AT5G62570

Calmodulin binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
arabidopsis_thaliana
5
OG0004999
Physical Location & Seq
Forward (+)
25113948 .. 25117314
3367 bp
Loading structure...
UTR
Exon/CDS
Intron
AT5G62570.2

Sequence Viewer

Length: 1530 bp
ATGTCACAGAAGAGAGAGTTTGAAGAAGGGAAAGGTCGAATTGAGGGCGGCTCATCCGAAGACAAGCGTAGAAGATTCAAAAGTGTGGTTCAGGAGGTAATGAGATTACAAACCGTCAAACATTTTCTCGAACCTGTTCTCGAGCCCTTAATTCGTAAAGTGGTCAAAGAAGAAGTTGAATTAGCTCTTGGAAAGCATCTAGCAGGCATCAAGTGGATTTGTGAGAAAGAGACTCATCCTTTGGAATCAAGGAATCTACAGTTAAAGTTCTTGAACAATCTATCGCTTCCCGTGTTTACTTCAGCTCGGATAGAAGGAGATGAAGGCCAAGCTATAAGAGTTGGATTAATAGACCCTTCAACTGGCCAAATTTTCTCTTCTGGTCCTGCATCATCCGCCAAACTCGAAGTTTTTGTCGTTGAGGGTGATTTTAACAGTGTTAGTGATTGGACTGATGAGGATATCAGGAATAACATTGTAAGAGAGAGAGAAGGAAAGAAACCTCTACTCAATGGGAATGTATTTGCGGTTCTTAATGATGGCATTGGTGTTATGGATGAAATCTCATTTACAGATAACTCTAGCTGGACTAGGAGCAGGAAGTTCAGACTCGGGGTACGAATAGTGGATCAGTTTGATTATGTCAAAATTAGAGAGGCAATAACAGAGTCCTTTGTCGTAAGAGATCATCGAGGAGAGTTGTACAAAAAGCATCATCCTCCTTCTCTTTTCGATGAAGTATGGAGATTGGAAAAGATAGGGAAAGATGGAGCATTTCACAGACGACTGAATTTGTCAAATATAAACACTGTCAAGGATTTCTTAACACATTTCCATTTAAACTCTTCAAAACTCCGACAAGTTCTTGGTACGGGTATGTCTTCAAAGATGTGGGAAATTACTTTGGACCATGCTCGATCATGTGTTCTAGATAGTAGCGTCCATGTGTACCAAGCTCCTGGATTTCAGAAGAAAACAGCTGTTGTCTTCAACGTCGTAGCTCAAGTGCTTGGACTGCTCGTGGATTTTCAGTATATTCCTGCTGAAAAGTTATCAGAGATTGAAAAGGCTCAAGCGGAGGTGATGGTAATTGATGCATTGAGTCACCTAAATGAAGTAATCTCGTATGATGATGAGGTTTCCATGATGAGGAACGTTCTCAATGCCCCGGCTTCTCAAGGCAGTGTTGCCGGGATTGATTATTCGGGTCTGAGTTTGACCAGTTTGGACGGATACGGTTTTGTGTCAAGTCTTCACAACACAGCAGAATGTAGTGGCAAGCACAGTGATGATGTTGACATGGAAGTTACTCCACATGGTTTATATGAAGACTACGACAATCTCTGGAACTGCTCTCACATTCTAGGCTTAGAAGAACCGCAATCTGAGTTGCAGAGTGCGCTTGACGATTTCATGTCACAGAAGAATGCTTCGGTCGGTGGTAAGGCTCACAGCAAAAGATGGACAAAGCTTTTCAGTGTCTCGAGATGGCTCTCTGTTTTCAAGTATGTCAAGTTAGGAAAAATATAA

Protein Analysis

509

Amino Acids

57.39

Weight (kDa)

6.13

Isoelectric Point (pI)

