pycom03g08230

Catalyzes the third of the four reactions of the long- chain fatty acids elongation cycle. This endoplasmic reticulum- bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
pyrus_communis
Chr3
OG0010281
Physical Location & Seq
Forward (+)
6634371 .. 6638218
3848 bp
Loading structure...
UTR
Exon/CDS
Intron
pycom03g08230.7

Sequence Viewer

Length: 771 bp
ATGTACGTGGCGAGCCGGACCTTGGGAGGCATACCGGTGACGACTCCCTTGGTGTACCCGAGAGTCTACGCCAGAAAAACGTACCACCCGGCGGCGATCGGTCCGCTCCAACCGTTGAAAATCGGAAAGCCAGAAATGGCAAAGCTATCCAATCTCTATCTCTTGGCTTACAACTCTCTTCAGGCTGCTGCATGGGCAGTTTGTCTCTCTCTAAGCTTGAGCAGCGCCATCAATGGCGCCTACGCTTCTGCTGGACACCTTATGTATTTGCTCCAAATGGTTCAGTTCTTGGAAGTTATACACGGAGCCATTGGTCTGGTGCCGAGTGGGGTGGTGTTTCCTCTGATGCAATGGGCAGGAAGGGCGCATTTTCTGTATGTTGTGCGCCAAACCCATGAGGTCCAGGAGTCGCCATCAGTCTTCATAACTTTTGTTGCTTGGAGCCTAAGTGAGGTTATTAGGTATCCACATTATGCTTTTAATTGCAGGGGGAGCTGTCCATCTTGGATTACCTATCTCAGGTACACTGCATTCATTGTTTTGTATCCTCCAGGGATGGCTGGTGAAACCTGGCTTATGTACCAAGCACTTCCTTTTATAAAGAAGAAGAGCCTCTCCCCAGATTTCTCCACTGGCCTCTCCTTCAGCTATTATAACTTTCTGAGGGTTTTGCTTGTCTGCTACCCATTCCTCTGCTTGAAACTTTACCTGCATATGTTCAAGCAACGCCGATCAAAACTGGGTAAGCACCCCAAGAAGAAGAAAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01040 ko01110 ko01212 map00062 map01040 map01110 map01212
Pfam Domains
Protein Families
PTPLA

Protein Analysis

257

Amino Acids

29.02

Weight (kDa)

9.87

Isoelectric Point (pI)

