Basic Information

Type: gene

Biological Identity

Species

rosa_laevigata

Chromosome

Chr1

Orthogroup ID

OG0004999

Physical Location & Seq

Genomic Coordinates Forward (+)

66872744 .. 66876279

Gene Structure 3536 bp

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UTR

Exon/CDS

Intron

Transcript / Protein ID

RLM00000005336

Sequence Viewer

CDS Protein Promoter (2k)

Length: 1710 bp

ATGTCGCAGAAGAGACACCAGGATGATGGCAAGGGGAGTTTTCGATCAGAAGGGACTGCTAGTCCGGGGGATAAGCGCCGGAGGACTACTTTTCACAATGTGGTTTCGGAAATAATGAAGTTGCACACTGTCCAGCATTTGCTGGAGCCAATTCTTGAGCCTTTGATTCGCAGAGTGGTCAGAGAGGAAGTAGAATTGGCCCTAAGGAAACATTTGAACAATATGAAACAGAATTGTGAGAAAGAGACTCAACCTTCTGAATCAAGAATCTTAAAGCTACAGTTCTTAAACAGTCTGTCTCTTCCGGTATTTACTGGAGCTCGGATTGAAGGAGAAGAGAGTTCCAGTGTCCAAGTAGCTTTGATTGATTGCTTTACTGGCCAAATAGTTAAATCTGGCCCTGAATCTGCAGCTAAAGTGGAAATTGTTGTCCTTGAGGGTGATTTTGATGGTGAGGAGGGTGACAATTGGACCCCTGAAGAGTTCAAGAATAACATTGTAAGAGAGAGGGAAGGCAAAAAACCTCTTCTAACAGGGGAAGCAGTCATCAATCTTAATGATGGAGTTGTTTCTGTGAGTGAGATTTCTTTTACAGATAATTCAAGCTGGACAAGGAGCCGTAGGTTCAGGTTAGGAGCAAGAGTTGTAGATAACTTTGATGGAACTAGAGTGAGAGAAGCAAAGACAGAATCCTTTATTGTCAGGGATCACCGTGGAGAATTGTACAAGAAGCATCACCCTCCATCTCTGCTTGATGAAGTATGGCGACTAGAAAAGATTGGAAAGGATGGAGCTTTCCACAAGCGTTTGACTCGGGAAAACATCCGCACCGTGAAGGATTTCCTCACCCTACTCTGCATAAACCCTCCAAGGCTCCGCCATATCCTTGGCACAGGTATGTCTACTAAGATGTGGGAAGTCACAGTGGAGCATGCTCAGACATGCATACTTGATAAGAGGATATACTTGTACTGCCCTCCCAGTTCACAACAGAGAACTGGTGTGGTCTTCAACGTTGTAGGACAAGTAACATACCTACTTTTGGAATGTGAATATATTCCTCTCAGTAAGCTTTCTGAAGCACAAAAGGTTGATGCCCAGAACTTGGTATTTTCTGCATTTGAACACTGGGGAGAAGTAGTCTCATTTGATGATGAGGCTTCTCTTTTGGGTGGGGGCTCATCTAATGTCGCCAATGTTCATCAGACATCAAGTTCACCAAGAGGAGAGGAGTTTACTGGAAGCAAGTTTATGACTACTCAAAAAATTGGCGGATTTGATTATACACAGGCATCCGCCTCTTCTCCTGATATCATTTCATCCATGTATTCTGTGGGGGGCACAAGTGGCTTGGATGATTATGCCTTGCACAACATGGATGGAATGAGTCTTAGATATGACCAGAGTTTAAGCTTCCCAGGTCAAGTTGGGAATTCCCTGATCTGTGACCCAGATTCAATAGCTGCATTTTGTGATGATGATCATCTACAGTTTTTTGATACTGATCTTCAGTCCCAGAGCATGCTTCCAGAGTCACCAGCAGATCTACAAAGTGCTGTAGATGGCTTCTTGTTGGCACAACGTTCTACTGCTGCTGTTGCCGCCATAGATAAGGCTCAGAGGAGATGGACAAAACTATTCAGTGTCCTCAAGTGGTTCTCAATTCAGAGGAGCGTGCGGAACCGGGTTCGAGACATTTCAAGATACTAG