43.62

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 71 - 215 2.2e-55 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 228 - 293 7.7e-30 Calmodulin binding protein central domain
Calmod_bind_C PF20452 299 - 361 4.2e-14 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AciI CCGC 5 cut(s) 48, 396, 527, 1076, 1379
AclI AACGTT 1 cut(s) 1155
AclWI GGATC 1 cut(s) 636
AcoI YGGCCR 1 cut(s) 364
AcsI RAATTY 2 cut(s) 369, 790
AcuI CTGAAG 1 cut(s) 285
AfaI GTAC 4 cut(s) 618, 704, 871, 950
AfiI CCNNNNNNNGG 2 cut(s) 362, 1149
AjnI CCWGG 1 cut(s) 958
AjuI GAANNNNNNNTTGG 2 cut(s) 171, 203
AluBI AGCT 8 cut(s) 185, 305, 332, 585, 956, 980, 1001, 1471
AluI AGCT 8 cut(s) 185, 305, 332, 585, 956, 980, 1001, 1471
Alw26I GTCTC 2 cut(s) 224, 1486
AlwI GGATC 1 cut(s) 636
Ama87I CYCGRG 3 cut(s) 140, 611, 1483
AoxI GGCC 2 cut(s) 325, 364
ApoI RAATTY 2 cut(s) 369, 790
ArsI GACNNNNNNTTYG 4 cut(s) 223, 255, 399, 431
AseI ATTAAT 1 cut(s) 347
Asp700I GAANNNNTTC 1 cut(s) 135
AspLEI GCGC 1 cut(s) 1402
AspS9I GGNCC 2 cut(s) 383, 907
AsuC2I CCSGG 2 cut(s) 1169, 1192
AsuHPI GGTGA 3 cut(s) 437, 1093, 1097
AvaI CYCGRG 3 cut(s) 140, 611, 1483
AvaII GGWCC 2 cut(s) 383, 907
BalI TGGCCA 1 cut(s) 366
BanII GRGCYC 1 cut(s) 147
BauI CACGAG 1 cut(s) 1019
BbsI GAAGAC 5 cut(s) 66, 873, 979, 1244, 1335
BccI CCATC 5 cut(s) 533, 761, 1078, 1455, 1482
BciT130I CCWGG 1 cut(s) 960
BciVI GTATCC 1 cut(s) 1226
BcnI CCSGG 2 cut(s) 1169, 1192
BcoDI GTCTC 2 cut(s) 224, 1486
BfaI CTAG 5 cut(s) 200, 582, 591, 929, 1364
BfmI CTRYAG 1 cut(s) 257
BfuI GTATCC 1 cut(s) 1226
BisI GCNGC 1 cut(s) 49
BlsI GCNGC 1 cut(s) 50
Bme1390I CCNGG 3 cut(s) 960, 1169, 1192
Bme18I GGWCC 2 cut(s) 383, 907
BmeT110I CYCGRG 3 cut(s) 140, 611, 1483
BmgT120I GGNCC 2 cut(s) 383, 907
BmrFI CCNGG 3 cut(s) 960, 1169, 1192
BmsI GCATC 5 cut(s) 205, 216, 398, 721, 1084
BpiI GAAGAC 5 cut(s) 66, 873, 979, 1244, 1335
BplI GAGNNNNNCTC 2 cut(s) 35, 67
BpuEI CTTGAG 3 cut(s) 987, 1056, 1161
BpuMI CCSGG 2 cut(s) 1169, 1192
BsaJI CCNNGG 1 cut(s) 1167
Bsc4I CCNNNNNNNGG 2 cut(s) 362, 1149
Bse1I ACTGG 2 cut(s) 367, 1221
BseBI CCWGG 1 cut(s) 960
BseDI CCNNGG 1 cut(s) 1167
BseGI GGATG 5 cut(s) 53, 235, 392, 562, 715
BseLI CCNNNNNNNGG 2 cut(s) 362, 1149
BseMII CTCAG 2 cut(s) 1202, 1377
BseNI ACTGG 2 cut(s) 367, 1221
BseRI GAGGAG 1 cut(s) 708
Bsh1285I CGRYCG 1 cut(s) 1437
