Rroxscaffold_3G00273590

Calmodulin binding protein-like

Design Primers CRISPR Design

Basic Information

Type: gene
Biological Identity
rosa_roxburghii
GWHEROQ00000003
OG0004999
Physical Location & Seq
Forward (+)
65337103 .. 65341081
3979 bp
Loading structure...
UTR
Exon/CDS
Intron
Rroxscaffold_3G00273590.1

Sequence Viewer

Length: 1671 bp
ATGGAGTTTCATTCAGGTCGAACTGTCCCAGAAGCATCCCAGACGGGTCCAAATTCCTCTGATGACTGGCCACTGAAGAGACACCAGGATGATGGCAAGGGGAGTTATCGATCAGAAGGGACTGGTAGTCCGGGGGATAAGCGCCGGAGGACTACTTTTCAGAATGTGGTTTCGGAGATAATGAAGTTGCACACTGTCCAGCATTTGCTGGAGCCAATTCTTGAGCCTTTGATTCGCAGAGTGGTCAGAGAGGAAGTAGAACTGGCCCTAAGGAAACATTTGAACAATATGAAACAGAATTGTGAGAAAGAGACTCAACCTTCTGAATCAAGAATCTTAAAGCTACAGTTCTTAAACAGTCTGTCTCTTCCGGTATTTACTGGAGCTCGGATTGAAGGAGAAGAGAATTCCAGTATCCAAGTAGCTTTGATTGATTGCTTTACTGGCCAAATAGTTAAATCTGGCCCTGAATCCGCAGCTAAAGTGGAAATTGTTGTCCTTGAGGGTGATTTTGATGGTGAGGAGGGTGACAATTGGACCCCTGAAGAGTTCAAGAATAACATTGTAAGAGAGAGGGAAGGCAAAAAACCTCTTCTAACAGGGGAAGCAGTCATCAATCTTAATGATGGAATTGTTTCTGTGAGTGAGATTTCTTTTACAGATAATTCAAGCTGGACAAGGAGCCGTAGGTTCAGGTTAGGAGCAAGAGTTGTAGATAATTTTGATGGAACTAGAGTGAGAGAAGCAAAGACAGAATCCTTTATTGTCAGGGATCACCGTGGAGAATTGTACAAGAAGCACCACCCTCCATCTCTGCTTGATGAAGTATGGCGACTCGAAAAGATTGGAAAGGATGGAGCTTTCCATAAGCGTTTGACTCGGGAAAACATCCGCACCGTGAAGGATTTCCTCACCCTACTCTTCATAAACCCTCCAAGGCTCCGCCATATCCTTGGCACAGGTATGTCTACTAAGATGTGGGAAGTCACAGTGGAGCATGCTCAGACATGCATACTTGATAAGAGGATATACTTGTACTCCCCTCCCAGTTCACAACAGAGAACTGGTGTGGTCTTCAACGTTGTAGGACAAGTAACATACCTACTTTTGGAATGCGAATATATTCCTCTCAGTAAGCTTTCTGAAACACAAAAGGTTGATGCCCAGAACTTGGTACTTTCTGCATTTGAACACTGGGGAGAAGTAGTCTCATTTGATGATGAGGCTTCTCTTTTGGGTGGGGGCTCATCCAATGTCGCCAATGTTCATCAGACATCAAGTTCACCAAGAGGAGAGGAGTTTACTGGAAGCAATGGCTTGGATGATTATGCCTTGCACAACATTGATGGAATGGGTCTTAGATATGACCAGAGTTTAAGCTTCCCAGGTCAAGTTGGGAATTCCCTGATCTGTGATCCAGATTCAATAGCTGCATTTTGTGATGATGATCATCTACAGTTTTTTGATACTGATCTTCAGTCCCAGAGCATGCTTCCAGAGTCACCAGCAGATCTGCAAAGCGCTGTAGATGGCTTCTTGTTGGCACAACGTTCTACTGCTGCTGTTGCCGCCATAGCTCAGAGGAGATGGACAAAACTTTTCAGTGTCCTCAAGTGGTTCTCAATTCAGAGGAGCGTGCGGAACCGGGTTCGAGACATTCCAAGATACTAG

Protein Analysis

556

Amino Acids

62.66

Weight (kDa)

5.83

Isoelectric Point (pI)

49.34

Instability Index
Protein Domains (Pfam)
Domain Name Pfam ID Position E-value Description
Calmodulin_bind PF07887 113 - 259 1.7e-61 Calmodulin binding protein-like N-terminal domain
Calmod_bind_M PF20451 272 - 337 4.4e-32 Calmodulin binding protein central domain
Calmod_bind_C PF20452 343 - 404 2.5e-16 Calmodulin binding protein C-terminal domain
Hydrophobicity Profile (Kyte-Doolittle)
AI Structure Prediction Report
Calculating structure properties...

