Rorug05G0351700

Catalyzes the third of the four reactions of the long- chain fatty acids elongation cycle. This endoplasmic reticulum- bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_rugosa
GWHBQTZ00000005
OG0010281
Physical Location & Seq
Forward (+)
43874373 .. 43882297
7925 bp
Loading structure...
UTR
Exon/CDS
Intron
Rorug05G0351700.1

Sequence Viewer

Length: 1137 bp
ATGAAGAAGGTGATCTCCCTGATTCTGATGGGCTGCGGAGGCGTTGGCCGTCAGCTCCTCCAACACATGGTCTCCTGCCGACCCCTTCACGCCAAGCAGGGAATCCACCTGAGAGTTGTGGGAGTATCTGACAGTAAATCCTTAGTTGTTGCGGCCGACGTGTCGACTATGGAGTTGGCTGATAATTCTTTATTGCAACTTTGCCGGATCAAGTCCGGTGGTTCTTCTTCTCTGCACACGCTTGCCGCCTCTGGTCAAGTAGAGGTATTCACCAGTTCTGAATCAACCAAAAAGATTATCGATGTTGCTGCACTCTTGGGTAAATCAACAGGTTTGGCTCTTGTAGATTGCTCTGCGAGTTCTGAGACTATTCCACTGCTCACCCAAGCAATTGATTTGGGATGTTGCATTGTCATGGCAAACAAGAAGCCTCTTACTTCAACAATGGAGGATTTTGACAAACTAATTTCACACCCACGTCACATACGTCATGAGTCAACTGTTGGTGCCGGCCTTCCTGTCATATCATCCTTGCATCGCATTCTTTCATCTGGAGATCCCGTCCGCTGCATTATTGGGAGTTTGAGTGGAACATTGGGGTATGTTATGAGTGAGGTCGAAGATGGAAAGCCAATTAGTGAAGTTGTGCGGTCTGCTAAAAATCTGGGTTACACTGAACCAGATCCACGTGATGACCTCAGTGGGATGGATGTTGCAAGAAAGGCTTTGATACTTGCTAGGCTTCTCGGTTGGCGTATTAATTTGGATGACATTAAGATTGAGAGCTTGTATCCTAAAGAAATGGCACCCGGTGTAATGTCTGTGGAAGAATTTCTGGGAACTGGGCTACTATTGCTTGATAAAGATATCAAAGAGAGAGTCTCTAAAGCTTCTTTGAGCGGGAATGTGCTACGTTATGTCTGTGTGATTAAGGCCTCAAGGTGTGAAGTTGGCATTCAAGAGCTTGCAAAGGATTCTCCTTTAGGAAGACTGAGAGGAAGTGACAATGTGCTGGAGATTTACAGTAGATGTTATAATGACCAACCACTGGTCATTCAAGGAGCTGGAGCTGGCAATGATACTACTGCTGCTGGAGTCCTTGCTGATATCATTGATATTCAAGATTTATTTCCCTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01040 ko01110 ko01212 map00062 map01040 map01110 map01212
Pfam Domains
Protein Families
PTPLA

Protein Analysis

378

Amino Acids

40.33

Weight (kDa)

6.26

Isoelectric Point (pI)