BshFI GGCC 2 cut(s) 327, 366
BsiEI CGRYCG 1 cut(s) 1437
BsiHKCI CYCGRG 3 cut(s) 140, 611, 1483
BsiSI CCGG 2 cut(s) 1169, 1191
BslI CCNNNNNNNGG 2 cut(s) 362, 1149
BsmAI GTCTC 2 cut(s) 224, 1486
BsmI GAATGC 1 cut(s) 1432
BsnI GGCC 2 cut(s) 327, 366
BsoBI CYCGRG 3 cut(s) 140, 611, 1483
Bsp1286I GDGCHC 1 cut(s) 147
Bsp1407I TGTACA 1 cut(s) 702
Bsp143I GATC 3 cut(s) 628, 685, 917
BspACI CCGC 5 cut(s) 48, 396, 527, 1076, 1379
BspANI GGCC 2 cut(s) 327, 366
BspCNI CTCAG 2 cut(s) 1203, 1378
BspPI GGATC 1 cut(s) 636
BsrGI TGTACA 1 cut(s) 702
BsrI ACTGG 2 cut(s) 367, 1221
BssECI CCNNGG 1 cut(s) 1167
BssMI GATC 3 cut(s) 628, 685, 917
BssSI CACGAG 1 cut(s) 1019
Bst2BI CACGAG 1 cut(s) 1019
Bst2UI CCWGG 1 cut(s) 960
Bst4CI ACNGT 6 cut(s) 115, 261, 437, 811, 1238, 1286
Bst6I CTCTTC 3 cut(s) 5, 382, 850
BstAUI TGTACA 1 cut(s) 702
BstC8I GCNNGC 2 cut(s) 205, 1280
BstDEI CTNAG 3 cut(s) 1211, 1369, 1386
BstF5I GGATG 5 cut(s) 53, 235, 392, 562, 715
BstHHI GCGC 1 cut(s) 1402
BstKTI GATC 3 cut(s) 631, 688, 920
BstMAI GTCTC 2 cut(s) 224, 1486
BstMBI GATC 3 cut(s) 628, 685, 917
BstMCI CGRYCG 1 cut(s) 1437
BstMWI GCNNNNNNNGC 3 cut(s) 395, 1015, 1399
BstNI CCWGG 1 cut(s) 960
BstSCI CCNGG 3 cut(s) 958, 1167, 1190
BstSFI CTRYAG 1 cut(s) 257
BstV2I GAAGAC 5 cut(s) 66, 873, 979, 1244, 1335
BstXI CCANNNNNNTGG 1 cut(s) 959
BsuI GTATCC 1 cut(s) 1226
BsuRI GGCC 2 cut(s) 327, 366
BtsCI GGATG 5 cut(s) 53, 235, 392, 562, 715
BtsI GCAGTG 1 cut(s) 1189
BtsIMutI CAGTG 5 cut(s) 442, 807, 1189, 1291, 1483
Cac8I GCNNGC 2 cut(s) 205, 1280
CfoI GCGC 1 cut(s) 1402
Cfr13I GGNCC 2 cut(s) 383, 907
CseI GACGC 1 cut(s) 928
Csp6I GTAC 4 cut(s) 617, 703, 870, 949
CviAII CATG 7 cut(s) 911, 921, 944, 1144, 1300, 1316, 1414
CviQI GTAC 4 cut(s) 617, 703, 870, 949
DdeI CTNAG 3 cut(s) 1211, 1369, 1386
DpnI GATC 3 cut(s) 630, 687, 919
DpnII GATC 3 cut(s) 628, 685, 917
DraI TTTAAA 1 cut(s) 840
EaeI YGGCCR 1 cut(s) 364
Eam1104I CTCTTC 3 cut(s) 5, 382, 850
EarI CTCTTC 3 cut(s) 5, 382, 850
EciI GGCGGA 1 cut(s) 385
Eco24I GRGCYC 1 cut(s) 147
Eco32I GATATC 1 cut(s) 463
Eco47I GGWCC 2 cut(s) 383, 907
Eco57I CTGAAG 1 cut(s) 285
Eco88I CYCGRG 3 cut(s) 140, 611, 1483
EcoRII CCWGG 1 cut(s) 958
EcoRV GATATC 1 cut(s) 463
EcoT22I ATGCAT 1 cut(s) 1099
EcoT38I GRGCYC 1 cut(s) 147
FaeI CATG 7 cut(s) 914, 924, 947, 1147, 1303, 1319, 1417
FalI AAGNNNNNCTT 2 cut(s) 806, 838
