Rroxscaffold_3G00260890

Mitochondrial inner membrane protein

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0009287
Physical Location & Seq
Reverse (-)
55209098 .. 55213820
4723 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00260890.1

Sequence Viewer

Length: 1314 bp
ATGGCTTACTGGCGAGGCCTCTCGTCCAGAGCGAGCATCATTGCTCGGCGAAAGCAGCACCATCCCTGCATCTCTCACATTCTCCACGACGACGACGTCGTTTCGGAACCCTCTCCTTCCCCAAATACCAATCATTTCTTCACCCAAAAGCGGTTCTTCAGTTTGGATAATAAAGTTTCAGGCTTTGGTGGGTTTGGTCAGAATCGGAGGATTTCAAATGCTCCGCTTTCGTCGCCGGCTGGATTCGGGTTTGCTCGGAATATGTCGACGGCGGGCGGGGGAGGGGCGGATAAGATTGGGGTGTTCAGTGATGTGCCGGAGATTCTGTCAGACACGACGGTGCAAGCTGTGACTTCGCAGCTGCCGGTGGTGAATGAGGTGGCGATTGCGGCGGCTGATTCCTACTTGCCGGTTAAGGGCTTGCAGTACTTGATTGATTATGTCCATACTACTGCTGGGTTGAGCTGGTGGGCTGCTATAGTGGTAACAACTCTTTTGATTCGAAGCTGCACGGTTCCGCTTCTAGTAAACCAACTTAAAGCAACGGCAAAACTCACTCTTATGAGGCCACGTCTAGAGGAACTGAAGCAAGAGATGGAAGAAAAGGGTATGGATCTCGGAGCTAAGATTGAATATCAAAAGAAAATTAAGATGCTGTTCAAGGAATATGGCTGTAGTCCCTTAACTCCACTGAAGGGACTCTTTATTCAAGCTCCTGTCTTCATCAGCTTTTTCCTAGCAATATCGAACATGGCAGAGAAGGTGCCATCATTTAAAGATGGTGGTGCCTATTGGTTTGTTGATCTGACAACTCCTGATCCTATGTACATTTTTCCAGTTTTGGCAGGATTGACATTCTTAATAACAGTGGAGAGTGAGTTTGCACAGTGCAATCTGCAAGAAGGTATGGAGGGAAATCCTGTTGCTAAAACCATGAAGAATGTTTCAAGAGGCATGGCTGTTCTTACAGTTCCATTTACCATGGGCTTTCCAAAGGCTGTATTTTGTTACTGGGTTACATCCAACTTGTTTTCGCTCGGATATGGAATTGTAATTAAGAATCCTTTTGTGAAGAAGACACTGGGTCTTCCTCAGCTGCCTACCCCTGCAACACAACCACAAGGATCACAACAATCTGCATTCTCACTGTTTTCACAAATGAAACAGCTGGCTGCAGCCCCAGAGCCCGCCTCATCAACCATTCAACCAGCAAAGATTCAAGACCGGAGAACATCATCGACATCAGATCTCAGTCAAAGAATTAAAAGTTTAGAGAAAAAAGTAAAGGGAAAAAAGAGAAACAAGAAGAGGTAG

Protein Analysis

437

Amino Acids

48.05

Weight (kDa)

9.63

Isoelectric Point (pI)

