Rroxscaffold_1G00013070

UPF0503 protein At3g09070, chloroplastic-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000001
OG0010196
Physical Location & Seq
Reverse (-)
16130990 .. 16133172
2183 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_1G00013070.1

Sequence Viewer

Length: 636 bp
ATGCCGGCGGAGGTGGATGAGTTGAAGCTGGTGTCGAATGGCGCTACGGAGTTGTTCTATGGGGCAAAGCTGCTGATTACGGAGAAGGAATTGATGGAGTCGAGTTCGTCGAATTCAAAGTCTGTGGAAGAGGATGGTGTGGTGGGGGGTGGTGGTGTTGGCAGCATTGAAGGTGTTTGTGGAAATGTAGCTGGTGTTTTGTCGAATGGGGTTGATCAGAAGCCGCAGAAATGGCACAAGAGGTGGAAGTTCTGGGGTTTGATGCAGAGGAAAAGCCAGGCGAAACATGGTGGTGGTGATCAAGAAGAGTCTTGGAAAAAGCTGAGGAGGGTTGCTAATGGAGAAGCCAATAATGCCTCTGTGAGCCAGAAGCTGATTCGAAGCTACAGTGTCAGCTGCAGAAACTCCTGCAAAATGGTGGGTTTGTTTAGTAATGTGAGCACTGTAGAGAGCAAAGGCAATGGCTTGAAGACGTTGCATAGGAACCGGAGCGCACGTTACTCACCGAGTAATCTTGATCACGGCCTCTTAAGGTTTTACTTGACACCATTGAGGAGTTATAGGAGGAGTGAATCGTGGAAGAGTAGGCTGCCAAACAACAACTCCCCGCAGACCTTGGCTAAGAATGTGTTGTAA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
No pathways identified.
Pfam Domains
Protein Families
DUF740

Protein Analysis

211

Amino Acids

23.27

Weight (kDa)

9.83

Isoelectric Point (pI)