Functional Annotation

Gene Ontology

Molecular Function
Biological Process
Cellular Component

GO:0002682 GO:0002683 GO:0002831 GO:0002832 GO:0003674 GO:0005488 GO:0005515 GO:0005516 GO:0008150 GO:0031347 GO:0031348 GO:0032101 GO:0032102 GO:0043900 GO:0043901 GO:0045088 GO:0045824 GO:0048519 GO:0048583 GO:0048585 GO:0050776 GO:0050777 GO:0050789 GO:0065007 GO:0080134 GO:1900424 GO:1900425 GO:1902477 GO:1902478

KEGG Pathways

Metabolic & Signaling

No pathways identified.

Pfam Domains

Protein Families

Calmodulin_bind

Protein Analysis

570

Amino Acids

63.92

Weight (kDa)

6.06

Isoelectric Point (pI)

52.18

Instability Index

Protein Domains (Pfam)

Domain Name	Pfam ID	Position	E-value	Description
Calmodulin_bind	PF07887	91 - 237	3.4e-61	Calmodulin binding protein-like N-terminal domain
Calmod_bind_M	PF20451	250 - 315	4.8e-32	Calmodulin binding protein central domain
Calmod_bind_C	PF20452	321 - 382	6.1e-16	Calmodulin binding protein C-terminal domain

Hydrophobicity Profile (Kyte-Doolittle)

AI Structure Prediction Report

Calculating structure properties...

Genomic Context

Gene Family Tree

Species Filter

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Align Labels (Cladogram)

Image

Tree File

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Publication-ready

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Orthologous Genes (Group: OG0004999)

Species	Orthologous Gene IDs
arabidopsis_thaliana	AT5G62570 AT5G62570 AT5G62570 AT5G62570
fragaria_vesca	FvH4_5g16470
malus_domestica	MD06G1180400.v1.1 MD14G1185500.v1.1
prunus_persica	Prupe.5G178300_v2.0.a1 Prupe.5G178300_v2.0.a1 Prupe.5G178300_v2.0.a1 Prupe.5G178300_v2.0.a1 Prupe.5G178300_v2.0.a1 Prupe.5G178300_v2.0.a1 Prupe.5G178300_v2.0.a1 Prupe.5G178300_v2.0.a1
pyrus_communis	pycom06g15980 pycom14g15440 pycom14g15900
rosa_chinensis	RchiOBHm_Chr7g0179621
rosa_laevigata	RLG00000005336
rosa_multiflora	Rmu_co8246235.1_g000001 Rmu_sc0005329.1_g000026
rosa_roxburghii	Rroxscaffold_155G00443860 Rroxscaffold_3G00273590
rosa_rugosa	Rorug06G0426700
rosa_samantha	Rh7AG026100 Rh7BG025300 Rh7CG027400 Rh7DG026900
rosa_wichuraiana	Rw7G002100