33.64

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Homoserine_dh PF00742 173 - 371 3.8e-47 Homoserine dehydrogenase
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 1035
AccB1I GGYRCC 2 cut(s) 506, 805
AccB7I CCANNNNNTGG 2 cut(s) 67, 1048
AccBSI CCGCTC 1 cut(s) 900
AccI GTMKAC 1 cut(s) 164
AciI CCGC 6 cut(s) 36, 152, 246, 565, 649, 900
AclWI GGATC 3 cut(s) 215, 551, 677
AcoI YGGCCR 2 cut(s) 46, 153
AcsI RAATTY 1 cut(s) 830
AcvI CACGTG 1 cut(s) 689
AdeI CACNNNGTG 1 cut(s) 812
AfiI CCNNNNNNNGG 2 cut(s) 67, 1048
AflIII ACRYGT 1 cut(s) 159
AgsI TTSAA 4 cut(s) 441, 959, 1058, 1121
AjiI CACGTC 2 cut(s) 160, 479
AluBI AGCT 6 cut(s) 55, 786, 890, 964, 1064, 1070
AluI AGCT 6 cut(s) 55, 786, 890, 964, 1064, 1070
Alw26I GTCTC 3 cut(s) 76, 359, 886
AlwI GGATC 3 cut(s) 215, 551, 677
AoxI GGCC 4 cut(s) 46, 153, 511, 933
ApeKI GCWGC 4 cut(s) 33, 308, 567, 1088
ApoI RAATTY 1 cut(s) 830
ArsI GACNNNNNNTTYG 2 cut(s) 864, 896
AseI ATTAAT 1 cut(s) 759
Asp700I GAANNNNTTC 1 cut(s) 831
AsuC2I CCSGG 1 cut(s) 810
AsuHPI GGTGA 3 cut(s) 22, 262, 373
BanI GGYRCC 2 cut(s) 506, 805
BbrPI CACGTG 1 cut(s) 689
BbsI GAAGAC 1 cut(s) 994
BbvI GCAGC 4 cut(s) 20, 295, 554, 1075
BccI CCATC 3 cut(s) 22, 617, 700
BceAI ACGGC 1 cut(s) 33
BcgI CGANNNNNNTGC 2 cut(s) 290, 324
BciVI GTATCC 1 cut(s) 801
BcnI CCSGG 1 cut(s) 810
BcoDI GTCTC 3 cut(s) 76, 359, 886
BfaI CTAG 1 cut(s) 738
BfuI GTATCC 1 cut(s) 801
BisI GCNGC 6 cut(s) 34, 153, 246, 309, 568, 1089
BlsI GCNGC 6 cut(s) 35, 154, 247, 310, 569, 1090
Bme1390I CCNGG 1 cut(s) 810
BmgBI CACGTC 2 cut(s) 160, 479
BmiI GGNNCC 2 cut(s) 508, 807
BmrFI CCNGG 1 cut(s) 810
BmrI ACTGGG 1 cut(s) 852
BmsI GCATC 1 cut(s) 544
BmuI ACTGGG 1 cut(s) 852
BpiI GAAGAC 1 cut(s) 994
BplI GAGNNNNNCTC 2 cut(s) 866, 898
BpmI CTGGAG 4 cut(s) 573, 1034, 1086, 1113
BpuEI CTTGAG 1 cut(s) 922
BpuMI CCSGG 1 cut(s) 810
Bsa29I ATCGAT 1 cut(s) 300
BsaAI YACGTR 1 cut(s) 689
BsaI GGTCTC 1 cut(s) 76
BsaWI WCCGGW 1 cut(s) 215
BsaXI ACNNNNNCTCC 6 cut(s) 56, 86, 546, 571, 576, 601
Bsc4I CCNNNNNNNGG 2 cut(s) 67, 1048
Bse118I RCCGGY 1 cut(s) 509
Bse1I ACTGG 3 cut(s) 273, 847, 1053
Bse3DI GCAATG 1 cut(s) 1081
BseCI ATCGAT 1 cut(s) 300
BseGI GGATG 5 cut(s) 407, 527, 711, 715, 772
BseLI CCNNNNNNNGG 2 cut(s) 67, 1048
BseMI GCAATG 1 cut(s) 1081
