FvH4_3g34950

Catalyzes the third of the four reactions of the long- chain fatty acids elongation cycle. This endoplasmic reticulum- bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb3
OG0010281
Physical Location & Seq
Reverse (-)
30165793 .. 30169921
4129 bp
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UTR
Exon/CDS
Intron
FvH4_3g34950.t1

Sequence Viewer

Length: 621 bp
ATGCCTTCTCTATCCAATCTCTATCTCTTGGCCTACAACTCTGCTCAAGCCATTGCATGGGCAGTTTGTCTCGCTCTAAGCTTGGGCAGTGTTCTATCCACCAAGACTCTCAATGGTGCCTATGCTTCTGCTGGAGACCTCATCTATTTGTGCCAGATGGTTGCGTTCTTGGAAGTTGTACACGGAGCCGTTGGTCTGGTGCCGAGTGGAGTGGTGATTCCTCTGATGCAATGGGCAGGGAGGACAAATTGTATGTTTGTAATGCGTCAAACCCATGAGGTCCAAGAGTTGCCAGCAGTGTTGATAATTTTTGTTGCATGGAGCATAAGTGAGATTATTAGATATCCAACCTATGCTTTGAATTGCATCAGAAGTTGTCCACCTTGGCTTACCTATCTCAGGTACTCTGCATTCCTTGTCTTGTATCCTCCTGGGATGGGTGGTGAAACCTGGATCATGTACTATGCACTTCCATTTATGAAGAAATACTCCTTCGGTTATTATAGGTTTCTGATGGTTGTGCTTTTCTGCTACCCGTTCCTCTGTTTGAAAGTTTACTTGCATATGGTCAAGCAACGGCGATCAAAGCTAGGTAAACAAGACAAGAAGAAGAAGAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01040 ko01110 ko01212 map00062 map01040 map01110 map01212
Pfam Domains
Protein Families
PTPLA

Protein Analysis

207

Amino Acids

23.46

Weight (kDa)

9.51

Isoelectric Point (pI)

