MD03G1100000.v1.1

Catalyzes the third of the four reactions of the long- chain fatty acids elongation cycle. This endoplasmic reticulum- bound enzymatic process, allows the addition of two carbons to the chain of long- and very long-chain fatty acids VLCFAs per cycle. This enzyme catalyzes the dehydration of the 3-hydroxyacyl-CoA intermediate into trans-2,3-enoyl-CoA, within each cycle of fatty acid elongation. Thereby, it participates to the production of VLCFAs of different chain lengths that are involved in multiple biological processes as precursors of membrane lipids and lipid mediators

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
malus_domestica
Chr03
OG0010281
Physical Location & Seq
Forward (+)
8558846 .. 8563322
4477 bp
Loading structure...
UTR
Exon/CDS
Intron
MD03G1100000.v1.1.491

Sequence Viewer

Length: 636 bp
ATGGCAAGGCTATCCAATCTCTATCTCTTGGCTTACAACTCTCTTCAGGCTGCTGCATGGGCAGTTTGTCTCTCTCTAAGCTTGAGCAGTGCCGTCAATGGCGCCTACGCTTCTGCTGGACACCTTATCTATTTGCTCCAAATGGTTCAGTTCTTGGAAGTTATACACGGAGCCATTGGTCTGGTGCCGAGTGGAGTTGTGTTTCCTCTGATGCAATGGGCAGGAAGGGCGCATTTTCTGTACGTTGTGCGCCAAACCCATGAGGTCCAGGAGTCGCCATCAGTCTTCATAACTTTTGTTGCTTGGAGCCTAAGTGAGGTTATTAGGTATCCACATTATGCTTTTAATTGCACAGGGAGCTGTCCATCTTGGATTACCTATCTCAGGTACACTGCATTCATTGTCTTGTATCCTCCAGGGATGGCTGGTGAAACCTGGCTTATGTACCAAGCACTTCCTTTTATAAAGAAGAAGAGCCTCTCCCCAGATTTCCCCGCTGGCCTCTCCTTCAGCTATTATAACTTTCTGAGGGTTTTGCTTGTCTGCTACCCATTCCTCTGCTTGAAACTTTACCTGCATATGTTCAAGCAACGCCAATCAAAACTGGGTAAGCACCCCAAGAAGAAGAAAAGGTGA
Functional Annotation
Gene Ontology
Molecular Function
Biological Process
Cellular Component
No ontology terms assigned.
KEGG Pathways
Metabolic & Signaling
ko00062 ko01040 ko01110 ko01212 map00062 map01040 map01110 map01212
Pfam Domains
Protein Families
PTPLA

Protein Analysis

212

Amino Acids

24.01

Weight (kDa)

9.64

Isoelectric Point (pI)