48.75

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
60KD_IMP PF02096 156 - 353 1.9e-27 60Kd inner membrane protein
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter
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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AasI GACNNNNNNGTC 1 cut(s) 95
AatII GACGTC 1 cut(s) 99
AccB1I GGYRCC 2 cut(s) 763, 785
AccI GTMKAC 1 cut(s) 266
AciI CCGC 9 cut(s) 151, 224, 272, 276, 287, 389, 392, 518, 1188
AclWI GGATC 3 cut(s) 621, 812, 1132
AcuI CTGAAG 3 cut(s) 142, 605, 713
AcyI GRCGYC 1 cut(s) 96
AfaI GTAC 2 cut(s) 428, 827
AfiI CCNNNNNNNGG 3 cut(s) 150, 416, 695
AgsI TTSAA 7 cut(s) 216, 632, 661, 710, 948, 1205, 1220
AhdI GACNNNNNGTC 1 cut(s) 1083
AjiI CACGTC 1 cut(s) 572
AjuI GAANNNNNNNTTGG 2 cut(s) 122, 154
AleI CACNNNNGTG 1 cut(s) 338
AluBI AGCT 9 cut(s) 347, 361, 465, 507, 623, 713, 729, 1096, 1168
AluI AGCT 9 cut(s) 347, 361, 465, 507, 623, 713, 729, 1096, 1168
AlwI GGATC 3 cut(s) 621, 812, 1132
AoxI GGCC 2 cut(s) 16, 566
ApeKI GCWGC 8 cut(s) 55, 358, 361, 473, 507, 1096, 1172, 1175
AsuHPI GGTGA 2 cut(s) 133, 382
AsuII TTCGAA 1 cut(s) 502
BanI GGYRCC 2 cut(s) 763, 785
BanII GRGCYC 1 cut(s) 1188
BbsI GAAGAC 3 cut(s) 712, 1079, 1082
BbvCI CCTCAGC 1 cut(s) 1092
BbvI GCAGC 8 cut(s) 67, 348, 370, 460, 494, 1083, 1159, 1187
BccI CCATC 4 cut(s) 69, 589, 773, 775
BceAI ACGGC 2 cut(s) 285, 561
BfaI CTAG 3 cut(s) 524, 575, 737
BfmI CTRYAG 3 cut(s) 477, 673, 1173
BglII AGATCT 1 cut(s) 1246
BmcAI AGTACT 1 cut(s) 428
BmeRI GACNNNNNGTC 1 cut(s) 1083
BmgBI CACGTC 1 cut(s) 572
BmiI GGNNCC 4 cut(s) 108, 516, 765, 787
BmrI ACTGGG 2 cut(s) 1021, 1091
BmsI GCATC 3 cut(s) 45, 78, 642
BmuI ACTGGG 2 cut(s) 1021, 1091
BpiI GAAGAC 3 cut(s) 712, 1079, 1082
BplI GAGNNNNNCTC 2 cut(s) 1175, 1207
Bpu10I CCTNAGC 1 cut(s) 1092
Bpu14I TTCGAA 1 cut(s) 502
BsaHI GRCGYC 1 cut(s) 96
BsaJI CCNNGG 1 cut(s) 981
BsaWI WCCGGW 1 cut(s) 1224
BsaXI ACNNNNNCTCC 4 cut(s) 311, 341, 863, 893
Bsc4I CCNNNNNNNGG 3 cut(s) 150, 416, 695
Bse118I RCCGGY 3 cut(s) 235, 364, 409
Bse1I ACTGG 4 cut(s) 14, 836, 1016, 1086
Bse3DI GCAATG 1 cut(s) 39
BseDI CCNNGG 1 cut(s) 981
BseGI GGATG 2 cut(s) 61, 1019
BseLI CCNNNNNNNGG 3 cut(s) 150, 416, 695
BseMI GCAATG 1 cut(s) 39
BseMII CTCAG 2 cut(s) 1106, 1264
BseNI ACTGG 4 cut(s) 14, 836, 1016, 1086
BseXI GCAGC 8 cut(s) 67, 348, 370, 460, 494, 1083, 1159, 1187
