AT5G59770

Catalyzes the third of the four reactions of the long- chain fatty acids elongation cycle. This endoplasmic reticulum- bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0010281
Physical Location & Seq
Reverse (-)
24079297 .. 24081216
1920 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G59770.1

Sequence Viewer

Length: 666 bp
ATGTCGCCGTTTGTGAAGTTTTATCTCTTCAGTTACAATTTTCTCCAAGCTTCTGCATGGGCGATTTCGCTTCTGATTATTCTTAACAGCTTTTTATCGAACAAGACGATCATTTCTGCTTACGCTTCTGCTGGATTCCTGATTAGTCTGTTTCAAACTGCTGCTATTCTTGAAGTTCTTCATGGAGCTATTGGAATTGTCCCGAGTGGATTCTTGTCTCCACTGATGCAATGGAGTGGAAGAACTCACTTCATATTGGCTATTGTTGGACAGATCAAGGAGGTCCAGGATTCGCCGTGGCTGTCTATAACACTTGTGGCTTGGTGTATCGGTGAGATGATCAGATATCCTCATTATGCTTTTACTTGCCTCGGTAGATGTCCCTATTGGCTAACCTACCTCAGGTACACGGGATTCATCGTGATTTATCCAACGGGACTAGTGGGTGAATTGTTGATCATGTACAAAGCACTTCCATATGTTAAAGAAAGGAATCTTTACGCTAATTTCTTCTCTGTTTTCCCCTTCAGTTACTACGATTTCCTTTGGGCCGTCCTCTTGGTCTACCCATTCCTTTGGTTGAAGCTTTATCTCCAGCTTTTCAAACAAAGAAAGTCTAAGCTTGGAAAATCGAAGAAGCTTCACGGCAAGAGAAAGAGAATGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01040 ko01110 ko01212 map00062 map01040 map01110 map01212
Pfam Domains
Protein Families
PTPLA

Protein Analysis

221

Amino Acids

25.6

Weight (kDa)

9.79

Isoelectric Point (pI)

