWGCWC 1 cut(s) 350
BbvCI CCTCAGC 1 cut(s) 230
BbvI GCAGC 3 cut(s) 81, 290, 483
BccI CCATC 1 cut(s) 35
BceAI ACGGC 1 cut(s) 445
BciT130I CCWGG 1 cut(s) 185
BclI TGATCA 3 cut(s) 121, 205, 424
BfmI CTRYAG 3 cut(s) 292, 304, 351
BfrI CTTAAG 1 cut(s) 436
BisI GCNGC 4 cut(s) 70, 131, 304, 497
BlsI GCNGC 4 cut(s) 71, 132, 305, 498
Bme1390I CCNGG 1 cut(s) 185
BmiI GGNNCC 1 cut(s) 392
BmrFI CCNGG 1 cut(s) 185
BmsI GCATC 1 cut(s) 159
BpiI GAAGAC 1 cut(s) 383
Bpu10I CCTNAGC 1 cut(s) 230
Bpu14I TTCGAA 1 cut(s) 286
BsaJI CCNNGG 1 cut(s) 522
BsaWI WCCGGW 1 cut(s) 393
BsaXI ACNNNNNCTCC 4 cut(s) 388, 418, 494, 524
Bse3DI GCAATG 1 cut(s) 373
BseBI CCWGG 1 cut(s) 185
BseDI CCNNGG 1 cut(s) 522
BseGI GGATG 1 cut(s) 46
BseMI GCAATG 1 cut(s) 373
BseMII CTCAG 1 cut(s) 221
BseRI GAGGAG 3 cut(s) 247, 475, 487
BseXI GCAGC 3 cut(s) 81, 290, 483
BshFI GGCC 1 cut(s) 432
BsiHKAI GWGCWC 1 cut(s) 350
BsiSI CCGG 1 cut(s) 394
BsnI GGCC 1 cut(s) 432
Bsp119I TTCGAA 1 cut(s) 286
Bsp1286I GDGCHC 1 cut(s) 350
Bsp143I GATC 3 cut(s) 121, 205, 424
BspACI CCGC 2 cut(s) 131, 515
BspANI GGCC 1 cut(s) 432
BspCNI CTCAG 1 cut(s) 222
BspLI GGNNCC 1 cut(s) 392
BspMAI CTGCAG 1 cut(s) 308
BspT104I TTCGAA 1 cut(s) 286
BspTI CTTAAG 1 cut(s) 436
BsrDI GCAATG 1 cut(s) 373
BssECI CCNNGG 1 cut(s) 522
BssMI GATC 3 cut(s) 121, 205, 424
BssT1I CCWWGG 1 cut(s) 522
Bst2UI CCWGG 1 cut(s) 185
Bst4CI ACNGT 2 cut(s) 296, 352
Bst6I CTCTTC 3 cut(s) 30, 207, 482
BstAFI CTTAAG 1 cut(s) 436
BstBI TTCGAA 1 cut(s) 286
BstDEI CTNAG 2 cut(s) 230, 528
BstF5I GGATG 1 cut(s) 46
BstHHI GCGC 1 cut(s) 401
BstKTI GATC 3 cut(s) 124, 208, 427
BstMBI GATC 3 cut(s) 121, 205, 424
BstMWI GCNNNNNNNGC 2 cut(s) 139, 260
BstNI CCWGG 1 cut(s) 185
BstSCI CCNGG 1 cut(s) 183
BstSFI CTRYAG 3 cut(s) 292, 304, 351
BstV1I GCAGC 3 cut(s) 81, 290, 483
BstV2I GAAGAC 1 cut(s) 383
BsuRI GGCC 1 cut(s) 432
BtsCI GGATG 1 cut(s) 46
BtsIMutI CAGTG 2 cut(s) 301, 348
CaiI CAGNNNCTG 1 cut(s) 280
CfoI GCGC 1 cut(s) 401
CspCI CAANNNNNGTGG 2 cut(s) 12, 47
CviAII CATG 1 cut(s) 194
DdeI CTNAG 2 cut(s) 230, 528
DpnI GATC 3 cut(s) 123, 207, 426
DpnII GATC 3 cut(s) 121, 205, 424
Eam1104I CTCTTC 3 cut(s) 30, 207, 482
EarI CTCTTC 3 cut(s) 30, 207, 482
Eco130I CCWWGG 1 cut(s) 522
EcoRI GAATTC 1 cut(s) 19
EcoRII CCWGG 1 cut(s) 183
EcoT14I CCWWGG 1 cut(s) 522
ErhI CCWWGG 1 cut(s) 522
FaeI CATG 1 cut(s) 197
FaiI YATR 3 cut(s) 195, 387, 468
FatI CATG 1 cut(s) 193
FauI CCCGC 1 cut(s) 522
FbaI TGATCA 3 cut(s) 121, 205, 424
Fnu4HI GCNGC 4 cut(s) 70, 131, 304, 497
FokI GGATG 1 cut(s) 53
Fsp4HI GCNGC 4 cut(s) 70, 131, 304, 497
GlaI GCGC 1 cut(s) 400
GluI GCNGC 4 cut(s) 70, 131, 304, 497
HaeIII GGCC 1 cut(s) 432
