Basic Information

Type: gene

Biological Identity

Species

pyrus_communis

Chromosome

Chr3

Orthogroup ID

OG0010196

Physical Location & Seq

Genomic Coordinates Reverse (-)

4843002 .. 4844528

Gene Structure 1527 bp

Loading structure...

UTR

Exon/CDS

Intron

Transcript / Protein ID

pycom03g06090.1

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1527 bp

ATGAAGGCGCAAACTCACCGCCTCTCCACCTGTCACCGCCACCCTACCAAACCCATAACCGGTTTCTGCGCCCCATGCCTCACCGAACGCCTCGCCGGCCTCGACTCCGCCGCCGCTCACCAAGAACCCTCGACCCAGAACCCCCATTCTGGGTCTGAGCTCCGGAGAAGCAAGTCGTGTTCCGGAACGAGACCCGAATCGTCGTCGGCCAGCGCCCCGGATCCGACCCGGCGCAAGTCGTGCAATGCCAGGGCCCGGAACACTCTGTCCGAGCTCTTTAACATCGACGACGGCCGCAAAGGAGTGCCGAGAAAGTTCCATGTCGAATTGAGAGATGGGGATAAGGACGATGGTGGAGACGAAGCTAGGGTTTTGGATTTGGATACGGGTGCAGATTCGGAGTTCAAGACGATGAAAGAGTTGATAGATCTCGAGTGGGAGAAGAAGAAGGGCGAGGGTAGGGATTCGAAGGAAATTGCAGGGACATTTTGGGGAACGACGTCGGTTTTGGGCAAAAAGGTGAAGCAATGGCGGCGGAAGCAGAAGACGAAGAAGGCTGAGAACGAGGGTTTCGTGGCGGAGAAGAGGAGGGAGACCCAGTCCGAAGTGGGAGAGTACGCATTGGGGAGAAAATCGTGCGATACGGATCCCGATCCGGGTCGGATGTCATTGGACGAGCCTCGGGCTTCTTGGGACGGGTATTTGGGTCCGAGAGCCCCTCACCCGAGGCTGACCCCGATGGTTTCGGTTGTGGAAGAGAGCTGGGAGAGGAGTCCAGGCGGCTCAGCTCAGACCAAAGACTATTACAATTCGCAGCGGCGGCGGAGCTTCGAACGGCCGAGTTTCGATCGGTCCGCAACGGCGGAGGTTGATGAGCTGAAGTTGGGATCAAATGCTAGAGTCTCCCCTGCCACTTCAGAGTTGTTCTATGGTGCCAAGCTGCTGATTACAGAGAAAGAATTGATGAGTTCGATGGGTTCGAATTCGAAGTTGAAATCTGCTGAAGATGATGAGCGTGTGGAGGAGGGCAGCGGCGTTGGGATTGTCTCTGGACATGTTTGCAAAAATGCGGCTTCGGAATCAACCAATGGGGTTGATGTTGATCATCAAAAGTCGAATAGGAGGCACAAGAAGTGGAGCTTTTGGGGTTTGATGCAGAGGAGAAGCGAGAAGAAATGCGTAGACGAAGAATCCGCGGATTCTTGGCAGAAGCTAAGGAGGGTGGCCAACGGAGAAGAAGCCAACGCCTCCGTGAGTCAGAAGCTGATTCGAAGTTACAGTGTCAGCTGCCGGAACCAGTGCAGAATGGTTGGCTTGTTAAGCAATGTGAATGGTGTTGGTGCGGAAACTAAAAGCAATGAAGTGAAGACTCTGCAGAGGAACAAGAGTGCTCGGTATTCGCCGAGCAACCTCGATCACGGGTTGCTGCGGTTCTACTTGACGCCGTTGAGGAGCTATCGGAGGAGTGAATCGGGAAAGAGCAGGCCAGGCAGCAACTCACAATATCTGTCCAAGAATGTCTTGTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

No ontology terms assigned.