BseYI CCCAGC 1 cut(s) 455
BsgI GTGCAG 1 cut(s) 493
BshFI GGCC 2 cut(s) 18, 568
BshNI GGYRCC 2 cut(s) 763, 785
BsiSI CCGG 5 cut(s) 236, 317, 365, 410, 1225
BslFI GGGAC 2 cut(s) 663, 711
BslI CCNNNNNNNGG 3 cut(s) 150, 416, 695
BsmFI GGGAC 2 cut(s) 663, 711
BsmI GAATGC 1 cut(s) 1139
BsnI GGCC 2 cut(s) 18, 568
Bsp119I TTCGAA 1 cut(s) 502
Bsp1286I GDGCHC 1 cut(s) 1188
Bsp1407I TGTACA 1 cut(s) 825
Bsp143I GATC 5 cut(s) 613, 802, 817, 1124, 1246
Bsp19I CCATGG 1 cut(s) 981
BspACI CCGC 9 cut(s) 151, 224, 272, 276, 287, 389, 392, 518, 1188
BspANI GGCC 2 cut(s) 18, 568
BspCNI CTCAG 2 cut(s) 1105, 1263
BspLI GGNNCC 4 cut(s) 108, 516, 765, 787
BspMAI CTGCAG 1 cut(s) 1177
BspPI GGATC 3 cut(s) 621, 812, 1132
BspT104I TTCGAA 1 cut(s) 502
BspT107I GGYRCC 2 cut(s) 763, 785
BsrDI GCAATG 1 cut(s) 39
BsrFI RCCGGY 3 cut(s) 235, 364, 409
BsrGI TGTACA 1 cut(s) 825
BsrI ACTGG 4 cut(s) 14, 836, 1016, 1086
BssAI RCCGGY 3 cut(s) 235, 364, 409
BssECI CCNNGG 1 cut(s) 981
BssMI GATC 5 cut(s) 613, 802, 817, 1124, 1246
BssNI GRCGYC 1 cut(s) 96
BssT1I CCWWGG 1 cut(s) 981
Bst4CI ACNGT 6 cut(s) 340, 514, 868, 888, 970, 1149
Bst6I CTCTTC 1 cut(s) 1301
BstACI GRCGYC 1 cut(s) 96
BstAUI TGTACA 1 cut(s) 825
BstBI TTCGAA 1 cut(s) 502
BstC8I GCNNGC 7 cut(s) 34, 237, 274, 345, 422, 1170, 1188
BstDEI CTNAG 3 cut(s) 624, 1092, 1250
BstDSI CCRYGG 1 cut(s) 981
BstF5I GGATG 2 cut(s) 61, 1019
BstKTI GATC 5 cut(s) 616, 805, 820, 1127, 1249
BstMBI GATC 5 cut(s) 613, 802, 817, 1124, 1246
BstMWI GCNNNNNNNGC 3 cut(s) 55, 232, 389
BstSFI CTRYAG 3 cut(s) 477, 673, 1173
BstV1I GCAGC 8 cut(s) 67, 348, 370, 460, 494, 1083, 1159, 1187
BstV2I GAAGAC 3 cut(s) 712, 1079, 1082
BstX2I RGATCY 2 cut(s) 613, 1246
BstYI RGATCY 2 cut(s) 613, 1246
BsuRI GGCC 2 cut(s) 18, 568
BtgI CCRYGG 1 cut(s) 981
BtrI CACGTC 1 cut(s) 572
BtsCI GGATG 2 cut(s) 61, 1019
BtsIMutI CAGTG 6 cut(s) 313, 689, 873, 893, 1079, 1145
Cac8I GCNNGC 7 cut(s) 34, 237, 274, 345, 422, 1170, 1188
Cfr10I RCCGGY 3 cut(s) 235, 364, 409
Csp6I GTAC 2 cut(s) 427, 826
CviAII CATG 4 cut(s) 751, 934, 955, 982
CviQI GTAC 2 cut(s) 427, 826
DdeI CTNAG 3 cut(s) 624, 1092, 1250
DpnI GATC 5 cut(s) 615, 804, 819, 1126, 1248
DpnII GATC 5 cut(s) 613, 802, 817, 1124, 1246
DraI TTTAAA 1 cut(s) 775
DrdI GACNNNNNNGTC 1 cut(s) 95
DriI GACNNNNNGTC 1 cut(s) 1083
DseDI GACNNNNNNGTC 1 cut(s) 95
Eam1104I CTCTTC 1 cut(s) 1301
