FvH4_5g16470

Calmodulin binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
fragaria_vesca
Fvb5
OG0004999
Physical Location & Seq
Forward (+)
9369736 .. 9373934
4199 bp
Loading structure...
UTR
Exon/CDS
Intron
FvH4_5g16470.t1

Sequence Viewer

Length: 1698 bp
ATGTCGCAGAAGAGACACCAAGATGATGGCAAGGCTCACCGATCAGAAGCCGAGGATAAGCGCCGGAGGACTACTTTTCAAAATGTGGTTTCGGAAATAATGAAGTTGCACACTGTTCAGCATTTGCTGGAGCCAATTCTTGAGCCATTGATTCGCAGAGTGGTCAGAGAGGAAGTAGAATCGGCCTTAAGGAAACATTTGAACAATATGAAACAGAATTGTGAGATAGAGACTCAACCTTCTGAATCAAGAATCTTAAAGCTCCAGTTCTTGAATAGTCTGTCTCTTCCGGTATTTACTGGAGCTCGGATTGAAGGAGAAGAGAGTTCCAGTATCCAAATAGCTCTGGTTGATTACTTTACTGGCCAAGTAGTTGAATCTGGCCCAGAATCCTCAGCTAAGGTGGAAATTGTTGTTCTTGAGGGTGATTTTGATGGTGAGGAGGGTGATAATTGGACCCCTGAAGAGTTCAAGAATAACATTGTAAGAGAGAGGGAAGGCAAAAAACCTCTTCTAACAGGGGAACCAGCCATCAATCTTAATGATGGAGTTGGTTCTGTGAGTGAGATTTCTTTTACAGATAATTCAAGCTGGACAAGGAGCCGTAGGTTCAGGTTAGGAGCAAGAGTTGTAGATAATTTTGATGGAACTAGAGTAAGAGAAGCAAAGACAGAATCCTTTATTGTTAGGGATCACCGTGGAGAATTGTACAAGAAGCACCACCCTCCATCCCTGCTTGATGAAGTATGGCGACTAGAAAAGATTGGAAAGGACGGTGCTTTCCATAAGCGTTTGACTCGGGAAAACATCCGCACCGTGAAGGATTTCCTCATCCTACTCTTCATAAACCCTCCAAGACTCCGCCATATCCTTGGCACTGGTATGTCTACTAAGATGTGGGAAGTCACAGTGGAGCATGCTCAGACATGTATACTGGATAAGAGGATATACTTGTACTGCCCTCCTGGTTCACAACAGAGAACTGGTGTGGTCTTCAACGTCGTTGGACAAGTAATGTACCTACTTTTGGAATGTGAATATATTCCTGTGAGTAAGCTTTCTGAAACACAAAAGGCTGATGCCCAGAACTTGGTACTTTCTGCATTTGAACACTGGGGAGAAGTTGTCTCATTTGATGATGAAGCTTCTCTTTTGGGAGGGGGCTCGTCCAATGTTGCCAATGTTCTTCAGACATCAAGCTCACCAAGAGGAGAGGATTTTAATGGAAGCAAGTTTTTGGCTACGCAAAAAATTGGTGGATTTGATTATACACAGCCACCCGCCTCTTCTCCTGATATCATTTCATCCATATATTCTGTTGGGGGCACAAGTGGCTTGGATGATTATGCCTTGCACAACATTGATGGAATGAGTCTTAGATATGACCAGGCTTTAACGTTCCCAGGTCAAGTTGGGAATTCCTTGATCTGCGATCCAGATTCAATAGCTCATGCATTTTGTGATGACGATCATCTACAGTTTTTCGATACTGATCTTCAGTCCCAGAACATGCTTCCAGAGTCACCAGCAGATCTACAAAGTGCTGTAGATGGCTTCCTGTTGGCACAACGTTCTACTGCTGCTGTTTCCGCCATAGATAAGGCTCAGAGGAGATGGACAAAACTATTCAGTGTCCTCAAGTGGTTCTCAATTCGGAGGAGCGTACGGAACCGGGTTCGAGACATTCCAAGATACTAG

Protein Analysis

566

Amino Acids

63.7

Weight (kDa)