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

DUF740

Protein Analysis

509

Amino Acids

56.66

Weight (kDa)

9.38

Isoelectric Point (pI)

53.11

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
DUF740	PF05340	4 - 45	6.8e-10	Protein of unknown function (DUF740)
DUF740	PF05340	51 - 107	9e-09	Protein of unknown function (DUF740)
DUF740	PF05340	130 - 488	7e-74	Protein of unknown function (DUF740)

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0010196)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT3G46990 AT5G58930
fragaria_vesca	FvH4_3g38100
malus_domestica	MD03G1077100.v1.1 MD11G1081400.v1.1
prunus_persica	Prupe.6G061700_v2.0.a1
pyrus_communis	pycom03g06090 pycom05g14380 pycom15g27530
rosa_chinensis	RchiOBHm_Chr5g0068451
rosa_laevigata	RLG00000035976
rosa_multiflora	Rmu_sc0005087.1_g000009
rosa_roxburghii	Rroxscaffold_1G00012330 Rroxscaffold_1G00012340 Rroxscaffold_1G00013070
rosa_rugosa	Rorug05G0390100
rosa_samantha	Rh5AG448700 Rh5BG466800 Rh5CG488400 Rh5DG480200
rosa_wichuraiana	Rw5G041880

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AatII	GACGTC	1 cut(s)	503
AccB1I	GGYRCC	1 cut(s)	932
AccBSI	CCGCTC	1 cut(s)	116
AccI	GTMKAC	1 cut(s)	1182
AccII	CGCG	1 cut(s)	1196
AccIII	TCCGGA	2 cut(s)	162, 182
AclWI	GGATC	6 cut(s)	215, 228, 641, 647, 654, 895
AcoI	YGGCCR	4 cut(s)	207, 292, 836, 1224
AcsI	RAATTY	1 cut(s)	982
AcuI	CTGAAG	3 cut(s)	899, 900, 1023
AcyI	GRCGYC	2 cut(s)	500, 1442
AfaI	GTAC	1 cut(s)	617
AfiI	CCNNNNNNNGG	5 cut(s)	59, 149, 150, 255, 656
AflIII	ACRYGT	1 cut(s)	1054
AgeI	ACCGGT	1 cut(s)	59
AgsI	TTSAA	2 cut(s)	406, 994
AjnI	CCWGG	3 cut(s)	248, 775, 1486
AloI	GAACNNNNNNTCC	2 cut(s)	251, 283
Alw21I	GWGCWC	3 cut(s)	162, 276, 1393
Alw26I	GTCTC	5 cut(s)	184, 351, 587, 907, 1051
AlwI	GGATC	6 cut(s)	215, 228, 641, 647, 654, 895
AlwNI	CAGNNNCTG	1 cut(s)	1264
Ama87I	CYCGRG	3 cut(s)	431, 681, 724
Aor13HI	TCCGGA	2 cut(s)	162, 182
AoxI	GGCC	7 cut(s)	97, 207, 252, 292, 836, 1224, 1484
ApaI	GGGCCC	1 cut(s)	256
ApeKI	GCWGC	6 cut(s)	814, 940, 1029, 1287, 1426, 1491
ApoI	RAATTY	1 cut(s)	982