41.68

Instability Index
Protein Domains (Pfam)
No Pfam domains detected for this protein.
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Publication-ready
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 599, 654
Acc36I ACCTGC 1 cut(s) 717
AccB1I GGYRCC 2 cut(s) 236, 319
AccBSI CCGCTC 1 cut(s) 106
AccI GTMKAC 1 cut(s) 66
AciI CCGC 2 cut(s) 92, 104
AcuI CTGAAG 2 cut(s) 164, 628
AcyI GRCGYC 1 cut(s) 237
AfaI GTAC 5 cut(s) 5, 56, 83, 524, 581
AfiI CCNNNNNNNGG 4 cut(s) 22, 91, 451, 519
AgeI ACCGGT 1 cut(s) 34
AgsI TTSAA 3 cut(s) 118, 700, 721
AjnI CCWGG 3 cut(s) 402, 550, 569
AluBI AGCT 4 cut(s) 145, 216, 495, 648
AluI AGCT 4 cut(s) 145, 216, 495, 648
Alw26I GTCTC 1 cut(s) 209
Ama87I CYCGRG 1 cut(s) 58
AoxI GGCC 1 cut(s) 634
ApeKI GCWGC 3 cut(s) 185, 188, 222
AsiGI ACCGGT 1 cut(s) 34
AspLEI GCGC 4 cut(s) 227, 239, 367, 387
AspS9I GGNCC 3 cut(s) 18, 101, 400
AsuC2I CCSGG 1 cut(s) 89
AsuHPI GGTGA 2 cut(s) 49, 575
AvaI CYCGRG 1 cut(s) 58
AvaII GGWCC 3 cut(s) 18, 101, 400
BanI GGYRCC 2 cut(s) 236, 319
BbsI GAAGAC 1 cut(s) 412
BbvI GCAGC 3 cut(s) 172, 175, 234
BccI CCATC 4 cut(s) 236, 421, 508, 550
BciT130I CCWGG 3 cut(s) 404, 552, 571
BciVI GTATCC 2 cut(s) 474, 555
BcnI CCSGG 1 cut(s) 89
BcoDI GTCTC 1 cut(s) 209
BfoI RGCGCY 2 cut(s) 228, 240
BfuAI ACCTGC 1 cut(s) 717
BfuI GTATCC 2 cut(s) 474, 555
BisI GCNGC 4 cut(s) 93, 186, 189, 223
BlsI GCNGC 4 cut(s) 94, 187, 190, 224
Bme1390I CCNGG 4 cut(s) 89, 404, 552, 571
Bme18I GGWCC 3 cut(s) 18, 101, 400
BmeT110I CYCGRG 1 cut(s) 58
BmgT120I GGNCC 3 cut(s) 18, 101, 400
BmiI GGNNCC 4 cut(s) 238, 307, 321, 443
BmrFI CCNGG 4 cut(s) 89, 404, 552, 571
BmrI ACTGGG 1 cut(s) 749
BmsI GCATC 1 cut(s) 336
BmuI ACTGGG 1 cut(s) 749
BpiI GAAGAC 1 cut(s) 412
BpmI CTGGAG 1 cut(s) 534
BpuEI CTTGAG 1 cut(s) 238
BpuMI CCSGG 1 cut(s) 89
BsaAI YACGTR 1 cut(s) 7
BsaHI GRCGYC 1 cut(s) 237
BsaJI CCNNGG 3 cut(s) 21, 48, 551
BsaWI WCCGGW 1 cut(s) 34
BsaXI ACNNNNNCTCC 2 cut(s) 433, 463
Bsc4I CCNNNNNNNGG 4 cut(s) 22, 91, 451, 519
Bse118I RCCGGY 1 cut(s) 34
Bse1I ACTGG 2 cut(s) 637, 744
Bse3DI GCAATG 1 cut(s) 356
BseBI CCWGG 3 cut(s) 404, 552, 571
BseDI CCNNGG 3 cut(s) 21, 48, 551
BseGI GGATG 1 cut(s) 561
BseLI CCNNNNNNNGG 4 cut(s) 22, 91, 451, 519
BseMI GCAATG 1 cut(s) 356
BseMII CTCAG 2 cut(s) 532, 653
BseNI ACTGG 2 cut(s) 637, 744
BseXI GCAGC 3 cut(s) 172, 175, 234
Bsh1285I CGRYCG 1 cut(s) 99
BshFI GGCC 1 cut(s) 636
BshNI GGYRCC 2 cut(s) 236, 319
BshTI ACCGGT 1 cut(s) 34
BsiEI CGRYCG 1 cut(s) 99
BsiHKCI CYCGRG 1 cut(s) 58
BsiSI CCGG 3 cut(s) 16, 35, 89
BslI CCNNNNNNNGG 4 cut(s) 22, 91, 451, 519
BsmAI GTCTC 1 cut(s) 209
BsmI GAATGC 1 cut(s) 530
BsnI GGCC 1 cut(s) 636
BsoBI CYCGRG 1 cut(s) 58
Bsp143I GATC 2 cut(s) 96, 731
BspACI CCGC 2 cut(s) 92, 104
BspANI GGCC 1 cut(s) 636
BspCNI CTCAG 2 cut(s) 531, 654
BspLI GGNNCC 4 cut(s) 238, 307, 321, 443
BspMI ACCTGC 1 cut(s) 717
BspQI GCTCTTC 1 cut(s) 602