60.43

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
DUF740 PF05340 3 - 189 7.6e-26 Protein of unknown function (DUF740)
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 3 cut(s) 8, 224, 608
AcsI RAATTY 1 cut(s) 112
AflII CTTAAG 1 cut(s) 529
AgsI TTSAA 4 cut(s) 25, 117, 170, 469
AjnI CCWGG 1 cut(s) 276
AluBI AGCT 7 cut(s) 28, 70, 191, 322, 373, 384, 396
AluI AGCT 7 cut(s) 28, 70, 191, 322, 373, 384, 396
Alw21I GWGCWC 1 cut(s) 443
AlwNI CAGNNNCTG 1 cut(s) 373
AoxI GGCC 1 cut(s) 523
ApeKI GCWGC 4 cut(s) 70, 162, 396, 589
ApoI RAATTY 1 cut(s) 112
ArsI GACNNNNNNTTYG 2 cut(s) 104, 136
AspLEI GCGC 2 cut(s) 44, 494
AsuHPI GGTGA 2 cut(s) 308, 495
AsuII TTCGAA 1 cut(s) 379
BbsI GAAGAC 1 cut(s) 476
Bbv12I GWGCWC 1 cut(s) 443
BbvCI CCTCAGC 1 cut(s) 323
BbvI GCAGC 4 cut(s) 57, 174, 383, 576
BccI CCATC 2 cut(s) 88, 128
BceAI ACGGC 1 cut(s) 538
BciT130I CCWGG 1 cut(s) 278
BclI TGATCA 3 cut(s) 214, 298, 517
BfmI CTRYAG 3 cut(s) 385, 397, 444
BfoI RGCGCY 1 cut(s) 45
BfrI CTTAAG 1 cut(s) 529
BisI GCNGC 5 cut(s) 71, 163, 224, 397, 590
BlsI GCNGC 5 cut(s) 72, 164, 225, 398, 591
Bme1390I CCNGG 1 cut(s) 278
BmiI GGNNCC 1 cut(s) 485
BmrFI CCNGG 1 cut(s) 278
BmsI GCATC 1 cut(s) 252
BpiI GAAGAC 1 cut(s) 476
Bpu10I CCTNAGC 1 cut(s) 323
Bpu14I TTCGAA 1 cut(s) 379
BsaJI CCNNGG 1 cut(s) 615
BsaWI WCCGGW 1 cut(s) 486
BsaXI ACNNNNNCTCC 4 cut(s) 481, 511, 587, 617
Bse118I RCCGGY 1 cut(s) 4
Bse3DI GCAATG 1 cut(s) 466
BseBI CCWGG 1 cut(s) 278
BseDI CCNNGG 1 cut(s) 615
BseGI GGATG 2 cut(s) 22, 139
BseMI GCAATG 1 cut(s) 466
BseMII CTCAG 1 cut(s) 314
BseRI GAGGAG 3 cut(s) 340, 568, 580
BseXI GCAGC 4 cut(s) 57, 174, 383, 576
BshFI GGCC 1 cut(s) 525
BsiHKAI GWGCWC 1 cut(s) 443
BsiSI CCGG 2 cut(s) 5, 487
BsnI GGCC 1 cut(s) 525
Bsp119I TTCGAA 1 cut(s) 379
Bsp1286I GDGCHC 1 cut(s) 443
Bsp143I GATC 3 cut(s) 214, 298, 517
BspACI CCGC 3 cut(s) 8, 224, 608
BspANI GGCC 1 cut(s) 525
BspCNI CTCAG 1 cut(s) 315
BspLI GGNNCC 1 cut(s) 485
BspMAI CTGCAG 1 cut(s) 401
BspT104I TTCGAA 1 cut(s) 379
BspTI CTTAAG 1 cut(s) 529
BsrDI GCAATG 1 cut(s) 466
BsrFI RCCGGY 1 cut(s) 4
BssAI RCCGGY 1 cut(s) 4
BssECI CCNNGG 1 cut(s) 615
BssMI GATC 3 cut(s) 214, 298, 517
BssT1I CCWWGG 1 cut(s) 615
Bst2UI CCWGG 1 cut(s) 278
Bst4CI ACNGT 2 cut(s) 389, 445
Bst6I CTCTTC 3 cut(s) 123, 300, 575
BstAFI CTTAAG 1 cut(s) 529
BstBI TTCGAA 1 cut(s) 379
BstC8I GCNNGC 1 cut(s) 6
BstDEI CTNAG 2 cut(s) 323, 621
BstF5I GGATG 2 cut(s) 22, 139
BstH2I RGCGCY 1 cut(s) 45
BstHHI GCGC 2 cut(s) 44, 494
BstKTI GATC 3 cut(s) 217, 301, 520
BstMBI GATC 3 cut(s) 214, 298, 517
BstMWI GCNNNNNNNGC 2 cut(s) 232, 353
BstNI CCWGG 1 cut(s) 278
BstSCI CCNGG 1 cut(s) 276
BstSFI CTRYAG 3 cut(s) 385, 397, 444
BstV1I GCAGC 4 cut(s) 57, 174, 383, 576
BstV2I GAAGAC 1 cut(s) 476
BsuRI GGCC 1 cut(s) 525
BtsCI GGATG 2 cut(s) 22, 139
BtsIMutI CAGTG 2 cut(s) 394, 441
Cac8I GCNNGC 1 cut(s) 6
CaiI CAGNNNCTG 1 cut(s) 373
CfoI GCGC 2 cut(s) 44, 494
Cfr10I RCCGGY 1 cut(s) 4
CspCI CAANNNNNGTGG 2 cut(s) 105, 140
CviAII CATG 1 cut(s) 287
DdeI CTNAG 2 cut(s) 323, 621
DpnI GATC 3 cut(s) 216, 300, 519
DpnII GATC 3 cut(s) 214, 298, 517
Eam1104I CTCTTC 3 cut(s) 123, 300, 575
EarI CTCTTC 3 cut(s) 123, 300, 575
EciI GGCGGA 1 cut(s) 23
Eco130I CCWWGG 1 cut(s) 615
EcoRI GAATTC 1 cut(s) 112
EcoRII CCWGG 1 cut(s) 276