ArsI	GACNNNNNNTTYG	2 cut(s)	490, 522
AsiGI	ACCGGT	1 cut(s)	59
AspLEI	GCGC	4 cut(s)	10, 71, 215, 234
AspS9I	GGNCC	4 cut(s)	252, 253, 707, 852
AsuC2I	CCSGG	4 cut(s)	218, 229, 256, 657
AsuHPI	GGTGA	6 cut(s)	8, 26, 73, 110, 532, 713
AsuII	TTCGAA	5 cut(s)	467, 831, 980, 986, 1270
AvaI	CYCGRG	3 cut(s)	431, 681, 724
AvaII	GGWCC	2 cut(s)	707, 852
BaeGI	GKGCMC	1 cut(s)	256
BalI	TGGCCA	1 cut(s)	1226
BamHI	GGATCC	2 cut(s)	220, 646
BanI	GGYRCC	1 cut(s)	932
BanII	GRGCYC	4 cut(s)	162, 256, 276, 718
BbsI	GAAGAC	2 cut(s)	551, 1373
Bbv12I	GWGCWC	3 cut(s)	162, 276, 1393
BbvI	GCAGC	6 cut(s)	826, 927, 1041, 1274, 1413, 1503
BccI	CCATC	4 cut(s)	329, 344, 733, 967
BceAI	ACGGC	4 cut(s)	307, 851, 876, 1429
BciT130I	CCWGG	3 cut(s)	250, 777, 1488
BciVI	GTATCC	1 cut(s)	376
BclI	TGATCA	1 cut(s)	1102
BcnI	CCSGG	4 cut(s)	218, 229, 256, 657
BcoDI	GTCTC	5 cut(s)	184, 351, 587, 907, 1051
BfaI	CTAG	2 cut(s)	366, 897
BfmI	CTRYAG	1 cut(s)	1373
BfoI	RGCGCY	1 cut(s)	216
BfuI	GTATCC	1 cut(s)	376
BglI	GCCNNNNNGGC	1 cut(s)	96
BglII	AGATCT	1 cut(s)	427
BlpI	GCTNAGC	1 cut(s)	784
Bme1390I	CCNGG	7 cut(s)	218, 229, 250, 256, 657, 777, 1488
Bme18I	GGWCC	2 cut(s)	707, 852
BmeT110I	CYCGRG	3 cut(s)	431, 681, 724
BmgT120I	GGNCC	4 cut(s)	252, 253, 707, 852
BmiI	GGNNCC	6 cut(s)	222, 254, 648, 708, 934, 1295
BmrFI	CCNGG	7 cut(s)	218, 229, 250, 256, 657, 777, 1488
BmrI	ACTGGG	1 cut(s)	592
BmsI	GCATC	1 cut(s)	1143
BmuI	ACTGGG	1 cut(s)	592
BpiI	GAAGAC	2 cut(s)	551, 1373
BplI	GAGNNNNNCTC	2 cut(s)	703, 735
Bpu10I	CCTNAGC	1 cut(s)	1214
Bpu1102I	GCTNAGC	1 cut(s)	784
Bpu14I	TTCGAA	5 cut(s)	467, 831, 980, 986, 1270
BpuMI	CCSGG	4 cut(s)	218, 229, 256, 657
BsaBI	GATNNNNATC	3 cut(s)	645, 651, 1101
BsaHI	GRCGYC	2 cut(s)	500, 1442
BsaI	GGTCTC	2 cut(s)	184, 587
BsaJI	CCNNGG	5 cut(s)	216, 249, 680, 725, 1194
BsaWI	WCCGGW	3 cut(s)	59, 162, 182
BsaXI	ACNNNNNCTCC	4 cut(s)	8, 38, 584, 614
Bsc4I	CCNNNNNNNGG	5 cut(s)	59, 149, 150, 255, 656
Bse118I	RCCGGY	2 cut(s)	59, 95
Bse1I	ACTGG	2 cut(s)	598, 1297
Bse3DI	GCAATG	4 cut(s)	250, 533, 1330, 1363
Bse8I	GATNNNNATC	3 cut(s)	645, 651, 1101