5.83

Isoelectric Point (pI)

55.61

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 86 - 232 1.3e-62 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 245 - 310 9.9e-32 Calmodulin binding protein central domain
Calmod_bind_C PF20452 316 - 377 7.2e-18 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1090
AccI GTMKAC 2 cut(s) 887, 931
AciI CCGC 4 cut(s) 811, 862, 1281, 1590
AclI AACGTT 2 cut(s) 1397, 1570
AclWI GGATC 2 cut(s) 699, 1427
AcoI YGGCCR 1 cut(s) 364
AcsI RAATTY 1 cut(s) 1417
AcuI CTGAAG 3 cut(s) 483, 1172, 1481
AfaI GTAC 5 cut(s) 710, 956, 1019, 1095, 1665
AfiI CCNNNNNNNGG 2 cut(s) 1027, 1090
AflII CTTAAG 1 cut(s) 187
AflIII ACRYGT 1 cut(s) 926
AjnI CCWGG 3 cut(s) 964, 1386, 1402
AluBI AGCT 9 cut(s) 262, 305, 344, 398, 591, 1057, 1145, 1200, 1448
AluI AGCT 9 cut(s) 262, 305, 344, 398, 591, 1057, 1145, 1200, 1448
Alw21I GWGCWC 1 cut(s) 307
Alw26I GTCTC 5 cut(s) 7, 224, 288, 1132, 1674
AlwI GGATC 2 cut(s) 699, 1427
Ama87I CYCGRG 1 cut(s) 798
AoxI GGCC 3 cut(s) 183, 364, 382
ApeKI GCWGC 1 cut(s) 1580
ApoI RAATTY 1 cut(s) 1417
Asp700I GAANNNNTTC 2 cut(s) 824, 1041
AspLEI GCGC 1 cut(s) 63
AspS9I GGNCC 2 cut(s) 383, 456
AsuC2I CCSGG 1 cut(s) 1673
AsuHPI GGTGA 7 cut(s) 29, 437, 449, 458, 686, 1194, 1515
AvaI CYCGRG 1 cut(s) 798
AvaII GGWCC 1 cut(s) 456
BaeGI GKGCMC 1 cut(s) 1328
BalI TGGCCA 1 cut(s) 366
BanII GRGCYC 2 cut(s) 307, 1166
BbsI GAAGAC 1 cut(s) 985
Bbv12I GWGCWC 1 cut(s) 307
BbvCI CCTCAGC 1 cut(s) 394
BbvI GCAGC 1 cut(s) 1567
BccI CCATC 9 cut(s) 20, 428, 539, 539, 638, 736, 1358, 1544, 1608
BceAI ACGGC 1 cut(s) 588
BciT130I CCWGG 3 cut(s) 966, 1388, 1404
BciVI GTATCC 1 cut(s) 344
BcnI CCSGG 1 cut(s) 1673
BcoDI GTCTC 5 cut(s) 7, 224, 288, 1132, 1674
BfaI CTAG 3 cut(s) 651, 755, 1696
BfmI CTRYAG 2 cut(s) 1475, 1545
BfoI RGCGCY 1 cut(s) 64
BfrI CTTAAG 1 cut(s) 187
BfuI GTATCC 1 cut(s) 344
BglII AGATCT 1 cut(s) 1531
BisI GCNGC 1 cut(s) 1581
BlsI GCNGC 1 cut(s) 1582
Bme1390I CCNGG 4 cut(s) 966, 1388, 1404, 1673
Bme18I GGWCC 1 cut(s) 456
BmeT110I CYCGRG 1 cut(s) 798
BmgT120I GGNCC 2 cut(s) 383, 456
BmiI GGNNCC 5 cut(s) 132, 458, 525, 602, 1670
BmrFI CCNGG 4 cut(s) 966, 1388, 1404, 1673
BmrI ACTGGG 1 cut(s) 1123
BmsI GCATC 1 cut(s) 1069