BspT107I GGYRCC 2 cut(s) 236, 319
BsrBI CCGCTC 1 cut(s) 106
BsrDI GCAATG 1 cut(s) 356
BsrFI RCCGGY 1 cut(s) 34
BsrI ACTGG 2 cut(s) 637, 744
BssAI RCCGGY 1 cut(s) 34
BssECI CCNNGG 3 cut(s) 21, 48, 551
BssMI GATC 2 cut(s) 96, 731
BssNI GRCGYC 1 cut(s) 237
BssT1I CCWWGG 2 cut(s) 21, 48
Bst2UI CCWGG 3 cut(s) 404, 552, 571
Bst4CI ACNGT 1 cut(s) 114
Bst6I CTCTTC 2 cut(s) 183, 602
BstACI GRCGYC 1 cut(s) 237
BstBAI YACGTR 1 cut(s) 7
BstC8I GCNNGC 1 cut(s) 13
BstDEI CTNAG 4 cut(s) 212, 446, 518, 662
BstENI CCTNNNNNAGG 2 cut(s) 449, 517
BstF5I GGATG 1 cut(s) 561
BstH2I RGCGCY 2 cut(s) 228, 240
BstHHI GCGC 4 cut(s) 227, 239, 367, 387
BstKTI GATC 2 cut(s) 99, 734
BstMAI GTCTC 1 cut(s) 209
BstMBI GATC 2 cut(s) 96, 731
BstMCI CGRYCG 1 cut(s) 99
BstMWI GCNNNNNNNGC 4 cut(s) 194, 222, 362, 492
BstNI CCWGG 3 cut(s) 404, 552, 571
BstSCI CCNGG 4 cut(s) 87, 402, 550, 569
BstV1I GCAGC 3 cut(s) 172, 175, 234
BstV2I GAAGAC 1 cut(s) 412
BstXI CCANNNNNNTGG 1 cut(s) 316
BsuI GTATCC 2 cut(s) 474, 555
BsuRI GGCC 1 cut(s) 636
BtsCI GGATG 1 cut(s) 561
BtsI GCAGTG 1 cut(s) 525
BtsIMutI CAGTG 2 cut(s) 525, 630
BveI ACCTGC 1 cut(s) 717
Cac8I GCNNGC 1 cut(s) 13
CfoI GCGC 4 cut(s) 227, 239, 367, 387
Cfr10I RCCGGY 1 cut(s) 34
Cfr13I GGNCC 3 cut(s) 18, 101, 400
CpoI CGGWCCG 1 cut(s) 101
Csp6I GTAC 5 cut(s) 4, 55, 82, 523, 580
CspAI ACCGGT 1 cut(s) 34
CspI CGGWCCG 1 cut(s) 101
CviAII CATG 2 cut(s) 192, 395
CviQI GTAC 5 cut(s) 4, 55, 82, 523, 580
DdeI CTNAG 4 cut(s) 212, 446, 518, 662
DinI GGCGCC 1 cut(s) 238
DpnI GATC 2 cut(s) 98, 733
DpnII GATC 2 cut(s) 96, 731
Eam1104I CTCTTC 2 cut(s) 183, 602
EarI CTCTTC 2 cut(s) 183, 602
Eco130I CCWWGG 2 cut(s) 21, 48
Eco47I GGWCC 3 cut(s) 18, 101, 400
Eco57I CTGAAG 2 cut(s) 164, 628
Eco88I CYCGRG 1 cut(s) 58
EcoNI CCTNNNNNAGG 2 cut(s) 449, 517
EcoRII CCWGG 3 cut(s) 402, 550, 569
EcoT14I CCWWGG 2 cut(s) 21, 48
EgeI GGCGCC 1 cut(s) 238
EheI GGCGCC 1 cut(s) 238
ErhI CCWWGG 2 cut(s) 21, 48
FaeI CATG 2 cut(s) 195, 398
FatI CATG 2 cut(s) 191, 394
FauNDI CATATG 1 cut(s) 714
FblI GTMKAC 1 cut(s) 66
Fnu4HI GCNGC 4 cut(s) 93, 186, 189, 223
FokI GGATG 1 cut(s) 568
Fsp4HI GCNGC 4 cut(s) 93, 186, 189, 223
GlaI GCGC 4 cut(s) 226, 238, 366, 386
GluI GCNGC 4 cut(s) 93, 186, 189, 223
GsuI CTGGAG 1 cut(s) 534
HaeII RGCGCY 2 cut(s) 228, 240
HaeIII GGCC 1 cut(s) 636
HapII CCGG 3 cut(s) 16, 35, 89
HhaI GCGC 4 cut(s) 227, 239, 367, 387
Hin1I GRCGYC 1 cut(s) 237
Hin1II CATG 2 cut(s) 195, 398
Hin6I GCGC 4 cut(s) 225, 237, 365, 385
HinP1I GCGC 4 cut(s) 225, 237, 365, 385
HindIII AAGCTT 1 cut(s) 214
HinfI GANTC 3 cut(s) 43, 63, 407
HpaII CCGG 3 cut(s) 16, 35, 89
HphI GGTGA 2 cut(s) 49, 575
Hpy166II GTNNAC 3 cut(s) 55, 67, 525
Hpy188I TCNGA 3 cut(s) 125, 345, 663
Hpy8I GTNNAC 3 cut(s) 55, 67, 525
HpyAV CCTTC 2 cut(s) 354, 652
HpyCH4III ACNGT 1 cut(s) 114
HpyCH4IV ACGT 2 cut(s) 6, 80
HpyCH4V TGCA 5 cut(s) 191, 349, 486, 530, 712