Genomic Context

Gene Family Tree

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Restriction Enzyme Sites

1 / 10
Enzyme Recognition Site Cut Count Positions (bp)
AccB7I CCANNNNNTGG 1 cut(s) 1171
AccI GTMKAC 1 cut(s) 968
AciI CCGC 5 cut(s) 474, 892, 943, 1569, 1639
AclI AACGTT 2 cut(s) 1080, 1549
AclWI GGATC 2 cut(s) 780, 1409
AcoI YGGCCR 2 cut(s) 68, 445
AcsI RAATTY 3 cut(s) 52, 406, 1399
AcuI CTGAAG 3 cut(s) 95, 564, 1460
AfaI GTAC 3 cut(s) 791, 1037, 1176
AfeI AGCGCT 1 cut(s) 1522
AfiI CCNNNNNNNGG 2 cut(s) 1108, 1171
AgsI TTSAA 7 cut(s) 283, 395, 553, 669, 1078, 1190, 1425
AhdI GACNNNNNGTC 1 cut(s) 126
AjnI CCWGG 2 cut(s) 84, 1384
Alw21I GWGCWC 1 cut(s) 388
Alw26I GTCTC 5 cut(s) 73, 305, 369, 1213, 1647
AlwI GGATC 2 cut(s) 780, 1409
Ama87I CYCGRG 1 cut(s) 879
Aor51HI AGCGCT 1 cut(s) 1522
AoxI GGCC 4 cut(s) 68, 264, 445, 463
ApeKI GCWGC 3 cut(s) 476, 1430, 1559
ApoI RAATTY 3 cut(s) 52, 406, 1399
Asp700I GAANNNNTTC 3 cut(s) 634, 905, 1122
AspLEI GCGC 2 cut(s) 144, 1523
AspS9I GGNCC 4 cut(s) 47, 265, 464, 537
AsuC2I CCSGG 2 cut(s) 132, 1646
AsuHPI GGTGA 7 cut(s) 518, 530, 539, 767, 904, 1275, 1494
AvaI CYCGRG 1 cut(s) 879
AvaII GGWCC 2 cut(s) 47, 537
AxyI CCTNAGG 1 cut(s) 269
BalI TGGCCA 2 cut(s) 70, 447
BanII GRGCYC 2 cut(s) 388, 1247
BbsI GAAGAC 1 cut(s) 1066
Bbv12I GWGCWC 1 cut(s) 388
BbvI GCAGC 3 cut(s) 488, 1417, 1546
BccI CCATC 9 cut(s) 86, 509, 620, 719, 817, 848, 1340, 1523, 1581
BceAI ACGGC 1 cut(s) 669
BciT130I CCWGG 2 cut(s) 86, 1386
BciVI GTATCC 1 cut(s) 425
BclI TGATCA 1 cut(s) 1447
BcnI CCSGG 2 cut(s) 132, 1646
BcoDI GTCTC 5 cut(s) 73, 305, 369, 1213, 1647
BfaI CTAG 2 cut(s) 732, 1669
BfmI CTRYAG 3 cut(s) 344, 1454, 1524
BfoI RGCGCY 2 cut(s) 145, 1524
BfuI GTATCC 1 cut(s) 425
BglII AGATCT 1 cut(s) 1510
BisI GCNGC 4 cut(s) 477, 1431, 1560, 1569
BlsI GCNGC 4 cut(s) 478, 1432, 1561, 1570
Bme1390I CCNGG 4 cut(s) 86, 132, 1386, 1646
Bme18I GGWCC 2 cut(s) 47, 537
BmeRI GACNNNNNGTC 1 cut(s) 126
BmeT110I CYCGRG 1 cut(s) 879
BmgT120I GGNCC 4 cut(s) 47, 265, 464, 537
BmiI GGNNCC 6 cut(s) 48, 213, 539, 683, 941, 1643
BmrFI CCNGG 4 cut(s) 86, 132, 1386, 1646
BmrI ACTGGG 2 cut(s) 1041, 1204
BmsI GCATC 2 cut(s) 44, 1150
BmuI ACTGGG 2 cut(s) 1041, 1204
BpiI GAAGAC 1 cut(s) 1066
BpmI CTGGAG 2 cut(s) 230, 402
BpuEI CTTGAG 3 cut(s) 242, 521, 1595