HapII CCGG 1 cut(s) 394
HhaI GCGC 1 cut(s) 401
Hin1II CATG 1 cut(s) 197
Hin6I GCGC 1 cut(s) 399
HinP1I GCGC 1 cut(s) 399
HinfI GANTC 4 cut(s) 5, 215, 283, 479
HpaII CCGG 1 cut(s) 394
HphI GGTGA 2 cut(s) 215, 402
Hpy188I TCNGA 1 cut(s) 126
Hpy188III TCNNGA 2 cut(s) 209, 422
Hpy99I CGWCG 1 cut(s) 19
HpyAV CCTTC 1 cut(s) 71
HpyCH4III ACNGT 2 cut(s) 296, 352
HpyCH4IV ACGT 2 cut(s) 380, 403
HpyCH4V TGCA 4 cut(s) 172, 306, 318, 385
HpyF10VI GCNNNNNNNGC 2 cut(s) 139, 260
HpyF3I CTNAG 2 cut(s) 230, 528
HpySE526I ACGT 2 cut(s) 380, 403
Hsp92II CATG 1 cut(s) 197
HspAI GCGC 1 cut(s) 399
Ksp22I TGATCA 3 cut(s) 121, 205, 424
Kzo9I GATC 3 cut(s) 121, 205, 424
LmnI GCTCC 1 cut(s) 396
LpnPI CCDG 7 cut(s) 84, 145, 170, 197, 287, 328, 407
Lsp1109I GCAGC 3 cut(s) 81, 290, 483
LweI GCATC 1 cut(s) 159
MaeII ACGT 2 cut(s) 380, 403
MaeIII GTNAC 1 cut(s) 404
MalI GATC 3 cut(s) 123, 207, 426
MboI GATC 3 cut(s) 121, 205, 424
MboII GAAGA 4 cut(s) 47, 224, 388, 499
MhlI GDGCHC 1 cut(s) 350
MluCI AATT 1 cut(s) 19
MlyI GAGTC 2 cut(s) 14, 224
MnlI CCTC 9 cut(s) 31, 141, 168, 225, 228, 274, 443, 453, 465
MseI TTAA 1 cut(s) 437
MslI CAYNNNNRTG 1 cut(s) 198
MspA1I CMGCKG 1 cut(s) 303
MspCI CTTAAG 1 cut(s) 436
MspI CCGG 1 cut(s) 394
MspR9I CCNGG 1 cut(s) 185
MvaI CCWGG 1 cut(s) 185
MwoI GCNNNNNNNGC 2 cut(s) 139, 260
NdeII GATC 3 cut(s) 121, 205, 424
NlaIII CATG 1 cut(s) 197
NlaIV GGNNCC 1 cut(s) 392
NspV TTCGAA 1 cut(s) 286
PcsI WCGNNNNNNNCGW 1 cut(s) 14
PfeI GAWTC 2 cut(s) 283, 479
PkrI GCNGC 4 cut(s) 71, 132, 305, 498
PleI GAGTC 2 cut(s) 13, 223
PpsI GAGTC 2 cut(s) 13, 223
Psp6I CCWGG 1 cut(s) 183
PspGI CCWGG 1 cut(s) 183
PspN4I GGNNCC 1 cut(s) 392
PstI CTGCAG 1 cut(s) 308
PstNI CAGNNNCTG 1 cut(s) 280
PvuII CAGCTG 1 cut(s) 303
RseI CAYNNNNRTG 1 cut(s) 198
SaqAI TTAA 1 cut(s) 437
SatI GCNGC 4 cut(s) 70, 131, 304, 497
Sau3AI GATC 3 cut(s) 121, 205, 424
SchI GAGTC 2 cut(s) 14, 224
ScrFI CCNGG 1 cut(s) 185
SduI GDGCHC 1 cut(s) 350
SfaNI GCATC 1 cut(s) 159
SfcI CTRYAG 3 cut(s) 292, 304, 351
SfuI TTCGAA 1 cut(s) 286
SmiMI CAYNNNNRTG 1 cut(s) 198
SmlI CTYRAG 1 cut(s) 436
SmoI CTYRAG 1 cut(s) 436
Sse9I AATT 1 cut(s) 19
SsiI CCGC 2 cut(s) 131, 515
StyD4I CCNGG 1 cut(s) 183
StyI CCWWGG 1 cut(s) 522
TaaI ACNGT 2 cut(s) 296, 352
TaiI ACGT 2 cut(s) 383, 406
TaqI TCGA 4 cut(s) 8, 17, 110, 286
TasI AATT 1 cut(s) 19
TauI GCSGC 1 cut(s) 133
TfiI GAWTC 2 cut(s) 283, 479
Tru1I TTAA 1 cut(s) 437
Tru9I TTAA 1 cut(s) 437
TscAI CASTG 2 cut(s) 301, 355
TseI GCWGC 3 cut(s) 69, 303, 496
TspRI CASTG 2 cut(s) 301, 355
Vha464I CTTAAG 1 cut(s) 436
XapI RAATTY 1 cut(s) 19
XcmI CCANNNNNNNNNTGG 1 cut(s) 191
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.