BmuI ACTGGG 1 cut(s) 1123
BpiI GAAGAC 1 cut(s) 985
BpmI CTGGAG 3 cut(s) 149, 248, 321
Bpu10I CCTNAGC 2 cut(s) 394, 399
BpuEI CTTGAG 3 cut(s) 161, 440, 1622
BpuMI CCSGG 1 cut(s) 1673
BsaBI GATNNNNATC 2 cut(s) 1467, 1491
BsaJI CCNNGG 4 cut(s) 51, 697, 871, 1402
BsaWI WCCGGW 1 cut(s) 289
BsaXI ACNNNNNCTCC 4 cut(s) 612, 642, 1110, 1140
Bsc4I CCNNNNNNNGG 2 cut(s) 1027, 1090
Bse1I ACTGG 8 cut(s) 265, 304, 330, 367, 883, 939, 988, 1118
Bse8I GATNNNNATC 2 cut(s) 1467, 1491
BseBI CCWGG 3 cut(s) 966, 1388, 1404
BseDI CCNNGG 4 cut(s) 51, 697, 871, 1402
BseGI GGATG 5 cut(s) 728, 807, 831, 1304, 1345
BseJI GATNNNNATC 2 cut(s) 1467, 1491
BseLI CCNNNNNNNGG 2 cut(s) 1027, 1090
BseMII CTCAG 3 cut(s) 408, 935, 1619
BseNI ACTGG 8 cut(s) 265, 304, 330, 367, 883, 939, 988, 1118
BseRI GAGGAG 4 cut(s) 455, 1224, 1624, 1672
BseSI GKGCMC 1 cut(s) 1328
BseXI GCAGC 1 cut(s) 1567
BshFI GGCC 3 cut(s) 185, 366, 384
BsiHKAI GWGCWC 1 cut(s) 307
BsiHKCI CYCGRG 1 cut(s) 798
BsiSI CCGG 3 cut(s) 64, 290, 1672
BsiWI CGTACG 1 cut(s) 1663
BslFI GGGAC 1 cut(s) 1486
BslI CCNNNNNNNGG 2 cut(s) 1027, 1090
BsmAI GTCTC 5 cut(s) 7, 224, 288, 1132, 1674
BsmFI GGGAC 1 cut(s) 1486
BsnI GGCC 3 cut(s) 185, 366, 384
BsoBI CYCGRG 1 cut(s) 798
Bsp1286I GDGCHC 3 cut(s) 307, 1166, 1328
Bsp1407I TGTACA 1 cut(s) 708
Bsp143I GATC 7 cut(s) 41, 691, 1425, 1432, 1468, 1492, 1531
BspACI CCGC 4 cut(s) 811, 862, 1281, 1590
BspANI GGCC 3 cut(s) 185, 366, 384
BspCNI CTCAG 3 cut(s) 407, 934, 1618
BspLI GGNNCC 5 cut(s) 132, 458, 525, 602, 1670
BspPI GGATC 2 cut(s) 699, 1427
BspTI CTTAAG 1 cut(s) 187
BsrGI TGTACA 1 cut(s) 708
BsrI ACTGG 8 cut(s) 265, 304, 330, 367, 883, 939, 988, 1118
BssECI CCNNGG 4 cut(s) 51, 697, 871, 1402
BssMI GATC 7 cut(s) 41, 691, 1425, 1432, 1468, 1492, 1531
BssNAI GTATAC 1 cut(s) 932
BssT1I CCWWGG 1 cut(s) 871
Bst1107I GTATAC 1 cut(s) 932
Bst2UI CCWGG 3 cut(s) 966, 1388, 1404
Bst4CI ACNGT 6 cut(s) 115, 698, 776, 817, 910, 1479
Bst6I CTCTTC 7 cut(s) 5, 291, 315, 459, 516, 845, 1291
BstAFI CTTAAG 1 cut(s) 187
BstAUI TGTACA 1 cut(s) 708
BstC8I GCNNGC 1 cut(s) 918
BstDEI CTNAG 6 cut(s) 394, 399, 891, 921, 1376, 1605
BstDSI CCRYGG 1 cut(s) 697
BstF5I GGATG 5 cut(s) 728, 807, 831, 1304, 1345
BstH2I RGCGCY 1 cut(s) 64
BstHHI GCGC 1 cut(s) 63
