AT5G62050

Mitochondrial inner membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0009287
Physical Location & Seq
Reverse (-)
24923465 .. 24927314
3850 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G62050.1

Sequence Viewer

Length: 1290 bp
ATGGCTTTCAGGCAAACTTTATCTATAAGGTCCAGGCTTTTCGCTCGTCGTAACCAACCAGTTTATCATATTATCCCTCGTGAGAGTGATCATGAAAGGGATTCATTCTGTCAAGAAACATCTCAGAGAAGTTACCATAGCTTTCTTCACCAAAGATCCGTTAACAATTCTGATTTCTCCAAAGTTTCTGGAGGAAGCTTACATTTGCCTCTTGCTCCCACGTCTGGTTTTGCTTTCTATCGTTATATGTCAAGTGCACCTGGTGTTGGTTCAGAGAAGATTGGTGTGATGTCTGACATTGCAGAAGTCATAACCGACTCAACATTGCAAGATGTGCCAGCTCAGGCTGCTGCTGCTGTCAGTGAAGTTACACTTGCAGCTGCTGACTCTTTCTTCCCTATCGCGGCCCTTCAGCAGTGCATTGATATGGTGCATACATTCACAGGCTTTGAGTGGTGGGCATCCATTGTTGTAGCAACCATTTTGATCCGATCATCAACAGTTCCTCTCTTGATCAAACAAATGAAAGACACAACCAAGTTAGCGCTGATGAGGCCACGGTTGGAGTCCATCCGGGAGGAAATGCAAAACAAGGGAATGGACTCGGTTACGATGGCAGAAGGTCAAAAAAAGATGAAGAATTTGTTTAAAGAATATGGTGTCACTCCATTCACCCCAATGAAAGGGATGTTTATTCAGGGACCGCTGTTCATCTGCTTTTTCCTTGCTATTCGAAATATGGCAGAGAAGGTACCTTCATTCCAAACAGGAGGTGCATTATGGTTTACCGATCTAACAACTCCCGACAGCTTATACATCTTGCCGGTTATAACAGGATTGACATTCTTGATAACCGTTGAGTGTAATGCACAAGAAGGCATGGAAGGGAATCCGATGGCTGGCACTGTAAAAACTGTTTGTCGGGTTTTTGCTCTCCTCACAGTCCCGATGACAATGAGTTTTCCACAGGCCATATTTTGTTACTGGATCACATCCAACCTGTTCTCTCTCATGTATGGACTTGTGATAAAGCGTCCTCAAGTGAAGAAGATGTTACGCATACCTGATCTGCCTCCACCTCCTCCAGGTCAACAACCTTCATTTGACCTGTTTTCAGCTCTCAAGAAAATGAAAGCCATGACACAGGACCATACACAGAACCAGATAGAACCACCTTCGCCAGTAAACCCGAGGCTTTCCTCAACATCGCTGAGTCCTGTTAGTAAGAGGCTCAAGGCTTTGGAGAGCCAAGTAAAGGGAAGGAAGAAGAATAGCAGCAAGAAGAAGTGA

Protein Analysis

429

Amino Acids

47.86

Weight (kDa)

9.55

Isoelectric Point (pI)