BseMII CTCAG 4 cut(s) 101, 354, 712, 983
BseNI ACTGG 3 cut(s) 273, 847, 1053
BseRI GAGGAG 1 cut(s) 47
BseX3I CGGCCG 1 cut(s) 153
BseXI GCAGC 4 cut(s) 20, 295, 554, 1075
BsgI GTGCAG 2 cut(s) 218, 294
Bsh1285I CGRYCG 1 cut(s) 156
BshFI GGCC 4 cut(s) 48, 155, 513, 935
BshNI GGYRCC 2 cut(s) 506, 805
BshVI ATCGAT 1 cut(s) 300
BsiEI CGRYCG 1 cut(s) 156
BsiSI CCGG 4 cut(s) 205, 216, 510, 810
BslI CCNNNNNNNGG 2 cut(s) 67, 1048
BsmAI GTCTC 3 cut(s) 76, 359, 886
BsmI GAATGC 2 cut(s) 540, 954
BsnI GGCC 4 cut(s) 48, 155, 513, 935
Bso31I GGTCTC 1 cut(s) 76
Bsp143I GATC 4 cut(s) 12, 207, 556, 682
BspACI CCGC 6 cut(s) 36, 152, 246, 565, 649, 900
BspANI GGCC 4 cut(s) 48, 155, 513, 935
BspCNI CTCAG 4 cut(s) 102, 355, 711, 984
BspDI ATCGAT 1 cut(s) 300
BspHI TCATGA 1 cut(s) 490
BspLI GGNNCC 2 cut(s) 508, 807
BspPI GGATC 3 cut(s) 215, 551, 677
BspT107I GGYRCC 2 cut(s) 506, 805
BspTNI GGTCTC 1 cut(s) 76
BsrBI CCGCTC 1 cut(s) 900
BsrDI GCAATG 1 cut(s) 1081
BsrFI RCCGGY 1 cut(s) 509
BsrI ACTGG 3 cut(s) 273, 847, 1053
BssAI RCCGGY 1 cut(s) 509
BssMI GATC 4 cut(s) 12, 207, 556, 682
Bst4CI ACNGT 3 cut(s) 134, 502, 1025
BstBAI YACGTR 1 cut(s) 689
BstC8I GCNNGC 4 cut(s) 243, 511, 966, 1072
BstDEI CTNAG 5 cut(s) 110, 142, 363, 698, 992
BstF5I GGATG 5 cut(s) 407, 527, 711, 715, 772
BstKTI GATC 4 cut(s) 15, 210, 559, 685
BstMAI GTCTC 3 cut(s) 76, 359, 886
BstMBI GATC 4 cut(s) 12, 207, 556, 682
BstMCI CGRYCG 1 cut(s) 156
BstMWI GCNNNNNNNGC 3 cut(s) 39, 722, 853
BstSCI CCNGG 1 cut(s) 808
BstV1I GCAGC 4 cut(s) 20, 295, 554, 1075
BstV2I GAAGAC 1 cut(s) 994
BstX2I RGATCY 2 cut(s) 556, 682
BstYI RGATCY 2 cut(s) 556, 682
BstZI CGGCCG 1 cut(s) 153
Bsu15I ATCGAT 1 cut(s) 300
BsuI GTATCC 1 cut(s) 801
BsuRI GGCC 4 cut(s) 48, 155, 513, 935
BsuTUI ATCGAT 1 cut(s) 300
BtgZI GCGATG 1 cut(s) 521
BtrI CACGTC 2 cut(s) 160, 479
BtsCI GGATG 5 cut(s) 407, 527, 711, 715, 772
BtsI GCAGTG 1 cut(s) 374
BtsIMutI CAGTG 4 cut(s) 374, 672, 706, 1046
Cac8I GCNNGC 4 cut(s) 243, 511, 966, 1072
CciI TCATGA 1 cut(s) 490
Cfr10I RCCGGY 1 cut(s) 509
ClaI ATCGAT 1 cut(s) 300
CspCI CAANNNNNGTGG 2 cut(s) 199, 234
CviAII CATG 3 cut(s) 67, 415, 491
DdeI CTNAG 5 cut(s) 110, 142, 363, 698, 992
DpnI GATC 4 cut(s) 14, 209, 558, 684
DpnII GATC 4 cut(s) 12, 207, 556, 682