BpuMI CCSGG 2 cut(s) 132, 1646
Bsa29I ATCGAT 1 cut(s) 109
BsaBI GATNNNNATC 3 cut(s) 1446, 1449, 1470
BsaJI CCNNGG 5 cut(s) 131, 778, 935, 952, 1384
BsaWI WCCGGW 1 cut(s) 370
BsaXI ACNNNNNCTCC 6 cut(s) 693, 723, 1022, 1052, 1191, 1221
Bsc4I CCNNNNNNNGG 2 cut(s) 1108, 1171
Bse21I CCTNAGG 1 cut(s) 269
Bse3DI GCAATG 1 cut(s) 1318
Bse8I GATNNNNATC 3 cut(s) 1446, 1449, 1470
BseBI CCWGG 2 cut(s) 86, 1386
BseCI ATCGAT 1 cut(s) 109
BseDI CCNNGG 5 cut(s) 131, 778, 935, 952, 1384
BseGI GGATG 6 cut(s) 35, 94, 859, 888, 1247, 1327
BseJI GATNNNNATC 3 cut(s) 1446, 1449, 1470
BseLI CCNNNNNNNGG 2 cut(s) 1108, 1171
BseMI GCAATG 1 cut(s) 1318
BseMII CTCAG 3 cut(s) 1016, 1144, 1592
BseRI GAGGAG 5 cut(s) 536, 1305, 1310, 1597, 1645
BseXI GCAGC 3 cut(s) 488, 1417, 1546
BshFI GGCC 4 cut(s) 70, 266, 447, 465
BshVI ATCGAT 1 cut(s) 109
BsiHKAI GWGCWC 1 cut(s) 388
BsiHKCI CYCGRG 1 cut(s) 879
BsiSI CCGG 4 cut(s) 131, 145, 371, 1645
BslFI GGGAC 3 cut(s) 11, 133, 1465
BslI CCNNNNNNNGG 2 cut(s) 1108, 1171
BsmAI GTCTC 5 cut(s) 73, 305, 369, 1213, 1647
BsmFI GGGAC 3 cut(s) 11, 133, 1465
BsmI GAATGC 1 cut(s) 1118
BsnI GGCC 4 cut(s) 70, 266, 447, 465
BsoBI CYCGRG 1 cut(s) 879
Bsp1286I GDGCHC 2 cut(s) 388, 1247
Bsp1407I TGTACA 1 cut(s) 789
Bsp143I GATC 7 cut(s) 110, 772, 1407, 1414, 1447, 1471, 1510
BspACI CCGC 5 cut(s) 474, 892, 943, 1569, 1639
BspANI GGCC 4 cut(s) 70, 266, 447, 465
BspCNI CTCAG 3 cut(s) 1015, 1143, 1591
BspDI ATCGAT 1 cut(s) 109
BspLI GGNNCC 6 cut(s) 48, 213, 539, 683, 941, 1643
BspPI GGATC 2 cut(s) 780, 1409
BsrDI GCAATG 1 cut(s) 1318
BsrGI TGTACA 1 cut(s) 789
BssECI CCNNGG 5 cut(s) 131, 778, 935, 952, 1384
BssMI GATC 7 cut(s) 110, 772, 1407, 1414, 1447, 1471, 1510
BssT1I CCWWGG 2 cut(s) 935, 952
Bst2UI CCWGG 2 cut(s) 86, 1386
Bst4CI ACNGT 8 cut(s) 25, 196, 348, 359, 779, 898, 991, 1458
Bst6I CTCTTC 6 cut(s) 71, 372, 396, 540, 597, 926
BstAUI TGTACA 1 cut(s) 789
BstC8I GCNNGC 3 cut(s) 999, 1490, 1637
BstDEI CTNAG 6 cut(s) 269, 972, 1002, 1130, 1358, 1578
BstDSI CCRYGG 1 cut(s) 778
BstF5I GGATG 6 cut(s) 35, 94, 859, 888, 1247, 1327
BstH2I RGCGCY 2 cut(s) 145, 1524
BstHHI GCGC 2 cut(s) 144, 1523
BstKTI GATC 7 cut(s) 113, 775, 1410, 1417, 1450, 1474, 1513
BstMAI GTCTC 5 cut(s) 73, 305, 369, 1213, 1647
BstMBI GATC 7 cut(s) 110, 772, 1407, 1414, 1447, 1471, 1510