BseAI	TCCGGA	2 cut(s)	162, 182
BseBI	CCWGG	3 cut(s)	250, 777, 1488
BseDI	CCNNGG	5 cut(s)	216, 249, 680, 725, 1194
BseGI	GGATG	1 cut(s)	669
BseJI	GATNNNNATC	3 cut(s)	645, 651, 1101
BseLI	CCNNNNNNNGG	5 cut(s)	59, 149, 150, 255, 656
BseMI	GCAATG	4 cut(s)	250, 533, 1330, 1363
BseMII	CTCAG	4 cut(s)	147, 549, 798, 803
BseNI	ACTGG	2 cut(s)	598, 1297
BseRI	GAGGAG	6 cut(s)	601, 784, 1037, 1174, 1465, 1477
BseSI	GKGCMC	1 cut(s)	256
BseX3I	CGGCCG	2 cut(s)	292, 836
BseXI	GCAGC	6 cut(s)	826, 927, 1041, 1274, 1413, 1503
BseYI	CCCAGC	1 cut(s)	762
BsgI	GTGCAG	2 cut(s)	411, 1321
Bsh1236I	CGCG	1 cut(s)	1196
Bsh1285I	CGRYCG	3 cut(s)	295, 839, 850
BshFI	GGCC	7 cut(s)	99, 209, 254, 294, 838, 1226, 1486
BshNI	GGYRCC	1 cut(s)	932
BshTI	ACCGGT	1 cut(s)	59
BsiEI	CGRYCG	3 cut(s)	295, 839, 850
BsiHKAI	GWGCWC	3 cut(s)	162, 276, 1393
BsiHKCI	CYCGRG	3 cut(s)	431, 681, 724
BsiSI	CCGG	9 cut(s)	60, 96, 163, 183, 218, 229, 256, 656, 1291
BslFI	GGGAC	2 cut(s)	496, 707
BslI	CCNNNNNNNGG	5 cut(s)	59, 149, 150, 255, 656
BsmAI	GTCTC	5 cut(s)	184, 351, 587, 907, 1051
BsmBI	CGTCTC	1 cut(s)	351
BsmFI	GGGAC	2 cut(s)	496, 707
BsnI	GGCC	7 cut(s)	99, 209, 254, 294, 838, 1226, 1486
Bso31I	GGTCTC	2 cut(s)	184, 587
BsoBI	CYCGRG	3 cut(s)	431, 681, 724
Bsp119I	TTCGAA	5 cut(s)	467, 831, 980, 986, 1270
Bsp120I	GGGCCC	1 cut(s)	252
Bsp1286I	GDGCHC	5 cut(s)	162, 256, 276, 718, 1393
Bsp13I	TCCGGA	2 cut(s)	162, 182
Bsp143I	GATC	8 cut(s)	220, 427, 646, 652, 847, 887, 1102, 1414
Bsp1720I	GCTNAGC	1 cut(s)	784
BspANI	GGCC	7 cut(s)	99, 209, 254, 294, 838, 1226, 1486
BspCNI	CTCAG	4 cut(s)	148, 550, 797, 802
BspEI	TCCGGA	2 cut(s)	162, 182
BspFNI	CGCG	1 cut(s)	1196
BspLI	GGNNCC	6 cut(s)	222, 254, 648, 708, 934, 1295
BspMAI	CTGCAG	1 cut(s)	1377
BspPI	GGATC	6 cut(s)	215, 228, 641, 647, 654, 895
BspT104I	TTCGAA	5 cut(s)	467, 831, 980, 986, 1270
BspT107I	GGYRCC	1 cut(s)	932
BspTNI	GGTCTC	2 cut(s)	184, 587
BsrBI	CCGCTC	1 cut(s)	116
BsrDI	GCAATG	4 cut(s)	250, 533, 1330, 1363
BsrFI	RCCGGY	2 cut(s)	59, 95
BsrI	ACTGG	2 cut(s)	598, 1297
BssAI	RCCGGY	2 cut(s)	59, 95