40.79

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTPLA PF04387 52 - 199 7.3e-47 Protein tyrosine phosphatase-like protein, PTPLA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB1I GGYRCC 2 cut(s) 116, 199
AccB7I CCANNNNNTGG 1 cut(s) 57
AclWI GGATC 1 cut(s) 461
AfaI GTAC 3 cut(s) 180, 404, 461
AfiI CCNNNNNNNGG 3 cut(s) 57, 399, 437
AgsI TTSAA 2 cut(s) 361, 550
AjnI CCWGG 2 cut(s) 430, 449
AluBI AGCT 2 cut(s) 81, 589
AluI AGCT 2 cut(s) 81, 589
Alw26I GTCTC 2 cut(s) 74, 129
AlwI GGATC 1 cut(s) 461
AoxI GGCC 1 cut(s) 30
AspS9I GGNCC 1 cut(s) 280
AsuHPI GGTGA 2 cut(s) 226, 455
AvaII GGWCC 1 cut(s) 280
BanI GGYRCC 2 cut(s) 116, 199
BccI CCATC 3 cut(s) 151, 430, 508
BceAI ACGGC 2 cut(s) 173, 593
BciT130I CCWGG 2 cut(s) 432, 451
BciVI GTATCC 1 cut(s) 435
BcoDI GTCTC 2 cut(s) 74, 129
BfaI CTAG 1 cut(s) 590
BfuI GTATCC 1 cut(s) 435
Bme1390I CCNGG 2 cut(s) 432, 451
Bme18I GGWCC 1 cut(s) 280
BmgT120I GGNCC 1 cut(s) 280
BmiI GGNNCC 3 cut(s) 118, 187, 201
BmrFI CCNGG 2 cut(s) 432, 451
BmsI GCATC 2 cut(s) 216, 375
BpmI CTGGAG 1 cut(s) 153
BpuEI CTTGAG 1 cut(s) 30
BsaI GGTCTC 1 cut(s) 129
BsaJI CCNNGG 2 cut(s) 383, 431
BsaXI ACNNNNNCTCC 2 cut(s) 313, 343
Bsc4I CCNNNNNNNGG 3 cut(s) 57, 399, 437
Bse3DI GCAATG 2 cut(s) 51, 236
BseBI CCWGG 2 cut(s) 432, 451
BseDI CCNNGG 2 cut(s) 383, 431
BseGI GGATG 1 cut(s) 441
BseLI CCNNNNNNNGG 3 cut(s) 57, 399, 437
BseMI GCAATG 2 cut(s) 51, 236
BseMII CTCAG 1 cut(s) 412
BshFI GGCC 1 cut(s) 32
BshNI GGYRCC 2 cut(s) 116, 199
BslI CCNNNNNNNGG 3 cut(s) 57, 399, 437
BsmAI GTCTC 2 cut(s) 74, 129
BsmI GAATGC 1 cut(s) 410
BsnI GGCC 1 cut(s) 32
Bso31I GGTCTC 1 cut(s) 129
Bsp1407I TGTACA 1 cut(s) 178
Bsp143I GATC 2 cut(s) 453, 581
BspANI GGCC 1 cut(s) 32
BspCNI CTCAG 1 cut(s) 411
BspLI GGNNCC 3 cut(s) 118, 187, 201
BspPI GGATC 1 cut(s) 461
BspT107I GGYRCC 2 cut(s) 116, 199
BspTNI GGTCTC 1 cut(s) 129
BsrDI GCAATG 2 cut(s) 51, 236
BsrGI TGTACA 1 cut(s) 178
BssECI CCNNGG 2 cut(s) 383, 431
BssMI GATC 2 cut(s) 453, 581
BssT1I CCWWGG 1 cut(s) 383
Bst2UI CCWGG 2 cut(s) 432, 451
Bst6I CTCTTC 1 cut(s) 608
BstAUI TGTACA 1 cut(s) 178
BstC8I GCNNGC 1 cut(s) 294
BstDEI CTNAG 2 cut(s) 77, 398
BstENI CCTNNNNNAGG 1 cut(s) 397
BstF5I GGATG 1 cut(s) 441
BstKTI GATC 2 cut(s) 456, 584
BstMAI GTCTC 2 cut(s) 74, 129
BstMBI GATC 2 cut(s) 453, 581
BstMWI GCNNNNNNNGC 1 cut(s) 586
BstNI CCWGG 2 cut(s) 432, 451
BstSCI CCNGG 2 cut(s) 430, 449
BsuI GTATCC 1 cut(s) 435
BsuRI GGCC 1 cut(s) 32
BtsCI GGATG 1 cut(s) 441
BtsI GCAGTG 2 cut(s) 94, 303
BtsIMutI CAGTG 2 cut(s) 94, 303
Cac8I GCNNGC 1 cut(s) 294
Cfr13I GGNCC 1 cut(s) 280
CseI GACGC 1 cut(s) 254
Csp6I GTAC 3 cut(s) 179, 403, 460
CviAII CATG 4 cut(s) 57, 275, 318, 457
CviJI RGCY 6 cut(s) 32, 50, 81, 188, 388, 589