Restriction Enzyme Sites

1 / 10

Enzyme	Recognition Site	Cut Count	Positions (bp)
AccB7I	CCANNNNNTGG	1 cut(s)	1105
AccI	GTMKAC	1 cut(s)	902
AciI	CCGC	6 cut(s)	826, 877, 1272, 1296, 1602, 1678
AclI	AACGTT	2 cut(s)	1014, 1582
AclWI	GGATC	1 cut(s)	714
AcoI	YGGCCR	1 cut(s)	379
AcsI	RAATTY	1 cut(s)	1432
AcuI	CTGAAG	3 cut(s)	498, 1098, 1493
AdeI	CACNNNGTG	1 cut(s)	100
AfaI	GTAC	2 cut(s)	725, 971
AfiI	CCNNNNNNNGG	2 cut(s)	1042, 1105
AgsI	TTSAA	8 cut(s)	217, 329, 487, 603, 1012, 1124, 1458, 1701
AhdI	GACNNNNNGTC	1 cut(s)	60
AjnI	CCWGG	2 cut(s)	18, 1417
AluBI	AGCT	9 cut(s)	277, 320, 359, 413, 606, 794, 1072, 1413, 1463
AluI	AGCT	9 cut(s)	277, 320, 359, 413, 606, 794, 1072, 1413, 1463
Alw21I	GWGCWC	1 cut(s)	322
Alw26I	GTCTC	5 cut(s)	7, 239, 303, 1147, 1686
AlwI	GGATC	1 cut(s)	714
Ama87I	CYCGRG	1 cut(s)	813
AoxI	GGCC	3 cut(s)	198, 379, 397
ApeKI	GCWGC	3 cut(s)	410, 1463, 1592
ApoI	RAATTY	1 cut(s)	1432
Asp700I	GAANNNNTTC	2 cut(s)	839, 1056
AspLEI	GCGC	1 cut(s)	78
AspS9I	GGNCC	3 cut(s)	199, 398, 471
AsuC2I	CCSGG	2 cut(s)	66, 1685
AsuHPI	GGTGA	8 cut(s)	452, 464, 473, 701, 728, 838, 1209, 1527
AvaI	CYCGRG	1 cut(s)	813
AvaII	GGWCC	1 cut(s)	471
AxyI	CCTNAGG	1 cut(s)	203
BaeGI	GKGCMC	1 cut(s)	1343
BalI	TGGCCA	1 cut(s)	381
BanII	GRGCYC	2 cut(s)	322, 1181
BbsI	GAAGAC	1 cut(s)	1000
Bbv12I	GWGCWC	1 cut(s)	322
BbvI	GCAGC	3 cut(s)	422, 1450, 1579
BccI	CCATC	9 cut(s)	20, 443, 554, 653, 751, 782, 1373, 1556, 1620
BceAI	ACGGC	1 cut(s)	603
BciT130I	CCWGG	2 cut(s)	20, 1419
BclI	TGATCA	1 cut(s)	1480
BcnI	CCSGG	2 cut(s)	66, 1685
BcoDI	GTCTC	5 cut(s)	7, 239, 303, 1147, 1686
BfaI	CTAG	4 cut(s)	60, 666, 770, 1708
BfmI	CTRYAG	4 cut(s)	278, 408, 1487, 1557
BfoI	RGCGCY	1 cut(s)	79
BglII	AGATCT	1 cut(s)	1543
BisI	GCNGC	4 cut(s)	411, 1464, 1593, 1602
BlsI	GCNGC	4 cut(s)	412, 1465, 1594, 1603
Bme1390I	CCNGG	4 cut(s)	20, 66, 1419, 1685
Bme18I	GGWCC	1 cut(s)	471
BmeRI	GACNNNNNGTC	1 cut(s)	60
BmeT110I	CYCGRG	1 cut(s)	813
BmgT120I	GGNCC	3 cut(s)	199, 398, 471
BmiI	GGNNCC	5 cut(s)	147, 473, 617, 875, 1682
BmrFI	CCNGG	4 cut(s)	20, 66, 1419, 1685
BmrI	ACTGGG	2 cut(s)	975, 1138
BmsI	GCATC	3 cut(s)	742, 1084, 1301
BmuI	ACTGGG	2 cut(s)	975, 1138
BpiI	GAAGAC	1 cut(s)	1000
BpmI	CTGGAG	2 cut(s)	164, 336
BpuEI	CTTGAG	3 cut(s)	176, 455, 1634