FatI CATG 7 cut(s) 910, 920, 943, 1143, 1299, 1315, 1413
Fnu4HI GCNGC 1 cut(s) 49
FokI GGATG 5 cut(s) 40, 222, 379, 569, 702
FriOI GRGCYC 1 cut(s) 147
Fsp4HI GCNGC 1 cut(s) 49
FspBI CTAG 5 cut(s) 200, 582, 591, 929, 1364
GlaI GCGC 1 cut(s) 1401
GluI GCNGC 1 cut(s) 49
HaeIII GGCC 2 cut(s) 327, 366
HapII CCGG 2 cut(s) 1169, 1191
HgaI GACGC 1 cut(s) 928
HhaI GCGC 1 cut(s) 1402
Hin1II CATG 7 cut(s) 914, 924, 947, 1147, 1303, 1319, 1417
Hin6I GCGC 1 cut(s) 1400
HinP1I GCGC 1 cut(s) 1400
HincII GTYRAC 1 cut(s) 1297
HindII GTYRAC 1 cut(s) 1297
HindIII AAGCTT 1 cut(s) 1469
HinfI GANTC 7 cut(s) 75, 232, 245, 253, 609, 668, 1102
HpaII CCGG 2 cut(s) 1169, 1191
HphI GGTGA 3 cut(s) 437, 1093, 1097
Hpy166II GTNNAC 3 cut(s) 297, 949, 1297
Hpy188I TCNGA 8 cut(s) 58, 309, 608, 857, 969, 1057, 1212, 1387
Hpy188III TCNNGA 9 cut(s) 92, 128, 140, 271, 466, 929, 1345, 1483, 1485
Hpy8I GTNNAC 3 cut(s) 297, 949, 1297
Hpy99I CGWCG 1 cut(s) 998
HpyAV CCTTC 6 cut(s) 20, 308, 317, 366, 485, 732
HpyCH4III ACNGT 6 cut(s) 115, 261, 437, 811, 1238, 1286
HpyCH4IV ACGT 2 cut(s) 993, 1155
HpyCH4V TGCA 3 cut(s) 389, 1097, 1393
HpyF10VI GCNNNNNNNGC 3 cut(s) 395, 1015, 1399
HpyF3I CTNAG 3 cut(s) 1211, 1369, 1386
HpySE526I ACGT 2 cut(s) 993, 1155
Hsp92II CATG 7 cut(s) 914, 924, 947, 1147, 1303, 1319, 1417
HspAI GCGC 1 cut(s) 1400
Kzo9I GATC 3 cut(s) 628, 685, 917
LmnI GCTCC 3 cut(s) 594, 770, 961
LweI GCATC 5 cut(s) 205, 216, 398, 721, 1084
MaeI CTAG 5 cut(s) 200, 582, 591, 929, 1364
MaeII ACGT 2 cut(s) 993, 1155
MaeIII GTNAC 4 cut(s) 3, 1103, 1306, 1416
MalI GATC 3 cut(s) 630, 687, 919
MboI GATC 3 cut(s) 628, 685, 917
MhlI GDGCHC 1 cut(s) 147
MlsI TGGCCA 1 cut(s) 366
MluCI AATT 8 cut(s) 39, 150, 179, 369, 648, 790, 897, 1089
MluNI TGGCCA 1 cut(s) 366
MlyI GAGTC 4 cut(s) 226, 603, 677, 1111
MmeI TCCRAC 2 cut(s) 322, 880
Mox20I TGGCCA 1 cut(s) 366
Mph1103I ATGCAT 1 cut(s) 1099
MroXI GAANNNNTTC 1 cut(s) 135
MscI TGGCCA 1 cut(s) 366
MseI TTAA 7 cut(s) 149, 263, 347, 432, 534, 824, 839
MslI CAYNNNNRTG 2 cut(s) 1110, 1287
Msp20I TGGCCA 1 cut(s) 366
MspA1I CMGCKG 1 cut(s) 980
MspI CCGG 2 cut(s) 1169, 1191
MspR9I CCNGG 3 cut(s) 960, 1169, 1192
Mva1269I GAATGC 1 cut(s) 1432
MvaI CCWGG 1 cut(s) 960
MwoI GCNNNNNNNGC 3 cut(s) 395, 1015, 1399
NciI CCSGG 2 cut(s) 1169, 1192
NdeII GATC 3 cut(s) 628, 685, 917
NlaIII CATG 7 cut(s) 914, 924, 947, 1147, 1303, 1319, 1417