BstKTI GATC 7 cut(s) 44, 694, 1428, 1435, 1471, 1495, 1534
BstMAI GTCTC 5 cut(s) 7, 224, 288, 1132, 1674
BstMBI GATC 7 cut(s) 41, 691, 1425, 1432, 1468, 1492, 1531
BstMWI GCNNNNNNNGC 2 cut(s) 1332, 1589
BstNI CCWGG 3 cut(s) 966, 1388, 1404
BstNSI RCATGY 3 cut(s) 920, 930, 1513
BstSCI CCNGG 4 cut(s) 964, 1386, 1402, 1671
BstSFI CTRYAG 2 cut(s) 1475, 1545
BstSLI GKGCMC 1 cut(s) 1328
BstV1I GCAGC 1 cut(s) 1567
BstV2I GAAGAC 1 cut(s) 985
BstX2I RGATCY 1 cut(s) 1531
BstXI CCANNNNNNTGG 2 cut(s) 26, 872
BstYI RGATCY 1 cut(s) 1531
BstZ17I GTATAC 1 cut(s) 932
BsuI GTATCC 1 cut(s) 344
BsuRI GGCC 3 cut(s) 185, 366, 384
BtgI CCRYGG 1 cut(s) 697
BtsCI GGATG 5 cut(s) 728, 807, 831, 1304, 1345
BtsIMutI CAGTG 5 cut(s) 111, 876, 915, 1111, 1636
Cac8I GCNNGC 1 cut(s) 918
CfoI GCGC 1 cut(s) 63
Cfr13I GGNCC 2 cut(s) 383, 456
Csp6I GTAC 5 cut(s) 709, 955, 1018, 1094, 1664
CviAII CATG 4 cut(s) 917, 927, 1451, 1510
CviQI GTAC 5 cut(s) 709, 955, 1018, 1094, 1664
DdeI CTNAG 6 cut(s) 394, 399, 891, 921, 1376, 1605
DpnI GATC 7 cut(s) 43, 693, 1427, 1434, 1470, 1494, 1533
DpnII GATC 7 cut(s) 41, 691, 1425, 1432, 1468, 1492, 1531
EaeI YGGCCR 1 cut(s) 364
Eam1104I CTCTTC 7 cut(s) 5, 291, 315, 459, 516, 845, 1291
EarI CTCTTC 7 cut(s) 5, 291, 315, 459, 516, 845, 1291
EciI GGCGGA 2 cut(s) 851, 1579
Ecl136II GAGCTC 1 cut(s) 305
Eco130I CCWWGG 1 cut(s) 871
Eco24I GRGCYC 2 cut(s) 307, 1166
Eco32I GATATC 1 cut(s) 1297
Eco47I GGWCC 1 cut(s) 456
Eco53kI GAGCTC 1 cut(s) 305
Eco57I CTGAAG 3 cut(s) 483, 1172, 1481
Eco88I CYCGRG 1 cut(s) 798
EcoICRI GAGCTC 1 cut(s) 305
EcoRI GAATTC 1 cut(s) 1417
EcoRII CCWGG 3 cut(s) 964, 1386, 1402
EcoRV GATATC 1 cut(s) 1297
EcoT14I CCWWGG 1 cut(s) 871
EcoT22I ATGCAT 1 cut(s) 1456
EcoT38I GRGCYC 2 cut(s) 307, 1166
ErhI CCWWGG 1 cut(s) 871
FaeI CATG 4 cut(s) 920, 930, 1454, 1513
FalI AAGNNNNNCTT 2 cut(s) 1134, 1166
FaqI GGGAC 1 cut(s) 1486
FatI CATG 4 cut(s) 916, 926, 1450, 1509
FauI CCCGC 1 cut(s) 1288
FblI GTMKAC 2 cut(s) 887, 931
Fnu4HI GCNGC 1 cut(s) 1581
FokI GGATG 5 cut(s) 715, 794, 818, 1291, 1352
FriOI GRGCYC 2 cut(s) 307, 1166
Fsp4HI GCNGC 1 cut(s) 1581
FspBI CTAG 3 cut(s) 651, 755, 1696
GlaI GCGC 1 cut(s) 62
GluI GCNGC 1 cut(s) 1581
GsuI CTGGAG 3 cut(s) 149, 248, 321