HpyF10VI GCNNNNNNNGC 4 cut(s) 194, 222, 362, 492
HpyF3I CTNAG 4 cut(s) 212, 446, 518, 662
HpySE526I ACGT 2 cut(s) 6, 80
Hsp92I GRCGYC 1 cut(s) 237
Hsp92II CATG 2 cut(s) 195, 398
HspAI GCGC 4 cut(s) 225, 237, 365, 385
KasI GGCGCC 1 cut(s) 236
Kzo9I GATC 2 cut(s) 96, 731
LguI GCTCTTC 1 cut(s) 602
LmnI GCTCC 5 cut(s) 111, 276, 305, 441, 492
Lsp1109I GCAGC 3 cut(s) 172, 175, 234
LweI GCATC 1 cut(s) 336
MaeII ACGT 2 cut(s) 6, 80
MaeIII GTNAC 1 cut(s) 37
MalI GATC 2 cut(s) 98, 733
MbiI CCGCTC 1 cut(s) 106
MboI GATC 2 cut(s) 96, 731
MboII GAAGA 5 cut(s) 170, 412, 616, 619, 769
MluCI AATT 1 cut(s) 481
Mly113I GGCGCC 1 cut(s) 237
MlyI GAGTC 3 cut(s) 37, 72, 416
MmeI TCCRAC 1 cut(s) 133
MnlI CCTC 9 cut(s) 20, 351, 391, 445, 558, 623, 647, 657, 701
MseI TTAA 1 cut(s) 480
MslI CAYNNNNRTG 1 cut(s) 35
MspI CCGG 3 cut(s) 16, 35, 89
MspR9I CCNGG 4 cut(s) 89, 404, 552, 571
Mva1269I GAATGC 1 cut(s) 530
MvaI CCWGG 3 cut(s) 404, 552, 571
MwoI GCNNNNNNNGC 4 cut(s) 194, 222, 362, 492
NarI GGCGCC 1 cut(s) 237
NciI CCSGG 1 cut(s) 89
NdeI CATATG 1 cut(s) 714
NdeII GATC 2 cut(s) 96, 731
NlaIII CATG 2 cut(s) 195, 398
NlaIV GGNNCC 4 cut(s) 238, 307, 321, 443
NmeAIII GCCGAG 1 cut(s) 348
NmuCI GTSAC 1 cut(s) 37
PciSI GCTCTTC 1 cut(s) 602
PctI GAATGC 1 cut(s) 530
PfoI TCCNGGA 1 cut(s) 402
PinAI ACCGGT 1 cut(s) 34
PkrI GCNGC 4 cut(s) 94, 187, 190, 224
Ple19I CGATCG 1 cut(s) 99
PleI GAGTC 3 cut(s) 37, 71, 415
PluTI GGCGCC 1 cut(s) 240
PpsI GAGTC 3 cut(s) 37, 71, 415
Ppu21I YACGTR 1 cut(s) 7
PsiI TTATAA 2 cut(s) 599, 654
Psp6I CCWGG 3 cut(s) 402, 550, 569
PspGI CCWGG 3 cut(s) 402, 550, 569
PspN4I GGNNCC 4 cut(s) 238, 307, 321, 443
PspPI GGNCC 3 cut(s) 18, 101, 400
PvuI CGATCG 1 cut(s) 99
RsaI GTAC 5 cut(s) 5, 56, 83, 524, 581
RsaNI GTAC 5 cut(s) 4, 55, 82, 523, 580
RseI CAYNNNNRTG 1 cut(s) 35
Rsr2I CGGWCCG 1 cut(s) 101
RsrII CGGWCCG 1 cut(s) 101
SapI GCTCTTC 1 cut(s) 602
SaqAI TTAA 1 cut(s) 480
SatI GCNGC 4 cut(s) 93, 186, 189, 223
Sau3AI GATC 2 cut(s) 96, 731
Sau96I GGNCC 3 cut(s) 18, 101, 400
SchI GAGTC 3 cut(s) 37, 72, 416
ScrFI CCNGG 4 cut(s) 89, 404, 552, 571
SfaNI GCATC 1 cut(s) 336
SfoI GGCGCC 1 cut(s) 238
SinI GGWCC 3 cut(s) 18, 101, 400
SmiMI CAYNNNNRTG 1 cut(s) 35
SmlI CTYRAG 1 cut(s) 217
SmoI CTYRAG 1 cut(s) 217
Sse9I AATT 1 cut(s) 481
SsiI CCGC 2 cut(s) 92, 104
SspDI GGCGCC 1 cut(s) 236
StyD4I CCNGG 4 cut(s) 87, 402, 550, 569
StyI CCWWGG 2 cut(s) 21, 48
TaaI ACNGT 1 cut(s) 114
TaiI ACGT 2 cut(s) 9, 83
TaqII GACCGA 1 cut(s) 89
TasI AATT 1 cut(s) 481
TauI GCSGC 1 cut(s) 95
Tru1I TTAA 1 cut(s) 480
Tru9I TTAA 1 cut(s) 480
TscAI CASTG 2 cut(s) 532, 637
TseFI GTSAC 1 cut(s) 37
TseI GCWGC 3 cut(s) 185, 188, 222
Tsp45I GTSAC 1 cut(s) 37
TspDTI ATGAA 2 cut(s) 412, 523
TspGWI ACGGA 1 cut(s) 318
TspRI CASTG 2 cut(s) 532, 637
VpaK11BI GGWCC 3 cut(s) 18, 101, 400
XagI CCTNNNNNAGG 2 cut(s) 449, 517
XmiI GTMKAC 1 cut(s) 66
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.