Eam1105I GACNNNNNGTC 1 cut(s) 1083
EarI CTCTTC 1 cut(s) 1301
EciI GGCGGA 1 cut(s) 302
Eco130I CCWWGG 1 cut(s) 981
Eco147I AGGCCT 1 cut(s) 18
Eco24I GRGCYC 1 cut(s) 1188
Eco57I CTGAAG 3 cut(s) 142, 605, 713
EcoT14I CCWWGG 1 cut(s) 981
EcoT38I GRGCYC 1 cut(s) 1188
ErhI CCWWGG 1 cut(s) 981
FaeI CATG 4 cut(s) 754, 937, 958, 985
FalI AAGNNNNNCTT 4 cut(s) 140, 172, 686, 718
FaqI GGGAC 2 cut(s) 663, 711
FatI CATG 4 cut(s) 750, 933, 954, 981
FauI CCCGC 3 cut(s) 265, 269, 1195
FblI GTMKAC 1 cut(s) 266
FokI GGATG 2 cut(s) 48, 1006
FriOI GRGCYC 1 cut(s) 1188
FspBI CTAG 3 cut(s) 524, 575, 737
GsaI CCCAGC 1 cut(s) 459
HaeIII GGCC 2 cut(s) 18, 568
HapII CCGG 5 cut(s) 236, 317, 365, 410, 1225
Hin1I GRCGYC 1 cut(s) 96
Hin1II CATG 4 cut(s) 754, 937, 958, 985
HincII GTYRAC 1 cut(s) 267
HindII GTYRAC 1 cut(s) 267
HinfI GANTC 8 cut(s) 202, 243, 322, 398, 499, 699, 1060, 1216
HpaII CCGG 5 cut(s) 236, 317, 365, 410, 1225
HphI GGTGA 2 cut(s) 133, 382
Hpy166II GTNNAC 2 cut(s) 267, 529
Hpy188I TCNGA 9 cut(s) 106, 201, 207, 258, 331, 620, 807, 1040, 1246
Hpy188III TCNNGA 5 cut(s) 27, 575, 815, 948, 1220
Hpy8I GTNNAC 2 cut(s) 267, 529
Hpy99I CGWCG 7 cut(s) 92, 95, 98, 101, 235, 271, 340
HpyAV CCTTC 4 cut(s) 126, 688, 754, 896
HpyCH4III ACNGT 6 cut(s) 340, 514, 868, 888, 970, 1149
HpyCH4IV ACGT 2 cut(s) 96, 571
HpyF10VI GCNNNNNNNGC 3 cut(s) 55, 232, 389
HpyF3I CTNAG 3 cut(s) 624, 1092, 1250
HpySE526I ACGT 2 cut(s) 96, 571
Hsp92I GRCGYC 1 cut(s) 96
Hsp92II CATG 4 cut(s) 754, 937, 958, 985
KroI GCCGGC 1 cut(s) 235
KroNI GCCGGC 1 cut(s) 237
Kzo9I GATC 5 cut(s) 613, 802, 817, 1124, 1246
LmnI GCTCC 3 cut(s) 226, 620, 718
Lsp1109I GCAGC 8 cut(s) 67, 348, 370, 460, 494, 1083, 1159, 1187
LweI GCATC 3 cut(s) 45, 78, 642
MaeI CTAG 3 cut(s) 524, 575, 737
MaeII ACGT 2 cut(s) 96, 571
MaeIII GTNAC 4 cut(s) 349, 484, 1007, 1015
MalI GATC 5 cut(s) 615, 804, 819, 1126, 1248
MboI GATC 5 cut(s) 613, 802, 817, 1124, 1246
MboII GAAGA 8 cut(s) 130, 148, 611, 712, 949, 1079, 1084, 1087
MflI RGATCY 2 cut(s) 613, 1246
MhlI GDGCHC 1 cut(s) 1188
MluCI AATT 4 cut(s) 645, 1047, 1053, 1260
MlyI GAGTC 1 cut(s) 693
MmeI TCCRAC 1 cut(s) 1047
MroNI GCCGGC 1 cut(s) 235
MseI TTAA 8 cut(s) 414, 537, 648, 683, 774, 860, 1056, 1263
MslI CAYNNNNRTG 2 cut(s) 338, 560
MspA1I CMGCKG 3 cut(s) 361, 1096, 1168
MspI CCGG 5 cut(s) 236, 317, 365, 410, 1225
Mva1269I GAATGC 1 cut(s) 1139