52.27

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
60KD_IMP PF02096 152 - 345 4e-28 60Kd inner membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AanI TTATAA 1 cut(s) 830
Acc65I GGTACC 1 cut(s) 751
AccB1I GGYRCC 1 cut(s) 751
AccII CGCG 1 cut(s) 404
AciI CCGC 2 cut(s) 404, 704
AclWI GGATC 3 cut(s) 150, 481, 995
AcsI RAATTY 1 cut(s) 640
AcuI CTGAAG 1 cut(s) 395
AdeI CACNNNGTG 1 cut(s) 263
AfaI GTAC 1 cut(s) 753
AfeI AGCGCT 1 cut(s) 546
AfiI CCNNNNNNNGG 7 cut(s) 224, 266, 403, 683, 899, 1085, 1255
AjiI CACGTC 1 cut(s) 222
AjnI CCWGG 3 cut(s) 32, 259, 1084
AluBI AGCT 6 cut(s) 141, 198, 341, 380, 810, 1118
AluI AGCT 6 cut(s) 141, 198, 341, 380, 810, 1118
Alw21I GWGCWC 1 cut(s) 259
Alw44I GTGCAC 1 cut(s) 255
AlwI GGATC 3 cut(s) 150, 481, 995
AlwNI CAGNNNCTG 1 cut(s) 383
Ama87I CYCGRG 1 cut(s) 1189
Aor51HI AGCGCT 1 cut(s) 546
AoxI GGCC 3 cut(s) 405, 554, 969
ApaLI GTGCAC 1 cut(s) 255
ApeKI GCWGC 6 cut(s) 347, 350, 353, 377, 380, 1275
ApoI RAATTY 1 cut(s) 640
Asp718I GGTACC 1 cut(s) 751
AspLEI GCGC 1 cut(s) 547
AspS9I GGNCC 4 cut(s) 30, 406, 701, 1147
AsuC2I CCSGG 1 cut(s) 575
AsuHPI GGTGA 2 cut(s) 140, 664
AsuII TTCGAA 1 cut(s) 733
AvaI CYCGRG 1 cut(s) 1189
AvaII GGWCC 3 cut(s) 30, 701, 1147
BaeGI GKGCMC 1 cut(s) 259
BanI GGYRCC 1 cut(s) 751
BauI CACGAG 1 cut(s) 78
Bbv12I GWGCWC 1 cut(s) 259
BbvI GCAGC 5 cut(s) 334, 337, 340, 367, 389
BccI CCATC 3 cut(s) 578, 607, 889
BciT130I CCWGG 3 cut(s) 34, 261, 1086
BclI TGATCA 2 cut(s) 88, 513
BcnI CCSGG 1 cut(s) 575
BfoI RGCGCY 1 cut(s) 548
BisI GCNGC 7 cut(s) 348, 351, 354, 378, 381, 405, 1276
BlsI GCNGC 7 cut(s) 349, 352, 355, 379, 382, 406, 1277
Bme1390I CCNGG 4 cut(s) 34, 261, 575, 1086
Bme18I GGWCC 3 cut(s) 30, 701, 1147
BmeT110I CYCGRG 1 cut(s) 1189
BmgBI CACGTC 1 cut(s) 222
BmgT120I GGNCC 4 cut(s) 30, 406, 701, 1147
BmiI GGNNCC 2 cut(s) 702, 753
BmrFI CCNGG 4 cut(s) 34, 261, 575, 1086
BmsI GCATC 1 cut(s) 470
BpmI CTGGAG 2 cut(s) 210, 1068
Bpu10I CCTNAGC 1 cut(s) 342
Bpu14I TTCGAA 1 cut(s) 733
BpuEI CTTGAG 3 cut(s) 1023, 1106, 1217
BpuMI CCSGG 1 cut(s) 575
BsaJI CCNNGG 2 cut(s) 557, 1190
Bsc4I CCNNNNNNNGG 7 cut(s) 224, 266, 403, 683, 899, 1085, 1255
Bse118I RCCGGY 1 cut(s) 823
Bse1I ACTGG 3 cut(s) 59, 989, 1181
Bse3DI GCAATG 2 cut(s) 297, 323
BseBI CCWGG 3 cut(s) 34, 261, 1086
BseDI CCNNGG 2 cut(s) 557, 1190
BseGI GGATG 4 cut(s) 461, 570, 693, 992
BseLI CCNNNNNNNGG 7 cut(s) 224, 266, 403, 683, 899, 1085, 1255
BseMI GCAATG 2 cut(s) 297, 323
BseMII CTCAG 3 cut(s) 137, 356, 1202