BssECI	CCNNGG	5 cut(s)	216, 249, 680, 725, 1194
BssMI	GATC	8 cut(s)	220, 427, 646, 652, 847, 887, 1102, 1414
BssNI	GRCGYC	2 cut(s)	500, 1442
Bst2UI	CCWGG	3 cut(s)	250, 777, 1488
Bst4CI	ACNGT	1 cut(s)	1280
Bst6I	CTCTTC	2 cut(s)	578, 750
BstACI	GRCGYC	2 cut(s)	500, 1442
BstAPI	GCANNNNNTGC	1 cut(s)	240
BstBI	TTCGAA	5 cut(s)	467, 831, 980, 986, 1270
BstC8I	GCNNGC	3 cut(s)	97, 211, 1484
BstDEI	CTNAG	5 cut(s)	156, 558, 784, 789, 1214
BstDSI	CCRYGG	1 cut(s)	1194
BstF5I	GGATG	1 cut(s)	669
BstFNI	CGCG	1 cut(s)	1196
BstH2I	RGCGCY	1 cut(s)	216
BstHHI	GCGC	4 cut(s)	10, 71, 215, 234
BstKTI	GATC	8 cut(s)	223, 430, 649, 655, 850, 890, 1105, 1417
BstMAI	GTCTC	5 cut(s)	184, 351, 587, 907, 1051
BstMBI	GATC	8 cut(s)	220, 427, 646, 652, 847, 887, 1102, 1414
BstMCI	CGRYCG	3 cut(s)	295, 839, 850
BstMWI	GCNNNNNNNGC	8 cut(s)	75, 96, 240, 532, 538, 820, 1320, 1488
BstNI	CCWGG	3 cut(s)	250, 777, 1488
BstNSI	RCATGY	1 cut(s)	1058
BstSCI	CCNGG	7 cut(s)	216, 227, 248, 254, 655, 775, 1486
BstSFI	CTRYAG	1 cut(s)	1373
BstSLI	GKGCMC	1 cut(s)	256
BstUI	CGCG	1 cut(s)	1196
BstV1I	GCAGC	6 cut(s)	826, 927, 1041, 1274, 1413, 1503
BstV2I	GAAGAC	2 cut(s)	551, 1373
BstX2I	RGATCY	3 cut(s)	220, 427, 646
BstYI	RGATCY	3 cut(s)	220, 427, 646
BstZI	CGGCCG	2 cut(s)	292, 836
BsuI	GTATCC	1 cut(s)	376
BsuRI	GGCC	7 cut(s)	99, 209, 254, 294, 838, 1226, 1486
BtgI	CCRYGG	1 cut(s)	1194
BtsCI	GGATG	1 cut(s)	669
BtsIMutI	CAGTG	2 cut(s)	1285, 1304
Cac8I	GCNNGC	3 cut(s)	97, 211, 1484
CaiI	CAGNNNCTG	1 cut(s)	1264
CfoI	GCGC	4 cut(s)	10, 71, 215, 234
Cfr10I	RCCGGY	2 cut(s)	59, 95
Cfr13I	GGNCC	4 cut(s)	252, 253, 707, 852
Cfr42I	CCGCGG	1 cut(s)	1197
CpoI	CGGWCCG	1 cut(s)	852
CseI	GACGC	1 cut(s)	1450
Csp6I	GTAC	1 cut(s)	616
CspAI	ACCGGT	1 cut(s)	59
CspI	CGGWCCG	1 cut(s)	852
CviAII	CATG	3 cut(s)	75, 320, 1055
CviQI	GTAC	1 cut(s)	616
DdeI	CTNAG	5 cut(s)	156, 558, 784, 789, 1214
DpnI	GATC	8 cut(s)	222, 429, 648, 654, 849, 889, 1104, 1416
DpnII	GATC	8 cut(s)	220, 427, 646, 652, 847, 887, 1102, 1414
EaeI	YGGCCR	4 cut(s)	207, 292, 836, 1224
EagI	CGGCCG	2 cut(s)	292, 836
Eam1104I	CTCTTC	2 cut(s)	578, 750