MwoI GCNNNNNNNGC 3 cut(s) 55, 232, 389
NaeI GCCGGC 1 cut(s) 237
NcoI CCATGG 1 cut(s) 981
NdeII GATC 5 cut(s) 613, 802, 817, 1124, 1246
NgoMIV GCCGGC 1 cut(s) 235
NlaIII CATG 4 cut(s) 754, 937, 958, 985
NlaIV GGNNCC 4 cut(s) 108, 516, 765, 787
NmeAIII GCCGAG 1 cut(s) 25
NmuCI GTSAC 1 cut(s) 349
NspV TTCGAA 1 cut(s) 502
OliI CACNNNNGTG 1 cut(s) 338
PceI AGGCCT 1 cut(s) 18
PcsI WCGNNNNNNNCGW 3 cut(s) 29, 93, 96
PctI GAATGC 1 cut(s) 1139
PdiI GCCGGC 1 cut(s) 237
PfeI GAWTC 7 cut(s) 202, 243, 322, 398, 499, 1060, 1216
PflFI GACNNNGTC 1 cut(s) 95
PleI GAGTC 1 cut(s) 693
PpsI GAGTC 1 cut(s) 693
PspFI CCCAGC 1 cut(s) 455
PspN4I GGNNCC 4 cut(s) 108, 516, 765, 787
PstI CTGCAG 1 cut(s) 1177
PsuI RGATCY 2 cut(s) 613, 1246
PsyI GACNNNGTC 1 cut(s) 95
PvuII CAGCTG 3 cut(s) 361, 1096, 1168
RsaI GTAC 2 cut(s) 428, 827
RsaNI GTAC 2 cut(s) 427, 826
RseI CAYNNNNRTG 2 cut(s) 338, 560
SalI GTCGAC 1 cut(s) 265
SaqAI TTAA 8 cut(s) 414, 537, 648, 683, 774, 860, 1056, 1263
Sau3AI GATC 5 cut(s) 613, 802, 817, 1124, 1246
ScaI AGTACT 1 cut(s) 428
SchI GAGTC 1 cut(s) 693
SduI GDGCHC 1 cut(s) 1188
SfaNI GCATC 3 cut(s) 45, 78, 642
SfcI CTRYAG 3 cut(s) 477, 673, 1173
SfuI TTCGAA 1 cut(s) 502
SmiMI CAYNNNNRTG 2 cut(s) 338, 560
Sse9I AATT 4 cut(s) 645, 1047, 1053, 1260
SseBI AGGCCT 1 cut(s) 18
SsiI CCGC 9 cut(s) 151, 224, 272, 276, 287, 389, 392, 518, 1188
SspMI CTAG 3 cut(s) 524, 575, 737
StuI AGGCCT 1 cut(s) 18
StyI CCWWGG 1 cut(s) 981
TaaI ACNGT 6 cut(s) 340, 514, 868, 888, 970, 1149
TaiI ACGT 2 cut(s) 99, 574
TaqI TCGA 4 cut(s) 266, 502, 746, 1238
TasI AATT 4 cut(s) 645, 1047, 1053, 1260
TatI WGTACW 2 cut(s) 426, 825
TauI GCSGC 2 cut(s) 392, 395
TfiI GAWTC 7 cut(s) 202, 243, 322, 398, 499, 1060, 1216
Tru1I TTAA 8 cut(s) 414, 537, 648, 683, 774, 860, 1056, 1263
Tru9I TTAA 8 cut(s) 414, 537, 648, 683, 774, 860, 1056, 1263
TscAI CASTG 6 cut(s) 313, 696, 873, 893, 1086, 1152
TseFI GTSAC 1 cut(s) 349
TseI GCWGC 8 cut(s) 55, 358, 361, 473, 507, 1096, 1172, 1175
Tsp45I GTSAC 1 cut(s) 349
TspDTI ATGAA 3 cut(s) 712, 950, 1175
TspRI CASTG 6 cut(s) 313, 696, 873, 893, 1086, 1152
Tth111I GACNNNGTC 1 cut(s) 95
XbaI TCTAGA 1 cut(s) 574
XcmI CCANNNNNNNNNTGG 1 cut(s) 452
XmiI GTMKAC 1 cut(s) 266
XspI CTAG 3 cut(s) 524, 575, 737
ZraI GACGTC 1 cut(s) 97
ZrmI AGTACT 1 cut(s) 428
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.