BseNI ACTGG 3 cut(s) 59, 989, 1181
BseRI GAGGAG 2 cut(s) 926, 1071
BseSI GKGCMC 1 cut(s) 259
BseXI GCAGC 5 cut(s) 334, 337, 340, 367, 389
Bsh1236I CGCG 1 cut(s) 404
BshFI GGCC 3 cut(s) 407, 556, 971
BshNI GGYRCC 1 cut(s) 751
BsiHKAI GWGCWC 1 cut(s) 259
BsiHKCI CYCGRG 1 cut(s) 1189
BsiSI CCGG 2 cut(s) 574, 824
BslFI GGGAC 2 cut(s) 714, 929
BslI CCNNNNNNNGG 7 cut(s) 224, 266, 403, 683, 899, 1085, 1255
BsmFI GGGAC 2 cut(s) 714, 929
BsnI GGCC 3 cut(s) 407, 556, 971
BsoBI CYCGRG 1 cut(s) 1189
Bsp119I TTCGAA 1 cut(s) 733
Bsp1286I GDGCHC 1 cut(s) 259
Bsp143I GATC 8 cut(s) 88, 155, 486, 491, 513, 790, 987, 1066
BspACI CCGC 2 cut(s) 404, 704
BspANI GGCC 3 cut(s) 407, 556, 971
BspCNI CTCAG 3 cut(s) 136, 355, 1203
BspFNI CGCG 1 cut(s) 404
BspHI TCATGA 1 cut(s) 91
BspLI GGNNCC 2 cut(s) 702, 753
BspPI GGATC 3 cut(s) 150, 481, 995
BspT104I TTCGAA 1 cut(s) 733
BspT107I GGYRCC 1 cut(s) 751
BsrDI GCAATG 2 cut(s) 297, 323
BsrFI RCCGGY 1 cut(s) 823
BsrI ACTGG 3 cut(s) 59, 989, 1181
BssAI RCCGGY 1 cut(s) 823
BssECI CCNNGG 2 cut(s) 557, 1190
BssMI GATC 8 cut(s) 88, 155, 486, 491, 513, 790, 987, 1066
BssSI CACGAG 1 cut(s) 78
Bst2BI CACGAG 1 cut(s) 78
Bst2UI CCWGG 3 cut(s) 34, 261, 1086
Bst4CI ACNGT 6 cut(s) 502, 561, 856, 907, 916, 943
BstAPI GCANNNNNTGC 1 cut(s) 334
BstBI TTCGAA 1 cut(s) 733
BstC8I GCNNGC 2 cut(s) 339, 901
BstDEI CTNAG 3 cut(s) 123, 342, 1211
BstDSI CCRYGG 1 cut(s) 557
BstENI CCTNNNNNAGG 1 cut(s) 1083
BstF5I GGATG 4 cut(s) 461, 570, 693, 992
BstFNI CGCG 1 cut(s) 404
BstH2I RGCGCY 1 cut(s) 548
BstHHI GCGC 1 cut(s) 547
BstKTI GATC 8 cut(s) 91, 158, 489, 494, 516, 793, 990, 1069
BstMBI GATC 8 cut(s) 88, 155, 486, 491, 513, 790, 987, 1066
BstMWI GCNNNNNNNGC 4 cut(s) 334, 347, 353, 553
BstNI CCWGG 3 cut(s) 34, 261, 1086
BstSCI CCNGG 4 cut(s) 32, 259, 573, 1084
BstSLI GKGCMC 1 cut(s) 259
BstUI CGCG 1 cut(s) 404
BstV1I GCAGC 5 cut(s) 334, 337, 340, 367, 389
BstX2I RGATCY 1 cut(s) 155
BstYI RGATCY 1 cut(s) 155
BsuRI GGCC 3 cut(s) 407, 556, 971
BtgI CCRYGG 1 cut(s) 557
BtgZI GCGATG 1 cut(s) 1191
BtrI CACGTC 1 cut(s) 222
BtsCI GGATG 4 cut(s) 461, 570, 693, 992
BtsI GCAGTG 1 cut(s) 422
BtsIMutI CAGTG 3 cut(s) 367, 422, 903
Cac8I GCNNGC 2 cut(s) 339, 901
CaiI CAGNNNCTG 1 cut(s) 383
CciI TCATGA 1 cut(s) 91
CfoI GCGC 1 cut(s) 547
Cfr10I RCCGGY 1 cut(s) 823
Cfr13I GGNCC 4 cut(s) 30, 406, 701, 1147
CseI GACGC 1 cut(s) 1022
CsiI ACCWGGT 1 cut(s) 259
Csp6I GTAC 1 cut(s) 752
CviAII CATG 4 cut(s) 92, 880, 1012, 1138
CviQI GTAC 1 cut(s) 752