EarI	CTCTTC	2 cut(s)	578, 750
EciI	GGCGGA	5 cut(s)	97, 550, 593, 838, 878
Ecl136II	GAGCTC	2 cut(s)	160, 274
EclXI	CGGCCG	2 cut(s)	292, 836
Eco24I	GRGCYC	4 cut(s)	162, 256, 276, 718
Eco31I	GGTCTC	2 cut(s)	184, 587
Eco47I	GGWCC	2 cut(s)	707, 852
Eco52I	CGGCCG	2 cut(s)	292, 836
Eco53kI	GAGCTC	2 cut(s)	160, 274
Eco57I	CTGAAG	3 cut(s)	899, 900, 1023
Eco88I	CYCGRG	3 cut(s)	431, 681, 724
EcoICRI	GAGCTC	2 cut(s)	160, 274
EcoO109I	RGGNCCY	1 cut(s)	252
EcoRI	GAATTC	1 cut(s)	982
EcoRII	CCWGG	3 cut(s)	248, 775, 1486
EcoT38I	GRGCYC	4 cut(s)	162, 256, 276, 718
Esp3I	CGTCTC	1 cut(s)	351
FaeI	CATG	3 cut(s)	78, 323, 1058
FaiI	YATR	5 cut(s)	56, 76, 321, 930, 1056
FalI	AAGNNNNNCTT	3 cut(s)	1124, 1156, 1505
FaqI	GGGAC	2 cut(s)	496, 707
FatI	CATG	3 cut(s)	74, 319, 1054
FbaI	TGATCA	1 cut(s)	1102
FblI	GTMKAC	1 cut(s)	1182
FokI	GGATG	1 cut(s)	676
FriOI	GRGCYC	4 cut(s)	162, 256, 276, 718
FspBI	CTAG	2 cut(s)	366, 897
GlaI	GCGC	4 cut(s)	9, 70, 214, 233
GsaI	CCCAGC	1 cut(s)	766
HaeII	RGCGCY	1 cut(s)	216
HaeIII	GGCC	7 cut(s)	99, 209, 254, 294, 838, 1226, 1486
HapII	CCGG	9 cut(s)	60, 96, 163, 183, 218, 229, 256, 656, 1291
HgaI	GACGC	1 cut(s)	1450
HhaI	GCGC	4 cut(s)	10, 71, 215, 234
Hin1I	GRCGYC	2 cut(s)	500, 1442
Hin1II	CATG	3 cut(s)	78, 323, 1058
Hin6I	GCGC	4 cut(s)	8, 69, 213, 232
HinP1I	GCGC	4 cut(s)	8, 69, 213, 232
HpaII	CCGG	9 cut(s)	60, 96, 163, 183, 218, 229, 256, 656, 1291
HphI	GGTGA	6 cut(s)	8, 26, 73, 110, 532, 713
Hpy166II	GTNNAC	1 cut(s)	1183
Hpy188III	TCNNGA	7 cut(s)	163, 183, 406, 431, 650, 1050, 1473
Hpy8I	GTNNAC	1 cut(s)	1183
Hpy99I	CGWCG	6 cut(s)	205, 208, 290, 293, 502, 505
HpyAV	CCTTC	3 cut(s)	442, 463, 547
HpyCH4III	ACNGT	1 cut(s)	1280
HpyCH4IV	ACGT	1 cut(s)	500
HpyCH4V	TGCA	7 cut(s)	243, 392, 479, 1062, 1156, 1302, 1375
HpyF10VI	GCNNNNNNNGC	8 cut(s)	75, 96, 240, 532, 538, 820, 1320, 1488
HpyF3I	CTNAG	5 cut(s)	156, 558, 784, 789, 1214
HpySE526I	ACGT	1 cut(s)	500
Hsp92I	GRCGYC	2 cut(s)	500, 1442
Hsp92II	CATG	3 cut(s)	78, 323, 1058
HspAI	GCGC	4 cut(s)	8, 69, 213, 232
Kpn2I	TCCGGA	2 cut(s)	162, 182
KroI	GCCGGC	1 cut(s)	95