39.87

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTPLA PF04387 52 - 210 5.6e-52 Protein tyrosine phosphatase-like protein, PTPLA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccI GTMKAC 1 cut(s) 564
AcuI CTGAAG 2 cut(s) 13, 511
AfaI GTAC 2 cut(s) 407, 464
AfiI CCNNNNNNNGG 1 cut(s) 402
AgsI TTSAA 4 cut(s) 155, 173, 583, 604
AhlI ACTAGT 1 cut(s) 439
AjnI CCWGG 1 cut(s) 285
AluBI AGCT 7 cut(s) 50, 90, 188, 586, 598, 622, 640
AluI AGCT 7 cut(s) 50, 90, 188, 586, 598, 622, 640
Alw26I GTCTC 1 cut(s) 222
Ama87I CYCGRG 1 cut(s) 202
AoxI GGCC 1 cut(s) 549
ApeKI GCWGC 1 cut(s) 161
Asp700I GAANNNNTTC 1 cut(s) 177
AspS9I GGNCC 2 cut(s) 283, 549
AsuHPI GGTGA 2 cut(s) 344, 458
AvaI CYCGRG 1 cut(s) 202
AvaII GGWCC 1 cut(s) 283
AxyI CCTNAGG 1 cut(s) 401
BbvI GCAGC 1 cut(s) 148
BceAI ACGGC 3 cut(s) 280, 536, 661
BciT130I CCWGG 1 cut(s) 287
BclI TGATCA 2 cut(s) 339, 456
BcoDI GTCTC 1 cut(s) 222
BcuI ACTAGT 1 cut(s) 439
BfaI CTAG 1 cut(s) 440
BisI GCNGC 1 cut(s) 162
BlsI GCNGC 1 cut(s) 163
Bme1390I CCNGG 1 cut(s) 287
Bme18I GGWCC 1 cut(s) 283
BmeT110I CYCGRG 1 cut(s) 202
BmgT120I GGNCC 2 cut(s) 283, 549
BmrFI CCNGG 1 cut(s) 287
BmsI GCATC 1 cut(s) 216
BpmI CTGGAG 1 cut(s) 578
BsaJI CCNNGG 2 cut(s) 296, 370
Bsc4I CCNNNNNNNGG 1 cut(s) 402
Bse21I CCTNAGG 1 cut(s) 401
Bse3DI GCAATG 1 cut(s) 236
BseBI CCWGG 1 cut(s) 287
BseDI CCNNGG 2 cut(s) 296, 370
BseLI CCNNNNNNNGG 1 cut(s) 402
BseMI GCAATG 1 cut(s) 236
BseMII CTCAG 1 cut(s) 415
BseXI GCAGC 1 cut(s) 148
BshFI GGCC 1 cut(s) 551
BsiHKCI CYCGRG 1 cut(s) 202
BslFI GGGAC 3 cut(s) 185, 366, 450
BslI CCNNNNNNNGG 1 cut(s) 402
BsmAI GTCTC 1 cut(s) 222
BsmFI GGGAC 3 cut(s) 185, 366, 450
BsnI GGCC 1 cut(s) 551
BsoBI CYCGRG 1 cut(s) 202
Bsp1407I TGTACA 1 cut(s) 462
Bsp143I GATC 4 cut(s) 108, 273, 339, 456
BspANI GGCC 1 cut(s) 551
BspCNI CTCAG 1 cut(s) 414
BsrDI GCAATG 1 cut(s) 236
BsrGI TGTACA 1 cut(s) 462
BssECI CCNNGG 2 cut(s) 296, 370
BssMI GATC 4 cut(s) 108, 273, 339, 456
Bst2UI CCWGG 1 cut(s) 287
Bst6I CTCTTC 1 cut(s) 32
BstAUI TGTACA 1 cut(s) 462
BstDEI CTNAG 2 cut(s) 401, 618
BstDSI CCRYGG 1 cut(s) 296
BstENI CCTNNNNNAGG 1 cut(s) 400
BstKTI GATC 4 cut(s) 111, 276, 342, 459
BstMAI GTCTC 1 cut(s) 222
BstMBI GATC 4 cut(s) 108, 273, 339, 456
BstNI CCWGG 1 cut(s) 287
BstSCI CCNGG 1 cut(s) 285
BstV1I GCAGC 1 cut(s) 148
BstXI CCANNNNNNTGG 1 cut(s) 576
Bsu36I CCTNAGG 1 cut(s) 401
BsuRI GGCC 1 cut(s) 551
BtgI CCRYGG 1 cut(s) 296
BtsIMutI CAGTG 1 cut(s) 221
Cfr13I GGNCC 2 cut(s) 283, 549
Csp6I GTAC 2 cut(s) 406, 463
CviAII CATG 3 cut(s) 57, 182, 460
CviQI GTAC 2 cut(s) 406, 463
DdeI CTNAG 2 cut(s) 401, 618
DpnI GATC 4 cut(s) 110, 275, 341, 458
DpnII GATC 4 cut(s) 108, 273, 339, 456
Eam1104I CTCTTC 1 cut(s) 32
EarI CTCTTC 1 cut(s) 32
Eco32I GATATC 1 cut(s) 347
Eco47I GGWCC 1 cut(s) 283