DdeI CTNAG 3 cut(s) 123, 342, 1211
DpnI GATC 8 cut(s) 90, 157, 488, 493, 515, 792, 989, 1068
DpnII GATC 8 cut(s) 88, 155, 486, 491, 513, 790, 987, 1066
DraI TTTAAA 1 cut(s) 649
DraIII CACNNNGTG 1 cut(s) 263
Eco47I GGWCC 3 cut(s) 30, 701, 1147
Eco47III AGCGCT 1 cut(s) 546
Eco57I CTGAAG 1 cut(s) 395
Eco88I CYCGRG 1 cut(s) 1189
EcoNI CCTNNNNNAGG 1 cut(s) 1083
EcoRII CCWGG 3 cut(s) 32, 259, 1084
FaeI CATG 4 cut(s) 95, 883, 1015, 1141
FalI AAGNNNNNCTT 2 cut(s) 357, 389
FaqI GGGAC 2 cut(s) 714, 929
FatI CATG 4 cut(s) 91, 879, 1011, 1137
FbaI TGATCA 2 cut(s) 88, 513
Fnu4HI GCNGC 7 cut(s) 348, 351, 354, 378, 381, 405, 1276
FokI GGATG 4 cut(s) 448, 557, 700, 979
Fsp4HI GCNGC 7 cut(s) 348, 351, 354, 378, 381, 405, 1276
GlaI GCGC 1 cut(s) 546
GluI GCNGC 7 cut(s) 348, 351, 354, 378, 381, 405, 1276
GsuI CTGGAG 2 cut(s) 210, 1068
HaeII RGCGCY 1 cut(s) 548
HaeIII GGCC 3 cut(s) 407, 556, 971
HapII CCGG 2 cut(s) 574, 824
HgaI GACGC 1 cut(s) 1022
HhaI GCGC 1 cut(s) 547
Hin1II CATG 4 cut(s) 95, 883, 1015, 1141
Hin6I GCGC 1 cut(s) 545
HinP1I GCGC 1 cut(s) 545
HincII GTYRAC 2 cut(s) 163, 1091
HindII GTYRAC 2 cut(s) 163, 1091
HindIII AAGCTT 1 cut(s) 196
HinfI GANTC 7 cut(s) 101, 317, 386, 566, 602, 889, 1213
HpaI GTTAAC 1 cut(s) 163
HpaII CCGG 2 cut(s) 574, 824
HphI GGTGA 2 cut(s) 140, 664
Hpy166II GTNNAC 5 cut(s) 163, 257, 786, 1091, 1186
Hpy188I TCNGA 6 cut(s) 126, 172, 274, 295, 491, 894
Hpy188III TCNNGA 9 cut(s) 80, 92, 113, 189, 511, 803, 847, 946, 1123
Hpy8I GTNNAC 5 cut(s) 163, 257, 786, 1091, 1186
Hpy99I CGWCG 1 cut(s) 51
HpyAV CCTTC 9 cut(s) 419, 614, 742, 765, 869, 878, 1107, 1185, 1254
HpyCH4III ACNGT 6 cut(s) 502, 561, 856, 907, 916, 943
HpyCH4IV ACGT 1 cut(s) 221
HpyCH4V TGCA 9 cut(s) 257, 302, 328, 377, 420, 433, 586, 776, 869
HpyF10VI GCNNNNNNNGC 4 cut(s) 334, 347, 353, 553
HpyF3I CTNAG 3 cut(s) 123, 342, 1211
HpySE526I ACGT 1 cut(s) 221
Hsp92II CATG 4 cut(s) 95, 883, 1015, 1141
HspAI GCGC 1 cut(s) 545
KpnI GGTACC 1 cut(s) 755
Ksp22I TGATCA 2 cut(s) 88, 513
KspAI GTTAAC 1 cut(s) 163
Kzo9I GATC 8 cut(s) 88, 155, 486, 491, 513, 790, 987, 1066
LmnI GCTCC 1 cut(s) 220
Lsp1109I GCAGC 5 cut(s) 334, 337, 340, 367, 389
LweI GCATC 1 cut(s) 470
MabI ACCWGGT 1 cut(s) 259
MaeII ACGT 1 cut(s) 221
MaeIII GTNAC 7 cut(s) 50, 131, 367, 607, 661, 980, 1053
MalI GATC 8 cut(s) 90, 157, 488, 493, 515, 792, 989, 1068
MboI GATC 8 cut(s) 88, 155, 486, 491, 513, 790, 987, 1066
MboII GAAGA 8 cut(s) 137, 289, 385, 649, 1057, 1060, 1276, 1279