EcoT14I CCWWGG 1 cut(s) 615
ErhI CCWWGG 1 cut(s) 615
FaeI CATG 1 cut(s) 290
FaiI YATR 4 cut(s) 60, 288, 480, 561
FatI CATG 1 cut(s) 286
FauI CCCGC 1 cut(s) 615
FbaI TGATCA 3 cut(s) 214, 298, 517
Fnu4HI GCNGC 5 cut(s) 71, 163, 224, 397, 590
FokI GGATG 2 cut(s) 29, 146
Fsp4HI GCNGC 5 cut(s) 71, 163, 224, 397, 590
GlaI GCGC 2 cut(s) 43, 493
GluI GCNGC 5 cut(s) 71, 163, 224, 397, 590
HaeII RGCGCY 1 cut(s) 45
HaeIII GGCC 1 cut(s) 525
HapII CCGG 2 cut(s) 5, 487
HhaI GCGC 2 cut(s) 44, 494
Hin1II CATG 1 cut(s) 290
Hin6I GCGC 2 cut(s) 42, 492
HinP1I GCGC 2 cut(s) 42, 492
HinfI GANTC 4 cut(s) 98, 308, 376, 572
HpaII CCGG 2 cut(s) 5, 487
HphI GGTGA 2 cut(s) 308, 495
Hpy188I TCNGA 1 cut(s) 219
Hpy188III TCNNGA 2 cut(s) 302, 515
Hpy99I CGWCG 1 cut(s) 112
HpyAV CCTTC 2 cut(s) 79, 164
HpyCH4III ACNGT 2 cut(s) 389, 445
HpyCH4IV ACGT 2 cut(s) 473, 496
HpyCH4V TGCA 4 cut(s) 265, 399, 411, 478
HpyF10VI GCNNNNNNNGC 2 cut(s) 232, 353
HpyF3I CTNAG 2 cut(s) 323, 621
HpySE526I ACGT 2 cut(s) 473, 496
Hsp92II CATG 1 cut(s) 290
HspAI GCGC 2 cut(s) 42, 492
KroI GCCGGC 1 cut(s) 4
KroNI GCCGGC 1 cut(s) 6
Ksp22I TGATCA 3 cut(s) 214, 298, 517
Kzo9I GATC 3 cut(s) 214, 298, 517
LmnI GCTCC 1 cut(s) 489
LpnPI CCDG 9 cut(s) 14, 18, 177, 238, 263, 290, 380, 421, 500
Lsp1109I GCAGC 4 cut(s) 57, 174, 383, 576
LweI GCATC 1 cut(s) 252
MaeII ACGT 2 cut(s) 473, 496
MaeIII GTNAC 1 cut(s) 497
MalI GATC 3 cut(s) 216, 300, 519
MboI GATC 3 cut(s) 214, 298, 517
MboII GAAGA 4 cut(s) 140, 317, 481, 592
MhlI GDGCHC 1 cut(s) 443
MluCI AATT 2 cut(s) 89, 112
MlyI GAGTC 2 cut(s) 107, 317
MroNI GCCGGC 1 cut(s) 4
MseI TTAA 1 cut(s) 530
MslI CAYNNNNRTG 1 cut(s) 291
MspA1I CMGCKG 1 cut(s) 396
MspCI CTTAAG 1 cut(s) 529
MspI CCGG 2 cut(s) 5, 487
MspR9I CCNGG 1 cut(s) 278
MvaI CCWGG 1 cut(s) 278
MwoI GCNNNNNNNGC 2 cut(s) 232, 353
NaeI GCCGGC 1 cut(s) 6
NdeII GATC 3 cut(s) 214, 298, 517
NgoMIV GCCGGC 1 cut(s) 4
NlaIII CATG 1 cut(s) 290
NlaIV GGNNCC 1 cut(s) 485
NspV TTCGAA 1 cut(s) 379
PcsI WCGNNNNNNNCGW 1 cut(s) 107
PdiI GCCGGC 1 cut(s) 6
PfeI GAWTC 2 cut(s) 376, 572
PkrI GCNGC 5 cut(s) 72, 164, 225, 398, 591
PleI GAGTC 2 cut(s) 106, 316
PpsI GAGTC 2 cut(s) 106, 316
Psp6I CCWGG 1 cut(s) 276
PspGI CCWGG 1 cut(s) 276
PspN4I GGNNCC 1 cut(s) 485
PstI CTGCAG 1 cut(s) 401
PstNI CAGNNNCTG 1 cut(s) 373
PvuII CAGCTG 1 cut(s) 396
RseI CAYNNNNRTG 1 cut(s) 291
SaqAI TTAA 1 cut(s) 530
SatI GCNGC 5 cut(s) 71, 163, 224, 397, 590
Sau3AI GATC 3 cut(s) 214, 298, 517
SchI GAGTC 2 cut(s) 107, 317
ScrFI CCNGG 1 cut(s) 278
SduI GDGCHC 1 cut(s) 443
SfaNI GCATC 1 cut(s) 252
SfcI CTRYAG 3 cut(s) 385, 397, 444
SfuI TTCGAA 1 cut(s) 379
SmiMI CAYNNNNRTG 1 cut(s) 291
SmlI CTYRAG 1 cut(s) 529
SmoI CTYRAG 1 cut(s) 529
Sse9I AATT 2 cut(s) 89, 112
SsiI CCGC 3 cut(s) 8, 224, 608
StyD4I CCNGG 1 cut(s) 276
StyI CCWWGG 1 cut(s) 615
TaaI ACNGT 2 cut(s) 389, 445
TaiI ACGT 2 cut(s) 476, 499
TaqI TCGA 5 cut(s) 35, 101, 110, 203, 379
TasI AATT 2 cut(s) 89, 112
TauI GCSGC 1 cut(s) 226
TfiI GAWTC 2 cut(s) 376, 572
Tru1I TTAA 1 cut(s) 530
Tru9I TTAA 1 cut(s) 530
TscAI CASTG 2 cut(s) 394, 448
TseI GCWGC 4 cut(s) 70, 162, 396, 589
TspGWI ACGGA 2 cut(s) 62, 95
TspRI CASTG 2 cut(s) 394, 448
Vha464I CTTAAG 1 cut(s) 529
XapI RAATTY 1 cut(s) 112
XcmI CCANNNNNNNNNTGG 1 cut(s) 284
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.