BstMWI GCNNNNNNNGC 4 cut(s) 444, 1565, 1568, 1574
BstNI CCWGG 2 cut(s) 86, 1386
BstNSI RCATGY 3 cut(s) 1001, 1011, 1492
BstSCI CCNGG 4 cut(s) 84, 130, 1384, 1644
BstSFI CTRYAG 3 cut(s) 344, 1454, 1524
BstV1I GCAGC 3 cut(s) 488, 1417, 1546
BstV2I GAAGAC 1 cut(s) 1066
BstX2I RGATCY 1 cut(s) 1510
BstXI CCANNNNNNTGG 2 cut(s) 92, 953
BstYI RGATCY 1 cut(s) 1510
Bsu15I ATCGAT 1 cut(s) 109
Bsu36I CCTNAGG 1 cut(s) 269
BsuI GTATCC 1 cut(s) 425
BsuRI GGCC 4 cut(s) 70, 266, 447, 465
BsuTUI ATCGAT 1 cut(s) 109
BtgI CCRYGG 1 cut(s) 778
BtsCI GGATG 6 cut(s) 35, 94, 859, 888, 1247, 1327
BtsIMutI CAGTG 5 cut(s) 71, 192, 996, 1192, 1609
Cac8I GCNNGC 3 cut(s) 999, 1490, 1637
CfoI GCGC 2 cut(s) 144, 1523
Cfr13I GGNCC 4 cut(s) 47, 265, 464, 537
ClaI ATCGAT 1 cut(s) 109
Csp6I GTAC 3 cut(s) 790, 1036, 1175
CviAII CATG 3 cut(s) 998, 1008, 1489
CviQI GTAC 3 cut(s) 790, 1036, 1175
DdeI CTNAG 6 cut(s) 269, 972, 1002, 1130, 1358, 1578
DpnI GATC 7 cut(s) 112, 774, 1409, 1416, 1449, 1473, 1512
DpnII GATC 7 cut(s) 110, 772, 1407, 1414, 1447, 1471, 1510
DriI GACNNNNNGTC 1 cut(s) 126
EaeI YGGCCR 2 cut(s) 68, 445
Eam1104I CTCTTC 6 cut(s) 71, 372, 396, 540, 597, 926
Eam1105I GACNNNNNGTC 1 cut(s) 126
EarI CTCTTC 6 cut(s) 71, 372, 396, 540, 597, 926
EciI GGCGGA 1 cut(s) 932
Ecl136II GAGCTC 1 cut(s) 386
Eco130I CCWWGG 2 cut(s) 935, 952
Eco24I GRGCYC 2 cut(s) 388, 1247
Eco47I GGWCC 2 cut(s) 47, 537
Eco47III AGCGCT 1 cut(s) 1522
Eco53kI GAGCTC 1 cut(s) 386
Eco57I CTGAAG 3 cut(s) 95, 564, 1460
Eco81I CCTNAGG 1 cut(s) 269
Eco88I CYCGRG 1 cut(s) 879
EcoICRI GAGCTC 1 cut(s) 386
EcoRI GAATTC 2 cut(s) 406, 1399
EcoRII CCWGG 2 cut(s) 84, 1384
EcoT14I CCWWGG 2 cut(s) 935, 952
EcoT22I ATGCAT 1 cut(s) 1013
EcoT38I GRGCYC 2 cut(s) 388, 1247
ErhI CCWWGG 2 cut(s) 935, 952
FaeI CATG 3 cut(s) 1001, 1011, 1492
FaqI GGGAC 3 cut(s) 11, 133, 1465
FatI CATG 3 cut(s) 997, 1007, 1488
FbaI TGATCA 1 cut(s) 1447
FblI GTMKAC 1 cut(s) 968
Fnu4HI GCNGC 4 cut(s) 477, 1431, 1560, 1569
FokI GGATG 6 cut(s) 22, 101, 866, 875, 1234, 1334
FriOI GRGCYC 2 cut(s) 388, 1247
Fsp4HI GCNGC 4 cut(s) 477, 1431, 1560, 1569
FspBI CTAG 2 cut(s) 732, 1669
GlaI GCGC 2 cut(s) 143, 1522
GluI GCNGC 4 cut(s) 477, 1431, 1560, 1569
GsuI CTGGAG 2 cut(s) 230, 402
HaeII RGCGCY 2 cut(s) 145, 1524
HaeIII GGCC 4 cut(s) 70, 266, 447, 465