HaeII RGCGCY 1 cut(s) 64
HaeIII GGCC 3 cut(s) 185, 366, 384
HapII CCGG 3 cut(s) 64, 290, 1672
HhaI GCGC 1 cut(s) 63
Hin1II CATG 4 cut(s) 920, 930, 1454, 1513
Hin6I GCGC 1 cut(s) 61
HinP1I GCGC 1 cut(s) 61
HindIII AAGCTT 2 cut(s) 1055, 1143
HpaII CCGG 3 cut(s) 64, 290, 1672
HphI GGTGA 7 cut(s) 29, 437, 449, 458, 686, 1194, 1515
Hpy166II GTNNAC 3 cut(s) 888, 932, 971
Hpy8I GTNNAC 3 cut(s) 888, 932, 971
Hpy99I CGWCG 1 cut(s) 1004
HpyAV CCTTC 4 cut(s) 249, 308, 491, 814
HpyCH4III ACNGT 6 cut(s) 115, 698, 776, 817, 910, 1479
HpyCH4IV ACGT 3 cut(s) 999, 1397, 1570
HpyCH4V TGCA 4 cut(s) 109, 1103, 1354, 1454
HpyF10VI GCNNNNNNNGC 2 cut(s) 1332, 1589
HpyF3I CTNAG 6 cut(s) 394, 399, 891, 921, 1376, 1605
HpySE526I ACGT 3 cut(s) 999, 1397, 1570
Hsp92II CATG 4 cut(s) 920, 930, 1454, 1513
HspAI GCGC 1 cut(s) 61
Kzo9I GATC 7 cut(s) 41, 691, 1425, 1432, 1468, 1492, 1531
LmnI GCTCC 7 cut(s) 130, 267, 302, 600, 620, 913, 1659
Lsp1109I GCAGC 1 cut(s) 1567
LweI GCATC 1 cut(s) 1069
MaeI CTAG 3 cut(s) 651, 755, 1696
MaeII ACGT 3 cut(s) 999, 1397, 1570
MaeIII GTNAC 2 cut(s) 904, 1521
MalI GATC 7 cut(s) 43, 693, 1427, 1434, 1470, 1494, 1533
MboI GATC 7 cut(s) 41, 691, 1425, 1432, 1468, 1492, 1531
MflI RGATCY 1 cut(s) 1531
MhlI GDGCHC 3 cut(s) 307, 1166, 1328
MlsI TGGCCA 1 cut(s) 366
MluNI TGGCCA 1 cut(s) 366
MlyI GAGTC 5 cut(s) 226, 790, 852, 1381, 1529
MmeI TCCRAC 1 cut(s) 985
Mox20I TGGCCA 1 cut(s) 366
Mph1103I ATGCAT 1 cut(s) 1456
MroXI GAANNNNTTC 2 cut(s) 824, 1041
MscI TGGCCA 1 cut(s) 366
MseI TTAA 5 cut(s) 188, 257, 540, 1221, 1394
MslI CAYNNNNRTG 2 cut(s) 21, 881
Msp20I TGGCCA 1 cut(s) 366
MspCI CTTAAG 1 cut(s) 187
MspI CCGG 3 cut(s) 64, 290, 1672
MspR9I CCNGG 4 cut(s) 966, 1388, 1404, 1673
MvaI CCWGG 3 cut(s) 966, 1388, 1404
MwoI GCNNNNNNNGC 2 cut(s) 1332, 1589
NciI CCSGG 1 cut(s) 1673
NdeII GATC 7 cut(s) 41, 691, 1425, 1432, 1468, 1492, 1531
NlaIII CATG 4 cut(s) 920, 930, 1454, 1513
NlaIV GGNNCC 5 cut(s) 132, 458, 525, 602, 1670
NmeAIII GCCGAG 1 cut(s) 76
NmuCI GTSAC 2 cut(s) 904, 1521
NsiI ATGCAT 1 cut(s) 1456
NspI RCATGY 3 cut(s) 920, 930, 1513
PaeI GCATGC 1 cut(s) 920
PciI ACATGT 1 cut(s) 926
PdmI GAANNNNTTC 2 cut(s) 824, 1041
PfeI GAWTC 8 cut(s) 151, 179, 245, 252, 377, 389, 674, 1439