NmuCI GTSAC 3 cut(s) 3, 1103, 1416
NsiI ATGCAT 1 cut(s) 1099
PaeR7I CTCGAG 2 cut(s) 140, 1483
PctI GAATGC 1 cut(s) 1432
PdmI GAANNNNTTC 1 cut(s) 135
PfeI GAWTC 3 cut(s) 75, 245, 253
PfoI TCCNGGA 1 cut(s) 958
PkrI GCNGC 1 cut(s) 50
PleI GAGTC 4 cut(s) 226, 603, 676, 1110
PpsI GAGTC 4 cut(s) 226, 603, 676, 1110
PshBI ATTAAT 1 cut(s) 347
Psp1406I AACGTT 1 cut(s) 1155
Psp6I CCWGG 1 cut(s) 958
PspGI CCWGG 1 cut(s) 958
PspPI GGNCC 2 cut(s) 383, 907
PsrI GAACNNNNNNTAC 2 cut(s) 513, 545
PvuII CAGCTG 1 cut(s) 980
RsaI GTAC 4 cut(s) 618, 704, 871, 950
RsaNI GTAC 4 cut(s) 617, 703, 870, 949
RseI CAYNNNNRTG 2 cut(s) 1110, 1287
SaqAI TTAA 7 cut(s) 149, 263, 347, 432, 534, 824, 839
SatI GCNGC 1 cut(s) 49
Sau3AI GATC 3 cut(s) 628, 685, 917
Sau96I GGNCC 2 cut(s) 383, 907
SchI GAGTC 4 cut(s) 226, 603, 677, 1111
ScrFI CCNGG 3 cut(s) 960, 1169, 1192
SduI GDGCHC 1 cut(s) 147
SfaNI GCATC 5 cut(s) 205, 216, 398, 721, 1084
SfcI CTRYAG 1 cut(s) 257
Sfr274I CTCGAG 2 cut(s) 140, 1483
SinI GGWCC 2 cut(s) 383, 907
SlaI CTCGAG 2 cut(s) 140, 1483
SmiMI CAYNNNNRTG 2 cut(s) 1110, 1287
SmlI CTYRAG 5 cut(s) 140, 1002, 1071, 1176, 1483
SmoI CTYRAG 5 cut(s) 140, 1002, 1071, 1176, 1483
Sse9I AATT 8 cut(s) 39, 150, 179, 369, 648, 790, 897, 1089
SsiI CCGC 5 cut(s) 48, 396, 527, 1076, 1379
SspMI CTAG 5 cut(s) 200, 582, 591, 929, 1364
StyD4I CCNGG 3 cut(s) 958, 1167, 1190
TaaI ACNGT 6 cut(s) 115, 261, 437, 811, 1238, 1286
TaiI ACGT 2 cut(s) 996, 1158
TaqI TCGA 8 cut(s) 37, 129, 141, 405, 691, 732, 916, 1484
TaqII GACCGA 1 cut(s) 1423
TasI AATT 8 cut(s) 39, 150, 179, 369, 648, 790, 897, 1089
TatI WGTACW 1 cut(s) 702
TauI GCSGC 1 cut(s) 51
TfiI GAWTC 3 cut(s) 75, 245, 253
Tru1I TTAA 7 cut(s) 149, 263, 347, 432, 534, 824, 839
Tru9I TTAA 7 cut(s) 149, 263, 347, 432, 534, 824, 839
TscAI CASTG 5 cut(s) 442, 814, 1189, 1291, 1483
TseFI GTSAC 3 cut(s) 3, 1103, 1416
Tsp45I GTSAC 3 cut(s) 3, 1103, 1416
TspDTI ATGAA 6 cut(s) 336, 573, 750, 1128, 1341, 1402
TspGWI ACGGA 1 cut(s) 1245
TspRI CASTG 5 cut(s) 442, 814, 1189, 1291, 1483
VpaK11BI GGWCC 2 cut(s) 383, 907
VspI ATTAAT 1 cut(s) 347
XapI RAATTY 2 cut(s) 369, 790
XbaI TCTAGA 1 cut(s) 928
XhoI CTCGAG 2 cut(s) 140, 1483
XmnI GAANNNNTTC 1 cut(s) 135
XspI CTAG 5 cut(s) 200, 582, 591, 929, 1364
Zsp2I ATGCAT 1 cut(s) 1099
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.