HapII CCGG 4 cut(s) 131, 145, 371, 1645
HhaI GCGC 2 cut(s) 144, 1523
Hin1II CATG 3 cut(s) 1001, 1011, 1492
Hin6I GCGC 2 cut(s) 142, 1521
HinP1I GCGC 2 cut(s) 142, 1521
HindIII AAGCTT 2 cut(s) 1136, 1378
HpaII CCGG 4 cut(s) 131, 145, 371, 1645
HphI GGTGA 7 cut(s) 518, 530, 539, 767, 904, 1275, 1494
Hpy166II GTNNAC 4 cut(s) 969, 1052, 1283, 1302
Hpy188III TCNNGA 7 cut(s) 221, 330, 553, 881, 1418, 1496, 1652
Hpy8I GTNNAC 4 cut(s) 969, 1052, 1283, 1302
HpyAV CCTTC 5 cut(s) 110, 330, 389, 572, 895
HpyCH4III ACNGT 8 cut(s) 25, 196, 348, 359, 779, 898, 991, 1458
HpyCH4IV ACGT 2 cut(s) 1080, 1549
HpyCH4V TGCA 6 cut(s) 190, 1011, 1184, 1336, 1433, 1516
HpyF10VI GCNNNNNNNGC 4 cut(s) 444, 1565, 1568, 1574
HpyF3I CTNAG 6 cut(s) 269, 972, 1002, 1130, 1358, 1578
HpySE526I ACGT 2 cut(s) 1080, 1549
Hsp92II CATG 3 cut(s) 1001, 1011, 1492
HspAI GCGC 2 cut(s) 142, 1521
Ksp22I TGATCA 1 cut(s) 1447
Kzo9I GATC 7 cut(s) 110, 772, 1407, 1414, 1447, 1471, 1510
LmnI GCTCC 8 cut(s) 211, 383, 681, 701, 857, 945, 994, 1632
Lsp1109I GCAGC 3 cut(s) 488, 1417, 1546
LweI GCATC 2 cut(s) 44, 1150
MaeI CTAG 2 cut(s) 732, 1669
MaeII ACGT 2 cut(s) 1080, 1549
MaeIII GTNAC 4 cut(s) 527, 985, 1093, 1500
MalI GATC 7 cut(s) 112, 774, 1409, 1416, 1449, 1473, 1512
MboI GATC 7 cut(s) 110, 772, 1407, 1414, 1447, 1471, 1510
MboII GAAGA 8 cut(s) 88, 359, 413, 557, 584, 913, 1066, 1466
MfeI CAATTG 1 cut(s) 532
MflI RGATCY 1 cut(s) 1510
MhlI GDGCHC 2 cut(s) 388, 1247
MlsI TGGCCA 2 cut(s) 70, 447
MluNI TGGCCA 2 cut(s) 70, 447
MlyI GAGTC 4 cut(s) 307, 828, 871, 1508
Mox20I TGGCCA 2 cut(s) 70, 447
Mph1103I ATGCAT 1 cut(s) 1013
MroXI GAANNNNTTC 3 cut(s) 634, 905, 1122
MscI TGGCCA 2 cut(s) 70, 447
MseI TTAA 5 cut(s) 338, 353, 456, 621, 1376
MslI CAYNNNNRTG 2 cut(s) 87, 962
Msp20I TGGCCA 2 cut(s) 70, 447
MspI CCGG 4 cut(s) 131, 145, 371, 1645
MspR9I CCNGG 4 cut(s) 86, 132, 1386, 1646
MunI CAATTG 1 cut(s) 532
Mva1269I GAATGC 1 cut(s) 1118
MvaI CCWGG 2 cut(s) 86, 1386
MwoI GCNNNNNNNGC 4 cut(s) 444, 1565, 1568, 1574
NciI CCSGG 2 cut(s) 132, 1646
NdeII GATC 7 cut(s) 110, 772, 1407, 1414, 1447, 1471, 1510
NlaIII CATG 3 cut(s) 1001, 1011, 1492
NlaIV GGNNCC 6 cut(s) 48, 213, 539, 683, 941, 1643
NmuCI GTSAC 3 cut(s) 527, 985, 1500
NsiI ATGCAT 1 cut(s) 1013
NspI RCATGY 3 cut(s) 1001, 1011, 1492