BpuMI	CCSGG	2 cut(s)	66, 1685
BsaBI	GATNNNNATC	3 cut(s)	1479, 1482, 1503
BsaJI	CCNNGG	5 cut(s)	65, 712, 869, 886, 1417
BsaWI	WCCGGW	1 cut(s)	304
BsaXI	ACNNNNNCTCC	4 cut(s)	627, 657, 1125, 1155
Bsc4I	CCNNNNNNNGG	2 cut(s)	1042, 1105
Bse1I	ACTGG	7 cut(s)	319, 345, 382, 981, 1003, 1133, 1243
Bse21I	CCTNAGG	1 cut(s)	203
Bse8I	GATNNNNATC	3 cut(s)	1479, 1482, 1503
BseBI	CCWGG	2 cut(s)	20, 1419
BseDI	CCNNGG	5 cut(s)	65, 712, 869, 886, 1417
BseGI	GGATG	7 cut(s)	28, 793, 822, 1292, 1319, 1360, 1384
BseJI	GATNNNNATC	3 cut(s)	1479, 1482, 1503
BseLI	CCNNNNNNNGG	2 cut(s)	1042, 1105
BseMII	CTCAG	3 cut(s)	950, 1078, 1631
BseNI	ACTGG	7 cut(s)	319, 345, 382, 981, 1003, 1133, 1243
BseRI	GAGGAG	5 cut(s)	470, 1239, 1244, 1636, 1684
BseSI	GKGCMC	1 cut(s)	1343
BseXI	GCAGC	3 cut(s)	422, 1450, 1579
BshFI	GGCC	3 cut(s)	200, 381, 399
BsiHKAI	GWGCWC	1 cut(s)	322
BsiHKCI	CYCGRG	1 cut(s)	813
BsiSI	CCGG	4 cut(s)	65, 79, 305, 1684
BslFI	GGGAC	2 cut(s)	67, 1498
BslI	CCNNNNNNNGG	2 cut(s)	1042, 1105
BsmAI	GTCTC	5 cut(s)	7, 239, 303, 1147, 1686
BsmFI	GGGAC	2 cut(s)	67, 1498
BsnI	GGCC	3 cut(s)	200, 381, 399
BsoBI	CYCGRG	1 cut(s)	813
Bsp1286I	GDGCHC	3 cut(s)	322, 1181, 1343
Bsp1407I	TGTACA	1 cut(s)	723
Bsp143I	GATC	6 cut(s)	44, 706, 1440, 1480, 1504, 1543
BspACI	CCGC	6 cut(s)	826, 877, 1272, 1296, 1602, 1678
BspANI	GGCC	3 cut(s)	200, 381, 399
BspCNI	CTCAG	3 cut(s)	949, 1077, 1630
BspLI	GGNNCC	5 cut(s)	147, 473, 617, 875, 1682
BspMAI	CTGCAG	1 cut(s)	412
BspPI	GGATC	1 cut(s)	714
BsrGI	TGTACA	1 cut(s)	723
BsrI	ACTGG	7 cut(s)	319, 345, 382, 981, 1003, 1133, 1243
BssECI	CCNNGG	5 cut(s)	65, 712, 869, 886, 1417
BssMI	GATC	6 cut(s)	44, 706, 1440, 1480, 1504, 1543
BssT1I	CCWWGG	2 cut(s)	869, 886
Bst2UI	CCWGG	2 cut(s)	20, 1419
Bst4CI	ACNGT	7 cut(s)	130, 282, 293, 713, 832, 925, 1491
Bst6I	CTCTTC	6 cut(s)	5, 306, 330, 474, 531, 1306
BstAUI	TGTACA	1 cut(s)	723
BstC8I	GCNNGC	3 cut(s)	933, 1523, 1676
BstDEI	CTNAG	6 cut(s)	203, 906, 936, 1064, 1391, 1617
BstDSI	CCRYGG	1 cut(s)	712
BstF5I	GGATG	7 cut(s)	28, 793, 822, 1292, 1319, 1360, 1384
BstH2I	RGCGCY	1 cut(s)	79
BstHHI	GCGC	1 cut(s)	78
BstKTI	GATC	6 cut(s)	47, 709, 1443, 1483, 1507, 1546
BstMAI	GTCTC	5 cut(s)	7, 239, 303, 1147, 1686
BstMBI	GATC	6 cut(s)	44, 706, 1440, 1480, 1504, 1543