Pfl23II CGTACG 1 cut(s) 1663
PflMI CCANNNNNTGG 1 cut(s) 1090
PkrI GCNGC 1 cut(s) 1582
PleI GAGTC 5 cut(s) 226, 790, 852, 1380, 1528
PpsI GAGTC 5 cut(s) 226, 790, 852, 1380, 1528
PscI ACATGT 1 cut(s) 926
Psp124BI GAGCTC 1 cut(s) 307
Psp1406I AACGTT 2 cut(s) 1397, 1570
Psp6I CCWGG 3 cut(s) 964, 1386, 1402
PspGI CCWGG 3 cut(s) 964, 1386, 1402
PspLI CGTACG 1 cut(s) 1663
PspN4I GGNNCC 5 cut(s) 132, 458, 525, 602, 1670
PspPI GGNCC 2 cut(s) 383, 456
PsuI RGATCY 1 cut(s) 1531
RsaI GTAC 5 cut(s) 710, 956, 1019, 1095, 1665
RsaNI GTAC 5 cut(s) 709, 955, 1018, 1094, 1664
RseI CAYNNNNRTG 2 cut(s) 21, 881
SacI GAGCTC 1 cut(s) 307
SaqAI TTAA 5 cut(s) 188, 257, 540, 1221, 1394
SatI GCNGC 1 cut(s) 1581
Sau3AI GATC 7 cut(s) 41, 691, 1425, 1432, 1468, 1492, 1531
Sau96I GGNCC 2 cut(s) 383, 456
SchI GAGTC 5 cut(s) 226, 790, 852, 1381, 1529
ScrFI CCNGG 4 cut(s) 966, 1388, 1404, 1673
SduI GDGCHC 3 cut(s) 307, 1166, 1328
SfaNI GCATC 1 cut(s) 1069
SfcI CTRYAG 2 cut(s) 1475, 1545
SinI GGWCC 1 cut(s) 456
SmiMI CAYNNNNRTG 2 cut(s) 21, 881
SmlI CTYRAG 4 cut(s) 140, 187, 419, 1637
SmoI CTYRAG 4 cut(s) 140, 187, 419, 1637
SphI GCATGC 1 cut(s) 920
SsiI CCGC 4 cut(s) 811, 862, 1281, 1590
SspMI CTAG 3 cut(s) 651, 755, 1696
SstI GAGCTC 1 cut(s) 307
StyD4I CCNGG 4 cut(s) 964, 1386, 1402, 1671
StyI CCWWGG 1 cut(s) 871
TaaI ACNGT 6 cut(s) 115, 698, 776, 817, 910, 1479
TaiI ACGT 3 cut(s) 1002, 1400, 1573
TaqI TCGA 2 cut(s) 1485, 1678
TatI WGTACW 2 cut(s) 708, 954
TfiI GAWTC 8 cut(s) 151, 179, 245, 252, 377, 389, 674, 1439
Tru1I TTAA 5 cut(s) 188, 257, 540, 1221, 1394
Tru9I TTAA 5 cut(s) 188, 257, 540, 1221, 1394
TscAI CASTG 5 cut(s) 118, 883, 915, 1118, 1636
TseFI GTSAC 2 cut(s) 904, 1521
TseI GCWGC 1 cut(s) 1580
Tsp45I GTSAC 2 cut(s) 904, 1521
TspDTI ATGAA 6 cut(s) 116, 224, 756, 832, 1155, 1293
TspGWI ACGGA 1 cut(s) 1681
TspRI CASTG 5 cut(s) 118, 883, 915, 1118, 1636
Van91I CCANNNNNTGG 1 cut(s) 1090
Vha464I CTTAAG 1 cut(s) 187
VpaK11BI GGWCC 1 cut(s) 456
XapI RAATTY 1 cut(s) 1417
XceI RCATGY 3 cut(s) 920, 930, 1513
XmiI GTMKAC 2 cut(s) 887, 931
XmnI GAANNNNTTC 2 cut(s) 824, 1041
XspI CTAG 3 cut(s) 651, 755, 1696
Zsp2I ATGCAT 1 cut(s) 1456
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.