PaeI GCATGC 2 cut(s) 1001, 1492
PctI GAATGC 1 cut(s) 1118
PdmI GAANNNNTTC 3 cut(s) 634, 905, 1122
PfeI GAWTC 6 cut(s) 232, 326, 333, 470, 755, 1421
PflMI CCANNNNNTGG 1 cut(s) 1171
PkrI GCNGC 4 cut(s) 478, 1432, 1561, 1570
PleI GAGTC 4 cut(s) 307, 828, 871, 1507
PpsI GAGTC 4 cut(s) 307, 828, 871, 1507
Psp124BI GAGCTC 1 cut(s) 388
Psp1406I AACGTT 2 cut(s) 1080, 1549
Psp6I CCWGG 2 cut(s) 84, 1384
PspGI CCWGG 2 cut(s) 84, 1384
PspN4I GGNNCC 6 cut(s) 48, 213, 539, 683, 941, 1643
PspPI GGNCC 4 cut(s) 47, 265, 464, 537
PsuI RGATCY 1 cut(s) 1510
RsaI GTAC 3 cut(s) 791, 1037, 1176
RsaNI GTAC 3 cut(s) 790, 1036, 1175
RseI CAYNNNNRTG 2 cut(s) 87, 962
SacI GAGCTC 1 cut(s) 388
SaqAI TTAA 5 cut(s) 338, 353, 456, 621, 1376
SatI GCNGC 4 cut(s) 477, 1431, 1560, 1569
Sau3AI GATC 7 cut(s) 110, 772, 1407, 1414, 1447, 1471, 1510
Sau96I GGNCC 4 cut(s) 47, 265, 464, 537
SchI GAGTC 4 cut(s) 307, 828, 871, 1508
ScrFI CCNGG 4 cut(s) 86, 132, 1386, 1646
SduI GDGCHC 2 cut(s) 388, 1247
SfaNI GCATC 2 cut(s) 44, 1150
SfcI CTRYAG 3 cut(s) 344, 1454, 1524
SinI GGWCC 2 cut(s) 47, 537
SmiMI CAYNNNNRTG 2 cut(s) 87, 962
SmlI CTYRAG 3 cut(s) 221, 500, 1610
SmoI CTYRAG 3 cut(s) 221, 500, 1610
SphI GCATGC 2 cut(s) 1001, 1492
SsiI CCGC 5 cut(s) 474, 892, 943, 1569, 1639
SspMI CTAG 2 cut(s) 732, 1669
SstI GAGCTC 1 cut(s) 388
StyD4I CCNGG 4 cut(s) 84, 130, 1384, 1644
StyI CCWWGG 2 cut(s) 935, 952
TaaI ACNGT 8 cut(s) 25, 196, 348, 359, 779, 898, 991, 1458
TaiI ACGT 2 cut(s) 1083, 1552
TaqI TCGA 4 cut(s) 19, 109, 837, 1651
TatI WGTACW 2 cut(s) 789, 1035
TauI GCSGC 1 cut(s) 1571
TfiI GAWTC 6 cut(s) 232, 326, 333, 470, 755, 1421
Tru1I TTAA 5 cut(s) 338, 353, 456, 621, 1376
Tru9I TTAA 5 cut(s) 338, 353, 456, 621, 1376
TscAI CASTG 5 cut(s) 78, 199, 996, 1199, 1609
TseFI GTSAC 3 cut(s) 527, 985, 1500
TseI GCWGC 3 cut(s) 476, 1430, 1559
Tsp45I GTSAC 3 cut(s) 527, 985, 1500
TspDTI ATGAA 5 cut(s) 197, 305, 837, 913, 1256
TspRI CASTG 5 cut(s) 78, 199, 996, 1199, 1609
Van91I CCANNNNNTGG 1 cut(s) 1171
VpaK11BI GGWCC 2 cut(s) 47, 537
XapI RAATTY 3 cut(s) 52, 406, 1399
XceI RCATGY 3 cut(s) 1001, 1011, 1492
XmiI GTMKAC 1 cut(s) 968
XmnI GAANNNNTTC 3 cut(s) 634, 905, 1122
XspI CTAG 2 cut(s) 732, 1669
Zsp2I ATGCAT 1 cut(s) 1013
Using CommOnly database (standard laboratory enzymes). Scanned on CDS sequence.