MflI RGATCY 1 cut(s) 155
MhlI GDGCHC 1 cut(s) 259
MluCI AATT 2 cut(s) 166, 640
MlyI GAGTC 5 cut(s) 311, 380, 575, 596, 1222
MmeI TCCRAC 2 cut(s) 543, 1020
MseI TTAA 2 cut(s) 162, 648
MslI CAYNNNNRTG 2 cut(s) 425, 677
MspA1I CMGCKG 2 cut(s) 380, 706
MspI CCGG 2 cut(s) 574, 824
MspR9I CCNGG 4 cut(s) 34, 261, 575, 1086
MvaI CCWGG 3 cut(s) 34, 261, 1086
MvnI CGCG 1 cut(s) 404
MwoI GCNNNNNNNGC 4 cut(s) 334, 347, 353, 553
NciI CCSGG 1 cut(s) 575
NdeII GATC 8 cut(s) 88, 155, 486, 491, 513, 790, 987, 1066
NlaIII CATG 4 cut(s) 95, 883, 1015, 1141
NlaIV GGNNCC 2 cut(s) 702, 753
NmuCI GTSAC 1 cut(s) 661
NspV TTCGAA 1 cut(s) 733
PagI TCATGA 1 cut(s) 91
PfeI GAWTC 2 cut(s) 101, 889
PfoI TCCNGGA 1 cut(s) 573
PkrI GCNGC 7 cut(s) 349, 352, 355, 379, 382, 406, 1277
PleI GAGTC 5 cut(s) 311, 380, 574, 596, 1221
PpsI GAGTC 5 cut(s) 311, 380, 574, 596, 1221
PsiI TTATAA 1 cut(s) 830
Psp6I CCWGG 3 cut(s) 32, 259, 1084
PspGI CCWGG 3 cut(s) 32, 259, 1084
PspN4I GGNNCC 2 cut(s) 702, 753
PspPI GGNCC 4 cut(s) 30, 406, 701, 1147
PstNI CAGNNNCTG 1 cut(s) 383
PsuI RGATCY 1 cut(s) 155
PvuII CAGCTG 1 cut(s) 380
RsaI GTAC 1 cut(s) 753
RsaNI GTAC 1 cut(s) 752
RseI CAYNNNNRTG 2 cut(s) 425, 677
SaqAI TTAA 2 cut(s) 162, 648
SatI GCNGC 7 cut(s) 348, 351, 354, 378, 381, 405, 1276
Sau3AI GATC 8 cut(s) 88, 155, 486, 491, 513, 790, 987, 1066
Sau96I GGNCC 4 cut(s) 30, 406, 701, 1147
SchI GAGTC 5 cut(s) 311, 380, 575, 596, 1222
ScrFI CCNGG 4 cut(s) 34, 261, 575, 1086
SduI GDGCHC 1 cut(s) 259
SexAI ACCWGGT 1 cut(s) 259
SfaNI GCATC 1 cut(s) 470
SfuI TTCGAA 1 cut(s) 733
SinI GGWCC 3 cut(s) 30, 701, 1147
SmiMI CAYNNNNRTG 2 cut(s) 425, 677
SmlI CTYRAG 3 cut(s) 1038, 1121, 1232
SmoI CTYRAG 3 cut(s) 1038, 1121, 1232
Sse9I AATT 2 cut(s) 166, 640
SsiI CCGC 2 cut(s) 404, 704
StyD4I CCNGG 4 cut(s) 32, 259, 573, 1084
TaaI ACNGT 6 cut(s) 502, 561, 856, 907, 916, 943
TaiI ACGT 1 cut(s) 224
TaqI TCGA 1 cut(s) 733
TasI AATT 2 cut(s) 166, 640
TauI GCSGC 1 cut(s) 407
TfiI GAWTC 2 cut(s) 101, 889
Tru1I TTAA 2 cut(s) 162, 648
Tru9I TTAA 2 cut(s) 162, 648
TscAI CASTG 3 cut(s) 367, 422, 910
TseFI GTSAC 1 cut(s) 661
TseI GCWGC 6 cut(s) 347, 350, 353, 377, 380, 1275
Tsp45I GTSAC 1 cut(s) 661
TspDTI ATGAA 9 cut(s) 93, 108, 539, 650, 695, 700, 747, 1089, 1145
TspGWI ACGGA 1 cut(s) 148
TspRI CASTG 3 cut(s) 367, 422, 910
VneI GTGCAC 1 cut(s) 255
VpaK11BI GGWCC 3 cut(s) 30, 701, 1147
XagI CCTNNNNNAGG 1 cut(s) 1083
XapI RAATTY 1 cut(s) 640
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.