CviKI_1 RGCY 6 cut(s) 32, 50, 81, 188, 388, 589
CviQI GTAC 3 cut(s) 179, 403, 460
DdeI CTNAG 2 cut(s) 77, 398
DpnI GATC 2 cut(s) 455, 583
DpnII GATC 2 cut(s) 453, 581
Eam1104I CTCTTC 1 cut(s) 608
EarI CTCTTC 1 cut(s) 608
Eco130I CCWWGG 1 cut(s) 383
Eco31I GGTCTC 1 cut(s) 129
Eco32I GATATC 1 cut(s) 344
Eco47I GGWCC 1 cut(s) 280
EcoNI CCTNNNNNAGG 1 cut(s) 397
EcoRII CCWGG 2 cut(s) 430, 449
EcoRV GATATC 1 cut(s) 344
EcoT14I CCWWGG 1 cut(s) 383
ErhI CCWWGG 1 cut(s) 383
FaeI CATG 4 cut(s) 60, 278, 321, 460
FatI CATG 4 cut(s) 56, 274, 317, 456
FauNDI CATATG 1 cut(s) 564
FokI GGATG 1 cut(s) 448
FspBI CTAG 1 cut(s) 590
GsuI CTGGAG 1 cut(s) 153
HaeIII GGCC 1 cut(s) 32
HgaI GACGC 1 cut(s) 254
Hin1II CATG 4 cut(s) 60, 278, 321, 460
HindIII AAGCTT 1 cut(s) 79
HinfI GANTC 2 cut(s) 106, 217
HphI GGTGA 2 cut(s) 226, 455
Hpy166II GTNNAC 4 cut(s) 181, 380, 556, 596
Hpy188I TCNGA 3 cut(s) 225, 371, 513
Hpy8I GTNNAC 4 cut(s) 181, 380, 556, 596
HpyAV CCTTC 2 cut(s) 15, 502
HpyCH4V TGCA 7 cut(s) 56, 229, 317, 366, 410, 467, 562
HpyF10VI GCNNNNNNNGC 1 cut(s) 586
HpyF3I CTNAG 2 cut(s) 77, 398
Hsp92II CATG 4 cut(s) 60, 278, 321, 460
Kzo9I GATC 2 cut(s) 453, 581
LmnI GCTCC 2 cut(s) 185, 321
LweI GCATC 2 cut(s) 216, 375
MaeI CTAG 1 cut(s) 590
MalI GATC 2 cut(s) 455, 583
MboI GATC 2 cut(s) 453, 581
MboII GAAGA 2 cut(s) 493, 619
MluCI AATT 3 cut(s) 247, 306, 361
MlyI GAGTC 1 cut(s) 100
MmeI TCCRAC 1 cut(s) 371
MnlI CCTC 7 cut(s) 149, 231, 234, 271, 438, 551, 609
MspR9I CCNGG 2 cut(s) 432, 451
Mva1269I GAATGC 1 cut(s) 410
MvaI CCWGG 2 cut(s) 432, 451
MwoI GCNNNNNNNGC 1 cut(s) 586
NdeI CATATG 1 cut(s) 564
NdeII GATC 2 cut(s) 453, 581
NlaIII CATG 4 cut(s) 60, 278, 321, 460
NlaIV GGNNCC 3 cut(s) 118, 187, 201
NmeAIII GCCGAG 1 cut(s) 228
PctI GAATGC 1 cut(s) 410
PfeI GAWTC 1 cut(s) 217
PflMI CCANNNNNTGG 1 cut(s) 57
PleI GAGTC 1 cut(s) 100
PpsI GAGTC 1 cut(s) 100
Psp6I CCWGG 2 cut(s) 430, 449
PspGI CCWGG 2 cut(s) 430, 449
PspN4I GGNNCC 3 cut(s) 118, 187, 201
PspPI GGNCC 1 cut(s) 280
RsaI GTAC 3 cut(s) 180, 404, 461
RsaNI GTAC 3 cut(s) 179, 403, 460
Sau3AI GATC 2 cut(s) 453, 581
Sau96I GGNCC 1 cut(s) 280
SchI GAGTC 1 cut(s) 100
ScrFI CCNGG 2 cut(s) 432, 451
SfaNI GCATC 2 cut(s) 216, 375
SinI GGWCC 1 cut(s) 280
SmlI CTYRAG 1 cut(s) 45
SmoI CTYRAG 1 cut(s) 45
Sse9I AATT 3 cut(s) 247, 306, 361
SspMI CTAG 1 cut(s) 590
StyD4I CCNGG 2 cut(s) 430, 449
StyI CCWWGG 1 cut(s) 383
TasI AATT 3 cut(s) 247, 306, 361
TatI WGTACW 2 cut(s) 178, 459
TfiI GAWTC 1 cut(s) 217
TscAI CASTG 2 cut(s) 94, 303
TspDTI ATGAA 1 cut(s) 494
TspGWI ACGGA 1 cut(s) 198
TspRI CASTG 2 cut(s) 94, 303
Van91I CCANNNNNTGG 1 cut(s) 57
VpaK11BI GGWCC 1 cut(s) 280
XagI CCTNNNNNAGG 1 cut(s) 397
XspI CTAG 1 cut(s) 590
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.