Catalyzes the third of the four reactions of the long- chain fatty acids elongation cycle. This endoplasmic reticulum- bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators
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| Species | Orthologous Gene IDs |
|---|---|
| arabidopsis_thaliana | AT5G59770 |
| fragaria_vesca | FvH4_3g34950 FvH4_3g34950 |
| malus_domestica | MD03G1100000.v1.1 |
| prunus_persica | Prupe.6G085100_v2.0.a1 Prupe.6G085100_v2.0.a1 Prupe.6G085100_v2.0.a1 |
| pyrus_communis | pycom03g08230 pycom11g09600 |
| rosa_chinensis | RchiOBHm_Chr5g0062801 |
| rosa_laevigata | RLG00000035581 |
| rosa_multiflora | Rmu_sc0014744.1_g000001 Rmu_sc0026275.1_g000001 |
| rosa_roxburghii | Rroxscaffold_1G00017820 |
| rosa_rugosa | Rorug05G0351700 Rorug05G0351800 |
| rosa_samantha | Rh5AG413000 Rh5BG427000 Rh5CG450800 Rh5DG440800 |
| rosa_wichuraiana | Rw5G038810 |
| Enzyme | Recognition Site | Cut Count | Positions (bp) |
|---|---|---|---|
| AanI | TTATAA | 1 cut(s) | 26 |
| AciI | CCGC | 1 cut(s) | 101 |
| AcuI | CTGAAG | 1 cut(s) | 199 |
| AfaI | GTAC | 3 cut(s) | 125, 182, 209 |
| AfiI | CCNNNNNNNGG | 1 cut(s) | 158 |
| AgsI | TTSAA | 2 cut(s) | 82, 202 |
| AjnI | CCWGG | 2 cut(s) | 151, 170 |
| AluBI | AGCT | 1 cut(s) | 219 |
| AluI | AGCT | 1 cut(s) | 219 |
| AsuHPI | GGTGA | 1 cut(s) | 176 |
| BccI | CCATC | 1 cut(s) | 151 |
| BciT130I | CCWGG | 2 cut(s) | 153, 172 |
| BciVI | GTATCC | 1 cut(s) | 156 |
| BfuI | GTATCC | 1 cut(s) | 156 |
| BmcAI | AGTACT | 1 cut(s) | 209 |
| Bme1390I | CCNGG | 2 cut(s) | 153, 172 |
| BmrFI | CCNGG | 2 cut(s) | 153, 172 |
| BmsI | GCATC | 2 cut(s) | 54, 96 |
| BsaJI | CCNNGG | 2 cut(s) | 104, 152 |
| BsaXI | ACNNNNNCTCC | 2 cut(s) | 34, 64 |
| Bsc4I | CCNNNNNNNGG | 1 cut(s) | 158 |
| BseBI | CCWGG | 2 cut(s) | 153, 172 |
| BseDI | CCNNGG | 2 cut(s) | 104, 152 |
| BseGI | GGATG | 1 cut(s) | 162 |
| BseLI | CCNNNNNNNGG | 1 cut(s) | 158 |
| BseMII | CTCAG | 1 cut(s) | 133 |
| BslI | CCNNNNNNNGG | 1 cut(s) | 158 |
| BsmI | GAATGC | 1 cut(s) | 131 |
| BspACI | CCGC | 1 cut(s) | 101 |
| BspCNI | CTCAG | 1 cut(s) | 132 |
| BssECI | CCNNGG | 2 cut(s) | 104, 152 |
| BssT1I | CCWWGG | 1 cut(s) | 104 |
| Bst2UI | CCWGG | 2 cut(s) | 153, 172 |
| Bst4CI | ACNGT | 1 cut(s) | 9 |
| BstC8I | GCNNGC | 1 cut(s) | 15 |
| BstDEI | CTNAG | 1 cut(s) | 119 |
| BstF5I | GGATG | 1 cut(s) | 162 |
| BstNI | CCWGG | 2 cut(s) | 153, 172 |
| BstSCI | CCNGG | 2 cut(s) | 151, 170 |