43.38

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
PTPLA PF04387 47 - 204 1.3e-53 Protein tyrosine phosphatase-like protein, PTPLA
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 2 cut(s) 464, 519
Acc36I ACCTGC 1 cut(s) 582
AccB1I GGYRCC 2 cut(s) 101, 184
AciI CCGC 1 cut(s) 495
AcuI CTGAAG 2 cut(s) 29, 493
AcyI GRCGYC 1 cut(s) 102
AfaI GTAC 3 cut(s) 242, 389, 446
AfiI CCNNNNNNNGG 2 cut(s) 316, 384
AgsI TTSAA 2 cut(s) 565, 586
AjnI CCWGG 3 cut(s) 267, 415, 434
AluBI AGCT 3 cut(s) 81, 360, 513
AluI AGCT 3 cut(s) 81, 360, 513
Alw26I GTCTC 1 cut(s) 74
AoxI GGCC 1 cut(s) 499
ApeKI GCWGC 2 cut(s) 50, 53
AspLEI GCGC 3 cut(s) 104, 232, 252
AspS9I GGNCC 1 cut(s) 265
AsuHPI GGTGA 1 cut(s) 440
AvaII GGWCC 1 cut(s) 265
BanI GGYRCC 2 cut(s) 101, 184
BbsI GAAGAC 1 cut(s) 277
BbvI GCAGC 2 cut(s) 37, 40
BccI CCATC 3 cut(s) 286, 373, 415
BceAI ACGGC 1 cut(s) 77
BciT130I CCWGG 3 cut(s) 269, 417, 436
BciVI GTATCC 2 cut(s) 339, 420
BcoDI GTCTC 1 cut(s) 74
BfoI RGCGCY 1 cut(s) 105
BfuAI ACCTGC 1 cut(s) 582
BfuI GTATCC 2 cut(s) 339, 420
BisI GCNGC 2 cut(s) 51, 54
BlsI GCNGC 2 cut(s) 52, 55
Bme1390I CCNGG 3 cut(s) 269, 417, 436
Bme18I GGWCC 1 cut(s) 265
BmgT120I GGNCC 1 cut(s) 265
BmiI GGNNCC 4 cut(s) 103, 172, 186, 308
BmrFI CCNGG 3 cut(s) 269, 417, 436
BmrI ACTGGG 1 cut(s) 614
BmsI GCATC 1 cut(s) 201
BmuI ACTGGG 1 cut(s) 614
BpiI GAAGAC 1 cut(s) 277
BpmI CTGGAG 1 cut(s) 399
BpuEI CTTGAG 1 cut(s) 103
BsaHI GRCGYC 1 cut(s) 102
BsaJI CCNNGG 1 cut(s) 416
BsaXI ACNNNNNCTCC 2 cut(s) 298, 328
Bsc4I CCNNNNNNNGG 2 cut(s) 316, 384
Bse1I ACTGG 1 cut(s) 609
Bse3DI GCAATG 1 cut(s) 221
BseBI CCWGG 3 cut(s) 269, 417, 436
BseDI CCNNGG 1 cut(s) 416
BseGI GGATG 1 cut(s) 426
BseLI CCNNNNNNNGG 2 cut(s) 316, 384
BseMI GCAATG 1 cut(s) 221
BseMII CTCAG 2 cut(s) 397, 518
BseNI ACTGG 1 cut(s) 609
BseXI GCAGC 2 cut(s) 37, 40
BshFI GGCC 1 cut(s) 501
BshNI GGYRCC 2 cut(s) 101, 184
BslI CCNNNNNNNGG 2 cut(s) 316, 384
BsmAI GTCTC 1 cut(s) 74
BsmI GAATGC 1 cut(s) 395
BsnI GGCC 1 cut(s) 501
BspACI CCGC 1 cut(s) 495
BspANI GGCC 1 cut(s) 501
BspCNI CTCAG 2 cut(s) 396, 519
BspLI GGNNCC 4 cut(s) 103, 172, 186, 308
BspMI ACCTGC 1 cut(s) 582
BspQI GCTCTTC 1 cut(s) 467
BspT107I GGYRCC 2 cut(s) 101, 184
BsrDI GCAATG 1 cut(s) 221
BsrI ACTGG 1 cut(s) 609
BssECI CCNNGG 1 cut(s) 416
BssNI GRCGYC 1 cut(s) 102
Bst2UI CCWGG 3 cut(s) 269, 417, 436
Bst6I CTCTTC 2 cut(s) 48, 467
BstACI GRCGYC 1 cut(s) 102
BstC8I GCNNGC 1 cut(s) 499
BstDEI CTNAG 4 cut(s) 77, 311, 383, 527
BstENI CCTNNNNNAGG 2 cut(s) 314, 382
BstF5I GGATG 1 cut(s) 426
BstH2I RGCGCY 1 cut(s) 105
BstHHI GCGC 3 cut(s) 104, 232, 252
BstMAI GTCTC 1 cut(s) 74
BstMWI GCNNNNNNNGC 3 cut(s) 59, 227, 357
BstNI CCWGG 3 cut(s) 269, 417, 436
BstSCI CCNGG 3 cut(s) 267, 415, 434
BstV1I GCAGC 2 cut(s) 37, 40
BstV2I GAAGAC 1 cut(s) 277
BstXI CCANNNNNNTGG 1 cut(s) 181
BsuI GTATCC 2 cut(s) 339, 420
BsuRI GGCC 1 cut(s) 501
BtsCI GGATG 1 cut(s) 426
BtsI GCAGTG 2 cut(s) 94, 390
BtsIMutI CAGTG 2 cut(s) 94, 390
BveI ACCTGC 1 cut(s) 582
Cac8I GCNNGC 1 cut(s) 499
CfoI GCGC 3 cut(s) 104, 232, 252
Cfr13I GGNCC 1 cut(s) 265
Csp6I GTAC 3 cut(s) 241, 388, 445
CviAII CATG 2 cut(s) 57, 260