BstMWI	GCNNNNNNNGC	4 cut(s)	378, 1347, 1598, 1601
BstNI	CCWGG	2 cut(s)	20, 1419
BstNSI	RCATGY	3 cut(s)	935, 945, 1525
BstSCI	CCNGG	4 cut(s)	18, 64, 1417, 1683
BstSFI	CTRYAG	4 cut(s)	278, 408, 1487, 1557
BstSLI	GKGCMC	1 cut(s)	1343
BstV1I	GCAGC	3 cut(s)	422, 1450, 1579
BstV2I	GAAGAC	1 cut(s)	1000
BstX2I	RGATCY	1 cut(s)	1543
BstXI	CCANNNNNNTGG	2 cut(s)	26, 887
BstYI	RGATCY	1 cut(s)	1543
Bsu36I	CCTNAGG	1 cut(s)	203
BsuRI	GGCC	3 cut(s)	200, 381, 399
BtgI	CCRYGG	1 cut(s)	712
BtsCI	GGATG	7 cut(s)	28, 793, 822, 1292, 1319, 1360, 1384
BtsIMutI	CAGTG	5 cut(s)	126, 352, 930, 1126, 1648
Cac8I	GCNNGC	3 cut(s)	933, 1523, 1676
CfoI	GCGC	1 cut(s)	78
Cfr13I	GGNCC	3 cut(s)	199, 398, 471
Csp6I	GTAC	2 cut(s)	724, 970
CviAII	CATG	5 cut(s)	932, 942, 1324, 1375, 1522
CviQI	GTAC	2 cut(s)	724, 970
DdeI	CTNAG	6 cut(s)	203, 906, 936, 1064, 1391, 1617
DpnI	GATC	6 cut(s)	46, 708, 1442, 1482, 1506, 1545
DpnII	GATC	6 cut(s)	44, 706, 1440, 1480, 1504, 1543
DraIII	CACNNNGTG	1 cut(s)	100
DriI	GACNNNNNGTC	1 cut(s)	60
EaeI	YGGCCR	1 cut(s)	379
Eam1104I	CTCTTC	6 cut(s)	5, 306, 330, 474, 531, 1306
Eam1105I	GACNNNNNGTC	1 cut(s)	60
EarI	CTCTTC	6 cut(s)	5, 306, 330, 474, 531, 1306
EciI	GGCGGA	3 cut(s)	866, 1285, 1287
Ecl136II	GAGCTC	1 cut(s)	320
Eco130I	CCWWGG	2 cut(s)	869, 886
Eco24I	GRGCYC	2 cut(s)	322, 1181
Eco32I	GATATC	1 cut(s)	1312
Eco47I	GGWCC	1 cut(s)	471
Eco53kI	GAGCTC	1 cut(s)	320
Eco57I	CTGAAG	3 cut(s)	498, 1098, 1493
Eco81I	CCTNAGG	1 cut(s)	203
Eco88I	CYCGRG	1 cut(s)	813
EcoICRI	GAGCTC	1 cut(s)	320
EcoRI	GAATTC	1 cut(s)	1432
EcoRII	CCWGG	2 cut(s)	18, 1417
EcoRV	GATATC	1 cut(s)	1312
EcoT14I	CCWWGG	2 cut(s)	869, 886
EcoT22I	ATGCAT	1 cut(s)	947
EcoT38I	GRGCYC	2 cut(s)	322, 1181
ErhI	CCWWGG	2 cut(s)	869, 886
FaeI	CATG	5 cut(s)	935, 945, 1327, 1378, 1525
FaqI	GGGAC	2 cut(s)	67, 1498
FatI	CATG	5 cut(s)	931, 941, 1323, 1374, 1521
FbaI	TGATCA	1 cut(s)	1480
FblI	GTMKAC	1 cut(s)	902
Fnu4HI	GCNGC	4 cut(s)	411, 1464, 1593, 1602
FokI	GGATG	7 cut(s)	35, 800, 809, 1279, 1306, 1367, 1391
FriOI	GRGCYC	2 cut(s)	322, 1181
Fsp4HI	GCNGC	4 cut(s)	411, 1464, 1593, 1602
FspBI	CTAG	4 cut(s)	60, 666, 770, 1708
GlaI	GCGC	1 cut(s)	77
GluI	GCNGC	4 cut(s)	411, 1464, 1593, 1602
GsuI	CTGGAG	2 cut(s)	164, 336
HaeII	RGCGCY	1 cut(s)	79
HaeIII	GGCC	3 cut(s)	200, 381, 399