DraIII CACNNNGTG 1 cut(s) 812
EaeI YGGCCR 2 cut(s) 46, 153
EagI CGGCCG 1 cut(s) 153
EclXI CGGCCG 1 cut(s) 153
Eco147I AGGCCT 1 cut(s) 935
Eco31I GGTCTC 1 cut(s) 76
Eco32I GATATC 2 cut(s) 868, 1108
Eco52I CGGCCG 1 cut(s) 153
Eco72I CACGTG 1 cut(s) 689
EcoRV GATATC 2 cut(s) 868, 1108
FaeI CATG 3 cut(s) 70, 418, 494
FalI AAGNNNNNCTT 2 cut(s) 709, 741
FatI CATG 3 cut(s) 66, 414, 490
FauI CCCGC 1 cut(s) 893
FblI GTMKAC 1 cut(s) 164
Fnu4HI GCNGC 6 cut(s) 34, 153, 246, 309, 568, 1089
FokI GGATG 5 cut(s) 414, 514, 718, 722, 779
Fsp4HI GCNGC 6 cut(s) 34, 153, 246, 309, 568, 1089
FspBI CTAG 1 cut(s) 738
GluI GCNGC 6 cut(s) 34, 153, 246, 309, 568, 1089
GsuI CTGGAG 4 cut(s) 573, 1034, 1086, 1113
HaeIII GGCC 4 cut(s) 48, 155, 513, 935
HapII CCGG 4 cut(s) 205, 216, 510, 810
Hin1II CATG 3 cut(s) 70, 418, 494
HincII GTYRAC 2 cut(s) 165, 498
HindII GTYRAC 2 cut(s) 165, 498
HindIII AAGCTT 1 cut(s) 888
HinfI GANTC 7 cut(s) 22, 102, 281, 494, 879, 974, 1095
HpaII CCGG 4 cut(s) 205, 216, 510, 810
HphI GGTGA 3 cut(s) 22, 262, 373
Hpy166II GTNNAC 2 cut(s) 165, 498
Hpy188I TCNGA 4 cut(s) 27, 130, 280, 364
Hpy188III TCNNGA 4 cut(s) 491, 552, 959, 1121
Hpy8I GTNNAC 2 cut(s) 165, 498
Hpy99I CGWCG 1 cut(s) 161
HpyAV CCTTC 2 cut(s) 95, 524
HpyCH4III ACNGT 3 cut(s) 134, 502, 1025
HpyCH4IV ACGT 5 cut(s) 159, 478, 487, 688, 913
HpyCH4V TGCA 8 cut(s) 196, 235, 311, 408, 535, 570, 716, 968
HpyF10VI GCNNNNNNNGC 3 cut(s) 39, 722, 853
HpyF3I CTNAG 5 cut(s) 110, 142, 363, 698, 992
HpySE526I ACGT 5 cut(s) 159, 478, 487, 688, 913
Hsp92II CATG 3 cut(s) 70, 418, 494
KroI GCCGGC 1 cut(s) 509
KroNI GCCGGC 1 cut(s) 511
Kzo9I GATC 4 cut(s) 12, 207, 556, 682
LmnI GCTCC 3 cut(s) 60, 1061, 1067
Lsp1109I GCAGC 4 cut(s) 20, 295, 554, 1075
LweI GCATC 1 cut(s) 544
MaeI CTAG 1 cut(s) 738
MaeII ACGT 5 cut(s) 159, 478, 487, 688, 913
MaeIII GTNAC 3 cut(s) 479, 668, 1001
MalI GATC 4 cut(s) 14, 209, 558, 684
MbiI CCGCTC 1 cut(s) 900
MboI GATC 4 cut(s) 12, 207, 556, 682
MboII GAAGA 6 cut(s) 16, 216, 219, 632, 839, 999
MfeI CAATTG 1 cut(s) 390
MflI RGATCY 2 cut(s) 556, 682
MluCI AATT 6 cut(s) 184, 390, 465, 633, 760, 830
MlyI GAGTC 3 cut(s) 503, 888, 1104
MmeI TCCRAC 1 cut(s) 85
MroNI GCCGGC 1 cut(s) 509
MroXI GAANNNNTTC 1 cut(s) 831
MseI TTAA 3 cut(s) 759, 774, 930
MslI CAYNNNNRTG 1 cut(s) 413