| BsuI | GTATCC | 1 cut(s) | 156 |
| BtsCI | GGATG | 1 cut(s) | 162 |
| BtsI | GCAGTG | 1 cut(s) | 188 |
| BtsIMutI | CAGTG | 2 cut(s) | 55, 188 |
| Cac8I | GCNNGC | 1 cut(s) | 15 |
| Csp6I | GTAC | 3 cut(s) | 124, 181, 208 |
| CviAII | CATG | 2 cut(s) | 39, 185 |
| CviJI | RGCY | 2 cut(s) | 109, 219 |
| CviKI_1 | RGCY | 2 cut(s) | 109, 219 |
| CviQI | GTAC | 3 cut(s) | 124, 181, 208 |
| DdeI | CTNAG | 1 cut(s) | 119 |
| EciI | GGCGGA | 1 cut(s) | 90 |
| Eco130I | CCWWGG | 1 cut(s) | 104 |
| Eco32I | GATATC | 1 cut(s) | 65 |
| Eco57I | CTGAAG | 1 cut(s) | 199 |
| EcoRII | CCWGG | 2 cut(s) | 151, 170 |
| EcoRV | GATATC | 1 cut(s) | 65 |
| EcoT14I | CCWWGG | 1 cut(s) | 104 |
| ErhI | CCWWGG | 1 cut(s) | 104 |
| FaeI | CATG | 2 cut(s) | 42, 188 |
| FaiI | YATR | 6 cut(s) | 26, 40, 75, 179, 186, 225 |
| FalI | AAGNNNNNCTT | 2 cut(s) | 197, 229 |
| FatI | CATG | 2 cut(s) | 38, 184 |
| FokI | GGATG | 1 cut(s) | 169 |
| Hin1II | CATG | 2 cut(s) | 42, 188 |
| HphI | GGTGA | 1 cut(s) | 176 |
| Hpy188I | TCNGA | 2 cut(s) | 92, 234 |
| HpyAV | CCTTC | 1 cut(s) | 223 |
| HpyCH4III | ACNGT | 1 cut(s) | 9 |
| HpyCH4V | TGCA | 4 cut(s) | 38, 87, 131, 188 |
| HpyF3I | CTNAG | 1 cut(s) | 119 |
| Hsp92II | CATG | 2 cut(s) | 42, 188 |
| LmnI | GCTCC | 1 cut(s) | 42 |
| LpnPI | CCDG | 6 cut(s) | 27, 106, 138, 157, 165, 184 |
| LweI | GCATC | 2 cut(s) | 54, 96 |
| MboII | GAAGA | 1 cut(s) | 214 |
| MluCI | AATT | 2 cut(s) | 70, 82 |
| MmeI | TCCRAC | 1 cut(s) | 28 |
| MnlI | CCTC | 2 cut(s) | 46, 159 |
| MspR9I | CCNGG | 2 cut(s) | 153, 172 |
| Mva1269I | GAATGC | 1 cut(s) | 131 |
| MvaI | CCWGG | 2 cut(s) | 153, 172 |
| NlaIII | CATG | 2 cut(s) | 42, 188 |
| PctI | GAATGC | 1 cut(s) | 131 |
| PsiI | TTATAA | 1 cut(s) | 26 |
| Psp6I | CCWGG | 2 cut(s) | 151, 170 |
| PspGI | CCWGG | 2 cut(s) | 151, 170 |
| RsaI | GTAC | 3 cut(s) | 125, 182, 209 |
| RsaNI | GTAC | 3 cut(s) | 124, 181, 208 |
| ScaI | AGTACT | 1 cut(s) | 209 |
| ScrFI | CCNGG | 2 cut(s) | 153, 172 |
| SetI | ASST | 4 cut(s) | 57, 125, 173, 221 |
| SfaNI | GCATC | 2 cut(s) | 54, 96 |
| Sse9I | AATT | 2 cut(s) | 70, 82 |
| SsiI | CCGC | 1 cut(s) | 101 |
| StyD4I | CCNGG | 2 cut(s) | 151, 170 |
| StyI | CCWWGG | 1 cut(s) | 104 |
| TaaI | ACNGT | 1 cut(s) | 9 |
| TasI | AATT | 2 cut(s) | 70, 82 |
| TatI | WGTACW | 1 cut(s) | 207 |
| TscAI | CASTG | 2 cut(s) | 55, 195 |
| TspRI | CASTG | 2 cut(s) | 55, 195 |
| ZrmI | AGTACT | 1 cut(s) | 209 |