HapII	CCGG	4 cut(s)	65, 79, 305, 1684
HhaI	GCGC	1 cut(s)	78
Hin1II	CATG	5 cut(s)	935, 945, 1327, 1378, 1525
Hin6I	GCGC	1 cut(s)	76
HinP1I	GCGC	1 cut(s)	76
HindIII	AAGCTT	2 cut(s)	1070, 1411
HpaII	CCGG	4 cut(s)	65, 79, 305, 1684
HphI	GGTGA	8 cut(s)	452, 464, 473, 701, 728, 838, 1209, 1527
Hpy166II	GTNNAC	4 cut(s)	903, 986, 1217, 1236
Hpy188III	TCNNGA	8 cut(s)	155, 264, 487, 815, 1307, 1529, 1691, 1701
Hpy8I	GTNNAC	4 cut(s)	903, 986, 1217, 1236
HpyAV	CCTTC	5 cut(s)	44, 264, 323, 506, 829
HpyCH4III	ACNGT	7 cut(s)	130, 282, 293, 713, 832, 925, 1491
HpyCH4IV	ACGT	2 cut(s)	1014, 1582
HpyCH4V	TGCA	7 cut(s)	124, 410, 858, 945, 1118, 1369, 1466
HpyF10VI	GCNNNNNNNGC	4 cut(s)	378, 1347, 1598, 1601
HpyF3I	CTNAG	6 cut(s)	203, 906, 936, 1064, 1391, 1617
HpySE526I	ACGT	2 cut(s)	1014, 1582
Hsp92II	CATG	5 cut(s)	935, 945, 1327, 1378, 1525
HspAI	GCGC	1 cut(s)	76
Ksp22I	TGATCA	1 cut(s)	1480
Kzo9I	GATC	6 cut(s)	44, 706, 1440, 1480, 1504, 1543
LmnI	GCTCC	8 cut(s)	145, 317, 615, 635, 791, 879, 928, 1671
Lsp1109I	GCAGC	3 cut(s)	422, 1450, 1579
LweI	GCATC	3 cut(s)	742, 1084, 1301
MaeI	CTAG	4 cut(s)	60, 666, 770, 1708
MaeII	ACGT	2 cut(s)	1014, 1582
MaeIII	GTNAC	5 cut(s)	461, 919, 1027, 1445, 1533
MalI	GATC	6 cut(s)	46, 708, 1442, 1482, 1506, 1545
MboI	GATC	6 cut(s)	44, 706, 1440, 1480, 1504, 1543
MboII	GAAGA	8 cut(s)	22, 293, 347, 491, 518, 1000, 1293, 1499
MfeI	CAATTG	1 cut(s)	466
MflI	RGATCY	1 cut(s)	1543
MhlI	GDGCHC	3 cut(s)	322, 1181, 1343
MlsI	TGGCCA	1 cut(s)	381
MluNI	TGGCCA	1 cut(s)	381
MlyI	GAGTC	4 cut(s)	241, 805, 1396, 1541
Mox20I	TGGCCA	1 cut(s)	381
Mph1103I	ATGCAT	1 cut(s)	947
MroXI	GAANNNNTTC	2 cut(s)	839, 1056
MscI	TGGCCA	1 cut(s)	381
MseI	TTAA	5 cut(s)	272, 287, 390, 555, 1409
MslI	CAYNNNNRTG	2 cut(s)	21, 896
Msp20I	TGGCCA	1 cut(s)	381
MspI	CCGG	4 cut(s)	65, 79, 305, 1684
MspR9I	CCNGG	4 cut(s)	20, 66, 1419, 1685
MunI	CAATTG	1 cut(s)	466
MvaI	CCWGG	2 cut(s)	20, 1419
MwoI	GCNNNNNNNGC	4 cut(s)	378, 1347, 1598, 1601
NciI	CCSGG	2 cut(s)	66, 1685
NdeII	GATC	6 cut(s)	44, 706, 1440, 1480, 1504, 1543
NlaIII	CATG	5 cut(s)	935, 945, 1327, 1378, 1525
NlaIV	GGNNCC	5 cut(s)	147, 473, 617, 875, 1682
NmuCI	GTSAC	4 cut(s)	461, 919, 1445, 1533
NsiI	ATGCAT	1 cut(s)	947
NspI	RCATGY	3 cut(s)	935, 945, 1525
PaeI	GCATGC	2 cut(s)	935, 1525