KroNI	GCCGGC	1 cut(s)	97
Ksp22I	TGATCA	1 cut(s)	1102
KspI	CCGCGG	1 cut(s)	1197
Kzo9I	GATC	8 cut(s)	220, 427, 646, 652, 847, 887, 1102, 1414
LmnI	GCTCC	4 cut(s)	165, 825, 1137, 1452
Lsp1109I	GCAGC	6 cut(s)	826, 927, 1041, 1274, 1413, 1503
LweI	GCATC	1 cut(s)	1143
MaeI	CTAG	2 cut(s)	366, 897
MaeII	ACGT	1 cut(s)	500
MaeIII	GTNAC	2 cut(s)	32, 1274
MalI	GATC	8 cut(s)	222, 429, 648, 654, 849, 889, 1104, 1416
MbiI	CCGCTC	1 cut(s)	116
MboI	GATC	8 cut(s)	220, 427, 646, 652, 847, 887, 1102, 1414
MflI	RGATCY	3 cut(s)	220, 427, 646
MhlI	GDGCHC	5 cut(s)	162, 256, 276, 718, 1393
MlsI	TGGCCA	1 cut(s)	1226
MluCI	AATT	5 cut(s)	326, 474, 808, 959, 982
MluNI	TGGCCA	1 cut(s)	1226
MlyI	GAGTC	5 cut(s)	98, 781, 909, 1264, 1363
MmeI	TCCRAC	2 cut(s)	248, 641
Mox20I	TGGCCA	1 cut(s)	1226
MroI	TCCGGA	2 cut(s)	162, 182
MroNI	GCCGGC	1 cut(s)	95
MscI	TGGCCA	1 cut(s)	1226
MseI	TTAA	2 cut(s)	279, 1319
Msp20I	TGGCCA	1 cut(s)	1226
MspA1I	CMGCKG	4 cut(s)	817, 1032, 1196, 1287
MspI	CCGG	9 cut(s)	60, 96, 163, 183, 218, 229, 256, 656, 1291
MspR9I	CCNGG	7 cut(s)	218, 229, 250, 256, 657, 777, 1488
MvaI	CCWGG	3 cut(s)	250, 777, 1488
MvnI	CGCG	1 cut(s)	1196
MwoI	GCNNNNNNNGC	8 cut(s)	75, 96, 240, 532, 538, 820, 1320, 1488
NaeI	GCCGGC	1 cut(s)	97
NciI	CCSGG	4 cut(s)	218, 229, 256, 657
NdeII	GATC	8 cut(s)	220, 427, 646, 652, 847, 887, 1102, 1414
NgoMIV	GCCGGC	1 cut(s)	95
NlaIII	CATG	3 cut(s)	78, 323, 1058
NlaIV	GGNNCC	6 cut(s)	222, 254, 648, 708, 934, 1295
NmeAIII	GCCGAG	3 cut(s)	333, 864, 1428
NmuCI	GTSAC	1 cut(s)	32
NspI	RCATGY	1 cut(s)	1058
NspV	TTCGAA	5 cut(s)	467, 831, 980, 986, 1270
PaeR7I	CTCGAG	1 cut(s)	431
PciI	ACATGT	1 cut(s)	1054
PcsI	WCGNNNNNNNCGW	3 cut(s)	99, 570, 977
PdiI	GCCGGC	1 cut(s)	97
PfeI	GAWTC	8 cut(s)	197, 395, 464, 1079, 1190, 1199, 1267, 1469
PflFI	GACNNNGTC	1 cut(s)	598
PinAI	ACCGGT	1 cut(s)	59
Ple19I	CGATCG	1 cut(s)	850
PleI	GAGTC	5 cut(s)	98, 780, 908, 1263, 1363
PpsI	GAGTC	5 cut(s)	98, 780, 908, 1263, 1363
PscI	ACATGT	1 cut(s)	1054
Psp124BI	GAGCTC	2 cut(s)	162, 276
Psp6I	CCWGG	3 cut(s)	248, 775, 1486
PspFI	CCCAGC	1 cut(s)	762