Eco57I CTGAAG 2 cut(s) 13, 511
Eco81I CCTNAGG 1 cut(s) 401
Eco88I CYCGRG 1 cut(s) 202
EcoNI CCTNNNNNAGG 1 cut(s) 400
EcoRII CCWGG 1 cut(s) 285
EcoRV GATATC 1 cut(s) 347
FaeI CATG 3 cut(s) 60, 185, 463
FaiI YATR 8 cut(s) 58, 183, 254, 308, 357, 461, 478, 480
FaqI GGGAC 3 cut(s) 185, 366, 450
FatI CATG 3 cut(s) 56, 181, 459
FauNDI CATATG 1 cut(s) 478
FbaI TGATCA 2 cut(s) 339, 456
FblI GTMKAC 1 cut(s) 564
Fnu4HI GCNGC 1 cut(s) 162
Fsp4HI GCNGC 1 cut(s) 162
FspBI CTAG 1 cut(s) 440
GluI GCNGC 1 cut(s) 162
GsuI CTGGAG 1 cut(s) 578
HaeIII GGCC 1 cut(s) 551
Hin1II CATG 3 cut(s) 60, 185, 463
HindIII AAGCTT 4 cut(s) 48, 584, 620, 638
HinfI GANTC 5 cut(s) 135, 210, 290, 414, 493
HphI GGTGA 2 cut(s) 344, 458
Hpy166II GTNNAC 2 cut(s) 408, 565
Hpy188I TCNGA 2 cut(s) 75, 344
Hpy188III TCNNGA 4 cut(s) 139, 170, 202, 421
Hpy8I GTNNAC 2 cut(s) 408, 565
HpyAV CCTTC 1 cut(s) 535
HpyCH4V TGCA 2 cut(s) 56, 229
HpyF3I CTNAG 2 cut(s) 401, 618
Hsp92II CATG 3 cut(s) 60, 185, 463
Ksp22I TGATCA 2 cut(s) 339, 456
Kzo9I GATC 4 cut(s) 108, 273, 339, 456
LmnI GCTCC 1 cut(s) 185
LpnPI CCDG 6 cut(s) 117, 152, 272, 299, 388, 608
Lsp1109I GCAGC 1 cut(s) 148
LweI GCATC 1 cut(s) 216
MaeI CTAG 1 cut(s) 440
MaeIII GTNAC 2 cut(s) 32, 530
MalI GATC 4 cut(s) 110, 275, 341, 458
MboI GATC 4 cut(s) 108, 273, 339, 456
MboII GAAGA 5 cut(s) 19, 170, 252, 502, 646
MluCI AATT 4 cut(s) 37, 195, 449, 505
MmeI TCCRAC 2 cut(s) 247, 455
MnlI CCTC 5 cut(s) 274, 360, 380, 410, 566
MroXI GAANNNNTTC 1 cut(s) 177
MseI TTAA 2 cut(s) 84, 483
MspR9I CCNGG 1 cut(s) 287
MvaI CCWGG 1 cut(s) 287
NdeI CATATG 1 cut(s) 478
NdeII GATC 4 cut(s) 108, 273, 339, 456
NlaIII CATG 3 cut(s) 60, 185, 463
PcsI WCGNNNNNNNCGW 1 cut(s) 104
PdmI GAANNNNTTC 1 cut(s) 177
PfeI GAWTC 5 cut(s) 135, 210, 290, 414, 493
PfoI TCCNGGA 1 cut(s) 285
PkrI GCNGC 1 cut(s) 163
Psp6I CCWGG 1 cut(s) 285
PspGI CCWGG 1 cut(s) 285
PspPI GGNCC 2 cut(s) 283, 549
RsaI GTAC 2 cut(s) 407, 464
RsaNI GTAC 2 cut(s) 406, 463
SaqAI TTAA 2 cut(s) 84, 483
SatI GCNGC 1 cut(s) 162
Sau3AI GATC 4 cut(s) 108, 273, 339, 456
Sau96I GGNCC 2 cut(s) 283, 549
ScrFI CCNGG 1 cut(s) 287
SfaNI GCATC 1 cut(s) 216
SinI GGWCC 1 cut(s) 283
SpeI ACTAGT 1 cut(s) 439
Sse9I AATT 4 cut(s) 37, 195, 449, 505
SspMI CTAG 1 cut(s) 440
StyD4I CCNGG 1 cut(s) 285
TaqI TCGA 2 cut(s) 98, 632
TasI AATT 4 cut(s) 37, 195, 449, 505
TatI WGTACW 1 cut(s) 462
TfiI GAWTC 5 cut(s) 135, 210, 290, 414, 493
Tru1I TTAA 2 cut(s) 84, 483
Tru9I TTAA 2 cut(s) 84, 483
TscAI CASTG 1 cut(s) 228
TseI GCWGC 1 cut(s) 161
TspDTI ATGAA 3 cut(s) 170, 241, 406
TspRI CASTG 1 cut(s) 228
VpaK11BI GGWCC 1 cut(s) 283
XagI CCTNNNNNAGG 1 cut(s) 400
XcmI CCANNNNNNNNNTGG 1 cut(s) 228
XmiI GTMKAC 1 cut(s) 564
XmnI GAANNNNTTC 1 cut(s) 177
XspI CTAG 1 cut(s) 440
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.