PdmI	GAANNNNTTC	2 cut(s)	839, 1056
PfeI	GAWTC	6 cut(s)	166, 260, 267, 404, 689, 1454
PflMI	CCANNNNNTGG	1 cut(s)	1105
PkrI	GCNGC	4 cut(s)	412, 1465, 1594, 1603
PleI	GAGTC	4 cut(s)	241, 805, 1395, 1540
PpsI	GAGTC	4 cut(s)	241, 805, 1395, 1540
Psp124BI	GAGCTC	1 cut(s)	322
Psp1406I	AACGTT	2 cut(s)	1014, 1582
Psp6I	CCWGG	2 cut(s)	18, 1417
PspGI	CCWGG	2 cut(s)	18, 1417
PspN4I	GGNNCC	5 cut(s)	147, 473, 617, 875, 1682
PspPI	GGNCC	3 cut(s)	199, 398, 471
PstI	CTGCAG	1 cut(s)	412
PsuI	RGATCY	1 cut(s)	1543
RsaI	GTAC	2 cut(s)	725, 971
RsaNI	GTAC	2 cut(s)	724, 970
RseI	CAYNNNNRTG	2 cut(s)	21, 896
SacI	GAGCTC	1 cut(s)	322
SaqAI	TTAA	5 cut(s)	272, 287, 390, 555, 1409
SatI	GCNGC	4 cut(s)	411, 1464, 1593, 1602
Sau3AI	GATC	6 cut(s)	44, 706, 1440, 1480, 1504, 1543
Sau96I	GGNCC	3 cut(s)	199, 398, 471
SchI	GAGTC	4 cut(s)	241, 805, 1396, 1541
ScrFI	CCNGG	4 cut(s)	20, 66, 1419, 1685
SduI	GDGCHC	3 cut(s)	322, 1181, 1343
SfaNI	GCATC	3 cut(s)	742, 1084, 1301
SfcI	CTRYAG	4 cut(s)	278, 408, 1487, 1557
SinI	GGWCC	1 cut(s)	471
SmiMI	CAYNNNNRTG	2 cut(s)	21, 896
SmlI	CTYRAG	3 cut(s)	155, 434, 1649
SmoI	CTYRAG	3 cut(s)	155, 434, 1649
SphI	GCATGC	2 cut(s)	935, 1525
SsiI	CCGC	6 cut(s)	826, 877, 1272, 1296, 1602, 1678
SspMI	CTAG	4 cut(s)	60, 666, 770, 1708
SstI	GAGCTC	1 cut(s)	322
StyD4I	CCNGG	4 cut(s)	18, 64, 1417, 1683
StyI	CCWWGG	2 cut(s)	869, 886
TaaI	ACNGT	7 cut(s)	130, 282, 293, 713, 832, 925, 1491
TaiI	ACGT	2 cut(s)	1017, 1585
TaqI	TCGA	2 cut(s)	43, 1690
TatI	WGTACW	2 cut(s)	723, 969
TauI	GCSGC	1 cut(s)	1604
TfiI	GAWTC	6 cut(s)	166, 260, 267, 404, 689, 1454
Tru1I	TTAA	5 cut(s)	272, 287, 390, 555, 1409
Tru9I	TTAA	5 cut(s)	272, 287, 390, 555, 1409
TscAI	CASTG	5 cut(s)	133, 352, 930, 1133, 1648
TseFI	GTSAC	4 cut(s)	461, 919, 1445, 1533
TseI	GCWGC	3 cut(s)	410, 1463, 1592
Tsp45I	GTSAC	4 cut(s)	461, 919, 1445, 1533
TspDTI	ATGAA	5 cut(s)	131, 239, 771, 1190, 1308
TspRI	CASTG	5 cut(s)	133, 352, 930, 1133, 1648
Van91I	CCANNNNNTGG	1 cut(s)	1105
VpaK11BI	GGWCC	1 cut(s)	471
XapI	RAATTY	1 cut(s)	1432
XceI	RCATGY	3 cut(s)	935, 945, 1525
XcmI	CCANNNNNNNNNTGG	1 cut(s)	1330
XmiI	GTMKAC	1 cut(s)	902
XmnI	GAANNNNTTC	2 cut(s)	839, 1056
XspI	CTAG	4 cut(s)	60, 666, 770, 1708
Zsp2I	ATGCAT	1 cut(s)	947

Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.