MspA1I CMGCKG 1 cut(s) 567
MspI CCGG 4 cut(s) 205, 216, 510, 810
MspR9I CCNGG 1 cut(s) 810
MunI CAATTG 1 cut(s) 390
Mva1269I GAATGC 2 cut(s) 540, 954
MwoI GCNNNNNNNGC 3 cut(s) 39, 722, 853
NaeI GCCGGC 1 cut(s) 511
NciI CCSGG 1 cut(s) 810
NdeII GATC 4 cut(s) 12, 207, 556, 682
NgoMIV GCCGGC 1 cut(s) 509
NlaIII CATG 3 cut(s) 70, 418, 494
NlaIV GGNNCC 2 cut(s) 508, 807
NmuCI GTSAC 2 cut(s) 479, 1001
PagI TCATGA 1 cut(s) 490
PceI AGGCCT 1 cut(s) 935
PcsI WCGNNNNNNNCGW 1 cut(s) 484
PctI GAATGC 2 cut(s) 540, 954
PdiI GCCGGC 1 cut(s) 511
PdmI GAANNNNTTC 1 cut(s) 831
PfeI GAWTC 4 cut(s) 22, 102, 281, 974
PflMI CCANNNNNTGG 2 cut(s) 67, 1048
PkrI GCNGC 6 cut(s) 35, 154, 247, 310, 569, 1090
PleI GAGTC 3 cut(s) 502, 887, 1103
PmaCI CACGTG 1 cut(s) 689
PmlI CACGTG 1 cut(s) 689
PpsI GAGTC 3 cut(s) 502, 887, 1103
Ppu21I YACGTR 1 cut(s) 689
PshBI ATTAAT 1 cut(s) 759
PsiI TTATAA 1 cut(s) 1035
PspCI CACGTG 1 cut(s) 689
PspN4I GGNNCC 2 cut(s) 508, 807
PsuI RGATCY 2 cut(s) 556, 682
RseI CAYNNNNRTG 1 cut(s) 413
SalI GTCGAC 1 cut(s) 163
SaqAI TTAA 3 cut(s) 759, 774, 930
SatI GCNGC 6 cut(s) 34, 153, 246, 309, 568, 1089
Sau3AI GATC 4 cut(s) 12, 207, 556, 682
SchI GAGTC 3 cut(s) 503, 888, 1104
ScrFI CCNGG 1 cut(s) 810
SfaNI GCATC 1 cut(s) 544
SmiMI CAYNNNNRTG 1 cut(s) 413
SmlI CTYRAG 1 cut(s) 937
SmoI CTYRAG 1 cut(s) 937
Sse9I AATT 6 cut(s) 184, 390, 465, 633, 760, 830
SseBI AGGCCT 1 cut(s) 935
SsiI CCGC 6 cut(s) 36, 152, 246, 565, 649, 900
SspMI CTAG 1 cut(s) 738
StuI AGGCCT 1 cut(s) 935
StyD4I CCNGG 1 cut(s) 808
TaaI ACNGT 3 cut(s) 134, 502, 1025
TaiI ACGT 5 cut(s) 162, 481, 490, 691, 916
TaqI TCGA 3 cut(s) 164, 300, 618
TasI AATT 6 cut(s) 184, 390, 465, 633, 760, 830
TauI GCSGC 2 cut(s) 155, 248
TfiI GAWTC 4 cut(s) 22, 102, 281, 974
Tru1I TTAA 3 cut(s) 759, 774, 930
Tru9I TTAA 3 cut(s) 759, 774, 930
TscAI CASTG 4 cut(s) 381, 679, 706, 1053
TseFI GTSAC 2 cut(s) 479, 1001
TseI GCWGC 4 cut(s) 33, 308, 567, 1088
Tsp45I GTSAC 2 cut(s) 479, 1001
TspDTI ATGAA 2 cut(s) 17, 537
TspRI CASTG 4 cut(s) 381, 679, 706, 1053
Van91I CCANNNNNTGG 2 cut(s) 67, 1048
VspI ATTAAT 1 cut(s) 759
XapI RAATTY 1 cut(s) 830
XmiI GTMKAC 1 cut(s) 164
XmnI GAANNNNTTC 1 cut(s) 831
XspI CTAG 1 cut(s) 738
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.