PspGI	CCWGG	3 cut(s)	248, 775, 1486
PspN4I	GGNNCC	6 cut(s)	222, 254, 648, 708, 934, 1295
PspOMI	GGGCCC	1 cut(s)	252
PspPI	GGNCC	4 cut(s)	252, 253, 707, 852
PstI	CTGCAG	1 cut(s)	1377
PstNI	CAGNNNCTG	1 cut(s)	1264
PsuI	RGATCY	3 cut(s)	220, 427, 646
PsyI	GACNNNGTC	1 cut(s)	598
PvuI	CGATCG	1 cut(s)	850
PvuII	CAGCTG	1 cut(s)	1287
RsaI	GTAC	1 cut(s)	617
RsaNI	GTAC	1 cut(s)	616
Rsr2I	CGGWCCG	1 cut(s)	852
RsrII	CGGWCCG	1 cut(s)	852
SacI	GAGCTC	2 cut(s)	162, 276
SacII	CCGCGG	1 cut(s)	1197
SaqAI	TTAA	2 cut(s)	279, 1319
Sau3AI	GATC	8 cut(s)	220, 427, 646, 652, 847, 887, 1102, 1414
Sau96I	GGNCC	4 cut(s)	252, 253, 707, 852
SchI	GAGTC	5 cut(s)	98, 781, 909, 1264, 1363
ScrFI	CCNGG	7 cut(s)	218, 229, 250, 256, 657, 777, 1488
SduI	GDGCHC	5 cut(s)	162, 256, 276, 718, 1393
SfaNI	GCATC	1 cut(s)	1143
SfcI	CTRYAG	1 cut(s)	1373
Sfr274I	CTCGAG	1 cut(s)	431
Sfr303I	CCGCGG	1 cut(s)	1197
SfuI	TTCGAA	5 cut(s)	467, 831, 980, 986, 1270
SgrBI	CCGCGG	1 cut(s)	1197
SinI	GGWCC	2 cut(s)	707, 852
SlaI	CTCGAG	1 cut(s)	431
SmlI	CTYRAG	1 cut(s)	431
SmoI	CTYRAG	1 cut(s)	431
Sse9I	AATT	5 cut(s)	326, 474, 808, 959, 982
SspMI	CTAG	2 cut(s)	366, 897
SstI	GAGCTC	2 cut(s)	162, 276
StyD4I	CCNGG	7 cut(s)	216, 227, 248, 254, 655, 775, 1486
TaaI	ACNGT	1 cut(s)	1280
TaiI	ACGT	1 cut(s)	503
TaqII	GACCGA	1 cut(s)	840
TasI	AATT	5 cut(s)	326, 474, 808, 959, 982
TauI	GCSGC	9 cut(s)	113, 116, 297, 535, 783, 820, 823, 1035, 1073
TfiI	GAWTC	8 cut(s)	197, 395, 464, 1079, 1190, 1199, 1267, 1469
Tru1I	TTAA	2 cut(s)	279, 1319
Tru9I	TTAA	2 cut(s)	279, 1319
TscAI	CASTG	2 cut(s)	1285, 1304
TseFI	GTSAC	1 cut(s)	32
TseI	GCWGC	6 cut(s)	814, 940, 1029, 1287, 1426, 1491
Tsp45I	GTSAC	1 cut(s)	32
TspDTI	ATGAA	3 cut(s)	17, 428, 1374
TspGWI	ACGGA	3 cut(s)	659, 1240, 1245
TspRI	CASTG	2 cut(s)	1285, 1304
Tth111I	GACNNNGTC	1 cut(s)	598
VpaK11BI	GGWCC	2 cut(s)	707, 852
XapI	RAATTY	1 cut(s)	982
XceI	RCATGY	1 cut(s)	1058
XhoI	CTCGAG	1 cut(s)	431
XmiI	GTMKAC	1 cut(s)	1182
XspI	CTAG	2 cut(s)	366, 897
ZraI	GACGTC	1 cut(s)	501

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.