CviQI GTAC 3 cut(s) 241, 388, 445
DdeI CTNAG 4 cut(s) 77, 311, 383, 527
DinI GGCGCC 1 cut(s) 103
Eam1104I CTCTTC 2 cut(s) 48, 467
EarI CTCTTC 2 cut(s) 48, 467
Eco47I GGWCC 1 cut(s) 265
Eco57I CTGAAG 2 cut(s) 29, 493
EcoNI CCTNNNNNAGG 2 cut(s) 314, 382
EcoRII CCWGG 3 cut(s) 267, 415, 434
EgeI GGCGCC 1 cut(s) 103
EheI GGCGCC 1 cut(s) 103
FaeI CATG 2 cut(s) 60, 263
FatI CATG 2 cut(s) 56, 259
FauI CCCGC 1 cut(s) 502
FauNDI CATATG 1 cut(s) 579
Fnu4HI GCNGC 2 cut(s) 51, 54
FokI GGATG 1 cut(s) 433
Fsp4HI GCNGC 2 cut(s) 51, 54
GlaI GCGC 3 cut(s) 103, 231, 251
GluI GCNGC 2 cut(s) 51, 54
GsuI CTGGAG 1 cut(s) 399
HaeII RGCGCY 1 cut(s) 105
HaeIII GGCC 1 cut(s) 501
HhaI GCGC 3 cut(s) 104, 232, 252
Hin1I GRCGYC 1 cut(s) 102
Hin1II CATG 2 cut(s) 60, 263
Hin6I GCGC 3 cut(s) 102, 230, 250
HinP1I GCGC 3 cut(s) 102, 230, 250
HindIII AAGCTT 1 cut(s) 79
HinfI GANTC 1 cut(s) 272
HphI GGTGA 1 cut(s) 440
Hpy166II GTNNAC 1 cut(s) 390
Hpy188I TCNGA 2 cut(s) 210, 528
Hpy8I GTNNAC 1 cut(s) 390
HpyAV CCTTC 2 cut(s) 219, 517
HpyCH4IV ACGT 1 cut(s) 243
HpyCH4V TGCA 5 cut(s) 56, 214, 351, 395, 577
HpyF10VI GCNNNNNNNGC 3 cut(s) 59, 227, 357
HpyF3I CTNAG 4 cut(s) 77, 311, 383, 527
HpySE526I ACGT 1 cut(s) 243
Hsp92I GRCGYC 1 cut(s) 102
Hsp92II CATG 2 cut(s) 60, 263
HspAI GCGC 3 cut(s) 102, 230, 250
KasI GGCGCC 1 cut(s) 101
LguI GCTCTTC 1 cut(s) 467
LmnI GCTCC 4 cut(s) 141, 170, 306, 357
Lsp1109I GCAGC 2 cut(s) 37, 40
LweI GCATC 1 cut(s) 201
MaeII ACGT 1 cut(s) 243
MboII GAAGA 5 cut(s) 35, 277, 481, 484, 634
MluCI AATT 1 cut(s) 346
Mly113I GGCGCC 1 cut(s) 102
MlyI GAGTC 1 cut(s) 281
MnlI CCTC 8 cut(s) 216, 256, 310, 423, 488, 512, 522, 566
MseI TTAA 1 cut(s) 345
MspA1I CMGCKG 1 cut(s) 497
MspR9I CCNGG 3 cut(s) 269, 417, 436
Mva1269I GAATGC 1 cut(s) 395
MvaI CCWGG 3 cut(s) 269, 417, 436
MwoI GCNNNNNNNGC 3 cut(s) 59, 227, 357
NarI GGCGCC 1 cut(s) 102
NdeI CATATG 1 cut(s) 579
NlaIII CATG 2 cut(s) 60, 263
NlaIV GGNNCC 4 cut(s) 103, 172, 186, 308
NmeAIII GCCGAG 1 cut(s) 213
PciSI GCTCTTC 1 cut(s) 467
PctI GAATGC 1 cut(s) 395
PfoI TCCNGGA 1 cut(s) 267
PkrI GCNGC 2 cut(s) 52, 55
PleI GAGTC 1 cut(s) 280
PluTI GGCGCC 1 cut(s) 105
PpsI GAGTC 1 cut(s) 280
PsiI TTATAA 2 cut(s) 464, 519
Psp6I CCWGG 3 cut(s) 267, 415, 434
PspGI CCWGG 3 cut(s) 267, 415, 434
PspN4I GGNNCC 4 cut(s) 103, 172, 186, 308
PspPI GGNCC 1 cut(s) 265
RsaI GTAC 3 cut(s) 242, 389, 446
RsaNI GTAC 3 cut(s) 241, 388, 445
SapI GCTCTTC 1 cut(s) 467
SaqAI TTAA 1 cut(s) 345
SatI GCNGC 2 cut(s) 51, 54
Sau96I GGNCC 1 cut(s) 265
SchI GAGTC 1 cut(s) 281
ScrFI CCNGG 3 cut(s) 269, 417, 436
SfaNI GCATC 1 cut(s) 201
SfoI GGCGCC 1 cut(s) 103
SinI GGWCC 1 cut(s) 265
SmlI CTYRAG 1 cut(s) 82
SmoI CTYRAG 1 cut(s) 82
Sse9I AATT 1 cut(s) 346
SsiI CCGC 1 cut(s) 495
SspDI GGCGCC 1 cut(s) 101
StyD4I CCNGG 3 cut(s) 267, 415, 434
TaiI ACGT 1 cut(s) 246
TasI AATT 1 cut(s) 346
Tru1I TTAA 1 cut(s) 345
Tru9I TTAA 1 cut(s) 345
TscAI CASTG 2 cut(s) 94, 397
TseI GCWGC 2 cut(s) 50, 53
TspDTI ATGAA 2 cut(s) 277, 388
TspGWI ACGGA 1 cut(s) 183
TspRI CASTG 2 cut(s) 94, 397
VpaK11BI GGWCC 1 cut(s) 265
XagI CCTNNNNNAGG 2 cut(s) 314, 382
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.